Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,250,949 A→G I119V (ATT→GTT)  ubiA → 4‑hydroxybenzoate‑octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,250,9490AG99.2% 837.9 / NA 256I119V (ATT→GTT) ubiA4‑hydroxybenzoate‑octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (127/127);  minor base T (0/2);  total (127/129)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038
                                                                                          |                                                                                         
ggcgCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTAATGGTGCTGACGCTGAATACGATGACCGtt                                                                                         <  1:3033016/93‑1 (MQ=255)
 gcgcGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTc                                                                                        <  1:1375349/93‑1 (MQ=255)
  cgcgGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCt                                                                                       <  2:2873391/93‑1 (MQ=255)
     ggTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCtgtt                                                                                    <  2:565280/93‑1 (MQ=255)
        aaCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTc                                                                                 >  2:1019667/1‑93 (MQ=255)
         aCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCg                                                                                >  1:754234/1‑93 (MQ=255)
         aCAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCg                                                                                >  2:4059737/1‑93 (MQ=255)
          cAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGa                                                                               <  2:3282142/93‑1 (MQ=255)
          cAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGa                                                                               <  1:286212/93‑1 (MQ=255)
          aagagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGa                                                                               <  2:1720236/92‑1 (MQ=255)
           agagAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAt                                                                              <  1:5264528/93‑1 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             <  1:4585075/93‑1 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             <  2:2240113/93‑1 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  2:6656249/1‑93 (MQ=255)
            gagaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATCTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAtt                                                                             >  2:516363/1‑93 (MQ=255)
              gaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGc                                                                           >  2:2136459/1‑93 (MQ=255)
              gaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGc                                                                           >  2:3293088/1‑93 (MQ=255)
              gaAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGc                                                                           >  1:1290882/1‑93 (MQ=255)
               aaaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGcc                                                                          >  2:2987165/1‑93 (MQ=255)
                aaGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcg                                                                         >  1:6482091/1‑93 (MQ=255)
                  gagGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCcgcg                                                                       >  2:1217009/1‑93 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     >  1:6188277/1‑93 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     >  1:6607980/1‑93 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     <  2:659304/93‑1 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     <  1:3934586/93‑1 (MQ=255)
                    ggCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCt                                                                     >  1:227884/1‑93 (MQ=255)
                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    >  2:4309661/1‑93 (MQ=255)
                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    >  2:5324529/1‑93 (MQ=255)
                     gcgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTa                                                                    <  1:5839748/93‑1 (MQ=255)
                      cgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAg                                                                   <  1:949965/93‑1 (MQ=255)
                      cgcgcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAg                                                                   <  2:811055/93‑1 (MQ=255)
                         gcgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAgcgc                                                                <  2:3125332/93‑1 (MQ=255)
                          cgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCt                                                               <  2:1727410/93‑1 (MQ=255)
                          cgcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCt                                                               <  1:2667649/93‑1 (MQ=255)
                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              <  1:3783058/93‑1 (MQ=255)
                           gcgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTg                                                              <  1:4380690/93‑1 (MQ=255)
                            cgcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTgg                                                             <  2:4992408/93‑1 (MQ=255)
                             gcTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTCACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGc                                                            >  2:3563316/1‑93 (MQ=255)
                             gcTGTTTGTCGTGCTGGCACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCCGGc                                                            >  1:6326856/1‑93 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                          >  1:1176767/1‑93 (MQ=255)
                               tGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGt                                                          >  1:1805316/1‑93 (MQ=255)
                                ttttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         <  2:3232151/92‑1 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         <  2:6371657/93‑1 (MQ=255)
                                gTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTg                                                         >  1:4904916/1‑93 (MQ=255)
                                  ttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTggg                                                       <  1:3159917/93‑1 (MQ=255)
                                  ttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTggg                                                       <  1:2263752/93‑1 (MQ=255)
                                  ttGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTggg                                                       <  2:5465032/93‑1 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     >  1:1590382/1‑93 (MQ=255)
                                    gTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtg                                                     <  1:3626280/93‑1 (MQ=255)
                                     tCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGgtgt                                                    >  1:2453049/1‑93 (MQ=255)
                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                  >  1:1126278/1‑93 (MQ=255)
                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                  >  1:339639/1‑93 (MQ=255)
                                       gTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAc                                                  <  1:1635301/93‑1 (MQ=255)
                                          cTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCg                                               >  2:6273090/1‑93 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              <  2:4393824/93‑1 (MQ=255)
                                           tGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGt                                              >  1:3090021/1‑93 (MQ=255)
                                             gTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGttt                                            <  1:4834428/93‑1 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                           <  2:5313984/93‑1 (MQ=255)
                                              tACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTa                                           <  2:6240457/93‑1 (MQ=255)
                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          <  2:5670814/93‑1 (MQ=255)
                                               aCTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAt                                          <  2:2608384/93‑1 (MQ=255)
                                                cTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATg                                         >  2:4175047/1‑93 (MQ=255)
                                                 tGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGa                                        >  1:3372614/1‑93 (MQ=255)
                                                  gATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGaa                                       <  1:1494841/93‑1 (MQ=255)
                                                   aTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAg                                      >  1:1887974/1‑93 (MQ=255)
                                                    tttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGc                                     >  1:4950994/1‑93 (MQ=255)
                                                     ttCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCg                                    <  1:5699348/93‑1 (MQ=255)
                                                     ttCGTTTTTACTGGTGCTGACGCTGAATACAATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCg                                    >  1:5118502/1‑93 (MQ=255)
                                                       cGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                  <  1:6466418/93‑1 (MQ=255)
                                                       cGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                  <  2:1137363/93‑1 (MQ=255)
                                                       cGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                  >  2:1304545/1‑93 (MQ=255)
                                                       cGTTTTTACCGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGt                                  <  1:2093102/93‑1 (MQ=255)
                                                         tttttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGtat                                >  1:3326174/1‑93 (MQ=255)
                                                          ttttACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGtata                               >  2:2670250/1‑93 (MQ=255)
                                                             tACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATAccc                            >  1:4120401/1‑93 (MQ=255)
                                                               cTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAt                          <  1:5574055/93‑1 (MQ=255)
                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATc                         >  2:2277932/1‑93 (MQ=255)
                                                                tGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATc                         >  2:1354703/1‑93 (MQ=255)
                                                                  gTGCTCACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGGGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTa                       <  1:4236016/93‑1 (MQ=255)
                                                                   tGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAc                      <  2:1861124/93‑1 (MQ=255)
                                                                    gCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAcc                     <  1:4303873/93‑1 (MQ=255)
                                                                     cTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCg                    >  2:1961042/1‑93 (MQ=255)
                                                                       gACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCa                  >  1:2853075/1‑93 (MQ=255)
                                                                         cGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAg                >  1:856657/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt               >  2:3924856/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt               <  2:4110358/93‑1 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgt               >  2:4789639/1‑93 (MQ=255)
                                                                          gCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCAGATTATGAAGAGGTATACCCATCTACCGCAAgg               >  2:363923/1‑92 (MQ=255)
                                                                           cTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtg              <  2:2449709/93‑1 (MQ=255)
                                                                            tGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtgg             <  2:3401721/93‑1 (MQ=255)
                                                                            tGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtgg             <  2:2295058/93‑1 (MQ=255)
                                                                             gAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt            <  1:3568993/93‑1 (MQ=255)
                                                                             gAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggt            >  2:1628234/1‑93 (MQ=255)
                                                                              aaTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAgtggtg           <  1:2483710/93‑1 (MQ=255)
                                                                               aTACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGc          >  1:5253410/1‑93 (MQ=255)
                                                                                tACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCt         >  2:5177451/1‑93 (MQ=255)
                                                                                  cGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTgg       >  1:3698963/1‑93 (MQ=255)
                                                                                   gATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTggg      >  1:4664763/1‑93 (MQ=255)
                                                                                    aTGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgc     >  1:6137426/1‑93 (MQ=255)
                                                                                     tGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGGGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGgcg    >  2:4695047/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG  >  NC_000913/4250859‑4251038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCG                                                                                               >  SRR4302536.3110683/1‑94 (MQ=60)
 GCGGCTCGGCAACAGAGAAAGAGGCGCGCGCTGTGTTTGCCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGATGAATTCGACGACCGT                                                                                              <  SRR4302536.3648799/94‑1 (MQ=60)
 GCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGT                                                                                              <  SRR4302536.3826292/94‑1 (MQ=60)
 GCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGT                                                                                              <  SRR4302536.3948789/94‑1 (MQ=60)
  CGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTT                                                                                             >  SRR4302536.5965513/1‑94 (MQ=60)
   GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTC                                                                                            >  SRR4302536.6272051/1‑94 (MQ=60)
   GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTC                                                                                            >  SRR4302536.2946059/1‑94 (MQ=60)
   GGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTAATGGTGCTGACGCTGAATACGATGACCGTTC                                                                                            <  SRR4302536.3122148/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302536.1416318/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302536.3193890/94‑1 (MQ=60)
    GCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCT                                                                                           <  SRR4302536.4074176/94‑1 (MQ=60)
       CGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTT                                                                                        >  SRR4302536.2773901/1‑94 (MQ=60)
         GTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGT                                                                                      <  SRR4302536.3760361/94‑1 (MQ=60)
          TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGCCCGTTCTGTTGTC                                                                                     <  SRR4302536.4263251/94‑1 (MQ=60)
          TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTC                                                                                     <  SRR4302536.3478529/94‑1 (MQ=60)
          TAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATAAGATGATCGTTCTGTTGTC                                                                                     >  SRR4302536.1467420/1‑94 (MQ=60)
           AACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCG                                                                                    >  SRR4302536.1021197/1‑94 (MQ=60)
           AACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCG                                                                                    >  SRR4302536.776359/1‑94 (MQ=60)
           AACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGCGCTGGTTCTGGTTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGCTCTGTTGTCG                                                                                    >  SRR4302536.4635108/1‑94 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  >  SRR4302536.1661385/1‑94 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  <  SRR4302536.5033121/94‑1 (MQ=60)
             CAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGAT                                                                                  <  SRR4302536.293826/94‑1 (MQ=60)
              AGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATT                                                                                 <  SRR4302536.5421432/94‑1 (MQ=60)
               GAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTG                                                                                >  SRR4302536.650951/1‑94 (MQ=60)
               GAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTG                                                                                <  SRR4302536.4722698/94‑1 (MQ=60)
                AGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGC                                                                               >  SRR4302536.1329479/1‑94 (MQ=60)
                AGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGC                                                                               >  SRR4302536.3152922/1‑94 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             >  SRR4302536.125326/1‑94 (MQ=60)
                  AAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCG                                                                             >  SRR4302536.6675432/1‑94 (MQ=60)
                    AGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCG                                                                           <  SRR4302536.5108028/94‑1 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302536.233940/1‑94 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302536.6372584/1‑94 (MQ=60)
                      AGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCT                                                                         >  SRR4302536.6804478/1‑94 (MQ=60)
                       GGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTA                                                                        <  SRR4302536.4051477/94‑1 (MQ=60)
                       GGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTA                                                                        <  SRR4302536.962831/94‑1 (MQ=60)
                        GCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAG                                                                       <  SRR4302536.6013740/94‑1 (MQ=60)
                         CGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGC                                                                      <  SRR4302536.978043/94‑1 (MQ=60)
                           CGCGCGCTGTTTGTCGTGCTGGGACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTTGATTGCCGCGCTAGCGC                                                                    >  SRR4302536.3561812/1‑94 (MQ=60)
                            GCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCT                                                                   >  SRR4302536.3549132/1‑94 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                  <  SRR4302536.2747440/94‑1 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTG                                                                  <  SRR4302536.177913/94‑1 (MQ=60)
                             CGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTA                                                                  <  SRR4302536.5324132/94‑1 (MQ=60)
                              GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                 <  SRR4302536.4511470/94‑1 (MQ=60)
                              GCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGG                                                                 <  SRR4302536.3895936/94‑1 (MQ=60)
                               CGCTGTTTGTCGTGCTGGTACTGATTCCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCCCTGGC                                                                <  SRR4302536.3010132/94‑1 (MQ=60)
                               CGCTGTTTGTCGTGCTGGCACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCCGGC                                                                >  SRR4302536.6515290/1‑94 (MQ=60)
                                GCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCG                                                               >  SRR4302536.666650/1‑94 (MQ=60)
                                GCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCG                                                               <  SRR4302536.1760785/94‑1 (MQ=60)
                                GCTGTTTGTCGGGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTACCGCGCTAGCGCTGGCG                                                               >  SRR4302536.5261987/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302536.2266106/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302536.1212192/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302536.1859801/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302536.233498/1‑94 (MQ=60)
                                 CTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGT                                                              >  SRR4302536.1382262/1‑94 (MQ=60)
                                  TGTTTGTTGTGCTGCTACTGATTTCGTTTTTACTGGTTCTGACGCTGAATCCGATAACCGTTCTGTTGTCGATTGCCGCGCCAGCGCTGGCGTG                                                             <  SRR4302536.1234424/94‑1 (MQ=60)
                                  TGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTG                                                             >  SRR4302536.5052378/1‑94 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            <  SRR4302536.2141609/94‑1 (MQ=60)
                                   GTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGG                                                            >  SRR4302536.4505609/1‑94 (MQ=60)
                                     TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGT                                                          <  SRR4302536.2332324/94‑1 (MQ=60)
                                     TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGT                                                          <  SRR4302536.3252486/94‑1 (MQ=60)
                                     TTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGG                                                          >  SRR4302536.499971/1‑94 (MQ=60)
                                      TGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTG                                                         >  SRR4302536.1636894/1‑94 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302536.6702953/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        <  SRR4302536.3734732/94‑1 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGT                                                        >  SRR4302536.2527136/1‑94 (MQ=60)
                                       GTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGAGCTGGCGTGGGTGA                                                        >  SRR4302536.1602258/1‑94 (MQ=60)
                                        TCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTA                                                       <  SRR4302536.2181623/94‑1 (MQ=60)
                                         CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                      >  SRR4302536.6616397/1‑94 (MQ=60)
                                         CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                      >  SRR4302536.1160115/1‑94 (MQ=60)
                                         CGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTAC                                                      >  SRR4302536.348630/1‑94 (MQ=60)
                                          GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                     <  SRR4302536.1682917/94‑1 (MQ=60)
                                          GTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACC                                                     >  SRR4302536.2736542/1‑94 (MQ=60)
                                           TGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCC                                                    >  SRR4302536.4747698/1‑94 (MQ=60)
                                           TGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCC                                                    >  SRR4302536.3458087/1‑94 (MQ=60)
                                             CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                  >  SRR4302536.3180365/1‑94 (MQ=60)
                                             CTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGT                                                  >  SRR4302536.3401606/1‑94 (MQ=60)
                                              TGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTT                                                 >  SRR4302536.5760814/1‑94 (MQ=60)
                                                GTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTA                                               <  SRR4302536.4979940/94‑1 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              <  SRR4302536.5316140/94‑1 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              >  SRR4302536.6338241/1‑94 (MQ=60)
                                                 TACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTAT                                              <  SRR4302536.5544792/94‑1 (MQ=60)
                                                  ACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATG                                             <  SRR4302536.6698652/94‑1 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            >  SRR4302536.3472806/1‑94 (MQ=60)
                                                   CTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGA                                            <  SRR4302536.3729256/94‑1 (MQ=60)
                                                     GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                          <  SRR4302536.389356/94‑1 (MQ=60)
                                                     GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                          <  SRR4302536.1538977/94‑1 (MQ=60)
                                                     GATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAG                                          >  SRR4302536.1944764/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                         >  SRR4302536.1242327/1‑94 (MQ=60)
                                                      ATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGC                                         >  SRR4302536.5099743/1‑94 (MQ=60)
                                                       TTTCGTTTTTACTGGTGCTGACGCTGAATACAATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCG                                        >  SRR4302536.5271451/1‑94 (MQ=60)
                                                        TTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGG                                       >  SRR4302536.5092293/1‑94 (MQ=60)
                                                        TTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGG                                       <  SRR4302536.5868810/94‑1 (MQ=60)
                                                        TTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGG                                       >  SRR4302536.3255150/1‑94 (MQ=60)
                                                         TCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGGGTGGGTGTACCCGTTTATGAAGCGGT                                      <  SRR4302536.162976/94‑1 (MQ=60)
                                                         TCGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGT                                      <  SRR4302536.4372479/94‑1 (MQ=60)
                                                          CGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302536.2783232/94‑1 (MQ=60)
                                                          CGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302536.5419472/94‑1 (MQ=60)
                                                          CGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302536.4419449/94‑1 (MQ=60)
                                                          CGTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302536.6659427/94‑1 (MQ=60)
                                                          CGTTTTTACCGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTA                                     <  SRR4302536.2156077/94‑1 (MQ=60)
                                                           GTTTTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                    >  SRR4302536.3424597/1‑94 (MQ=60)
                                                           GTTTTTACTCGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTAT                                    <  SRR4302536.4578151/94‑1 (MQ=60)
                                                             TTTTACTGGTGCTGACGCTGAATACGATGACCCTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGctggggtacccgttttcttaacggaaaac                                  >  SRR4302536.4834157/1‑65 (MQ=60)
                                                              TTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACC                                 <  SRR4302536.4883334/94‑1 (MQ=60)
                                                              TTTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACC                                 >  SRR4302536.6271557/1‑94 (MQ=60)
                                                               TTACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCC                                >  SRR4302536.4242565/1‑94 (MQ=60)
                                                                 ACTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCAT                              <  SRR4302536.3090421/94‑1 (MQ=60)
                                                                  ttggccaTGACGCTGAATCCGATCAGCGTTCTGTTGTCGATTGCCGCGATATCGCTGGCGTGGGTGTACCCGTTTATGAAGTGGTATACCCATC                             <  SRR4302536.2662007/87‑1 (MQ=60)
                                                                  CTGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATC                             <  SRR4302536.5739982/94‑1 (MQ=60)
                                                                   TGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACTCATCT                            >  SRR4302536.6602039/1‑94 (MQ=60)
                                                                   TGGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCT                            <  SRR4302536.4795239/94‑1 (MQ=60)
                                                                    GGTGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTA                           <  SRR4302536.3882853/94‑1 (MQ=60)
                                                                     GTGCTCACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGGGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTAC                          <  SRR4302536.4362107/94‑1 (MQ=60)
                                                                      TGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACC                         <  SRR4302536.1479726/94‑1 (MQ=60)
                                                                      TGCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGGTTATGAAGCGGTATACCCATCTACC                         >  SRR4302536.3474244/1‑94 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        <  SRR4302536.5616180/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        <  SRR4302536.863454/94‑1 (MQ=60)
                                                                       GCTGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCG                        <  SRR4302536.4432575/94‑1 (MQ=60)
                                                                         TGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCA                      >  SRR4302536.2937412/1‑94 (MQ=60)
                                                                         TGACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCA                      <  SRR4302536.388732/94‑1 (MQ=60)
                                                                          GACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAA                     >  SRR4302536.237318/1‑94 (MQ=60)
                                                                           ACGCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAG                    >  SRR4302536.881837/1‑94 (MQ=60)
                                                                             GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCTATCTACCGCAAGTG                  <  SRR4302536.3787718/94‑1 (MQ=60)
                                                                             GCTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTG                  <  SRR4302536.882268/94‑1 (MQ=60)
                                                                              CTGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGG                 >  SRR4302536.1865339/1‑94 (MQ=60)
                                                                               TGAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGT                <  SRR4302536.6492079/94‑1 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG               >  SRR4302536.825771/1‑94 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG               <  SRR4302536.3675694/94‑1 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTG               <  SRR4302536.2505806/94‑1 (MQ=60)
                                                                                GAATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGGGTACCCGTTTATTACGCGGTATACCCATCTATCGCAAGTCGTG               >  SRR4302536.2531068/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              >  SRR4302536.2063838/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              <  SRR4302536.2558648/94‑1 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              >  SRR4302536.732760/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              >  SRR4302536.5409942/1‑94 (MQ=60)
                                                                                 AATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGCTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGC              >  SRR4302536.4212024/1‑94 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             <  SRR4302536.2622151/94‑1 (MQ=60)
                                                                                  ATACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCT             <  SRR4302536.5789960/94‑1 (MQ=60)
                                                                                    ACGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGG           >  SRR4302536.3809572/1‑94 (MQ=60)
                                                                                     CGATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGG          >  SRR4302536.4805013/1‑94 (MQ=60)
                                                                                     CGATGACCGTTCTGTTGGCGATTGCCGCGCTAGCGCTGGCGTGGGTGTGCCCGTTTATGAAGCGGTATACCCATCTACAGCAAGTGGAGCTGGG          >  SRR4302536.300420/1‑94 (MQ=60)
                                                                                     CGAGGACCGTTCTGTTGTCGATTGCCGCGCTAGCTCTGGCGTGGGTGTTCACTTTTATGAAGCGGTATACCCACCTACCGCAAttggttgtgtg          <  SRR4302536.3487287/94‑12 (MQ=59)
                                                                                      GATGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGC         >  SRR4302536.6320330/1‑94 (MQ=60)
                                                                                        TGACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGC       >  SRR4302536.3211086/1‑94 (MQ=60)
                                                                                         GACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCG      <  SRR4302536.1182822/94‑1 (MQ=60)
                                                                                          ACCGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGG     >  SRR4302536.5253013/1‑94 (MQ=60)
                                                                                            CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG   <  SRR4302536.2844780/94‑1 (MQ=60)
                                                                                            CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCG   >  SRR4302536.3582349/1‑94 (MQ=60)
                                                                                            CGTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGGAACCGTTTATGAAGACGTATAACCATCTAGCGCAAGCGGTGCTGGGCACGGGG   >  SRR4302536.3582350/1‑94 (MQ=57)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  <  SRR4302536.6367227/94‑1 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  SRR4302536.5189931/1‑94 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  <  SRR4302536.2742409/94‑1 (MQ=60)
                                                                                             GTTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  <  SRR4302536.2899324/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGCGGCGCGGTAACAGAGAAAGAGGCGCGCGCGCTGTTTGTCGTGCTGGTACTGATTTCGTTTTTACTGGTGCTGACGCTGAATACGATGACCATTCTGTTGTCGATTGCCGCGCTAGCGCTGGCGTGGGTGTACCCGTTTATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGT  >  NC_000913/4250856‑4251042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑