Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,472,167 A→C V8G (GTA→GGA)  rpsL ← 30S ribosomal subunit protein S12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,472,1670AC98.2% 704.5 / ‑1.0 218V8G (GTA→GGA) rpsL30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base C (80/134);  total (82/136)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 229 total aligned reads displayed.

TATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGG  >  NC_000913/3472077‑3472256
                                                                                          |                                                                                         
tatcCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                         <  1:334536/93‑1 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                         <  2:2019617/93‑1 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                         <  1:2715853/93‑1 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                         >  1:2765222/1‑93 (MQ=255)
tataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTccc                                                                                         <  2:1240059/93‑1 (MQ=255)
 ataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCa                                                                                        <  2:1660554/93‑1 (MQ=255)
 ataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCa                                                                                        >  2:59986/1‑93 (MQ=255)
 ataCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCa                                                                                        <  1:3059351/93‑1 (MQ=255)
  taCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAg                                                                                       <  1:538264/93‑1 (MQ=255)
   acacGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGc                                                                                      >  2:3842037/1‑93 (MQ=255)
    cacGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCt                                                                                     >  2:2743435/1‑93 (MQ=255)
     acGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTg                                                                                    >  1:2085311/1‑93 (MQ=255)
       gAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGt                                                                                  <  1:2348153/93‑1 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                 <  2:1860528/93‑1 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                 <  1:1012114/93‑1 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                 <  1:2812650/93‑1 (MQ=255)
        aGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGtt                                                                                 <  1:1198608/93‑1 (MQ=255)
          tACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTaa                                                                               >  2:1143007/1‑93 (MQ=255)
          tACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTaa                                                                               >  1:3894296/1‑93 (MQ=255)
           aCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAAc                                                                              <  2:535572/93‑1 (MQ=255)
           aCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAAc                                                                              >  2:4682411/1‑93 (MQ=255)
            cATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACt                                                                             <  2:2524216/93‑1 (MQ=255)
            cATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACt                                                                             <  1:1496600/93‑1 (MQ=255)
              tACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGt                                                                           <  2:3291779/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                          <  1:4089661/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                          <  2:271600/93‑1 (MQ=255)
               aCGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGtt                                                                          <  2:1389072/93‑1 (MQ=255)
                 gCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGc                                                                        <  1:3933181/93‑1 (MQ=255)
                  ccACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGcc                                                                       <  2:3932709/93‑1 (MQ=255)
                  ccACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGcc                                                                       <  1:4662669/93‑1 (MQ=255)
                  ccACGTATTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGcc                                                                       >  2:4607080/1‑93 (MQ=255)
                   cACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCa                                                                      <  2:3485423/93‑1 (MQ=255)
                   cACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCa                                                                      <  2:3629321/93‑1 (MQ=255)
                    aCGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCAt                                                                     >  2:2119869/1‑93 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                 <  1:3963535/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                 <  1:167802/93‑1 (MQ=255)
                        ttttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTaaa                                                                 <  1:1397238/93‑1 (MQ=255)
                          ttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATa                                                               <  2:2875818/93‑1 (MQ=255)
                          ttGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATa                                                               <  2:337502/93‑1 (MQ=255)
                               ggCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTcc                                                          <  1:3780078/93‑1 (MQ=255)
                               ggCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTcc                                                          <  1:1899145/93‑1 (MQ=255)
                                gCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCt                                                         <  2:4291165/93‑1 (MQ=255)
                                  aTGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTgg                                                       >  2:3636543/1‑93 (MQ=255)
                                   tGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGt                                                      >  2:4227899/1‑93 (MQ=255)
                                   tGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGt                                                      >  1:1332843/1‑93 (MQ=255)
                                   tGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGt                                                      <  1:2862535/93‑1 (MQ=255)
                                      ttCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGtttt                                                   >  2:4109451/1‑93 (MQ=255)
                                       tCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTa                                                  <  1:4003679/93‑1 (MQ=255)
                                          aGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCt                                               >  1:424948/1‑93 (MQ=255)
                                           gcgcAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCtt                                              <  2:171437/93‑1 (MQ=255)
                                           gcgcAGGCACGTTGCTCTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTTTTTCCATTAAATAGCGCCTGGTTTTAGCtt                                              <  2:729948/93‑1 (MQ=255)
                                               aGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGc                                          <  2:2695994/93‑1 (MQ=255)
                                               aGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGc                                          >  2:2045605/1‑93 (MQ=255)
                                                ggCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCt                                         >  2:1795489/1‑93 (MQ=255)
                                                  caagTTGATTTTCGCAGCTTTGCGAGCACATGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCATGGTTTTAGCTTTTGCTTc                                       <  2:4347132/90‑1 (MQ=255)
                                                  cACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTc                                       <  2:1304311/93‑1 (MQ=255)
                                                  cACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTc                                       <  2:2249680/93‑1 (MQ=255)
                                                   aCGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCg                                      >  1:1567109/1‑93 (MQ=255)
                                                     gTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTa                                    >  1:2114724/1‑93 (MQ=255)
                                                     gTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTa                                    >  2:2985318/1‑93 (MQ=255)
                                                       tGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaaa                                  <  2:2322167/93‑1 (MQ=255)
                                                       tGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTaaa                                  >  1:4719565/1‑93 (MQ=255)
                                                        gCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAac                                 >  2:4411239/1‑93 (MQ=255)
                                                         cTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAaca                                >  2:1469507/1‑93 (MQ=255)
                                                          ttttCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTGAacac                               >  2:3876159/1‑93 (MQ=255)
                                                           tttCGCAAATTTGCGAGCTCGTGGTTTGCGTCCCAGCTGGTTAACTGTTCCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACg                              <  2:1224695/93‑1 (MQ=255)
                                                              cGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaa                           <  1:2380727/93‑1 (MQ=255)
                                                              cGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaa                           <  2:3262632/93‑1 (MQ=255)
                                                                 aaCTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaataa                        >  1:1955750/1‑93 (MQ=255)
                                                                 aaCTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaataa                        <  1:3839987/93‑1 (MQ=255)
                                                                 aaCTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaataa                        <  2:3658743/93‑1 (MQ=255)
                                                                   cTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa                      <  2:821184/93‑1 (MQ=255)
                                                                   cTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATaaaa                      <  2:45876/93‑1 (MQ=255)
                                                                    tttGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAc                     <  1:3086904/93‑1 (MQ=255)
                                                                     ttGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACg                    <  2:3755279/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                  <  1:1186666/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                  <  2:3440622/93‑1 (MQ=255)
                                                                       gCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTc                  <  1:4543098/93‑1 (MQ=255)
                                                                        cGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTcc                 >  2:4565902/1‑93 (MQ=255)
                                                                         gAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt                >  1:4303990/1‑93 (MQ=255)
                                                                         gAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCt                <  1:2608210/93‑1 (MQ=255)
                                                                            cACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcac             >  2:2826138/1‑93 (MQ=255)
                                                                              cGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacac           >  1:709247/1‑93 (MQ=255)
                                                                               gTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacaca          >  1:344522/1‑93 (MQ=255)
                                                                               gTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTcacaca          >  1:2502030/1‑93 (MQ=255)
                                                                                tGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACaa         >  2:854738/1‑93 (MQ=255)
                                                                                 ggTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAat        <  2:145581/93‑1 (MQ=255)
                                                                                 ggTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAat        <  2:4004442/93‑1 (MQ=255)
                                                                                  gTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAata       <  2:3247966/93‑1 (MQ=255)
                                                                                  gTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAata       <  2:255522/93‑1 (MQ=255)
                                                                                  gTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAata       >  1:3740474/1‑93 (MQ=255)
                                                                                   tttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAatat      >  1:2224766/1‑93 (MQ=255)
                                                                                    ttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATg     <  1:3301717/93‑1 (MQ=255)
                                                                                    ttGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATg     <  1:2995454/93‑1 (MQ=255)
                                                                                     tGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa    <  2:3142049/93‑1 (MQ=255)
                                                                                     tGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa    <  2:1343886/93‑1 (MQ=255)
                                                                                     tGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGa    <  1:675886/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg   <  2:3340617/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg   <  1:1719226/93‑1 (MQ=255)
                                                                                      gCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAg   <  2:967980/93‑1 (MQ=255)
                                                                                          |                                                                                         
TATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGG  >  NC_000913/3472077‑3472256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAC  >  NC_000913/3472075‑3472258
                                                                                            |                                                                                           
TATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCC                                                                                            >  SRR4302544.2530753/1‑94 (MQ=60)
 ATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCC                                                                                           >  SRR4302544.2791818/1‑94 (MQ=60)
  TATCCACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCA                                                                                          <  SRR4302544.338011/94‑1 (MQ=60)
  TATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCA                                                                                          <  SRR4302544.2741993/94‑1 (MQ=60)
   ATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAG                                                                                         <  SRR4302544.3088548/94‑1 (MQ=60)
    TACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGC                                                                                        <  SRR4302544.543580/94‑1 (MQ=60)
     ACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCT                                                                                       <  SRR4302544.408560/94‑1 (MQ=60)
      CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTG                                                                                      >  SRR4302544.2105366/1‑94 (MQ=60)
      CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTG                                                                                      >  SRR4302544.1374164/1‑94 (MQ=60)
      CACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTG                                                                                      >  SRR4302544.3115142/1‑94 (MQ=60)
         GAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTT                                                                                   <  SRR4302544.2632223/94‑1 (MQ=60)
         GAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTT                                                                                   <  SRR4302544.2370396/94‑1 (MQ=60)
          AGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTA                                                                                  <  SRR4302544.1022200/94‑1 (MQ=60)
          AGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTA                                                                                  <  SRR4302544.2839791/94‑1 (MQ=60)
          AGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTA                                                                                  >  SRR4302544.3459344/1‑94 (MQ=60)
          AGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTA                                                                                  <  SRR4302544.1210485/94‑1 (MQ=60)
           GTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAA                                                                                 >  SRR4302544.3932981/1‑94 (MQ=60)
              CATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTG                                                                              <  SRR4302544.1511400/94‑1 (MQ=60)
               ATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGT                                                                             >  SRR4302544.1586615/1‑94 (MQ=60)
                 ACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTG                                                                           <  SRR4302544.2449021/94‑1 (MQ=60)
                 ACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTG                                                                           <  SRR4302544.4130336/94‑1 (MQ=60)
                 ACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTG                                                                           <  SRR4302544.2121803/94‑1 (MQ=60)
                  CGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGC                                                                          >  SRR4302544.4295797/1‑94 (MQ=60)
                   GCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCC                                                                         <  SRR4302544.3972244/94‑1 (MQ=60)
                    CCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCA                                                                        <  SRR4302544.4708789/94‑1 (MQ=60)
                     CACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCAT                                                                       >  SRR4302544.673264/1‑94 (MQ=60)
                      ACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGAACGTGGTTTGCGTGCCAGCTGGTTAACTGTTGCCATT                                                                      >  SRR4302544.2779690/1‑94 (MQ=60)
                         TTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTTGCAACTTTGCGAGAACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAA                                                                   >  SRR4302544.794021/1‑94 (MQ=60)
                          TTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAAT                                                                  <  SRR4302544.169452/94‑1 (MQ=60)
                          TTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAAT                                                                  <  SRR4302544.1411163/94‑1 (MQ=60)
                          TTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAAT                                                                  <  SRR4302544.4002957/94‑1 (MQ=60)
                            TTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAG                                                                <  SRR4302544.1160039/94‑1 (MQ=60)
                              GCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCT                                                              <  SRR4302544.3838857/94‑1 (MQ=60)
                               CGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTC                                                             <  SRR4302544.1204147/94‑1 (MQ=60)
                                GGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCC                                                            <  SRR4302544.4028881/94‑1 (MQ=60)
                                GGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCC                                                            >  SRR4302544.2873356/1‑94 (MQ=60)
                                 GGCATGCTTCCAGCGCATGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCT                                                           <  SRR4302544.2883930/94‑1 (MQ=60)
                                 GGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCT                                                           <  SRR4302544.4507442/94‑1 (MQ=60)
                                 GGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCT                                                           >  SRR4302544.3909659/1‑94 (MQ=60)
                                 GGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCT                                                           <  SRR4302544.3817518/94‑1 (MQ=60)
                                 GGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCT                                                           <  SRR4302544.1917497/94‑1 (MQ=60)
                                   CATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGG                                                         <  SRR4302544.4076608/94‑1 (MQ=60)
                                    ATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGT                                                        >  SRR4302544.1345968/1‑94 (MQ=60)
                                     TGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTT                                                       <  SRR4302544.2890162/94‑1 (MQ=60)
                                       CTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTT                                                     <  SRR4302544.2395640/94‑1 (MQ=60)
                                       CTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTT                                                     >  SRR4302544.1731880/1‑94 (MQ=60)
                                       CTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTT                                                     >  SRR4302544.3782205/1‑94 (MQ=60)
                                       CTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTT                                                     >  SRR4302544.3758199/1‑94 (MQ=60)
                                        TTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTA                                                    <  SRR4302544.2205470/94‑1 (MQ=60)
                                         TCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAG                                                   <  SRR4302544.4043522/94‑1 (MQ=60)
                                          CCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGC                                                  >  SRR4302544.4783901/1‑94 (MQ=60)
                                          CCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGC                                                  >  SRR4302544.3161725/1‑94 (MQ=60)
                                          CCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGC                                                  <  SRR4302544.4001430/94‑1 (MQ=60)
                                           CAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCT                                                 >  SRR4302544.429274/1‑94 (MQ=60)
                                            AGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTT                                                <  SRR4302544.3940989/94‑1 (MQ=60)
                                              CGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTT                                              >  SRR4302544.2642208/1‑94 (MQ=60)
                                              CGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTT                                              <  SRR4302544.1979343/94‑1 (MQ=60)
                                               GCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTG                                             >  SRR4302544.624262/1‑94 (MQ=60)
                                                 AGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCT                                           >  SRR4302544.4465960/1‑94 (MQ=60)
                                                 AGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCT                                           >  SRR4302544.2692952/1‑94 (MQ=60)
                                                  GGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTT                                          <  SRR4302544.29881/94‑1 (MQ=60)
                                                    CACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCG                                        >  SRR4302544.1577570/1‑94 (MQ=60)
                                                    CACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCG                                        >  SRR4302544.1582639/1‑94 (MQ=60)
                                                      CGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTA                                      >  SRR4302544.3198930/1‑94 (MQ=60)
                                                      CGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTA                                      >  SRR4302544.2135045/1‑94 (MQ=60)
                                                       GTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAA                                     >  SRR4302544.1964386/1‑94 (MQ=60)
                                                        TTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAA                                    >  SRR4302544.2331357/1‑94 (MQ=60)
                                                        TTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAA                                    >  SRR4302544.4766170/1‑94 (MQ=60)
                                                        TTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAA                                    >  SRR4302544.2331358/1‑94 (MQ=60)
                                                         TGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAAC                                   >  SRR4302544.2829032/1‑94 (MQ=60)
                                                           CTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACAC                                 <  SRR4302544.1610211/94‑1 (MQ=60)
                                                           CTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACAC                                 <  SRR4302544.143245/94‑1 (MQ=60)
                                                            TTTTCGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACG                                <  SRR4302544.3045718/94‑1 (MQ=60)
                                                                CGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAAT                            <  SRR4302544.4751679/94‑1 (MQ=60)
                                                                CGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAAT                            <  SRR4302544.2403268/94‑1 (MQ=60)
                                                                CGCAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAAT                            <  SRR4302544.4641779/94‑1 (MQ=60)
                                                                  CAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAA                          >  SRR4302544.1974722/1‑94 (MQ=60)
                                                                  CAACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAA                          >  SRR4302544.3357291/1‑94 (MQ=60)
                                                                   AACTTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAA                         <  SRR4302544.3878082/94‑1 (MQ=60)
                                                                     CGTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAAC                       <  SRR4302544.1349441/94‑1 (MQ=60)
                                                                      TTTGCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACG                      <  SRR4302544.3116315/94‑1 (MQ=60)
                                                                         GCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCC                   <  SRR4302544.1198456/94‑1 (MQ=60)
                                                                         GCGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCC                   <  SRR4302544.4588491/94‑1 (MQ=60)
                                                                          CGAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCT                  >  SRR4302544.4347887/1‑94 (MQ=60)
                                                                           GAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTC                 >  SRR4302544.4226522/1‑94 (MQ=60)
                                                                           GAGCACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTC                 <  SRR4302544.2633257/94‑1 (MQ=60)
                                                                           GAGCACGTGGGTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTC                 >  SRR4302544.1238451/1‑94 (MQ=60)
                                                                               ACGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACAC             >  SRR4302544.716286/1‑94 (MQ=60)
                                                                                CGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACA            >  SRR4302544.2526016/1‑94 (MQ=60)
                                                                                CGTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACA            >  SRR4302544.348075/1‑94 (MQ=60)
                                                                                 GTGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAA           >  SRR4302544.3675042/1‑94 (MQ=60)
                                                                                  TGGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAAA          >  SRR4302544.2100609/1‑94 (MQ=60)
                                                                                   GGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATA         <  SRR4302544.18575/94‑1 (MQ=60)
                                                                                   GGTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATA         >  SRR4302544.3777647/1‑94 (MQ=60)
                                                                                    GTTTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATAT        >  SRR4302544.2245695/1‑94 (MQ=60)
                                                                                      TTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGA      <  SRR4302544.3333165/94‑1 (MQ=60)
                                                                                      TTGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGA      <  SRR4302544.3024179/94‑1 (MQ=60)
                                                                                       TGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAG     <  SRR4302544.682581/94‑1 (MQ=60)
                                                                                       TGCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAG     <  SRR4302544.682580/94‑1 (MQ=60)
                                                                                        GCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGG    <  SRR4302544.3969558/94‑1 (MQ=60)
                                                                                        GCGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGG    <  SRR4302544.1736119/94‑1 (MQ=60)
                                                                                         CGTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGA   >  SRR4302544.506988/1‑94 (MQ=60)
                                                                                          GTCCCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAC  >  SRR4302544.4418293/1‑94 (MQ=60)
                                                                                            |                                                                                           
TATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGAC  >  NC_000913/3472075‑3472258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑