Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,418,840 +G coding (1070/1089 nt) b3269 → putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,418,8371.G98.4% 403.2 / ‑1.4 127coding (1067/1089 nt)b3269putative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (63/62);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 153 total aligned reads displayed.

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924
                                                                                              |                                                                                         
gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑c                                                                                            >  1:2890048/1‑93 (MQ=255)
gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑c                                                                                            >  2:3735426/1‑93 (MQ=255)
gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑c                                                                                            <  2:3023509/93‑1 (MQ=255)
gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑c                                                                                            >  1:2212157/1‑93 (MQ=255)
gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑c                                                                                            >  1:3196051/1‑93 (MQ=255)
 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑g                                                                                         >  2:1381586/1‑93 (MQ=255)
  aTCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑gg                                                                                        <  2:3459653/93‑1 (MQ=255)
   tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATCTGGTTTCGCTGTTTGCCGGCACCGTCCTGAATCAGA‑C‑‑ggg                                                                                       >  1:494405/1‑93 (MQ=255)
   tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggg                                                                                       >  2:3721389/1‑93 (MQ=255)
   tctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggg                                                                                       >  1:2913488/1‑93 (MQ=255)
     tgaaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATTGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggggc                                                                                     >  2:2711714/3‑91 (MQ=255)
      cAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggggca                                                                                    <  2:2547508/93‑4 (MQ=255)
      cAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggggca                                                                                    >  2:2394732/1‑90 (MQ=255)
       aaCATCAGCAAATACTCCTCTCTTGCCGCCGCTATTGCATATCCCGATATAGTTTCGCTGCTTGCCGGCACCGTGCTGAATCAGA‑C‑‑ggggcga                                                                                   >  2:1495114/1‑89 (MQ=255)
            cGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCAt                                                                              <  2:497900/93‑1 (MQ=255)
             gTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATc                                                                             <  1:3217726/93‑1 (MQ=255)
               cAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCga                                                                           <  1:3811925/93‑1 (MQ=255)
               cAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCga                                                                           <  1:1138732/93‑1 (MQ=255)
               cAAAAACTCCTCTCTTGCCGCAGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCga                                                                           <  1:490443/93‑1 (MQ=255)
                aaaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCgag                                                                          >  1:1309000/1‑93 (MQ=255)
                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCTGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCgaga                                                                         <  1:3272246/93‑1 (MQ=255)
                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATTGTTTCGCTGTTTGCCGGCGCCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCgaga                                                                         <  1:157079/93‑1 (MQ=255)
                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCgaga                                                                         <  1:2271061/93‑1 (MQ=255)
                  aaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGAc                                                                        <  2:3865124/93‑1 (MQ=255)
                    aCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGa                                                                      <  2:1295807/93‑1 (MQ=255)
                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGAt                                                                     >  2:3844532/1‑93 (MQ=255)
                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTACCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGAt                                                                     >  2:466533/1‑93 (MQ=255)
                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATa                                                                    >  1:469702/1‑93 (MQ=255)
                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAg                                                                   >  1:1981725/1‑93 (MQ=255)
                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGcc                                                                 >  2:2082959/1‑93 (MQ=255)
                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGcc                                                                 >  2:2256939/1‑93 (MQ=255)
                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGcc                                                                 >  2:2256940/1‑93 (MQ=255)
                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGcc                                                                 >  1:4631578/1‑93 (MQ=255)
                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGACa                                                                >  1:976883/1‑93 (MQ=255)
                          catttGCCACCGCTCTTGCGTATCCGGATTGGGTTTCGCTGTGTGCCGGCCCCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCa                                                                <  2:3879806/90‑1 (MQ=255)
                           tctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCAt                                                               >  2:168661/1‑93 (MQ=255)
                           tctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCAt                                                               <  2:4238999/93‑1 (MQ=255)
                            ctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATg                                                              >  2:870624/1‑93 (MQ=255)
                             ttGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATgc                                                             >  2:2854561/1‑92 (MQ=255)
                             ttGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGa                                                             >  1:60114/1‑93 (MQ=255)
                             ttGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGa                                                             >  1:3945230/1‑93 (MQ=255)
                                ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCa                                                          <  1:4626555/93‑1 (MQ=255)
                                ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCa                                                          >  1:3285070/1‑93 (MQ=255)
                                 cgccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCAt                                                         >  1:3480964/1‑93 (MQ=255)
                                  gccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATg                                                        <  1:3620979/93‑1 (MQ=255)
                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       >  1:3401054/1‑93 (MQ=255)
                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       >  1:3018144/1‑93 (MQ=255)
                                    cgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      >  1:18649/1‑93 (MQ=255)
                                    cgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      <  2:2507016/93‑1 (MQ=255)
                                     gcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CACGCCATCGAGACGATAGACATGACCATGTCg                                                     >  2:2313514/1‑93 (MQ=255)
                                     gcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCg                                                     >  2:2353256/1‑93 (MQ=255)
                                       tATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                   >  1:175478/1‑93 (MQ=255)
                                       tATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                   >  1:1063939/1‑93 (MQ=255)
                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  >  1:2546393/1‑93 (MQ=255)
                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTAGGTc                                                  >  2:624459/1‑93 (MQ=255)
                                          tGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTa                                                <  2:4212698/93‑1 (MQ=255)
                                           ggCTATCCCGATATGTTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAt                                               <  2:74194/93‑1 (MQ=255)
                                           ggCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAt                                               <  2:1488496/93‑1 (MQ=255)
                                            gCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATc                                              <  2:4433319/93‑1 (MQ=255)
                                            gCTATCCCGATATGGTTTCGCTGTTGGCCGGCACCGTGCTGAATCAGA‑C‑‑GGGCCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATc                                              <  2:1896797/93‑1 (MQ=255)
                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             <  1:2369165/93‑1 (MQ=255)
                                              tATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTg                                            >  1:534788/1‑93 (MQ=255)
                                               aTCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGa                                           <  2:1749426/93‑1 (MQ=255)
                                                 cccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                         <  2:359520/93‑1 (MQ=255)
                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                        >  1:2536390/1‑93 (MQ=255)
                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGAACATGTCTGACTATCTGATTa                                        >  2:2178126/1‑93 (MQ=255)
                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGACATGACCAAGTCGGTCTATCTGATTa                                        >  2:4403775/1‑93 (MQ=255)
                                                   cGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAt                                       >  2:578675/1‑93 (MQ=255)
                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      >  2:3281186/1‑93 (MQ=255)
                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CGGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCa                                     <  2:1691956/93‑1 (MQ=255)
                                                        tGGTTTCGCTGTTTGCCGGCCCCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  <  2:3368905/93‑1 (MQ=255)
                                                        tGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  >  1:3091666/1‑93 (MQ=255)
                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                <  1:1239908/93‑1 (MQ=255)
                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                >  1:2817112/1‑93 (MQ=255)
                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                <  2:3219891/93‑1 (MQ=255)
                                                           tttCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGa                               <  1:315666/93‑1 (MQ=255)
                                                           tttCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGa                               >  1:1908481/1‑93 (MQ=255)
                                                           tttCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGa                               >  1:4051228/1‑93 (MQ=255)
                                                             tCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACt                             <  2:3318587/93‑1 (MQ=255)
                                                             tCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACt                             <  1:2467529/93‑1 (MQ=255)
                                                             tCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACt                             <  2:1789097/93‑1 (MQ=255)
                                                              cGCTGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTa                            <  1:4561915/93‑1 (MQ=255)
                                                              cGCTGTTTGCCGGCACCGTGCTAAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTa                            <  2:3534759/93‑1 (MQ=255)
                                                                 tGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  2:2022985/93‑1 (MQ=255)
                                                                 tGTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  1:790269/93‑1 (MQ=255)
                                                                  gTTTGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        <  1:4519676/93‑1 (MQ=255)
                                                                    ttGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgc                      >  1:2843424/1‑93 (MQ=255)
                                                                     tGCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgct                     <  2:4478389/93‑1 (MQ=255)
                                                                      gCCGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctg                    >  2:3618255/1‑93 (MQ=255)
                                                                        cGGCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgct                  <  2:212233/93‑1 (MQ=255)
                                                                          gCACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                >  2:3512637/1‑93 (MQ=255)
                                                                           cACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgat               <  1:3383722/93‑1 (MQ=255)
                                                                           cACCGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgat               >  1:1657863/1‑93 (MQ=255)
                                                                             ccGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatga             >  2:4735068/1‑93 (MQ=255)
                                                                              cGTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGaa            <  1:3145839/93‑1 (MQ=255)
                                                                               gTGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAat           >  2:550897/1‑93 (MQ=255)
                                                                                tGCTGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAata          <  1:400627/93‑1 (MQ=255)
                                                                                  cTGAATCAGAGG‑‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATc        <  2:1984147/93‑1 (MQ=255)
                                                                                   tGAATCAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCt       <  1:389146/93‑1 (MQ=255)
                                                                                        cAGA‑CG‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAc  <  1:2994935/93‑1 (MQ=255)
                                                                                              |                                                                                         
GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGA‑C‑‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT  >  NC_000913/3418747‑3418932
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ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATCTGGTTTCGCTGTTTGCCGGCACCGTCCTGAATCAGAC‑GGG                                                                                              >  SRR4302544.499267/1‑94 (MQ=60)
ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG                                                                                              >  SRR4302544.499266/1‑94 (MQ=60)
ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG                                                                                              >  SRR4302544.2941511/1‑94 (MQ=60)
  CTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGGA                                                                                            >  SRR4302544.189186/1‑94 (MQ=60)
     AACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAG                                                                                          <  SRR4302544.379027/94‑1 (MQ=60)
           GTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCG                                                                                    <  SRR4302544.3248424/94‑1 (MQ=60)
            TCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGA                                                                                   >  SRR4302544.1560784/1‑94 (MQ=60)
             CAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAG                                                                                  <  SRR4302544.1150054/94‑1 (MQ=60)
             CAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAG                                                                                  <  SRR4302544.3849705/94‑1 (MQ=60)
             CAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAG                                                                                  >  SRR4302544.1321868/1‑94 (MQ=60)
             CAAAAACTCCTCTCTTGCCGCAGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAG                                                                                  <  SRR4302544.495259/94‑1 (MQ=60)
               AAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCTGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGAC                                                                                <  SRR4302544.3303451/94‑1 (MQ=60)
               AAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATTGTTTCGCTGTTTGCCGGCGCCGTGCTGAATCAGACGGGGCAAGCCATCGAGAC                                                                                <  SRR4302544.158618/94‑1 (MQ=60)
               AAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGAC                                                                                <  SRR4302544.2292437/94‑1 (MQ=60)
                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGAT                                                                             <  SRR4302544.879364/94‑1 (MQ=60)
                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGAT                                                                             >  SRR4302544.3335042/1‑94 (MQ=60)
                   CTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATA                                                                            >  SRR4302544.474296/1‑94 (MQ=60)
                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAG                                                                           >  SRR4302544.2000971/1‑94 (MQ=60)
                     CCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGC                                                                          <  SRR4302544.557874/94‑1 (MQ=60)
                     CCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGC                                                                          >  SRR4302544.2542762/1‑94 (MQ=60)
                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCC                                                                         >  SRR4302544.4677483/1‑94 (MQ=60)
                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCC                                                                         >  SRR4302544.3496087/1‑94 (MQ=60)
                       TCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCA                                                                        >  SRR4302544.3174994/1‑94 (MQ=60)
                       TCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGACA                                                                        >  SRR4302544.986460/1‑94 (MQ=60)
                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGA                                                                     >  SRR4302544.3984430/1‑94 (MQ=60)
                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGA                                                                     >  SRR4302544.60727/1‑94 (MQ=60)
                             GCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCA                                                                  >  SRR4302544.3316377/1‑94 (MQ=60)
                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCAT                                                                 >  SRR4302544.3514148/1‑94 (MQ=60)
                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCAT                                                                 <  SRR4302544.4672431/94‑1 (MQ=60)
                               CGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATG                                                                >  SRR4302544.3888897/1‑94 (MQ=60)
                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                               <  SRR4302544.3657056/94‑1 (MQ=60)
                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                               <  SRR4302544.4499261/94‑1 (MQ=60)
                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                               >  SRR4302544.3433572/1‑94 (MQ=60)
                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGT                                                               >  SRR4302544.3047075/1‑94 (MQ=60)
                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCCTGTC                                                              >  SRR4302544.1205878/1‑94 (MQ=60)
                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTC                                                              >  SRR4302544.18835/1‑94 (MQ=60)
                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                           >  SRR4302544.177211/1‑94 (MQ=60)
                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                           >  SRR4302544.1074516/1‑94 (MQ=60)
                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGACAGGCATGACCATGTCGGC                                                           >  SRR4302544.3300602/1‑94 (MQ=60)
                                     TATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTC                                                          >  SRR4302544.2570845/1‑94 (MQ=60)
                                      ATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                         >  SRR4302544.4726187/1‑94 (MQ=60)
                                       TTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTA                                                        <  SRR4302544.2862180/94‑1 (MQ=60)
                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                    <  SRR4302544.3217723/94‑1 (MQ=60)
                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                    >  SRR4302544.540083/1‑94 (MQ=60)
                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                    <  SRR4302544.2391582/94‑1 (MQ=60)
                                            TATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                   >  SRR4302544.2003385/1‑94 (MQ=60)
                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTA                                                >  SRR4302544.2560752/1‑94 (MQ=60)
                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTA                                                <  SRR4302544.4608357/94‑1 (MQ=60)
                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                            <  SRR4302544.2924406/94‑1 (MQ=60)
                                                     ATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCC                                          >  SRR4302544.3121116/1‑94 (MQ=60)
                                                       GGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTG                                        >  SRR4302544.2844309/1‑94 (MQ=60)
                                                        GTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGA                                       >  SRR4302544.1926930/1‑94 (MQ=60)
                                                        GTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGA                                       <  SRR4302544.1252154/94‑1 (MQ=60)
                                                        GTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGA                                       >  SRR4302544.4091573/1‑94 (MQ=60)
                                                         TTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAC                                      <  SRR4302544.318946/94‑1 (MQ=60)
                                                           TCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTA                                    <  SRR4302544.2491099/94‑1 (MQ=60)
                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                   <  SRR4302544.4607405/94‑1 (MQ=60)
                                                             GCTGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATC                                  >  SRR4302544.1699666/1‑94 (MQ=60)
                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                <  SRR4302544.797917/94‑1 (MQ=60)
                                                                GTTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCG                               <  SRR4302544.4564930/94‑1 (MQ=60)
                                                                 TTTGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGC                              >  SRR4302544.2870869/1‑94 (MQ=60)
                                                                   TGCCGGCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                            <  SRR4302544.275727/94‑1 (MQ=60)
                                                                        GCACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                       >  SRR4302544.1674197/1‑94 (MQ=60)
                                                                         CACCGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATG                      <  SRR4302544.3416049/94‑1 (MQ=60)
                                                                            CGTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                   <  SRR4302544.3175746/94‑1 (MQ=60)
                                                                             GTGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                  >  SRR4302544.235227/1‑94 (MQ=60)
                                                                              TGCTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT                 <  SRR4302544.404699/94‑1 (MQ=60)
                                                                                CTGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT               <  SRR4302544.4010484/94‑1 (MQ=60)
                                                                                 TGAATCAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTA              <  SRR4302544.393097/94‑1 (MQ=60)
                                                                                    atcggacgGGGCAGGCCATCGAGCCGATCGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAA           <  SRR4302544.4256163/86‑1 (MQ=60)
                                                                                      CAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC         <  SRR4302544.3023648/94‑1 (MQ=60)
                                                                                      CAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC         >  SRR4302544.3169952/1‑94 (MQ=60)
                                                                                      CAGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC         <  SRR4302544.3023649/94‑1 (MQ=60)
                                                                                       AGACGGGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCG        <  SRR4302544.532492/94‑1 (MQ=60)
                                                                                          G‑GGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC    >  SRR4302544.684002/1‑94 (MQ=60)
                                                                                             GGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT  <  SRR4302544.3128515/94‑1 (MQ=60)
                                                                                             GGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT  <  SRR4302544.4468601/94‑1 (MQ=60)
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ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT  >  NC_000913/3418747‑3418932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑