Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,410,192 T→C intergenic (+18/‑66) b3262 → / → yhdU putative methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,1920TC98.8% 799.5 / ‑1.2 248intergenic (+18/‑66)b3262/yhdUputative methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base C (155/90);  total (155/93)
Fisher's exact test for biased strand distribution p-value = 5.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 262 total aligned reads displayed.

AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410100‑3410281
                                                                                            |                                                                                         
aaaaaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGt                                                                                           <  1:3106984/93‑1 (MQ=255)
  aaaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGctt                                                                                         <  1:2315199/93‑1 (MQ=255)
   aaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGcttt                                                                                        >  2:3774419/1‑93 (MQ=255)
    aaagaaagACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTc                                                                                       <  2:848283/93‑1 (MQ=255)
     aagaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCa                                                                                      >  1:1813300/1‑93 (MQ=255)
      agaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAg                                                                                     >  1:1316544/1‑93 (MQ=255)
       gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    >  1:3633791/1‑93 (MQ=255)
          agaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAATCCTGGCTTTCAGatta                                                                                 >  2:1356087/1‑93 (MQ=255)
           gaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattat                                                                                >  2:2786405/1‑93 (MQ=255)
            aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACACAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               >  2:3696282/1‑93 (MQ=255)
            aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               <  2:4710303/93‑1 (MQ=255)
              cGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCa                                                                             <  1:580199/93‑1 (MQ=255)
               ggCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAt                                                                            <  2:4242420/93‑1 (MQ=255)
               ggCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAt                                                                            >  2:3533729/1‑93 (MQ=255)
                gCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAtt                                                                           <  1:1965397/93‑1 (MQ=255)
                gCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAtt                                                                           >  2:4548404/1‑93 (MQ=255)
                gCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAtt                                                                           >  2:3939086/1‑93 (MQ=255)
                  aaCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTc                                                                         >  1:455331/1‑93 (MQ=255)
                   aCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCg                                                                        >  1:677704/1‑93 (MQ=255)
                   aCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCg                                                                        >  2:1179587/1‑93 (MQ=255)
                       gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  1:4350880/93‑1 (MQ=255)
                         cAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGtata                                                                  <  1:2422012/93‑1 (MQ=255)
                          aaaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTatat                                                                 >  2:1049268/1‑93 (MQ=255)
                          aaaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTatat                                                                 >  2:310317/1‑93 (MQ=255)
                           aaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                <  2:1558083/93‑1 (MQ=255)
                           aaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                <  1:750828/93‑1 (MQ=255)
                            aaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAttt                                                               >  1:2107767/1‑93 (MQ=255)
                             aCGAAGCCGTTAAAGGGAAGTTGACCCGGATCTCATTAC‑AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTcc                                                             <  1:548165/93‑1 (MQ=255)
                              cGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTcc                                                             <  2:2102702/93‑1 (MQ=255)
                               gAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCt                                                            <  2:3869694/93‑1 (MQ=255)
                               gAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCt                                                            >  2:889366/1‑93 (MQ=255)
                                aaGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTg                                                           <  2:2910496/93‑1 (MQ=255)
                                 aGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGc                                                          <  1:1025041/93‑1 (MQ=255)
                                 aGCCGGTCAAGCGAATTTGACCCCGATCTCATTACAAAGTATAGGATGTGTAAGCATGGCTTTCAGATTATTCATTTCGTGTATATTTTCTGc                                                          >  2:3287581/1‑93 (MQ=255)
                                    cGGTTAATCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAg                                                       >  1:1254421/1‑93 (MQ=255)
                                      gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     >  2:3195651/1‑93 (MQ=255)
                                      gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     >  1:400692/1‑93 (MQ=255)
                                      gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     <  1:1551166/93‑1 (MQ=255)
                                      gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAACCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     >  2:2935059/1‑93 (MQ=255)
                                        tAAGCGAAGTTGACCGCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACtt                                                   <  1:3775419/93‑1 (MQ=255)
                                        tAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACtt                                                   <  1:3160927/93‑1 (MQ=255)
                                        tAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACtt                                                   >  1:522712/1‑93 (MQ=255)
                                         aaGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  >  1:1375338/1‑93 (MQ=255)
                                          aGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTgg                                                 <  2:1925382/93‑1 (MQ=255)
                                           gCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGt                                                <  2:3996178/93‑1 (MQ=255)
                                           gCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGt                                                >  2:1371211/1‑93 (MQ=255)
                                             gAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTa                                              >  1:4275777/1‑93 (MQ=255)
                                             gAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATATCGTGTATATTTCCTGCCAGACGTGGTTa                                              >  1:279290/1‑93 (MQ=255)
                                                gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           >  2:3903378/1‑93 (MQ=255)
                                                 ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          <  2:53215/93‑1 (MQ=255)
                                                    aCCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGc                                       >  1:4107660/1‑93 (MQ=255)
                                                      cccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcac                                     <  2:961864/93‑1 (MQ=255)
                                                       ccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcaca                                    <  1:1009691/93‑1 (MQ=255)
                                                       ccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcaca                                    >  2:739305/1‑93 (MQ=255)
                                                        cGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAg                                   <  1:1918915/93‑1 (MQ=255)
                                                         gATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAgg                                  >  1:1108931/1‑93 (MQ=255)
                                                          aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 <  1:784541/93‑1 (MQ=255)
                                                          aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 <  1:2784703/93‑1 (MQ=255)
                                                          aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 >  1:2993888/1‑93 (MQ=255)
                                                          aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 >  2:3601054/1‑93 (MQ=255)
                                                          aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 >  2:305510/1‑93 (MQ=255)
                                                           tctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCt                                >  1:809922/1‑93 (MQ=255)
                                                           tctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCt                                >  2:4673753/1‑93 (MQ=255)
                                                            ttcATTACAAGGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               <  1:324265/92‑1 (MQ=255)
                                                            ctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               >  1:4193162/1‑93 (MQ=255)
                                                            ctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               >  2:4148652/1‑93 (MQ=255)
                                                               aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            <  1:3969809/93‑1 (MQ=255)
                                                               aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            <  2:184819/93‑1 (MQ=255)
                                                                  aCAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTaa                         >  1:3477527/1‑93 (MQ=255)
                                                                   cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        >  2:4523054/1‑93 (MQ=255)
                                                                   cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        <  2:662056/93‑1 (MQ=255)
                                                                   cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        <  1:2534254/93‑1 (MQ=255)
                                                                   cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        <  2:552452/93‑1 (MQ=255)
                                                                    aaaGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATg                       >  2:1015562/1‑93 (MQ=255)
                                                                     aaGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGa                      >  1:3930589/1‑93 (MQ=255)
                                                                       gTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAtt                    <  2:2417736/93‑1 (MQ=255)
                                                                        tAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTc                   >  2:478226/1‑93 (MQ=255)
                                                                         aaaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCg                  >  1:3489863/1‑93 (MQ=255)
                                                                         aaaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCg                  >  1:1657479/1‑93 (MQ=255)
                                                                          aaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGc                 >  2:601586/1‑93 (MQ=255)
                                                                            ggATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCaa               <  2:3497544/93‑1 (MQ=255)
                                                                            ggATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCaa               <  1:3045211/93‑1 (MQ=255)
                                                                            ggATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCaa               >  2:2523858/1‑93 (MQ=255)
                                                                             gATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAg              <  1:1126469/93‑1 (MQ=255)
                                                                               tgtgTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTa            >  1:2713750/1‑93 (MQ=255)
                                                                               tgtgTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTa            >  1:3743817/1‑93 (MQ=255)
                                                                                gtgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAt           <  2:2248025/93‑1 (MQ=255)
                                                                                 tgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAtt          <  1:411918/93‑1 (MQ=255)
                                                                                 tgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAtt          >  2:44078/1‑93 (MQ=255)
                                                                                  gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         <  1:3458818/93‑1 (MQ=255)
                                                                                  gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         <  2:2739219/93‑1 (MQ=255)
                                                                                  gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         >  2:1433924/1‑93 (MQ=255)
                                                                                     aGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtg      >  1:2263891/1‑93 (MQ=255)
                                                                                     aGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtg      <  2:2849034/93‑1 (MQ=255)
                                                                                      gCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtgg     >  2:2195002/1‑93 (MQ=255)
                                                                                        cTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   >  1:2434368/1‑93 (MQ=255)
                                                                                        cTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   >  1:3589668/1‑93 (MQ=255)
                                                                                        cTGGCTGTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   <  2:331393/93‑1 (MQ=255)
                                                                                         tGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCtc  >  1:4701008/1‑93 (MQ=255)
                                                                                         tGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCtc  >  2:2658291/1‑93 (MQ=255)
                                                                                            |                                                                                         
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410100‑3410281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410100‑3410285
                                                                                            |                                                                                             
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTT                                                                                              <  SRR4302544.3136560/94‑1 (MQ=60)
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              >  SRR4302544.2335492/1‑94 (MQ=60)
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              >  SRR4302544.1216552/1‑94 (MQ=60)
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              >  SRR4302544.3532669/1‑94 (MQ=60)
 AAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTT                                                                                             >  SRR4302544.1479169/1‑94 (MQ=60)
  AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTT                                                                                            >  SRR4302544.3794730/1‑94 (MQ=60)
  AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTT                                                                                            <  SRR4302544.2337112/94‑1 (MQ=60)
    AAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCA                                                                                          >  SRR4302544.1831007/1‑94 (MQ=60)
     AAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAG                                                                                         <  SRR4302544.3094892/94‑1 (MQ=60)
     AAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAG                                                                                         >  SRR4302544.1329490/1‑94 (MQ=60)
      AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGA                                                                                        >  SRR4302544.3670025/1‑94 (MQ=60)
         AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302544.3827662/1‑94 (MQ=60)
         AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302544.4286368/1‑94 (MQ=60)
         AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302544.1797074/1‑94 (MQ=60)
          AGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTAT                                                                                    >  SRR4302544.1099033/1‑94 (MQ=60)
          AGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTAT                                                                                    >  SRR4302544.2077634/1‑94 (MQ=60)
            ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  <  SRR4302544.4002008/94‑1 (MQ=60)
             CCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTTCAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCA                                                                                 >  SRR4302544.709859/1‑94 (MQ=60)
              CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAT                                                                                <  SRR4302544.585864/94‑1 (MQ=60)
                GCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTT                                                                              <  SRR4302544.1984483/94‑1 (MQ=60)
                 CAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTC                                                                             >  SRR4302544.459828/1‑94 (MQ=60)
                 CAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTC                                                                             >  SRR4302544.2021356/1‑94 (MQ=60)
                 CAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTC                                                                             <  SRR4302544.1882232/94‑1 (MQ=60)
                  AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            >  SRR4302544.684422/1‑94 (MQ=60)
                  AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            <  SRR4302544.4291606/94‑1 (MQ=60)
                  AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            <  SRR4302544.3742996/94‑1 (MQ=60)
                    CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          >  SRR4302544.367470/1‑94 (MQ=60)
                    CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          >  SRR4302544.80927/1‑94 (MQ=60)
                    CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          >  SRR4302544.1168786/1‑94 (MQ=60)
                      TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        >  SRR4302544.3256500/1‑94 (MQ=60)
                      TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        >  SRR4302544.1029073/1‑94 (MQ=60)
                       GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       <  SRR4302544.573847/94‑1 (MQ=60)
                       GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       <  SRR4302544.4395085/94‑1 (MQ=60)
                        TCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATA                                                                      <  SRR4302544.3644704/94‑1 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     >  SRR4302544.4462648/1‑94 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302544.2655651/94‑1 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302544.2445075/94‑1 (MQ=60)
                          AAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATT                                                                    >  SRR4302544.2158517/1‑94 (MQ=60)
                          AAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATT                                                                    >  SRR4302544.2452220/1‑94 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   <  SRR4302544.758162/94‑1 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302544.2128044/1‑94 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302544.2514045/1‑94 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   <  SRR4302544.3512785/94‑1 (MQ=60)
                            AACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTC                                                                  <  SRR4302544.595782/94‑1 (MQ=60)
                             ACGAAGCCGTTAAAGGGAAGTTGACCCGGATCTCATTAC‑AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCT                                                                <  SRR4302544.553540/94‑1 (MQ=60)
                             ACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCC                                                                 <  SRR4302544.442363/94‑1 (MQ=60)
                              CGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCT                                                                <  SRR4302544.1314457/94‑1 (MQ=60)
                               GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTG                                                               >  SRR4302544.620089/1‑94 (MQ=60)
                               GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCGGGATTTCAGATTATTAATTTCGTGTATATTTtctc                                                               >  SRR4302544.1349873/1‑90 (MQ=60)
                                AAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGC                                                              >  SRR4302544.2309024/1‑94 (MQ=60)
                                 AGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCC                                                             <  SRR4302544.4601358/94‑1 (MQ=60)
                                 AGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCC                                                             <  SRR4302544.1035274/94‑1 (MQ=60)
                                   CCGGTTAATCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAG                                                           >  SRR4302544.1266762/1‑94 (MQ=60)
                                    CGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGA                                                          >  SRR4302544.3190135/1‑94 (MQ=60)
                                     GGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAC                                                         >  SRR4302544.404764/1‑94 (MQ=60)
                                      GTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACT                                                        <  SRR4302544.1566615/94‑1 (MQ=60)
                                       TTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTT                                                       >  SRR4302544.527884/1‑94 (MQ=60)
                                       TTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTT                                                       <  SRR4302544.4093530/94‑1 (MQ=60)
                                       TTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTT                                                       >  SRR4302544.527885/1‑94 (MQ=60)
                                       GTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTT                                                       <  SRR4302544.1129338/94‑1 (MQ=60)
                                        TAAGCGAAGTTGACCGCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTG                                                      <  SRR4302544.3812836/94‑1 (MQ=60)
                                        TAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTG                                                      <  SRR4302544.3190988/94‑1 (MQ=60)
                                        TAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTG                                                      >  SRR4302544.1389045/1‑94 (MQ=60)
                                         AAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGG                                                     <  SRR4302544.1908721/94‑1 (MQ=60)
                                           GCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTT                                                   >  SRR4302544.77633/1‑94 (MQ=60)
                                           GCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTT                                                   >  SRR4302544.4369016/1‑94 (MQ=60)
                                            CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  >  SRR4302544.282207/1‑94 (MQ=60)
                                            CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  >  SRR4302544.4319433/1‑94 (MQ=60)
                                            CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  >  SRR4302544.2404709/1‑94 (MQ=60)
                                            CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATATCGTGTATATTTCCTGCCAGACGTGGTTA                                                  >  SRR4302544.282208/1‑94 (MQ=60)
                                             GAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAA                                                 >  SRR4302544.3635205/1‑94 (MQ=60)
                                             GAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAA                                                 >  SRR4302544.545425/1‑94 (MQ=60)
                                              AAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTTTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAA                                                <  SRR4302544.4153864/94‑1 (MQ=60)
                                                GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACA                                              >  SRR4302544.1398614/1‑94 (MQ=60)
                                                   GACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGC                                           >  SRR4302544.4148499/1‑94 (MQ=60)
                                                    ACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCA                                          >  SRR4302544.2639312/1‑94 (MQ=60)
                                                    ACCACGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCA                                          <  SRR4302544.2951234/94‑1 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        <  SRR4302544.4070496/94‑1 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        >  SRR4302544.1318892/1‑94 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        >  SRR4302544.1087050/1‑94 (MQ=60)
                                                       CCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTCTATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       >  SRR4302544.4622808/1‑94 (MQ=60)
                                                       CCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAG                                       <  SRR4302544.1019744/94‑1 (MQ=60)
                                                        CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      <  SRR4302544.1937456/94‑1 (MQ=60)
                                                        CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      >  SRR4302544.1119988/1‑94 (MQ=60)
                                                         GATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGC                                     >  SRR4302544.3022592/1‑94 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    <  SRR4302544.2811499/94‑1 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    <  SRR4302544.792134/94‑1 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302544.353548/1‑94 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302544.3262874/1‑94 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302544.817819/1‑94 (MQ=60)
                                                           TCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTC                                   >  SRR4302544.4234695/1‑94 (MQ=60)
                                                           TCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTC                                   <  SRR4302544.692577/94‑1 (MQ=60)
                                                            TTCATTACAAGGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  <  SRR4302544.327634/94‑1 (MQ=60)
                                                             TCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAG                                 >  SRR4302544.4021517/1‑94 (MQ=60)
                                                              CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302544.3843841/1‑94 (MQ=60)
                                                              CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302544.2231744/1‑94 (MQ=60)
                                                              CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAGTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302544.817351/1‑94 (MQ=60)
                                                               ATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGT                               <  SRR4302544.2955819/94‑1 (MQ=60)
                                                               ATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGT                               <  SRR4302544.4009296/94‑1 (MQ=60)
                                                                 TACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAA                             >  SRR4302544.3510686/1‑94 (MQ=60)
                                                                  ACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAT                            <  SRR4302544.2431820/94‑1 (MQ=60)
                                                                   CAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATG                           <  SRR4302544.2558597/94‑1 (MQ=60)
                                                                    AAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGA                          >  SRR4302544.3969628/1‑94 (MQ=60)
                                                                     AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAT                         <  SRR4302544.1635252/94‑1 (MQ=60)
                                                                      AGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATT                        >  SRR4302544.1956956/1‑94 (MQ=60)
                                                                       GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       >  SRR4302544.1817689/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302544.1673812/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302544.3394305/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302544.3523127/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302544.3523128/1‑94 (MQ=60)
                                                                         AAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGC                     >  SRR4302544.948628/1‑94 (MQ=60)
                                                                            GGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAG                  <  SRR4302544.3074329/94‑1 (MQ=60)
                                                                             GATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGT                 <  SRR4302544.1137697/94‑1 (MQ=60)
                                                                              ATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTA                >  SRR4302544.2739858/1‑94 (MQ=60)
                                                                              ATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTA                <  SRR4302544.4004999/94‑1 (MQ=60)
                                                                              ATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTA                >  SRR4302544.3781012/1‑94 (MQ=60)
                                                                              ATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGCGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTA                >  SRR4302544.2739859/1‑94 (MQ=60)
                                                                                GTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATT              <  SRR4302544.506727/94‑1 (MQ=60)
                                                                                 TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             >  SRR4302544.4182460/1‑94 (MQ=60)
                                                                                 TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             <  SRR4302544.416119/94‑1 (MQ=60)
                                                                                  GTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGG            <  SRR4302544.3491830/94‑1 (MQ=60)
                                                                                   TAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGT           <  SRR4302544.3086756/94‑1 (MQ=60)
                                                                                   TAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGT           >  SRR4302544.2508493/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302544.2285186/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302544.2817230/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302544.4200802/1‑94 (MQ=60)
                                                                                     AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         <  SRR4302544.1225344/94‑1 (MQ=60)
                                                                                      GCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGC        <  SRR4302544.961728/94‑1 (MQ=60)
                                                                                       CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       >  SRR4302544.3624141/1‑94 (MQ=60)
                                                                                       CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       >  SRR4302544.3501697/1‑94 (MQ=60)
                                                                                       CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       >  SRR4302544.3050861/1‑94 (MQ=60)
                                                                                       CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       >  SRR4302544.2457572/1‑94 (MQ=60)
                                                                                        CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302544.4747477/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGCTTCGCAAGTATTtgtagctct     >  SRR4302544.128928/1‑85 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302544.3994770/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302544.3721288/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     <  SRR4302544.3422372/94‑1 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302544.128927/1‑94 (MQ=60)
                                                                                           GCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTC   >  SRR4302544.4167792/1‑94 (MQ=60)
                                                                                           GCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTC   <  SRR4302544.1209939/94‑1 (MQ=60)
                                                                                            CTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  SRR4302544.327420/1‑94 (MQ=60)
                                                                                            |                                                                                             
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410100‑3410285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑