Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,113,690 C→T G371G (GGG→GGA)  glpK ← glycerol kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,113,6900CT96.5% 492.2 / 1.1 144G371G (GGG→GGA) glpKglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base T (74/65);  total (78/66)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 148 total aligned reads displayed.

ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT  >  NC_000913/4113600‑4113781
                                                                                          |                                                                                           
 ccAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCa                                                                                          >  2:1713756/1‑93 (MQ=255)
 ccAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCa                                                                                          >  2:4144899/1‑93 (MQ=255)
   agagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACg                                                                                        >  2:4383630/1‑93 (MQ=255)
    gcgTCGGCCTCCCGCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGa                                                                                       <  2:227426/91‑1 (MQ=255)
      gTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGt                                                                                     >  1:900865/1‑93 (MQ=255)
        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCa                                                                                   <  2:1262721/93‑1 (MQ=255)
        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCa                                                                                   <  2:1325172/93‑1 (MQ=255)
         ggCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAg                                                                                  <  1:57585/93‑1 (MQ=255)
          gCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGa                                                                                 <  1:1345767/93‑1 (MQ=255)
          gCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGa                                                                                 <  2:4596469/93‑1 (MQ=255)
           ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAc                                                                                <  2:3934480/93‑1 (MQ=255)
           ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAc                                                                                <  1:3322651/93‑1 (MQ=255)
           ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAc                                                                                >  1:2884085/1‑93 (MQ=255)
            cTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAcc                                                                               <  2:53071/93‑1 (MQ=255)
             tGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCg                                                                              >  1:2061137/1‑93 (MQ=255)
              gCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGa                                                                             >  2:2676557/1‑93 (MQ=255)
                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaa                                                                           >  2:2549123/1‑93 (MQ=255)
                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaa                                                                           <  1:2306494/93‑1 (MQ=255)
                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaa                                                                           <  1:2720924/93‑1 (MQ=255)
                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaa                                                                           <  2:4100284/93‑1 (MQ=255)
                 tCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaaa                                                                          <  2:4712754/93‑1 (MQ=255)
                  cGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAAt                                                                         <  1:2027430/93‑1 (MQ=255)
                   gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATc                                                                        <  2:2684753/93‑1 (MQ=255)
                     ttCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGc                                                                      >  2:4158346/1‑93 (MQ=255)
                          gCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgc                                                                 <  1:911736/93‑1 (MQ=255)
                          gCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgc                                                                 >  1:3369444/1‑93 (MQ=255)
                            ccGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgcgc                                                               <  2:1596957/92‑1 (MQ=255)
                            aCGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgcgc                                                               >  2:1912659/1‑93 (MQ=255)
                             cGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCcgcgcg                                                              >  1:1563131/1‑93 (MQ=255)
                              gacACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGCCAGACCGAAACTCGCCCCgcgcgc                                                             >  2:1699033/3‑93 (MQ=255)
                              gTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCgcgcgc                                                             >  1:22953/1‑93 (MQ=255)
                              gTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGAGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCgcgcgc                                                             >  1:1066154/1‑93 (MQ=255)
                               tCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCa                                                            >  1:1721213/1‑93 (MQ=255)
                               tCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCa                                                            >  1:2689114/1‑93 (MQ=255)
                                cACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATAGCCCCGCGCGCAt                                                           >  2:4312653/1‑93 (MQ=255)
                                 aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                          >  2:924734/1‑93 (MQ=255)
                                 aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                          <  2:3500147/93‑1 (MQ=255)
                                 aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                          >  1:2997749/1‑93 (MQ=255)
                                  cgcgTCTGCTAAGCAATAAACTCCAGCGTCGCACGTTTCATGTGGTTAGCGTTCACTCCACAAGTCGGACCGAAAATCACCACGCGCGCATAc                                                         <  1:4209832/93‑1 (MQ=255)
                                   gcgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                        <  1:1790726/93‑1 (MQ=255)
                                    cgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACgg                                                       >  1:2488469/1‑93 (MQ=255)
                                     gTCTGATAAGCAATAGCCGCCAGCGTCGCGCGTATAATTTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACggg                                                      <  1:3987335/93‑1 (MQ=255)
                                     gTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACggg                                                      >  1:4422748/1‑93 (MQ=255)
                                      tCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGt                                                     >  2:2808115/1‑93 (MQ=255)
                                       cTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTc                                                    <  2:2842559/93‑1 (MQ=255)
                                        tGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTcc                                                   >  2:2768752/1‑93 (MQ=255)
                                        tGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTcc                                                   >  2:4522035/1‑93 (MQ=255)
                                         gATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTccc                                                  <  2:3035046/93‑1 (MQ=255)
                                         gATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTccc                                                  >  2:1077302/1‑93 (MQ=255)
                                         gATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTccc                                                  <  1:776457/93‑1 (MQ=255)
                                          aTAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                                 >  1:3741882/1‑93 (MQ=255)
                                          aTAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTAAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                                 <  2:4127749/93‑1 (MQ=255)
                                            aaGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGt                                               >  1:3789193/1‑93 (MQ=255)
                                            aaGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGt                                               >  2:1646991/1‑93 (MQ=255)
                                             aGCAATAGACTCCAGGGCGCCCCGAATAATGTGGTTAGCGTTAACTCCCGGGGTCGGCCCGAAATTCGCCCCGCCCGCATACGGGCCCCAGtc                                              >  2:3758711/1‑92 (MQ=255)
                                             aGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGtc                                              >  1:3376209/1‑92 (MQ=255)
                                              gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                             >  1:72909/1‑93 (MQ=255)
                                              gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                             >  1:4337401/1‑93 (MQ=255)
                                              gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                             <  2:3170595/93‑1 (MQ=255)
                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                           >  1:2036979/1‑93 (MQ=255)
                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                           <  2:709897/93‑1 (MQ=255)
                                                 aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                          >  1:3020985/1‑93 (MQ=255)
                                                  tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgcg                                         <  2:1878423/93‑1 (MQ=255)
                                                  tAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgcg                                         <  1:4642160/93‑1 (MQ=255)
                                                        ccAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa                                   >  1:3111052/1‑93 (MQ=255)
                                                        ccAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa                                   <  1:3861723/93‑1 (MQ=255)
                                                         cAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAg                                  >  1:1283062/1‑93 (MQ=255)
                                                          aGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGc                                 <  2:2348723/93‑1 (MQ=255)
                                                          aGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGc                                 >  1:3173824/1‑93 (MQ=255)
                                                           gCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGcc                                <  2:2289615/93‑1 (MQ=255)
                                                           gCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGcc                                >  2:850046/1‑93 (MQ=255)
                                                              tCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCgg                             <  1:3435695/93‑1 (MQ=255)
                                                                 cgcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaa                          >  1:4708272/1‑93 (MQ=255)
                                                                  gcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                         <  1:4105696/93‑1 (MQ=255)
                                                                  gcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                         <  2:3136027/93‑1 (MQ=255)
                                                                  gcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                         >  2:1537400/1‑93 (MQ=255)
                                                                  gcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                         >  2:3579682/1‑93 (MQ=255)
                                                                   cgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAt                        <  2:3047648/93‑1 (MQ=255)
                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                      >  2:1100472/1‑93 (MQ=255)
                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                      >  1:2412712/1‑93 (MQ=255)
                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                      >  2:3121021/1‑93 (MQ=255)
                                                                      ataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGcc                     <  2:1787833/93‑1 (MQ=255)
                                                                      ataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGcc                     >  1:1946271/1‑93 (MQ=255)
                                                                       taATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                    <  2:4714044/93‑1 (MQ=255)
                                                                       taATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                    <  2:3588642/93‑1 (MQ=255)
                                                                       taATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                    >  1:1733745/1‑93 (MQ=255)
                                                                       taATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                    <  1:4501707/93‑1 (MQ=255)
                                                                          tgtgGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGaa                 >  1:4723227/1‑93 (MQ=255)
                                                                            tgGTTAGCGTTCACCCCACGAGGCAGACCGAAAATCGCCCCGCGCGCATACGGGTCACAGTACGGCGCACCGAGCCCGGTAAGTGCGGGAccc               >  1:1587643/1‑93 (MQ=255)
                                                                              gTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACcac             >  1:2904597/1‑93 (MQ=255)
                                                                              gTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGGCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACcac             >  2:514920/1‑93 (MQ=255)
                                                                                tAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACAt           <  1:1816058/93‑1 (MQ=255)
                                                                                tAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACAt           <  2:1899348/93‑1 (MQ=255)
                                                                                  gCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATac         <  1:598888/93‑1 (MQ=255)
                                                                                   cGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATaca        <  2:2843051/93‑1 (MQ=255)
                                                                                    gTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacac       >  1:1799362/1‑93 (MQ=255)
                                                                                     ttCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacaca      >  2:3942619/1‑93 (MQ=255)
                                                                                     ttCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacaca      >  2:744775/1‑93 (MQ=255)
                                                                                       cACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACAcc    <  1:4617556/93‑1 (MQ=255)
                                                                                         ccccACGAGTAAGACCGAAAAGCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCCCCCAGCCCGGTAAATGCCGGAACCACATACACACCAt  <  1:2166661/93‑1 (MQ=255)
                                                                                          |                                                                                           
ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT  >  NC_000913/4113600‑4113781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATT  >  NC_000913/4113597‑4113782
                                                                                             |                                                                                            
GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACT                                                                                              <  SRR4302544.534185/94‑1 (MQ=60)
GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACT                                                                                              <  SRR4302544.3456293/94‑1 (MQ=60)
 ATACCAGAGTCGTCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTC                                                                                             >  SRR4302544.1959547/1‑94 (MQ=60)
   ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCA                                                                                           <  SRR4302544.1454994/94‑1 (MQ=60)
    CCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCAC                                                                                          <  SRR4302544.1992697/94‑1 (MQ=60)
        AGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGT                                                                                      >  SRR4302544.878667/1‑94 (MQ=60)
        AGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGT                                                                                      >  SRR4302544.909780/1‑94 (MQ=60)
            GGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGA                                                                                  <  SRR4302544.58164/94‑1 (MQ=60)
             GCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAC                                                                                 >  SRR4302544.2911901/1‑94 (MQ=60)
             GCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAC                                                                                 <  SRR4302544.1359088/94‑1 (MQ=60)
              CCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACC                                                                                <  SRR4302544.3354302/94‑1 (MQ=60)
               CTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCG                                                                               >  SRR4302544.308314/1‑94 (MQ=60)
               CTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCG                                                                               >  SRR4302544.2077384/1‑94 (MQ=60)
               CTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCG                                                                               >  SRR4302544.2080959/1‑94 (MQ=60)
                  CATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAA                                                                            >  SRR4302544.3798620/1‑94 (MQ=60)
                   ATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAA                                                                           <  SRR4302544.2747123/94‑1 (MQ=60)
                   ATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAA                                                                           <  SRR4302544.2328289/94‑1 (MQ=60)
                    TCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAAT                                                                          <  SRR4302544.554265/94‑1 (MQ=60)
                     CGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATC                                                                         <  SRR4302544.2047078/94‑1 (MQ=60)
                          CCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCAAAAATCGCCCC                                                                    >  SRR4302544.979162/1‑94 (MQ=60)
                            AGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGC                                                                  >  SRR4302544.3401606/1‑94 (MQ=60)
                             GCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCG                                                                 <  SRR4302544.920752/94‑1 (MQ=60)
                              CACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGC                                                                >  SRR4302544.2198505/1‑94 (MQ=60)
                              CACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGC                                                                <  SRR4302544.1674292/94‑1 (MQ=60)
                               ACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCG                                                               >  SRR4302544.1578640/1‑94 (MQ=60)
                                CGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGC                                                              >  SRR4302544.23176/1‑94 (MQ=60)
                                CGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGAGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGC                                                              >  SRR4302544.1076753/1‑94 (MQ=60)
                                 GTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCA                                                             >  SRR4302544.2714902/1‑94 (MQ=60)
                                 GTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCA                                                             >  SRR4302544.1738119/1‑94 (MQ=60)
                                   CACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATA                                                           >  SRR4302544.3026493/1‑94 (MQ=60)
                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                        <  SRR4302544.2987520/94‑1 (MQ=60)
                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                        >  SRR4302544.2512304/1‑94 (MQ=60)
                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                        <  SRR4302544.1752688/94‑1 (MQ=60)
                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                        <  SRR4302544.1808230/94‑1 (MQ=60)
                                       CGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGG                                                       <  SRR4302544.4408968/94‑1 (MQ=60)
                                       CGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGG                                                       >  SRR4302544.4467368/1‑94 (MQ=60)
                                        GTCTGATAAGCAATAGCCGCCAGCGTCGCGCGTATAATTTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGT                                                      <  SRR4302544.4027029/94‑1 (MQ=60)
                                            GATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCA                                                  <  SRR4302544.783993/94‑1 (MQ=60)
                                            GATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCA                                                  >  SRR4302544.3779061/1‑94 (MQ=60)
                                              TAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGT                                                >  SRR4302544.3826711/1‑94 (MQ=60)
                                               AAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTC                                               >  SRR4302544.3408438/1‑94 (MQ=60)
                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAC                                              >  SRR4302544.73612/1‑94 (MQ=60)
                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAC                                              <  SRR4302544.3395073/94‑1 (MQ=60)
                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAC                                              >  SRR4302544.4381542/1‑94 (MQ=60)
                                                 GCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACG                                             <  SRR4302544.2780663/94‑1 (MQ=60)
                                                  CAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGG                                            >  SRR4302544.2056660/1‑94 (MQ=60)
                                                  CAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGG                                            >  SRR4302544.4016609/1‑94 (MQ=60)
                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                           >  SRR4302544.3049938/1‑94 (MQ=60)
                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                           >  SRR4302544.4696438/1‑94 (MQ=60)
                                                     TAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGC                                         <  SRR4302544.4688136/94‑1 (MQ=60)
                                                       GACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAC                                       >  SRR4302544.4190968/1‑94 (MQ=60)
                                                        ACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATNCGGGTCCCAGTACGGCGCACC                                      >  SRR4302544.3432527/1‑94 (MQ=60)
                                                          TCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCA                                    >  SRR4302544.3140652/1‑94 (MQ=60)
                                                           CCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAG                                   >  SRR4302544.1295701/1‑94 (MQ=60)
                                                           CCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAG                                   <  SRR4302544.3900064/94‑1 (MQ=60)
                                                            CAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGC                                  >  SRR4302544.3204027/1‑94 (MQ=60)
                                                                 TCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGT                             <  SRR4302544.3468542/94‑1 (MQ=60)
                                                                  CGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTA                            >  SRR4302544.2386207/1‑94 (MQ=60)
                                                                   GCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAA                           >  SRR4302544.4754790/1‑94 (MQ=60)
                                                                     GCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAT                         <  SRR4302544.4146525/94‑1 (MQ=60)
                                                                      CGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                        <  SRR4302544.2599220/94‑1 (MQ=60)
                                                                       GTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGC                       >  SRR4302544.2435658/1‑94 (MQ=60)
                                                                        TATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCC                      >  SRR4302544.1965117/1‑94 (MQ=60)
                                                                         ATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCG                     >  SRR4302544.1750739/1‑94 (MQ=60)
                                                                         ATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCG                     >  SRR4302544.675674/1‑94 (MQ=60)
                                                                          TAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGG                    <  SRR4302544.4546876/94‑1 (MQ=60)
                                                                            ATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAA                  >  SRR4302544.4769862/1‑94 (MQ=60)
                                                                              GTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACC                <  SRR4302544.1833234/94‑1 (MQ=60)
                                                                              GTGGTTAGCGTTCACCCCACGAGGCAGACCGAAAATCGCCCCGCGCGCATACGGGTCACAGTACGGCGCACCGAGCCCGGTAAGTGCGGGACCC                >  SRR4302544.1603313/1‑94 (MQ=60)
                                                                                GGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCAC              >  SRR4302544.2932564/1‑94 (MQ=60)
                                                                                   TAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATA           >  SRR4302544.4565661/1‑94 (MQ=60)
                                                                                   TAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATA           <  SRR4302544.1833782/94‑1 (MQ=60)
                                                                                     GCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACA         <  SRR4302544.604757/94‑1 (MQ=60)
                                                                                      CGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACAC        >  SRR4302544.1816950/1‑94 (MQ=60)
                                                                                          CACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCA    <  SRR4302544.4663390/94‑1 (MQ=60)
                                                                                           ACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT   >  SRR4302544.45065/1‑94 (MQ=60)
                                                                                            CTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATT  <  SRR4302544.3340307/94‑1 (MQ=60)
                                                                                            CCCCACGAGTAAGACCGAAAAGCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCCCCCAGCCCGGTAAATGCCGGAACCACATACACACCATT  <  SRR4302544.2187223/94‑1 (MQ=60)
                                                                                             |                                                                                            
GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATT  >  NC_000913/4113597‑4113782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑