Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1357	179.3	9500450	96.1%	9129932	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,043,629	A→G	I185T (ATT→ACT)	yihG ←	putative endonuclease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,043,629	0	A	G	99.2%	802.8 / ‑3.5	246	I185T (ATT→ACT)	yihG	putative endonuclease
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (103/141); total (104/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 259 total aligned reads displayed.

CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA  >  NC_000913/4043537‑4043718

  gccgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtg                                                                                         <  2:418388/93‑1 (MQ=255)

   ccgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtgg                                                                                        <  1:323175/93‑1 (MQ=255)

   ccgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtgg                                                                                        <  2:4544264/93‑1 (MQ=255)

    cgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggt                                                                                       <  2:1605029/93‑1 (MQ=255)

    cgccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggt                                                                                       <  2:844137/93‑1 (MQ=255)

     gccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtg                                                                                      <  1:2512615/93‑1 (MQ=255)

     gccTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtg                                                                                      >  1:1476705/1‑93 (MQ=255)

       cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAACTTGACAGtggtggt                                                                                    >  2:2727424/1‑93 (MQ=255)

       cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  1:1333722/93‑1 (MQ=255)

       cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggt                                                                                    <  2:190314/93‑1 (MQ=255)

       cTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggg                                                                                    >  2:3322053/1‑92 (MQ=255)

        tttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtggtg                                                                                   <  2:3765337/93‑1 (MQ=255)

        tttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtggtg                                                                                   >  2:3379545/1‑93 (MQ=255)

        tttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAgtggtggtg                                                                                   >  1:2645937/1‑93 (MQ=255)

        tgtggtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCGGCAACGAAAGAGACAgtggtggtg                                                                                   >  1:4219194/3‑93 (MQ=255)

         ttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGtggtggtgg                                                                                  <  2:2382008/93‑1 (MQ=255)

         ttGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAAtggtggtgg                                                                                  <  1:4007289/93‑1 (MQ=255)

          tggtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTggg                                                                                 <  1:3987298/93‑1 (MQ=255)

          tggtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTggg                                                                                 <  2:3260622/93‑1 (MQ=255)

           ggtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGa                                                                                <  1:1149511/93‑1 (MQ=255)

            gtggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTgggtt                                                                               <  2:4577584/93‑3 (MQ=255)

             tggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATg                                                                              <  2:2394152/93‑1 (MQ=255)

              ggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGc                                                                             <  2:1463376/93‑1 (MQ=255)

              ggCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGc                                                                             <  1:102126/93‑1 (MQ=255)

               ggaacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGAGGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGACGCa                                                                            <  1:1070917/91‑1 (MQ=255)

                caacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAg                                                                           >  1:4102667/1‑93 (MQ=255)

                 aacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAgg                                                                          >  2:1316262/1‑93 (MQ=255)

                 aacaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAgg                                                                          >  2:3825938/1‑93 (MQ=255)

                 aaaaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAgg                                                                          <  2:2057692/90‑1 (MQ=255)

                  acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         >  1:1545256/1‑93 (MQ=255)

                  acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         >  2:1981505/1‑93 (MQ=255)

                  acaaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGc                                                                         <  2:2703288/93‑1 (MQ=255)

                   caaGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCg                                                                        <  1:18427/93‑1 (MQ=255)

                     aGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTGCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGaa                                                                      <  1:411661/93‑1 (MQ=255)

                      gTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGaaa                                                                     >  1:4310208/1‑93 (MQ=255)

                       ttttGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACTAAATTGCCAGTGGTGGTGGGATGCAGGCGaaat                                                                    >  1:463003/1‑92 (MQ=255)

                       ttttGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAAc                                                                    <  1:2871382/93‑1 (MQ=255)

                           gAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACtttt                                                                >  2:3640352/1‑93 (MQ=255)

                              aaGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTcac                                                             >  2:2979667/1‑93 (MQ=255)

                                gtggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACaa                                                           >  2:3694991/1‑93 (MQ=255)

                                 tggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAg                                                          >  1:4681968/1‑93 (MQ=255)

                                  ggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACTGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGa                                                         <  2:1372059/93‑1 (MQ=255)

                                  ggagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGa                                                         >  2:1010161/1‑93 (MQ=255)

                                   gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        <  1:2896126/93‑1 (MQ=255)

                                   gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        <  1:1931649/93‑1 (MQ=255)

                                   gagtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGaa                                                        <  2:3575022/93‑1 (MQ=255)

                                    agtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAc                                                       >  1:806139/1‑93 (MQ=255)

                                    agtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAc                                                       <  2:790638/93‑1 (MQ=255)

                                     gtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACg                                                      <  2:4284955/93‑1 (MQ=255)

                                     gtggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACg                                                      >  1:1285002/1‑93 (MQ=255)

                                      tggGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGc                                                     <  2:2781200/93‑1 (MQ=255)

                                        ggTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCACCTACATTGCCAGTGGTGGTTGAATGCAGGCGAACCTTTTCACAACAACGCCg                                                   >  1:3320481/1‑93 (MQ=255)

                                        ggTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTTGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCg                                                   >  1:3770002/1‑93 (MQ=255)

                                         gTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGa                                                  <  2:1205827/93‑1 (MQ=255)

                                         gTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGa                                                  <  1:1582461/93‑1 (MQ=255)

                                           ttGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGt                                                >  2:3790836/1‑93 (MQ=255)

                                            tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGATTg                                               <  2:2878057/93‑1 (MQ=255)

                                            tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTg                                               >  1:3216220/1‑93 (MQ=255)

                                            tGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTg                                               <  2:580260/93‑1 (MQ=255)

                                             gCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCGGGCGAAACTTTTCACAAGAACGCCGAGTgg                                              >  1:941360/1‑93 (MQ=255)

                                              cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTTGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGt                                             >  1:2081525/1‑93 (MQ=255)

                                              cTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGt                                             <  1:2431628/93‑1 (MQ=255)

                                               tGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGtt                                            <  2:3558002/93‑1 (MQ=255)

                                                gATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGttt                                           >  2:751956/1‑93 (MQ=255)

                                                 aTGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTc                                          >  1:3128286/1‑93 (MQ=255)

                                                  tGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGACGAGGGGGTTCa                                         >  1:2493992/1‑93 (MQ=255)

                                                     ttttCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACa                                      >  2:656054/1‑93 (MQ=255)

                                                       ttCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATc                                    <  1:4651921/93‑1 (MQ=255)

                                                        tCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCt                                   >  1:355241/1‑93 (MQ=255)

                                                         cTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCtt                                  <  1:3407330/93‑1 (MQ=255)

                                                           tGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTa                                <  1:4481066/93‑1 (MQ=255)

                                                              gTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCa                             <  2:274310/93‑1 (MQ=255)

                                                                gAAACGGGAGCCTTCAACGAAATTGACACAGGTGGTGGGATGCAGGCGAAACTTTCCACCAGAACGCCGAGTGGGTTCAACGTCTTTACCACg                           >  2:1550460/1‑93 (MQ=255)

                                                                 aaaCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGt                          <  1:4603004/93‑1 (MQ=255)

                                                                 aaaCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGt                          >  2:1728954/1‑93 (MQ=255)

                                                                   aCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCg                        >  2:1961584/1‑93 (MQ=255)

                                                                      ggAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTc                     >  1:2638116/1‑93 (MQ=255)

                                                                      ggAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTc                     >  2:402935/1‑93 (MQ=255)

                                                                      ggAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTc                     <  1:1722565/93‑1 (MQ=255)

                                                                       gAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTcc                    <  1:4350662/93‑1 (MQ=255)

                                                                       gAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTcc                    <  2:2848273/93‑1 (MQ=255)

                                                                        aGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCg                   >  1:1635827/1‑93 (MQ=255)

                                                                         gCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCgg                  >  1:3601389/1‑93 (MQ=255)

                                                                          ccTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGa                 <  1:4486132/93‑1 (MQ=255)

                                                                           cTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAt                <  1:765327/93‑1 (MQ=255)

                                                                           cTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAt                >  2:222124/1‑93 (MQ=255)

                                                                            ttCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATg               >  2:2646894/1‑93 (MQ=255)

                                                                            ttCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATg               >  1:141389/1‑93 (MQ=255)

                                                                            ttCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGACTCGACATCTTTACCAAGTCGCTCCGGCTg               >  2:504833/1‑93 (MQ=255)

                                                                             tCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGa              >  2:469948/1‑93 (MQ=255)

                                                                              cAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGAc             <  2:2245631/93‑1 (MQ=255)

                                                                               aaCGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACg            <  1:3485338/93‑1 (MQ=255)

                                                                                aCGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGt           >  2:4107211/1‑93 (MQ=255)

                                                                                aCGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGt           >  1:2226244/1‑93 (MQ=255)

                                                                                   aaaTTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGtaat        <  1:2970198/93‑1 (MQ=255)

                                                                                   aaaTTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGtaat        >  1:319827/1‑93 (MQ=255)

                                                                                      ttGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaa     >  1:2308710/1‑93 (MQ=255)

                                                                                        gACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaata   <  2:629269/93‑1 (MQ=255)

                                                                                        gACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTaataaata   <  1:568/93‑1 (MQ=255)

                                                                                         aCAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAataaataa  <  2:2491158/93‑1 (MQ=255)

CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA  >  NC_000913/4043537‑4043718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCG  >  NC_000913/4043536‑4043721

CCTGCCGCCTTTGGTGGCAACAACTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAG                                                                                              <  SRR4302544.3068488/94‑1 (MQ=60)

 CTGCCTCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                             <  SRR4302544.3209273/94‑1 (MQ=60)

 CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                             <  SRR4302544.2200087/94‑1 (MQ=60)

 CTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                             <  SRR4302544.3209271/94‑1 (MQ=60)

 CTGCCGCCTTTGGTGGCAACAAGGTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGT                                                                                             >  SRR4302544.2456380/1‑94 (MQ=60)

  TGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTG                                                                                            >  SRR4302544.3233406/1‑94 (MQ=60)

  TGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTG                                                                                            <  SRR4302544.579980/94‑1 (MQ=60)

   GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGG                                                                                           <  SRR4302544.2174562/94‑1 (MQ=60)

   GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGG                                                                                           <  SRR4302544.264486/94‑1 (MQ=60)

   GCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGG                                                                                           <  SRR4302544.1588457/94‑1 (MQ=60)

    CCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGT                                                                                          <  SRR4302544.326531/94‑1 (MQ=60)

     CGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTG                                                                                         >  SRR4302544.1491312/1‑94 (MQ=60)

      GCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGG                                                                                        >  SRR4302544.1327037/1‑94 (MQ=60)

      GCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGG                                                                                        <  SRR4302544.4550039/94‑1 (MQ=60)

      GCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGG                                                                                        <  SRR4302544.2536733/94‑1 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGT                                                                                       <  SRR4302544.4039736/94‑1 (MQ=60)

       CCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGT                                                                                       <  SRR4302544.4392994/94‑1 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                      <  SRR4302544.1346860/94‑1 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTG                                                                                      >  SRR4302544.2671315/1‑94 (MQ=60)

        CTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGACTTCAACGAAATTGACAGTGGTGGTG                                                                                      >  SRR4302544.3937041/1‑94 (MQ=60)

        CTGTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCGGCAACGAAAGAGACAGTGGTGGTG                                                                                      >  SRR4302544.4261905/1‑94 (MQ=60)

          TTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGG                                                                                    <  SRR4302544.2752796/94‑1 (MQ=60)

          TTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGG                                                                                    <  SRR4302544.4047162/94‑1 (MQ=60)

           TGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGA                                                                                   >  SRR4302544.4677625/1‑94 (MQ=60)

           TGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGA                                                                                   <  SRR4302544.4026992/94‑1 (MQ=60)

           TGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGA                                                                                   >  SRR4302544.4777391/1‑94 (MQ=60)

           TGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGA                                                                                   <  SRR4302544.832579/94‑1 (MQ=60)

            GGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAT                                                                                  <  SRR4302544.1160946/94‑1 (MQ=60)

            GGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAT                                                                                  <  SRR4302544.2987112/94‑1 (MQ=60)

            GGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGAT                                                                                  <  SRR4302544.4708471/94‑1 (MQ=60)

            GGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCATTCAACGAAATTGACAGTGGTGGTGGGAT                                                                                  >  SRR4302544.2666365/1‑94 (MQ=60)

              TGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGC                                                                                >  SRR4302544.3557492/1‑94 (MQ=60)

               GGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCA                                                                               <  SRR4302544.103059/94‑1 (MQ=60)

                GGAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGAGGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGACGCAG                                                                              <  SRR4302544.1081562/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAG                                                                              <  SRR4302544.1116453/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAG                                                                              <  SRR4302544.2088411/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGACGCAG                                                                              <  SRR4302544.1081422/94‑1 (MQ=60)

                GCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAG                                                                              >  SRR4302544.4143479/1‑94 (MQ=60)

                 CAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGG                                                                             >  SRR4302544.742578/1‑94 (MQ=60)

                  AACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGC                                                                            >  SRR4302544.2528917/1‑94 (MQ=60)

                  AACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGC                                                                            >  SRR4302544.1560646/1‑94 (MQ=60)

                    CAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGA                                                                          <  SRR4302544.18613/94‑1 (MQ=60)

                    CAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGGTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGAGGTGGGGGGATGCAGGCGA                                                                          >  SRR4302544.4248311/1‑94 (MQ=60)

                      AGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAA                                                                        >  SRR4302544.4354139/1‑94 (MQ=60)

                      AGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTGCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAA                                                                        <  SRR4302544.415859/94‑1 (MQ=60)

                       GTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACTAAATTGCCAGTGGTGGTGGGATGCAGGCGAAAT                                                                       >  SRR4302544.467550/1‑94 (MQ=60)

                       GTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAAC                                                                       >  SRR4302544.3132120/1‑94 (MQ=60)

                        TTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACT                                                                      <  SRR4302544.2899098/94‑1 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                 <  SRR4302544.1644192/94‑1 (MQ=60)

                             AAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCA                                                                 <  SRR4302544.1644191/94‑1 (MQ=60)

                              AAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCAC                                                                >  SRR4302544.4208056/1‑94 (MQ=60)

                               AAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACA                                                               <  SRR4302544.354251/94‑1 (MQ=60)

                                 GTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAG                                                             >  SRR4302544.4728253/1‑94 (MQ=60)

                                   GGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAA                                                           <  SRR4302544.4711540/94‑1 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCATGCGAAACTTTTCACAAGAAC                                                          <  SRR4302544.4645743/94‑1 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                          <  SRR4302544.2924026/94‑1 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                          <  SRR4302544.3894181/94‑1 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                          >  SRR4302544.813999/1‑94 (MQ=60)

                                    GAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAAC                                                          <  SRR4302544.1950341/94‑1 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCGCAAGAACG                                                         >  SRR4302544.861360/1‑94 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACG                                                         <  SRR4302544.993673/94‑1 (MQ=60)

                                     AGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACG                                                         >  SRR4302544.1297662/1‑94 (MQ=60)

                                        GGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCACCTACATTGCCAGTGGTGGTTGAATGCAGGCGAACCTTTTCACAACAACGCCG                                                      >  SRR4302544.3352113/1‑94 (MQ=53)

                                        GGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTTGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCG                                                      >  SRR4302544.3807381/1‑94 (MQ=60)

                                        GGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCG                                                      >  SRR4302544.3352112/1‑94 (MQ=60)

                                        GGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCG                                                      >  SRR4302544.4282266/1‑94 (MQ=60)

                                          GTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAG                                                    <  SRR4302544.2911699/94‑1 (MQ=60)

                                          GTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAG                                                    <  SRR4302544.3063219/94‑1 (MQ=60)

                                          GTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAG                                                    <  SRR4302544.1598099/94‑1 (MQ=60)

                                          GTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAG                                                    <  SRR4302544.1415923/94‑1 (MQ=60)

                                            TTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTG                                                  >  SRR4302544.3246903/1‑94 (MQ=60)

                                            TTGCTGATGTTTTTCTTGCGTGAAACGGGAGCATTCCACGAATTTGACAGCGGCGGGGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTG                                                  >  SRR4302544.3246904/1‑94 (MQ=60)

                                             TGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCGGGCGAAACTTTTCACAAGAACGCCGAGTGG                                                 >  SRR4302544.950630/1‑94 (MQ=60)

                                             TGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACacagagggg                                                 >  SRR4302544.3612871/1‑85 (MQ=60)

                                             TGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGG                                                 >  SRR4302544.2152478/1‑94 (MQ=60)

                                             TGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGG                                                 >  SRR4302544.4310976/1‑94 (MQ=60)

                                              GCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTTGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGT                                                >  SRR4302544.2101537/1‑94 (MQ=60)

                                              GCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGT                                                >  SRR4302544.186404/1‑94 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGTGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                               <  SRR4302544.2454795/94‑1 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                               <  SRR4302544.1773124/94‑1 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                               >  SRR4302544.3786648/1‑94 (MQ=60)

                                               CTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTT                                               <  SRR4302544.2454794/94‑1 (MQ=60)

                                                TGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTT                                              <  SRR4302544.3426907/94‑1 (MQ=60)

                                                 GATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTC                                             >  SRR4302544.3158011/1‑94 (MQ=60)

                                                 GATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTC                                             >  SRR4302544.2958618/1‑94 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGACGAGGGGGTTCA                                            >  SRR4302544.2517886/1‑94 (MQ=60)

                                                  ATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCAAAACTTTTCACAAGAACGCCGAGTGGTTTCA                                            >  SRR4302544.3185896/1‑94 (MQ=60)

                                                   TGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAA                                           >  SRR4302544.583960/1‑94 (MQ=60)

                                                       TTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATC                                       <  SRR4302544.4668711/94‑1 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                      <  SRR4302544.4697961/94‑1 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                      <  SRR4302544.1318754/94‑1 (MQ=60)

                                                        TTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCT                                      >  SRR4302544.358880/1‑94 (MQ=60)

                                                         TCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTT                                     <  SRR4302544.110986/94‑1 (MQ=60)

                                                          CTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTT                                    <  SRR4302544.2562416/94‑1 (MQ=60)

                                                          CTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTT                                    <  SRR4302544.3439906/94‑1 (MQ=60)

                                                           TTGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTA                                   <  SRR4302544.1843324/94‑1 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAC                                  <  SRR4302544.4526109/94‑1 (MQ=60)

                                                            TGCGTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTAC                                  <  SRR4302544.4526108/94‑1 (MQ=60)

                                                               GTGAAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCAC                               >  SRR4302544.3281064/1‑94 (MQ=60)

                                                                  AACCGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.1486116/94‑1 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACTTC                            >  SRR4302544.3637515/1‑94 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.2511548/94‑1 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.4648757/94‑1 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.4444400/94‑1 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.1714771/94‑1 (MQ=60)

                                                                  AAACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTCCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTC                            <  SRR4302544.1875137/94‑1 (MQ=60)

                                                                   AACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCG                           <  SRR4302544.240070/94‑1 (MQ=60)

                                                                   AACGGGAGCCTTCAACGAAATTGACAGTGGTGGGGGGATGCAGGCGAAAGTTTTCACAAGAACGCCGAGCGGTTTCAACATCTTTACCACctct                           >  SRR4302544.1391386/1‑90 (MQ=60)

                                                                    ACGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGC                          <  SRR4302544.2864856/94‑1 (MQ=60)

                                                                     CGGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCACCATCTTTACCACGTCGCT                         <  SRR4302544.4098878/94‑1 (MQ=60)

                                                                      GGGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTC                        >  SRR4302544.2663402/1‑94 (MQ=60)

                                                                       GGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCC                       <  SRR4302544.3427654/94‑1 (MQ=60)

                                                                       GGAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCC                       <  SRR4302544.1739478/94‑1 (MQ=60)

                                                                        GAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCG                      >  SRR4302544.4149597/1‑94 (MQ=60)

                                                                        GAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCG                      <  SRR4302544.4394866/94‑1 (MQ=60)

                                                                        GAGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCG                      >  SRR4302544.1651926/1‑94 (MQ=60)

                                                                         AGCCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGG                     >  SRR4302544.3636213/1‑94 (MQ=60)

                                                                           CCTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGAT                   <  SRR4302544.4531199/94‑1 (MQ=60)

                                                                            CTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATG                  <  SRR4302544.772783/94‑1 (MQ=60)

                                                                            CTTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATG                  >  SRR4302544.142725/1‑94 (MQ=60)

                                                                             TTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGA                 <  SRR4302544.153930/94‑1 (MQ=60)

                                                                             TTCAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGA                 <  SRR4302544.153929/94‑1 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG               <  SRR4302544.3237530/94‑1 (MQ=60)

                                                                               CAACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACG               <  SRR4302544.1478899/94‑1 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT              <  SRR4302544.2382866/94‑1 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT              >  SRR4302544.81200/1‑94 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT              >  SRR4302544.2247188/1‑94 (MQ=60)

                                                                                AACGAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGT              <  SRR4302544.3518554/94‑1 (MQ=60)

                                                                                AACGAAATTGACAGTGGTAGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGA              >  SRR4302544.407823/1‑94 (MQ=60)

                                                                                   GAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAAT           >  SRR4302544.323150/1‑94 (MQ=60)

                                                                                   GAAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAAT           >  SRR4302544.1168549/1‑94 (MQ=60)

                                                                                    AAATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATA          <  SRR4302544.2998677/94‑1 (MQ=60)

                                                                                      ATTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAA        >  SRR4302544.2330533/1‑94 (MQ=60)

                                                                                       TTGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAAT       <  SRR4302544.3508161/94‑1 (MQ=60)

                                                                                        TGACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATA      <  SRR4302544.4691146/94‑1 (MQ=60)

                                                                                         GACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA     <  SRR4302544.576/94‑1 (MQ=60)

                                                                                         GACAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAA     <  SRR4302544.4436174/94‑1 (MQ=60)

                                                                                          ACAGTGGTGGGGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAG    <  SRR4302544.2272513/94‑1 (MQ=60)

                                                                                           CAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGC   <  SRR4302544.3190255/94‑1 (MQ=60)

                                                                                           CAGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGC   <  SRR4302544.2095154/94‑1 (MQ=60)

                                                                                            AGTGGTGGTGGGATGCAGGCGAAACTTTTCCCAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCG  <  SRR4302544.124009/94‑1 (MQ=60)

                                                                                            AGTGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCG  >  SRR4302544.2989078/1‑94 (MQ=60)

CCTGCCGCCTTTGGTGGCAACAAGTTTTGAAAAGTGGAGTGGGTTTGCTGATGTTTTTCTTGCGTGAAACGGGAGCCTTCAACGAAATTGACAATGGTGGTGGGATGCAGGCGAAACTTTTCACAAGAACGCCGAGTGGTTTCAACATCTTTACCACGTCGCTCCGGATGACGTAATAAATAAGCG  >  NC_000913/4043536‑4043721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`