Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,149,444	A→G	Y412H (TAC→CAC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,149,444	0	A	G	100.0%	785.1 / NA	245	Y412H (TAC→CAC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (130/115); total (130/115)

Only 100 of 253 total aligned reads displayed.

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

 cAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCg                                                                                        >  2:3217311/1‑93 (MQ=255)

  aGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCgg                                                                                       <  2:5063960/93‑1 (MQ=255)

   gAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCggg                                                                                      >  1:583136/1‑93 (MQ=255)

    aaGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGt                                                                                     <  2:1159684/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    >  2:1419600/1‑93 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:5431415/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:293575/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTc                                                                                    <  2:5520847/93‑1 (MQ=255)

     aGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTATCGTAGTCGCCGATACCGAGGTGGCGGCTc                                                                                    >  2:2726142/1‑93 (MQ=255)

       cGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAg                                                                                  >  1:6032065/1‑93 (MQ=255)

        ggACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGc                                                                                 >  1:6595490/1‑93 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  1:6194346/1‑93 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                <  1:5382489/93‑1 (MQ=255)

         gACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                <  1:3030526/93‑1 (MQ=255)

         gACGTTAGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCt                                                                                >  1:5555093/1‑93 (MQ=255)

            gTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGc                                                                             >  1:2375829/1‑93 (MQ=255)

             tttGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGcc                                                                            >  2:1932908/1‑93 (MQ=255)

                ggAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAg                                                                         >  1:4699786/1‑93 (MQ=255)

                  agttcagttcGCGGATCAGGAACGCCTGTTTATCGGCCTCTGACCAGCTTTCGTAGGCGCCGATACCGAGGTGGAGGGGCACCCCGcccctcg                                                                       >  1:192324/1‑89 (MQ=255)

                   gttcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAgcgc                                                                      <  1:5899903/93‑1 (MQ=255)

                     tcagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCtt                                                                    <  1:1344143/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  1:6479886/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  2:477588/93‑1 (MQ=255)

                      cagttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTc                                                                   <  2:1231529/93‑1 (MQ=255)

                        gttcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCgg                                                                 >  2:3817056/1‑93 (MQ=255)

                          tcGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTa                                                               <  2:460145/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:3139273/93‑1 (MQ=255)

                             cgGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGa                                                            <  2:6517077/93‑1 (MQ=255)

                              ggATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAc                                                           >  2:1814603/1‑93 (MQ=255)

                               gATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcg                                                          >  1:4349877/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  2:340182/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  1:4050237/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         <  2:3543867/93‑1 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         >  1:4802872/1‑93 (MQ=255)

                                aTCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgc                                                         <  1:9687/93‑1 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  2:5839626/1‑93 (MQ=255)

                                 tCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAcgcg                                                        >  2:2523348/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  1:3234866/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  2:5950083/1‑93 (MQ=255)

                                    ggAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGc                                                     >  2:4047873/1‑93 (MQ=255)

                                      aaCGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctc                                                   <  2:4335005/93‑1 (MQ=255)

                                       aCGCGTGTTTGTCGTCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGctct                                                  <  1:5691452/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  1:6667055/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               >  2:1683045/1‑93 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  2:6707928/93‑1 (MQ=255)

                                          ccTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCt                                               <  1:5105839/93‑1 (MQ=255)

                                           cTGTTTGTCGGTCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  2:5245270/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  1:2490931/1‑93 (MQ=255)

                                           cTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTg                                              >  1:1915556/1‑93 (MQ=255)

                                            tGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGa                                             <  2:4578155/93‑1 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            >  1:5205192/1‑93 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            >  2:3054764/1‑93 (MQ=255)

                                             gTTTGTCGGCCTCTGACCAGCTTTCGAAGTCGCCGATACCGAGGTGGCGAGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAc                                            <  2:1489328/93‑1 (MQ=255)

                                                tGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGa                                         <  1:226119/93‑1 (MQ=255)

                                                  tCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGata                                       >  2:435463/1‑93 (MQ=255)

                                                  gcGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTGCGGTATGACGCGTGCTCTCCTGACGGata                                       <  1:3079697/92‑1 (MQ=255)

                                                    ggCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATc                                     <  1:6388441/93‑1 (MQ=255)

                                                     gCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCa                                    >  2:3976663/1‑93 (MQ=255)

                                                      ccTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCaa                                   >  1:2085915/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  1:4542470/1‑93 (MQ=255)

                                                       ctctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAt                                  >  1:884458/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 >  1:5986395/1‑93 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 <  1:1665809/93‑1 (MQ=255)

                                                        tctGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATa                                 >  2:2065364/1‑93 (MQ=255)

                                                          tGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACg                               <  2:2462906/93‑1 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             >  1:6303294/1‑93 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             <  1:2577616/93‑1 (MQ=255)

                                                            aCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGa                             <  1:6008191/93‑1 (MQ=255)

                                                               aGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCa                          <  1:1469473/93‑1 (MQ=255)

                                                                gCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAg                         >  1:2789114/1‑93 (MQ=255)

                                                                 cTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGc                        <  1:3373094/93‑1 (MQ=255)

                                                                 cTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGc                        <  1:4214919/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGGTATCAATACGCACCAGCgg                      <  1:6554586/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  1:220566/93‑1 (MQ=255)

                                                                   ttCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCgg                      <  2:4166950/93‑1 (MQ=255)

                                                                   atcttAGTCGCCGATACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGTATATCAATACGGTCCAGCgg                      <  1:4468238/89‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  2:77734/93‑1 (MQ=255)

                                                                     cGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTa                    <  1:3406955/93‑1 (MQ=255)

                                                                      gTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAc                   <  1:6211051/93‑1 (MQ=255)

                                                                       tAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACg                  >  2:6071659/1‑93 (MQ=255)

                                                                        aGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGc                 >  1:1094212/1‑93 (MQ=255)

                                                                         gTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGcc                >  1:3093714/1‑93 (MQ=255)

                                                                         gTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGcc                >  2:5682626/1‑93 (MQ=255)

                                                                           cGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGa              >  2:6415261/1‑93 (MQ=255)

                                                                              cGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAc           <  1:2781028/93‑1 (MQ=255)

                                                                               gATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACa          >  1:1579735/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  1:743432/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         >  1:4621600/1‑93 (MQ=255)

                                                                                aTACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAt         <  2:3973274/93‑1 (MQ=255)

                                                                                aTACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGGACGCCGAAACAt         >  1:3915012/1‑93 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  2:5705865/93‑1 (MQ=255)

                                                                                 tACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAtt        <  1:1236151/93‑1 (MQ=255)

                                                                                 tACCGAGGGGGCGGGTCAGCTCGCCCAGCGCTTCGGGATGACGCGTGCTCTCCTGACGGGTATGAATACGGACCAGCGGTACCCCGAAACAtt        >  2:4948632/1‑93 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      <  1:3699647/93‑1 (MQ=255)

                                                                                   ccGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTc      >  2:1124225/1‑93 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGGCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATCTCAATACGGACCAGCGGTACCCCGAAACCTTTAAc    >  1:4149907/1‑93 (MQ=255)

                                                                                     gAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAc    <  1:5487829/93‑1 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   <  2:4932183/93‑1 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   >  2:1091950/1‑93 (MQ=255)

                                                                                      aGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCAcg   <  2:3483562/93‑1 (MQ=255)

GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGC  >  NC_000913/4149354‑4149533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  >  NC_000913/4149351‑4149537

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTG                                                                                               <  SRR4302536.6879903/94‑1 (MQ=60)

 CGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGG                                                                                              <  SRR4302536.6855005/94‑1 (MQ=60)

 CGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGG                                                                                              <  SRR4302536.2029748/94‑1 (MQ=60)

   GCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCG                                                                                            <  SRR4302536.5502049/94‑1 (MQ=60)

     AGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGG                                                                                          >  SRR4302536.597978/1‑94 (MQ=60)

      GAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGT                                                                                         >  SRR4302536.6389834/1‑94 (MQ=60)

         GCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAG                                                                                      >  SRR4302536.6211934/1‑94 (MQ=60)

          CGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGC                                                                                     >  SRR4302536.6791688/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    <  SRR4302536.2541148/94‑1 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302536.6911381/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302536.6167804/1‑94 (MQ=60)

           GGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302536.6378851/1‑94 (MQ=60)

           GGACGTTAGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCT                                                                                    >  SRR4302536.5720556/1‑94 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   <  SRR4302536.1256976/94‑1 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   <  SRR4302536.5543137/94‑1 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   <  SRR4302536.6110277/94‑1 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   <  SRR4302536.3145801/94‑1 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   >  SRR4302536.2779811/1‑94 (MQ=60)

            GACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTC                                                                                   <  SRR4302536.3119575/94‑1 (MQ=60)

              CGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGC                                                                                 >  SRR4302536.2447807/1‑94 (MQ=60)

              CGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGC                                                                                 >  SRR4302536.22714/1‑94 (MQ=60)

               GTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCC                                                                                >  SRR4302536.635870/1‑94 (MQ=60)

               GTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCC                                                                                >  SRR4302536.6794711/1‑94 (MQ=60)

                 TTGGAGTTCAGTTCGCGGATCAGCAACGCCTGTTTGTCGGCCTCTGTCCAGCTTTCGTAGTCGCCGATACCGAGGTGGCCGGTCAGCTCGCCCA                                                                              <  SRR4302536.557835/94‑1 (MQ=60)

                  TGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAG                                                                             >  SRR4302536.4841099/1‑94 (MQ=60)

                   GGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGC                                                                            <  SRR4302536.2272704/94‑1 (MQ=60)

                     AGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGC                                                                          >  SRR4302536.581319/1‑94 (MQ=60)

                     AGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGC                                                                          >  SRR4302536.1628527/1‑94 (MQ=60)

                     AGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGC                                                                          >  SRR4302536.6422252/1‑94 (MQ=60)

                      GTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCT                                                                         <  SRR4302536.6075571/94‑1 (MQ=60)

                       TTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTT                                                                        <  SRR4302536.4758275/94‑1 (MQ=60)

                        TCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTC                                                                       <  SRR4302536.1384221/94‑1 (MQ=60)

                         CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                      >  SRR4302536.2197034/1‑94 (MQ=60)

                         CAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCG                                                                      <  SRR4302536.6673196/94‑1 (MQ=60)

                          AGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGG                                                                     >  SRR4302536.3828842/1‑94 (MQ=60)

                            TTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTA                                                                   <  SRR4302536.5777728/94‑1 (MQ=60)

                             TCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTAT                                                                  <  SRR4302536.5272580/94‑1 (MQ=60)

                               GCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGA                                                                >  SRR4302536.879891/1‑94 (MQ=60)

                                CGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGAC                                                               <  SRR4302536.6791431/94‑1 (MQ=60)

                                CGGATCAGGAACGCCTGTTGGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCGCGCCCAGCGCTTCGGTATGAC                                                               <  SRR4302536.1483898/94‑1 (MQ=60)

                                 GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                              <  SRR4302536.6604459/94‑1 (MQ=60)

                                 GGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACG                                                              >  SRR4302536.4480036/1‑94 (MQ=60)

                                  GATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGC                                                             >  SRR4302536.4947417/1‑94 (MQ=60)

                                  GATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGC                                                             >  SRR4302536.4781200/1‑94 (MQ=60)

                                  GATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGC                                                             >  SRR4302536.4170304/1‑94 (MQ=60)

                                   ATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCG                                                            <  SRR4302536.9963/94‑1 (MQ=60)

                                     CAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTG                                                          >  SRR4302536.4614396/1‑94 (MQ=60)

                                      AGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGC                                                         >  SRR4302536.3329693/1‑94 (MQ=60)

                                         AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                      >  SRR4302536.6691811/1‑94 (MQ=60)

                                         AACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCT                                                      >  SRR4302536.6691813/1‑94 (MQ=60)

                                          ACGCGTGTTTGTCGTCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTC                                                     <  SRR4302536.5860681/94‑1 (MQ=60)

                                           CGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCC                                                    <  SRR4302536.4236624/94‑1 (MQ=60)

                                            GCCTGTTTGTCGTCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGaaagctggt                                                   <  SRR4302536.3192950/94‑10 (MQ=60)

                                            GCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTTCT                                                   >  SRR4302536.450172/1‑94 (MQ=60)

                                            GCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTAGGACGCGTGCTCTCCT                                                   <  SRR4302536.4184565/94‑1 (MQ=60)

                                            CCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCT                                                   <  SRR4302536.1342517/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  <  SRR4302536.1619026/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  <  SRR4302536.5258455/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  <  SRR4302536.2015993/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  <  SRR4302536.6865179/94‑1 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  >  SRR4302536.1973166/1‑94 (MQ=60)

                                             CCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTG                                                  >  SRR4302536.2566072/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302536.3493945/1‑94 (MQ=60)

                                              CTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGA                                                 >  SRR4302536.675501/1‑94 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTGAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                <  SRR4302536.6655703/94‑1 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                >  SRR4302536.5360352/1‑94 (MQ=60)

                                               TGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGAC                                                >  SRR4302536.6419437/1‑94 (MQ=60)

                                                 TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGG                                              >  SRR4302536.4314611/1‑94 (MQ=60)

                                                 TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGG                                              >  SRR4302536.5142708/1‑94 (MQ=60)

                                                 TTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGG                                              <  SRR4302536.6048537/94‑1 (MQ=60)

                                                  TTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGA                                             >  SRR4302536.6051073/1‑94 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302536.232135/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302536.3951777/94‑1 (MQ=60)

                                                   TGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGAT                                            <  SRR4302536.196965/94‑1 (MQ=60)

                                                   TGTCGCCCTCAGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTAAGCTCACCCAGCGCTTCGGTATGACGCGTCCTCTCCTGACGGAT                                            <  SRR4302536.1618156/94‑1 (MQ=60)

                                                     TCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATAT                                          <  SRR4302536.3337526/94‑1 (MQ=60)

                                                     TCGGCCTCTGACCAGCTCTCGTAGTCGCCGATACCTAGGTGGCGGGTCAGCCCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATAT                                          <  SRR4302536.4470856/94‑1 (MQ=60)

                                                     GCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTGCGGTATGACGCGTGCTCTCCTGACGGATAT                                          <  SRR4302536.3169841/94‑1 (MQ=60)

                                                      CGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATC                                         >  SRR4302536.1871381/1‑94 (MQ=60)

                                                       GGCCTCTGCCCAGCTTTCGTCGTCTCCGATACCGAGGTGGCGGGTCACCTCGCCCAGCGCTTCGGTATGACGCGTGCTATCCTGACGGATATCA                                        <  SRR4302536.4459843/94‑1 (MQ=60)

                                                       GGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCA                                        <  SRR4302536.28824/94‑1 (MQ=60)

                                                       GGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCA                                        <  SRR4302536.6579043/94‑1 (MQ=60)

                                                       GGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCA                                        <  SRR4302536.6211785/94‑1 (MQ=60)

                                                       GGCCTCTGACCAGCTTTCGTAGTCGCCGAGACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCA                                        <  SRR4302536.6792918/94‑1 (MQ=60)

                                                        GCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAA                                       >  SRR4302536.2148704/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302536.863878/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302536.4678677/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302536.566868/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302536.910489/1‑94 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      <  SRR4302536.2865929/94‑1 (MQ=60)

                                                         CCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGAGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAAT                                      >  SRR4302536.161288/1‑94 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     <  SRR4302536.3067853/94‑1 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     >  SRR4302536.6164881/1‑94 (MQ=60)

                                                          CTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATA                                     >  SRR4302536.1774818/1‑94 (MQ=60)

                                                           TCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATTC                                    <  SRR4302536.6680260/94‑1 (MQ=60)

                                                           TCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAC                                    <  SRR4302536.2440496/94‑1 (MQ=60)

                                                           TCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAC                                    <  SRR4302536.1714143/94‑1 (MQ=60)

                                                            CTGACCAGCTTTCGTGGTCGCCGATACCGAGGTGGCGGGTCAGCTAGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACG                                   <  SRR4302536.4119394/94‑1 (MQ=60)

                                                              GACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGA                                 >  SRR4302536.6491052/1‑94 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302536.6187305/94‑1 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302536.2524188/94‑1 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                >  SRR4302536.3179838/1‑94 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302536.2655108/94‑1 (MQ=60)

                                                               ACCAGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGAC                                <  SRR4302536.4809662/94‑1 (MQ=60)

                                                                  AGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAG                             <  SRR4302536.1512945/94‑1 (MQ=60)

                                                                  AGCTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAG                             >  SRR4302536.2871918/1‑94 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           <  SRR4302536.3473302/94‑1 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           <  SRR4302536.1283139/94‑1 (MQ=60)

                                                                    CTTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCG                           <  SRR4302536.4340262/94‑1 (MQ=60)

                                                                     TTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGG                          <  SRR4302536.5254331/94‑1 (MQ=60)

                                                                     TTTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATAAGGACCAGCGG                          >  SRR4302536.2597735/1‑94 (MQ=60)

                                                                      atctTAGTCGCCGATACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGTATATCAATACGGTCCAGCGGT                         <  SRR4302536.4602467/90‑1 (MQ=60)

                                                                      TTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGGTATCAATACGCACCAGCGGT                         <  SRR4302536.6749764/94‑1 (MQ=60)

                                                                      TTCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGT                         <  SRR4302536.226419/94‑1 (MQ=60)

                                                                       TCGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTA                        <  SRR4302536.487340/94‑1 (MQ=60)

                                                                        CGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAC                       >  SRR4302536.5611646/1‑94 (MQ=60)

                                                                        CGTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTAC                       <  SRR4302536.3508292/94‑1 (MQ=60)

                                                                         GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                      >  SRR4302536.6506357/1‑94 (MQ=60)

                                                                         GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                      <  SRR4302536.6396103/94‑1 (MQ=60)

                                                                         GTAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCACAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACG                      >  SRR4302536.3492537/1‑94 (MQ=60)

                                                                          TAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGC                     >  SRR4302536.6168078/1‑94 (MQ=60)

                                                                          TAGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGC                     >  SRR4302536.1127043/1‑94 (MQ=60)

                                                                           AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCCATACGGACCAGCGGTACGCC                    >  SRR4302536.4651542/1‑94 (MQ=60)

                                                                           AGTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCC                    >  SRR4302536.3184121/1‑94 (MQ=60)

                                                                           AGTAGCCGATACCGAGGAGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGCTATCCATACGGCCCAGGGGTTCGCC                    >  SRR4302536.1931790/1‑94 (MQ=60)

                                                                            GTCGCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCG                   <  SRR4302536.5308616/94‑1 (MQ=60)

                                                                               GCCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAA                >  SRR4302536.3311804/1‑94 (MQ=60)

                                                                                CCGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAAC               <  SRR4302536.2033445/94‑1 (MQ=60)

                                                                                 CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA              <  SRR4302536.972789/94‑1 (MQ=60)

                                                                                 CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA              >  SRR4302536.1626011/1‑94 (MQ=60)

                                                                                 CGATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACA              <  SRR4302536.2863659/94‑1 (MQ=60)

                                                                                  GATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAT             >  SRR4302536.765252/1‑94 (MQ=60)

                                                                                  GATACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACAT             >  SRR4302536.4760462/1‑94 (MQ=60)

                                                                                  GATACCGAGGTGGCGGGGCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGGACGCCGAAACAT             >  SRR4302536.4031394/1‑94 (MQ=60)

                                                                                   ATAGCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATT            <  SRR4302536.5622413/94‑1 (MQ=60)

                                                                                    TACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTT           <  SRR4302536.2794894/94‑1 (MQ=60)

                                                                                    TACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTT           <  SRR4302536.1273274/94‑1 (MQ=60)

                                                                                     ACCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGGATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTC          >  SRR4302536.3121144/1‑94 (MQ=60)

                                                                                      CCGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCA         <  SRR4302536.3810282/94‑1 (MQ=60)

                                                                                       CGAGGTGGCGGGTCAGCTCGGCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATCTCAATACGGACCAGCGGTACCCCGAAACCTTTAAC        >  SRR4302536.4273063/1‑94 (MQ=60)

                                                                                       CGAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGCGCTCTCCTGACGGATCTCAACACGGTCCAGAGGTACGCCGAAACATTTAAC        >  SRR4302536.3940403/1‑94 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       <  SRR4302536.3705926/94‑1 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       <  SRR4302536.5651656/94‑1 (MQ=60)

                                                                                        GAGGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACG       <  SRR4302536.5861539/94‑1 (MQ=60)

                                                                                          GGTGGCGGGTCAGCTCGCCCAGCGCTTCGTTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCG     <  SRR4302536.3246435/94‑1 (MQ=60)

                                                                                          GGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCG     >  SRR4302536.5630419/1‑94 (MQ=60)

                                                                                          GGTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCG     <  SRR4302536.6373790/94‑1 (MQ=60)

                                                                                           GTGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGG    <  SRR4302536.4052672/94‑1 (MQ=60)

                                                                                            TGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGC   <  SRR4302536.4757051/94‑1 (MQ=60)

                                                                                            TGGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGC   >  SRR4302536.5150895/1‑94 (MQ=60)

                                                                                             GGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  >  SRR4302536.5405075/1‑94 (MQ=60)

                                                                                             GGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  <  SRR4302536.5939252/94‑1 (MQ=60)

GCGGCAGAAGCGGACGTTTGGAGTTCAGTTCGCGGATCAGGAACGCCTGTTTGTCGGCCTCTGACCAGCTTTCGTAGTCGCCGATACCGAGGTAGCGGGTCAGCTCGCCCAGCGCTTCGGTATGACGCGTGCTCTCCTGACGGATATCAATACGGACCAGCGGTACGCCGAAACATTTCACGCGGCG  >  NC_000913/4149351‑4149537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`