Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,433,176	T→C	R111R (CGT→CGC)	sun →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,433,176	0	T	C	95.7%	619.3 / 7.8	209	R111R (CGT→CGC)	sun	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (5/4); new base C (72/128); total (77/132)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

TATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGC  >  NC_000913/3433086‑3433265

tATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGccc                                                                                         <  1:1621477/93‑1 (MQ=255)

 aTGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCg                                                                                        <  1:2902053/93‑1 (MQ=255)

   ggttggttTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCa                                                                                      >  2:4355244/1‑93 (MQ=255)

       ggttTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACtt                                                                                  <  1:3879361/93‑1 (MQ=255)

          ttGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTaaa                                                                               <  2:4293406/93‑1 (MQ=255)

           tGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAg                                                                              >  2:1972059/1‑93 (MQ=255)

            gTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAgg                                                                             <  2:1924308/93‑1 (MQ=255)

            gTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAgg                                                                             <  2:2809386/93‑1 (MQ=255)

            gTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAgg                                                                             <  2:3265303/93‑1 (MQ=255)

            gTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGGTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAgg                                                                             <  1:744295/93‑1 (MQ=255)

               tCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGtt                                                                          <  2:4640602/93‑1 (MQ=255)

               tCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGtt                                                                          <  2:1341420/93‑1 (MQ=255)

                cAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTg                                                                         <  1:2320696/93‑1 (MQ=255)

                 accTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGa                                                                        <  2:1665380/91‑1 (MQ=255)

                 aaCTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGa                                                                        >  2:3931119/1‑93 (MQ=255)

                  aCTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGAt                                                                       <  2:2618682/93‑1 (MQ=255)

                  aCTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGAt                                                                       >  1:1383387/1‑93 (MQ=255)

                  aCTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGAt                                                                       >  2:2132775/1‑93 (MQ=255)

                    tGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATaa                                                                     <  1:4310009/93‑1 (MQ=255)

                     gCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATaaa                                                                    <  2:330295/93‑1 (MQ=255)

                      cTTTATACCCGCGTTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAAc                                                                   <  1:1091712/93‑1 (MQ=255)

                      cTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAAc                                                                   >  1:4446557/1‑93 (MQ=255)

                        ttATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACgg                                                                 <  1:4518589/93‑1 (MQ=255)

                        ttATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACgg                                                                 <  1:3528785/93‑1 (MQ=255)

                         tataCCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGgt                                                                <  1:4487566/93‑1 (MQ=255)

                          ataCCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGgtg                                                               >  1:3665939/1‑93 (MQ=255)

                          ataCCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGgtg                                                               >  2:2369386/1‑93 (MQ=255)

                           taCCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGgtgt                                                              <  1:2131198/93‑1 (MQ=255)

                             cccGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTAt                                                            <  1:1944529/93‑1 (MQ=255)

                             cccGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTAt                                                            >  1:89036/1‑93 (MQ=255)

                               cGCATTCCGCCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTa                                                          <  2:659728/93‑1 (MQ=255)

                                gCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTAc                                                         <  2:3849141/93‑1 (MQ=255)

                                gCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTAc                                                         <  2:4141353/93‑1 (MQ=255)

                                gCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTAc                                                         >  1:4055327/1‑93 (MQ=255)

                                   ttCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGcc                                                      <  1:2279903/93‑1 (MQ=255)

                                   ttCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGcc                                                      <  2:4249531/93‑1 (MQ=255)

                                      cACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGt                                                   >  1:4149928/1‑93 (MQ=255)

                                      cACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGt                                                   <  2:629842/93‑1 (MQ=255)

                                      cACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGt                                                   <  2:3013853/93‑1 (MQ=255)

                                       aCCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGtt                                                  <  2:3577194/93‑1 (MQ=255)

                                       aCCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGtt                                                  >  2:1656250/1‑93 (MQ=255)

                                         cTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTcc                                                <  1:3570527/93‑1 (MQ=255)

                                         cTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTcc                                                >  2:3100391/1‑93 (MQ=255)

                                          tCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACTGTGTATTACGCCAGTTCCa                                               <  2:3547957/93‑1 (MQ=255)

                                          tCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGCTCCa                                               >  2:4242734/1‑93 (MQ=255)

                                           cATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAg                                              <  1:269574/93‑1 (MQ=255)

                                           cATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAg                                              <  1:2369386/93‑1 (MQ=255)

                                            aTGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGc                                             >  1:3015136/1‑93 (MQ=255)

                                               ctgcGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTc                                          <  1:1332211/93‑1 (MQ=255)

                                               ctgcGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTc                                          >  2:2287638/1‑93 (MQ=255)

                                                tgcGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCa                                         <  2:4310112/93‑1 (MQ=255)

                                                tgcGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCa                                         <  1:1230972/93‑1 (MQ=255)

                                                tgcGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCa                                         >  1:3555521/1‑93 (MQ=255)

                                                    ctggctgAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCaa                                     >  1:3540831/1‑93 (MQ=255)

                                                      ggctgAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCaaga                                   <  1:1773235/93‑1 (MQ=255)

                                                      ggctgAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCaaga                                   >  2:3349014/1‑93 (MQ=255)

                                                       gctgAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCaagaa                                  <  1:2727104/93‑1 (MQ=255)

                                                        ctaaaaCGGGTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCaagaag                                 <  1:1884535/90‑1 (MQ=255)

                                                         tgAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAagaaga                                <  2:3137827/93‑1 (MQ=255)

                                                            aaCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGtt                             <  2:3284049/93‑1 (MQ=255)

                                                            aaCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAATTCCAGCGTCAGCAAGAAGAGta                             >  2:2591645/1‑92 (MQ=255)

                                                             aCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGtta                            >  2:2522705/1‑93 (MQ=255)

                                                             aCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGtta                            <  1:4191517/93‑1 (MQ=255)

                                                             aCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGtta                            >  2:1844441/1‑93 (MQ=255)

                                                              cGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttat                           <  2:3649805/93‑1 (MQ=255)

                                                               ggTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatt                          <  2:2027331/93‑1 (MQ=255)

                                                               ggTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatt                          <  2:419031/93‑1 (MQ=255)

                                                                gTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatta                         <  1:3733989/93‑1 (MQ=255)

                                                                gTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatta                         <  1:983782/93‑1 (MQ=255)

                                                                gTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatta                         >  2:171917/1‑93 (MQ=255)

                                                                gTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGttatta                         <  1:36357/93‑1 (MQ=255)

                                                                 ttGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAg                        >  1:1670847/1‑93 (MQ=255)

                                                                  tGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGc                       <  1:4557713/93‑1 (MQ=255)

                                                                      ggCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAg                   <  2:1524770/93‑1 (MQ=255)

                                                                      ggCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAg                   <  2:2909332/93‑1 (MQ=255)

                                                                      ggCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAg                   >  1:867640/1‑93 (MQ=255)

                                                                       gcgcTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGt                  >  1:2756294/1‑93 (MQ=255)

                                                                       gcgcTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGt                  <  1:3055422/93‑1 (MQ=255)

                                                                        cgcTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGtt                 <  1:4098175/93‑1 (MQ=255)

                                                                         gcTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGttt                <  2:4202327/93‑1 (MQ=255)

                                                                            aTCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAAt             <  1:2553787/93‑1 (MQ=255)

                                                                            aTCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAAt             >  2:4568177/1‑93 (MQ=255)

                                                                             tCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAAtc            >  2:2759898/1‑92 (MQ=255)

                                                                             tCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATg            <  1:1129959/93‑1 (MQ=255)

                                                                              cGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGc           >  1:2596316/1‑93 (MQ=255)

                                                                               gCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGcc          <  2:3690150/93‑1 (MQ=255)

                                                                               gCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGcc          >  2:34730/1‑93 (MQ=255)

                                                                               gCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGcc          <  1:4671060/93‑1 (MQ=255)

                                                                                cAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCa         <  1:3363447/93‑1 (MQ=255)

                                                                                 aaTTAAGCGCCCGTAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAg        >  1:3137106/1‑93 (MQ=255)

                                                                                 aaTTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAg        <  2:3224817/93‑1 (MQ=255)

                                                                                   ttAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTg      >  1:1253771/1‑93 (MQ=255)

                                                                                    tAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGa     <  2:3792829/93‑1 (MQ=255)

                                                                                    tAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGa     <  2:3429817/93‑1 (MQ=255)

                                                                                    tAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGa     <  1:149915/93‑1 (MQ=255)

                                                                                     aaGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGAt    <  2:4014814/93‑1 (MQ=255)

                                                                                     aaGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGAt    <  1:2214041/93‑1 (MQ=255)

                                                                                     aaGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGAt    <  2:677366/93‑1 (MQ=255)

                                                                                      aGCGCCCGCAACTTAAAGGGTTGATAAGCGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATg   <  1:1790114/93‑1 (MQ=255)

                                                                                       gcgcCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGc  >  2:2636493/1‑93 (MQ=255)

TATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGC  >  NC_000913/3433086‑3433265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGCACGT  >  NC_000913/3433084‑3433269

ATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCC                                                                                              <  SRR4302544.813594/94‑1 (MQ=60)

ATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCC                                                                                              >  SRR4302544.4448671/1‑94 (MQ=60)

  TATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCG                                                                                            <  SRR4302544.1637451/94‑1 (MQ=60)

   ATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGC                                                                                           <  SRR4302544.2929997/94‑1 (MQ=60)

    TGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCA                                                                                          >  SRR4302544.2814361/1‑94 (MQ=60)

       TTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACT                                                                                       <  SRR4302544.2788027/94‑1 (MQ=60)

        TGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTT                                                                                      >  SRR4302544.894843/1‑94 (MQ=60)

         GGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTA                                                                                     <  SRR4302544.3917880/94‑1 (MQ=60)

          GTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAA                                                                                    <  SRR4302544.3056537/94‑1 (MQ=60)

           TTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAA                                                                                   >  SRR4302544.4351218/1‑94 (MQ=60)

            TTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAG                                                                                  <  SRR4302544.1728900/94‑1 (MQ=60)

            TTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAG                                                                                  <  SRR4302544.422859/94‑1 (MQ=60)

            TTGTATCAAATGCGTTATACCCGCATGCCACCTCATGCTGCGCTGGCTGAAGCGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAGCTTAAAG                                                                                  <  SRR4302544.422858/94‑1 (MQ=60)

             TGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGG                                                                                 <  SRR4302544.4712689/94‑1 (MQ=60)

              GTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGG                                                                                <  SRR4302544.751585/94‑1 (MQ=60)

              GTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGG                                                                                <  SRR4302544.254809/94‑1 (MQ=60)

              GTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGGTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGG                                                                                <  SRR4302544.751584/94‑1 (MQ=60)

               TATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGT                                                                               >  SRR4302544.2207582/1‑94 (MQ=60)

               TATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGT                                                                               <  SRR4302544.2660679/94‑1 (MQ=60)

                 TCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTG                                                                             >  SRR4302544.2590834/1‑94 (MQ=60)

                  CAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGA                                                                            <  SRR4302544.2342683/94‑1 (MQ=60)

                   AACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGAT                                                                           >  SRR4302544.1397206/1‑94 (MQ=60)

                     CTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAA                                                                         <  SRR4302544.2356456/94‑1 (MQ=60)

                      TGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAA                                                                        <  SRR4302544.4353940/94‑1 (MQ=60)

                       GCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAAC                                                                       >  SRR4302544.4491364/1‑94 (MQ=60)

                        CTTTATACCCGCGTTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACG                                                                      <  SRR4302544.1102573/94‑1 (MQ=60)

                        CTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACG                                                                      <  SRR4302544.1182049/94‑1 (MQ=60)

                        CTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACG                                                                      <  SRR4302544.2766414/94‑1 (MQ=60)

                         TTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGG                                                                     >  SRR4302544.1904905/1‑94 (MQ=60)

                          TTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGT                                                                    <  SRR4302544.4563840/94‑1 (MQ=60)

                          TTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGT                                                                    <  SRR4302544.3562522/94‑1 (MQ=60)

                           TATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTG                                                                   >  SRR4302544.3702495/1‑94 (MQ=60)

                           TATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTG                                                                   <  SRR4302544.4532641/94‑1 (MQ=60)

                            ATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGT                                                                  >  SRR4302544.4517833/1‑94 (MQ=60)

                             TACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTA                                                                 <  SRR4302544.2151603/94‑1 (MQ=60)

                              ACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTAT                                                                >  SRR4302544.3572319/1‑94 (MQ=60)

                              ACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTAT                                                                <  SRR4302544.2289608/94‑1 (MQ=60)

                              ACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTAT                                                                >  SRR4302544.89877/1‑94 (MQ=60)

                               CCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATT                                                               <  SRR4302544.1963355/94‑1 (MQ=60)

                               CCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATT                                                               >  SRR4302544.1342757/1‑94 (MQ=60)

                                CCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTA                                                              >  SRR4302544.3122870/1‑94 (MQ=60)

                                CCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGAGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTA                                                              >  SRR4302544.358182/1‑94 (MQ=60)

                                CCGCATTCCACCTCATGCTGCACTGGCTGAAACGGTTGAAGGCGCTATCGCAATAAAGCCCGCGCAACTTAAAGGGTTGATAAACGGTGTATTA                                                              <  SRR4302544.2724519/94‑1 (MQ=60)

                                 CGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTAC                                                             >  SRR4302544.374714/1‑94 (MQ=60)

                                 CGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTAC                                                             >  SRR4302544.4095718/1‑94 (MQ=60)

                                  GCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACG                                                            <  SRR4302544.3667672/94‑1 (MQ=60)

                                     TTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCA                                                         <  SRR4302544.2301384/94‑1 (MQ=60)

                                      TCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAG                                                        <  SRR4302544.2377252/94‑1 (MQ=60)

                                       CCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGT                                                       <  SRR4302544.455712/94‑1 (MQ=60)

                                       CCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGT                                                       >  SRR4302544.4191019/1‑94 (MQ=60)

                                          CCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCC                                                    >  SRR4302544.3264954/1‑94 (MQ=60)

                                           CTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCA                                                   <  SRR4302544.3604764/94‑1 (MQ=60)

                                           CTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCA                                                   >  SRR4302544.3424057/1‑94 (MQ=60)

                                             CATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCACTTTAAAGGGTTGATAAACGGGGAATTACGCCAGTTCCAGG                                                 >  SRR4302544.2977233/1‑94 (MQ=60)

                                             CATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGC                                                 <  SRR4302544.2391807/94‑1 (MQ=60)

                                             CATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGC                                                 <  SRR4302544.272418/94‑1 (MQ=60)

                                             CATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGC                                                 >  SRR4302544.3044037/1‑94 (MQ=60)

                                             CATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGC                                                 <  SRR4302544.4163895/94‑1 (MQ=60)

                                              ATGCTGCGCTGGCTGAAAAGGTTGAAGGCGCTATCGCCATTAAGCGCCCGCCACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCG                                                >  SRR4302544.93972/1‑94 (MQ=60)

                                                 CTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCA                                             <  SRR4302544.4028621/94‑1 (MQ=60)

                                                 CTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCA                                             <  SRR4302544.1345325/94‑1 (MQ=60)

                                                 CTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCA                                             >  SRR4302544.3589596/1‑94 (MQ=60)

                                                  TGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAG                                            <  SRR4302544.1243147/94‑1 (MQ=60)

                                                  TGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAG                                            <  SRR4302544.4386821/94‑1 (MQ=60)

                                                     GCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAA                                         >  SRR4302544.3574721/1‑94 (MQ=60)

                                                        GGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAA                                      <  SRR4302544.1790591/94‑1 (MQ=60)

                                                         GCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAG                                     <  SRR4302544.2753380/94‑1 (MQ=60)

                                                          CTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGA                                    <  SRR4302544.1902755/94‑1 (MQ=60)

                                                          CTAAAACGGGTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGA                                    <  SRR4302544.1902754/94‑1 (MQ=60)

                                                           TGCAACGGTTGAGAGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGATCCAGCGTCAGCAAGAAAAG                                   <  SRR4302544.4254568/94‑1 (MQ=60)

                                                           TGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAG                                   <  SRR4302544.151385/94‑1 (MQ=60)

                                                           TGAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAG                                   >  SRR4302544.461260/1‑94 (MQ=60)

                                                            GAAACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGT                                  <  SRR4302544.2109246/94‑1 (MQ=60)

                                                              AACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTA                                >  SRR4302544.1863475/1‑94 (MQ=60)

                                                               ACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTAT                               <  SRR4302544.4116576/94‑1 (MQ=60)

                                                               ACGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTAT                               <  SRR4302544.4233030/94‑1 (MQ=60)

                                                                CGGTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATT                              <  SRR4302544.1661109/94‑1 (MQ=60)

                                                                  GTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAG                            <  SRR4302544.993424/94‑1 (MQ=60)

                                                                  GTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAG                            <  SRR4302544.3771110/94‑1 (MQ=60)

                                                                  GTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAG                            >  SRR4302544.3675472/1‑94 (MQ=60)

                                                                  GTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAG                            <  SRR4302544.36711/94‑1 (MQ=60)

                                                                  GTTGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAG                            >  SRR4302544.1687311/1‑94 (MQ=60)

                                                                    TGAAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCC                          <  SRR4302544.4603183/94‑1 (MQ=60)

                                                                      AAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGA                        >  SRR4302544.3765927/1‑94 (MQ=60)

                                                                      AAGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGA                        <  SRR4302544.488934/94‑1 (MQ=60)

                                                                       AGGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAG                       >  SRR4302544.876228/1‑94 (MQ=60)

                                                                        GGCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGT                      >  SRR4302544.2782824/1‑94 (MQ=60)

                                                                         GCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTT                     <  SRR4302544.3084595/94‑1 (MQ=60)

                                                                         GCGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTT                     <  SRR4302544.3475002/94‑1 (MQ=60)

                                                                          CGCTATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTT                    <  SRR4302544.4138957/94‑1 (MQ=60)

                                                                             TATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAAT                 >  SRR4302544.3861989/1‑94 (MQ=60)

                                                                              ATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATG                <  SRR4302544.2578304/94‑1 (MQ=60)

                                                                              ATCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATC                >  SRR4302544.1255461/1‑94 (MQ=60)

                                                                               TCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGC               <  SRR4302544.1141219/94‑1 (MQ=60)

                                                                               TCGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGC               >  SRR4302544.2621276/1‑94 (MQ=60)

                                                                                CGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCC              <  SRR4302544.4583306/94‑1 (MQ=60)

                                                                                CGCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCC              <  SRR4302544.179569/94‑1 (MQ=60)

                                                                                 GCAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCA             <  SRR4302544.4717228/94‑1 (MQ=60)

                                                                                  CAATTAAGCGCCCGTAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAG            >  SRR4302544.3166917/1‑94 (MQ=60)

                                                                                  CAATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAG            <  SRR4302544.3395549/94‑1 (MQ=60)

                                                                                   AATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGT           >  SRR4302544.3593875/1‑94 (MQ=60)

                                                                                   AATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGT           >  SRR4302544.1404274/1‑94 (MQ=60)

                                                                                   AATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGT           >  SRR4302544.1404275/1‑94 (MQ=60)

                                                                                    ATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTG          <  SRR4302544.2316275/94‑1 (MQ=60)

                                                                                    ATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTG          >  SRR4302544.1266106/1‑94 (MQ=60)

                                                                                    ATTAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTG          >  SRR4302544.1376397/1‑94 (MQ=60)

                                                                                      TAAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGAT        <  SRR4302544.151351/94‑1 (MQ=60)

                                                                                       AAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATG       <  SRR4302544.2234871/94‑1 (MQ=60)

                                                                                       AAGCGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATG       >  SRR4302544.1126936/1‑94 (MQ=60)

                                                                                        AGCGCCCGCAACTTAAAGGGTTGATAAGCGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGC      <  SRR4302544.1807613/94‑1 (MQ=60)

                                                                                          CGCCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGCAC    <  SRR4302544.2410692/94‑1 (MQ=60)

                                                                                            CCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGaccgt  >  SRR4302544.2581723/1‑89 (MQ=60)

                                                                                            CCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGCACGT  >  SRR4302544.3198924/1‑94 (MQ=60)

ATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTTAAAGGGTTGATAAACGGTGTATTACGCCAGTTCCAGCGTCAGCAAGAAGAGTTATTAGCCGAGTTTAATGCCAGTGATGCACGT  >  NC_000913/3433084‑3433269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`