Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,235 A→G C406R (TGC→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2350AG98.2% 518.8 / ‑1.8 167C406R (TGC→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (83/81);  total (85/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 176 total aligned reads displayed.

TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324
                                                                                          |                                                                                         
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  2:4050714/1‑93 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCCGCTGGCGCGCgggc                                                                                      >  2:1707438/1‑91 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  2:3580094/1‑93 (MQ=255)
     aTGTCGTAGATAGGAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGCCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    <  2:3611048/93‑1 (MQ=255)
     aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    <  1:4066265/93‑1 (MQ=255)
     aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    <  2:390882/93‑1 (MQ=255)
     aTGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGa                                                                                    >  1:1191384/1‑93 (MQ=255)
      tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                                                   >  2:4550182/1‑93 (MQ=255)
        tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                                                 <  2:3010315/93‑1 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            >  2:3702849/1‑93 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            <  1:2561377/93‑1 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  1:1243085/1‑93 (MQ=255)
                aGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGCCGATTACTGATaaa                                                                         >  2:1612869/1‑93 (MQ=255)
                 gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                                        >  1:321731/1‑93 (MQ=255)
                  caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                                                       >  1:1401840/1‑93 (MQ=255)
                   aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                                                      >  1:626208/1‑93 (MQ=255)
                    ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                                                     >  1:1022293/1‑93 (MQ=255)
                    ggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTg                                                                     <  1:1550718/93‑1 (MQ=255)
                     gcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTgg                                                                    >  2:3084914/1‑93 (MQ=255)
                      cagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGc                                                                   <  2:473558/93‑1 (MQ=255)
                       agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                                                  >  1:2453305/1‑93 (MQ=255)
                       agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTAGCt                                                                  <  2:4559390/93‑1 (MQ=255)
                         cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                                                <  2:2423256/93‑1 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  1:4574253/1‑93 (MQ=255)
                            cgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                                             <  1:2403979/93‑1 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            <  2:1892287/93‑1 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            <  2:2783434/93‑1 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  1:2681842/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  2:1284896/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           <  2:1349117/93‑1 (MQ=255)
                              tATTCTCGACATACACGGGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAattatt                                                           >  2:1165867/1‑93 (MQ=255)
                              tATTCTCGACATACACGCGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           <  1:1189572/93‑1 (MQ=255)
                               tttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  1:1027230/92‑1 (MQ=255)
                               aTTCTCGACATACACGTGGGGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:1611393/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:2868142/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          >  1:2055283/1‑93 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  2:559161/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCACGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  1:4478819/93‑1 (MQ=255)
                                ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                                         >  2:2040453/1‑93 (MQ=255)
                                 tctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgc                                                        >  1:3774445/1‑93 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       <  1:513906/93‑1 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       >  2:1214995/1‑93 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       >  2:3098006/1‑93 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       >  1:1758132/1‑93 (MQ=255)
                                  ctcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcgcg                                                       <  1:4020930/93‑1 (MQ=255)
                                    cGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTa                                                     >  2:741874/1‑93 (MQ=255)
                                     gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                                                    >  1:243071/1‑93 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   >  1:4141083/1‑93 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   <  1:3136055/93‑1 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   >  2:4478124/1‑93 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGTGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 <  2:4643022/93‑1 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 <  1:4695696/93‑1 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 >  2:3803111/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  2:2836247/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  1:2473459/1‑93 (MQ=255)
                                           cacGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGCACCGCGCt                                              <  1:4325682/93‑1 (MQ=255)
                                                ggCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAtt                                         <  1:1490063/93‑1 (MQ=255)
                                                   gcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCg                                      <  1:3799802/93‑1 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   >  2:134195/1‑93 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   >  1:3005772/1‑93 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   >  1:3897183/1‑93 (MQ=255)
                                                      cTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTAGCGTACCGCGCTGGATTCCGtct                                   >  1:735679/1‑93 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  <  1:18962/93‑1 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  <  1:3453720/93‑1 (MQ=255)
                                                        tCCGGACGCAGGCGCGTCGCTTCGCAGCTGGCGCGCGGACTATTACTGATAACCTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  2:3246351/93‑1 (MQ=255)
                                                         cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt                                <  1:4158748/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  2:2827242/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  2:3224952/93‑1 (MQ=255)
                                                          ccGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               >  1:2768571/1‑93 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              >  2:2502763/1‑93 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              >  1:831399/1‑93 (MQ=255)
                                                            gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat                             <  2:1874222/93‑1 (MQ=255)
                                                            gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat                             <  2:3508829/93‑1 (MQ=255)
                                                               gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgc                          >  2:3291499/1‑93 (MQ=255)
                                                                 aggcgcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcgg                        >  1:3179038/4‑93 (MQ=255)
                                                                  ggcgcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAgcggc                       <  2:3832009/91‑1 (MQ=255)
                                                                      cgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTc                   >  2:1533913/1‑93 (MQ=255)
                                                                      cgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTc                   <  2:2561351/93‑1 (MQ=255)
                                                                       gTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTcc                  >  1:2347182/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  2:1161069/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  1:2833788/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAATTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 >  1:621781/1‑93 (MQ=255)
                                                                        gcccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 <  2:2249720/92‑1 (MQ=255)
                                                                         cccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCat                >  1:1045612/1‑93 (MQ=255)
                                                                         cccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCat                >  1:1831033/1‑93 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  2:4355574/93‑1 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               >  1:3990565/1‑93 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              >  2:800368/1‑93 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              >  1:2638330/1‑93 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              <  2:2210644/93‑1 (MQ=255)
                                                                            ttCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCCGCGCTCCAtatt             >  1:1902769/1‑93 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            <  2:1507769/93‑1 (MQ=255)
                                                                               gCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATg          <  1:2185839/93‑1 (MQ=255)
                                                                                cAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgc         <  2:178736/93‑1 (MQ=255)
                                                                                 aGCTGGCGCGCGGACGATTACTGATAAGCTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgca        <  1:1092279/93‑1 (MQ=255)
                                                                                 aGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATtca        >  2:165690/1‑93 (MQ=255)
                                                                                 aGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgca        <  2:1690240/93‑1 (MQ=255)
                                                                                   cTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagc      <  2:490800/93‑1 (MQ=255)
                                                                                      gcgcgcGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACg   <  1:3358045/93‑1 (MQ=255)
                                                                                      gcgcgcGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACg   >  1:249917/1‑93 (MQ=255)
                                                                                          |                                                                                         
TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270143‑4270328
                                                                                            |                                                                                             
AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGC                                                                                              >  SRR4302544.3597938/1‑94 (MQ=60)
 ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             >  SRR4302544.2018610/1‑94 (MQ=60)
 ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302544.4736150/94‑1 (MQ=60)
  TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            >  SRR4302544.4634965/1‑94 (MQ=60)
      CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGCCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACat                                                                                        >  SRR4302544.2313273/1‑92 (MQ=60)
      CATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGA                                                                                        >  SRR4302544.1203204/1‑94 (MQ=60)
       ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302544.4106752/94‑1 (MQ=60)
          GCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                                                    <  SRR4302544.1188844/94‑1 (MQ=60)
              AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                >  SRR4302544.109074/1‑94 (MQ=60)
               GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               <  SRR4302544.2585981/94‑1 (MQ=60)
               GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               >  SRR4302544.1255352/1‑94 (MQ=60)
                ATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAA                                                                              <  SRR4302544.2136940/94‑1 (MQ=60)
                  AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            >  SRR4302544.325071/1‑94 (MQ=60)
                  AGCAAGCAGCGGCGTATTCTCCACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            <  SRR4302544.1050036/94‑1 (MQ=60)
                   GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           >  SRR4302544.1415805/1‑94 (MQ=60)
                    CAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTT                                                                          >  SRR4302544.632366/1‑94 (MQ=60)
                     AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                                         >  SRR4302544.1032502/1‑94 (MQ=60)
                     AGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTG                                                                         <  SRR4302544.4008649/94‑1 (MQ=60)
                      GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                                                        <  SRR4302544.632303/94‑1 (MQ=60)
                      GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                                                        <  SRR4302544.1566166/94‑1 (MQ=60)
                        CAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCT                                                                      >  SRR4302544.2476709/1‑94 (MQ=60)
                            GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302544.4567746/1‑94 (MQ=60)
                            GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302544.4619836/1‑94 (MQ=60)
                            GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302544.2437786/1‑94 (MQ=60)
                             GCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTC                                                                 <  SRR4302544.2472358/94‑1 (MQ=60)
                              CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302544.727691/1‑94 (MQ=60)
                              CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302544.2426796/94‑1 (MQ=60)
                               GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302544.2707541/1‑94 (MQ=60)
                               GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               >  SRR4302544.1658997/1‑94 (MQ=60)
                                TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302544.2075060/1‑94 (MQ=60)
                                TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302544.3173060/1‑94 (MQ=60)
                                TATTCTCGACATACACGCGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              <  SRR4302544.1201380/94‑1 (MQ=60)
                                 TTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302544.1037475/94‑1 (MQ=60)
                                 ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302544.3884736/94‑1 (MQ=60)
                                 ATTCTCGACATACACGTGGCACGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302544.4523844/94‑1 (MQ=60)
                                  TTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGC                                                            >  SRR4302544.3811852/1‑94 (MQ=60)
                                   TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                                           >  SRR4302544.1775337/1‑94 (MQ=60)
                                   TCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCG                                                           >  SRR4302544.1775338/1‑94 (MQ=60)
                                    CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          <  SRR4302544.4060952/94‑1 (MQ=60)
                                    CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          <  SRR4302544.518982/94‑1 (MQ=60)
                                      CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        >  SRR4302544.245598/1‑94 (MQ=60)
                                      CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        >  SRR4302544.827094/1‑94 (MQ=60)
                                       GACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACC                                                       >  SRR4302544.4182079/1‑94 (MQ=60)
                                        ACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                                                      <  SRR4302544.3165844/94‑1 (MQ=60)
                                          ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    <  SRR4302544.3783655/94‑1 (MQ=60)
                                          ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    >  SRR4302544.2497108/1‑94 (MQ=60)
                                          ATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCG                                                    <  SRR4302544.4742062/94‑1 (MQ=60)
                                           TACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                                                   >  SRR4302544.186341/1‑94 (MQ=60)
                                             CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGCACCGCGCTG                                                 <  SRR4302544.4369731/94‑1 (MQ=60)
                                                  GGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTC                                            <  SRR4302544.1504794/94‑1 (MQ=60)
                                                     GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                                         <  SRR4302544.3837438/94‑1 (MQ=60)
                                                      CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        <  SRR4302544.3105809/94‑1 (MQ=60)
                                                       GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       >  SRR4302544.3034587/1‑94 (MQ=60)
                                                       GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       >  SRR4302544.3034586/1‑94 (MQ=60)
                                                       GCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCT                                       >  SRR4302544.3935889/1‑94 (MQ=60)
                                                       GCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTAGCGTACCGCGCTGGATTCCGTCT                                       >  SRR4302544.742876/1‑94 (MQ=60)
                                                         TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     <  SRR4302544.3486693/94‑1 (MQ=60)
                                                         TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     <  SRR4302544.19152/94‑1 (MQ=60)
                                                           CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302544.4199942/94‑1 (MQ=60)
                                                           CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302544.4366825/94‑1 (MQ=60)
                                                           CCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   >  SRR4302544.2795205/1‑94 (MQ=60)
                                                            CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  >  SRR4302544.839575/1‑94 (MQ=60)
                                                             CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 >  SRR4302544.346444/1‑94 (MQ=60)
                                                              GACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA                                >  SRR4302544.2615013/1‑94 (MQ=60)
                                                               gcgcaggcaCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302544.418415/85‑1 (MQ=60)
                                                               ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302544.1316063/94‑1 (MQ=60)
                                                                 GCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCG                             >  SRR4302544.3305653/1‑94 (MQ=60)
                                                                  CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            >  SRR4302544.3209291/1‑94 (MQ=60)
                                                                   AGGCGCGTCCCTTCGCAGCTCGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC                           <  SRR4302544.1455765/94‑1 (MQ=60)
                                                                       GCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTC                       >  SRR4302544.2480824/1‑94 (MQ=60)
                                                                        CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302544.2369417/1‑94 (MQ=60)
                                                                         GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     <  SRR4302544.475902/94‑1 (MQ=60)
                                                                         GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302544.2861169/1‑94 (MQ=60)
                                                                         GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302544.4117472/1‑94 (MQ=60)
                                                                         GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAATTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302544.627880/1‑94 (MQ=60)
                                                                          TCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAT                    >  SRR4302544.1055984/1‑94 (MQ=60)
                                                                          TCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAT                    >  SRR4302544.1848836/1‑94 (MQ=60)
                                                                           CCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATA                   >  SRR4302544.4030286/1‑94 (MQ=60)
                                                                            CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  >  SRR4302544.2663618/1‑94 (MQ=60)
                                                                             CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCCGCGCTCCATATT                 >  SRR4302544.1921155/1‑94 (MQ=60)
                                                                              TTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTA                <  SRR4302544.2791189/94‑1 (MQ=60)
                                                                                 GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             <  SRR4302544.2206509/94‑1 (MQ=60)
                                                                                   AGCTGGCGCGCGGACGATTACTGATAAGCTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           <  SRR4302544.1103149/94‑1 (MQ=60)
                                                                                   AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           >  SRR4302544.2697106/1‑94 (MQ=60)
                                                                                       GGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACG       <  SRR4302544.1220725/94‑1 (MQ=60)
                                                                                       GGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACG       >  SRR4302544.252540/1‑94 (MQ=60)
                                                                                       GGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACG       <  SRR4302544.4047620/94‑1 (MQ=60)
                                                                                        GCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC      <  SRR4302544.3390090/94‑1 (MQ=60)
                                                                                         CGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCC     <  SRR4302544.2558515/94‑1 (MQ=60)
                                                                                          GCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCT    <  SRR4302544.4530606/94‑1 (MQ=60)
                                                                                          GCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCT    >  SRR4302544.4140572/1‑94 (MQ=60)
                                                                                          GCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCT    <  SRR4302544.1215540/94‑1 (MQ=60)
                                                                                            GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  <  SRR4302544.1525199/94‑1 (MQ=60)
                                                                                            GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  SRR4302544.4461150/1‑94 (MQ=60)
                                                                                            |                                                                                             
AATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270143‑4270328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑