Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,149,444 A→G Y412H (TAC→CAC)  ppc ← phosphoenolpyruvate carboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,149,4440AG97.7% 389.9 / 0.0 129Y412H (TAC→CAC) ppcphosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (65/61);  total (66/63)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-02

Only 100 of 136 total aligned reads displayed.

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533
                                                                                          |                                                                                         
gCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGc                                                                                         <  1:1058185/93‑1 (MQ=255)
gCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGc                                                                                         <  2:2351098/93‑1 (MQ=255)
gCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGc                                                                                         <  1:572089/93‑1 (MQ=255)
gCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGc                                                                                         >  1:2458145/1‑93 (MQ=255)
  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       <  2:1294281/93‑1 (MQ=255)
  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       >  1:2304328/1‑93 (MQ=255)
   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  2:3291872/1‑93 (MQ=255)
     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    >  1:4219643/1‑93 (MQ=255)
     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTc                                                                                    <  2:819803/93‑1 (MQ=255)
        ggACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCCGc                                                                                 >  2:2268579/1‑93 (MQ=255)
        ggACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGc                                                                                 <  2:2029599/93‑1 (MQ=255)
         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                <  1:2201983/93‑1 (MQ=255)
          aCGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTc                                                                               >  2:3980170/1‑93 (MQ=255)
           cGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCg                                                                              >  2:754066/1‑93 (MQ=255)
             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            <  1:2804365/93‑1 (MQ=255)
              ttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGccc                                                                           <  2:1531423/93‑1 (MQ=255)
              ttGGAGTTCAGTTCGCGGATCAGGAAAGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGacc                                                                           >  2:1923486/1‑93 (MQ=255)
                ggAGTTCAGTTCGCGGATCAGGAACTCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAg                                                                         <  2:1594571/93‑1 (MQ=255)
                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        <  2:3892150/93‑1 (MQ=255)
                 gAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgc                                                                        <  2:426021/93‑1 (MQ=255)
                    ttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCt                                                                     >  1:4487256/1‑93 (MQ=255)
                    ttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCt                                                                     >  2:470575/1‑93 (MQ=255)
                    ttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCt                                                                     >  2:4734417/1‑93 (MQ=255)
                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    >  2:3159198/1‑93 (MQ=255)
                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:822035/93‑1 (MQ=255)
                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:3971740/93‑1 (MQ=255)
                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:824585/93‑1 (MQ=255)
                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:2023793/93‑1 (MQ=255)
                        gttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCgg                                                                 >  2:3238726/1‑93 (MQ=255)
                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  1:2416373/93‑1 (MQ=255)
                            gcgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATg                                                             >  2:1148034/1‑93 (MQ=255)
                            gcgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTCGCGGGTCAGCTCGCCCAGCGCTTTGGTATg                                                             >  2:516359/1‑93 (MQ=255)
                              ggATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAc                                                           >  2:3755838/1‑93 (MQ=255)
                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          >  2:4064230/1‑93 (MQ=255)
                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  2:3096347/1‑93 (MQ=255)
                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         <  1:544592/93‑1 (MQ=255)
                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCCCGCCCAGCGCTTCGGTATGAcgc                                                         <  1:649151/93‑1 (MQ=255)
                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  2:2083020/1‑93 (MQ=255)
                                  cAGGAACGCCTGTTTGTCGGCTTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGt                                                       <  2:2338789/93‑1 (MQ=255)
                                  cAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGt                                                       >  1:1537007/1‑93 (MQ=255)
                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     <  1:1667929/93‑1 (MQ=255)
                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  1:3630662/1‑93 (MQ=255)
                                     gAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGct                                                    <  1:2065449/93‑1 (MQ=255)
                                      gaCGCCTGTTTGTCGGCCTCTGACCAGCTTGCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  1:3192211/92‑1 (MQ=255)
                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  1:1911824/93‑1 (MQ=255)
                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  1:3192212/93‑1 (MQ=255)
                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  2:3098766/93‑1 (MQ=255)
                                       aCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctct                                                  >  1:4697340/1‑93 (MQ=255)
                                       aCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctct                                                  >  2:3574348/1‑93 (MQ=255)
                                         gCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTcc                                                <  2:2730845/93‑1 (MQ=255)
                                         gCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTcc                                                >  1:4652860/1‑93 (MQ=255)
                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               >  1:3338026/1‑93 (MQ=255)
                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             >  2:1546506/1‑93 (MQ=255)
                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCGCGCCCAGCGCGTCGGTATGACGCGTGCTCTCCCGa                                             >  2:4218759/1‑93 (MQ=255)
                                              tttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACg                                           <  1:2973703/93‑1 (MQ=255)
                                              tttGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTAGGTATTACGCGTGCTCTCCTGACg                                           >  2:1473226/1‑93 (MQ=255)
                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  2:2305269/93‑1 (MQ=255)
                                                 gTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGat                                        >  1:3908336/1‑93 (MQ=255)
                                                 gTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGat                                        <  2:2860407/93‑1 (MQ=255)
                                                    ggCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATc                                     >  1:2974549/1‑93 (MQ=255)
                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  2:3994609/1‑93 (MQ=255)
                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    <  1:1176524/93‑1 (MQ=255)
                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  2:1999705/1‑93 (MQ=255)
                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  2:1671175/1‑93 (MQ=255)
                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  2:4653965/1‑93 (MQ=255)
                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  <  1:3239020/93‑1 (MQ=255)
                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             >  2:3482749/1‑93 (MQ=255)
                                                              cAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAca                           <  1:1012195/93‑2 (MQ=255)
                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATCTCACTACGGACCAGCg                       >  1:1668464/1‑93 (MQ=255)
                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCg                       >  2:4578442/1‑93 (MQ=255)
                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCg                       <  2:456488/93‑1 (MQ=255)
                                                                  tttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCg                       >  1:3237340/1‑93 (MQ=255)
                                                                   ttCGTAGTGGCCGATACCGAGGTGGGGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  2:3842820/93‑1 (MQ=255)
                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  1:170007/93‑1 (MQ=255)
                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  2:47791/93‑1 (MQ=255)
                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   >  1:4478851/1‑93 (MQ=255)
                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  2:4444422/1‑93 (MQ=255)
                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  1:3059924/1‑93 (MQ=255)
                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  1:3145/1‑93 (MQ=255)
                                                                        aGTCGCCTATCACGAGGTGGCGGGTGAGCTCGCCCAGCGCTTGGGTATGACCCGTGCTGTCCTGACGGATATCAATACGGACCAGCGGTACGc                 <  1:1886344/93‑1 (MQ=255)
                                                                        aGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGc                 <  1:1886345/93‑1 (MQ=255)
                                                                         gTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGcc                >  1:3804194/1‑93 (MQ=255)
                                                                             gcGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGaaa            <  2:2635126/92‑1 (MQ=255)
                                                                              cGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAc           >  2:1098678/1‑93 (MQ=255)
                                                                              cGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAc           <  1:3237005/93‑1 (MQ=255)
                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          >  2:4319696/1‑93 (MQ=255)
                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          <  1:667392/93‑1 (MQ=255)
                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          <  1:2923658/93‑1 (MQ=255)
                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          >  1:1350208/1‑93 (MQ=255)
                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  2:4360949/1‑93 (MQ=255)
                                                                                aTACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  2:1570157/1‑93 (MQ=255)
                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  1:3025725/93‑1 (MQ=255)
                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  1:1083644/93‑1 (MQ=255)
                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  2:581990/93‑1 (MQ=255)
                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  2:581991/93‑1 (MQ=255)
                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      >  2:2692790/1‑93 (MQ=255)
                                                                                    cGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCa     >  1:4094210/1‑93 (MQ=255)
                                                                                     gAGGTGGCGGGTCAGCTCGCCCCGCCGTTCCGTATGAGGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  1:34288/93‑1 (MQ=255)
                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  1:4491826/1‑93 (MQ=255)
                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  1:2344311/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG  >  NC_000913/4149353‑4149532
                                                                                           |                                                                                        
GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGC                                                                                        >  SRR4302544.2481595/1‑94 (MQ=60)
GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGC                                                                                        <  SRR4302544.421083/94‑1 (MQ=60)
 GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCG                                                                                       <  SRR4302544.1068691/94‑1 (MQ=60)
 GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCG                                                                                       <  SRR4302544.2156261/94‑1 (MQ=60)
 GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCG                                                                                       <  SRR4302544.577682/94‑1 (MQ=60)
  CAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGG                                                                                      >  SRR4302544.797467/1‑94 (MQ=60)
  CAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGG                                                                                      >  SRR4302544.2326098/1‑94 (MQ=60)
     AAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTC                                                                                   >  SRR4302544.4262370/1‑94 (MQ=60)
          GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                              <  SRR4302544.2222730/94‑1 (MQ=60)
          GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                              <  SRR4302544.554597/94‑1 (MQ=60)
              TTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCC                                                                          <  SRR4302544.2831403/94‑1 (MQ=60)
               TTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCA                                                                         <  SRR4302544.3145658/94‑1 (MQ=60)
                    GTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCT                                                                    >  SRR4302544.4532330/1‑94 (MQ=60)
                       CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                 <  SRR4302544.830070/94‑1 (MQ=60)
                       CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                 <  SRR4302544.2043423/94‑1 (MQ=60)
                       CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                 <  SRR4302544.4011248/94‑1 (MQ=60)
                       CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                 <  SRR4302544.832663/94‑1 (MQ=60)
                           TCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTAT                                                             <  SRR4302544.302683/94‑1 (MQ=60)
                           TCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTAT                                                             <  SRR4302544.2439372/94‑1 (MQ=60)
                               GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                         >  SRR4302544.2380658/1‑94 (MQ=60)
                                 ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                       <  SRR4302544.549945/94‑1 (MQ=60)
                                 ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                       <  SRR4302544.1187679/94‑1 (MQ=60)
                                 ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                       >  SRR4302544.41105/1‑94 (MQ=60)
                                 ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCCCGCCCAGCGCTTCGGTATGACGCG                                                       <  SRR4302544.655565/94‑1 (MQ=60)
                                  TCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGT                                                      >  SRR4302544.1552280/1‑94 (MQ=60)
                                    AGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGC                                                    >  SRR4302544.3666835/1‑94 (MQ=60)
                                     GGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCT                                                   <  SRR4302544.1684353/94‑1 (MQ=60)
                                      GAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                  <  SRR4302544.2085297/94‑1 (MQ=60)
                                      GAACGCCTGTTTGTCGGCCTCTGACAAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTC                                                  <  SRR4302544.4541707/94‑1 (MQ=60)
                                       GACGCCTGTTTGTCGGCCTCTGACCAGCTTGCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                 <  SRR4302544.3222628/94‑1 (MQ=60)
                                       AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                 >  SRR4302544.4743712/1‑94 (MQ=60)
                                       AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                 <  SRR4302544.3222629/94‑1 (MQ=60)
                                       AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                 <  SRR4302544.1930298/94‑1 (MQ=60)
                                         CGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCC                                               >  SRR4302544.4698906/1‑94 (MQ=60)
                                          GCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCT                                              >  SRR4302544.3369832/1‑94 (MQ=60)
                                           CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                             <  SRR4302544.1822802/94‑1 (MQ=60)
                                            CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGGTACCGAGGTGGCGGGGCAGCTCGACCAGCGCCTCGGTATGCCGCGAGCTCTCCTAA                                            >  SRR4302544.712558/1‑94 (MQ=60)
                                               TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGG                                         <  SRR4302544.3002224/94‑1 (MQ=60)
                                                 TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                       >  SRR4302544.3947168/1‑94 (MQ=60)
                                                   TCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATAT                                     <  SRR4302544.3254213/94‑1 (MQ=60)
                                                    CGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATC                                    >  SRR4302544.3003082/1‑94 (MQ=60)
                                                     GGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGGTCTCCTGACGGATATCA                                   >  SRR4302544.2950244/1‑94 (MQ=60)
                                                      GCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAA                                  <  SRR4302544.1188250/94‑1 (MQ=60)
                                                       CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                 <  SRR4302544.4379164/94‑1 (MQ=60)
                                                        CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                <  SRR4302544.3270021/94‑1 (MQ=60)
                                                               CAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACAA                         <  SRR4302544.1022284/94‑1 (MQ=60)
                                                                  CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATCTCACTACGGACCAGCG                      >  SRR4302544.1684902/1‑94 (MQ=60)
                                                                  CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                      >  SRR4302544.3268327/1‑94 (MQ=60)
                                                                   TTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGG                     >  SRR4302544.327914/1‑94 (MQ=60)
                                                                      CGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAC                  >  SRR4302544.4523876/1‑94 (MQ=60)
                                                                       GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                 >  SRR4302544.3173/1‑94 (MQ=60)
                                                                       GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                 >  SRR4302544.3089125/1‑94 (MQ=60)
                                                                       GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                 <  SRR4302544.171683/94‑1 (MQ=60)
                                                                         AGTCGCCTATCACGAGGTGGCGGGTGAGCTCGCCCAGCGCTTGGGTATGACCCGTGCTGTCCTGACGGATATCAATACGGACCAGCGGTACGCC               <  SRR4302544.1904573/94‑1 (MQ=60)
                                                                         AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCC               >  SRR4302544.3841889/1‑94 (MQ=60)
                                                                         AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCC               <  SRR4302544.1904574/94‑1 (MQ=60)
                                                                              CCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAC          <  SRR4302544.3823580/94‑1 (MQ=60)
                                                                               CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA         >  SRR4302544.1978770/1‑94 (MQ=60)
                                                                               CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA         <  SRR4302544.3267992/94‑1 (MQ=60)
                                                                               CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA         >  SRR4302544.1363589/1‑94 (MQ=60)
                                                                               CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA         <  SRR4302544.1672981/94‑1 (MQ=60)
                                                                                GATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAT        <  SRR4302544.2951762/94‑1 (MQ=60)
                                                                                GATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAT        <  SRR4302544.673981/94‑1 (MQ=60)
                                                                                  TACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTT      <  SRR4302544.3054714/94‑1 (MQ=60)
                                                                                    CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA    <  SRR4302544.1094423/94‑1 (MQ=60)
                                                                                    CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA    <  SRR4302544.837786/94‑1 (MQ=60)
                                                                                    CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA    >  SRR4302544.4134946/1‑94 (MQ=60)
                                                                                      GAGGTGGCGGGTCAGCTCGCCCCGCCGTTCCGTATGAGGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG  <  SRR4302544.34626/94‑1 (MQ=60)
                                                                                      GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG  >  SRR4302544.4536918/1‑94 (MQ=60)
                                                                                      GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG  >  SRR4302544.2366519/1‑94 (MQ=60)
                                                                                           |                                                                                        
GGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG  >  NC_000913/4149353‑4149532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑