Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,175,853 G→A V178I (GTT→ATT)  nusG → NusG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,175,8530GA99.7% 1066.9 / ‑5.7 285V178I (GTT→ATT) nusGNusG
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (133/151);  total (133/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 292 total aligned reads displayed.

GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942
                                                                                          |                                                                                         
gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                         <  1:6072415/93‑1 (MQ=255)
gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                         <  1:2093718/93‑1 (MQ=255)
gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAtt                                                                                         >  2:535461/1‑93 (MQ=255)
 ttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTg                                                                                        >  1:3067386/1‑93 (MQ=255)
  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       >  2:3855743/1‑93 (MQ=255)
  tgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGa                                                                                       >  1:2571561/1‑93 (MQ=255)
   gaagaaGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaa                                                                                      <  1:4702275/93‑1 (MQ=255)
   gaagaaGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaa                                                                                      <  2:2904310/93‑1 (MQ=255)
    aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaa                                                                                     <  2:5253178/93‑1 (MQ=255)
    aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaa                                                                                     >  2:5784393/1‑93 (MQ=255)
     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    >  2:4920269/1‑93 (MQ=255)
     agaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaa                                                                                    >  1:5706136/1‑93 (MQ=255)
      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  1:5287783/93‑1 (MQ=255)
      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   >  1:3606382/1‑93 (MQ=255)
      gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGaaaaa                                                                                   <  1:3522471/93‑1 (MQ=255)
        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCCGATTGAAAAAGc                                                                                 >  1:3679613/1‑93 (MQ=255)
        agTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGc                                                                                 >  2:6608365/1‑93 (MQ=255)
         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                >  2:3961982/1‑93 (MQ=255)
         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                <  2:3634160/93‑1 (MQ=255)
         gTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGcc                                                                                <  1:4879155/93‑1 (MQ=255)
           ggATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTa                                                                              >  1:1491139/1‑93 (MQ=255)
               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                          <  1:1125171/93‑1 (MQ=255)
               tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAccc                                                                          <  2:3034725/93‑1 (MQ=255)
                aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCa                                                                         <  1:1603349/93‑1 (MQ=255)
                 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                        <  1:3319573/93‑1 (MQ=255)
                 cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAg                                                                        <  2:4285849/93‑1 (MQ=255)
                  gagaAATCTTGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:4014830/93‑1 (MQ=255)
                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:3963778/93‑1 (MQ=255)
                  gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       >  1:4339586/1‑93 (MQ=255)
                  gacaaaTCTCGTCTGAAAGTGTCTGTTTCTATCTCCGGTCGGGCGCCGCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGc                                                                       <  1:2326319/90‑1 (MQ=255)
                   agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCg                                                                      >  1:3813334/1‑93 (MQ=255)
                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     >  2:1928240/1‑93 (MQ=255)
                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     <  2:3417325/93‑1 (MQ=255)
                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     <  2:5855033/93‑1 (MQ=255)
                    gaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGa                                                                     >  1:2412481/1‑93 (MQ=255)
                     aaaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAt                                                                    <  2:4688833/93‑1 (MQ=255)
                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   <  2:6333418/93‑1 (MQ=255)
                      aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATc                                                                   <  1:3691298/93‑1 (MQ=255)
                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCa                                                                  >  1:533355/1‑93 (MQ=255)
                       aTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCa                                                                  >  1:3486964/1‑93 (MQ=255)
                        tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaa                                                                 <  1:1531009/93‑1 (MQ=255)
                          tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaa                                                               >  1:4495938/1‑93 (MQ=255)
                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             >  1:2613148/1‑93 (MQ=255)
                            gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCaaaaaa                                                             <  2:2520452/93‑1 (MQ=255)
                             tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                            >  2:4548783/1‑93 (MQ=255)
                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                         >  1:1855489/1‑93 (MQ=255)
                                gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                         >  1:4110102/1‑93 (MQ=255)
                                 aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                        <  1:5022536/93‑1 (MQ=255)
                                  aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggcg                                                       >  1:989453/1‑93 (MQ=255)
                                    gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAt                                                     <  2:1709238/93‑1 (MQ=255)
                                      gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAttt                                                   >  1:3240862/1‑93 (MQ=255)
                                       tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTa                                                  >  1:1946718/1‑93 (MQ=255)
                                        cTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTaa                                                 <  2:1062518/93‑1 (MQ=255)
                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               >  1:1021322/1‑93 (MQ=255)
                                          gTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATc                                               <  1:5948436/93‑1 (MQ=255)
                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                              >  1:4249021/1‑93 (MQ=255)
                                           tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCg                                              <  2:3538778/93‑1 (MQ=255)
                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             >  1:6477771/1‑93 (MQ=255)
                                            ttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGt                                             >  1:63822/1‑93 (MQ=255)
                                             tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                            >  1:1562103/1‑93 (MQ=255)
                                              cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                           <  1:1321545/93‑1 (MQ=255)
                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         >  2:1335051/1‑93 (MQ=255)
                                                aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGca                                         >  2:4616244/1‑93 (MQ=255)
                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        <  2:4170502/93‑1 (MQ=255)
                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        <  1:2920085/93‑1 (MQ=255)
                                                 tCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                        >  2:2557110/1‑93 (MQ=255)
                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       >  2:78143/1‑93 (MQ=255)
                                                  cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcaca                                       >  1:581570/1‑93 (MQ=255)
                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGTCTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  1:4412656/93‑1 (MQ=255)
                                                   ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                      <  2:1504999/93‑1 (MQ=255)
                                                    tCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                     <  1:4764919/93‑1 (MQ=255)
                                                    tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                     <  2:5392678/93‑1 (MQ=255)
                                                      ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGc                                   <  1:2271010/93‑1 (MQ=255)
                                                         cGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                >  2:278269/1‑93 (MQ=255)
                                                          gTGCGACCGCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                               <  1:2829006/93‑1 (MQ=255)
                                                            gCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgaga                             <  2:5579188/93‑1 (MQ=255)
                                                             cGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                            <  1:6167540/93‑1 (MQ=255)
                                                              gACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAATCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                           <  2:3186821/93‑1 (MQ=255)
                                                                 cccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                        >  2:1607923/1‑93 (MQ=255)
                                                                  ccGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                       <  2:247695/93‑1 (MQ=255)
                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    >  1:3042499/1‑93 (MQ=255)
                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    >  2:2361964/1‑93 (MQ=255)
                                                                     gTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                    >  2:6493691/1‑93 (MQ=255)
                                                                      tAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACa                   <  2:2049423/93‑1 (MQ=255)
                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  <  1:5195514/93‑1 (MQ=255)
                                                                       agagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                  <  1:4631060/93‑1 (MQ=255)
                                                                        gagCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                 <  1:6543016/93‑1 (MQ=255)
                                                                         agCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                >  2:270531/1‑93 (MQ=255)
                                                                           cTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc              <  2:5086858/93‑1 (MQ=255)
                                                                            tGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg             <  2:4076194/93‑1 (MQ=255)
                                                                             ggACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgc            <  1:5578349/93‑1 (MQ=255)
                                                                                cTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGcc         <  2:819288/93‑1 (MQ=255)
                                                                                cTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGcc         >  1:1709751/1‑93 (MQ=255)
                                                                                 ttCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCt        >  2:6022576/1‑93 (MQ=255)
                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  2:882286/93‑1 (MQ=255)
                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  1:6645556/93‑1 (MQ=255)
                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  1:869270/93‑1 (MQ=255)
                                                                                   cAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt      <  2:1258587/93‑1 (MQ=255)
                                                                                    aGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCtttt     <  2:6109266/93‑1 (MQ=255)
                                                                                     gCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg    >  2:4205167/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT  >  NC_000913/4175763‑4175942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT  >  NC_000913/4175760‑4175943
                                                                                             |                                                                                          
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGA                                                                                            >  SRR4302536.4839446/1‑94 (MQ=60)
 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                           >  SRR4302536.2415406/1‑94 (MQ=60)
 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                           <  SRR4302536.1061568/94‑1 (MQ=60)
 TTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                           <  SRR4302536.1950118/94‑1 (MQ=60)
 TTGTTGAAGAAGTGGATTACGACAAATCTCGTCTGAAAGTGTCTGTTTCTATTTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAT                                                                                           <  SRR4302536.5522241/94‑1 (MQ=60)
  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                          <  SRR4302536.3562091/94‑1 (MQ=60)
  TGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATT                                                                                          >  SRR4302536.3649815/1‑94 (MQ=60)
   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTG                                                                                         >  SRR4302536.3157299/1‑94 (MQ=60)
   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTG                                                                                         <  SRR4302536.2156715/94‑1 (MQ=60)
   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTG                                                                                         <  SRR4302536.1645026/94‑1 (MQ=60)
   GTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTG                                                                                         <  SRR4302536.6253561/94‑1 (MQ=60)
    TTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGA                                                                                        <  SRR4302536.4104217/94‑1 (MQ=60)
    TTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGA                                                                                        >  SRR4302536.2648893/1‑94 (MQ=60)
      GAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAA                                                                                      <  SRR4302536.4843666/94‑1 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                     >  SRR4302536.5115830/1‑94 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                     >  SRR4302536.3212135/1‑94 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                     >  SRR4302536.1987466/1‑94 (MQ=60)
       AAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                     >  SRR4302536.5875821/1‑94 (MQ=60)
       AAGAAGCGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAA                                                                                     >  SRR4302536.6172523/1‑94 (MQ=60)
        AGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAA                                                                                    >  SRR4302536.3714224/1‑94 (MQ=60)
         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                   <  SRR4302536.5445431/94‑1 (MQ=60)
         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                   <  SRR4302536.3627723/94‑1 (MQ=60)
         GAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                   >  SRR4302536.6051928/1‑94 (MQ=60)
         GAAGTGGATTAAGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAG                                                                                   <  SRR4302536.159378/94‑1 (MQ=60)
          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCCGATTGAAAAAGC                                                                                  >  SRR4302536.3789601/1‑94 (MQ=60)
          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGC                                                                                  >  SRR4302536.6193385/1‑94 (MQ=60)
          AAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGAGTGAAAAAGC                                                                                  <  SRR4302536.6750856/94‑1 (MQ=60)
            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                <  SRR4302536.5025954/94‑1 (MQ=60)
            GTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCT                                                                                >  SRR4302536.4065987/1‑94 (MQ=60)
             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                               >  SRR4302536.1535187/1‑94 (MQ=60)
             TGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTA                                                                               >  SRR4302536.3530404/1‑94 (MQ=60)
               GATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAAC                                                                             <  SRR4302536.3718876/94‑1 (MQ=60)
                ATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACC                                                                            <  SRR4302536.2862492/94‑1 (MQ=60)
                ATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACC                                                                            <  SRR4302536.6648187/94‑1 (MQ=60)
                ATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACC                                                                            >  SRR4302536.4148619/1‑94 (MQ=60)
                 TTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCC                                                                           >  SRR4302536.541126/1‑94 (MQ=60)
                  TGCGAGACATCTCGTCTGACAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                          <  SRR4302536.6923710/94‑1 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGGCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                          <  SRR4302536.1158971/94‑1 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                          >  SRR4302536.4152401/1‑94 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                          <  SRR4302536.5349299/94‑1 (MQ=60)
                  TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCA                                                                          >  SRR4302536.1831293/1‑94 (MQ=60)
                   ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAG                                                                         <  SRR4302536.1650136/94‑1 (MQ=60)
                   ACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAG                                                                         >  SRR4302536.4124859/1‑94 (MQ=60)
                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                        <  SRR4302536.3417701/94‑1 (MQ=60)
                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                        >  SRR4302536.4469552/1‑94 (MQ=60)
                    CGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGC                                                                        <  SRR4302536.4559910/94‑1 (MQ=60)
                     GAGAAATCTTGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       <  SRR4302536.4133953/94‑1 (MQ=60)
                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       >  SRR4302536.1560604/1‑94 (MQ=60)
                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       <  SRR4302536.4081446/94‑1 (MQ=60)
                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       >  SRR4302536.3305931/1‑94 (MQ=60)
                     GAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       >  SRR4302536.3927002/1‑94 (MQ=60)
                     GACAAATCTCGTCTGAAAGTGTCTGTTTCTATCTCCGGTCGGGCGCCGCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCG                                                                       <  SRR4302536.2396926/94‑1 (MQ=60)
                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGT                                                                      >  SRR4302536.6931431/1‑94 (MQ=60)
                      AGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGA                                                                      >  SRR4302536.2485458/1‑94 (MQ=60)
                       GAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAT                                                                     <  SRR4302536.269823/94‑1 (MQ=60)
                       GAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGAT                                                                     <  SRR4302536.4067183/94‑1 (MQ=60)
                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                    <  SRR4302536.5967940/94‑1 (MQ=60)
                        AAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATC                                                                    <  SRR4302536.5898937/94‑1 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                   >  SRR4302536.3590969/1‑94 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                   >  SRR4302536.4421787/1‑94 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                   <  SRR4302536.3801699/94‑1 (MQ=60)
                         AATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCA                                                                   >  SRR4302536.546985/1‑94 (MQ=60)
                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                  <  SRR4302536.1543836/94‑1 (MQ=60)
                          ATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAA                                                                  <  SRR4302536.346898/94‑1 (MQ=60)
                           TCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAA                                                                 <  SRR4302536.1576056/94‑1 (MQ=60)
                            CTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAA                                                                >  SRR4302536.1111064/1‑94 (MQ=60)
                            CTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAA                                                                >  SRR4302536.4630894/1‑94 (MQ=60)
                            CTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAA                                                                >  SRR4302536.3075280/1‑94 (MQ=60)
                              CGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAA                                                              >  SRR4302536.2691559/1‑94 (MQ=60)
                               CTCTGAAAGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAG                                                             <  SRR4302536.5679741/94‑1 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                           >  SRR4302536.2656078/1‑94 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                           >  SRR4302536.6792861/1‑94 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                           <  SRR4302536.1022106/94‑1 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                           <  SRR4302536.1865566/94‑1 (MQ=60)
                                 CTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCG                                                           >  SRR4302536.3880922/1‑94 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                          >  SRR4302536.1911395/1‑94 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                          >  SRR4302536.5870225/1‑94 (MQ=60)
                                  TGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGG                                                          >  SRR4302536.4232035/1‑94 (MQ=60)
                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                         >  SRR4302536.6516418/1‑94 (MQ=60)
                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                         <  SRR4302536.2647047/94‑1 (MQ=60)
                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                         <  SRR4302536.427340/94‑1 (MQ=60)
                                   GAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGC                                                         >  SRR4302536.4608397/1‑94 (MQ=60)
                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                        >  SRR4302536.6535893/1‑94 (MQ=60)
                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                        <  SRR4302536.5173078/94‑1 (MQ=60)
                                    AAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCG                                                        >  SRR4302536.1018644/1‑94 (MQ=60)
                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                      >  SRR4302536.3367293/1‑94 (MQ=60)
                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                      >  SRR4302536.6620364/1‑94 (MQ=60)
                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                      >  SRR4302536.542985/1‑94 (MQ=60)
                                      AGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGAT                                                      >  SRR4302536.3844655/1‑94 (MQ=60)
                                        TGTCTGTTTTTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                    <  SRR4302536.2568871/94‑1 (MQ=60)
                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                    >  SRR4302536.6011468/1‑94 (MQ=60)
                                        TGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTT                                                    >  SRR4302536.3335854/1‑94 (MQ=60)
                                         GTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTA                                                   >  SRR4302536.2005263/1‑94 (MQ=60)
                                         GTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTA                                                   >  SRR4302536.1933105/1‑94 (MQ=60)
                                          TCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAA                                                  <  SRR4302536.4812082/94‑1 (MQ=60)
                                          TCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAA                                                  <  SRR4302536.4510620/94‑1 (MQ=60)
                                          TCTGTTTCTATCTTCGGGCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCCAAACCAGCGATCAACAAAGCGGCGATTTAA                                                  >  SRR4302536.6017714/1‑94 (MQ=60)
                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                 <  SRR4302536.3133234/94‑1 (MQ=60)
                                           CTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAT                                                 >  SRR4302536.4901010/1‑94 (MQ=60)
                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                <  SRR4302536.5172830/94‑1 (MQ=60)
                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                <  SRR4302536.633193/94‑1 (MQ=60)
                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                <  SRR4302536.2932170/94‑1 (MQ=60)
                                            TGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATC                                                >  SRR4302536.1051555/1‑94 (MQ=60)
                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                               >  SRR4302536.4375585/1‑94 (MQ=60)
                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                               >  SRR4302536.2679990/1‑94 (MQ=60)
                                             GTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCG                                               <  SRR4302536.6125646/94‑1 (MQ=60)
                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                              >  SRR4302536.5780154/1‑94 (MQ=60)
                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                              >  SRR4302536.65570/1‑94 (MQ=60)
                                              TTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGT                                              >  SRR4302536.6671045/1‑94 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                             <  SRR4302536.3597085/94‑1 (MQ=60)
                                               TTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTT                                             >  SRR4302536.1607978/1‑94 (MQ=60)
                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGTGATCAAAAAAGCGGCGATTTAATCGTTG                                            <  SRR4302536.5352583/94‑1 (MQ=60)
                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                            <  SRR4302536.2364528/94‑1 (MQ=60)
                                                TCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTG                                            <  SRR4302536.4672336/94‑1 (MQ=60)
                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                           <  SRR4302536.1360996/94‑1 (MQ=60)
                                                 CTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGC                                           <  SRR4302536.2261525/94‑1 (MQ=60)
                                                  TATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCA                                          >  SRR4302536.4427167/1‑94 (MQ=60)
                                                   ATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCAA                                         >  SRR4302536.4931283/1‑94 (MQ=60)
                                                    TCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACA                                        <  SRR4302536.3006263/94‑1 (MQ=60)
                                                    TCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACA                                        >  SRR4302536.596342/1‑94 (MQ=60)
                                                    TCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACA                                        >  SRR4302536.1007652/1‑94 (MQ=60)
                                                     CTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAA                                       >  SRR4302536.1426432/1‑94 (MQ=60)
                                                      TTCGGTCGTGCGACCCCGGTAGAGCTGGTCTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAG                                      <  SRR4302536.4544702/94‑1 (MQ=60)
                                                       TCGGTCGTGCGCCCCTGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAGCCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                     <  SRR4302536.2192610/94‑1 (MQ=60)
                                                       TCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                     <  SRR4302536.4908417/94‑1 (MQ=60)
                                                       TCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                     >  SRR4302536.3625278/1‑94 (MQ=60)
                                                       TCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGG                                     <  SRR4302536.3753727/94‑1 (MQ=60)
                                                        CGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGC                                    <  SRR4302536.3635679/94‑1 (MQ=60)
                                                         GGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCG                                   <  SRR4302536.2339808/94‑1 (MQ=60)
                                                          GTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGT                                  >  SRR4302536.5469539/1‑94 (MQ=60)
                                                          GTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGA                                  >  SRR4302536.1742165/1‑94 (MQ=60)
                                                            CGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGA                                >  SRR4302536.567502/1‑94 (MQ=60)
                                                             GTGCGACCGCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                               <  SRR4302536.2912765/94‑1 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                               >  SRR4302536.1291015/1‑94 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                               <  SRR4302536.961519/94‑1 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                               >  SRR4302536.1423824/1‑94 (MQ=60)
                                                             GTGCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAG                               >  SRR4302536.475499/1‑94 (MQ=60)
                                                               GCGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAT                             <  SRR4302536.2971290/94‑1 (MQ=60)
                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                            <  SRR4302536.2115544/94‑1 (MQ=60)
                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                            <  SRR4302536.6351249/94‑1 (MQ=60)
                                                                CGACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATT                            <  SRR4302536.4885567/94‑1 (MQ=60)
                                                                 GACCCCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTG                           <  SRR4302536.6263328/94‑1 (MQ=60)
                                                                   CCCTGGTAGAGCTGGCCTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGA                         <  SRR4302536.2076638/94‑1 (MQ=60)
                                                                     CCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                       <  SRR4302536.4635198/94‑1 (MQ=60)
                                                                     CCGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAAT                       <  SRR4302536.3062461/94‑1 (MQ=60)
                                                                      CGGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATA                      <  SRR4302536.247135/94‑1 (MQ=60)
                                                                       GGTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAC                     >  SRR4302536.3131934/1‑94 (MQ=60)
                                                                        GTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGGGATTGGAATACA                    >  SRR4302536.3399515/1‑94 (MQ=60)
                                                                        GTAGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACA                    <  SRR4302536.15985/94‑1 (MQ=60)
                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                  <  SRR4302536.5191470/94‑1 (MQ=60)
                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                  <  SRR4302536.4770218/94‑1 (MQ=60)
                                                                          AGAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAT                  <  SRR4302536.5350425/94‑1 (MQ=60)
                                                                           GAGCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATT                 <  SRR4302536.6737864/94‑1 (MQ=60)
                                                                             GCTGGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTC               >  SRR4302536.3800661/1‑94 (MQ=60)
                                                                                GGACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCG            <  SRR4302536.5744380/94‑1 (MQ=60)
                                                                                 GACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGC           >  SRR4302536.1758092/1‑94 (MQ=60)
                                                                                  ACTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCC          >  SRR4302536.1759232/1‑94 (MQ=60)
                                                                                   CTTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCT         >  SRR4302536.1268356/1‑94 (MQ=60)
                                                                                    TTCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTT        <  SRR4302536.4491360/94‑1 (MQ=60)
                                                                                     TCAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTT       <  SRR4302536.4316947/94‑1 (MQ=60)
                                                                                      CAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTT      <  SRR4302536.6843136/94‑1 (MQ=60)
                                                                                      CAGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTT      <  SRR4302536.894868/94‑1 (MQ=60)
                                                                                       AGCCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTG     <  SRR4302536.3149248/94‑1 (MQ=60)
                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT   <  SRR4302536.1645165/94‑1 (MQ=60)
                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT   <  SRR4302536.6731819/94‑1 (MQ=60)
                                                                                         CCAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTT   >  SRR4302536.2341284/1‑94 (MQ=60)
                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT  >  SRR4302536.5363789/1‑94 (MQ=60)
                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT  >  SRR4302536.4059394/1‑94 (MQ=60)
                                                                                          CAGATTGAAAAAGCCTAACCCAGCGATAAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT  >  SRR4302536.4999505/1‑94 (MQ=60)
                                                                                             |                                                                                          
GTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTT  >  NC_000913/4175760‑4175943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑