Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,173,376 A→G noncoding (44/76 nt) thrT → tRNA‑Thr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,173,3760AG98.8% 547.8 / ‑3.0 174noncoding (44/76 nt)thrTtRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (76/95);  total (76/98)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

Only 100 of 179 total aligned reads displayed.

TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT  >  NC_000913/4173284‑4173465
                                                                                            |                                                                                         
   cAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGt                                                                                        <  1:2560376/93‑1 (MQ=255)
    aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                       >  2:312398/1‑93 (MQ=255)
    aaGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTc                                                                                       >  2:3058806/1‑93 (MQ=255)
     aGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCg                                                                                      >  1:484836/1‑93 (MQ=255)
      gCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCgg                                                                                     >  1:3900478/1‑93 (MQ=255)
       cTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGc                                                                                    <  1:1985024/93‑1 (MQ=255)
        tgatgaTGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCa                                                                                   >  2:246609/1‑93 (MQ=255)
         gatgatGCGGGTTCGATTCCCGCTGCCCGCTCCAAAATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAg                                                                                  >  2:3483609/1‑93 (MQ=255)
            gatgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTc                                                                               <  1:4420961/93‑1 (MQ=255)
            gatgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTc                                                                               <  1:2845707/93‑1 (MQ=255)
              tgCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGa                                                                             <  2:1569417/93‑1 (MQ=255)
               gCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGaa                                                                            >  2:2347441/1‑93 (MQ=255)
                 gggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATc                                                                          <  1:4162283/93‑1 (MQ=255)
                 gggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATc                                                                          >  1:3136983/1‑93 (MQ=255)
                  ggTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCt                                                                         <  1:3716280/93‑1 (MQ=255)
                    ttCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGc                                                                       <  1:2058038/93‑1 (MQ=255)
                     tCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGcc                                                                      <  2:3962326/93‑1 (MQ=255)
                     tCGATGCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGcc                                                                      <  1:4261679/93‑1 (MQ=255)
                        aTTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTAt                                                                   <  2:4238653/93‑1 (MQ=255)
                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  >  1:2889462/1‑93 (MQ=255)
                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  <  1:164329/93‑1 (MQ=255)
                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  <  1:3127646/93‑1 (MQ=255)
                         ttCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATc                                                                  >  1:2730805/1‑93 (MQ=255)
                          tCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCa                                                                 <  2:1275192/93‑1 (MQ=255)
                          tCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCa                                                                 <  2:46003/93‑1 (MQ=255)
                           cccGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAg                                                                >  1:3725569/1‑93 (MQ=255)
                            ccGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGGTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGc                                                               >  1:3102761/1‑93 (MQ=255)
                             cGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGca                                                              <  2:730454/93‑1 (MQ=255)
                             cGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGca                                                              >  2:2117088/1‑93 (MQ=255)
                              gCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcac                                                             >  1:3515472/1‑93 (MQ=255)
                              gCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGcac                                                             <  2:3999758/93‑1 (MQ=255)
                                   ccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActt                                                        <  2:3023721/93‑1 (MQ=255)
                                   ccGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActt                                                        <  2:1252624/93‑1 (MQ=255)
                                    cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtc                                                       >  2:406330/1‑93 (MQ=255)
                                    cGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtc                                                       <  1:3556592/93‑1 (MQ=255)
                                     gCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtct                                                      <  2:2172032/93‑1 (MQ=255)
                                      cTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCActtctt                                                     >  1:481813/1‑93 (MQ=255)
                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    >  2:2736935/1‑93 (MQ=255)
                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    >  2:1595543/1‑93 (MQ=255)
                                       tCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCttt                                                    <  2:1310639/93‑1 (MQ=255)
                                         cAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtc                                                  >  2:285362/1‑93 (MQ=255)
                                          aaGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtct                                                 >  1:428555/1‑93 (MQ=255)
                                          aaGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtct                                                 <  1:1401979/93‑1 (MQ=255)
                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                >  1:4307710/1‑93 (MQ=255)
                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                >  2:1604037/1‑93 (MQ=255)
                                           aGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTtctc                                                <  1:682699/93‑1 (MQ=255)
                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  2:1642588/93‑1 (MQ=255)
                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  2:605240/93‑1 (MQ=255)
                                            gATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcc                                               <  1:3034458/93‑1 (MQ=255)
                                            gATGTGCTGATATAGCTAAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCCCTTCTTTTctcc                                               <  2:3142879/93‑1 (MQ=21)
                                             aTGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTctcct                                              >  2:4252361/1‑93 (MQ=255)
                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          >  1:808304/1‑93 (MQ=255)
                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          >  1:896010/1‑93 (MQ=255)
                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          <  1:2147668/93‑1 (MQ=255)
                                                 gCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCt                                          <  1:420003/93‑1 (MQ=255)
                                                    gATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtt                                       <  2:2549988/93‑1 (MQ=255)
                                                     atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                      >  1:1972530/1‑93 (MQ=255)
                                                     atatAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGttt                                      <  2:3700610/93‑1 (MQ=255)
                                                      tataGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                     <  1:3778974/93‑1 (MQ=255)
                                                      tataGCTCAGTTGGAAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttt                                     >  2:1535511/1‑93 (MQ=255)
                                                        taGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttt                                   <  1:3249850/93‑1 (MQ=255)
                                                        taGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGtttttt                                   >  2:2766333/1‑93 (MQ=255)
                                                         aGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTc                                  <  1:4362159/93‑1 (MQ=255)
                                                           cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                                >  1:453072/1‑93 (MQ=255)
                                                           cTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCt                                >  2:4111653/1‑93 (MQ=255)
                                                            tCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtt                               <  2:534915/93‑1 (MQ=255)
                                                            tCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtt                               >  1:3354645/1‑93 (MQ=255)
                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  2:295930/93‑1 (MQ=255)
                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  2:1961170/93‑1 (MQ=255)
                                                             cAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTc                              <  1:3769085/93‑1 (MQ=255)
                                                              aGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCt                             >  2:1681035/1‑93 (MQ=255)
                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:3095456/1‑93 (MQ=255)
                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:2713143/1‑93 (MQ=255)
                                                               gTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTg                            >  1:3665499/1‑93 (MQ=255)
                                                               gTTGGCAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCtttgt                            >  1:1006216/1‑90 (MQ=255)
                                                                ttGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGt                           <  1:3335281/93‑1 (MQ=255)
                                                                 tGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGtt                          <  2:806587/93‑1 (MQ=255)
                                                                  tgTAGAGCGCCCCCTTTGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGttt                         <  1:1322014/92‑1 (MQ=255)
                                                                    tAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAt                       <  1:2000361/93‑1 (MQ=255)
                                                                    tAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAt                       <  2:331144/93‑1 (MQ=255)
                                                                     agagCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTAtt                      <  1:2568595/93‑1 (MQ=255)
                                                                       agCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGc                    <  1:1411658/93‑1 (MQ=255)
                                                                         cgcACCTTTGTTAAGGGTGGGGTCGGCAGTTCGAATCTGCGTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAt                  <  2:4420817/93‑1 (MQ=37)
                                                                          gcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAtt                 <  1:767451/93‑1 (MQ=255)
                                                                          gcACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAtt                 >  1:887538/1‑93 (MQ=255)
                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                <  2:1366109/93‑1 (MQ=255)
                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                <  2:1366108/93‑1 (MQ=255)
                                                                           cACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTc                >  1:4096249/1‑93 (MQ=255)
                                                                             cccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaa              >  2:464179/1‑93 (MQ=255)
                                                                              ccTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaac             >  1:40862/1‑93 (MQ=255)
                                                                                ttGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTcaacaa           <  1:1045010/93‑1 (MQ=255)
                                                                                 tGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAg          <  1:1890757/93‑1 (MQ=255)
                                                                                    tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg       >  2:2717651/1‑93 (MQ=255)
                                                                                    tAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCg       <  2:1934490/93‑1 (MQ=255)
                                                                                     aaGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCgg      <  2:518993/93‑1 (MQ=255)
                                                                                     aaGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGGCgg      >  2:1739834/1‑93 (MQ=255)
                                                                                      aGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCggg     >  1:2276278/1‑93 (MQ=255)
                                                                                       aggTGGGGGCGGGAGTTCGAATCTGCCTATCCGCCCCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGc    <  2:4154915/92‑1 (MQ=255)
                                                                                        ggTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCa   <  1:3963573/93‑1 (MQ=255)
                                                                                         gTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAt  <  1:1263712/93‑1 (MQ=255)
                                                                                            |                                                                                         
TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT  >  NC_000913/4173284‑4173465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  >  NC_000913/4173283‑4173468
                                                                                             |                                                                                            
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGG                                                                                              >  SRR4302544.2735915/1‑94 (MQ=60)
 TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGG                                                                                             >  SRR4302544.2134206/1‑94 (MQ=60)
 TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGG                                                                                             <  SRR4302544.3480079/94‑1 (MQ=60)
 TTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGG                                                                                             >  SRR4302544.2171848/1‑94 (MQ=60)
   CCAAGCTGAGGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGT                                                                                           <  SRR4302544.2513029/94‑1 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                          <  SRR4302544.2584964/94‑1 (MQ=60)
    CAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTC                                                                                          >  SRR4302544.4695121/1‑94 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                         >  SRR4302544.489584/1‑94 (MQ=60)
     AAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCG                                                                                         >  SRR4302544.1943200/1‑94 (MQ=60)
      AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGG                                                                                        <  SRR4302544.4401129/94‑1 (MQ=60)
      AGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGG                                                                                        >  SRR4302544.3939222/1‑94 (MQ=60)
       GCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGC                                                                                       >  SRR4302544.4206851/1‑94 (MQ=60)
        CTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCA                                                                                      <  SRR4302544.4501923/94‑1 (MQ=60)
        CTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCA                                                                                      <  SRR4302544.2004296/94‑1 (MQ=60)
          GATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGT                                                                                    >  SRR4302544.2082814/1‑94 (MQ=60)
           ATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTT                                                                                   <  SRR4302544.2636168/94‑1 (MQ=60)
             GATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCG                                                                                 <  SRR4302544.4465571/94‑1 (MQ=60)
             GATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCG                                                                                 <  SRR4302544.2873195/94‑1 (MQ=60)
                 CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATC                                                                             <  SRR4302544.1996230/94‑1 (MQ=60)
                 CGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATC                                                                             >  SRR4302544.3166791/1‑94 (MQ=60)
                  GGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCT                                                                            <  SRR4302544.4203515/94‑1 (MQ=60)
                   GGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTG                                                                           <  SRR4302544.1872612/94‑1 (MQ=60)
                   GGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTG                                                                           <  SRR4302544.3753247/94‑1 (MQ=60)
                     TTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCC                                                                         <  SRR4302544.2077837/94‑1 (MQ=60)
                      TCGATGCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCT                                                                        <  SRR4302544.4305144/94‑1 (MQ=60)
                        GATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGAGAACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTAT                                                                      >  SRR4302544.3037071/1‑94 (MQ=60)
                         ATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATC                                                                     >  SRR4302544.1003988/1‑94 (MQ=60)
                         ATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATC                                                                     >  SRR4302544.2757122/1‑94 (MQ=60)
                         ATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATC                                                                     >  SRR4302544.2757124/1‑94 (MQ=60)
                         ATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATC                                                                     >  SRR4302544.2917312/1‑94 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                    <  SRR4302544.385030/94‑1 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                    <  SRR4302544.3157368/94‑1 (MQ=60)
                          TTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCA                                                                    <  SRR4302544.165939/94‑1 (MQ=60)
                           TCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAG                                                                   >  SRR4302544.3762620/1‑94 (MQ=60)
                           TCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAG                                                                   <  SRR4302544.4456201/94‑1 (MQ=60)
                            CCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGGTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGC                                                                  >  SRR4302544.3132291/1‑94 (MQ=60)
                             CCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCA                                                                 <  SRR4302544.3150069/94‑1 (MQ=60)
                              CGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCAC                                                                <  SRR4302544.783181/94‑1 (MQ=60)
                              CGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCAC                                                                >  SRR4302544.4314467/1‑94 (MQ=60)
                              CGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCAC                                                                >  SRR4302544.3549026/1‑94 (MQ=60)
                                 TGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCAC                                                             <  SRR4302544.2059587/94‑1 (MQ=60)
                                 TGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCAC                                                             <  SRR4302544.3466270/94‑1 (MQ=60)
                                     CGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCT                                                         <  SRR4302544.3590677/94‑1 (MQ=60)
                                      GCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTT                                                        >  SRR4302544.486526/1‑94 (MQ=60)
                                         CCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTC                                                     <  SRR4302544.3767925/94‑1 (MQ=60)
                                         CCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTC                                                     >  SRR4302544.2865698/1‑94 (MQ=60)
                                         CCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTAGAATCTGCCTATCAGCACCACTTCTTTTC                                                     <  SRR4302544.3757525/94‑1 (MQ=60)
                                          CAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCT                                                    >  SRR4302544.432907/1‑94 (MQ=60)
                                           AAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTC                                                   <  SRR4302544.1415945/94‑1 (MQ=60)
                                           AAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTC                                                   >  SRR4302544.4351628/1‑94 (MQ=60)
                                            AGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCC                                                  <  SRR4302544.689487/94‑1 (MQ=60)
                                             GATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCT                                                 <  SRR4302544.3063522/94‑1 (MQ=60)
                                             GATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCT                                                 <  SRR4302544.2193541/94‑1 (MQ=60)
                                                 TGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCT                                             <  SRR4302544.4287722/94‑1 (MQ=60)
                                                 TGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCT                                             >  SRR4302544.816190/1‑94 (MQ=60)
                                                 TGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCT                                             >  SRR4302544.904873/1‑94 (MQ=60)
                                                  GCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG                                            <  SRR4302544.2168125/94‑1 (MQ=60)
                                                  GCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTG                                            <  SRR4302544.424298/94‑1 (MQ=60)
                                                     GATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTT                                         >  SRR4302544.1991700/1‑94 (MQ=60)
                                                       TATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTT                                       <  SRR4302544.3816407/94‑1 (MQ=60)
                                                       TATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTT                                       <  SRR4302544.2914678/94‑1 (MQ=60)
                                                         TAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTC                                     <  SRR4302544.3280945/94‑1 (MQ=60)
                                                         TAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTC                                     <  SRR4302544.3280944/94‑1 (MQ=60)
                                                          AGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCC                                    <  SRR4302544.4406422/94‑1 (MQ=60)
                                                           GCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCT                                   >  SRR4302544.457558/1‑94 (MQ=60)
                                                            CTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTT                                  >  SRR4302544.3386657/1‑94 (MQ=60)
                                                              CAGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCT                                <  SRR4302544.3806458/94‑1 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                               >  SRR4302544.3124934/1‑94 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                               >  SRR4302544.3702052/1‑94 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                               >  SRR4302544.2739245/1‑94 (MQ=60)
                                                               AGTTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTG                               >  SRR4302544.3124933/1‑94 (MQ=60)
                                                               AGTTGGCAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTT‑TGT                              >  SRR4302544.1016220/1‑94 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                             <  SRR4302544.3367052/94‑1 (MQ=60)
                                                                 TTGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTT                             <  SRR4302544.1353758/94‑1 (MQ=60)
                                                                  TGGTAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTT                            <  SRR4302544.1154831/94‑1 (MQ=60)
                                                                   TGTAGAGCGCCCCCTTTGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTA                           <  SRR4302544.1335021/94‑1 (MQ=60)
                                                                     TAGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATT                         <  SRR4302544.2019783/94‑1 (MQ=60)
                                                                      AGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTG                        <  SRR4302544.2593272/94‑1 (MQ=60)
                                                                      AGAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTG                        >  SRR4302544.4136801/1‑94 (MQ=60)
                                                                       GAGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGC                       <  SRR4302544.851049/94‑1 (MQ=60)
                                                                        AGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCA                      >  SRR4302544.617637/1‑94 (MQ=60)
                                                                        AGCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCA                      <  SRR4302544.1425714/94‑1 (MQ=60)
                                                                         GCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAT                     >  SRR4302544.3649021/1‑94 (MQ=60)
                                                                         GCGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCAT                     >  SRR4302544.3367842/1‑94 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                    >  SRR4302544.3727908/1‑94 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                    >  SRR4302544.896334/1‑94 (MQ=60)
                                                                          CGCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATT                    >  SRR4302544.727689/1‑94 (MQ=60)
                                                                           GCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTC                   >  SRR4302544.4137014/1‑94 (MQ=60)
                                                                           GCACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTC                   <  SRR4302544.774923/94‑1 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                  <  SRR4302544.3106676/94‑1 (MQ=60)
                                                                            CACCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCA                  <  SRR4302544.3309920/94‑1 (MQ=60)
                                                                              CCCTTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAAC                >  SRR4302544.41268/1‑94 (MQ=60)
                                                                                 TTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAG             <  SRR4302544.1055372/94‑1 (MQ=60)
                                                                                 TTGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTCCCTTCTGTTTATTGCATTCAACAAG             <  SRR4302544.3993993/94‑1 (MQ=60)
                                                                                  TGGTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGT            <  SRR4302544.1909022/94‑1 (MQ=60)
                                                                                    GTAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCG          <  SRR4302544.4105479/94‑1 (MQ=60)
                                                                                     TAAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGG         <  SRR4302544.1318025/94‑1 (MQ=60)
                                                                                      AAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGG        <  SRR4302544.147441/94‑1 (MQ=60)
                                                                                      AAGGGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGG        >  SRR4302544.2297711/1‑94 (MQ=60)
                                                                                         GGTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCAT     <  SRR4302544.4002995/94‑1 (MQ=60)
                                                                                          GTGGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATG    <  SRR4302544.1276147/94‑1 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  <  SRR4302544.1347289/94‑1 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  <  SRR4302544.1562324/94‑1 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  >  SRR4302544.2731166/1‑94 (MQ=60)
                                                                                            GGGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  <  SRR4302544.4222709/94‑1 (MQ=60)
                                                                                             |                                                                                            
CTTCCAAGCTGATGATGCGGGTTCGATTCCCGCTGCCCGCTCCAAGATGTGCTGATATAGCTCAGTTGGTAGAGCGCACCCTTGGTAAGGGTGAGGTCGGCAGTTCGAATCTGCCTATCAGCACCACTTCTTTTCTCCTCCCTGTTTTTTCCTTCTGTTTATTGCATTCAACAAGTCGGGCATGTT  >  NC_000913/4173283‑4173468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑