Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,109 G→A P152L (CCG→CTG)  rplC ← 50S ribosomal subunit protein L3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1090GA98.5% 720.6 / ‑2.8 204P152L (CCG→CTG) rplC50S ribosomal subunit protein L3
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (106/95);  total (108/96)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 210 total aligned reads displayed.

GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198
                                                                                          |                                                                                         
gCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         <  2:2998103/93‑1 (MQ=255)
gCGTCAACGCGGACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGa                                                                                         >  2:2748894/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  1:264818/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       <  2:2407384/93‑1 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       >  2:3906658/1‑93 (MQ=255)
  gTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGt                                                                                       <  1:2450635/93‑1 (MQ=255)
   tCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTc                                                                                      <  2:4260835/93‑1 (MQ=255)
   tCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTc                                                                                      >  1:2375975/1‑93 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTCCACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     <  1:1661725/93‑1 (MQ=255)
    cAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCt                                                                                     >  1:1211016/1‑93 (MQ=255)
     aaCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTg                                                                                    <  1:406725/93‑1 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTTTTGCCTTTGAACACTTTGCCCACAGTCTgg                                                                                   >  1:3847565/1‑93 (MQ=255)
      aCGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTgg                                                                                   <  1:388498/93‑1 (MQ=255)
        gcgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGtt                                                                                 >  2:3833002/1‑93 (MQ=255)
         cgTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTc                                                                                >  1:1912652/1‑93 (MQ=255)
          gTACTACGTCAAGGCTCTGAACGGTTATACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCt                                                                               >  1:1557107/1‑93 (MQ=255)
          gTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCt                                                                               >  1:2755388/1‑93 (MQ=255)
           tactacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTg                                                                              >  1:489150/1‑93 (MQ=255)
            actacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGa                                                                             <  1:4374004/93‑1 (MQ=255)
              tacGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAcc                                                                           <  1:1858792/93‑1 (MQ=255)
               acGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCg                                                                          >  1:21363/1‑93 (MQ=255)
               acGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCg                                                                          >  2:289541/1‑93 (MQ=255)
               acGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCg                                                                          >  2:1009783/1‑93 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGCCCGa                                                                         >  2:1054956/1‑93 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGa                                                                         <  1:2308641/93‑1 (MQ=255)
                cGTCAAGGCTCTGAACGGTTACACGTGCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGa                                                                         <  2:749623/93‑1 (MQ=255)
                   cAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAg                                                                      <  1:2493382/93‑1 (MQ=255)
                       gCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAcc                                                                  <  2:1919413/93‑1 (MQ=255)
                       gCTCCGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAcc                                                                  <  1:2744515/93‑1 (MQ=255)
                        ctctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAAccc                                                                 <  2:2239485/93‑1 (MQ=255)
                         tctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCg                                                                <  1:813806/93‑1 (MQ=255)
                         tctGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCg                                                                <  1:1383187/93‑1 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGa                                                              >  1:2565299/1‑93 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGa                                                              >  1:466349/1‑93 (MQ=255)
                           tGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGa                                                              <  1:3548555/93‑1 (MQ=255)
                            gAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGaa                                                             >  1:3755273/1‑93 (MQ=255)
                             aaCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  1:3792556/93‑1 (MQ=255)
                             aaCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            >  2:4566105/1‑93 (MQ=255)
                             aaCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAc                                                            <  2:3137390/93‑1 (MQ=255)
                              aCGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcg                                                           >  2:665588/1‑93 (MQ=255)
                               cgtttACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  2:2584842/90‑1 (MQ=255)
                               cGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  1:4011307/93‑1 (MQ=255)
                               cGGTTACACGTTCGTTACCCATCAGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAcgc                                                          <  2:3378562/93‑1 (MQ=255)
                                ggTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAAcgcg                                                         >  1:2156355/1‑93 (MQ=255)
                                ggTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGATTCTGACCGATAGAACCCGGAAcgcg                                                         <  2:837441/93‑1 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        >  2:14780/1‑93 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        >  1:3011891/1‑93 (MQ=255)
                                 gTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCgg                                                        <  1:1247884/93‑1 (MQ=255)
                                   tACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTg                                                      <  2:168466/93‑1 (MQ=255)
                                     cacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgag                                                    >  2:1233084/1‑93 (MQ=255)
                                     cacGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgag                                                    <  2:1086742/93‑1 (MQ=255)
                                      acGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTgaga                                                   >  2:1134850/1‑93 (MQ=255)
                                       cgttctttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTCCTGACCGATAGACCCCGGAACGCGGTGAGAc                                                  <  2:2955086/93‑1 (MQ=255)
                                          tcgttACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAg                                               <  1:3429947/93‑1 (MQ=255)
                                                 ccATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAc                                        <  2:2381661/93‑1 (MQ=255)
                                                  cATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTAcc                                       >  2:3876177/1‑93 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     <  1:964910/93‑1 (MQ=255)
                                                    tCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGt                                     >  2:21573/1‑93 (MQ=255)
                                                     cTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTg                                    <  1:2021159/93‑1 (MQ=255)
                                                      tGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGa                                   >  1:2412664/1‑93 (MQ=255)
                                                      tGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGa                                   >  1:861017/1‑93 (MQ=255)
                                                       gACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAg                                  <  1:45447/93‑1 (MQ=255)
                                                        aCCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGt                                 >  2:3628351/1‑93 (MQ=255)
                                                         ccTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTa                                >  2:3270134/1‑93 (MQ=255)
                                                            gCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTGTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCg                             <  1:115581/93‑1 (MQ=255)
                                                            gCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCg                             >  1:3206523/1‑93 (MQ=255)
                                                              cATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTc                           >  2:732145/1‑93 (MQ=255)
                                                               aTTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGtac                          >  1:1510577/1‑91 (MQ=255)
                                                               aTTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTcc                          >  2:1418789/1‑93 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         <  1:1701110/93‑1 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         <  2:1261840/93‑1 (MQ=255)
                                                                ttttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCt                         >  2:3340808/1‑93 (MQ=255)
                                                                 tttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTg                        <  1:113179/93‑1 (MQ=255)
                                                                  ttCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTgg                       <  2:2790322/93‑1 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  2:541879/1‑93 (MQ=255)
                                                                   tCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTggg                      >  1:2249375/1‑93 (MQ=255)
                                                                    cTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGt                     <  1:2164783/93‑1 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGTACGCGGTGAGACTAGGAGTTACCTATAGTAGCGTCCTGGGTa                    >  1:4214775/1‑93 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTa                    >  1:1023091/1‑93 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTa                    >  2:1317225/1‑93 (MQ=255)
                                                                     ttGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTa                    >  1:3399004/1‑93 (MQ=255)
                                                                      tGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTAc                   <  2:22741/93‑1 (MQ=255)
                                                                       gCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACg                  <  2:3519438/93‑1 (MQ=255)
                                                                         cTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGa                >  2:1592451/1‑93 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               <  1:4502871/93‑1 (MQ=255)
                                                                          tttGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGaa               >  2:4474073/1‑93 (MQ=255)
                                                                             gAACACATTGCCCAGGGACTTGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGtt            <  2:4212014/93‑1 (MQ=255)
                                                                              aaCACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTc           <  1:1453622/93‑1 (MQ=255)
                                                                               acacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTcc          <  1:3080395/93‑1 (MQ=255)
                                                                                cacTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCa         >  1:2486203/1‑93 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        >  2:3766419/1‑93 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        <  2:480279/93‑1 (MQ=255)
                                                                                 acTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAg        >  2:3472128/1‑93 (MQ=255)
                                                                                  cTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTCCCGTGAGTAGCGTCCT‑GGTACGGAAGTTCCAgcg      <  2:719690/93‑1 (MQ=255)
                                                                                   tttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcg      <  1:4155921/93‑1 (MQ=255)
                                                                                   tttGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAgcg      >  1:4718020/1‑93 (MQ=255)
                                                                                     tGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCt    <  2:4131319/93‑1 (MQ=255)
                                                                                      gCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCtt   <  2:4343737/93‑1 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  <  1:631687/93‑1 (MQ=255)
                                                                                       cccAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTa  >  1:4468684/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA  >  NC_000913/3450019‑3450198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450016‑3450202
                                                                                             |                                                                                             
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCA                                                                                               <  SRR4302544.2057372/94‑1 (MQ=60)
 CAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAG                                                                                              <  SRR4302544.2086740/94‑1 (MQ=60)
  AGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302544.3167203/1‑94 (MQ=60)
  AGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302544.3089755/1‑94 (MQ=60)
  AGCGTCAACGCGTACTACGTCAAGGCTCTGAAAGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGA                                                                                             >  SRR4302544.4149549/1‑94 (MQ=60)
   GCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAG                                                                                            <  SRR4302544.1126453/94‑1 (MQ=60)
    CGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGT                                                                                           >  SRR4302544.267615/1‑94 (MQ=60)
     GTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          <  SRR4302544.2474012/94‑1 (MQ=60)
     GTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTC                                                                                          >  SRR4302544.2398469/1‑94 (MQ=60)
      TCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCT                                                                                         >  SRR4302544.1223005/1‑94 (MQ=60)
       CAACGCGTACTACGTCAAGGCTCTGAACGGTTCCACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTG                                                                                        <  SRR4302544.1678089/94‑1 (MQ=60)
       CAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTG                                                                                        >  SRR4302544.2121316/1‑94 (MQ=60)
        AACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTTTTGCCTTTGAACACTTTGCCCACAGTCTGG                                                                                       >  SRR4302544.3885747/1‑94 (MQ=60)
        AACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGG                                                                                       <  SRR4302544.410859/94‑1 (MQ=60)
         ACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGT                                                                                      <  SRR4302544.392445/94‑1 (MQ=60)
          CGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTT                                                                                     >  SRR4302544.1473572/1‑94 (MQ=60)
           GCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTC                                                                                    >  SRR4302544.1931136/1‑94 (MQ=60)
           GCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTC                                                                                    <  SRR4302544.4204924/94‑1 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTATACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCT                                                                                   >  SRR4302544.1572595/1‑94 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   >  SRR4302544.2781909/1‑94 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   <  SRR4302544.4686510/94‑1 (MQ=60)
            CGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCATTGAACACTTTGCCCAGAGTCTGGTTCT                                                                                   >  SRR4302544.855087/1‑94 (MQ=60)
             GTACTACGTCAAGGCTCTTAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  <  SRR4302544.2778554/94‑1 (MQ=60)
             GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302544.4473174/1‑94 (MQ=60)
             GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302544.686139/1‑94 (MQ=60)
             GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302544.493946/1‑94 (MQ=60)
             GTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTG                                                                                  >  SRR4302544.1329045/1‑94 (MQ=60)
               ACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGAC                                                                                <  SRR4302544.4418344/94‑1 (MQ=60)
                CTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACC                                                                               <  SRR4302544.3952821/94‑1 (MQ=60)
                 TACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCG                                                                              <  SRR4302544.1876765/94‑1 (MQ=60)
                 TACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCG                                                                              >  SRR4302544.21575/1‑94 (MQ=60)
                   CGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGAT                                                                            <  SRR4302544.2330464/94‑1 (MQ=60)
                    GTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATA                                                                           >  SRR4302544.2314408/1‑94 (MQ=60)
                    GTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATA                                                                           >  SRR4302544.2515733/1‑94 (MQ=60)
                     TCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAG                                                                          <  SRR4302544.2627580/94‑1 (MQ=60)
                      CAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGA                                                                         <  SRR4302544.2517272/94‑1 (MQ=60)
                       AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302544.1700206/1‑94 (MQ=60)
                       AAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAA                                                                        >  SRR4302544.2183654/1‑94 (MQ=60)
                          GCTCCGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCC                                                                     <  SRR4302544.2770963/94‑1 (MQ=60)
                           CTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCG                                                                    >  SRR4302544.653028/1‑94 (MQ=60)
                           CTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCCATAGCACCCG                                                                    >  SRR4302544.3921880/1‑94 (MQ=60)
                            TCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGG                                                                   <  SRR4302544.821751/94‑1 (MQ=60)
                            TCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGG                                                                   <  SRR4302544.1397004/94‑1 (MQ=60)
                             CTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGA                                                                  >  SRR4302544.470923/1‑94 (MQ=60)
                             CTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGA                                                                  >  SRR4302544.2589943/1‑94 (MQ=60)
                              TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 >  SRR4302544.3792553/1‑94 (MQ=60)
                              TGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAA                                                                 <  SRR4302544.3582540/94‑1 (MQ=60)
                               GAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAAC                                                                <  SRR4302544.4553733/94‑1 (MQ=60)
                                AACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACG                                                               <  SRR4302544.3830106/94‑1 (MQ=60)
                                  CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCG                                                             >  SRR4302544.2176850/1‑94 (MQ=60)
                                  CGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCG                                                             <  SRR4302544.4051209/94‑1 (MQ=60)
                                   GGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGG                                                            >  SRR4302544.2403775/1‑94 (MQ=60)
                                   GGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGG                                                            >  SRR4302544.3040757/1‑94 (MQ=60)
                                    GTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGT                                                           >  SRR4302544.3457408/1‑94 (MQ=60)
                                    GTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGT                                                           <  SRR4302544.1260172/94‑1 (MQ=60)
                                     TTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTG                                                          <  SRR4302544.16017/94‑1 (MQ=60)
                                       tcactTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAG                                                        <  SRR4302544.1941222/89‑1 (MQ=60)
                                       ACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAG                                                        <  SRR4302544.2946850/94‑1 (MQ=60)
                                        CACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGA                                                       >  SRR4302544.473951/1‑94 (MQ=60)
                                           GTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAA                                                    >  SRR4302544.1004767/1‑94 (MQ=60)
                                            TTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAG                                                   >  SRR4302544.452861/1‑94 (MQ=60)
                                             TCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGG                                                  <  SRR4302544.3462731/94‑1 (MQ=60)
                                                TTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGT                                               >  SRR4302544.247910/1‑94 (MQ=60)
                                                  ACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTA                                             >  SRR4302544.2408158/1‑94 (MQ=60)
                                                  ACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTA                                             <  SRR4302544.478361/94‑1 (MQ=60)
                                                    CCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACC                                           <  SRR4302544.2209435/94‑1 (MQ=60)
                                                     CATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCG                                          <  SRR4302544.156787/94‑1 (MQ=60)
                                                      ATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGT                                         <  SRR4302544.2724654/94‑1 (MQ=60)
                                                       TCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTG                                        <  SRR4302544.974381/94‑1 (MQ=60)
                                                        CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       >  SRR4302544.869536/1‑94 (MQ=60)
                                                        CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       <  SRR4302544.2040771/94‑1 (MQ=60)
                                                        CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       >  SRR4302544.2435607/1‑94 (MQ=60)
                                                        CTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGA                                       >  SRR4302544.595479/1‑94 (MQ=60)
                                                         TGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAG                                      >  SRR4302544.3155649/1‑94 (MQ=60)
                                                          GACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGT                                     <  SRR4302544.45899/94‑1 (MQ=60)
                                                              TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCG                                 >  SRR4302544.3237109/1‑94 (MQ=60)
                                                              TGCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCG                                 >  SRR4302544.3237110/1‑94 (MQ=60)
                                                               GCCATTTTCTTGCCTTTGAACACTTTGCCCAGAGTGTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGT                                <  SRR4302544.116635/94‑1 (MQ=60)
                                                                 CATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCC                              <  SRR4302544.183559/94‑1 (MQ=60)
                                                                 CATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTAC                              >  SRR4302544.1525550/1‑94 (MQ=60)
                                                                 CATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGATTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCC                              >  SRR4302544.1211215/1‑94 (MQ=60)
                                                                  ATTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCT                             >  SRR4302544.4099479/1‑94 (MQ=60)
                                                                   TTTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTG                            <  SRR4302544.1717896/94‑1 (MQ=60)
                                                                    TTTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGG                           <  SRR4302544.114212/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACGCTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACTCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302544.4156557/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          <  SRR4302544.2440949/94‑1 (MQ=60)
                                                                     TTCTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGG                          >  SRR4302544.2270513/1‑94 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGTACGCGGTGAGACTAGGAGTTACCTATAGTAGCGTCCTGGGTA                        >  SRR4302544.4257305/1‑94 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        <  SRR4302544.2185333/94‑1 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        <  SRR4302544.2336292/94‑1 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        >  SRR4302544.1033309/1‑94 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        >  SRR4302544.1466212/1‑94 (MQ=60)
                                                                       CTTGCCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTA                        >  SRR4302544.3431505/1‑94 (MQ=60)
                                                                           CCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGA                    >  SRR4302544.2526104/1‑94 (MQ=60)
                                                                           CCTTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGACACAAGGAGTTACCGGGAGTAGCGCCCTGGGCAGGGA                    >  SRR4302544.3642854/1‑94 (MQ=60)
                                                                            CTTTGAACACTTTGCCCAGAGGCTGGTTCTGACAGATAGAACCCGGCACGCGGTGAGACAAGGAGTTAGCGTGAGAAGATCCCTGGGTACGGAA                   >  SRR4302544.4324515/1‑94 (MQ=55)
                                                                             TTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAG                  <  SRR4302544.2162490/94‑1 (MQ=60)
                                                                             TTTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAG                  <  SRR4302544.4548043/94‑1 (MQ=60)
                                                                              TTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGT                 <  SRR4302544.3871002/94‑1 (MQ=60)
                                                                              TTGAACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGT                 >  SRR4302544.1158514/1‑94 (MQ=60)
                                                                                 AACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCC              <  SRR4302544.1468066/94‑1 (MQ=60)
                                                                                  ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             <  SRR4302544.3109735/94‑1 (MQ=60)
                                                                                  ACACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCA             >  SRR4302544.2510019/1‑94 (MQ=60)
                                                                                   atCTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            <  SRR4302544.3822272/92‑1 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            >  SRR4302544.1711710/1‑94 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            >  SRR4302544.1227373/1‑94 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            >  SRR4302544.1903144/1‑94 (MQ=60)
                                                                                   CACTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAG            <  SRR4302544.875716/94‑1 (MQ=60)
                                                                                     CTTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCG          >  SRR4302544.4764621/1‑94 (MQ=60)
                                                                                      TTTGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGC         <  SRR4302544.4197087/94‑1 (MQ=60)
                                                                                        TGCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGAGTCCTGGGTACGGAAGTTCCAGCGCTT       >  SRR4302544.3559020/1‑94 (MQ=60)
                                                                                         GCCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTA      >  SRR4302544.4513631/1‑94 (MQ=60)
                                                                                          CCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAA     <  SRR4302544.637910/94‑1 (MQ=60)
                                                                                          CCCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAA     >  SRR4302544.290532/1‑94 (MQ=60)
                                                                                           CCAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAAC    <  SRR4302544.2469177/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGACCCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302544.908817/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGTAGGTACCGTGATTAGGGTCTGGGGTAAGGAAGTTCCAGCCCTTAACG   >  SRR4302544.1027593/1‑94 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302544.908816/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   <  SRR4302544.1824272/94‑1 (MQ=60)
                                                                                            CAGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGCGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACG   >  SRR4302544.1027738/1‑94 (MQ=60)
                                                                                             AGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCCGCGCTTAACGG  >  SRR4302544.4605011/1‑94 (MQ=60)
                                                                                             |                                                                                             
TCAGCGTCAACGCGTACTACGTCAAGGCTCTGAACGGTTACACGTTCGTTACCCATCTGACCTGCCATTTTCTTGCCTTTGAACACTTTGCCCGGAGTCTGGTTCTGACCGATAGAACCCGGAACGCGGTGAGACAAGGAGTTACCGTGAGTAGCGTCCTGGGTACGGAAGTTCCAGCGCTTAACGG  >  NC_000913/3450016‑3450202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑