Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,113,690	C→T	G371G (GGG→GGA)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,113,690	0	C	T	100.0%	815.8 / NA	217	G371G (GGG→GGA)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (106/111); total (106/111)

Only 100 of 224 total aligned reads displayed.

ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACC  >  NC_000913/4113600‑4113779

 ccAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAAGGTGGTTAGCGTTCACTCCa                                                                                        <  1:6518743/93‑1 (MQ=255)

  cAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCAc                                                                                       <  2:2855379/93‑1 (MQ=255)

    gagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGa                                                                                     <  2:5545376/93‑1 (MQ=255)

    gagTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGa                                                                                     >  1:2206287/1‑93 (MQ=255)

        cGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCa                                                                                 <  2:2145021/93‑1 (MQ=255)

          gCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGa                                                                               >  2:6427100/1‑93 (MQ=255)

           ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAc                                                                              <  2:4380235/93‑1 (MQ=255)

           ccTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAc                                                                              <  1:673009/93‑1 (MQ=255)

            cTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAcc                                                                             >  2:398792/1‑93 (MQ=255)

            cTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAcc                                                                             <  1:6211649/93‑1 (MQ=255)

            cTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGAcc                                                                             >  2:3285431/1‑93 (MQ=255)

             tGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCg                                                                            <  2:1439455/93‑1 (MQ=255)

             tGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCg                                                                            >  2:1171379/1‑93 (MQ=255)

              gCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGa                                                                           <  1:3694814/93‑1 (MQ=255)

                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaa                                                                         >  2:3181408/1‑93 (MQ=255)

                aTCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACAGaaa                                                                         >  2:2775891/1‑93 (MQ=255)

                 tCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGaaaa                                                                        >  2:2204400/1‑93 (MQ=255)

                   gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATc                                                                      <  2:347457/93‑1 (MQ=255)

                   gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATc                                                                      <  1:5622789/93‑1 (MQ=255)

                   gCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATc                                                                      <  1:1220924/93‑1 (MQ=255)

                    cTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCg                                                                     >  1:4573463/1‑93 (MQ=255)

                     ttCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGc                                                                    >  2:6120109/1‑93 (MQ=255)

                     ttCCAGCACGTCACGCGTCTGATAAGCAATAGACTACAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGc                                                                    >  1:3362982/1‑93 (MQ=255)

                      tCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAACATCGcc                                                                   >  1:1925558/1‑93 (MQ=255)

                      tCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGcc                                                                   <  2:6556401/93‑1 (MQ=255)

                       ccAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGccc                                                                  >  2:3863058/1‑93 (MQ=255)

                        cAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGcccc                                                                 <  1:1673427/93‑1 (MQ=255)

                        cAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGcccc                                                                 <  2:373955/93‑1 (MQ=255)

                         aGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcg                                                                >  2:4057385/1‑93 (MQ=255)

                          gCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgc                                                               >  1:3926890/1‑93 (MQ=255)

                          gCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgc                                                               <  1:2634559/93‑1 (MQ=255)

                          gCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGGCAGACCGAAAATCGCCCcgc                                                               >  1:2062958/1‑93 (MQ=255)

                            aCGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgcgc                                                             >  2:3142094/1‑93 (MQ=255)

                            aCGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgcgc                                                             >  2:4180824/1‑93 (MQ=255)

                            aCGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCcgcgc                                                             >  1:4034450/1‑93 (MQ=255)

                               tCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCa                                                          <  2:3005659/93‑1 (MQ=255)

                                 aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                        >  2:2026778/1‑93 (MQ=255)

                                 aCGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATa                                                        <  2:2620969/93‑1 (MQ=255)

                                   gcgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                      >  2:5327581/1‑93 (MQ=255)

                                   gcgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACg                                                      <  2:704077/93‑1 (MQ=255)

                                    cgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACgg                                                     >  1:779743/1‑93 (MQ=255)

                                    cgTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACgg                                                     <  2:1546926/93‑1 (MQ=255)

                                       cTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCGGCGCGCATACGGGTc                                                  >  1:1331813/1‑93 (MQ=255)

                                       cTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTc                                                  <  1:6636012/93‑1 (MQ=255)

                                        tGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTcc                                                 <  2:2002073/93‑1 (MQ=255)

                                         gATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTccc                                                >  2:3244988/1‑93 (MQ=255)

                                          aTAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                               <  1:274186/93‑1 (MQ=255)

                                          aTAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCa                                               <  1:4879706/93‑1 (MQ=255)

                                            aaGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGt                                             >  1:5832158/1‑93 (MQ=255)

                                            aaGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGt                                             >  1:5598118/1‑93 (MQ=255)

                                              gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                           <  2:6378157/93‑1 (MQ=255)

                                              gCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAc                                           >  1:1039645/1‑93 (MQ=255)

                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                         >  1:2919264/1‑93 (MQ=255)

                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                         <  1:1130335/93‑1 (MQ=255)

                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACgg                                         <  1:2849118/93‑1 (MQ=255)

                                                aaTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAAgg                                         >  1:1745798/1‑93 (MQ=255)

                                                 aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                        <  2:6154131/93‑1 (MQ=255)

                                                 aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                        >  1:1980096/1‑93 (MQ=255)

                                                 aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                        <  1:5998670/93‑1 (MQ=255)

                                                 aTAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgc                                        >  1:4731507/1‑93 (MQ=255)

                                                   aGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGgcgc                                      >  2:3641421/1‑93 (MQ=255)

                                                     aCTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAc                                    >  2:354314/1‑93 (MQ=255)

                                                        ccAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa                                 >  1:6321568/1‑93 (MQ=255)

                                                        ccAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCa                                 >  2:869187/1‑93 (MQ=255)

                                                         cAGCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAg                                <  2:1001730/93‑1 (MQ=255)

                                                           gCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGcc                              <  2:2456240/93‑1 (MQ=255)

                                                           gCGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGcc                              <  2:6065216/93‑1 (MQ=255)

                                                            cGTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGccc                             >  2:5617737/1‑93 (MQ=255)

                                                             gTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCg                            >  1:2416016/1‑93 (MQ=255)

                                                             gTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCg                            >  1:905023/1‑93 (MQ=255)

                                                             gTCGCGCGTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCg                            >  1:4377155/1‑93 (MQ=255)

                                                                gcgcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTa                         <  1:1110603/93‑1 (MQ=255)

                                                                gcgcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTa                         >  1:1799878/1‑93 (MQ=255)

                                                                 cgcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaa                        >  1:4696834/1‑93 (MQ=255)

                                                                  gcgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                       <  2:2336512/93‑1 (MQ=255)

                                                                  gcgTATAATGTGGTTAGCGTGCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTaaa                       <  1:3900680/93‑1 (MQ=255)

                                                                   cgTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAt                      <  1:3401420/93‑1 (MQ=255)

                                                                    gTATAATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATg                     >  1:1785087/1‑93 (MQ=255)

                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                    >  1:3589884/1‑93 (MQ=255)

                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                    <  2:6381782/93‑1 (MQ=255)

                                                                     tataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGc                    >  2:5010176/1‑93 (MQ=255)

                                                                      ataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGcc                   >  2:3870079/1‑93 (MQ=255)

                                                                      ataATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGcc                   <  1:6399074/93‑1 (MQ=255)

                                                                       taATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                  >  2:5794864/1‑93 (MQ=255)

                                                                       taATGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCg                  <  2:162475/93‑1 (MQ=255)

                                                                         aTGTGGTTAGCGTTCGCTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGa                >  1:1579936/1‑93 (MQ=255)

                                                                         aTGTGGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGa                <  1:3142824/93‑1 (MQ=255)

                                                                          tgtgGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGaa               >  1:4766682/1‑93 (MQ=255)

                                                                           gtgGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc              <  1:3471187/93‑1 (MQ=255)

                                                                           gtgGTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAc              >  1:5035930/1‑93 (MQ=255)

                                                                             ggTTAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACca            <  2:6435982/93‑1 (MQ=255)

                                                                              gTTAGCGTTCTCTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACcac           <  2:4416177/93‑1 (MQ=255)

                                                                               ttAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACcaca          <  2:5948593/93‑1 (MQ=255)

                                                                                tAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACAt         <  1:2197519/93‑1 (MQ=255)

                                                                                tAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACAt         <  1:3951737/93‑1 (MQ=255)

                                                                                tAGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACAt         >  2:6570349/1‑93 (MQ=255)

                                                                                 aGCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATa        >  2:5146320/1‑93 (MQ=255)

                                                                                  gCGTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATac       >  1:492477/1‑93 (MQ=255)

                                                                                    gTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacac     >  2:1247457/1‑93 (MQ=255)

                                                                                    gTTCACTCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATacac     >  2:188859/1‑93 (MQ=255)

ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCACCCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACC  >  NC_000913/4113600‑4113779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑CCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTG  >  NC_000913/4113597‑4113783

GATACCAGAGTCGGCCTGCAGCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑T                                                                                               <  SRR4302536.656634/94‑1 (MQ=60)

 ATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAACGTGGTTAGCGTTCAC‑TC                                                                                              >  SRR4302536.3578283/1‑94 (MQ=60)

 ATACCAGAGACGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATCAGCAATAGACTCCACCGGCGCGCGGATAATGTGGTTAGAGTTCAC‑TC                                                                                              >  SRR4302536.3463870/1‑94 (MQ=60)

   ACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGAGTATAATGTGGTTAGCGTTCACTCCC                                                                                             >  SRR4302536.1694658/1‑94 (MQ=60)

    CCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCAC                                                                                           <  SRR4302536.635080/94‑1 (MQ=60)

    CCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAAGGTGGTTAGCGTTCAC‑TCCAC                                                                                           <  SRR4302536.6712942/94‑1 (MQ=60)

     CAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACG                                                                                          <  SRR4302536.5323058/94‑1 (MQ=60)

      AGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGA                                                                                         >  SRR4302536.2272906/1‑94 (MQ=60)

        AGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGT                                                                                       <  SRR4302536.1210872/94‑1 (MQ=60)

        AGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGT                                                                                       <  SRR4302536.2400203/94‑1 (MQ=60)

          GCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCA                                                                                     <  SRR4302536.3499011/94‑1 (MQ=60)

           CGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAG                                                                                    >  SRR4302536.4884411/1‑94 (MQ=60)

             GCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGAC                                                                                  <  SRR4302536.1276911/94‑1 (MQ=60)

              CCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACC                                                                                 <  SRR4302536.692707/94‑1 (MQ=60)

              CCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACC                                                                                 <  SRR4302536.481787/94‑1 (MQ=60)

               CTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCG                                                                                <  SRR4302536.6396716/94‑1 (MQ=60)

                 GCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAA                                                                              <  SRR4302536.3805311/94‑1 (MQ=60)

                   ATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAA                                                                            >  SRR4302536.5041621/1‑94 (MQ=60)

                    TCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAAT                                                                           <  SRR4302536.1101123/94‑1 (MQ=60)

                      GCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCG                                                                         <  SRR4302536.2752770/94‑1 (MQ=60)

                      GCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCG                                                                         <  SRR4302536.5789810/94‑1 (MQ=60)

                      GCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCG                                                                         <  SRR4302536.1257595/94‑1 (MQ=60)

                      GCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCG                                                                         >  SRR4302536.4710726/1‑94 (MQ=60)

                      GCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCG                                                                         <  SRR4302536.2944765/94‑1 (MQ=60)

                       CTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGC                                                                        >  SRR4302536.3796216/1‑94 (MQ=60)

                       CTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTACAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGC                                                                        >  SRR4302536.3462855/1‑94 (MQ=60)

                        TTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAACATCGCC                                                                       >  SRR4302536.1983493/1‑94 (MQ=60)

                         TCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCC                                                                      <  SRR4302536.4379843/94‑1 (MQ=60)

                           CAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCG                                                                    <  SRR4302536.4876888/94‑1 (MQ=60)

                           CAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCG                                                                    >  SRR4302536.2266387/1‑94 (MQ=60)

                           CAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCG                                                                    <  SRR4302536.1721957/94‑1 (MQ=60)

                            AGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGC                                                                   <  SRR4302536.3514102/94‑1 (MQ=60)

                            AGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGC                                                                   >  SRR4302536.4043574/1‑94 (MQ=60)

                            AGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGGCAGACCGAAAATCGCCCCGC                                                                   >  SRR4302536.2125090/1‑94 (MQ=60)

                             GCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCG                                                                  <  SRR4302536.2713570/94‑1 (MQ=60)

                             GCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCG                                                                  <  SRR4302536.2750524/94‑1 (MQ=60)

                              CACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGC                                                                 >  SRR4302536.4154094/1‑94 (MQ=60)

                              CACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGC                                                                 <  SRR4302536.3417857/94‑1 (MQ=60)

                              CACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGC                                                                 >  SRR4302536.6794138/1‑94 (MQ=60)

                               ACGTCACGCTTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCCC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCG                                                                <  SRR4302536.2648531/94‑1 (MQ=60)

                               ACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCG                                                                <  SRR4302536.1850465/94‑1 (MQ=60)

                                CGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGAGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGA                                                               >  SRR4302536.6516379/1‑94 (MQ=60)

                                  TCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCAT                                                             <  SRR4302536.1466272/94‑1 (MQ=60)

                                   CACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATA                                                            >  SRR4302536.3268059/1‑94 (MQ=60)

                                     CGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACG                                                          <  SRR4302536.3933445/94‑1 (MQ=60)

                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                         >  SRR4302536.6024616/1‑94 (MQ=60)

                                      GCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGG                                                         >  SRR4302536.802589/1‑94 (MQ=60)

                                       CGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGG                                                        >  SRR4302536.1725421/1‑94 (MQ=60)

                                       CGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTCAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGG                                                        <  SRR4302536.3677300/94‑1 (MQ=60)

                                         TCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCGGCGCGCATACGGGTC                                                      >  SRR4302536.1371568/1‑94 (MQ=60)

                                          CTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCC                                                     <  SRR4302536.6833369/94‑1 (MQ=60)

                                          CTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCC                                                     >  SRR4302536.4120416/1‑94 (MQ=60)

                                          CTGATAAGCAATAGACTCCAGCGTCGCGCGCATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGCCCGAAAATCGCCCCGCGCGCATACGGGTCC                                                     >  SRR4302536.2158750/1‑94 (MQ=60)

                                             ATAAGCAATAGCCTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCCC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAG                                                  <  SRR4302536.4059237/94‑1 (MQ=60)

                                             ATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAG                                                  <  SRR4302536.5026519/94‑1 (MQ=60)

                                             ATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAG                                                  <  SRR4302536.281478/94‑1 (MQ=60)

                                              TAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGT                                                 >  SRR4302536.5764528/1‑94 (MQ=60)

                                              TAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGT                                                 >  SRR4302536.6005925/1‑94 (MQ=60)

                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTTC                                               <  SRR4302536.6626725/94‑1 (MQ=60)

                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAC                                               <  SRR4302536.5827003/94‑1 (MQ=60)

                                                AGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAC                                               >  SRR4302536.1070518/1‑94 (MQ=60)

                                                  CAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGG                                             >  SRR4302536.3005424/1‑94 (MQ=60)

                                                  CAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTAAGG                                             >  SRR4302536.1796522/1‑94 (MQ=60)

                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                            <  SRR4302536.2933365/94‑1 (MQ=60)

                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                            >  SRR4302536.4873884/1‑94 (MQ=60)

                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                            >  SRR4302536.2039642/1‑94 (MQ=60)

                                                   AATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGC                                            <  SRR4302536.1164305/94‑1 (MQ=60)

                                                    ATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCG                                           <  SRR4302536.6177491/94‑1 (MQ=60)

                                                      AGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCA                                         >  SRR4302536.856957/1‑94 (MQ=60)

                                                      AGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCA                                         >  SRR4302536.984923/1‑94 (MQ=60)

                                                      AGACTCCAGCGTCGCGCGGATAATGTGGTTAGCGTTCAC‑TCCACGAGTCCTACCGAAAATCGCCCCGCGCGCATACGtgtctgtgtttagcgca                                         >  SRR4302536.3045485/1‑77 (MQ=60)

                                                      AGACTCAAGCGCCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCGCAGTACcgggta                                         >  SRR4302536.5379030/1‑88 (MQ=60)

                                                       GACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCAC                                        >  SRR4302536.1952810/1‑94 (MQ=60)

                                                          TCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCA                                     >  SRR4302536.6509858/1‑94 (MQ=60)

                                                           CCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAG                                    <  SRR4302536.2245973/94‑1 (MQ=60)

                                                              GCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCC                                 <  SRR4302536.2019170/94‑1 (MQ=60)

                                                              GCGGCGCCCGTATAATGTGATTAGCGTTCAC‑TCCACGAGTCGGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCC                                 <  SRR4302536.25290/94‑1 (MQ=60)

                                                               CGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCG                                >  SRR4302536.2489097/1‑94 (MQ=60)

                                                               CGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCG                                >  SRR4302536.931694/1‑94 (MQ=60)

                                                               CGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCG                                >  SRR4302536.4507862/1‑94 (MQ=60)

                                                               CGTCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCA                                <  SRR4302536.3742161/94‑1 (MQ=60)

                                                                 TCGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGT                              <  SRR4302536.1835005/94‑1 (MQ=60)

                                                                  CGCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTA                             >  SRR4302536.1854208/1‑94 (MQ=60)

                                                                   GCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAA                            <  SRR4302536.1143925/94‑1 (MQ=60)

                                                                   GCGCGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAA                            >  SRR4302536.4838065/1‑94 (MQ=60)

                                                                     GCGTATAATGTGGTTAGCGTGCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAAT                          <  SRR4302536.4016645/94‑1 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         >  SRR4302536.1839035/1‑94 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         <  SRR4302536.3092618/94‑1 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         <  SRR4302536.3502579/94‑1 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         <  SRR4302536.731380/94‑1 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         <  SRR4302536.4868635/94‑1 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         >  SRR4302536.961238/1‑94 (MQ=60)

                                                                      CGTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATG                         <  SRR4302536.4587569/94‑1 (MQ=60)

                                                                       TTATAATGTGGTTAGCGTTCAC‑TCCAGGAGTCGGACCTAAGATCGCCCCGCGCGCATATGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGC                        <  SRR4302536.348204/94‑1 (MQ=60)

                                                                       GTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGC                        <  SRR4302536.5159091/94‑1 (MQ=60)

                                                                       GTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGC                        <  SRR4302536.5772371/94‑1 (MQ=60)

                                                                       GTATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGC                        >  SRR4302536.3697268/1‑94 (MQ=60)

                                                                        TATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCC                       >  SRR4302536.4992669/1‑94 (MQ=60)

                                                                        TATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCC                       >  SRR4302536.2920214/1‑94 (MQ=60)

                                                                         ATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCG                      <  SRR4302536.6590049/94‑1 (MQ=60)

                                                                         ATAATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCG                      >  SRR4302536.4024670/1‑94 (MQ=60)

                                                                          AAATGTGGTAAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGG                     <  SRR4302536.640057/94‑1 (MQ=60)

                                                                           AATGTGGTTAGCGTTCGC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGA                    >  SRR4302536.1626217/1‑94 (MQ=60)

                                                                           AATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGA                    >  SRR4302536.6284245/1‑94 (MQ=60)

                                                                            ATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAA                   <  SRR4302536.3234844/94‑1 (MQ=60)

                                                                            ATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAA                   <  SRR4302536.6789794/94‑1 (MQ=60)

                                                                            ATGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAA                   >  SRR4302536.4910235/1‑94 (MQ=60)

                                                                             TGTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAAC                  >  SRR4302536.5186879/1‑94 (MQ=60)

                                                                              GTGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACC                 <  SRR4302536.3574650/94‑1 (MQ=60)

                                                                               TGGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCA                <  SRR4302536.1846947/94‑1 (MQ=60)

                                                                                GGTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCAC               >  SRR4302536.1146380/1‑94 (MQ=60)

                                                                                 GTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACA              >  SRR4302536.4394036/1‑94 (MQ=60)

                                                                                 GTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACA              >  SRR4302536.5417196/1‑94 (MQ=60)

                                                                                 GTTAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACA              >  SRR4302536.6839340/1‑94 (MQ=60)

                                                                                   TAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATA            <  SRR4302536.4069102/94‑1 (MQ=60)

                                                                                   TAGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATA            <  SRR4302536.2263856/94‑1 (MQ=60)

                                                                                    AGCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATAC           >  SRR4302536.505219/1‑94 (MQ=60)

                                                                                     GCGTTCAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTCAATGCCGGAACCACATACA          >  SRR4302536.2064069/1‑94 (MQ=60)

                                                                                          CAC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCA     <  SRR4302536.1429680/94‑1 (MQ=60)

                                                                                           AC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATATGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT    >  SRR4302536.3837951/1‑94 (MQ=60)

                                                                                           AC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT    >  SRR4302536.6843221/1‑94 (MQ=60)

                                                                                           AC‑TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCAT    >  SRR4302536.2656899/1‑94 (MQ=60)

                                                                                              TCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTG  <  SRR4302536.1164887/94‑1 (MQ=60)

GATACCAGAGTCGGCCTGCATCGCTTCCAGCACGTCACGCGTCTGATAAGCAATAGACTCCAGCGTCGCGCGTATAATGTGGTTAGCGTTCAC‑CCCACGAGTCAGACCGAAAATCGCCCCGCGCGCATACGGGTCCCAGTACGGCGCACCCAGCCCGGTAAATGCCGGAACCACATACACACCATTG  >  NC_000913/4113597‑4113783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`