Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,456,098 A→G Q41Q (CAA→CAG)  yheG → YheG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,456,0980AG98.8% 545.7 / ‑2.8 170Q41Q (CAA→CAG) yheGYheG
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (79/89);  total (79/91)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 178 total aligned reads displayed.

GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456008‑3456187
                                                                                          |                                                                                         
ggCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCg                                                                                         <  1:4667046/93‑1 (MQ=255)
  cgcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTc                                                                                       <  2:4145843/93‑1 (MQ=255)
   gcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCa                                                                                      <  1:3850570/93‑1 (MQ=255)
   gcTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCa                                                                                      <  1:2736349/93‑1 (MQ=255)
     tGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAgg                                                                                    >  1:244705/1‑93 (MQ=255)
     tGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAgg                                                                                    >  2:1801055/1‑93 (MQ=255)
      ggCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGt                                                                                   >  1:2171626/1‑93 (MQ=255)
       gCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGtt                                                                                  <  2:4519246/93‑1 (MQ=255)
         acaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgg                                                                                <  1:4331128/93‑1 (MQ=255)
         acaACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgg                                                                                >  1:4485288/1‑93 (MQ=255)
         acaACGAATTGGTTATCTCTATTCAGACGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTgc                                                                                >  1:4096315/1‑92 (MQ=255)
          caACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGc                                                                               >  1:2753217/1‑93 (MQ=255)
          caACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAATTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGGTGGc                                                                               >  1:792617/1‑93 (MQ=255)
           aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                              <  2:4591310/93‑1 (MQ=255)
           aaCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGcc                                                                              >  1:4458427/1‑93 (MQ=255)
            aCGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCa                                                                             <  1:4274421/93‑1 (MQ=255)
             agAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAg                                                                            <  1:3927280/92‑1 (MQ=255)
              gAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGt                                                                           <  2:838021/93‑1 (MQ=255)
              gAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGt                                                                           <  2:1123937/93‑1 (MQ=255)
              gAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGt                                                                           >  2:1702135/1‑93 (MQ=255)
                 ttGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGcc                                                                        >  1:1161171/1‑93 (MQ=255)
                 ttGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGcc                                                                        <  2:2486913/93‑1 (MQ=255)
                  tGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCa                                                                       <  2:1422744/93‑1 (MQ=255)
                   ggTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAg                                                                      >  1:1075066/1‑93 (MQ=255)
                   ggTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAg                                                                      <  2:1560039/93‑1 (MQ=255)
                        tctcTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAt                                                                 >  2:4128717/1‑93 (MQ=255)
                         ctctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATg                                                                <  2:1657468/93‑1 (MQ=255)
                           ctATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAc                                                              >  1:1064124/1‑93 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             >  2:470397/1‑93 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             >  2:3973036/1‑93 (MQ=255)
                            tATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAcc                                                             <  2:2953914/93‑1 (MQ=255)
                             aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCa                                                            <  1:1475188/93‑1 (MQ=255)
                             aTTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCa                                                            >  1:4584773/1‑93 (MQ=255)
                              ttCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAg                                                           <  2:4089183/93‑1 (MQ=255)
                              tgcAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAg                                                           <  1:1857299/91‑1 (MQ=255)
                                cAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCt                                                         <  2:173640/93‑1 (MQ=255)
                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        <  2:3604171/93‑1 (MQ=255)
                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        >  2:1300071/1‑93 (MQ=255)
                                 agagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCAATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCtt                                                        >  1:1657904/1‑93 (MQ=255)
                                  gagGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCAAGTGCCAGACCATGACCAGCTTg                                                       >  1:993804/1‑93 (MQ=255)
                                    gggCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTc                                                     <  1:891190/93‑1 (MQ=255)
                                      gCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTcac                                                   >  2:422696/1‑93 (MQ=255)
                                       ggagaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGGCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTcaca                                                  <  1:4002358/92‑1 (MQ=255)
                                         agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                                <  2:4389811/93‑1 (MQ=255)
                                         agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                                <  2:4244734/93‑1 (MQ=255)
                                         agaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAgg                                                <  2:3367873/93‑1 (MQ=255)
                                          gaTTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGc                                               <  1:3249893/93‑1 (MQ=255)
                                           attattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              <  2:4253786/93‑1 (MQ=255)
                                           attattTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCt                                              <  2:3199123/93‑1 (MQ=255)
                                            ttatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTg                                             >  2:1469890/1‑93 (MQ=255)
                                             tatttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTgt                                            >  1:3548122/1‑92 (MQ=255)
                                               tttatCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGAtt                                          <  2:3185655/93‑1 (MQ=255)
                                                  atCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTg                                       <  2:2282870/93‑1 (MQ=255)
                                                   tctcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGa                                      <  1:2935313/93‑1 (MQ=255)
                                                    ctcGCCGATGCGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGaa                                     <  1:3292360/93‑1 (MQ=255)
                                                     tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAg                                    >  1:2743675/1‑93 (MQ=255)
                                                     tcGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAg                                    >  2:1533193/1‑93 (MQ=255)
                                                      cGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGCTTTTGAAGc                                   >  2:1216094/1‑93 (MQ=255)
                                                      cGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGc                                   <  2:2860723/93‑1 (MQ=255)
                                                        ccGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTc                                 >  1:4193683/1‑93 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               <  2:4287298/93‑1 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  1:3243517/1‑93 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgg                               >  2:2884416/1‑93 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCgc                               >  1:3667999/1‑92 (MQ=255)
                                                          gATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGCCACAGGCTGATTTTGAAGCCCgg                               >  2:3441074/1‑93 (MQ=255)
                                                            tACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCGCAGGCTGATTTTGAAGCTCGGCt                             >  2:2616997/1‑93 (MQ=255)
                                                             aCGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTg                            <  1:635687/93‑1 (MQ=255)
                                                              cGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTgg                           >  1:4712033/1‑93 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  1:967562/93‑1 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         >  1:4159106/1‑93 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  2:17437/93‑1 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         <  1:2444821/93‑1 (MQ=255)
                                                                ccATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGaa                         >  1:1951136/1‑93 (MQ=255)
                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAg                        >  2:3670048/1‑93 (MQ=255)
                                                                 cATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAg                        >  2:750881/1‑93 (MQ=255)
                                                                   ttCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCg                      <  1:4550294/93‑1 (MQ=255)
                                                                   ttCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCg                      >  1:3497883/1‑93 (MQ=255)
                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                    <  1:2636633/93‑1 (MQ=255)
                                                                     cGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGt                    <  1:1571072/93‑1 (MQ=255)
                                                                      gAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTa                   <  1:2143894/93‑1 (MQ=255)
                                                                       ggCGGTTACTCGATATTCAGCGTGAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTGGGCTGGAAGCGGTAt                  <  1:3786939/92‑1 (MQ=255)
                                                                       aGCGGTTACTCGATATTCAGCGTCAGGTTTGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGGAt                  >  2:2549098/1‑93 (MQ=255)
                                                                       aGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAt                  >  1:701284/1‑93 (MQ=255)
                                                                          ggTTGCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTcc               <  2:4035061/93‑1 (MQ=255)
                                                                          ggTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTcc               <  2:3386358/93‑1 (MQ=255)
                                                                          ggTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTcc               <  2:2946517/93‑1 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              >  1:2014417/1‑93 (MQ=255)
                                                                           gTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCa              >  2:2244692/1‑93 (MQ=255)
                                                                              aCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCa           <  2:3641519/93‑1 (MQ=255)
                                                                              aCTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCa           <  2:1932280/93‑1 (MQ=255)
                                                                               cTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAg          >  1:4744809/1‑93 (MQ=255)
                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       <  1:2535737/93‑1 (MQ=255)
                                                                                  gATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAAt       >  2:3748312/1‑93 (MQ=255)
                                                                                  cagatTCACCTTCACGTTGGCCAGTTCCAGACCATGGCCAGCTTGTCACAGGCTGATTTTGACGCTCGGCTGGAAGCGGTATTCCATAAGAAt       <  2:3681256/90‑1 (MQ=255)
                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGTTCGGCTGGAAGCGGTATTCCATCAGAATa      <  2:2757813/93‑1 (MQ=255)
                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      >  1:141910/1‑93 (MQ=255)
                                                                                   atatTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATa      <  2:2757812/93‑1 (MQ=255)
                                                                                     atTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAcc    <  2:1172783/93‑1 (MQ=255)
                                                                                     atTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAcc    >  2:4621124/1‑93 (MQ=255)
                                                                                       tCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCgg  <  1:1489727/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGG  >  NC_000913/3456008‑3456187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA  >  NC_000913/3456008‑3456190
                                                                                          |                                                                                            
GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGT                                                                                           <  SRR4302544.4713195/94‑1 (MQ=60)
   GCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAG                                                                                        <  SRR4302544.2762725/94‑1 (MQ=60)
   GCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAG                                                                                        <  SRR4302544.3888791/94‑1 (MQ=60)
    CTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGG                                                                                       >  SRR4302544.247259/1‑94 (MQ=60)
    CTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGG                                                                                       <  SRR4302544.1942837/94‑1 (MQ=60)
     TGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGT                                                                                      >  SRR4302544.2192217/1‑94 (MQ=60)
      GGCACAACGAATTGGTTATCTCTATACAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTT                                                                                     <  SRR4302544.4222914/94‑1 (MQ=60)
        CACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGG                                                                                   >  SRR4302544.4293732/1‑94 (MQ=60)
        CACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGG                                                                                   >  SRR4302544.4530348/1‑94 (MQ=60)
        CACAACGAATTGGTTATCTCTATTCAGACGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGC                                                                                   >  SRR4302544.4137081/1‑94 (MQ=60)
         ACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGC                                                                                  <  SRR4302544.4375229/94‑1 (MQ=60)
         ACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGC                                                                                  >  SRR4302544.2779724/1‑94 (MQ=60)
         ACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAATTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGGTGGC                                                                                  >  SRR4302544.800289/1‑94 (MQ=60)
          CAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCC                                                                                 >  SRR4302544.4503313/1‑94 (MQ=60)
          CAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCAATTCGAGCGGTTACTCGATATTCAGCGTAAGGTTGGCA                                                                                 >  SRR4302544.3405229/1‑94 (MQ=60)
           AACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTtgccc                                                                                >  SRR4302544.2868642/1‑89 (MQ=60)
            ACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAG                                                                               <  SRR4302544.4318062/94‑1 (MQ=60)
             CGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGT                                                                              >  SRR4302544.780377/1‑94 (MQ=60)
             AGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGT                                                                              <  SRR4302544.3966283/94‑1 (MQ=60)
                ATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCC                                                                           >  SRR4302544.1172742/1‑94 (MQ=60)
                  TGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAG                                                                         >  SRR4302544.1085757/1‑94 (MQ=60)
                   GGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGA                                                                        >  SRR4302544.996150/1‑94 (MQ=60)
                       ATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCAT                                                                    <  SRR4302544.678399/94‑1 (MQ=60)
                          TCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGAC                                                                 >  SRR4302544.1074703/1‑94 (MQ=60)
                            TATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCA                                                               >  SRR4302544.4630419/1‑94 (MQ=60)
                             ATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAG                                                              <  SRR4302544.1489783/94‑1 (MQ=60)
                             ATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAG                                                              <  SRR4302544.1623132/94‑1 (MQ=60)
                              TGCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGC                                                             <  SRR4302544.1875259/94‑1 (MQ=60)
                                CAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTT                                                           >  SRR4302544.2545843/1‑94 (MQ=60)
                                CAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCAATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTT                                                           >  SRR4302544.1674238/1‑94 (MQ=60)
                                 AGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCAAGTGCCAGACCATGACCAGCTTG                                                          >  SRR4302544.1003631/1‑94 (MQ=60)
                                    gtgctaaATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTACCAGACCATGACCAGCTTGTCA                                                       <  SRR4302544.2244635/87‑1 (MQ=60)
                                    GGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCA                                                       <  SRR4302544.2244634/94‑1 (MQ=60)
                                    GGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCA                                                       <  SRR4302544.900017/94‑1 (MQ=60)
                                    GGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCA                                                       <  SRR4302544.900016/94‑1 (MQ=60)
                                    GGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCA                                                       <  SRR4302544.4597380/94‑1 (MQ=60)
                                      GCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACA                                                     >  SRR4302544.1542204/1‑94 (MQ=60)
                                       GGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGGCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAG                                                    <  SRR4302544.4042191/94‑1 (MQ=60)
                                       CGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAG                                                    >  SRR4302544.2615593/1‑94 (MQ=60)
                                         AGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACCGGC                                                  >  SRR4302544.2116396/1‑94 (MQ=60)
                                          GATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCT                                                 <  SRR4302544.1471530/94‑1 (MQ=60)
                                          GATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCT                                                 <  SRR4302544.3280988/94‑1 (MQ=60)
                                           ATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTG                                                <  SRR4302544.4080003/94‑1 (MQ=60)
                                            TTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGT                                               >  SRR4302544.3582101/1‑94 (MQ=60)
                                               TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTT                                            >  SRR4302544.4020151/1‑94 (MQ=60)
                                               TTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTT                                            <  SRR4302544.4710876/94‑1 (MQ=60)
                                                   TCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAA                                        <  SRR4302544.2963510/94‑1 (MQ=60)
                                                    CTCGCCGATGCGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                       <  SRR4302544.3323724/94‑1 (MQ=60)
                                                    CTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAG                                       >  SRR4302544.2770115/1‑94 (MQ=60)
                                                     TCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGC                                      <  SRR4302544.2769872/94‑1 (MQ=60)
                                                       GCCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTC                                    >  SRR4302544.4235227/1‑94 (MQ=60)
                                                        CCGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCG                                   <  SRR4302544.3892544/94‑1 (MQ=60)
                                                         CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                  >  SRR4302544.3274569/1‑94 (MQ=60)
                                                         CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGG                                  >  SRR4302544.3274568/1‑94 (MQ=60)
                                                         CGATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGC                                  >  SRR4302544.3704577/1‑94 (MQ=60)
                                                          GATACGCCATTCGAGCGGTTACTCGATATTTAGCGTCAGGTTGGCCAGTGCCAGACCATGACCGGCTTGTCACAGGCTGATTTTGAAGCTCGGC                                 >  SRR4302544.1125719/1‑94 (MQ=60)
                                                           ATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCT                                <  SRR4302544.4574172/94‑1 (MQ=60)
                                                           ATACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCT                                <  SRR4302544.505455/94‑1 (MQ=60)
                                                             ACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGG                              <  SRR4302544.641954/94‑1 (MQ=60)
                                                             ACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGG                              >  SRR4302544.4758584/1‑94 (MQ=60)
                                                             ACGCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGG                              >  SRR4302544.4758583/1‑94 (MQ=60)
                                                               GCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAA                            >  SRR4302544.4200303/1‑94 (MQ=60)
                                                               GCCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAA                            >  SRR4302544.1970053/1‑94 (MQ=60)
                                                                CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                           <  SRR4302544.2468127/94‑1 (MQ=60)
                                                                CCATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAG                           <  SRR4302544.977048/94‑1 (MQ=60)
                                                                  ATTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCG                         >  SRR4302544.3531240/1‑94 (MQ=60)
                                                                   TTCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGG                        <  SRR4302544.4595727/94‑1 (MQ=60)
                                                                    TCGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGT                       <  SRR4302544.4454622/94‑1 (MQ=60)
                                                                     CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                      <  SRR4302544.1586617/94‑1 (MQ=60)
                                                                     CGAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTA                      <  SRR4302544.2661908/94‑1 (MQ=60)
                                                                      GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                     <  SRR4302544.2164340/94‑1 (MQ=60)
                                                                      GAGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTAT                     >  SRR4302544.708266/1‑94 (MQ=60)
                                                                       GGCGGTTACTCGATATTCAGCGTGAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTGGGCTGGAAGCGGTATT                    <  SRR4302544.3824438/94‑1 (MQ=60)
                                                                       AGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATT                    <  SRR4302544.3824437/94‑1 (MQ=60)
                                                                       AGCGGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATT                    <  SRR4302544.1323309/94‑1 (MQ=60)
                                                                          GGTTACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCA                 >  SRR4302544.2033991/1‑94 (MQ=60)
                                                                             TACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCA              <  SRR4302544.3189747/94‑1 (MQ=60)
                                                                              ACTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAG             >  SRR4302544.4791562/1‑94 (MQ=60)
                                                                              ACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGTCCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAG             <  SRR4302544.4325751/94‑1 (MQ=60)
                                                                               CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGA            >  SRR4302544.2688120/1‑94 (MQ=60)
                                                                               CTCGATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGA            >  SRR4302544.1852199/1‑94 (MQ=60)
                                                                                  GATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATA         <  SRR4302544.2560092/94‑1 (MQ=60)
                                                                                  GATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATA         >  SRR4302544.143252/1‑94 (MQ=60)
                                                                                   ATATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATAC        >  SRR4302544.3539140/1‑94 (MQ=60)
                                                                                    TATTCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACC       <  SRR4302544.369771/94‑1 (MQ=60)
                                                                                       TCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGT    <  SRR4302544.2177637/94‑1 (MQ=60)
                                                                                       TCAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGT    <  SRR4302544.1504454/94‑1 (MQ=60)
                                                                                        CAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTG   <  SRR4302544.797541/94‑1 (MQ=60)
                                                                                        CAGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTG   >  SRR4302544.3112243/1‑94 (MQ=60)
                                                                                         AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA  >  SRR4302544.184498/1‑94 (MQ=60)
                                                                                         AGCGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA  >  SRR4302544.2060634/1‑94 (MQ=60)
                                                                                          |                                                                                            
GGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTATTTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGTCACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGA  >  NC_000913/3456008‑3456190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑