Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,425,773 A→G G13G (GGT→GGC)  argT ← ArgT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,425,7730AG98.2% 511.1 / ‑1.3 164G13G (GGT→GGC) argTArgT
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (71/90);  total (73/91)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 171 total aligned reads displayed.

ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGT  >  NC_000913/2425683‑2425862
                                                                                          |                                                                                         
aTCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGcc                                                                                         >  1:2452482/1‑93 (MQ=255)
aTCTTGCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGcc                                                                                         <  2:2775837/93‑1 (MQ=255)
 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                        >  1:1282098/1‑93 (MQ=255)
 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                        >  2:2675847/1‑93 (MQ=255)
  cTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGa                                                                                       >  1:3871338/1‑93 (MQ=255)
       gATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAAc                                                                                  >  1:4462039/1‑93 (MQ=255)
        aTGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACa                                                                                 >  1:3716712/1‑93 (MQ=255)
        aTGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACa                                                                                 >  1:2217071/1‑93 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                                >  2:3736775/1‑93 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                                >  1:4206065/1‑93 (MQ=255)
         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                                <  1:3288258/93‑1 (MQ=255)
          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                               >  2:197027/1‑93 (MQ=255)
          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                               >  1:3554100/1‑93 (MQ=255)
          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                               <  2:4377552/93‑1 (MQ=255)
           agaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAg                                                                              >  2:4011161/1‑93 (MQ=255)
             aaCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGAc                                                                            <  1:4066523/93‑1 (MQ=255)
              aCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACa                                                                           <  1:1722030/93‑1 (MQ=255)
               cGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACag                                                                          >  1:679557/1‑93 (MQ=255)
                ggTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACaga                                                                         <  2:3201286/93‑1 (MQ=255)
                  tGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGc                                                                       <  1:1231123/93‑1 (MQ=255)
                  tGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGc                                                                       <  1:516921/93‑1 (MQ=255)
                   gCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCg                                                                      <  1:2388159/93‑1 (MQ=255)
                   gCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCg                                                                      >  2:2648599/1‑93 (MQ=255)
                   gCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCg                                                                      >  2:1303235/1‑93 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGGCGACTAACAAAGACAGAGCga                                                                     >  2:1521933/1‑93 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                     >  2:4602469/1‑93 (MQ=255)
                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCga                                                                     >  1:3003184/1‑93 (MQ=255)
                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                  <  1:4486794/93‑1 (MQ=255)
                         gtggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATc                                                                <  2:4687808/93‑1 (MQ=255)
                         gtggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATc                                                                <  1:1572064/93‑1 (MQ=255)
                         gtggtAGCGCTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATc                                                                <  2:2184348/93‑1 (MQ=255)
                          tggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCg                                                               <  2:3745525/93‑1 (MQ=255)
                           ggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGa                                                              >  1:1320805/1‑93 (MQ=255)
                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActt                                                           <  1:1958650/93‑1 (MQ=255)
                               tCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                          <  1:2993818/93‑1 (MQ=255)
                                cGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtct                                                         <  1:2034421/93‑1 (MQ=255)
                                cGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtct                                                         >  1:895044/1‑93 (MQ=255)
                                 ggTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtctt                                                        <  1:2955786/93‑1 (MQ=255)
                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                       <  2:13435/93‑1 (MQ=255)
                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                       >  2:2162058/1‑93 (MQ=255)
                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                       <  2:2724020/93‑1 (MQ=255)
                                   ttCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTca                                                      <  2:3619400/93‑1 (MQ=255)
                                    tCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcat                                                     <  1:2068798/93‑1 (MQ=255)
                                    tCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcat                                                     <  2:2318344/93‑1 (MQ=255)
                                     ccGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcata                                                    >  2:4174811/1‑93 (MQ=255)
                                      cGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                   >  2:3488452/1‑93 (MQ=255)
                                      cGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatac                                                   >  2:2807556/1‑93 (MQ=255)
                                       gATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcataca                                                  <  1:3047099/93‑1 (MQ=255)
                                       gATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcataca                                                  >  2:915870/1‑93 (MQ=255)
                                       gATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcataca                                                  >  2:1815212/1‑93 (MQ=255)
                                        aTACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGGCAGAGCGAGAATCGACTTCTTcatacat                                                 <  2:596916/93‑1 (MQ=255)
                                        aTACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacat                                                 >  2:1513837/1‑93 (MQ=255)
                                            gTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATATcc                                             <  2:4208210/93‑1 (MQ=255)
                                             tACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGCCTTCTTCATACATAAccc                                            >  1:3435318/1‑93 (MQ=255)
                                             tACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAccc                                            <  2:3129686/93‑1 (MQ=255)
                                             tACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAccc                                            >  1:2878245/1‑93 (MQ=255)
                                             tACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAccc                                            >  1:3653965/1‑93 (MQ=255)
                                                cGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCa                                         >  1:3977230/1‑93 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                       <  1:2348536/93‑1 (MQ=255)
                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                       >  2:213425/1‑93 (MQ=255)
                                                   ctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGt                                      <  2:1471131/93‑1 (MQ=255)
                                                   ctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGt                                      <  1:1900226/93‑1 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                     <  1:1330357/93‑1 (MQ=255)
                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATAAATAACCCTCAAGTg                                     >  1:2229298/1‑93 (MQ=255)
                                                      cGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaa                                   <  2:4163701/93‑1 (MQ=255)
                                                      cGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaa                                   >  2:4132650/1‑93 (MQ=255)
                                                       ggTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaa                                  <  1:813879/93‑1 (MQ=255)
                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                 <  1:3815317/93‑1 (MQ=255)
                                                         tAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAt                                <  1:2145749/93‑1 (MQ=255)
                                                          aGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATg                               <  2:757514/93‑1 (MQ=255)
                                                           gcgcCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATgg                              >  1:907546/1‑93 (MQ=255)
                                                            cgccgcATAGCTGGAAGCAGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGc                             <  2:2360683/93‑1 (MQ=255)
                                                             gccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCt                            <  2:1633582/93‑1 (MQ=255)
                                                             gccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCt                            >  1:2123108/1‑93 (MQ=255)
                                                             gccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCt                            >  2:3279175/1‑93 (MQ=255)
                                                              ccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtt                           <  2:1030784/93‑1 (MQ=255)
                                                                gcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttt                         <  2:3866014/93‑1 (MQ=255)
                                                                gcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttt                         <  2:1895991/93‑1 (MQ=255)
                                                                gcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttt                         <  1:3920986/93‑1 (MQ=255)
                                                                 cATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttttt                        <  1:2561931/93‑1 (MQ=255)
                                                                  aTAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttttt                       <  1:327871/93‑1 (MQ=255)
                                                                  aTAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttttt                       >  1:3728817/1‑93 (MQ=255)
                                                                   tAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTa                      <  1:2803465/93‑1 (MQ=255)
                                                                    aGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAt                     <  1:749647/93‑1 (MQ=255)
                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                 <  1:1155110/93‑1 (MQ=255)
                                                                         gAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttgt                <  2:2485056/93‑1 (MQ=255)
                                                                         gAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttgt                >  2:4191300/1‑93 (MQ=255)
                                                                          aaGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTtgtg               >  2:2549700/1‑93 (MQ=255)
                                                                          aaGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTtgtg               >  1:2701978/1‑93 (MQ=255)
                                                                            gCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTa             <  1:3618309/93‑1 (MQ=255)
                                                                              cGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAtt           <  2:1717981/93‑1 (MQ=255)
                                                                               gCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttg          >  1:4712874/1‑93 (MQ=255)
                                                                               gCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttg          <  1:125380/93‑1 (MQ=255)
                                                                               gCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttg          <  2:1260789/93‑1 (MQ=255)
                                                                                 tgtgGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtg        >  2:4728248/1‑93 (MQ=255)
                                                                                  gtgGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgt       <  1:1875425/93‑1 (MQ=255)
                                                                                   tgGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCACAAGTGAAAATGGCTTTTTTATGTTGTGAAttgtgta      <  2:4339293/93‑2 (MQ=255)
                                                                                     gagaggCCGACTAACAAAGACAGATCGAGAATCGACTTCTTCATACATAACCCTCAAGGGAAAATGGCTTTTTTATGTTGTGTAttgtgttgt    <  2:663566/93‑1 (MQ=255)
                                                                                      agaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttgtg   <  1:1027215/93‑1 (MQ=255)
                                                                                       gaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATtgtgttgtgt  <  1:1271455/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGT  >  NC_000913/2425683‑2425862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425680‑2425866
                                                                                             |                                                                                             
AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGG                                                                                               <  SRR4302544.4212733/94‑1 (MQ=60)
AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCAGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGG                                                                                               >  SRR4302544.1920925/1‑94 (MQ=60)
AGCATCTTTCGATGAGAACGGTGCGTAGGTGGGATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGG                                                                                               >  SRR4302544.1920926/1‑94 (MQ=60)
 GCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGC                                                                                              >  SRR4302544.2796670/1‑94 (MQ=60)
  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             >  SRR4302544.2475876/1‑94 (MQ=60)
  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             <  SRR4302544.3295413/94‑1 (MQ=60)
  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             <  SRR4302544.512405/94‑1 (MQ=60)
   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            >  SRR4302544.2395170/1‑94 (MQ=60)
   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            <  SRR4302544.529502/94‑1 (MQ=60)
   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            >  SRR4302544.1294731/1‑94 (MQ=60)
   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            >  SRR4302544.3053235/1‑94 (MQ=60)
   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            >  SRR4302544.1608547/1‑94 (MQ=60)
    TCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGA                                                                                           >  SRR4302544.3909783/1‑94 (MQ=60)
     CTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAC                                                                                          >  SRR4302544.4147113/1‑94 (MQ=60)
      TTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACT                                                                                         <  SRR4302544.299841/94‑1 (MQ=60)
         CGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAAC                                                                                      >  SRR4302544.4506947/1‑94 (MQ=60)
          GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302544.2237924/1‑94 (MQ=60)
          GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302544.3753685/1‑94 (MQ=60)
           ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    >  SRR4302544.4248262/1‑94 (MQ=60)
           ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    >  SRR4302544.2116683/1‑94 (MQ=60)
            TGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAA                                                                                   >  SRR4302544.3588150/1‑94 (MQ=60)
            TGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAA                                                                                   <  SRR4302544.3319587/94‑1 (MQ=60)
             GAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAG                                                                                  <  SRR4302544.3092270/94‑1 (MQ=60)
             GAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAG                                                                                  >  SRR4302544.440355/1‑94 (MQ=60)
              AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGA                                                                                 >  SRR4302544.775183/1‑94 (MQ=60)
                AACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACA                                                                               <  SRR4302544.4107010/94‑1 (MQ=60)
                 ACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAG                                                                              >  SRR4302544.686297/1‑94 (MQ=60)
                 ACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAG                                                                              <  SRR4302544.1738940/94‑1 (MQ=60)
                  CGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGA                                                                             >  SRR4302544.2488819/1‑94 (MQ=60)
                   GGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAG                                                                            <  SRR4302544.1298143/94‑1 (MQ=60)
                     TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          >  SRR4302544.3752312/1‑94 (MQ=60)
                     TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          <  SRR4302544.3535491/94‑1 (MQ=60)
                     TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          <  SRR4302544.522033/94‑1 (MQ=60)
                     TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          <  SRR4302544.1243301/94‑1 (MQ=60)
                      GCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGA                                                                         <  SRR4302544.2410773/94‑1 (MQ=60)
                      GCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGA                                                                         >  SRR4302544.3031971/1‑94 (MQ=60)
                        GTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGA                                                                       >  SRR4302544.1243590/1‑94 (MQ=60)
                          AGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAAT                                                                     <  SRR4302544.4531862/94‑1 (MQ=60)
                           GGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATC                                                                    <  SRR4302544.177030/94‑1 (MQ=60)
                           GGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATC                                                                    <  SRR4302544.3764583/94‑1 (MQ=60)
                            GTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCG                                                                   <  SRR4302544.1587611/94‑1 (MQ=60)
                             TGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGA                                                                  >  SRR4302544.1333795/1‑94 (MQ=60)
                                 ATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTC                                                              >  SRR4302544.3591928/1‑94 (MQ=60)
                                 ATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTC                                                              <  SRR4302544.1977654/94‑1 (MQ=60)
                                  TCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             >  SRR4302544.903896/1‑94 (MQ=60)
                                  TCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             <  SRR4302544.3022521/94‑1 (MQ=60)
                                   CGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTT                                                            <  SRR4302544.2054092/94‑1 (MQ=60)
                                   CGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTT                                                            <  SRR4302544.87434/94‑1 (MQ=60)
                                   CGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTT                                                            <  SRR4302544.3974108/94‑1 (MQ=60)
                                    GGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTC                                                           <  SRR4302544.2984171/94‑1 (MQ=60)
                                    GGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTC                                                           >  SRR4302544.393776/1‑94 (MQ=60)
                                       TCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATA                                                        <  SRR4302544.2088661/94‑1 (MQ=60)
                                         CGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGACTCGACTTCTTCATACA                                                      >  SRR4302544.2924290/1‑94 (MQ=60)
                                          GATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACAT                                                     <  SRR4302544.3076223/94‑1 (MQ=60)
                                               GTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGCCTTCTTCATACATAACCC                                                >  SRR4302544.3468156/1‑94 (MQ=60)
                                               GTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCC                                                >  SRR4302544.2906024/1‑94 (MQ=60)
                                               GTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCC                                                >  SRR4302544.3690426/1‑94 (MQ=60)
                                                  CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             >  SRR4302544.4540181/1‑94 (MQ=60)
                                                  CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             >  SRR4302544.4016806/1‑94 (MQ=60)
                                                   CGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAA                                            >  SRR4302544.3316690/1‑94 (MQ=60)
                                                    GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           >  SRR4302544.3882761/1‑94 (MQ=60)
                                                     TCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302544.2042190/94‑1 (MQ=60)
                                                     TCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302544.2370782/94‑1 (MQ=60)
                                                      CTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTG                                         <  SRR4302544.1918585/94‑1 (MQ=60)
                                                      CTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTG                                         >  SRR4302544.5114/1‑94 (MQ=60)
                                                      CTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATAAATAACCCTCAAGTG                                         >  SRR4302544.2250273/1‑94 (MQ=60)
                                                       TCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGA                                        <  SRR4302544.1343449/94‑1 (MQ=60)
                                                          GGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAA                                     <  SRR4302544.821824/94‑1 (MQ=60)
                                                          GGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAA                                     >  SRR4302544.2690037/1‑94 (MQ=60)
                                                           GTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAT                                    <  SRR4302544.3853132/94‑1 (MQ=60)
                                                            TAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATG                                   <  SRR4302544.2166200/94‑1 (MQ=60)
                                                             AGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGG                                  >  SRR4302544.916518/1‑94 (MQ=60)
                                                             AGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGG                                  <  SRR4302544.1288053/94‑1 (MQ=60)
                                                              GCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGC                                 >  SRR4302544.1962212/1‑94 (MQ=60)
                                                               CGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCT                                <  SRR4302544.1499818/94‑1 (MQ=60)
                                                               CGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCT                                >  SRR4302544.2143478/1‑94 (MQ=60)
                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            <  SRR4302544.3959931/94‑1 (MQ=60)
                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            >  SRR4302544.2810281/1‑94 (MQ=60)
                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           <  SRR4302544.2191687/94‑1 (MQ=60)
                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           >  SRR4302544.3765892/1‑94 (MQ=60)
                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           <  SRR4302544.2586540/94‑1 (MQ=60)
                                                                     ATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTA                          <  SRR4302544.331279/94‑1 (MQ=60)
                                                                      TAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAT                         <  SRR4302544.2830489/94‑1 (MQ=60)
                                                                       AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        <  SRR4302544.756971/94‑1 (MQ=60)
                                                                         CTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTT                      >  SRR4302544.992860/1‑94 (MQ=60)
                                                                           GGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGT                    <  SRR4302544.1166599/94‑1 (MQ=60)
                                                                            GAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTG                   >  SRR4302544.2727958/1‑94 (MQ=60)
                                                                               GCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAT                <  SRR4302544.3654275/94‑1 (MQ=60)
                                                                                CCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATT               <  SRR4302544.1219272/94‑1 (MQ=60)
                                                                                 CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              >  SRR4302544.4759430/1‑94 (MQ=60)
                                                                                  GCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGT             <  SRR4302544.126536/94‑1 (MQ=60)
                                                                                     GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          <  SRR4302544.1893556/94‑1 (MQ=60)
                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         <  SRR4302544.2252053/94‑1 (MQ=60)
                                                                                         AGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGT      >  SRR4302544.2164760/1‑94 (MQ=60)
                                                                                         AGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGT      <  SRR4302544.1037460/94‑1 (MQ=60)
                                                                                          GAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTG     <  SRR4302544.1283963/94‑1 (MQ=60)
                                                                                           AGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT    >  SRR4302544.3919972/1‑94 (MQ=60)
                                                                                             GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  <  SRR4302544.3388205/94‑1 (MQ=60)
                                                                                             GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  <  SRR4302544.1477734/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425680‑2425866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑