Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,410,192 T→C intergenic (+18/‑66) b3262 → / → yhdU putative methyltransferase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,410,1920TC99.6% 910.2 / ‑5.8 278intergenic (+18/‑66)b3262/yhdUputative methyltransferase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (152/125);  total (153/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410102‑3410281
                                                                                          |                                                                                         
aaaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGctt                                                                                         >  1:323965/1‑93 (MQ=255)
 aaaaGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGCTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGcttt                                                                                        >  2:4694196/1‑93 (MQ=255)
   aagaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCa                                                                                      >  2:293949/1‑93 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    >  2:5455984/1‑93 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    >  2:3766606/1‑93 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    <  2:2730372/93‑1 (MQ=255)
     gaaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGa                                                                                    >  1:3614280/1‑93 (MQ=255)
      aaagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGat                                                                                   <  1:1127980/93‑1 (MQ=255)
       aagaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGatt                                                                                  >  2:5023750/1‑93 (MQ=255)
        agaCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGatta                                                                                 >  2:629062/1‑93 (MQ=255)
          aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               >  2:5822463/1‑93 (MQ=255)
          aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               <  1:5764341/93‑1 (MQ=255)
          aCCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGattatt                                                                               <  1:4851135/93‑1 (MQ=255)
           ccGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTc                                                                              <  2:4560821/93‑1 (MQ=255)
              gCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAtt                                                                           >  2:3242400/1‑93 (MQ=255)
               cAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAttt                                                                          >  2:535210/1‑93 (MQ=255)
                aaCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTc                                                                         >  2:4133482/1‑93 (MQ=255)
                 aCCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCg                                                                        <  1:3105845/93‑1 (MQ=255)
                   cTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgtg                                                                      >  2:5983200/1‑93 (MQ=255)
                    tGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgtgt                                                                     >  1:2342580/1‑93 (MQ=255)
                    tGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCgtgt                                                                     >  1:6513938/1‑93 (MQ=255)
                     gTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGta                                                                    <  2:6145446/93‑1 (MQ=255)
                      tCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGtat                                                                   >  1:133618/1‑93 (MQ=255)
                       cAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGtata                                                                  <  1:5531945/93‑1 (MQ=255)
                        aaaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTatat                                                                 <  1:4991585/93‑1 (MQ=255)
                         aaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                <  1:730278/93‑1 (MQ=255)
                         aaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                >  2:6329462/1‑93 (MQ=255)
                         aaaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAtt                                                                >  2:3854978/1‑93 (MQ=255)
                          aaCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAttt                                                               <  1:5128374/93‑1 (MQ=255)
                           aCGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTc                                                              <  2:4300022/93‑1 (MQ=255)
                              aaGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTg                                                           >  1:1961957/1‑93 (MQ=255)
                                 ccGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCa                                                        <  2:4371251/93‑1 (MQ=255)
                                 ccGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCa                                                        >  2:1051143/1‑93 (MQ=255)
                                 ccGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCa                                                        <  2:6438613/93‑1 (MQ=255)
                                  cGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAg                                                       <  2:6627244/93‑1 (MQ=255)
                                   ggTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGa                                                      >  2:5585080/1‑93 (MQ=255)
                                    gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     >  2:402179/1‑93 (MQ=255)
                                    gTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     <  1:6432271/93‑1 (MQ=255)
                                    gTTAAGCGAAGTTAACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAc                                                     >  1:3858381/1‑93 (MQ=255)
                                     ttAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACt                                                    <  2:460831/93‑1 (MQ=255)
                                      tAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACtt                                                   <  1:2105525/93‑1 (MQ=255)
                                       aaGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  >  2:5245263/1‑93 (MQ=255)
                                       aaGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  >  2:1371796/1‑93 (MQ=255)
                                       aaGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTg                                                  >  1:431486/1‑93 (MQ=255)
                                        aGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTgg                                                 >  2:4970611/1‑93 (MQ=255)
                                        aGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTgg                                                 >  2:2578348/1‑93 (MQ=255)
                                            aaGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTaa                                             <  1:779756/93‑1 (MQ=255)
                                              gTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAAc                                           <  1:5444580/93‑1 (MQ=255)
                                               ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          <  2:3576849/93‑1 (MQ=255)
                                               ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          <  1:2999213/93‑1 (MQ=255)
                                               ttGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACa                                          >  2:2031472/1‑93 (MQ=255)
                                                tGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACAt                                         >  1:1853577/1‑93 (MQ=255)
                                                 gACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATg                                        <  2:4856502/93‑1 (MQ=255)
                                                   ccccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAATATGca                                      >  2:2111198/1‑93 (MQ=255)
                                                    cccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcac                                     <  1:2586151/93‑1 (MQ=255)
                                                    cccGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGcac                                     >  2:331434/1‑93 (MQ=255)
                                                      cGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAg                                   <  1:2688619/93‑1 (MQ=255)
                                                       gATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAgg                                  <  2:6385691/93‑1 (MQ=255)
                                                       gATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAgg                                  <  1:4594634/93‑1 (MQ=255)
                                                       gATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAgg                                  >  1:3413838/1‑93 (MQ=255)
                                                        aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 >  1:280029/1‑93 (MQ=255)
                                                        aTCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGc                                 <  1:3906942/93‑1 (MQ=255)
                                                         tctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCt                                >  2:1552924/1‑93 (MQ=255)
                                                         tctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCt                                >  1:6335817/1‑93 (MQ=255)
                                                          ctcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTc                               <  1:42410/93‑1 (MQ=255)
                                                           tcATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCa                              >  1:1317349/1‑93 (MQ=255)
                                                            cATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAg                             >  1:1737617/1‑93 (MQ=255)
                                                             aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            >  1:4031621/1‑93 (MQ=255)
                                                             aTTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAgg                            >  2:6733184/1‑93 (MQ=255)
                                                              ttACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGt                           >  1:1556714/1‑93 (MQ=255)
                                                               tACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTa                          >  2:1658326/1‑93 (MQ=255)
                                                                aCAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTaa                         >  1:216930/1‑93 (MQ=255)
                                                                 cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        >  2:3919354/1‑93 (MQ=255)
                                                                 cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        >  1:5342931/1‑93 (MQ=255)
                                                                 cAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAt                        >  1:1040195/1‑93 (MQ=255)
                                                                    aGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAt                     <  1:1755108/93‑1 (MQ=255)
                                                                     gTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAtt                    <  2:3367913/93‑1 (MQ=255)
                                                                     gTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAtt                    >  2:1943486/1‑93 (MQ=255)
                                                                     gTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAtt                    >  1:945730/1‑93 (MQ=255)
                                                                      tAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTc                   >  1:1481200/1‑93 (MQ=255)
                                                                       aaaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCg                  >  1:2479053/1‑93 (MQ=255)
                                                                       aaaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCg                  <  1:5618614/93‑1 (MQ=255)
                                                                       aaaGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTAAAACATGCACAGGCTCAGGTAATGATTCg                  <  2:2519819/93‑1 (MQ=255)
                                                                         aGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCa                >  2:1745366/1‑93 (MQ=255)
                                                                           gATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAg              >  2:2061456/1‑93 (MQ=255)
                                                                             tgtgTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTa            >  2:3531137/1‑93 (MQ=255)
                                                                             tgtgTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTa            <  1:4896044/93‑1 (MQ=255)
                                                                              gtgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAt           >  1:115984/1‑93 (MQ=255)
                                                                               tgtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAtt          <  2:3458685/93‑1 (MQ=255)
                                                                                gtAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtg         >  2:1184133/1‑93 (MQ=255)
                                                                                 tAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtgg        <  2:6550896/93‑1 (MQ=255)
                                                                                  aaGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggt       <  2:5060813/93‑1 (MQ=255)
                                                                                  aaGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggt       <  2:3108459/93‑1 (MQ=255)
                                                                                  aaGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggt       <  1:6707234/93‑1 (MQ=255)
                                                                                  aaGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggt       >  2:985975/1‑93 (MQ=255)
                                                                                   aGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtg      >  1:5890593/1‑93 (MQ=255)
                                                                                   aGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtg      >  1:3678866/1‑93 (MQ=255)
                                                                                    gCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATtggtgg     >  1:6267504/1‑93 (MQ=255)
                                                                                      cTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCt   >  2:5622430/1‑93 (MQ=255)
                                                                                       tGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCtc  <  1:331434/93‑1 (MQ=255)
                                                                                          |                                                                                         
AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC  >  NC_000913/3410102‑3410281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410100‑3410285
                                                                                            |                                                                                             
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              >  SRR4302536.6611822/1‑94 (MQ=60)
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCT                                                                                              <  SRR4302536.3255966/94‑1 (MQ=60)
 AAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTT                                                                                             <  SRR4302536.1578052/94‑1 (MQ=60)
 AAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTT                                                                                             <  SRR4302536.3401381/94‑1 (MQ=60)
 AAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTT                                                                                             >  SRR4302536.332559/1‑94 (MQ=60)
  AAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTT                                                                                            >  SRR4302536.3258782/1‑94 (MQ=60)
   AAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTC                                                                                           <  SRR4302536.5380843/94‑1 (MQ=60)
   AAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTC                                                                                           <  SRR4302536.6007887/94‑1 (MQ=60)
    AAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCA                                                                                          >  SRR4302536.3419766/1‑94 (MQ=60)
      AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGA                                                                                        >  SRR4302536.3722332/1‑94 (MQ=60)
      AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGA                                                                                        >  SRR4302536.4483213/1‑94 (MQ=60)
      AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGA                                                                                        >  SRR4302536.5922948/1‑94 (MQ=60)
      AGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAACCTGGCTTTCAGT                                                                                        >  SRR4302536.1805889/1‑94 (MQ=60)
       GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       >  SRR4302536.4039117/1‑94 (MQ=60)
       GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       <  SRR4302536.1817347/94‑1 (MQ=60)
       GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       <  SRR4302536.6512879/94‑1 (MQ=60)
       GAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGAT                                                                                       <  SRR4302536.3857957/94‑1 (MQ=60)
        AAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACACAGTAAAGGATGTGTAAGCCTGGCTTTCAGATT                                                                                      >  SRR4302536.2744707/1‑94 (MQ=60)
        AAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATT                                                                                      <  SRR4302536.6660813/94‑1 (MQ=60)
        AAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATT                                                                                      <  SRR4302536.1161883/94‑1 (MQ=60)
         AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302536.2563938/1‑94 (MQ=60)
         AAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTA                                                                                     >  SRR4302536.3768198/1‑94 (MQ=60)
          AGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTAT                                                                                    <  SRR4302536.5691415/94‑1 (MQ=60)
           GACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATT                                                                                   <  SRR4302536.5591967/94‑1 (MQ=60)
            ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  <  SRR4302536.4997110/94‑1 (MQ=60)
            ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  <  SRR4302536.6894550/94‑1 (MQ=60)
            ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  <  SRR4302536.1855307/94‑1 (MQ=60)
            ACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTC                                                                                  <  SRR4302536.5935818/94‑1 (MQ=60)
             CCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGGTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCA                                                                                 >  SRR4302536.3310788/1‑94 (MQ=60)
              CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAT                                                                                >  SRR4302536.899950/1‑94 (MQ=60)
              CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAT                                                                                >  SRR4302536.794728/1‑94 (MQ=60)
              CGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCAT                                                                                >  SRR4302536.661952/1‑94 (MQ=60)
               GGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATT                                                                               >  SRR4302536.4373585/1‑94 (MQ=60)
                GCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTT                                                                              <  SRR4302536.3390355/94‑1 (MQ=60)
                 CAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTC                                                                             >  SRR4302536.5390238/1‑94 (MQ=60)
                  AACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACTCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCG                                                                            >  SRR4302536.2419466/1‑94 (MQ=60)
                   ACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGT                                                                           <  SRR4302536.3196521/94‑1 (MQ=60)
                    CCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTG                                                                          <  SRR4302536.5235640/94‑1 (MQ=60)
                     CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         <  SRR4302536.1244598/94‑1 (MQ=60)
                     CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302536.400209/1‑94 (MQ=60)
                     CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302536.6708035/1‑94 (MQ=60)
                     CTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGT                                                                         >  SRR4302536.2413629/1‑94 (MQ=60)
                      TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        <  SRR4302536.9874/94‑1 (MQ=60)
                      TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        <  SRR4302536.1224950/94‑1 (MQ=60)
                      TGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTA                                                                        <  SRR4302536.6492000/94‑1 (MQ=60)
                       GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       <  SRR4302536.2362192/94‑1 (MQ=60)
                       GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       <  SRR4302536.1368209/94‑1 (MQ=60)
                       GTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTAT                                                                       >  SRR4302536.137129/1‑94 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302536.2721919/94‑1 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302536.2197348/94‑1 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     <  SRR4302536.5696854/94‑1 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     >  SRR4302536.1381705/1‑94 (MQ=60)
                         CAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATAT                                                                     >  SRR4302536.1567977/1‑94 (MQ=60)
                          AAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATT                                                                    <  SRR4302536.5141404/94‑1 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   <  SRR4302536.751676/94‑1 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302536.4820940/1‑94 (MQ=60)
                           AAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTT                                                                   >  SRR4302536.4386432/1‑94 (MQ=60)
                            AACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTC                                                                  >  SRR4302536.5182272/1‑94 (MQ=60)
                            AACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTC                                                                  <  SRR4302536.5281571/94‑1 (MQ=60)
                             ACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCC                                                                 <  SRR4302536.3677627/94‑1 (MQ=60)
                              CGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCT                                                                <  SRR4302536.714279/94‑1 (MQ=60)
                               GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTG                                                               >  SRR4302536.2020950/1‑94 (MQ=60)
                               GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTG                                                               >  SRR4302536.3883216/1‑94 (MQ=60)
                               GAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTG                                                               <  SRR4302536.5055950/94‑1 (MQ=60)
                                AAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGC                                                              <  SRR4302536.1704762/94‑1 (MQ=60)
                                AAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGC                                                              >  SRR4302536.4037587/1‑94 (MQ=60)
                                AAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGC                                                              >  SRR4302536.2220617/1‑94 (MQ=60)
                                  GCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCA                                                            >  SRR4302536.774491/1‑94 (MQ=60)
                                   CCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAG                                                           <  SRR4302536.1374803/94‑1 (MQ=60)
                                     GGTTAAGCGAAGTTAACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGAC                                                         >  SRR4302536.3973208/1‑94 (MQ=60)
                                      GTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACT                                                        <  SRR4302536.6624518/94‑1 (MQ=60)
                                       TTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTT                                                       <  SRR4302536.1795664/94‑1 (MQ=60)
                                        TAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTG                                                      <  SRR4302536.2168815/94‑1 (MQ=60)
                                        TAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTG                                                      >  SRR4302536.442838/1‑94 (MQ=60)
                                         AAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGG                                                     <  SRR4302536.2829206/94‑1 (MQ=60)
                                            CGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTA                                                  <  SRR4302536.5541673/94‑1 (MQ=60)
                                              AAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAA                                                <  SRR4302536.802603/94‑1 (MQ=60)
                                                GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACA                                              <  SRR4302536.5607442/94‑1 (MQ=60)
                                                GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACA                                              <  SRR4302536.5057221/94‑1 (MQ=60)
                                                GTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTGCGTGTATATTTCCTGCTAGACTTGGTTAAACA                                              <  SRR4302536.927376/94‑1 (MQ=60)
                                                 TTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACAT                                             >  SRR4302536.1909436/1‑94 (MQ=60)
                                                 TTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACAT                                             <  SRR4302536.3087391/94‑1 (MQ=60)
                                                  TGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATG                                            >  SRR4302536.644481/1‑94 (MQ=60)
                                                   GACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGC                                           >  SRR4302536.5655722/1‑94 (MQ=60)
                                                     CCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCAC                                         <  SRR4302536.428880/94‑1 (MQ=60)
                                                     CCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCAC                                         >  SRR4302536.4650428/1‑94 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        >  SRR4302536.3116725/1‑94 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        <  SRR4302536.2663848/94‑1 (MQ=60)
                                                      CCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACA                                        >  SRR4302536.1965952/1‑94 (MQ=60)
                                                        CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      <  SRR4302536.2768893/94‑1 (MQ=60)
                                                        CGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGG                                      >  SRR4302536.3515415/1‑94 (MQ=60)
                                                         GATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGC                                     <  SRR4302536.4732568/94‑1 (MQ=60)
                                                         GATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGC                                     >  SRR4302536.287478/1‑94 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302536.1191219/1‑94 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    <  SRR4302536.4023061/94‑1 (MQ=60)
                                                          ATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCT                                    >  SRR4302536.6524506/1‑94 (MQ=60)
                                                            CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  <  SRR4302536.6035517/94‑1 (MQ=60)
                                                            CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  <  SRR4302536.43587/94‑1 (MQ=60)
                                                            CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  >  SRR4302536.2072597/1‑94 (MQ=60)
                                                            CTCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCA                                  >  SRR4302536.1356677/1‑94 (MQ=60)
                                                             TCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAG                                 <  SRR4302536.5005357/94‑1 (MQ=60)
                                                             TCATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAG                                 >  SRR4302536.1787734/1‑94 (MQ=60)
                                                              CATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGG                                >  SRR4302536.4151193/1‑94 (MQ=60)
                                                               ATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGT                               >  SRR4302536.2008319/1‑94 (MQ=60)
                                                               ATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGT                               >  SRR4302536.1482650/1‑94 (MQ=60)
                                                               ATTACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGT                               >  SRR4302536.1602435/1‑94 (MQ=60)
                                                                 TACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAA                             >  SRR4302536.222685/1‑94 (MQ=60)
                                                                  ACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAT                            >  SRR4302536.5502497/1‑94 (MQ=60)
                                                                  ACAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAAT                            >  SRR4302536.1071102/1‑94 (MQ=60)
                                                                   CAAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATG                           <  SRR4302536.3719153/94‑1 (MQ=60)
                                                                    AAAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGA                          >  SRR4302536.109398/1‑94 (MQ=60)
                                                                     AAGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGAT                         <  SRR4302536.2916800/94‑1 (MQ=60)
                                                                      AGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATT                        <  SRR4302536.1807245/94‑1 (MQ=60)
                                                                      AGTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATT                        >  SRR4302536.973658/1‑94 (MQ=60)
                                                                       GTAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTC                       >  SRR4302536.1524961/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302536.2553871/1‑94 (MQ=60)
                                                                        TAAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCG                      >  SRR4302536.3465545/1‑94 (MQ=60)
                                                                         AAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGC                     >  SRR4302536.5763214/1‑94 (MQ=60)
                                                                         AAAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGC                     <  SRR4302536.5785511/94‑1 (MQ=60)
                                                                          AAGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCA                    >  SRR4302536.6771873/1‑94 (MQ=60)
                                                                           AGGATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCGA                   >  SRR4302536.4351937/1‑94 (MQ=60)
                                                                           AGGATGTGTAAACCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAA                   >  SRR4302536.374644/1‑94 (MQ=60)
                                                                              ATGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTA                >  SRR4302536.5868848/1‑94 (MQ=60)
                                                                               TGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAT               >  SRR4302536.119051/1‑94 (MQ=60)
                                                                               TGTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTAT               <  SRR4302536.5043291/94‑1 (MQ=60)
                                                                                GTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATT              <  SRR4302536.3393622/94‑1 (MQ=60)
                                                                                GTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATT              <  SRR4302536.1535984/94‑1 (MQ=60)
                                                                                GTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATT              <  SRR4302536.2500682/94‑1 (MQ=60)
                                                                                GTGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATT              <  SRR4302536.6201530/94‑1 (MQ=60)
                                                                                 TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             <  SRR4302536.2910162/94‑1 (MQ=60)
                                                                                 TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             >  SRR4302536.722569/1‑94 (MQ=60)
                                                                                 TGTAAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTG             >  SRR4302536.2639256/1‑94 (MQ=60)
                                                                                  GTAAGCCTGGATTTCTGAGTATTAATTTCATGTATATTTACTACCAGCCTTGGTTAAACATGCACTGGCTCAGGTAATGATTCGCATGTATTat            >  SRR4302536.4561770/1‑92 (MQ=38)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302536.6066011/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302536.2807163/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302536.3788838/1‑94 (MQ=60)
                                                                                    AAGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          <  SRR4302536.6906404/94‑1 (MQ=60)
                                                                                    AAGCCTGGCTCTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTG          >  SRR4302536.2105573/1‑94 (MQ=60)
                                                                                     AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         >  SRR4302536.6454227/1‑94 (MQ=60)
                                                                                     AGCCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGG         >  SRR4302536.1184653/1‑94 (MQ=60)
                                                                                       CCTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCT       >  SRR4302536.4880958/1‑94 (MQ=60)
                                                                                        CTGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTC      >  SRR4302536.6586243/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302536.5598562/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     <  SRR4302536.340231/94‑1 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302536.5950198/1‑94 (MQ=60)
                                                                                         TGGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCG     >  SRR4302536.5153457/1‑94 (MQ=60)
                                                                                          GGCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGT    <  SRR4302536.5026531/94‑1 (MQ=60)
                                                                                           GCTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTC   <  SRR4302536.5255229/94‑1 (MQ=60)
                                                                                            CTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  <  SRR4302536.1394282/94‑1 (MQ=60)
                                                                                            CTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  SRR4302536.6140038/1‑94 (MQ=60)
                                                                                            |                                                                                             
AAAAAAAGAAAGACCGGCAACCTGTCAAAACGAAGCCGGTTAAGCGAAGTTGACCCCGATCTCATTACAAAGTAAAGGATGTGTAAGCCTGGTTTTCAGATTATTCATTTCGTGTATATTTCCTGCCAGACTTGGTTAAACATGCACAGGCTCAGGTAATGATTCGCAAGTATTGGTGGCTCGTCG  >  NC_000913/3410100‑3410285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑