Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,427,075 T→C V68A (GTC→GCC)  b3279 → putative transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,427,0750TC96.7% 718.6 / 8.6 243V68A (GTC→GCC) b3279putative transferase
Reads supporting (aligned to +/- strand):  ref base T (3/5);  new base C (104/130);  total (108/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 244 total aligned reads displayed.

GATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGC  >  NC_000913/3426986‑3427166
                                                                                         |                                                                                           
 aTCTGGCGGCTCGTTGTGATTCGGGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACt                                                                                         >  2:139684/1‑93 (MQ=255)
 aTCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGTAGTATGTTGCATGCCACt                                                                                         >  1:3392647/1‑93 (MQ=255)
 aTCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACt                                                                                         >  2:3632013/1‑93 (MQ=255)
    tGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCAt                                                                                      <  2:4285198/93‑1 (MQ=255)
     ggCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATa                                                                                     >  2:2306414/1‑93 (MQ=255)
         gCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATc                                                                                 <  2:1483450/93‑1 (MQ=255)
         gCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATc                                                                                 <  1:466091/93‑1 (MQ=255)
          cTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATcc                                                                                <  2:2624016/93‑1 (MQ=255)
           tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCt                                                                               >  1:4503830/1‑93 (MQ=255)
           tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCt                                                                               <  1:3002450/93‑1 (MQ=255)
           tCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCt                                                                               >  1:1739992/1‑93 (MQ=255)
            cGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTc                                                                              <  1:1629569/93‑1 (MQ=255)
            cGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTc                                                                              <  2:3791524/93‑1 (MQ=255)
                gtgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAc                                                                          >  2:1423665/1‑93 (MQ=255)
                gtgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAc                                                                          <  2:403284/93‑1 (MQ=255)
                gtgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAc                                                                          >  1:2491749/1‑93 (MQ=255)
                 tgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACa                                                                         >  2:3719798/1‑93 (MQ=255)
                 tgATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACa                                                                         <  1:510084/93‑1 (MQ=255)
                  gATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACaa                                                                        >  1:2851270/1‑93 (MQ=255)
                   aTTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAc                                                                       >  1:2715896/1‑93 (MQ=255)
                    ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                      >  1:1018241/1‑93 (MQ=255)
                    ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                      >  1:1847671/1‑93 (MQ=255)
                    ttCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAcc                                                                      <  2:2825938/93‑1 (MQ=255)
                       gTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAg                                                                   >  1:1596846/1‑93 (MQ=255)
                       gTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAg                                                                   <  2:3198561/93‑1 (MQ=255)
                        tGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGa                                                                  >  2:2970427/1‑93 (MQ=255)
                        tGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGa                                                                  <  2:4495020/93‑1 (MQ=255)
                         ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                                 >  1:3041493/1‑93 (MQ=255)
                         ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                                 >  1:1938431/1‑93 (MQ=255)
                         ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                                 <  1:96578/93‑1 (MQ=255)
                         ggAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTCGCATGCCACTCATAAATCCTCGTACAACCCAGAt                                                                 <  2:2976671/93‑1 (MQ=255)
                          gagaTGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATg                                                                >  2:4353647/1‑93 (MQ=255)
                          gagaTGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATg                                                                >  1:3191130/1‑93 (MQ=255)
                             aTGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCa                                                             >  1:1196679/1‑93 (MQ=255)
                              tGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCaa                                                            >  1:4140238/1‑93 (MQ=255)
                              tGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCaa                                                            >  1:4352850/1‑93 (MQ=255)
                               gTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAc                                                           <  2:726573/93‑1 (MQ=255)
                                tACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAcc                                                          <  2:2175740/93‑1 (MQ=255)
                                 aCATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAccc                                                         <  2:4044036/93‑1 (MQ=255)
                                  cATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCa                                                        <  2:576274/93‑1 (MQ=255)
                                   aTTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAt                                                       >  1:3163732/1‑93 (MQ=255)
                                    ttATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                                      <  1:736457/93‑1 (MQ=255)
                                    ttATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAtt                                                      <  1:1803403/93‑1 (MQ=255)
                                      aTGTACAGATCGGAGCGCGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                                                    <  1:43039/93‑1 (MQ=255)
                                      aTGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTaa                                                    >  2:3262650/1‑93 (MQ=255)
                                       tGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                                   >  1:2550017/1‑93 (MQ=255)
                                       tGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAc                                                   <  1:2251868/93‑1 (MQ=255)
                                       tGTACAGATCGGAGCACGCACCAAAATCCCGGATGGCAGGATGTTGCATGCCACTCATAAATCCTCGTACAAACCAGATGGCAACCCATTaaa                                                   >  2:1664724/1‑92 (MQ=255)
                                        gTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                                  <  2:1947254/93‑1 (MQ=255)
                                        gTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAcc                                                  <  1:2382109/93‑1 (MQ=255)
                                         tACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                                                 <  2:235804/93‑1 (MQ=255)
                                         tACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                                                 <  1:673135/93‑1 (MQ=255)
                                         tACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                                                 >  2:126176/1‑93 (MQ=255)
                                         tACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCa                                                 <  2:386309/93‑1 (MQ=255)
                                           cAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCACCCCATTAACCAtt                                               <  1:2002802/93‑1 (MQ=255)
                                            aGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTg                                              <  1:933901/93‑1 (MQ=255)
                                              aTCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGc                                            >  1:879140/1‑93 (MQ=255)
                                                 ggAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGaa                                         <  2:5399/93‑1 (MQ=255)
                                                 ggAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGaa                                         <  1:595999/93‑1 (MQ=255)
                                                  gAGGACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAg                                        >  2:3961485/1‑93 (MQ=255)
                                                   aggacggacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAGCCCATTAACCATTGGCGAAGa                                       <  2:4070495/90‑1 (MQ=255)
                                                    gcacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAt                                      >  1:2671324/1‑93 (MQ=255)
                                                     cacgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATg                                     >  2:3502552/1‑93 (MQ=255)
                                                       cgcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTc                                   <  1:2043983/93‑1 (MQ=255)
                                                        gcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                                  >  2:795544/1‑93 (MQ=255)
                                                        gcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                                  <  2:1570200/93‑1 (MQ=255)
                                                        gcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCa                                  >  1:2939808/1‑93 (MQ=255)
                                                        gcacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAAGGCAACCCATTAACCATTGGCGAAGATGTCa                                  <  1:3300463/93‑1 (MQ=255)
                                                         cacCAATATCCAGGATGGCTGTATGTTGCATGCCACTCATACATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                                 <  1:4205057/93‑1 (MQ=255)
                                                         cacCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAc                                 >  1:2327312/1‑93 (MQ=255)
                                                          acCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACt                                <  1:2061235/93‑1 (MQ=255)
                                                           ccAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTg                               >  1:1515376/1‑93 (MQ=255)
                                                            cAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGt                              >  2:1129708/1‑93 (MQ=255)
                                                              atatCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTg                            >  1:3580983/1‑93 (MQ=255)
                                                              atatCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTg                            <  2:221701/93‑1 (MQ=255)
                                                                atCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGt                          <  1:281558/93‑1 (MQ=255)
                                                                  ccAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTca                        <  1:46885/93‑1 (MQ=255)
                                                                   cAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac                       <  1:2086891/93‑1 (MQ=255)
                                                                   cAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcac                       <  2:3647125/93‑1 (MQ=255)
                                                                   cAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTAGCGAAGATGTCACTGTTGGTcac                       >  1:358729/1‑93 (MQ=255)
                                                                    aGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTcaca                      >  2:4215590/1‑93 (MQ=255)
                                                                     ggATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACaa                     >  1:765506/1‑93 (MQ=255)
                                                                     ggATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACaa                     <  1:4635922/93‑1 (MQ=255)
                                                                     ggATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACaa                     <  2:2141824/93‑1 (MQ=255)
                                                                      gATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAg                    >  1:3799531/1‑93 (MQ=255)
                                                                       aTGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAgg                   <  2:1303126/93‑1 (MQ=255)
                                                                        tGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAggg                  >  2:3855347/1‑92 (MQ=255)
                                                                        tGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGt                  <  1:2278073/93‑1 (MQ=255)
                                                                           cAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAt               >  2:4105214/1‑93 (MQ=255)
                                                                           cAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGAt               <  2:4663710/93‑1 (MQ=255)
                                                                               aTGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTc           >  2:3856559/1‑93 (MQ=255)
                                                                               aTGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTc           >  2:416602/1‑93 (MQ=255)
                                                                                tGTTTCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTcc          <  1:3919264/93‑1 (MQ=255)
                                                                                tGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTcc          <  1:4077675/93‑1 (MQ=255)
                                                                                 gTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCa         <  1:644507/93‑1 (MQ=255)
                                                                                    gCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACgg      <  2:1781799/93‑1 (MQ=255)
                                                                                     cATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGc     <  1:4425668/93‑1 (MQ=255)
                                                                                      aTGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCt    <  2:230701/93‑1 (MQ=255)
                                                                                      aTGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCt    <  2:2889098/93‑1 (MQ=255)
                                                                                        gTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGc  <  2:2011609/93‑1 (MQ=255)
                                                                                         |                                                                                           
GATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGC  >  NC_000913/3426986‑3427166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGC  >  NC_000913/3426982‑3427166
                                                                                             |                                                                                           
TGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGC                                                                                             <  SRR4302544.661796/94‑1 (MQ=60)
 GGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCC                                                                                            <  SRR4302544.2994861/94‑1 (MQ=60)
  GGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCA                                                                                           <  SRR4302544.1154795/94‑1 (MQ=60)
    GATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGTAGTATGTTGCATGCCACT                                                                                         >  SRR4302544.3425080/1‑94 (MQ=60)
    GATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACT                                                                                         <  SRR4302544.3621045/94‑1 (MQ=60)
    GATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACT                                                                                         <  SRR4302544.4711922/94‑1 (MQ=60)
       CTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCAT                                                                                      >  SRR4302544.1957476/1‑94 (MQ=60)
       CTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCAT                                                                                      <  SRR4302544.92678/94‑1 (MQ=60)
         GGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAA                                                                                    <  SRR4302544.2025678/94‑1 (MQ=60)
         GGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAA                                                                                    <  SRR4302544.2763150/94‑1 (MQ=60)
           CCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAAC                                                                                  >  SRR4302544.1072346/1‑94 (MQ=60)
            CGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATC                                                                                 >  SRR4302544.3069218/1‑94 (MQ=60)
            CGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATC                                                                                 >  SRR4302544.1475833/1‑94 (MQ=60)
             GCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCC                                                                                <  SRR4302544.470662/94‑1 (MQ=60)
             GCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCC                                                                                >  SRR4302544.2811019/1‑94 (MQ=60)
              CTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCT                                                                               >  SRR4302544.1757030/1‑94 (MQ=60)
              CTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCT                                                                               >  SRR4302544.4549003/1‑94 (MQ=60)
               TCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTC                                                                              <  SRR4302544.3031231/94‑1 (MQ=60)
                CGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCG                                                                             <  SRR4302544.1645612/94‑1 (MQ=60)
                CGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCAAGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCG                                                                             >  SRR4302544.1643376/1‑94 (MQ=60)
                 GTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGT                                                                            <  SRR4302544.295544/94‑1 (MQ=60)
                   TGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAC                                                                          >  SRR4302544.2624896/1‑94 (MQ=60)
                   TGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAC                                                                          >  SRR4302544.2515610/1‑94 (MQ=60)
                   TGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAC                                                                          <  SRR4302544.1691583/94‑1 (MQ=60)
                   TGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTAC                                                                          <  SRR4302544.626029/94‑1 (MQ=60)
                    GTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACA                                                                         <  SRR4302544.2660471/94‑1 (MQ=60)
                     TGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAA                                                                        <  SRR4302544.515119/94‑1 (MQ=60)
                     TGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAA                                                                        >  SRR4302544.2878812/1‑94 (MQ=60)
                      GATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAAC                                                                       >  SRR4302544.2742036/1‑94 (MQ=60)
                       ATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATCAATCCTCGTACAACC                                                                      >  SRR4302544.1647468/1‑94 (MQ=60)
                       ATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACC                                                                      >  SRR4302544.1865559/1‑94 (MQ=60)
                       ATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACC                                                                      >  SRR4302544.1028406/1‑94 (MQ=60)
                        TTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCC                                                                     <  SRR4302544.4793760/94‑1 (MQ=60)
                        TTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCC                                                                     >  SRR4302544.208238/1‑94 (MQ=60)
                         TCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCA                                                                    <  SRR4302544.2538083/94‑1 (MQ=60)
                          CGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAG                                                                   >  SRR4302544.1612577/1‑94 (MQ=60)
                            TGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAT                                                                 >  SRR4302544.1957195/1‑94 (MQ=60)
                            TGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAT                                                                 >  SRR4302544.3070597/1‑94 (MQ=60)
                             GGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATG                                                                <  SRR4302544.97462/94‑1 (MQ=60)
                             GGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATG                                                                >  SRR4302544.3221534/1‑94 (MQ=60)
                             GGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATG                                                                <  SRR4302544.3478758/94‑1 (MQ=60)
                             GGAGATGTACATTATGTACAGATCGGAGCACGCACCAATAGCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATG                                                                <  SRR4302544.2395629/94‑1 (MQ=60)
                                GATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCA                                                             >  SRR4302544.1208544/1‑94 (MQ=60)
                                GATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCA                                                             >  SRR4302544.4128064/1‑94 (MQ=60)
                                 ATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAA                                                            >  SRR4302544.3303988/1‑94 (MQ=60)
                                 ATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAA                                                            >  SRR4302544.4181227/1‑94 (MQ=60)
                                 ATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAA                                                            >  SRR4302544.4397063/1‑94 (MQ=60)
                                  TGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAC                                                           >  SRR4302544.540311/1‑94 (MQ=60)
                                   GTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACC                                                          >  SRR4302544.783284/1‑94 (MQ=60)
                                     ACATTATGTACAGATCGGAGCACGCACCAGTATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCA                                                        <  SRR4302544.1952856/94‑1 (MQ=60)
                                      CATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCAT                                                       >  SRR4302544.3193823/1‑94 (MQ=60)
                                       ATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT                                                      >  SRR4302544.947882/1‑94 (MQ=60)
                                       ATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATT                                                      <  SRR4302544.2142875/94‑1 (MQ=60)
                                        TTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                                     <  SRR4302544.743660/94‑1 (MQ=60)
                                        TTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                                     <  SRR4302544.1821022/94‑1 (MQ=60)
                                        TTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                                     <  SRR4302544.628715/94‑1 (MQ=60)
                                        TTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTA                                                     <  SRR4302544.652076/94‑1 (MQ=60)
                                          ATGTACAGATCGGAGCGCGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                                   <  SRR4302544.43462/94‑1 (MQ=60)
                                          ATGTACAGATCGGAGCGCGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                                   <  SRR4302544.43461/94‑1 (MQ=60)
                                          ATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAAC                                                   >  SRR4302544.2574502/1‑94 (MQ=60)
                                           TGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACC                                                  <  SRR4302544.2273032/94‑1 (MQ=60)
                                            GTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCA                                                 <  SRR4302544.2404663/94‑1 (MQ=60)
                                             TACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCAT                                                <  SRR4302544.679787/94‑1 (MQ=60)
                                              ACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATT                                               <  SRR4302544.4676580/94‑1 (MQ=60)
                                              ACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAAACCATTAACCATT                                               >  SRR4302544.3143693/1‑94 (MQ=60)
                                               CAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCACCCCATTAACCATTG                                              <  SRR4302544.2022255/94‑1 (MQ=60)
                                               CAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                              <  SRR4302544.75526/94‑1 (MQ=60)
                                               CAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                              <  SRR4302544.4424244/94‑1 (MQ=60)
                                               CAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTG                                              <  SRR4302544.2068915/94‑1 (MQ=60)
                                                AGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGG                                             <  SRR4302544.943102/94‑1 (MQ=60)
                                                 GATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGC                                            >  SRR4302544.887857/1‑94 (MQ=60)
                                                  ATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCG                                           <  SRR4302544.229938/94‑1 (MQ=60)
                                                   TCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGA                                          >  SRR4302544.1593878/1‑94 (MQ=60)
                                                   TCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGA                                          >  SRR4302544.695451/1‑94 (MQ=60)
                                                     gtagcgcgcacccatatcgaggagtGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                                        <  SRR4302544.3496998/69‑1 (MQ=60)
                                                     ctacgagcCACCAATATCCAGGGTGGCAGTATGCTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCACCCCATTAACCATTGGCGAAG                                        <  SRR4302544.502535/86‑1 (MQ=60)
                                                     GGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                                        <  SRR4302544.502536/94‑1 (MQ=60)
                                                     GGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAG                                        <  SRR4302544.601845/94‑1 (MQ=60)
                                                      GAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGA                                       >  SRR4302544.3308073/1‑94 (MQ=60)
                                                       AGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                                      <  SRR4302544.2379535/94‑1 (MQ=60)
                                                       AGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                                      <  SRR4302544.4218593/94‑1 (MQ=60)
                                                       AGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                                      >  SRR4302544.2696931/1‑94 (MQ=60)
                                                       AGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGAT                                      >  SRR4302544.3360404/1‑94 (MQ=60)
                                                        GCACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATG                                     <  SRR4302544.4445433/94‑1 (MQ=60)
                                                         CACGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGT                                    >  SRR4302544.1821232/1‑94 (MQ=60)
                                                           CGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                                  >  SRR4302544.369243/1‑94 (MQ=60)
                                                           CGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                                  >  SRR4302544.2968041/1‑94 (MQ=60)
                                                           CGCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCA                                  <  SRR4302544.2063692/94‑1 (MQ=60)
                                                            GCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAC                                 <  SRR4302544.3029124/94‑1 (MQ=60)
                                                            GCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCAC                                 >  SRR4302544.2349375/1‑94 (MQ=60)
                                                            GCACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGAAGGCAACCCATTAACCATTGGCGAAGATGTCAC                                 <  SRR4302544.3331899/94‑1 (MQ=60)
                                                             CACCAATATCCAGGATGGCTGTATGTTGCATGCCACTCATACATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                                <  SRR4302544.4247206/94‑1 (MQ=60)
                                                             CACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                                <  SRR4302544.321743/94‑1 (MQ=60)
                                                             CACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                                <  SRR4302544.4372403/94‑1 (MQ=60)
                                                             CACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACT                                >  SRR4302544.2703348/1‑94 (MQ=60)
                                                              ACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTG                               >  SRR4302544.1530409/1‑94 (MQ=60)
                                                              ACCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTG                               <  SRR4302544.2081058/94‑1 (MQ=60)
                                                               CCAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGT                              >  SRR4302544.2499281/1‑94 (MQ=60)
                                                                CAATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTT                             <  SRR4302544.4546651/94‑1 (MQ=60)
                                                                 AATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG                            <  SRR4302544.4695866/94‑1 (MQ=60)
                                                                 AATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTG                            >  SRR4302544.3615336/1‑94 (MQ=60)
                                                                  ATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGACGATGTCACTGTTGG                           >  SRR4302544.3866410/1‑94 (MQ=60)
                                                                  ATATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGG                           <  SRR4302544.790060/94‑1 (MQ=60)
                                                                    ATCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTC                         <  SRR4302544.284492/94‑1 (MQ=60)
                                                                     TCCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCA                        >  SRR4302544.2333104/1‑94 (MQ=60)
                                                                      CCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC                       >  SRR4302544.1571936/1‑94 (MQ=60)
                                                                      CCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCAC                       <  SRR4302544.47352/94‑1 (MQ=60)
                                                                      CCAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTAGCGAAGATGTCACTGTTGGTCAC                       >  SRR4302544.362403/1‑94 (MQ=60)
                                                                       CAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACA                      >  SRR4302544.3781412/1‑94 (MQ=60)
                                                                       CAGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACA                      <  SRR4302544.2106969/94‑1 (MQ=60)
                                                                        AGGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAA                     >  SRR4302544.772964/1‑94 (MQ=60)
                                                                         GGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG                    <  SRR4302544.2046958/94‑1 (MQ=60)
                                                                         GGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG                    >  SRR4302544.3837162/1‑94 (MQ=60)
                                                                         GGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG                    <  SRR4302544.4681857/94‑1 (MQ=60)
                                                                         GGATGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAG                    <  SRR4302544.4597075/94‑1 (MQ=60)
                                                                            TGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG                 <  SRR4302544.2299525/94‑1 (MQ=60)
                                                                            TGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG                 <  SRR4302544.1857468/94‑1 (MQ=60)
                                                                            TGGCAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTG                 <  SRR4302544.3034418/94‑1 (MQ=60)
                                                                               CAGTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATG              >  SRR4302544.228403/1‑94 (MQ=60)
                                                                                 GTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCT            >  SRR4302544.4141654/1‑94 (MQ=60)
                                                                                 GTATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCT            <  SRR4302544.713524/94‑1 (MQ=60)
                                                                                   ATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCC          >  SRR4302544.4323570/1‑94 (MQ=60)
                                                                                   ATGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCC          >  SRR4302544.2090088/1‑94 (MQ=60)
                                                                                    TGTTTCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCA         <  SRR4302544.3958189/94‑1 (MQ=60)
                                                                                    TGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCA         <  SRR4302544.4118253/94‑1 (MQ=60)
                                                                                    TGTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCA         <  SRR4302544.2949033/94‑1 (MQ=60)
                                                                                     GTTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCAC        <  SRR4302544.650874/94‑1 (MQ=60)
                                                                                      TTGCATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACG       <  SRR4302544.2871188/94‑1 (MQ=60)
                                                                                       TGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGG      >  SRR4302544.4030236/1‑94 (MQ=60)
                                                                                         CATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCT    <  SRR4302544.4470320/94‑1 (MQ=60)
                                                                                          ATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTG   >  SRR4302544.4406295/1‑94 (MQ=60)
                                                                                          ATGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTG   <  SRR4302544.1764220/94‑1 (MQ=60)
                                                                                           TGCCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGC  <  SRR4302544.2766842/94‑1 (MQ=60)
                                                                                             |                                                                                           
TGGGGATCTGGCCGCTCGTTGTGATTCGTGGAGATGTACATTATGTACAGATCGGAGCACGCACCAATATCCAGGATGGCAGTATGTTGCATGTCACTCATAAATCCTCGTACAACCCAGATGGCAACCCATTAACCATTGGCGAAGATGTCACTGTTGGTCACAAGGTGATGCTCCACGGCTGC  >  NC_000913/3426982‑3427166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑