Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,178,668 A→G intergenic (+165/‑155) rplL → / → rpoB 50S ribosomal subunit protein L7/L12/RNA polymerase beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,6680AG98.7% 750.4 / ‑2.9 237intergenic (+165/‑155)rplL/rpoB50S ribosomal subunit protein L7/L12/RNA polymerase beta subunit
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base G (124/110);  total (127/110)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

TGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAAT  >  NC_000913/4178578‑4178757
                                                                                          |                                                                                         
tGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCg                                                                                         >  1:929156/1‑93 (MQ=255)
tGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCg                                                                                         >  1:1615209/1‑93 (MQ=255)
tGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCg                                                                                         >  2:2863617/1‑93 (MQ=255)
 gTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGt                                                                                        >  1:1909071/1‑93 (MQ=255)
 gTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGt                                                                                        >  2:2148179/1‑93 (MQ=255)
   aaGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTcc                                                                                      >  1:4015370/1‑93 (MQ=255)
   aaGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTcc                                                                                      <  2:4376668/93‑1 (MQ=255)
   aaGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTcc                                                                                      <  2:3911040/93‑1 (MQ=255)
   aaGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTcc                                                                                      >  2:4107329/1‑93 (MQ=255)
     ggCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGt                                                                                    <  1:468886/93‑1 (MQ=255)
     ggCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGt                                                                                    <  2:3679228/93‑1 (MQ=255)
        gcCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCt                                                                                 >  1:3293130/1‑93 (MQ=255)
         ccAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCtg                                                                                <  2:3459620/93‑1 (MQ=255)
         ccAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCtg                                                                                >  1:3220792/1‑93 (MQ=255)
           aGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCtgtg                                                                              >  1:4335896/1‑93 (MQ=255)
            gTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTgtgt                                                                             >  1:247567/1‑93 (MQ=255)
            gTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTgtgt                                                                             <  1:351073/93‑1 (MQ=255)
            gTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTgtgt                                                                             >  2:674935/1‑93 (MQ=255)
               gCGTTTCACGCTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTaaa                                                                          >  2:741838/1‑93 (MQ=255)
               gCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTaaa                                                                          >  2:1750815/1‑93 (MQ=255)
                 gTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATc                                                                        >  2:1293062/1‑93 (MQ=255)
                 gTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATc                                                                        >  2:4518182/1‑93 (MQ=255)
                  tttCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCg                                                                       <  1:963980/93‑1 (MQ=255)
                   ttCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGc                                                                      >  1:3968242/1‑93 (MQ=255)
                     cacaCTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCaa                                                                    <  1:614015/93‑1 (MQ=255)
                       cacTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATg                                                                  >  1:4205745/1‑93 (MQ=255)
                        acTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGa                                                                 <  2:875263/93‑1 (MQ=255)
                          tGTTTGATTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGaaa                                                               <  1:4663653/93‑1 (MQ=255)
                          tGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGaaa                                                               >  1:22823/1‑93 (MQ=255)
                          tGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGaaa                                                               >  1:177792/1‑93 (MQ=255)
                           gTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATTGCAATGAAAt                                                              >  2:2685683/1‑93 (MQ=255)
                              tGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGt                                                           <  2:134577/93‑1 (MQ=255)
                                actactGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGttt                                                         >  2:3934440/1‑93 (MQ=255)
                                 ctactGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTa                                                        <  2:1839305/93‑1 (MQ=255)
                                  tactGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTaa                                                       <  1:3735763/93‑1 (MQ=255)
                                  tactGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTaa                                                       >  1:1604973/1‑93 (MQ=255)
                                  tactGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTaa                                                       >  1:1388869/1‑93 (MQ=255)
                                   actGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAg                                                      <  1:3667088/93‑1 (MQ=255)
                                    ctgctgTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGc                                                     >  2:1704489/1‑93 (MQ=255)
                                    ctgctgTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGc                                                     <  2:2194182/93‑1 (MQ=255)
                                    ctgctgTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGc                                                     <  2:3177896/93‑1 (MQ=255)
                                     cggtgtgCCTTTCAATGCTTGTTTCTATCGACTACTTAATATACTGCGACAGGGCGTCCTTTCTGTGTAAATCGCAATGAAATGGTTTAAGCg                                                    <  1:1277640/90‑1 (MQ=255)
                                      gctgTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGt                                                   >  2:3644400/1‑93 (MQ=255)
                                         gtgCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGAt                                                >  2:1560368/1‑93 (MQ=255)
                                          tgCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATa                                               <  1:4607344/93‑1 (MQ=255)
                                          tgCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATa                                               <  1:3173208/93‑1 (MQ=255)
                                          ggCCCTTCAATGCTTGTTTCGATCGACGACTTAATATACTGCGACAGGGCGTCCCGTCTGTGTAAATCGCAATTAAATGGTTTAAGCGTGATa                                               <  1:856654/92‑1 (MQ=255)
                                             cTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCa                                            >  1:3469993/1‑93 (MQ=255)
                                             cTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCa                                            >  2:4183894/1‑93 (MQ=255)
                                              tttCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCaa                                           >  1:4035682/1‑93 (MQ=255)
                                              tttCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCaa                                           >  2:504879/1‑93 (MQ=255)
                                                   aTGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGc                                      >  2:808177/1‑93 (MQ=255)
                                                    tGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCa                                     <  1:706610/93‑1 (MQ=255)
                                                    tGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCa                                     <  1:1948258/93‑1 (MQ=255)
                                                     gCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAt                                    >  2:593619/1‑93 (MQ=255)
                                                     gCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAt                                    <  1:3832617/93‑1 (MQ=255)
                                                      cTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAtt                                   <  1:876034/93‑1 (MQ=255)
                                                      cTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAtt                                   <  2:2268481/93‑1 (MQ=255)
                                                       ttGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTg                                  <  2:3395505/93‑1 (MQ=255)
                                                       ttGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTg                                  <  2:2556079/93‑1 (MQ=255)
                                                        tGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCTGGATAGCAACAGGCATTGc                                 <  2:3867394/93‑1 (MQ=255)
                                                         gTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCg                                <  1:2834183/93‑1 (MQ=255)
                                                         gTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCg                                >  2:1162514/1‑93 (MQ=255)
                                                         gTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCg                                >  1:1406528/1‑93 (MQ=255)
                                                          tttCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCgg                               <  1:457649/93‑1 (MQ=255)
                                                          tttCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCgg                               >  1:3627555/1‑93 (MQ=255)
                                                          tttCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCgg                               >  2:3092845/1‑93 (MQ=255)
                                                           ttCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGa                              >  2:1112218/1‑93 (MQ=255)
                                                             cTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCGATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGaaa                            >  2:1437724/1‑93 (MQ=255)
                                                             cTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGaaa                            <  2:1454575/93‑1 (MQ=255)
                                                              tATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAg                           >  2:1065572/1‑93 (MQ=255)
                                                              tATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAg                           >  1:2065589/1‑93 (MQ=255)
                                                               aTCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAgt                          <  1:3780086/93‑1 (MQ=255)
                                                               aTCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAgt                          <  2:3290645/93‑1 (MQ=255)
                                                                tCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAgtg                         >  1:2389426/1‑93 (MQ=255)
                                                                tCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAgtg                         >  1:3113979/1‑93 (MQ=255)
                                                                   acgacTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTc                      >  1:2162982/1‑93 (MQ=255)
                                                                   acgacTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTc                      <  1:4278940/93‑1 (MQ=255)
                                                                   acgacTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTc                      <  2:2392249/93‑1 (MQ=255)
                                                                     gacTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCa                    <  1:3146632/93‑1 (MQ=255)
                                                                      acTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAt                   >  2:3045646/1‑93 (MQ=255)
                                                                      acTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAt                   >  1:4162847/1‑93 (MQ=255)
                                                                      acTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAt                   >  1:2643509/1‑93 (MQ=255)
                                                                        ttAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAttt                 <  1:4008722/93‑1 (MQ=255)
                                                                        ttAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAttt                 <  2:719160/93‑1 (MQ=255)
                                                                         tAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAtttt                <  1:1228049/93‑1 (MQ=255)
                                                                          aaTATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTc               >  2:3318971/1‑93 (MQ=255)
                                                                             ataCTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCgg            <  2:3496497/93‑1 (MQ=255)
                                                                               aCTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTc          <  1:2782158/93‑1 (MQ=255)
                                                                                cTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTca         <  1:741838/93‑1 (MQ=255)
                                                                                cTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTca         <  1:4109606/93‑1 (MQ=255)
                                                                                 tGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaa        <  1:84597/93‑1 (MQ=255)
                                                                                 tGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaa        <  1:3915380/93‑1 (MQ=255)
                                                                                  gCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaac       <  2:1620400/93‑1 (MQ=255)
                                                                                  gCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaac       <  2:2486156/93‑1 (MQ=255)
                                                                                    gACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaacaa     >  1:1072969/1‑93 (MQ=255)
                                                                                    gACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTcaacaa     >  2:2605953/1‑93 (MQ=255)
                                                                                     aCAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACaaa    >  2:4655574/1‑93 (MQ=255)
                                                                                      cAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACaaaa   >  2:3124659/1‑93 (MQ=255)
                                                                                       aGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAAt  >  2:4286081/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAAT  >  NC_000913/4178578‑4178757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTG  >  NC_000913/4178575‑4178761
                                                                                             |                                                                                             
CGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGG                                                                                               <  SRR4302544.3473852/94‑1 (MQ=60)
CGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGG                                                                                               >  SRR4302544.3543407/1‑94 (MQ=60)
CGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGG                                                                                               >  SRR4302544.1075862/1‑94 (MQ=60)
AGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGG                                                                                               >  SRR4302544.4323072/1‑94 (MQ=60)
 GCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGC                                                                                              >  SRR4302544.663786/1‑94 (MQ=60)
  CTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCG                                                                                             <  SRR4302544.240089/94‑1 (MQ=60)
  CTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCG                                                                                             >  SRR4302544.938309/1‑94 (MQ=60)
  CTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCG                                                                                             >  SRR4302544.1631131/1‑94 (MQ=60)
   TGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGT                                                                                            >  SRR4302544.2343539/1‑94 (MQ=60)
   TGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGT                                                                                            >  SRR4302544.1927525/1‑94 (MQ=60)
     TAAGGCGCCAGTAGTCGTTCACAATGTTTGGCTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCC                                                                                          <  SRR4302544.222501/94‑1 (MQ=60)
     TAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCC                                                                                          <  SRR4302544.3916255/94‑1 (MQ=60)
     TAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCC                                                                                          >  SRR4302544.4055306/1‑94 (MQ=60)
     TAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCC                                                                                          >  SRR4302544.606594/1‑94 (MQ=60)
      AAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCG                                                                                         <  SRR4302544.3774605/94‑1 (MQ=60)
        GGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTT                                                                                       <  SRR4302544.4637402/94‑1 (MQ=60)
        GGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTT                                                                                       <  SRR4302544.473476/94‑1 (MQ=60)
          CGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATNNNNTGCGACAGGGNGNCCGTNCN                                                                                     >  SRR4302544.1425104/1‑94 (MQ=60)
          CGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCT                                                                                     >  SRR4302544.3324496/1‑94 (MQ=60)
           GCCAGTAGCGTTTCACACTGTTTGACTACTTCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTG                                                                                    <  SRR4302544.3741681/94‑1 (MQ=60)
           GCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTG                                                                                    <  SRR4302544.4505852/94‑1 (MQ=60)
           GCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTG                                                                                    >  SRR4302544.3251529/1‑94 (MQ=60)
             CAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTG                                                                                  >  SRR4302544.4380027/1‑94 (MQ=60)
              AGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGT                                                                                 >  SRR4302544.4668060/1‑94 (MQ=60)
              AGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGT                                                                                 >  SRR4302544.250161/1‑94 (MQ=60)
              AGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTCAATATACTGCGACAGGGCGTCCGTTCTGTGT                                                                                 >  SRR4302544.4017841/1‑94 (MQ=60)
               GTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTA                                                                                <  SRR4302544.4398946/94‑1 (MQ=60)
               GTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTA                                                                                <  SRR4302544.354671/94‑1 (MQ=60)
               GTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTA                                                                                <  SRR4302544.354673/94‑1 (MQ=60)
               GTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTA                                                                                <  SRR4302544.996819/94‑1 (MQ=60)
                 AGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAA                                                                              <  SRR4302544.4181700/94‑1 (MQ=60)
                  GCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAAT                                                                             <  SRR4302544.1513072/94‑1 (MQ=60)
                   GGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATC                                                                            <  SRR4302544.1904430/94‑1 (MQ=60)
                   CGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATC                                                                            >  SRR4302544.2344505/1‑94 (MQ=60)
                     TTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGC                                                                          <  SRR4302544.973442/94‑1 (MQ=60)
                     TTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGC                                                                          >  SRR4302544.4007712/1‑94 (MQ=60)
                      TTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCA                                                                         <  SRR4302544.390822/94‑1 (MQ=60)
                        CACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAAT                                                                       <  SRR4302544.620037/94‑1 (MQ=60)
                        CACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAAT                                                                       <  SRR4302544.3242274/94‑1 (MQ=60)
                         ACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATG                                                                      >  SRR4302544.4247927/1‑94 (MQ=60)
                           ACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAA                                                                    <  SRR4302544.2517962/94‑1 (MQ=60)
                            CTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAA                                                                   >  SRR4302544.1274981/1‑94 (MQ=60)
                            CTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAA                                                                   >  SRR4302544.179553/1‑94 (MQ=60)
                            CTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAA                                                                   >  SRR4302544.23045/1‑94 (MQ=60)
                             TGTTTGATTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAAT                                                                  <  SRR4302544.4709779/94‑1 (MQ=60)
                              GTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATG                                                                 >  SRR4302544.682198/1‑94 (MQ=60)
                               TTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGG                                                                <  SRR4302544.1833490/94‑1 (MQ=60)
                                   ACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTA                                                            <  SRR4302544.4207704/94‑1 (MQ=60)
                                    CTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAA                                                           >  SRR4302544.1402736/1‑94 (MQ=60)
                                    CTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAA                                                           >  SRR4302544.3619010/1‑94 (MQ=60)
                                    CTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAA                                                           >  SRR4302544.1620799/1‑94 (MQ=60)
                                    CTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAA                                                           >  SRR4302544.2142436/1‑94 (MQ=60)
                                     TACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAG                                                          <  SRR4302544.3772901/94‑1 (MQ=60)
                                      ACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGC                                                         <  SRR4302544.3703657/94‑1 (MQ=60)
                                        cggTGTGCCTTTCAATGCTTGTTTCTATCGACTACTTAATATACTGCGACAGGGCGTCCTTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGT                                                       <  SRR4302544.1290221/91‑1 (MQ=60)
                                        TGCTGTTCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGT                                                       <  SRR4302544.1290341/94‑1 (MQ=60)
                                         GCTGTGCCTTTCAATCCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTG                                                      <  SRR4302544.3087162/94‑1 (MQ=60)
                                          CTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGA                                                     <  SRR4302544.1985268/94‑1 (MQ=60)
                                          CTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGA                                                     >  SRR4302544.4070617/1‑94 (MQ=60)
                                           TGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGAT                                                    >  SRR4302544.4751834/1‑94 (MQ=60)
                                             ggcccTTCAATGCTTGTTTCGATCGACGACTTAATATACTGCGACAGGGCGTCCCGTCTGTGTAAATCGCAATTAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.865128/89‑1 (MQ=60)
                                             TGTCTTTCAATTGTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCGCTGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.865127/94‑1 (MQ=60)
                                             TGCCTTTCAATTCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAAGCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.3301161/94‑1 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.4653114/94‑1 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  >  SRR4302544.949409/1‑94 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.865129/94‑1 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.3301160/94‑1 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.3149628/94‑1 (MQ=60)
                                             TGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAG                                                  <  SRR4302544.3203402/94‑1 (MQ=60)
                                               CCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCA                                                >  SRR4302544.2825716/1‑94 (MQ=60)
                                               CCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCA                                                >  SRR4302544.1353602/1‑94 (MQ=60)
                                               CCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCA                                                >  SRR4302544.3503082/1‑94 (MQ=60)
                                               CCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCA                                                >  SRR4302544.881818/1‑94 (MQ=60)
                                                CTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAA                                               >  SRR4302544.891241/1‑94 (MQ=60)
                                                CTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAA                                               >  SRR4302544.4075874/1‑94 (MQ=60)
                                                CTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAA                                               >  SRR4302544.2595430/1‑94 (MQ=60)
                                                  TTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACA                                             >  SRR4302544.1106439/1‑94 (MQ=60)
                                                    CAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGG                                           <  SRR4302544.799110/94‑1 (MQ=60)
                                                       TGCTTGTTTCTATCGATGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAT                                        >  SRR4302544.2752354/1‑94 (MQ=60)
                                                       TGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAtgaaaatggttaaacgaggatgcaacaaggact                                        >  SRR4302544.1928484/1‑61 (MQ=60)
                                                       TGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAT                                        <  SRR4302544.713629/94‑1 (MQ=60)
                                                       TGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAT                                        <  SRR4302544.1967129/94‑1 (MQ=60)
                                                       TGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAT                                        >  SRR4302544.1928483/1‑94 (MQ=60)
                                                        GCTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATT                                       <  SRR4302544.3870614/94‑1 (MQ=60)
                                                         CTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTG                                      >  SRR4302544.3190297/1‑94 (MQ=60)
                                                         CTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTG                                      <  SRR4302544.3994375/94‑1 (MQ=60)
                                                         CTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTG                                      <  SRR4302544.4662947/94‑1 (MQ=60)
                                                         CTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTG                                      >  SRR4302544.735366/1‑94 (MQ=60)
                                                         CTTGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTG                                      <  SRR4302544.884726/94‑1 (MQ=60)
                                                           TGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCG                                    >  SRR4302544.1420532/1‑94 (MQ=60)
                                                           TGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCG                                    >  SRR4302544.1420533/1‑94 (MQ=60)
                                                           TGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCG                                    >  SRR4302544.1436643/1‑94 (MQ=60)
                                                           TGTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCG                                    <  SRR4302544.2916589/94‑1 (MQ=60)
                                                            GTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCACCAGGCATTGCGG                                   >  SRR4302544.3663696/1‑94 (MQ=60)
                                                            GTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGG                                   >  SRR4302544.3663694/1‑94 (MQ=60)
                                                            GTTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGG                                   <  SRR4302544.2861565/94‑1 (MQ=60)
                                                             TTTCTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGA                                  <  SRR4302544.462155/94‑1 (MQ=60)
                                                                CTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAG                               <  SRR4302544.780054/94‑1 (MQ=60)
                                                                CTATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAG                               >  SRR4302544.2085439/1‑94 (MQ=60)
                                                                 TATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGT                              >  SRR4302544.4052379/1‑94 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCTGAAAGTG                             >  SRR4302544.2489967/1‑94 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTG                             <  SRR4302544.3817526/94‑1 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTG                             >  SRR4302544.2412055/1‑94 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTG                             >  SRR4302544.2052056/1‑94 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTG                             >  SRR4302544.3143600/1‑94 (MQ=60)
                                                                  ATCGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTG                             >  SRR4302544.1679461/1‑94 (MQ=60)
                                                                    CGACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTT                           >  SRR4302544.4208823/1‑94 (MQ=60)
                                                                     GACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTC                          >  SRR4302544.2183515/1‑94 (MQ=60)
                                                                     GACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTC                          >  SRR4302544.3504217/1‑94 (MQ=60)
                                                                      ACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCC                         <  SRR4302544.4322639/94‑1 (MQ=60)
                                                                      ACGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCC                         <  SRR4302544.402144/94‑1 (MQ=60)
                                                                       CGACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCA                        >  SRR4302544.4099408/1‑94 (MQ=60)
                                                                        GACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAT                       <  SRR4302544.3176546/94‑1 (MQ=60)
                                                                        GACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAT                       >  SRR4302544.2668855/1‑94 (MQ=60)
                                                                        GACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCAT                       >  SRR4302544.4204083/1‑94 (MQ=60)
                                                                        GACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCAATGCGGAAAGTGTTCCAT                       >  SRR4302544.4267225/1‑94 (MQ=60)
                                                                         ACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATT                      <  SRR4302544.3361035/94‑1 (MQ=60)
                                                                         ACTTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATT                      <  SRR4302544.4379758/94‑1 (MQ=60)
                                                                           TTAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTT                    <  SRR4302544.4048602/94‑1 (MQ=60)
                                                                            TAATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTC                   <  SRR4302544.1240201/94‑1 (MQ=60)
                                                                              ATATACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCG                 >  SRR4302544.4100341/1‑94 (MQ=60)
                                                                                  ACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCA             <  SRR4302544.3185697/94‑1 (MQ=60)
                                                                                  ACTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCA             <  SRR4302544.2808934/94‑1 (MQ=60)
                                                                                   CTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAA            <  SRR4302544.4150457/94‑1 (MQ=60)
                                                                                   CTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAA            >  SRR4302544.3853377/1‑94 (MQ=60)
                                                                                   CTGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAA            <  SRR4302544.749102/94‑1 (MQ=60)
                                                                                    TGCGAGAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAAC           <  SRR4302544.1623051/94‑1 (MQ=60)
                                                                                    TGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAAC           <  SRR4302544.3954274/94‑1 (MQ=60)
                                                                                    TGCGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAAC           <  SRR4302544.85401/94‑1 (MQ=60)
                                                                                      CGACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAA         >  SRR4302544.1083635/1‑94 (MQ=60)
                                                                                        ACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAA       >  SRR4302544.3762812/1‑94 (MQ=60)
                                                                                        ACAGGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAA       <  SRR4302544.4710483/94‑1 (MQ=60)
                                                                                           GGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAG    <  SRR4302544.4019132/94‑1 (MQ=60)
                                                                                           GGGCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAG    <  SRR4302544.4632022/94‑1 (MQ=60)
                                                                                             GCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTG  <  SRR4302544.3905521/94‑1 (MQ=60)
                                                                                             GCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTG  <  SRR4302544.1228713/94‑1 (MQ=60)
                                                                                             GCGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTG  <  SRR4302544.1228712/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGCTGTAAGGCGCCAGTAGCGTTTCACACTGTTTGACTACTGCTGTGCCTTTCAATGCTTGTTTCTATCGACGACTTAATATACTGCGACAGGACGTCCGTTCTGTGTAAATCGCAATGAAATGGTTTAAGCGTGATAGCAACAGGCATTGCGGAAAGTGTTCCATTTTCCGGTCAACAAAATAGTG  >  NC_000913/4178575‑4178761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑