Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,250,949 A→G I119V (ATT→GTT)  ubiA → 4‑hydroxybenzoate‑octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,250,9490AG100.0% 564.1 / NA 171I119V (ATT→GTT) ubiA4‑hydroxybenzoate‑octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (87/84);  total (87/84)

Only 100 of 176 total aligned reads displayed.

GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038
                                                                                          |                                                                                         
ggcgCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGtt                                                                                         <  2:2457359/93‑1 (MQ=255)
 gcgcGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTc                                                                                        <  1:1037541/93‑1 (MQ=255)
 gcgcGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTc                                                                                        >  2:1733589/1‑93 (MQ=255)
    cgGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgt                                                                                     <  2:862122/93‑1 (MQ=255)
     ggTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTTACGCTGAATACGATGACCGTTCtgtt                                                                                    <  2:2741221/93‑1 (MQ=255)
      gTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTTAATACGATGACCGTTCtgttg                                                                                   <  2:2773037/93‑1 (MQ=255)
      gTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttg                                                                                   >  1:96896/1‑93 (MQ=255)
       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                  >  1:3730154/1‑93 (MQ=255)
       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                  <  1:903803/93‑1 (MQ=255)
       tAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgttgt                                                                                  <  2:2115479/93‑1 (MQ=255)
          cAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGa                                                                               <  2:1454977/93‑1 (MQ=255)
           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTTGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                              >  2:2043062/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  2:3328468/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             <  1:2060014/93‑1 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  1:167114/93‑1 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          >  2:2645312/1‑93 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  1:3712161/93‑1 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          <  1:3712160/93‑1 (MQ=255)
                 agagTCGCGCGCGCCATGTGGCGTGCTGGTACTGGTTTCGTTTTTGCTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgc                                                                        <  1:1515959/93‑1 (MQ=255)
                   agGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCgcgc                                                                      <  2:3487638/93‑1 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     >  1:738878/1‑93 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     >  1:3491728/1‑93 (MQ=255)
                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    <  2:4080456/93‑1 (MQ=255)
                     gcgcgcgcccTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    <  2:4080457/93‑1 (MQ=255)
                      cgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAg                                                                   <  2:3186346/93‑1 (MQ=255)
                      cgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAg                                                                   <  1:2651287/93‑1 (MQ=255)
                        cgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAgcg                                                                 <  2:1543939/93‑1 (MQ=255)
                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              <  2:888551/93‑1 (MQ=255)
                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              <  2:4303030/93‑1 (MQ=255)
                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              <  2:4345126/93‑1 (MQ=255)
                             gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGc                                                            <  1:2427146/93‑1 (MQ=255)
                             gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGGGCTGACGCTGAATACGATGCCCGTTCTGTTGTCGATTGCCGCGCTAGCGATggg                                                            >  2:229637/1‑92 (MQ=255)
                              cTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCg                                                           >  2:2156249/1‑93 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCgg                                                          >  2:3562134/1‑92 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCgg                                                          >  1:2662820/1‑92 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                          >  2:1954384/1‑93 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                          >  1:4098437/1‑93 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         <  1:3250777/93‑1 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  2:2309234/1‑93 (MQ=255)
                                 tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGCTTGCCGCGCTAGCGCTGGCGTgg                                                        >  1:3350167/1‑93 (MQ=255)
                                 tttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTgg                                                        >  2:596082/1‑93 (MQ=255)
                                   tGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgt                                                      >  1:2557838/1‑93 (MQ=255)
                                   tGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgt                                                      <  1:4637380/93‑1 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     <  1:3356847/93‑1 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     >  1:2183641/1‑93 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     >  2:461622/1‑93 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     <  1:1420027/93‑1 (MQ=255)
                                    gTCGGGCTGGTACTTATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGTCCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     <  2:2909439/93‑1 (MQ=255)
                                      cGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTa                                                   <  2:2963079/93‑1 (MQ=255)
                                         gCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAccc                                                >  2:4548160/1‑93 (MQ=255)
                                          cTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCg                                               >  1:3064563/1‑93 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              <  1:523468/93‑1 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              >  2:549013/1‑93 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              <  1:1388552/93‑1 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              <  2:1594414/93‑1 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGCGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGCGTACCCGtttt                                           >  2:3772106/1‑92 (MQ=255)
                                                cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATg                                         <  1:2108980/93‑1 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:1648317/1‑93 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:2697299/1‑93 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:2471951/1‑93 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        <  1:3819193/93‑1 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  2:4427282/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGCTGACCGTTCTCTTGTCTATTGCCGCGCTCGCGCTGGCGTGCGTATACACGTCTATGag                                       >  1:4234835/1‑92 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       >  1:682214/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       <  2:2302394/93‑1 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       >  1:4234834/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTAGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       <  2:3203900/93‑1 (MQ=255)
                                                    tttCGTTTTGACCGGTGCTGACGCTGAATACGATGCCCATTCTGTTGTCGCTTGCCCCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     <  2:1583814/93‑1 (MQ=255)
                                                     ttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCg                                    >  2:2350069/1‑93 (MQ=255)
                                                      tCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCgg                                   <  2:3574523/93‑1 (MQ=255)
                                                          ttttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGtata                               <  1:1364158/93‑1 (MQ=255)
                                                            ttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAcc                             >  2:1327269/1‑93 (MQ=255)
                                                            ttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAATGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAcc                             <  1:2997657/93‑1 (MQ=255)
                                                             tACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAccc                            >  1:2651715/1‑93 (MQ=255)
                                                             tACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAccc                            >  1:2298247/1‑93 (MQ=255)
                                                                 ggTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCt                        >  2:2682859/1‑93 (MQ=255)
                                                                 ggTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCt                        <  1:1147382/93‑1 (MQ=255)
                                                                 ggTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCt                        <  1:717554/93‑1 (MQ=255)
                                                                    gccgACGCTCAATACTACGACCTTTCTGTTGCCGATTGCCGTGCTCGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAcc                     <  2:3994858/90‑1 (MQ=255)
                                                                    gCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAcc                     <  1:110978/93‑1 (MQ=255)
                                                                       gACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCa                  <  1:3908086/93‑1 (MQ=255)
                                                                        aCGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCaa                 <  1:4175192/93‑1 (MQ=255)
                                                                         cGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAg                >  1:842039/1‑93 (MQ=255)
                                                                         cGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAg                >  1:4529437/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt               >  2:1111320/1‑93 (MQ=255)
                                                                            tGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtgg             >  1:4397965/1‑93 (MQ=255)
                                                                             gAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt            >  1:1313345/1‑93 (MQ=255)
                                                                             gAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt            >  2:3069637/1‑93 (MQ=255)
                                                                             gAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt            >  1:2164147/1‑93 (MQ=255)
                                                                              aaTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggtg           <  1:2968782/93‑1 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt         >  2:16689/1‑93 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt         <  1:3145155/93‑1 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt         >  1:2251171/1‑93 (MQ=255)
                                                                                 aCGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTg        <  1:2874350/93‑1 (MQ=255)
                                                                                 aCGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTg        >  1:2627117/1‑93 (MQ=255)
                                                                                    aTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgc     >  2:66659/1‑93 (MQ=255)
                                                                                     tGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGTgcg    >  2:2878949/1‑93 (MQ=255)
                                                                                     tGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcg    >  1:4002495/1‑93 (MQ=255)
                                                                                      gACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcgc   <  2:4092093/93‑1 (MQ=255)
                                                                                      gACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcgc   >  2:2912813/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCG                                                                                               >  SRR4302544.2133044/1‑94 (MQ=60)
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCG                                                                                               >  SRR4302544.1951051/1‑94 (MQ=60)
 GCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGT                                                                                              <  SRR4302544.3617007/94‑1 (MQ=60)
 CCGGCGCGGTAATAGATACAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACGGGTGCTGACGCTGAATACGATGACCGT                                                                                              <  SRR4302544.3617008/94‑1 (MQ=60)
  CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTT                                                                                             <  SRR4302544.50420/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302544.1047854/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302544.295223/94‑1 (MQ=60)
        GGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTG                                                                                       >  SRR4302544.97782/1‑94 (MQ=60)
         GTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGT                                                                                      >  SRR4302544.3767240/1‑94 (MQ=60)
         GTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGGACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGT                                                                                      >  SRR4302544.2155526/1‑94 (MQ=60)
          TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTC                                                                                     <  SRR4302544.912749/94‑1 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTTGTGCTGGTACTGATTTCGTTTTTTCTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  >  SRR4302544.3388474/1‑94 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 <  SRR4302544.4166492/94‑1 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 >  SRR4302544.2326739/1‑94 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCCGTTTGTCGTTCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGGTCTGTTGTCGATT                                                                                 >  SRR4302544.4244041/1‑94 (MQ=60)
               GAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTG                                                                                >  SRR4302544.1211194/1‑94 (MQ=60)
               GAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTG                                                                                <  SRR4302544.2079826/94‑1 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             <  SRR4302544.3749086/94‑1 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             <  SRR4302544.3749085/94‑1 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             <  SRR4302544.3135893/94‑1 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             <  SRR4302544.168760/94‑1 (MQ=60)
                    AGAGTCGCGCGCGCCATGTGGCGTGCTGGTACTGGTTTCGTTTTTGCTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                           <  SRR4302544.1530993/94‑1 (MQ=60)
                    AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                           <  SRR4302544.1530994/94‑1 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302544.3525020/1‑94 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         <  SRR4302544.2748697/94‑1 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302544.746109/1‑94 (MQ=60)
                       GGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTA                                                                        <  SRR4302544.918526/94‑1 (MQ=60)
                        GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAG                                                                       <  SRR4302544.2086076/94‑1 (MQ=60)
                        GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAG                                                                       <  SRR4302544.4525530/94‑1 (MQ=60)
                         CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGC                                                                      <  SRR4302544.2676711/94‑1 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                  <  SRR4302544.3463577/94‑1 (MQ=60)
                                GCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCG                                                               <  SRR4302544.2450245/94‑1 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302544.4139221/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGG                                                              >  SRR4302544.2688348/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTCCCGCGCTAGCGCTGGGGT                                                              >  SRR4302544.4139220/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTGCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGG                                                              >  SRR4302544.2655581/1‑94 (MQ=60)
                                  TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                             >  SRR4302544.57664/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGCTTGCCGCGCTAGCGCTGGCGTGG                                                            >  SRR4302544.3382133/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            >  SRR4302544.2712297/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            <  SRR4302544.3281875/94‑1 (MQ=60)
                                    TTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGG                                                           >  SRR4302544.2294598/1‑94 (MQ=60)
                                     TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGT                                                          >  SRR4302544.3790924/1‑94 (MQ=60)
                                     TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGT                                                          >  SRR4302544.2582400/1‑94 (MQ=60)
                                      TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                         >  SRR4302544.2204305/1‑94 (MQ=60)
                                      TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                         <  SRR4302544.4683321/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302544.3388879/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302544.1434163/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        >  SRR4302544.4377893/1‑94 (MQ=60)
                                            GCTGGTGCTGTGTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCG                                                   <  SRR4302544.743715/94‑1 (MQ=60)
                                            GCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCG                                                   >  SRR4302544.3093785/1‑94 (MQ=60)
                                            GCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCG                                                   <  SRR4302544.268953/94‑1 (MQ=60)
                                             CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                  >  SRR4302544.1917733/1‑94 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 <  SRR4302544.528645/94‑1 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 <  SRR4302544.1402416/94‑1 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 <  SRR4302544.1402552/94‑1 (MQ=60)
                                                  ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                             <  SRR4302544.2501608/94‑1 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302544.1664581/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302544.2723207/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302544.2495583/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            <  SRR4302544.2129266/94‑1 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGCTGACCGTTCTCTTGTCTATTGCCGCGCTCGCGCTGGCGTGcgtatacacgtctatgag                                           >  SRR4302544.4277999/1‑76 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           <  SRR4302544.3857052/94‑1 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           >  SRR4302544.4277998/1‑94 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           >  SRR4302544.688996/1‑94 (MQ=60)
                                                    TGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAA                                           >  SRR4302544.688997/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                         >  SRR4302544.3269644/1‑94 (MQ=60)
                                                       TTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                        <  SRR4302544.1482278/94‑1 (MQ=60)
                                                             TTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAC                                  <  SRR4302544.1377717/94‑1 (MQ=60)
                                                               TTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCC                                >  SRR4302544.2677142/1‑94 (MQ=60)
                                                               TTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCC                                >  SRR4302544.2319935/1‑94 (MQ=60)
                                                               TTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAATGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCC                                <  SRR4302544.3026398/94‑1 (MQ=60)
                                                                    GGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTA                           <  SRR4302544.1158792/94‑1 (MQ=60)
                                                                    GGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTA                           <  SRR4302544.4670559/94‑1 (MQ=60)
                                                                    GGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTA                           <  SRR4302544.724639/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        <  SRR4302544.112134/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        <  SRR4302544.111993/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        >  SRR4302544.2636512/1‑94 (MQ=60)
                                                                          GACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAA                     <  SRR4302544.2204856/94‑1 (MQ=60)
                                                                          GACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAA                     <  SRR4302544.3946915/94‑1 (MQ=60)
                                                                           ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                    <  SRR4302544.4216550/94‑1 (MQ=60)
                                                                           ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                    >  SRR4302544.850347/1‑94 (MQ=60)
                                                                           ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                    >  SRR4302544.4574754/1‑94 (MQ=60)
                                                                             GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                  >  SRR4302544.76432/1‑94 (MQ=60)
                                                                             GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                  >  SRR4302544.3572266/1‑94 (MQ=60)
                                                                              CTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGG                 >  SRR4302544.4442425/1‑94 (MQ=60)
                                                                               TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT                >  SRR4302544.1326257/1‑94 (MQ=60)
                                                                               TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT                >  SRR4302544.2184692/1‑94 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG               >  SRR4302544.1358593/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              <  SRR4302544.2997253/94‑1 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             >  SRR4302544.3049843/1‑94 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             >  SRR4302544.2272328/1‑94 (MQ=60)
                                                                                   TACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTG            <  SRR4302544.3175055/94‑1 (MQ=60)
                                                                                   TACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTG            >  SRR4302544.2652306/1‑94 (MQ=60)
                                                                                    ACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGG           <  SRR4302544.2902093/94‑1 (MQ=60)
                                                                                       ATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCG        >  SRR4302544.4042328/1‑94 (MQ=60)
                                                                                        TGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC       <  SRR4302544.3757387/94‑1 (MQ=60)
                                                                                        TGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC       >  SRR4302544.3873746/1‑94 (MQ=60)
                                                                                          ACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGG     >  SRR4302544.3282042/1‑94 (MQ=60)
                                                                                           CCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGC    >  SRR4302544.2693561/1‑94 (MQ=60)
                                                                                            CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG   >  SRR4302544.960047/1‑94 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  SRR4302544.813548/1‑94 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  SRR4302544.92494/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑