Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,403,945 T→C V131A (GTA→GCA)  accC → acetyl CoA carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,403,9450TC98.9% 587.6 / ‑3.1 188V131A (GTA→GCA) accCacetyl CoA carboxylase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (96/90);  total (97/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 199 total aligned reads displayed.

ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403854‑3404034
                                                                                           |                                                                                         
aTCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAATTATTCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTa                                                                                          >  2:4362465/1‑93 (MQ=255)
  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAcc                                                                                        >  1:2263482/1‑93 (MQ=255)
  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCAcc                                                                                        <  1:2631273/93‑1 (MQ=255)
  cTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCCCGGCGATGAAAAAAGCGGGAGTCCCTTGCGCAcc                                                                                        >  2:1615936/1‑93 (MQ=255)
   ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       <  2:3871621/93‑1 (MQ=255)
   ttCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCg                                                                                       >  1:2782633/1‑93 (MQ=255)
      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  2:3411954/1‑93 (MQ=255)
      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  1:1649828/1‑93 (MQ=255)
      aTTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGt                                                                                    >  2:2229475/1‑93 (MQ=255)
         ggCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCt                                                                                 <  2:2712758/93‑1 (MQ=255)
           cccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGa                                                                               <  2:120375/93‑1 (MQ=255)
            ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              <  1:3303929/93‑1 (MQ=255)
            ccGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAc                                                                              >  1:3092607/1‑93 (MQ=255)
             cGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACg                                                                             >  2:1504127/1‑93 (MQ=255)
              gAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACgg                                                                            >  1:2965959/1‑93 (MQ=255)
               aaaGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGc                                                                           >  2:2912205/1‑93 (MQ=255)
                 aGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGccc                                                                         >  2:4543505/1‑93 (MQ=255)
                  gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  1:2458667/1‑93 (MQ=255)
                  gCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCg                                                                        >  1:3410227/1‑93 (MQ=255)
                   cAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGc                                                                       <  1:3699935/93‑1 (MQ=255)
                    aGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCt                                                                      <  2:2522911/93‑1 (MQ=255)
                    aGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCt                                                                      >  1:3154648/1‑93 (MQ=255)
                     gAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTg                                                                     >  2:4481249/1‑93 (MQ=255)
                      aaaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTgg                                                                    >  1:2014363/1‑93 (MQ=255)
                       aaCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTggg                                                                   >  1:1679666/1‑93 (MQ=255)
                        aCCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGc                                                                  >  1:402897/1‑93 (MQ=255)
                         ccATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcg                                                                 >  2:4606590/1‑93 (MQ=255)
                          cATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcga                                                                >  2:4038460/1‑93 (MQ=255)
                           aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               >  1:4676722/1‑93 (MQ=255)
                           aTTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgac                                                               <  2:1558124/93‑1 (MQ=255)
                             ttgCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgacga                                                             <  1:3032832/91‑1 (MQ=255)
                             tCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGcgacga                                                             <  1:2435000/93‑1 (MQ=255)
                              cGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGat                                                            >  1:1987948/1‑93 (MQ=255)
                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           >  2:2336931/1‑93 (MQ=255)
                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  1:4502980/93‑1 (MQ=255)
                               gCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGata                                                           <  2:2416221/93‑1 (MQ=255)
                                 cTGATTGGCGACAAATTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  1:322121/93‑1 (MQ=255)
                                 cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  1:3617640/93‑1 (MQ=255)
                                 cTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATg                                                         <  2:2324135/93‑1 (MQ=255)
                                  tGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATgg                                                        <  2:2965638/93‑1 (MQ=255)
                                   gATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGa                                                       <  1:2911976/93‑1 (MQ=255)
                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      <  1:4430750/93‑1 (MQ=255)
                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      >  2:3696207/1‑93 (MQ=255)
                                    aTGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAt                                                      >  1:2669958/1‑93 (MQ=255)
                                     tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  1:4746119/93‑1 (MQ=255)
                                     tGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATa                                                     <  2:4728029/93‑1 (MQ=255)
                                       ggCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaa                                                   <  2:4088851/93‑1 (MQ=255)
                                         cGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATaaaaa                                                 <  2:3764071/93‑1 (MQ=255)
                                          gACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAc                                                >  1:3897865/1‑93 (MQ=255)
                                           aCAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAcc                                               >  2:3038154/1‑93 (MQ=255)
                                            cAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCg                                              <  2:1210399/93‑1 (MQ=255)
                                             aaaGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGt                                             <  2:202207/93‑1 (MQ=255)
                                                  aTCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAt                                        <  1:3460083/93‑1 (MQ=255)
                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:116277/93‑1 (MQ=255)
                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:3827665/93‑1 (MQ=255)
                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       <  1:2241289/93‑1 (MQ=255)
                                                   tCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCAtt                                       >  1:484466/1‑93 (MQ=255)
                                                    ccGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTg                                      >  2:395312/1‑93 (MQ=255)
                                                     cGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGc                                     <  1:2265748/93‑1 (MQ=255)
                                                       cAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTa                                   >  1:3633940/1‑93 (MQ=255)
                                                         aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 >  2:2620062/1‑93 (MQ=255)
                                                         aTCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTCGGCGACGATATGGATAAAAACCGTGCCATTGCTaaa                                 >  1:477278/1‑93 (MQ=255)
                                                            gcggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGc                              >  2:704054/1‑93 (MQ=255)
                                                              ggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  2:3240867/93‑1 (MQ=255)
                                                              ggcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCAt                            <  2:2080079/93‑1 (MQ=255)
                                                               gcgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCGTTGCTAAACGCAtt                           >  2:2178270/1‑93 (MQ=255)
                                                                cgATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTg                          >  1:671974/1‑93 (MQ=255)
                                                                 gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         <  2:3571819/93‑1 (MQ=255)
                                                                 gATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTgg                         <  2:3084429/93‑1 (MQ=255)
                                                                  aTGAAAAAAGCGGGCGTCCCTTGCTCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGTTATAAACCGTGCCATTGCTAAACTCATTGGt                        >  2:4196345/1‑93 (MQ=255)
                                                                  aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        >  2:3593812/1‑93 (MQ=255)
                                                                  aTGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGt                        >  2:4549942/1‑93 (MQ=255)
                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  2:491762/1‑93 (MQ=255)
                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  1:353140/1‑93 (MQ=255)
                                                                   tGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       >  1:875232/1‑93 (MQ=255)
                                                                   tGAAAAAAGCGGGCGTCCCTGGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGtt                       <  1:3528413/93‑1 (MQ=255)
                                                                     aaaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATAGATAAAAACCGAGCCAGTGCTAAACGCATTGGGTAt                     >  2:886240/1‑93 (MQ=255)
                                                                      aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    <  1:1234635/93‑1 (MQ=255)
                                                                      aaaaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCCCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATc                    >  2:2472495/1‑93 (MQ=255)
                                                                       aaaaGCGGGCGTCCCTTGCGCGCCGGGTTCTGACCGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATcc                   <  1:1333075/93‑1 (MQ=255)
                                                                        aaaGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCg                  >  1:912881/1‑93 (MQ=255)
                                                                         aaGCGCGCCTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCgg                 <  1:3882564/93‑1 (MQ=255)
                                                                             gggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAt             <  2:1923087/93‑1 (MQ=255)
                                                                              ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            >  2:800530/1‑93 (MQ=255)
                                                                              ggCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGAtt            >  1:1495634/1‑93 (MQ=255)
                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  1:4151905/93‑1 (MQ=255)
                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  1:1264824/93‑1 (MQ=255)
                                                                                cGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAt          <  2:107248/93‑1 (MQ=255)
                                                                                  tCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCa        >  1:386311/1‑93 (MQ=255)
                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      >  2:656260/1‑93 (MQ=255)
                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      <  2:3351798/93‑1 (MQ=255)
                                                                                    ccTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCaaa      <  2:277781/93‑1 (MQ=255)
                                                                                     ctggcgcACCGTGTGCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  1:1137385/90‑1 (MQ=255)
                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  2:1679587/1‑93 (MQ=255)
                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     <  2:4523288/93‑1 (MQ=255)
                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  2:3933092/1‑93 (MQ=255)
                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  1:4717779/1‑93 (MQ=255)
                                                                                     cTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAg     >  1:968193/1‑93 (MQ=255)
                                                                                      ttGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGc    <  2:2844040/93‑1 (MQ=255)
                                                                                       tGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGcc   <  2:3465332/93‑1 (MQ=255)
                                                                                           |                                                                                         
ATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403854‑3404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403852‑3404034
                                                                                             |                                                                                         
TTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGC                                                                                           >  SRR4302544.4743240/1‑94 (MQ=60)
   TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACC                                                                                        >  SRR4302544.2284772/1‑94 (MQ=60)
   TCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACC                                                                                        >  SRR4302544.2284771/1‑94 (MQ=60)
    CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAGAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                       <  SRR4302544.2656634/94‑1 (MQ=60)
    CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                       <  SRR4302544.2656496/94‑1 (MQ=60)
    CTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCG                                                                                       >  SRR4302544.2809413/1‑94 (MQ=60)
     TTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGG                                                                                      >  SRR4302544.2423671/1‑94 (MQ=60)
       CATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGT                                                                                    >  SRR4302544.1666104/1‑94 (MQ=60)
        ATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTT                                                                                   >  SRR4302544.1459033/1‑94 (MQ=60)
           GGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTG                                                                                <  SRR4302544.3358775/94‑1 (MQ=60)
             CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGGGTCCCTTGCGCACCGGGTTCTGAG                                                                              >  SRR4302544.2780360/1‑94 (MQ=60)
             CCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGAC                                                                              >  SRR4302544.3122065/1‑94 (MQ=60)
              CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                             <  SRR4302544.3335400/94‑1 (MQ=60)
              CCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACG                                                                             <  SRR4302544.1248695/94‑1 (MQ=60)
               CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGG                                                                            >  SRR4302544.3241823/1‑94 (MQ=60)
               CGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGG                                                                            >  SRR4302544.2994414/1‑94 (MQ=60)
                 AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                          >  SRR4302544.3529677/1‑94 (MQ=60)
                 AAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCC                                                                          >  SRR4302544.1331654/1‑94 (MQ=60)
                  AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCC                                                                         >  SRR4302544.3662090/1‑94 (MQ=60)
                  AAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCC                                                                         <  SRR4302544.4271025/94‑1 (MQ=60)
                   AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCG                                                                        >  SRR4302544.3442827/1‑94 (MQ=60)
                   AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCG                                                                        <  SRR4302544.3596471/94‑1 (MQ=60)
                   AGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCG                                                                        >  SRR4302544.2482126/1‑94 (MQ=60)
                     CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                      <  SRR4302544.3736771/94‑1 (MQ=60)
                     CAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCT                                                                      >  SRR4302544.3184656/1‑94 (MQ=60)
                      AGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTG                                                                     >  SRR4302544.3876729/1‑94 (MQ=60)
                       GAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGG                                                                    >  SRR4302544.2033937/1‑94 (MQ=60)
                        AAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGG                                                                   <  SRR4302544.2470625/94‑1 (MQ=60)
                        AAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGG                                                                   >  SRR4302544.1696209/1‑94 (MQ=60)
                        AAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGG                                                                   <  SRR4302544.2270832/94‑1 (MQ=60)
                         AACCATTCGGCTGATGGGCGACGAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                  <  SRR4302544.3085791/94‑1 (MQ=60)
                         AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                  >  SRR4302544.406992/1‑94 (MQ=60)
                         AACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGC                                                                  >  SRR4302544.406991/1‑94 (MQ=60)
                          ACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCG                                                                 <  SRR4302544.924288/94‑1 (MQ=60)
                          ACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCG                                                                 <  SRR4302544.4756688/94‑1 (MQ=60)
                            CATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGAC                                                               >  SRR4302544.4722946/1‑94 (MQ=60)
                               TTGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGAT                                                            <  SRR4302544.3061887/94‑1 (MQ=60)
                               TCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGAT                                                            >  SRR4302544.2007244/1‑94 (MQ=60)
                               TCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGAT                                                            <  SRR4302544.381861/94‑1 (MQ=60)
                               TCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGAT                                                            <  SRR4302544.2458212/94‑1 (MQ=60)
                                 GCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATAT                                                          <  SRR4302544.4548152/94‑1 (MQ=60)
                                   CTGATTGGCGACAAATTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                        <  SRR4302544.325465/94‑1 (MQ=60)
                                   CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                        >  SRR4302544.3180107/1‑94 (MQ=60)
                                   CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                        <  SRR4302544.2581269/94‑1 (MQ=60)
                                   CTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGG                                                        <  SRR4302544.3653526/94‑1 (MQ=60)
                                     GATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAT                                                      <  SRR4302544.2939987/94‑1 (MQ=60)
                                     GATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGAT                                                      >  SRR4302544.2695551/1‑94 (MQ=60)
                                      ATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATA                                                     <  SRR4302544.4475455/94‑1 (MQ=60)
                                      ATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATA                                                     <  SRR4302544.3483978/94‑1 (MQ=60)
                                       TGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAA                                                    <  SRR4302544.4792881/94‑1 (MQ=60)
                                        GGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAA                                                   >  SRR4302544.887960/1‑94 (MQ=60)
                                           CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                >  SRR4302544.3936582/1‑94 (MQ=60)
                                           CGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAAC                                                <  SRR4302544.2993743/94‑1 (MQ=60)
                                                    tgCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                       <  SRR4302544.1182492/92‑1 (MQ=60)
                                                    ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                       <  SRR4302544.3493107/94‑1 (MQ=60)
                                                    ATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATT                                       >  SRR4302544.489208/1‑94 (MQ=60)
                                                     TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                      <  SRR4302544.117337/94‑1 (MQ=60)
                                                     TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                      <  SRR4302544.3865623/94‑1 (MQ=60)
                                                     TCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTG                                      <  SRR4302544.2262376/94‑1 (MQ=60)
                                                      CCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGC                                     >  SRR4302544.4793810/1‑94 (MQ=60)
                                                       CGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCT                                    <  SRR4302544.2287068/94‑1 (MQ=60)
                                                        GCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTA                                   >  SRR4302544.3670175/1‑94 (MQ=60)
                                                          AATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTCGGCGACGATATGGATAAAAACCGTGCCATTGCTAAA                                 >  SRR4302544.481953/1‑94 (MQ=60)
                                                            TCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACG                               <  SRR4302544.864171/94‑1 (MQ=60)
                                                             CGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGC                              <  SRR4302544.2076758/94‑1 (MQ=60)
                                                              GCGGCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCA                             >  SRR4302544.97773/1‑94 (MQ=60)
                                                                 GCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTG                          >  SRR4302544.678614/1‑94 (MQ=60)
                                                                 GCGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTG                          <  SRR4302544.172375/94‑1 (MQ=60)
                                                                  CGATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGG                         >  SRR4302544.4327271/1‑94 (MQ=60)
                                                                   GATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGT                        <  SRR4302544.3291930/94‑1 (MQ=60)
                                                                    ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                       >  SRR4302544.883915/1‑94 (MQ=60)
                                                                    ATGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTT                       >  SRR4302544.356755/1‑94 (MQ=60)
                                                                     TGAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTA                      <  SRR4302544.3562143/94‑1 (MQ=60)
                                                                     TGAAAAAAGCGGGCGTCCCTGGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTA                      <  SRR4302544.3562142/94‑1 (MQ=60)
                                                                      GAAAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTAT                     >  SRR4302544.477187/1‑94 (MQ=60)
                                                                      GAAAAAAGCGGGCGTCCCTTACGCACCGGGTTCTGACGGCCAGCTGGGCGACGATATGGATAAAAGCCGTGCCATTGCTAAACGCATTGGTTAT                     <  SRR4302544.1271428/94‑1 (MQ=60)
                                                                        AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                   <  SRR4302544.1246845/94‑1 (MQ=60)
                                                                        AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                   <  SRR4302544.155187/94‑1 (MQ=60)
                                                                        AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                   <  SRR4302544.2837647/94‑1 (MQ=60)
                                                                        AAAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCC                   <  SRR4302544.3904310/94‑1 (MQ=60)
                                                                         AAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                  <  SRR4302544.3709082/94‑1 (MQ=60)
                                                                         AAAAGCGGGCGTCCCTTGCGCGCCGGGTTCTGACCGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                  <  SRR4302544.1346202/94‑1 (MQ=60)
                                                                         AAAAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCG                  >  SRR4302544.921909/1‑94 (MQ=60)
                                                                           aaggtggggtcttTTGCGCACCGGGTTCTGACGGCCCGCTGGGGGACGATATGGCTAAAAACCGTGCCATTGCTTAACGCATTGGTTATCCGGT                <  SRR4302544.1259876/81‑1 (MQ=60)
                                                                           AAGCGGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGT                <  SRR4302544.3921128/94‑1 (MQ=60)
                                                                           AAGCGCGCCTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGT                <  SRR4302544.3921127/94‑1 (MQ=60)
                                                                               GGGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATT            >  SRR4302544.1510426/1‑94 (MQ=60)
                                                                                GGCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTA           <  SRR4302544.462215/94‑1 (MQ=60)
                                                                                 GCGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTAT          >  SRR4302544.3033953/1‑94 (MQ=60)
                                                                                  CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATC         <  SRR4302544.1277270/94‑1 (MQ=60)
                                                                                  CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATC         >  SRR4302544.586724/1‑94 (MQ=60)
                                                                                  CGTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATC         <  SRR4302544.4193022/94‑1 (MQ=60)
                                                                                   GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA        >  SRR4302544.390239/1‑94 (MQ=60)
                                                                                   GTCCCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCA        >  SRR4302544.2009934/1‑94 (MQ=60)
                                                                                      CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG     >  SRR4302544.4764379/1‑94 (MQ=60)
                                                                                      CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG     >  SRR4302544.452736/1‑94 (MQ=60)
                                                                                      CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG     <  SRR4302544.1684096/94‑1 (MQ=60)
                                                                                      CCTTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAG     >  SRR4302544.977688/1‑94 (MQ=60)
                                                                                        TTGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCC   >  SRR4302544.488969/1‑94 (MQ=60)
                                                                                         TGCGCACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  <  SRR4302544.4137644/94‑1 (MQ=60)
                                                                                             |                                                                                         
TTATCTTCATTGGCCCGAAAGCAGAAACCATTCGCCTGATGGGCGACAAAGTATCCGCAATCGCGGCGATGAAAAAAGCGGGCGTCCCTTGCGTACCGGGTTCTGACGGCCCGCTGGGCGACGATATGGATAAAAACCGTGCCATTGCTAAACGCATTGGTTATCCGGTGATTATCAAAGCCT  >  NC_000913/3403852‑3404034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑