Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,251,088 T→C V165A (GTG→GCG)  ubiA → 4‑hydroxybenzoate‑octaprenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,251,0880TC100.0% 702.3 / NA 220V165A (GTG→GCG) ubiA4‑hydroxybenzoate‑octaprenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/120);  total (100/120)

Only 100 of 234 total aligned reads displayed.

TGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATG  >  NC_000913/4250998‑4251177
                                                                                          |                                                                                         
 gAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGcc                                                                                        <  1:965153/93‑1 (MQ=255)
  aaGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCa                                                                                       <  2:3272819/93‑1 (MQ=255)
   aGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAt                                                                                      >  1:3905476/1‑93 (MQ=255)
   aGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAt                                                                                      <  1:2085309/93‑1 (MQ=255)
   aGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAt                                                                                      <  2:6235867/93‑1 (MQ=255)
    gCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAtt                                                                                     >  2:4602020/1‑93 (MQ=255)
     cGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTg                                                                                    <  1:2417602/93‑1 (MQ=255)
      ggTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGa                                                                                   >  1:2190101/1‑93 (MQ=255)
       gTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAg                                                                                  >  1:2494449/1‑93 (MQ=255)
          taCCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTg                                                                               >  1:5429449/1‑93 (MQ=255)
           aCCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGc                                                                              <  2:3119634/93‑1 (MQ=255)
            cccATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGGGCCCTTGAGGTGCt                                                                             >  2:6107817/1‑93 (MQ=255)
            cccATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCt                                                                             >  1:4195501/1‑93 (MQ=255)
            cccATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCt                                                                             >  1:5364048/1‑93 (MQ=255)
             ccATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTg                                                                            <  2:4778625/93‑1 (MQ=255)
              cATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTgg                                                                           >  1:1454964/1‑93 (MQ=255)
               aTCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGt                                                                          >  1:508848/1‑93 (MQ=255)
                tCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCTATTGAGTTGCTGGtt                                                                         <  1:5000887/93‑1 (MQ=255)
                tCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGtt                                                                         >  1:1685566/1‑93 (MQ=255)
                tCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGtt                                                                         >  2:2376063/1‑93 (MQ=255)
                tCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGACGGCGCCATTGAGTTGCTGGtt                                                                         >  2:4903246/1‑93 (MQ=255)
                  tACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTaa                                                                       <  1:4059737/93‑1 (MQ=255)
                   aCCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAAt                                                                      >  2:2751470/1‑93 (MQ=255)
                    ccGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATg                                                                     >  2:4985260/1‑93 (MQ=255)
                     cGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGt                                                                    <  2:2471421/93‑1 (MQ=255)
                     cGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTACCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGt                                                                    <  2:4691789/93‑1 (MQ=255)
                       cAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTc                                                                  <  2:231160/93‑1 (MQ=255)
                        aaGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTcc                                                                 >  2:2119854/1‑93 (MQ=255)
                         aGTGGTGCTGGGCGCGGCGCTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCt                                                                >  1:6545424/1‑93 (MQ=255)
                          gtggtgCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTc                                                               >  2:3541505/1‑93 (MQ=255)
                            ggtgCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGc                                                             >  2:3640805/1‑93 (MQ=255)
                            ggtgCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGc                                                             <  2:6424376/93‑1 (MQ=255)
                               gCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCaa                                                          <  1:4804415/93‑1 (MQ=255)
                               gCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCaa                                                          >  1:1016290/1‑93 (MQ=255)
                               gCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCaa                                                          <  2:4304092/93‑1 (MQ=255)
                                cTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAat                                                         <  2:5731727/93‑1 (MQ=255)
                                cTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAat                                                         <  1:647383/93‑1 (MQ=255)
                                 tGGTCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAata                                                        <  1:2368255/93‑1 (MQ=255)
                                 tGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAata                                                        <  1:458980/93‑1 (MQ=255)
                                 tGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAata                                                        <  1:4233894/93‑1 (MQ=255)
                                  gggCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAatat                                                       <  1:2987293/93‑1 (MQ=255)
                                  gggCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAatat                                                       <  2:6167695/93‑1 (MQ=255)
                                  gggCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAatat                                                       <  2:6066175/93‑1 (MQ=255)
                                   ggCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATAtt                                                      <  1:5819847/93‑1 (MQ=255)
                                    gcgcGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATtc                                                     <  2:6586711/93‑1 (MQ=255)
                                    gcgcGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATtc                                                     <  1:6110271/93‑1 (MQ=255)
                                     cgcgGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATtct                                                    >  1:3487205/1‑93 (MQ=255)
                                       cggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTctct                                                  >  2:2810019/1‑93 (MQ=255)
                                       cggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTctct                                                  >  1:4572242/1‑93 (MQ=255)
                                       cggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTctct                                                  <  2:3021899/93‑1 (MQ=255)
                                        ggcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTg                                                 <  1:2917669/93‑1 (MQ=255)
                                         gcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTgg                                                <  2:2170022/93‑1 (MQ=255)
                                         gcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTgg                                                <  2:1290882/93‑1 (MQ=255)
                                         gcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTgg                                                <  2:3951378/93‑1 (MQ=255)
                                         gcgTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTgg                                                >  1:3180393/1‑93 (MQ=255)
                                            tttGGCTGGTCGATTCCAATGGCTTTTGTTGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCCATCTTCTCCGGGCg                                             >  1:3750622/1‑93 (MQ=255)
                                                  tGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCt                                       <  2:5183532/93‑1 (MQ=255)
                                                   ggTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCtt                                      <  2:1559031/93‑1 (MQ=255)
                                                   ggTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCtt                                      <  2:3162512/93‑1 (MQ=255)
                                                     tCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTAc                                    >  2:6337435/1‑93 (MQ=255)
                                                      cGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACg                                   >  2:946454/1‑93 (MQ=255)
                                                        aTTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGac                                 <  2:4065970/93‑1 (MQ=255)
                                                        aTTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGac                                 >  1:3885447/1‑93 (MQ=255)
                                                         ttCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGaca                                >  2:2691775/1‑93 (MQ=255)
                                                          tCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGacac                               >  1:4859492/1‑93 (MQ=255)
                                                           ccAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACg                              >  2:5509769/1‑93 (MQ=255)
                                                           ccAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACg                              <  2:2069871/93‑1 (MQ=255)
                                                            cAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACgg                             <  1:4333074/93‑2 (MQ=255)
                                                            cAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGc                             <  2:1805316/93‑1 (MQ=255)
                                                            cAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGc                             >  1:5274126/1‑93 (MQ=255)
                                                             aaTGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCa                            >  2:229006/1‑93 (MQ=255)
                                                              aTGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAg                           <  2:2657008/93‑1 (MQ=255)
                                                              aTGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAg                           >  2:702655/1‑93 (MQ=255)
                                                               tGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGt                          <  2:4550148/93‑1 (MQ=255)
                                                                ggCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTa                         >  1:6682754/1‑93 (MQ=255)
                                                                 gCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTAt                        <  2:4510273/93‑1 (MQ=255)
                                                                 gCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTAt                        <  2:1126278/93‑1 (MQ=255)
                                                                 gCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTAt                        <  2:5253410/93‑1 (MQ=255)
                                                                     ttGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGa                    <  2:6197287/93‑1 (MQ=255)
                                                                        ccGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATgg                 <  1:1217009/93‑1 (MQ=255)
                                                                        ccGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATgg                 >  1:2030701/1‑93 (MQ=255)
                                                                        ccGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATgg                 >  2:5834731/1‑93 (MQ=255)
                                                                         cGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGt                <  1:1363306/93‑1 (MQ=255)
                                                                         cGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGt                <  2:487332/93‑1 (MQ=255)
                                                                         cGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGt                <  2:3865975/93‑1 (MQ=255)
                                                                          gCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGtt               >  2:5296957/1‑93 (MQ=255)
                                                                          gCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGtt               <  2:1206076/93‑1 (MQ=255)
                                                                          gCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGtt               <  1:1660229/93‑1 (MQ=255)
                                                                           cTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTg              >  1:5044274/1‑93 (MQ=255)
                                                                            tgtgAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGa             >  2:1046186/1‑93 (MQ=255)
                                                                            tgtgAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGa             >  1:2842674/1‑93 (MQ=255)
                                                                              tgagtgagTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGAcc           <  2:3446494/93‑1 (MQ=255)
                                                                              tgagtgagTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGAcc           <  2:6007343/93‑1 (MQ=255)
                                                                               gagtgagtCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACcg          >  2:4180677/1‑93 (MQ=255)
                                                                                agtgagtCGGCGCCGTTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACcgc         >  1:4067237/1‑93 (MQ=255)
                                                                                agtgagtCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACcgc         >  1:6160322/1‑93 (MQ=255)
                                                                                  tgagtCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCga       <  2:4943731/93‑1 (MQ=255)
                                                                                     gtCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatga    >  1:3314514/1‑93 (MQ=255)
                                                                                     gtCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatga    <  1:954199/93‑1 (MQ=255)
                                                                                      tCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCgatgat   >  2:2888894/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATG  >  NC_000913/4250998‑4251177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGT  >  NC_000913/4250997‑4251181
                                                                                           |                                                                                             
ATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGC                                                                                             >  SRR4302536.2998577/1‑94 (MQ=60)
  GAAGCGGTATCCCCAGCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCA                                                                                           <  SRR4302536.593868/94‑1 (MQ=60)
  GAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCA                                                                                           <  SRR4302536.993662/94‑1 (MQ=60)
  GAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCA                                                                                           >  SRR4302536.267532/1‑94 (MQ=60)
  GAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCA                                                                                           <  SRR4302536.33973/94‑1 (MQ=60)
   AAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAT                                                                                          >  SRR4302536.4021554/1‑94 (MQ=60)
   AAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAT                                                                                          >  SRR4302536.1084521/1‑94 (MQ=60)
   AAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCAT                                                                                          <  SRR4302536.2471216/94‑1 (MQ=60)
    AGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATT                                                                                         >  SRR4302536.5166187/1‑94 (MQ=60)
    AGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATT                                                                                         <  SRR4302536.2148083/94‑1 (MQ=60)
      CGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGA                                                                                       <  SRR4302536.2490730/94‑1 (MQ=60)
      CGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGA                                                                                       >  SRR4302536.2256155/1‑94 (MQ=60)
      CGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGA                                                                                       >  SRR4302536.518132/1‑94 (MQ=60)
       GGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAG                                                                                      >  SRR4302536.92207/1‑94 (MQ=60)
       GGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAG                                                                                      >  SRR4302536.2569690/1‑94 (MQ=60)
       GGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAG                                                                                      >  SRR4302536.1780431/1‑94 (MQ=60)
        GTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGT                                                                                     >  SRR4302536.3228843/1‑94 (MQ=60)
         TATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTT                                                                                    <  SRR4302536.4725993/94‑1 (MQ=60)
          ATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTG                                                                                   >  SRR4302536.4976411/1‑94 (MQ=60)
          ATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTG                                                                                   >  SRR4302536.5591779/1‑94 (MQ=60)
          ATACCCATCTACCGCAACTGGTGCTGGGCGCGGCGTTTGGCTGGCCCATTCCAATGGCTTTTGCCGCTGTGAGTGCGTCGGCGCCATTGGGTTG                                                                                   >  SRR4302536.6409344/1‑94 (MQ=60)
            ACCCATCTACCGCAAGTGGTTCTGGACGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCT                                                                                 <  SRR4302536.3455925/94‑1 (MQ=60)
            ACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCT                                                                                 >  SRR4302536.4320192/1‑94 (MQ=60)
            ACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCT                                                                                 >  SRR4302536.5524064/1‑94 (MQ=60)
            ACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCT                                                                                 <  SRR4302536.5294424/94‑1 (MQ=60)
             CCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTG                                                                                <  SRR4302536.2332712/94‑1 (MQ=60)
              CCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGG                                                                               >  SRR4302536.1498046/1‑94 (MQ=60)
               CATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGT                                                                              <  SRR4302536.6058464/94‑1 (MQ=60)
               CATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGT                                                                              >  SRR4302536.521899/1‑94 (MQ=60)
                ATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTT                                                                             >  SRR4302536.1734401/1‑94 (MQ=60)
                ATCTACCGCAAGTGGCGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGTGTGAGTCGGCGCCATTGGGCTGCTGGTT                                                                             >  SRR4302536.4216619/1‑94 (MQ=60)
                 TCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCTATTGAGTTGCTGGTTA                                                                            <  SRR4302536.5150947/94‑1 (MQ=60)
                   TACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAAT                                                                          <  SRR4302536.4180070/94‑1 (MQ=60)
                    ACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATG                                                                         <  SRR4302536.4062972/94‑1 (MQ=60)
                     CCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTTCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTTAGTTGCTGGCTAATGT                                                                        >  SRR4302536.5831844/1‑94 (MQ=60)
                     CCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGT                                                                        <  SRR4302536.3251954/94‑1 (MQ=60)
                      CGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTT                                                                       <  SRR4302536.4009694/94‑1 (MQ=60)
                       GCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTC                                                                      >  SRR4302536.4343126/1‑94 (MQ=60)
                        CAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCC                                                                     >  SRR4302536.2431773/1‑94 (MQ=60)
                         AAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCT                                                                    >  SRR4302536.2128120/1‑94 (MQ=60)
                         AAGTGGTGCTGGGCGCGGCGCTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCT                                                                    >  SRR4302536.6740338/1‑94 (MQ=60)
                          AGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTC                                                                   >  SRR4302536.4182152/1‑94 (MQ=60)
                            TGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGC                                                                 >  SRR4302536.1986010/1‑94 (MQ=60)
                              GTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCA                                                               <  SRR4302536.1419734/94‑1 (MQ=60)
                               TGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAA                                                              >  SRR4302536.1046335/1‑94 (MQ=60)
                                GCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAAT                                                             <  SRR4302536.4949012/94‑1 (MQ=60)
                                 CTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATA                                                            >  SRR4302536.5540526/1‑94 (MQ=60)
                                 CTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATA                                                            <  SRR4302536.666429/94‑1 (MQ=60)
                                 CTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATA                                                            <  SRR4302536.4062052/94‑1 (MQ=60)
                                  TGGTCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATAT                                                           <  SRR4302536.2440026/94‑1 (MQ=60)
                                  TGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATAT                                                           <  SRR4302536.470985/94‑1 (MQ=60)
                                  TGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATAT                                                           <  SRR4302536.4359894/94‑1 (MQ=60)
                                   GGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATT                                                          <  SRR4302536.5705100/94‑1 (MQ=60)
                                   GGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATT                                                          <  SRR4302536.3075194/94‑1 (MQ=60)
                                    GGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTC                                                         <  SRR4302536.5993228/94‑1 (MQ=60)
                                     GCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCT                                                        <  SRR4302536.6292419/94‑1 (MQ=60)
                                     GCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCT                                                        >  SRR4302536.3591219/1‑94 (MQ=60)
                                       GCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCT                                                      <  SRR4302536.3731166/94‑1 (MQ=60)
                                       GCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCT                                                      >  SRR4302536.4709459/1‑94 (MQ=60)
                                         GGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGG                                                    >  SRR4302536.1395153/1‑94 (MQ=60)
                                         GGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGG                                                    >  SRR4302536.6365279/1‑94 (MQ=60)
                                         GGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGG                                                    >  SRR4302536.3273641/1‑94 (MQ=60)
                                         GGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGG                                                    <  SRR4302536.3003790/94‑1 (MQ=60)
                                            GTTTGGCTGGTCGATTCCAATGGCTTTTGTTGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCCATCTTCTCCGGGCG                                                 >  SRR4302536.3862717/1‑94 (MQ=60)
                                              TTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGT                                               <  SRR4302536.2654118/94‑1 (MQ=60)
                                              TGGGCTGGTCGATTCCAATGGCTTGTGCGTCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGT                                               <  SRR4302536.1968268/94‑1 (MQ=60)
                                                   TGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTT                                          >  SRR4302536.4327895/1‑94 (MQ=60)
                                                     GTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTAC                                        <  SRR4302536.3753759/94‑1 (MQ=60)
                                                        GATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGAC                                     >  SRR4302536.4001011/1‑94 (MQ=60)
                                                         ATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACA                                    >  SRR4302536.2493716/1‑94 (MQ=60)
                                                         ATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACA                                    <  SRR4302536.3884560/94‑1 (MQ=60)
                                                          TTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACAC                                   >  SRR4302536.5005737/1‑94 (MQ=60)
                                                            CCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGC                                 >  SRR4302536.5431317/1‑94 (MQ=60)
                                                            CCAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTACTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGC                                 >  SRR4302536.5118204/1‑94 (MQ=60)
                                                             CAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGGA                                <  SRR4302536.4462844/94‑1 (MQ=60)
                                                             CAATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCA                                >  SRR4302536.1647398/1‑94 (MQ=60)
                                                              AATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAG                               >  SRR4302536.5100144/1‑94 (MQ=60)
                                                               ATGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGT                              >  SRR4302536.1001381/1‑94 (MQ=60)
                                                                TGGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTA                             >  SRR4302536.6881252/1‑94 (MQ=60)
                                                                 GGCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTAT                            <  SRR4302536.4372892/94‑1 (MQ=60)
                                                                  GCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATG                           <  SRR4302536.6725539/94‑1 (MQ=60)
                                                                  GCTTTTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATG                           >  SRR4302536.4541891/1‑94 (MQ=60)
                                                                      TTGCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGAT                       <  SRR4302536.734658/94‑1 (MQ=60)
                                                                        GCCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGG                     >  SRR4302536.2091840/1‑94 (MQ=60)
                                                                         CCGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGT                    <  SRR4302536.1253570/94‑1 (MQ=60)
                                                                          CGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTT                   <  SRR4302536.1717696/94‑1 (MQ=60)
                                                                          CGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTT                   <  SRR4302536.3698945/94‑1 (MQ=60)
                                                                          CGCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTT                   <  SRR4302536.1403936/94‑1 (MQ=60)
                                                                           GCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTG                  >  SRR4302536.5195446/1‑94 (MQ=60)
                                                                           GCTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTG                  <  SRR4302536.1708424/94‑1 (MQ=60)
                                                                            CTGTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGA                 >  SRR4302536.2926768/1‑94 (MQ=60)
                                                                              GTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACC               >  SRR4302536.991015/1‑94 (MQ=60)
                                                                              GTGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACC               >  SRR4302536.6324954/1‑94 (MQ=60)
                                                                              GTGAGGGAGTCGGCGCCATTGCGATGCTGGTTAATGTTGCTCTCCAATATTCTCTGGGCGGTGGCTTACGACACGCACTATGCGATGGTTCACC               >  SRR4302536.4840525/1‑94 (MQ=60)
                                                                               TGAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCG              >  SRR4302536.5263291/1‑94 (MQ=60)
                                                                                GAGTGAGTCGGCGCCGTTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGC             >  SRR4302536.4187789/1‑94 (MQ=60)
                                                                                GAGTGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGC             >  SRR4302536.6343830/1‑94 (MQ=60)
                                                                                   TGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGAT          <  SRR4302536.2244056/94‑1 (MQ=60)
                                                                                   TGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTGGACCGCGAT          <  SRR4302536.6744665/94‑1 (MQ=60)
                                                                                   TGAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGCTGACCGCGAT          >  SRR4302536.4727438/1‑94 (MQ=60)
                                                                                    GAGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATG         <  SRR4302536.5464764/94‑1 (MQ=60)
                                                                                     AGTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGA        >  SRR4302536.3412476/1‑94 (MQ=60)
                                                                                      GTCGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGAT       <  SRR4302536.982404/94‑1 (MQ=60)
                                                                                        CGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCCATATTCTCTGGGCGGTGGCTTACGACACGCAGCATGCGATGGTTGACCGCGATGATGA     >  SRR4302536.5861250/1‑94 (MQ=60)
                                                                                        CGGCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGA     <  SRR4302536.3045811/94‑1 (MQ=60)
                                                                                        CGGCGCCATTGAGTTCTTGTTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGA     <  SRR4302536.145715/94‑1 (MQ=60)
                                                                                          GCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATG   >  SRR4302536.832481/1‑94 (MQ=60)
                                                                                          GCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATG   <  SRR4302536.1382986/94‑1 (MQ=60)
                                                                                          GCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATG   >  SRR4302536.5224343/1‑94 (MQ=60)
                                                                                          GCGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATG   >  SRR4302536.5944302/1‑94 (MQ=60)
                                                                                           CGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGT  >  SRR4302536.110583/1‑94 (MQ=60)
                                                                                           CGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGT  >  SRR4302536.614725/1‑94 (MQ=60)
                                                                                           CGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGT  >  SRR4302536.3406318/1‑94 (MQ=60)
                                                                                           |                                                                                             
ATGAAGCGGTATACCCATCTACCGCAAGTGGTGCTGGGCGCGGCGTTTGGCTGGTCGATTCCAATGGCTTTTGCCGCTGTGAGTGAGTCGGTGCCATTGAGTTGCTGGTTAATGTTCCTCGCCAATATTCTCTGGGCGGTGGCTTACGACACGCAGTATGCGATGGTTGACCGCGATGATGATGT  >  NC_000913/4250997‑4251181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑