Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,425,773	A→G	G13G (GGT→GGC)	argT ←	ArgT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,425,773	0	A	G	99.6%	809.6 / NA	253	G13G (GGT→GGC)	argT	ArgT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (108/144); minor base T (0/1); total (108/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT  >  NC_000913/2425683‑2425864

aTCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGcc                                                                                           <  1:836004/93‑1 (MQ=255)

 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                          >  2:4977072/1‑93 (MQ=255)

 tCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCg                                                                                          >  2:5752014/1‑93 (MQ=255)

   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                        <  2:6350034/93‑1 (MQ=255)

   tttCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGAc                                                                                        <  1:1911363/93‑1 (MQ=255)

       gATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCGGACTaca                                                                                    >  2:1198561/1‑91 (MQ=255)

       gATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTaaa                                                                                    >  2:5459106/1‑92 (MQ=255)

       gATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAAc                                                                                    <  2:649975/93‑1 (MQ=255)

       gATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAAc                                                                                    >  2:6697544/1‑93 (MQ=255)

        aTGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACa                                                                                   <  1:5529057/93‑1 (MQ=255)

        aTGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACa                                                                                   <  2:1519810/93‑1 (MQ=255)

         tGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaa                                                                                  <  2:6656297/93‑1 (MQ=255)

          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                                 <  2:142040/93‑1 (MQ=255)

          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                                 >  2:1106938/1‑93 (MQ=255)

          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                                 >  1:2231403/1‑93 (MQ=255)

          gagaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACaaa                                                                                 <  1:5799361/93‑1 (MQ=255)

           agaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAg                                                                                >  2:3396866/1‑93 (MQ=255)

           agaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAg                                                                                <  2:1335002/93‑1 (MQ=255)

            gtaCGGTGGGTGGGTGGTATCGGTTCGGATAAGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGTAGAGGCCGACTAACAAAGa                                                                               <  1:6544753/91‑1 (MQ=255)

            gaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGa                                                                               >  2:1049282/1‑93 (MQ=255)

            gaACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGa                                                                               >  1:2687577/1‑93 (MQ=255)

             aaCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGAc                                                                              <  2:4424252/93‑1 (MQ=255)

              aCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACa                                                                             <  2:3146605/93‑1 (MQ=255)

              aCGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACa                                                                             >  1:5928932/1‑93 (MQ=255)

               cGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACag                                                                            <  2:3487306/93‑1 (MQ=255)

                 gTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACagag                                                                          <  1:4384611/93‑1 (MQ=255)

                 gTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACagag                                                                          <  1:6216491/93‑1 (MQ=255)

                 gTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACagag                                                                          <  2:4059975/93‑1 (MQ=255)

                  tGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGc                                                                         >  2:2904543/1‑93 (MQ=255)

                   gCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCg                                                                        <  2:3334396/93‑1 (MQ=255)

                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                       <  2:2420517/93‑1 (MQ=255)

                    cGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCga                                                                       <  1:2480264/93‑1 (MQ=255)

                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                    <  1:6464058/93‑1 (MQ=255)

                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                    >  1:2120590/1‑93 (MQ=255)

                       aGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGaa                                                                    <  2:4118819/93‑1 (MQ=255)

                        ggtggtATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAAt                                                                   >  2:57104/1‑93 (MQ=255)

                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActt                                                             >  2:3891649/1‑93 (MQ=255)

                              aTCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGAcct                                                             >  2:2582649/1‑91 (MQ=255)

                               tCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                            <  2:2059744/93‑1 (MQ=255)

                               tCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                            <  1:1011801/93‑1 (MQ=255)

                               tCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                            <  1:152763/93‑1 (MQ=255)

                               tCGCTTCTGATACGTACGGCCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtc                                                            <  1:976023/93‑1 (MQ=255)

                                cGGTTCCGATACGTACCGTCTCCGGTAACGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGActtct                                                           >  2:2265885/1‑93 (MQ=255)

                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                         <  2:2789559/93‑1 (MQ=255)

                                  gTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACttcttc                                                         >  2:6232642/1‑93 (MQ=255)

                                     ccGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcata                                                      <  2:5350755/93‑1 (MQ=255)

                                       gATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcataca                                                    >  1:4758854/1‑93 (MQ=255)

                                         tACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTcatacata                                                  >  2:5254682/1‑93 (MQ=255)

                                           cGTCCCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                                <  1:2579838/93‑1 (MQ=255)

                                           cGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                                <  1:1947844/93‑1 (MQ=255)

                                           cGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                                <  2:2521467/93‑1 (MQ=255)

                                           cGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAc                                                <  2:250718/93‑1 (MQ=255)

                                             tACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAAccc                                              >  1:485969/1‑93 (MQ=255)

                                               ccGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTc                                            <  1:5478669/93‑1 (MQ=255)

                                                cGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCa                                           <  1:5056347/93‑1 (MQ=255)

                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                          <  1:208959/93‑1 (MQ=255)

                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                          >  2:4382557/1‑93 (MQ=255)

                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                          >  1:5640228/1‑93 (MQ=255)

                                                 gTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCaa                                          <  1:520006/93‑1 (MQ=255)

                                                  tctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                         <  2:3531424/93‑1 (MQ=255)

                                                  cctcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAg                                         <  1:5346938/92‑1 (MQ=255)

                                                    tcCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTg                                       <  2:3614357/93‑1 (MQ=255)

                                                     ccGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGa                                      >  2:5192305/1‑93 (MQ=255)

                                                       ggTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaa                                    >  2:1873873/1‑93 (MQ=255)

                                                       ggTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaa                                    <  1:1464893/93‑1 (MQ=255)

                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                   <  1:1257031/93‑1 (MQ=255)

                                                        gTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGaaaa                                   <  1:4005719/93‑1 (MQ=255)

                                                         tAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAt                                  <  2:3290036/93‑1 (MQ=255)

                                                           gcgcCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATgg                                >  1:3585315/1‑93 (MQ=255)

                                                           gcgcCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATgg                                <  1:3071417/93‑1 (MQ=255)

                                                             gccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCt                              <  2:1624355/93‑1 (MQ=255)

                                                              ccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtt                             <  1:3362147/93‑1 (MQ=255)

                                                              ccgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtt                             <  2:1658306/93‑1 (MQ=255)

                                                               cgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttt                            <  1:6257015/93‑1 (MQ=255)

                                                               cgcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttt                            >  2:2157965/1‑93 (MQ=255)

                                                                gcATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttt                           >  1:704007/1‑93 (MQ=255)

                                                                 cATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttttt                          >  1:327013/1‑93 (MQ=255)

                                                                 cATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCttttt                          >  2:4573059/1‑93 (MQ=255)

                                                                  aTAGCTGGAAGCCGCTGTGGAGAGGCCGACTAGCAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttttt                         <  1:4950527/93‑1 (MQ=255)

                                                                  aTAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttttt                         >  1:3891938/1‑93 (MQ=255)

                                                                  aTAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCtttttt                         >  1:5164930/1‑93 (MQ=255)

                                                                   tAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTa                        >  2:8329/1‑93 (MQ=255)

                                                                   tAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTa                        >  2:3204825/1‑93 (MQ=255)

                                                                   tAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTa                        <  1:2967601/93‑1 (MQ=255)

                                                                      cTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgt                     <  1:800581/93‑1 (MQ=255)

                                                                       tGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgtt                    <  1:3556424/93‑1 (MQ=255)

                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                   <  2:2679226/93‑1 (MQ=255)

                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                   <  1:1174425/93‑1 (MQ=255)

                                                                        ggAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAtgttg                   >  2:6009486/1‑93 (MQ=255)

                                                                          aaGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTtgtg                 >  1:6042327/1‑93 (MQ=255)

                                                                           aGCCGCTGTGGAGGGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCGTACATAACCCTCAAGGGAAAATGGCTTTTTTAtgttgggg                >  1:4584743/1‑90 (MQ=255)

                                                                           aGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTgtgt                >  2:4735452/1‑93 (MQ=255)

                                                                           aGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTgtgt                >  2:5774011/1‑93 (MQ=255)

                                                                              cGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAtt             <  2:2352222/93‑1 (MQ=255)

                                                                              cGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAtt             <  1:2000958/93‑1 (MQ=255)

                                                                               gCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttg            >  1:1637011/1‑93 (MQ=255)

                                                                                   tgGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgtt        <  1:4657742/93‑1 (MQ=255)

                                                                                    ggAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttg       >  1:3660853/1‑93 (MQ=255)

                                                                                     gagaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAttgtgttgt      <  1:6399374/93‑1 (MQ=255)

                                                                                       gaggCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATtgtgttgtgt    <  1:1109052/93‑1 (MQ=255)

ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT  >  NC_000913/2425683‑2425864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425680‑2425866

AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGG                                                                                               <  SRR4302536.2702941/94‑1 (MQ=60)

AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGG                                                                                               >  SRR4302536.876188/1‑94 (MQ=60)

  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             >  SRR4302536.2023785/1‑94 (MQ=60)

  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCC                                                                                             >  SRR4302536.5121312/1‑94 (MQ=60)

  CATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTCGAGAGGCC                                                                                             >  SRR4302536.6630021/1‑94 (MQ=60)

   ATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCG                                                                                            <  SRR4302536.860591/94‑1 (MQ=60)

      TTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACT                                                                                         <  SRR4302536.1968885/94‑1 (MQ=60)

        TCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAA                                                                                       <  SRR4302536.1133394/94‑1 (MQ=60)

        TCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAA                                                                                       >  SRR4302536.66555/1‑94 (MQ=60)

        TCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAA                                                                                       >  SRR4302536.2560357/1‑94 (MQ=60)

         CGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAAC                                                                                      >  SRR4302536.2188288/1‑94 (MQ=60)

          GATGAGAACGGTGCGTAGGTGGTATCGGTTCCNATNCGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     <  SRR4302536.5585495/94‑1 (MQ=60)

          GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     <  SRR4302536.3983856/94‑1 (MQ=60)

          GATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACA                                                                                     >  SRR4302536.731568/1‑94 (MQ=60)

           ATGAGAGCGGTGCGTAGGTGGTATCGGTTCCGATCCGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    <  SRR4302536.5213684/94‑1 (MQ=60)

           ATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAA                                                                                    <  SRR4302536.5693899/94‑1 (MQ=60)

            TGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAA                                                                                   >  SRR4302536.2298898/1‑94 (MQ=60)

             GAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAG                                                                                  <  SRR4302536.4002530/94‑1 (MQ=60)

             GAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAG                                                                                  <  SRR4302536.5972043/94‑1 (MQ=60)

              AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGA                                                                                 >  SRR4302536.1411254/1‑94 (MQ=60)

              AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGA                                                                                 >  SRR4302536.5432457/1‑94 (MQ=60)

              AGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGA                                                                                 >  SRR4302536.2767829/1‑94 (MQ=60)

               GTACGGTGGGTGGGTGGTATCGGTTCGGATAAGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGTAGAGGCCGACTAACAAAGAC                                                                                <  SRR4302536.6739648/94‑1 (MQ=60)

               GAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGAC                                                                                <  SRR4302536.1172135/94‑1 (MQ=60)

                AACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACA                                                                               >  SRR4302536.6105546/1‑94 (MQ=60)

                AACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACA                                                                               >  SRR4302536.2371042/1‑94 (MQ=60)

                 ACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAG                                                                              <  SRR4302536.5305679/94‑1 (MQ=60)

                    GTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGC                                                                           <  SRR4302536.4515524/94‑1 (MQ=60)

                    GTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGC                                                                           <  SRR4302536.6401738/94‑1 (MQ=60)

                    GTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGC                                                                           <  SRR4302536.5320384/94‑1 (MQ=60)

                     TGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCG                                                                          <  SRR4302536.2120204/94‑1 (MQ=60)

                       CGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAG                                                                        >  SRR4302536.3315733/1‑94 (MQ=60)

                       CGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAG                                                                        <  SRR4302536.2555113/94‑1 (MQ=60)

                         TAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAA                                                                      >  SRR4302536.2184312/1‑94 (MQ=60)

                         TAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAA                                                                      >  SRR4302536.4793292/1‑94 (MQ=60)

                          AGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAAT                                                                     >  SRR4302536.2175295/1‑94 (MQ=60)

                          AGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAAT                                                                     <  SRR4302536.6657007/94‑1 (MQ=60)

                           GGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATC                                                                    <  SRR4302536.1518516/94‑1 (MQ=60)

                           GGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATC                                                                    <  SRR4302536.3672306/94‑1 (MQ=60)

                            GTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCG                                                                   <  SRR4302536.498894/94‑1 (MQ=60)

                            GTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCG                                                                   >  SRR4302536.2643849/1‑94 (MQ=60)

                            GTGGAATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAACCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCTAGAATCG                                                                   <  SRR4302536.968145/94‑1 (MQ=60)

                              GGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGAC                                                                 >  SRR4302536.2104313/1‑94 (MQ=60)

                               GTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACT                                                                <  SRR4302536.3154657/94‑1 (MQ=60)

                                TATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTT                                                               >  SRR4302536.1188290/1‑94 (MQ=60)

                                  TCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             <  SRR4302536.1041671/94‑1 (MQ=60)

                                  TCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             <  SRR4302536.156722/94‑1 (MQ=60)

                                  TCGCTTCTGATACGTACGGCCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCT                                                             <  SRR4302536.1004824/94‑1 (MQ=60)

                                    GGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTC                                                           >  SRR4302536.2828174/1‑94 (MQ=60)

                                     GTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCA                                                          >  SRR4302536.840974/1‑94 (MQ=60)

                                         CGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACA                                                      >  SRR4302536.4683402/1‑94 (MQ=60)

                                         CGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACA                                                      >  SRR4302536.4902151/1‑94 (MQ=60)

                                           ATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATA                                                    >  SRR4302536.1614761/1‑94 (MQ=60)

                                           ATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATA                                                    >  SRR4302536.3408150/1‑94 (MQ=60)

                                              CGTCCCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACC                                                 <  SRR4302536.2657375/94‑1 (MQ=60)

                                              CGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACC                                                 <  SRR4302536.2006422/94‑1 (MQ=60)

                                               GTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCC                                                >  SRR4302536.498580/1‑94 (MQ=60)

                                                 ACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTC                                              >  SRR4302536.3707911/1‑94 (MQ=60)

                                                  CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             <  SRR4302536.37093/94‑1 (MQ=60)

                                                  CCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCA                                             <  SRR4302536.5642286/94‑1 (MQ=60)

                                                   CGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAA                                            <  SRR4302536.5207816/94‑1 (MQ=60)

                                                   CGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAA                                            >  SRR4302536.5807800/1‑94 (MQ=60)

                                                    GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           <  SRR4302536.6148385/94‑1 (MQ=60)

                                                    GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           <  SRR4302536.1687223/94‑1 (MQ=60)

                                                    GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           <  SRR4302536.533332/94‑1 (MQ=60)

                                                    GTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAG                                           <  SRR4302536.214485/94‑1 (MQ=60)

                                                     TCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302536.4943944/94‑1 (MQ=60)

                                                     TCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          >  SRR4302536.737211/1‑94 (MQ=60)

                                                     CCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGT                                          <  SRR4302536.5506608/94‑1 (MQ=60)

                                                      CTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTG                                         >  SRR4302536.5174562/1‑94 (MQ=60)

                                                        CCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAA                                       >  SRR4302536.6448618/1‑94 (MQ=60)

                                                          GGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAA                                     <  SRR4302536.1508228/94‑1 (MQ=60)

                                                           GTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAT                                    <  SRR4302536.4124584/94‑1 (MQ=60)

                                                           GTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAT                                    <  SRR4302536.1974384/94‑1 (MQ=60)

                                                           GTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAT                                    <  SRR4302536.1294744/94‑1 (MQ=60)

                                                           GTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAAT                                    >  SRR4302536.6371558/1‑94 (MQ=60)

                                                            TAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATG                                   >  SRR4302536.2171632/1‑94 (MQ=60)

                                                             AGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGG                                  >  SRR4302536.3692527/1‑94 (MQ=60)

                                                              GCGCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGC                                 <  SRR4302536.3161399/94‑1 (MQ=60)

                                                               agcgacgtagaTGGAAGCCTCTGTGGAGAGGCCGACTAACAGAGACAGAGCGAGAATCATCTTCATCATACATAACCCTCAAGTGAAAATGGCT                                <  SRR4302536.3169884/83‑1 (MQ=60)

                                                                GCCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTT                               <  SRR4302536.574073/94‑1 (MQ=60)

                                                                 CCGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTT                              <  SRR4302536.3461983/94‑1 (MQ=60)

                                                                  CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             >  SRR4302536.724567/1‑94 (MQ=60)

                                                                  CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             <  SRR4302536.6443426/94‑1 (MQ=60)

                                                                  CGCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTT                             >  SRR4302536.5591132/1‑94 (MQ=60)

                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            <  SRR4302536.2349630/94‑1 (MQ=60)

                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            >  SRR4302536.335698/1‑94 (MQ=60)

                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            >  SRR4302536.4995975/1‑94 (MQ=60)

                                                                   GCATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTT                            >  SRR4302536.5796215/1‑94 (MQ=60)

                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           <  SRR4302536.1258545/94‑1 (MQ=60)

                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           >  SRR4302536.4007659/1‑94 (MQ=60)

                                                                    CATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTT                           >  SRR4302536.5319034/1‑94 (MQ=60)

                                                                     ATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAGCAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTA                          <  SRR4302536.5099258/94‑1 (MQ=60)

                                                                     ATAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTA                          >  SRR4302536.4536611/1‑94 (MQ=60)

                                                                      TAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAT                         <  SRR4302536.3055012/94‑1 (MQ=60)

                                                                      TAGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTAT                         <  SRR4302536.3096328/94‑1 (MQ=60)

                                                                       AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        >  SRR4302536.283352/1‑94 (MQ=60)

                                                                       AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        <  SRR4302536.6143256/94‑1 (MQ=60)

                                                                       AGCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATG                        >  SRR4302536.2720117/1‑94 (MQ=60)

                                                                        GCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGT                       <  SRR4302536.5422500/94‑1 (MQ=60)

                                                                        GCTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGT                       >  SRR4302536.361089/1‑94 (MQ=60)

                                                                         CTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTT                      <  SRR4302536.4838982/94‑1 (MQ=60)

                                                                         CTGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTT                      <  SRR4302536.824053/94‑1 (MQ=60)

                                                                          TGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTG                     <  SRR4302536.3662768/94‑1 (MQ=60)

                                                                          TGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTG                     <  SRR4302536.6372714/94‑1 (MQ=60)

                                                                          TGGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTG                     >  SRR4302536.4302480/1‑94 (MQ=60)

                                                                           GGAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGT                    <  SRR4302536.1209767/94‑1 (MQ=60)

                                                                            GAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTG                   <  SRR4302536.1304115/94‑1 (MQ=60)

                                                                            GAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTG                   >  SRR4302536.3405060/1‑94 (MQ=60)

                                                                            GAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTG                   >  SRR4302536.6222538/1‑94 (MQ=60)

                                                                            GAAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTG                   <  SRR4302536.593331/94‑1 (MQ=60)

                                                                             AAGCCGCTGTGGAGGGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCGTACATAACCCTCAAGGGAAAATGGCTTTTTTATGTTGggg                  >  SRR4302536.4722352/1‑91 (MQ=60)

                                                                             AAGCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGT                  >  SRR4302536.3608435/1‑94 (MQ=60)

                                                                               GCCGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTAT                <  SRR4302536.4411764/94‑1 (MQ=60)

                                                                                 CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              >  SRR4302536.1684669/1‑94 (MQ=60)

                                                                                 CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              <  SRR4302536.6848594/94‑1 (MQ=60)

                                                                                 CGCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTG              <  SRR4302536.2061180/94‑1 (MQ=60)

                                                                                 CGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCCAGTGAAAATGGCTTTTTTATGTTGTGTATTG              >  SRR4302536.3295903/1‑94 (MQ=60)

                                                                                  GCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGT             <  SRR4302536.6005062/94‑1 (MQ=60)

                                                                                  GCTGTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGT             <  SRR4302536.1989506/94‑1 (MQ=60)

                                                                                     GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          <  SRR4302536.6643438/94‑1 (MQ=60)

                                                                                     GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          <  SRR4302536.988418/94‑1 (MQ=60)

                                                                                     GTGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTT          <  SRR4302536.486209/94‑1 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         <  SRR4302536.5904631/94‑1 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         >  SRR4302536.6741377/1‑94 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         <  SRR4302536.5686879/94‑1 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         <  SRR4302536.4797790/94‑1 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         >  SRR4302536.3770293/1‑94 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTG         >  SRR4302536.4087469/1‑94 (MQ=60)

                                                                                      TGGAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGGGTATTGTGTTG         >  SRR4302536.1832738/1‑94 (MQ=60)

                                                                                        GAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTG       <  SRR4302536.6590365/94‑1 (MQ=60)

                                                                                        GAGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTG       <  SRR4302536.6403363/94‑1 (MQ=60)

                                                                                         AGAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGT      <  SRR4302536.6499653/94‑1 (MQ=60)

                                                                                          GAGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTG     <  SRR4302536.1142324/94‑1 (MQ=60)

                                                                                           AGGCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT    >  SRR4302536.2429929/1‑94 (MQ=60)

                                                                                           AGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGT    >  SRR4302536.1511449/1‑94 (MQ=60)

                                                                                             GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  SRR4302536.4270242/1‑94 (MQ=60)

                                                                                             GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  <  SRR4302536.5823800/94‑1 (MQ=60)

                                                                                             GCCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  <  SRR4302536.903421/94‑1 (MQ=60)

AGCATCTTTCGATGAGAACGGTGCGTAGGTGGTATCGGTTCCGATACGTACCGTCTCCGGTAGCGCCGCATAGCTGGAAGCCGCTGTGGAGAGACCGACTAACAAAGACAGAGCGAGAATCGACTTCTTCATACATAACCCTCAAGTGAAAATGGCTTTTTTATGTTGTGTATTGTGTTGTGTGTTT  >  NC_000913/2425680‑2425866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`