Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,246,188 A→G E213E (GAA→GAG)  lamB → phage lambda receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,246,1880AG98.0% 648.5 / ‑0.1 205E213E (GAA→GAG) lamBphage lambda receptor protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base G (97/104);  total (100/105)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 215 total aligned reads displayed.

CAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACA  >  NC_000913/4246098‑4246280
                                                                                          |                                                                                            
 aaTATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTg                                                                                           <  1:2512438/93‑1 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          <  1:3539807/93‑1 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          >  2:3469372/1‑93 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          >  1:3973098/1‑93 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          <  2:4396182/93‑1 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          <  2:411868/93‑1 (MQ=255)
  atatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTgg                                                                                          <  2:2530962/93‑1 (MQ=255)
   tatTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTggg                                                                                         <  1:4360345/93‑1 (MQ=255)
     tttATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGgtg                                                                                       <  2:2900931/93‑1 (MQ=255)
     tttATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGgtg                                                                                       >  1:2914307/1‑93 (MQ=255)
     tttATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGgtg                                                                                       <  1:2222430/93‑1 (MQ=255)
        aTGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCg                                                                                    >  2:3509793/1‑93 (MQ=255)
         tGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGa                                                                                   <  2:3117794/93‑1 (MQ=255)
         tGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGa                                                                                   >  2:1156028/1‑93 (MQ=255)
         tGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGa                                                                                   <  2:1716091/93‑1 (MQ=255)
          gACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGAc                                                                                  >  2:1292839/1‑93 (MQ=255)
           aCTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACt                                                                                 >  1:3328286/1‑93 (MQ=255)
           aCTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACt                                                                                 <  2:2099089/93‑1 (MQ=255)
            cTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTa                                                                                <  2:2301932/93‑1 (MQ=255)
             tataCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTAc                                                                               >  1:4254111/1‑93 (MQ=255)
             tataCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTAc                                                                               >  2:1099466/1‑93 (MQ=255)
              ataCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCACCCCGGGCGGCCCATTAGAGCTGGGTGTCGACTACg                                                                              >  1:706995/1‑93 (MQ=255)
              ataCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACg                                                                              >  1:4380719/1‑93 (MQ=255)
              ataCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACg                                                                              >  2:2476516/1‑93 (MQ=255)
               taCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACgg                                                                             <  1:3894979/93‑1 (MQ=255)
               taCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACgg                                                                             >  2:2846755/1‑93 (MQ=255)
               taCCAACGAAACCGCGAACGACGTTTTCGATGGGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACgg                                                                             <  1:1586788/93‑1 (MQ=255)
                aCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGt                                                                            >  2:2027469/1‑93 (MQ=255)
                aCCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGt                                                                            <  1:3495772/93‑1 (MQ=255)
                  cAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGCCg                                                                          >  2:872055/1‑93 (MQ=255)
                   aaCGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGt                                                                         <  2:380995/93‑1 (MQ=255)
                      gAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGcc                                                                      >  1:3734979/1‑93 (MQ=255)
                        aaCCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCaa                                                                    >  1:3147298/1‑93 (MQ=255)
                        aaCCGCGAACGACGCTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGCCGGCACATTAGAGCTGGGTGTCGACTACGGACGTCCCac                                                                    >  2:2509339/1‑92 (MQ=255)
                           cgcgAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACtt                                                                 <  2:3316762/93‑1 (MQ=255)
                            gcgAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTg                                                                <  2:4538533/93‑1 (MQ=255)
                            gcgAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTg                                                                >  1:1524078/1‑93 (MQ=255)
                            gcgAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTg                                                                >  2:3536405/1‑93 (MQ=255)
                            gcgAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCCGGCGGCACATTAGAGATGGGCGTCGACACCGGTCGTGCCAACTTg                                                                >  2:650237/1‑93 (MQ=255)
                             cgAACGACGTTTTCGATTTGCGTTTAGCGCAGATGGAAATCAACCCGTGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGc                                                               >  2:3627420/1‑93 (MQ=255)
                              gAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCg                                                              >  2:1532037/1‑93 (MQ=255)
                              gAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCg                                                              >  2:1791441/1‑93 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             <  2:3417947/93‑1 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             >  1:688032/1‑93 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             <  1:3181438/93‑1 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             <  2:2950539/93‑1 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             >  2:2996641/1‑93 (MQ=255)
                               aaCGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGt                                                             <  1:1299858/93‑1 (MQ=255)
                                acgacgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTg                                                            >  1:1625670/1‑93 (MQ=255)
                                 cgacgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCCACTTGCGTGa                                                           >  2:4315/1‑93 (MQ=255)
                                 cgacgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGa                                                           >  1:2224731/1‑93 (MQ=255)
                                   acgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATa                                                         <  1:2369002/93‑1 (MQ=255)
                                   acgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATa                                                         >  1:3124381/1‑93 (MQ=255)
                                   acgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATa                                                         <  1:3580651/93‑1 (MQ=255)
                                    cgTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATaa                                                        <  1:1675197/93‑1 (MQ=255)
                                      ttttCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACt                                                      >  1:4474322/1‑93 (MQ=255)
                                      ttttCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACt                                                      >  2:420313/1‑93 (MQ=255)
                                       tttCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTa                                                     <  1:3249551/93‑1 (MQ=255)
                                            aTGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTc                                                >  1:552286/1‑93 (MQ=255)
                                             tgtgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCt                                               <  1:3191242/93‑1 (MQ=255)
                                             tgtgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCt                                               >  2:2385981/1‑93 (MQ=255)
                                              gtgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTg                                              >  1:4324529/1‑93 (MQ=255)
                                              gtgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTg                                              <  1:4014789/93‑1 (MQ=255)
                                               tgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTgg                                             <  2:2814276/93‑1 (MQ=255)
                                               tgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTgg                                             <  1:483569/93‑1 (MQ=255)
                                               tgCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTgg                                             <  1:2490627/93‑1 (MQ=255)
                                                 cGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGtt                                           <  1:79844/93‑1 (MQ=255)
                                                 cGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGtt                                           >  1:2813640/1‑93 (MQ=255)
                                                   tttAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGa                                         <  1:2344213/93‑1 (MQ=255)
                                                     tAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGTGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATg                                       <  1:3064702/93‑1 (MQ=255)
                                                     tAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATg                                       <  2:1668660/93‑1 (MQ=255)
                                                     tAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATg                                       >  2:4494084/1‑93 (MQ=255)
                                                         gcAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGgcgc                                   >  1:3488907/1‑93 (MQ=255)
                                                          cAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCa                                  <  1:1089282/93‑1 (MQ=255)
                                                            gATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATc                                >  1:1967571/1‑93 (MQ=255)
                                                            gATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATc                                >  1:182430/1‑93 (MQ=255)
                                                            gATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATc                                <  1:1696824/93‑1 (MQ=255)
                                                              tGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGa                              >  1:115676/1‑93 (MQ=255)
                                                                 aaaTCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAg                           >  2:3051115/1‑93 (MQ=255)
                                                                  aaTCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGa                          >  2:404735/1‑93 (MQ=255)
                                                                   aTCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGAc                         <  2:3824265/93‑1 (MQ=255)
                                                                   aTCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGAc                         <  1:4644250/93‑1 (MQ=255)
                                                                    tCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACg                        >  1:3724227/1‑93 (MQ=255)
                                                                    tCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACg                        >  2:2616275/1‑93 (MQ=255)
                                                                      aaCCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGc                      <  1:4537000/93‑1 (MQ=255)
                                                                      aaCCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGc                      >  2:1427861/1‑93 (MQ=255)
                                                                       aCCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCt                     >  1:2434211/1‑93 (MQ=255)
                                                                       aCCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCt                     <  2:4618685/93‑1 (MQ=255)
                                                                          cGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGt                  <  1:2048444/93‑1 (MQ=255)
                                                                            ggcggcACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTa                <  1:470965/93‑1 (MQ=255)
                                                                            ggcggcACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTa                >  2:4726534/1‑93 (MQ=255)
                                                                                gcACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCa            >  2:3694891/1‑93 (MQ=255)
                                                                                gcACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCa            >  1:3384161/1‑93 (MQ=255)
                                                                                  acaTTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCAct          <  1:3802931/93‑1 (MQ=255)
                                                                                  acaTTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCAct          <  1:970504/93‑1 (MQ=255)
                                                                                   caTTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCActg         >  1:769278/1‑93 (MQ=255)
                                                                                    aTTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCActgc        <  1:3407244/93‑1 (MQ=255)
                                                                                     ttAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCActgct       >  1:53159/1‑93 (MQ=255)
                                                                                     ttAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCActgct       >  2:1682248/1‑93 (MQ=255)
                                                                                          aCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACa  <  1:2763909/93‑1 (MQ=255)
                                                                                          |                                                                                            
CAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACA  >  NC_000913/4246098‑4246280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACAT  >  NC_000913/4246096‑4246281
                                                                                            |                                                                                             
AACAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGC                                                                                              >  SRR4302544.1703644/1‑94 (MQ=60)
AACAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGC                                                                                              >  SRR4302544.3982952/1‑94 (MQ=60)
   AATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGG                                                                                           >  SRR4302544.3408667/1‑94 (MQ=60)
   AATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGG                                                                                           >  SRR4302544.4012628/1‑94 (MQ=60)
   AATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGG                                                                                           <  SRR4302544.2536554/94‑1 (MQ=60)
    ATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGG                                                                                          <  SRR4302544.2174909/94‑1 (MQ=60)
    ATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGG                                                                                          >  SRR4302544.457848/1‑94 (MQ=60)
    ATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGG                                                                                          >  SRR4302544.3715432/1‑94 (MQ=60)
    ATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGG                                                                                          <  SRR4302544.3573677/94‑1 (MQ=60)
    ATATGTATGACTATACCAACGAATCCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGG                                                                                          <  SRR4302544.2867662/94‑1 (MQ=60)
     TATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGT                                                                                         <  SRR4302544.4404594/94‑1 (MQ=60)
     TATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGT                                                                                         >  SRR4302544.713770/1‑94 (MQ=60)
      ATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTG                                                                                        >  SRR4302544.2942334/1‑94 (MQ=60)
       TTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGT                                                                                       <  SRR4302544.2243337/94‑1 (MQ=60)
       TTTATGACGATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCACCCCGGGCGGCACATTAGAGCTGGGTGT                                                                                       <  SRR4302544.2243338/94‑1 (MQ=60)
          ATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGA                                                                                    >  SRR4302544.63885/1‑94 (MQ=60)
           TGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGAC                                                                                   >  SRR4302544.4735819/1‑94 (MQ=60)
            GACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACT                                                                                  >  SRR4302544.3543981/1‑94 (MQ=60)
            GACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACT                                                                                  >  SRR4302544.3359983/1‑94 (MQ=60)
            GACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACT                                                                                  >  SRR4302544.999605/1‑94 (MQ=60)
            GACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCCGGCGGCACATTAGAGCTGGGTGTCGACT                                                                                  >  SRR4302544.815564/1‑94 (MQ=60)
              CTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTAC                                                                                <  SRR4302544.3791504/94‑1 (MQ=60)
              CTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTAC                                                                                >  SRR4302544.4297483/1‑94 (MQ=60)
              CTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTAC                                                                                >  SRR4302544.4367429/1‑94 (MQ=60)
               TATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCACCCCGGGCGGCCCATTAGAGCTGGGTGTCGACTACG                                                                               >  SRR4302544.714018/1‑94 (MQ=60)
               TATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACG                                                                               >  SRR4302544.4425098/1‑94 (MQ=60)
                 TACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGT                                                                             <  SRR4302544.3933670/94‑1 (MQ=60)
                 TACCAACGAAACCGCGAACGACGTTTTCGATGGGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGT                                                                             <  SRR4302544.1602448/94‑1 (MQ=60)
                  ACCAACGAAACCGCGAACGACTTTTTCGATGGGCGTTTAGCTCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTC                                                                            <  SRR4302544.52694/94‑1 (MQ=60)
                  ACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTC                                                                            <  SRR4302544.3529103/94‑1 (MQ=60)
                  ACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTC                                                                            <  SRR4302544.553818/94‑1 (MQ=60)
                  ACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTC                                                                            >  SRR4302544.3669777/1‑94 (MQ=60)
                   CCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCG                                                                           >  SRR4302544.4762009/1‑94 (MQ=60)
                       CGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCC                                                                       >  SRR4302544.3772111/1‑94 (MQ=60)
                        GAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCA                                                                      <  SRR4302544.1676789/94‑1 (MQ=60)
                         AAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAA                                                                     >  SRR4302544.3448728/1‑94 (MQ=60)
                         AAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAA                                                                     >  SRR4302544.3177221/1‑94 (MQ=60)
                            CCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTT                                                                  >  SRR4302544.2363693/1‑94 (MQ=60)
                             CGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTG                                                                 >  SRR4302544.1539200/1‑94 (MQ=60)
                               CGAACGGCGGTTTGGGTGTGAGTTTAGCGCAGATGGAAATCAACCCGGGCGGCCCATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCG                                                               <  SRR4302544.3168040/94‑1 (MQ=60)
                               CGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCG                                                               >  SRR4302544.3310973/1‑94 (MQ=60)
                                GAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGT                                                              >  SRR4302544.694878/1‑94 (MQ=60)
                                 GACGACGTTTTCGATGTGCGTTTAGCCCATATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTG                                                             <  SRR4302544.3211711/94‑1 (MQ=60)
                                 AACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTG                                                             >  SRR4302544.1641687/1‑94 (MQ=60)
                                 AACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTG                                                             <  SRR4302544.3211710/94‑1 (MQ=60)
                                 AACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTG                                                             <  SRR4302544.1312644/94‑1 (MQ=60)
                                  ACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGA                                                            <  SRR4302544.1317818/94‑1 (MQ=60)
                                  ACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGA                                                            >  SRR4302544.2245660/1‑94 (MQ=60)
                                    GACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATA                                                          >  SRR4302544.3154078/1‑94 (MQ=60)
                                    GACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATA                                                          <  SRR4302544.4643739/94‑1 (MQ=60)
                                     ACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAA                                                         <  SRR4302544.3615001/94‑1 (MQ=60)
                                     ACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAA                                                         <  SRR4302544.2391416/94‑1 (MQ=60)
                                      CGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAAC                                                        <  SRR4302544.1704647/94‑1 (MQ=60)
                                      CGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAAC                                                        <  SRR4302544.1691697/94‑1 (MQ=60)
                                      CGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAAC                                                        <  SRR4302544.900453/94‑1 (MQ=60)
                                       GTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACT                                                       >  SRR4302544.352569/1‑94 (MQ=60)
                                       GTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACT                                                       >  SRR4302544.4519317/1‑94 (MQ=60)
                                        TTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGTGGCACATTAGAGCTGGGTGTCGACTACGGCCGTGCCAACTTGACTGATAACTA                                                      >  SRR4302544.3609316/1‑94 (MQ=60)
                                        TTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTA                                                      <  SRR4302544.759998/94‑1 (MQ=60)
                                         TTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTAT                                                     <  SRR4302544.3280644/94‑1 (MQ=60)
                                            CGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGAGTACGGTCGTGCCAACTTGCGTGATAACTATCGT                                                  >  SRR4302544.1136845/1‑94 (MQ=60)
                                             GATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTC                                                 >  SRR4302544.557690/1‑94 (MQ=60)
                                              ATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCT                                                <  SRR4302544.2527434/94‑1 (MQ=60)
                                              ATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCT                                                <  SRR4302544.1550443/94‑1 (MQ=60)
                                              ATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCT                                                <  SRR4302544.2045394/94‑1 (MQ=60)
                                               TGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTG                                               >  SRR4302544.4368569/1‑94 (MQ=60)
                                               TGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTG                                               <  SRR4302544.3221646/94‑1 (MQ=60)
                                                GTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGG                                              >  SRR4302544.3129048/1‑94 (MQ=60)
                                                GTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGG                                              <  SRR4302544.4054720/94‑1 (MQ=60)
                                                 TGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGT                                             <  SRR4302544.2514482/94‑1 (MQ=60)
                                                 TGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGT                                             <  SRR4302544.488303/94‑1 (MQ=60)
                                                 TGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGT                                             <  SRR4302544.2992570/94‑1 (MQ=60)
                                                  GCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTT                                            >  SRR4302544.2840794/1‑94 (MQ=60)
                                                   CGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTG                                           <  SRR4302544.2877256/94‑1 (MQ=60)
                                                   CGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTG                                           <  SRR4302544.80616/94‑1 (MQ=60)
                                                   CGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTG                                           <  SRR4302544.80617/94‑1 (MQ=60)
                                                   CGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTG                                           <  SRR4302544.1187654/94‑1 (MQ=60)
                                                     TTTAGCGCAGATGGAAATCAACCCGGGCGGCATATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGAT                                         <  SRR4302544.3208973/94‑1 (MQ=60)
                                                     TTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGAT                                         <  SRR4302544.2366419/94‑1 (MQ=60)
                                                      TTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATG                                        <  SRR4302544.4741610/94‑1 (MQ=60)
                                                      TTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATG                                        <  SRR4302544.4020000/94‑1 (MQ=60)
                                                       TAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGTGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGG                                       <  SRR4302544.3093925/94‑1 (MQ=60)
                                                        AGCGCAGATGGAAATCAGCCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGC                                      <  SRR4302544.4103518/94‑1 (MQ=60)
                                                          CGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGC                                    >  SRR4302544.1704065/1‑94 (MQ=60)
                                                          CGCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGC                                    >  SRR4302544.3522168/1‑94 (MQ=60)
                                                           GCAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCA                                   <  SRR4302544.3418267/94‑1 (MQ=60)
                                                            CAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCAT                                  <  SRR4302544.1100112/94‑1 (MQ=60)
                                                            CAGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGGCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCAT                                  >  SRR4302544.790863/1‑94 (MQ=60)
                                                             AGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATC                                 >  SRR4302544.1986679/1‑94 (MQ=60)
                                                             AGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATC                                 >  SRR4302544.184234/1‑94 (MQ=60)
                                                             AGATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATC                                 >  SRR4302544.4479192/1‑94 (MQ=60)
                                                              GATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCG                                <  SRR4302544.1713568/94‑1 (MQ=60)
                                                               ATGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGA                               >  SRR4302544.116731/1‑94 (MQ=60)
                                                                TGGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAA                              <  SRR4302544.4011070/94‑1 (MQ=60)
                                                                 GGAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAA                             >  SRR4302544.439025/1‑94 (MQ=60)
                                                                  GAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAG                            <  SRR4302544.2796034/94‑1 (MQ=60)
                                                                  GAAATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAG                            <  SRR4302544.2392958/94‑1 (MQ=60)
                                                                     ATCAACCGGGGCGGCACATTAGAACTGGGTGTAGTCTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACG                         <  SRR4302544.4406198/94‑1 (MQ=60)
                                                                     ATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACG                         >  SRR4302544.3761264/1‑94 (MQ=60)
                                                                     ATCAACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACG                         <  SRR4302544.4690239/94‑1 (MQ=60)
                                                                        CACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGAGTGATAACTATCGTCCTGTTGATGGCGCATCGAAAGACGctt                      >  SRR4302544.2457413/1‑91 (MQ=60)
                                                                        AACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCT                      >  SRR4302544.2457412/1‑94 (MQ=60)
                                                                        AACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCT                      <  SRR4302544.4582360/94‑1 (MQ=60)
                                                                         ACCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTG                     >  SRR4302544.334478/1‑94 (MQ=60)
                                                                          CCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGG                    >  SRR4302544.3922712/1‑94 (MQ=60)
                                                                          CCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGG                    >  SRR4302544.1606684/1‑94 (MQ=60)
                                                                          CCCGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGG                    <  SRR4302544.239171/94‑1 (MQ=60)
                                                                            CGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTT                  >  SRR4302544.25627/1‑94 (MQ=60)
                                                                            CGGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTT                  <  SRR4302544.2068178/94‑1 (MQ=60)
                                                                             GGGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTA                 <  SRR4302544.2385612/94‑1 (MQ=60)
                                                                             GGGCGGCACATTAGAACTGGGTGTCGACTACGGCCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGGGCATCGAAAGACGGCTGGTTA                 >  SRR4302544.4289721/1‑94 (MQ=60)
                                                                              GGCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTAT                <  SRR4302544.475568/94‑1 (MQ=60)
                                                                               GCGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATT               <  SRR4302544.1777018/94‑1 (MQ=60)
                                                                                CGGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTC              >  SRR4302544.3772042/1‑94 (MQ=60)
                                                                                 GGCACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCA             >  SRR4302544.3416493/1‑94 (MQ=60)
                                                                                    ACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTG          <  SRR4302544.980020/94‑1 (MQ=60)
                                                                                    ACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTG          <  SRR4302544.3840609/94‑1 (MQ=60)
                                                                                    ACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTG          >  SRR4302544.776768/1‑94 (MQ=60)
                                                                                    ACATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTG          <  SRR4302544.326911/94‑1 (MQ=60)
                                                                                     CATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGC         >  SRR4302544.1941401/1‑94 (MQ=60)
                                                                                      ATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCT        >  SRR4302544.53701/1‑94 (MQ=60)
                                                                                      ATTAGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCT        <  SRR4302544.3439819/94‑1 (MQ=60)
                                                                                         AGAGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAA     >  SRR4302544.732696/1‑94 (MQ=60)
                                                                                           AGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACA   >  SRR4302544.4087279/1‑94 (MQ=60)
                                                                                           AGCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACA   >  SRR4302544.268292/1‑94 (MQ=60)
                                                                                            GCTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACAT  <  SRR4302544.3324311/94‑1 (MQ=60)
                                                                                            ACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACAT  <  SRR4302544.2790495/94‑1 (MQ=60)
                                                                                            |                                                                                             
AACAATATTTATGACTATACCAACGAAACCGCGAACGACGTTTTCGATGTGCGTTTAGCGCAGATGGAAATCAACCCGGGCGGCACATTAGAACTGGGTGTCGACTACGGTCGTGCCAACTTGCGTGATAACTATCGTCTGGTTGATGGCGCATCGAAAGACGGCTGGTTATTCACTGCTGAACAT  >  NC_000913/4246096‑4246281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑