Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,853 G→A V178I (GTT→ATT)  nusG → NusG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,8530GA96.4% 573.0 / 3.4 167V178I (GTT→ATT) nusGNusG
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base A (77/84);  total (79/88)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 174 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942
                                                                                          |                                                                                         
gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                         <  2:1295030/93‑1 (MQ=255)
  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       <  1:3289670/93‑1 (MQ=255)
   gaagaaGTGGATTACGTGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCGGGACTTCAGCCAGAATGaa                                                                                      >  2:4217630/1‑93 (MQ=255)
   gaagaaGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCCGGTTGaa                                                                                      >  2:3106316/1‑93 (MQ=255)
   gaagaaGTGGATTACGAGAAATCTCGTATGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaa                                                                                      >  2:705242/1‑93 (MQ=255)
     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    >  1:3344842/1‑93 (MQ=255)
     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    <  1:3716363/93‑1 (MQ=255)
      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   >  1:4473832/1‑93 (MQ=255)
      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   >  2:4462176/1‑93 (MQ=255)
      aaagTGGAGTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  2:3348474/92‑1 (MQ=255)
        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 <  1:3389952/93‑1 (MQ=255)
           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              <  2:2689509/93‑1 (MQ=255)
               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                          <  1:4747831/93‑1 (MQ=255)
               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                          <  2:1937469/93‑1 (MQ=255)
               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                          <  1:1234601/93‑1 (MQ=255)
                 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAg                                                                        <  1:3894961/93‑1 (MQ=255)
                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGc                                                                       <  1:4367517/93‑1 (MQ=255)
                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  2:1802941/93‑1 (MQ=255)
                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  1:2547111/1‑93 (MQ=255)
                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     >  1:1052689/1‑93 (MQ=255)
                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   <  1:2251158/93‑1 (MQ=255)
                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   <  2:1332347/93‑1 (MQ=255)
                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   >  1:1525996/1‑93 (MQ=255)
                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCa                                                                  <  1:2364395/93‑1 (MQ=255)
                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCa                                                                  >  1:4258111/1‑93 (MQ=255)
                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCa                                                                  <  2:3154558/93‑1 (MQ=255)
                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 >  2:2769331/1‑93 (MQ=255)
                         ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaa                                                                >  1:52830/1‑93 (MQ=255)
                          tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCGGCCAGATTGAAAAAGCCTACCCCAGAGATAaaaa                                                               >  1:2225953/1‑93 (MQ=255)
                           cGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaa                                                              <  2:3585899/93‑1 (MQ=255)
                           cGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaa                                                              <  1:1174966/93‑1 (MQ=255)
                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  1:4589607/1‑93 (MQ=255)
                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             <  1:4615781/93‑1 (MQ=255)
                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  1:4347878/1‑93 (MQ=255)
                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGCTCAAAAAAgcg                                                          >  1:174153/1‑93 (MQ=255)
                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  1:3540998/1‑93 (MQ=255)
                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          <  2:1182446/93‑1 (MQ=255)
                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  2:308081/1‑93 (MQ=255)
                               tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcg                                                          >  1:2813078/1‑93 (MQ=255)
                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                         >  2:635922/1‑93 (MQ=255)
                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                         >  1:2803197/1‑93 (MQ=255)
                                   aGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcgt                                                      >  1:4045538/1‑92 (MQ=255)
                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     <  2:3290958/93‑1 (MQ=255)
                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     >  2:45520/1‑93 (MQ=255)
                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTACCCCGGCGATCAAAAAAGCGGCGAtt                                                    <  2:2365388/93‑1 (MQ=255)
                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    >  1:901893/1‑93 (MQ=255)
                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  2:3241682/93‑1 (MQ=255)
                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    <  2:2730153/93‑1 (MQ=255)
                                     tgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAtt                                                    >  2:4257740/1‑93 (MQ=255)
                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                >  1:4606481/1‑93 (MQ=255)
                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  1:1581937/93‑1 (MQ=255)
                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  2:596540/93‑1 (MQ=255)
                                         tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                <  2:1406288/93‑1 (MQ=255)
                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                              >  2:340787/1‑93 (MQ=255)
                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             <  1:2084599/93‑1 (MQ=255)
                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             <  1:3477151/93‑1 (MQ=255)
                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACCTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             <  1:3763172/93‑1 (MQ=255)
                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  2:3463881/1‑93 (MQ=255)
                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            <  1:4633388/93‑1 (MQ=255)
                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:956664/1‑93 (MQ=255)
                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:1055061/1‑93 (MQ=255)
                                               tATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGc                                          >  1:587067/1‑93 (MQ=255)
                                                aTCTTCGGTCGTGTGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         <  2:2068321/93‑1 (MQ=255)
                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         >  2:2634589/1‑93 (MQ=255)
                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        >  2:1558194/1‑93 (MQ=255)
                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       <  2:351884/93‑1 (MQ=255)
                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcacag                                      >  2:1736888/1‑92 (MQ=255)
                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  1:987005/93‑1 (MQ=255)
                                                    tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                     <  2:1492866/93‑1 (MQ=255)
                                                     cGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                    >  1:2302351/1‑93 (MQ=255)
                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  2:4415866/93‑1 (MQ=255)
                                                       gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                  >  1:104958/1‑93 (MQ=255)
                                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                <  1:1618223/93‑1 (MQ=255)
                                                          gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:1780810/93‑1 (MQ=255)
                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              <  2:2963855/93‑1 (MQ=255)
                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  2:4153381/1‑93 (MQ=255)
                                                           tGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                              >  1:4021015/1‑93 (MQ=255)
                                                            gCGACCCCGGTAGAGCTGGACTTCAGCCATATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                             <  2:1009381/93‑1 (MQ=255)
                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           <  2:2322328/93‑1 (MQ=255)
                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           >  2:1057377/1‑93 (MQ=255)
                                                               aCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                          <  1:4212866/93‑1 (MQ=255)
                                                                ccccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGACTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTgg                         <  1:2142538/93‑1 (MQ=255)
                                                                   cGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAt                      <  1:1972592/93‑1 (MQ=255)
                                                                    ggTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                     <  2:2501745/93‑1 (MQ=255)
                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 >  2:1585841/1‑93 (MQ=255)
                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              <  2:3072076/93‑1 (MQ=255)
                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              >  1:3221138/1‑93 (MQ=255)
                                                                            tGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtttag             >  2:3015411/1‑91 (MQ=255)
                                                                            tGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg             >  2:2122940/1‑93 (MQ=255)
                                                                             ggACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc            <  1:4431368/93‑1 (MQ=255)
                                                                               aCTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCgcgc          <  1:3170661/93‑1 (MQ=255)
                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       <  2:1207048/93‑1 (MQ=255)
                                                                                  tCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtt       >  2:859756/1‑93 (MQ=255)
                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  2:4578276/93‑1 (MQ=255)
                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCCGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtttt     <  2:4050181/93‑1 (MQ=255)
                                                                                     gCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg    <  2:3025112/93‑1 (MQ=255)
                                                                                      ccAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt   >  2:3247225/1‑93 (MQ=255)
                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  >  1:3430599/1‑93 (MQ=255)
                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  >  1:3430600/1‑93 (MQ=255)
                                                                                       cAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt  <  1:1400270/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  NC_000913/4175760‑4175946
                                                                                             |                                                                                             
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGA                                                                                               >  SRR4302544.3914180/1‑94 (MQ=60)
   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTCGACTTCAGCCAGATTG                                                                                            >  SRR4302544.3387369/1‑94 (MQ=60)
     TGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAA                                                                                          <  SRR4302544.3321015/94‑1 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                        <  SRR4302544.168773/94‑1 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                        >  SRR4302544.3376744/1‑94 (MQ=60)
        AGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAA                                                                                       <  SRR4302544.3753330/94‑1 (MQ=60)
        AGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAA                                                                                       >  SRR4302544.4518826/1‑94 (MQ=60)
           AGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCC                                                                                    <  SRR4302544.3422349/94‑1 (MQ=60)
            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                   <  SRR4302544.3442337/94‑1 (MQ=60)
              GGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAA                                                                                 >  SRR4302544.1284928/1‑94 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                             <  SRR4302544.1246811/94‑1 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                             <  SRR4302544.4400680/94‑1 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                             <  SRR4302544.4794599/94‑1 (MQ=60)
                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGC                                                                           <  SRR4302544.3933652/94‑1 (MQ=60)
                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                           >  SRR4302544.2571568/1‑94 (MQ=60)
                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCG                                                                          <  SRR4302544.4411812/94‑1 (MQ=60)
                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGA                                                                         >  SRR4302544.1063130/1‑94 (MQ=60)
                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGA                                                                         >  SRR4302544.1063132/1‑94 (MQ=60)
                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                       >  SRR4302544.1541147/1‑94 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                      >  SRR4302544.4301526/1‑94 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                      <  SRR4302544.2272315/94‑1 (MQ=60)
                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAA                                                                     <  SRR4302544.2386776/94‑1 (MQ=60)
                           TCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAA                                                                    >  SRR4302544.53368/1‑94 (MQ=60)
                            CTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCGGCCAGATTGAAAAAGCCTACCCCAGAGATAAAAA                                                                   >  SRR4302544.2246894/1‑94 (MQ=60)
                            CTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAA                                                                   >  SRR4302544.2246895/1‑94 (MQ=60)
                              CGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                                 >  SRR4302544.4392071/1‑94 (MQ=60)
                              CGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                                 >  SRR4302544.4635283/1‑94 (MQ=60)
                              CGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                                 <  SRR4302544.1186682/94‑1 (MQ=60)
                               GTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAG                                                                <  SRR4302544.4661605/94‑1 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGCTCAAAAAAGCG                                                              >  SRR4302544.175870/1‑94 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              >  SRR4302544.3574890/1‑94 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              >  SRR4302544.2840226/1‑94 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                              <  SRR4302544.1967885/94‑1 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                             >  SRR4302544.467295/1‑94 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                             <  SRR4302544.981034/94‑1 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                             >  SRR4302544.2830219/1‑94 (MQ=60)
                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                            >  SRR4302544.4760931/1‑94 (MQ=60)
                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                           >  SRR4302544.605549/1‑94 (MQ=60)
                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                           <  SRR4302544.4510473/94‑1 (MQ=60)
                                     AAGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGT                                                          >  SRR4302544.4085837/1‑94 (MQ=60)
                                       GTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATT                                                        >  SRR4302544.910817/1‑94 (MQ=60)
                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                       >  SRR4302544.3532663/1‑94 (MQ=60)
                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                    >  SRR4302544.4652246/1‑94 (MQ=60)
                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                   <  SRR4302544.1597570/94‑1 (MQ=60)
                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                                  <  SRR4302544.955716/94‑1 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                >  SRR4302544.966068/1‑94 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                <  SRR4302544.3510305/94‑1 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                <  SRR4302544.2104649/94‑1 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                >  SRR4302544.1065522/1‑94 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACCTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                                <  SRR4302544.3800514/94‑1 (MQ=60)
                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                               <  SRR4302544.3186049/94‑1 (MQ=60)
                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                               <  SRR4302544.4679315/94‑1 (MQ=60)
                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                              >  SRR4302544.592825/1‑94 (MQ=60)
                                                  CATCTTCGTTCGTCCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGACAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                             <  SRR4302544.1780162/94‑1 (MQ=60)
                                                     CTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                          >  SRR4302544.1488705/1‑94 (MQ=60)
                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         >  SRR4302544.3946274/1‑94 (MQ=60)
                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                         <  SRR4302544.996688/94‑1 (MQ=60)
                                                       TCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                        >  SRR4302544.2324094/1‑94 (MQ=60)
                                                       TCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                        <  SRR4302544.2181940/94‑1 (MQ=60)
                                                        CGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGC                                       <  SRR4302544.42901/94‑1 (MQ=60)
                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                      <  SRR4302544.4554504/94‑1 (MQ=60)
                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                      >  SRR4302544.105922/1‑94 (MQ=60)
                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                      <  SRR4302544.487302/94‑1 (MQ=60)
                                                            CGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGA                                   >  SRR4302544.4062707/1‑94 (MQ=60)
                                                            CGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGA                                   <  SRR4302544.1634170/94‑1 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                  <  SRR4302544.1576770/94‑1 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                  >  SRR4302544.4061037/1‑94 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                                  <  SRR4302544.1798230/94‑1 (MQ=60)
                                                                 GACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTG                              >  SRR4302544.1172337/1‑94 (MQ=60)
                                                                  ACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGG                             <  SRR4302544.4255320/94‑1 (MQ=60)
                                                                   CCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGA                            <  SRR4302544.3695479/94‑1 (MQ=60)
                                                                   CCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGA                            >  SRR4302544.3210544/1‑94 (MQ=60)
                                                                   CCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGACTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGA                            <  SRR4302544.2162980/94‑1 (MQ=60)
                                                                     CCGGTAGAGCTGGACGTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                          <  SRR4302544.3869211/94‑1 (MQ=60)
                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                         <  SRR4302544.1991762/94‑1 (MQ=60)
                                                                       GGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAC                        >  SRR4302544.4648744/1‑94 (MQ=60)
                                                                        GTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACA                       <  SRR4302544.2380359/94‑1 (MQ=60)
                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                     >  SRR4302544.3507370/1‑94 (MQ=60)
                                                                             GCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTC                  >  SRR4302544.3251885/1‑94 (MQ=60)
                                                                              GTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCG                 <  SRR4302544.2740832/94‑1 (MQ=60)
                                                                              CTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCG                 >  SRR4302544.4337789/1‑94 (MQ=60)
                                                                               TGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGC                >  SRR4302544.2391931/1‑94 (MQ=60)
                                                                                GGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCG               <  SRR4302544.4476075/94‑1 (MQ=60)
                                                                                  ACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCC             <  SRR4302544.3200836/94‑1 (MQ=60)
                                                                                   CTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCT            <  SRR4302544.3255658/94‑1 (MQ=60)
                                                                                       AGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG        >  SRR4302544.2142928/1‑94 (MQ=60)
                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT      >  SRR4302544.3463387/1‑94 (MQ=60)
                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT      >  SRR4302544.3463388/1‑94 (MQ=60)
                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT     <  SRR4302544.1414219/94‑1 (MQ=60)
                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT    >  SRR4302544.2429764/1‑94 (MQ=60)
                                                                                           AGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTT    >  SRR4302544.2821235/1‑94 (MQ=60)
                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT   <  SRR4302544.3204656/94‑1 (MQ=60)
                                                                                            GATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTT   <  SRR4302544.3430792/94‑1 (MQ=60)
                                                                                             ATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  SRR4302544.3428100/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTA  >  NC_000913/4175760‑4175946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑