Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,244,543 T→C F61L (TTC→CTC)  malK → ATP‑binding component of transport system for maltose

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,5430TC96.0% 448.0 / 5.7 149F61L (TTC→CTC) malKATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base C (61/82);  total (63/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01

Only 100 of 151 total aligned reads displayed.

CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT  >  NC_000913/4244455‑4244631
                                                                                        |                                                                                        
 gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     <  1:429125/93‑1 (MQ=255)
 gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     <  1:429124/93‑1 (MQ=255)
 gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     >  2:215742/1‑93 (MQ=255)
 gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     <  2:4300587/93‑1 (MQ=255)
 gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     >  2:3073394/1‑93 (MQ=255)
 gtggtgTTGGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                     <  1:1074154/93‑1 (MQ=255)
   ggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCgg                                                                                   >  2:2112692/1‑93 (MQ=255)
   ggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCgg                                                                                   <  1:3194615/93‑1 (MQ=255)
    gtgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGt                                                                                  <  2:2525021/93‑1 (MQ=255)
     tgtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTg                                                                                 >  1:2194151/1‑93 (MQ=255)
      gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATTATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCCGTgg                                                                                >  2:3813792/1‑92 (MQ=39)
         tGTCGGCCCGTCTGGCTGCGGT‑AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaaa                                                                            <  1:2597108/93‑1 (MQ=37)
           tCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAc                                                                           <  2:3793522/93‑1 (MQ=255)
            cGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACg                                                                          <  1:606912/93‑1 (MQ=255)
              gACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGa                                                                        <  2:3417037/93‑1 (MQ=255)
              gACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGa                                                                        <  2:4510026/93‑1 (MQ=255)
              gACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGa                                                                        <  2:3417036/93‑1 (MQ=255)
              gACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGa                                                                        <  1:1597265/93‑1 (MQ=255)
              gACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGa                                                                        <  2:2174063/93‑1 (MQ=255)
               aCCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGat                                                                       <  2:2035482/93‑1 (MQ=255)
                 cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatga                                                                     >  1:1423523/1‑93 (MQ=255)
                  gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGGCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                    <  2:2695871/93‑1 (MQ=255)
                  gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                    <  1:3159170/93‑1 (MQ=255)
                  gTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaa                                                                    >  2:691176/1‑93 (MQ=255)
                   tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                   <  1:1206128/93‑1 (MQ=255)
                     tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                 >  1:210673/1‑93 (MQ=255)
                     tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                 >  2:4300300/1‑93 (MQ=255)
                       gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                               <  1:3620303/93‑1 (MQ=255)
                       gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                               >  2:2495911/1‑93 (MQ=255)
                        cTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGacac                                                              <  2:4138551/93‑1 (MQ=255)
                          gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                            >  2:290672/1‑93 (MQ=255)
                           cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                           >  2:569439/1‑93 (MQ=255)
                           cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                           <  2:122820/93‑1 (MQ=255)
                           cGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTcc                                                           <  1:4421228/93‑1 (MQ=255)
                             gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                         >  2:4011691/1‑93 (MQ=255)
                               aaaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCca                                                       >  1:2007267/1‑93 (MQ=255)
                               aaaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGccg                                                       >  1:1564957/1‑92 (MQ=255)
                                aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcag                                                      >  2:2655985/1‑93 (MQ=255)
                                aaTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGAGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCCCcac                                                      >  2:3997844/1‑92 (MQ=38)
                                 aTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagc                                                     <  1:597634/93‑1 (MQ=255)
                                   cGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagcag                                                   >  2:2816606/1‑93 (MQ=255)
                                   cGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagcag                                                   >  1:4428802/1‑93 (MQ=255)
                                    gACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGa                                                  >  1:1933976/1‑93 (MQ=255)
                                     aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                 <  1:379217/93‑1 (MQ=255)
                                      cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTTATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                <  2:1555187/93‑1 (MQ=255)
                                      cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                <  2:4419912/93‑1 (MQ=255)
                                      cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                <  1:2403678/93‑1 (MQ=255)
                                       tttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcg                                               >  1:2936933/1‑93 (MQ=255)
                                        ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                              <  2:3827374/93‑1 (MQ=255)
                                         tACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgcg                                             >  1:3965667/1‑93 (MQ=255)
                                          aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                            >  1:2456448/1‑93 (MQ=255)
                                          aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                            <  1:3640637/93‑1 (MQ=255)
                                           cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                           >  1:2691392/1‑93 (MQ=255)
                                           cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                           <  2:4446121/93‑1 (MQ=255)
                                            tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                          >  1:684979/1‑93 (MQ=255)
                                            tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                          >  2:3169929/1‑93 (MQ=255)
                                             gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                         <  2:3110335/93‑1 (MQ=255)
                                             gcgcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGt                                         <  2:4160979/93‑1 (MQ=255)
                                               gcATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGCCGTTg                                       >  1:1653494/1‑93 (MQ=255)
                                                cATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTgg                                      >  2:242185/1‑93 (MQ=255)
                                                 aTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACCCTCCGCCAGCAGAACGCGGCGTTGGt                                     <  2:3756092/93‑1 (MQ=255)
                                                 aTGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGt                                     >  1:776532/1‑93 (MQ=255)
                                                   gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                   >  2:3624819/1‑93 (MQ=255)
                                                   gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                   <  2:2732421/93‑1 (MQ=255)
                                                     ttGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATgg                                 <  1:2173351/93‑1 (MQ=255)
                                                      tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                <  2:2712162/93‑1 (MQ=255)
                                                      tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                <  1:4231306/93‑1 (MQ=255)
                                                      tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                <  2:1238362/93‑1 (MQ=255)
                                                      tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                <  1:900596/93‑1 (MQ=255)
                                                       gCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGCACGCGGCGTTGGTATGgtg                               >  1:1745377/1‑93 (MQ=255)
                                                       gCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtg                               <  1:665837/93‑1 (MQ=255)
                                                        gcGGGCGTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtgt                              <  1:3491702/92‑1 (MQ=255)
                                                         ccggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtt                             <  2:3238599/91‑1 (MQ=255)
                                                         cGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGtt                             >  1:3332020/1‑93 (MQ=255)
                                                             cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                         <  1:3815976/93‑1 (MQ=255)
                                                              ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                        >  2:3842721/1‑93 (MQ=255)
                                                                gagaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCt                      <  1:2389523/93‑1 (MQ=255)
                                                                gagaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCt                      >  1:1865882/1‑93 (MQ=255)
                                                                 agaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCtt                     >  2:66117/1‑93 (MQ=255)
                                                                  gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                    >  2:507586/1‑93 (MQ=255)
                                                                  gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                    <  2:2036775/93‑1 (MQ=255)
                                                                   aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                   <  2:2523471/93‑1 (MQ=255)
                                                                     gATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgc                 <  2:2094967/93‑1 (MQ=255)
                                                                     gATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgc                 >  1:3830341/1‑93 (MQ=255)
                                                                      aTCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcgcg                >  2:2895427/1‑93 (MQ=255)
                                                                       tCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACgcgc               <  1:2761755/93‑1 (MQ=255)
                                                                        cACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATTAATGCCACTCCGCCAGCAGGACCCGGCGTTGGTATGGGGTTTCATTCTTACGCGct              >  1:3684909/1‑93 (MQ=255)
                                                                        cACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGct              >  1:3684910/1‑93 (MQ=255)
                                                                         aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc             <  2:4512460/93‑1 (MQ=255)
                                                                         aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc             >  1:2874723/1‑93 (MQ=255)
                                                                           cAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGAATGGTGTTTCAGTCTTACGCGCTCTa           >  1:1971347/1‑93 (MQ=255)
                                                                              cggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcc        <  1:1118857/93‑1 (MQ=255)
                                                                               ggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATccc       <  2:1546961/93‑1 (MQ=255)
                                                                                gcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc      <  1:1795284/93‑1 (MQ=255)
                                                                                gcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATcccc      <  2:929514/93‑1 (MQ=255)
                                                                                   aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc   <  1:829438/93‑1 (MQ=255)
                                                                                   aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc   <  1:3329209/93‑1 (MQ=255)
                                                                                    ccTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCCGCAGAACGCGGCGGTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt  >  2:2810196/1‑93 (MQ=255)
                                                                                    ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAGCGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt  <  1:4232631/93‑1 (MQ=255)
                                                                                    ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt  >  1:3494621/1‑93 (MQ=255)
                                                                                        |                                                                                        
CGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT  >  NC_000913/4244455‑4244631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244450‑4244636
                                                                                             |                                                                                             
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGC                                                                                               >  SRR4302544.400762/1‑94 (MQ=60)
 AATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCT                                                                                              >  SRR4302544.3815986/1‑94 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         >  SRR4302544.3292797/1‑94 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302544.433478/94‑1 (MQ=60)
      GTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302544.433479/94‑1 (MQ=60)
      GTGGTGTTGGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         <  SRR4302544.1084829/94‑1 (MQ=60)
      GCGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCG                                                                                         >  SRR4302544.1211025/1‑94 (MQ=60)
       GGGTGTTTGTGGGACCGTCTGGCTGCTGTAAATCGACTTTACTGCGCATGATTGCCGGTCTTGAGACGATCACCAGCGGCGACCTGCTCATCGG                                                                                        <  SRR4302544.2458509/94‑1 (MQ=60)
        GGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGT                                                                                       <  SRR4302544.3225056/94‑1 (MQ=60)
         GTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTG                                                                                      >  SRR4302544.2214857/1‑94 (MQ=60)
           GTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGCG                                                                                    >  SRR4302544.1780835/1‑94 (MQ=60)
             TTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAA                                                                                  <  SRR4302544.3562564/94‑1 (MQ=60)
              TGTCGGCCCGTCTGGCTGCGGT‑AATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAC                                                                                <  SRR4302544.2622077/94‑1 (MQ=60)
              TGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAA                                                                                 >  SRR4302544.874959/1‑94 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302544.561902/94‑1 (MQ=60)
                 CGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGG                                                                              <  SRR4302544.612869/94‑1 (MQ=60)
                   GACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGAT                                                                            <  SRR4302544.1613003/94‑1 (MQ=60)
                     CCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGA                                                                          >  SRR4302544.1437686/1‑94 (MQ=60)
                       GTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAAT                                                                        <  SRR4302544.3189217/94‑1 (MQ=60)
                        TCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATG                                                                       <  SRR4302544.1218073/94‑1 (MQ=60)
                         CTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGA                                                                      >  SRR4302544.212844/1‑94 (MQ=60)
                          TGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAC                                                                     <  SRR4302544.999465/94‑1 (MQ=60)
                            GCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACAC                                                                   <  SRR4302544.3656371/94‑1 (MQ=60)
                               GCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCC                                                                <  SRR4302544.1896390/94‑1 (MQ=60)
                                CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCG                                                               <  SRR4302544.4465840/94‑1 (MQ=60)
                                CGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCAACAGCGGCGACCTGTTCATCGGTGTGAAACGGATGAATGACACTCCA                                                               >  SRR4302544.2803712/1‑94 (MQ=60)
                                   TAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCA                                                            >  SRR4302544.2026772/1‑94 (MQ=60)
                                   TAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCG                                                            >  SRR4302544.1580477/1‑94 (MQ=60)
                                      ATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCA                                                         <  SRR4302544.603490/94‑1 (MQ=60)
                                       TCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAG                                                        >  SRR4302544.4473488/1‑94 (MQ=60)
                                        CGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGA                                                       >  SRR4302544.1952695/1‑94 (MQ=60)
                                         GACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAA                                                      >  SRR4302544.4286904/1‑94 (MQ=60)
                                          ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302544.413594/94‑1 (MQ=60)
                                          ACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAC                                                     <  SRR4302544.383078/94‑1 (MQ=60)
                                           CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    >  SRR4302544.2965138/1‑94 (MQ=60)
                                           CTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACG                                                    <  SRR4302544.2426492/94‑1 (MQ=60)
                                             TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  >  SRR4302544.4005117/1‑94 (MQ=60)
                                             TTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCG                                                  <  SRR4302544.1641780/94‑1 (MQ=60)
                                              TACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGG                                                 >  SRR4302544.2479882/1‑94 (MQ=60)
                                               ACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGC                                                >  SRR4302544.2717209/1‑94 (MQ=60)
                                               ACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGC                                                <  SRR4302544.3676953/94‑1 (MQ=60)
                                               ACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGC                                                >  SRR4302544.4369699/1‑94 (MQ=60)
                                                CTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCG                                               >  SRR4302544.691791/1‑94 (MQ=60)
                                                   CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTG                                            <  SRR4302544.3699893/94‑1 (MQ=60)
                                                   CGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGCCGTTG                                            >  SRR4302544.1669802/1‑94 (MQ=60)
                                                     CATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGT                                          >  SRR4302544.784069/1‑94 (MQ=60)
                                                       TGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAT                                        <  SRR4302544.37391/94‑1 (MQ=60)
                                                        GATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATG                                       <  SRR4302544.1580040/94‑1 (MQ=60)
                                                        GATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATG                                       <  SRR4302544.2133711/94‑1 (MQ=60)
                                                          TTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGT                                     <  SRR4302544.2193949/94‑1 (MQ=60)
                                                           TGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGCACGCGGCGTTGGTATGGTG                                    >  SRR4302544.1762464/1‑94 (MQ=60)
                                                           TGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTG                                    <  SRR4302544.4274418/94‑1 (MQ=60)
                                                           TGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTG                                    <  SRR4302544.909507/94‑1 (MQ=60)
                                                            TCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGT                                   <  SRR4302544.672410/94‑1 (MQ=60)
                                                            GCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGT                                   <  SRR4302544.672411/94‑1 (MQ=60)
                                                             GCGGGCGTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTT                                  <  SRR4302544.3524994/94‑1 (MQ=60)
                                                             CCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTT                                  >  SRR4302544.3363751/1‑94 (MQ=60)
                                                             CCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGCTGGTATGGTGTT                                  >  SRR4302544.3859747/1‑94 (MQ=60)
                                                                GGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCA                               <  SRR4302544.2003943/94‑1 (MQ=60)
                                                                  CTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGT                             <  SRR4302544.3853798/94‑1 (MQ=60)
                                                                    TGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCT                           >  SRR4302544.1883911/1‑94 (MQ=60)
                                                                     GAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTT                          <  SRR4302544.2412152/94‑1 (MQ=60)
                                                                      AGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTA                         >  SRR4302544.3098506/1‑94 (MQ=60)
                                                                       GACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAC                        <  SRR4302544.3437613/94‑1 (MQ=60)
                                                                         CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      >  SRR4302544.3868323/1‑94 (MQ=60)
                                                                         CGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGC                      <  SRR4302544.2639266/94‑1 (MQ=60)
                                                                            TCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATTAATGCCACTCCGCCAGCAGGACCCGGCGTTGGTATGGGGTTTCATTCTTACGCGCT                   >  SRR4302544.3721647/1‑94 (MQ=60)
                                                                            TCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCT                   <  SRR4302544.2788321/94‑1 (MQ=60)
                                                                            TCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCT                   >  SRR4302544.3721648/1‑94 (MQ=60)
                                                                             CACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTC                  >  SRR4302544.2902471/1‑94 (MQ=60)
                                                                               CCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGAATGGTGTTTCAGTCTTACGCGCTCTA                >  SRR4302544.1990502/1‑94 (MQ=60)
                                                                                 AGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATC              >  SRR4302544.752598/1‑94 (MQ=60)
                                                                                   CGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCC            <  SRR4302544.1130019/94‑1 (MQ=60)
                                                                                     GCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCA          <  SRR4302544.1812838/94‑1 (MQ=60)
                                                                                       GACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACC        <  SRR4302544.2268374/94‑1 (MQ=60)
                                                                                        ACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT       >  SRR4302544.3527942/1‑94 (MQ=60)
                                                                                        ACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT       <  SRR4302544.837584/94‑1 (MQ=60)
                                                                                        ACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCT       <  SRR4302544.3360910/94‑1 (MQ=60)
                                                                                         CCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAGCGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG      <  SRR4302544.4275759/94‑1 (MQ=60)
                                                                                           TGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTC    <  SRR4302544.4399533/94‑1 (MQ=60)
                                                                                           TGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTC    >  SRR4302544.798646/1‑94 (MQ=60)
                                                                                           TGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGAAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTC    >  SRR4302544.423472/1‑94 (MQ=60)
                                                                                            GCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCA   <  SRR4302544.2022964/94‑1 (MQ=60)
                                                                                            GCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCA   <  SRR4302544.2022963/94‑1 (MQ=60)
                                                                                             CTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  SRR4302544.362185/1‑94 (MQ=60)
                                                                                             CTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGCTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  <  SRR4302544.1329549/94‑1 (MQ=60)
                                                                                             |                                                                                             
GAATTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTGTCAG  >  NC_000913/4244450‑4244636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑