Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,235 A→G C406R (TGC→CGC)  uvrA ← excision nuclease subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,2350AG100.0% 886.1 / NA 274C406R (TGC→CGC) uvrAexcision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (132/142);  total (132/142)

Only 100 of 284 total aligned reads displayed.

TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324
                                                                                          |                                                                                         
tGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGcgcgcg                                                                                         >  2:3050328/1‑93 (MQ=255)
 gCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCgg                                                                                        <  2:76646/93‑1 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  2:6000078/1‑93 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  1:872674/1‑93 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  2:3101747/1‑93 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  2:325138/1‑93 (MQ=255)
   tCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAc                                                                                      >  1:1730823/1‑93 (MQ=255)
      tGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAt                                                                                   <  1:4610703/93‑1 (MQ=255)
       gTCGTAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAtt                                                                                  <  2:4472723/93‑1 (MQ=255)
        tCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTa                                                                                 >  2:1785363/1‑93 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               >  1:5791589/1‑93 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               <  1:2474468/93‑1 (MQ=255)
          ggAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACt                                                                               <  1:422255/93‑1 (MQ=255)
           gagaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTg                                                                              <  2:2116148/93‑1 (MQ=255)
            agaTAGTAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  1:6449760/93‑1 (MQ=255)
            agaTAGCAGGCAGCTGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  1:2182240/93‑1 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  2:4796410/93‑1 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  1:2813655/93‑1 (MQ=255)
            agaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGa                                                                             <  1:5352465/93‑1 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            <  2:5934562/93‑1 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            >  1:3382798/1‑93 (MQ=255)
             gaTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAt                                                                            <  1:3971335/93‑1 (MQ=255)
              aTAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATa                                                                           >  2:5859160/1‑93 (MQ=255)
               tAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCATTCGCAGCTGGCGCGCGGACGATTACTGATaa                                                                          >  2:420602/1‑93 (MQ=255)
                aGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATaaa                                                                         >  1:5561663/1‑93 (MQ=255)
                 gcaggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAc                                                                        <  1:5905516/93‑1 (MQ=255)
                  caggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                                                       >  1:19266/1‑93 (MQ=255)
                  caggcagCAGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACt                                                                       >  1:6564872/1‑93 (MQ=255)
                   aggcagCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACtt                                                                      >  2:2613388/1‑93 (MQ=255)
                       agCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCt                                                                  <  1:4445257/93‑1 (MQ=255)
                         cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                                                >  2:1217777/1‑93 (MQ=255)
                         cggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTaa                                                                >  2:4374691/1‑93 (MQ=255)
                          ggcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAt                                                               >  1:3919070/1‑93 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              >  1:158739/1‑93 (MQ=255)
                           gcgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAtt                                                              <  2:3163287/93‑1 (MQ=255)
                            tggaTTCTCGCCATACACGTGGCGCGCTTCCCGACGAGGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                                             <  1:727478/90‑1 (MQ=255)
                            cgTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttc                                                             <  2:3601222/93‑1 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            >  1:1041720/1‑93 (MQ=255)
                             gTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttct                                                            >  1:5263702/1‑93 (MQ=255)
                              tATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttctt                                                           >  2:4965610/1‑93 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          <  1:1652381/93‑1 (MQ=255)
                               aTTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAttcttc                                                          >  2:3868197/1‑93 (MQ=255)
                                ttCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTcg                                                         <  1:3208633/93‑1 (MQ=255)
                                   tcGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGt                                                      >  1:3550980/1‑93 (MQ=255)
                                     gACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAc                                                    >  1:2058100/1‑93 (MQ=255)
                                      aCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAcc                                                   >  2:5406506/1‑93 (MQ=255)
                                       cATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcg                                                  <  2:4317837/93‑1 (MQ=255)
                                        aTACACGTGGCGCGCTTTCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGGTAATTCTTAGTGTACtgc                                                 >  2:2313942/1‑93 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 >  1:2999514/1‑93 (MQ=255)
                                        aTACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgc                                                 >  1:2092768/1‑93 (MQ=255)
                                         tACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACcgcg                                                >  2:3974216/1‑93 (MQ=255)
                                            acGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTg                                             <  2:5024061/93‑1 (MQ=255)
                                               tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                                          <  1:4938761/93‑1 (MQ=255)
                                               tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                                          <  1:5217737/93‑1 (MQ=255)
                                               tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                                          <  2:2262961/93‑1 (MQ=255)
                                               tGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAt                                          <  2:2699062/93‑1 (MQ=255)
                                                ggCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAtt                                         <  2:6233061/93‑1 (MQ=255)
                                                 gcgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGTTTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTc                                        <  2:5149557/93‑1 (MQ=255)
                                                  cgcgcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGATGGATTcc                                       >  2:2359604/1‑93 (MQ=255)
                                                     gcTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtc                                    <  2:461655/93‑1 (MQ=255)
                                                      cTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtct                                   <  1:1617789/93‑1 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  >  2:1593262/1‑93 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  <  2:4939218/93‑1 (MQ=255)
                                                       ttCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGtctc                                  <  2:4104353/93‑1 (MQ=255)
                                                        tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  2:3491495/93‑1 (MQ=255)
                                                        tCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTctct                                 <  1:1331214/93‑1 (MQ=255)
                                                         cccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCtt                                <  2:3343594/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               <  1:2629345/93‑1 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               >  2:3624525/1‑93 (MQ=255)
                                                          ccGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCttt                               >  2:6093046/1‑93 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              <  2:5483224/93‑1 (MQ=255)
                                                           cGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTta                              >  1:5456824/1‑93 (MQ=255)
                                                            gACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtat                             >  1:1597432/1‑93 (MQ=255)
                                                             aCGCAGGCGCGTCCCTTCGCGGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata                            >  1:6082866/1‑93 (MQ=255)
                                                             aCGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTtata                            <  1:1212668/93‑1 (MQ=255)
                                                              cGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAg                           >  2:6681215/1‑93 (MQ=255)
                                                               gCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTTGCGTACCGCGCTGGATTCCGTCTCTTTATAgc                          >  2:5762492/1‑93 (MQ=255)
                                                                    cgcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGgcgc                     <  1:6327410/93‑1 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    <  2:1856556/93‑1 (MQ=255)
                                                                     gcgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCt                    >  1:6200439/1‑93 (MQ=255)
                                                                      cgTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTc                   >  2:3728146/1‑93 (MQ=255)
                                                                       gTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTcc                  >  1:6252822/1‑93 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 <  2:896466/93‑1 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 <  2:635168/93‑1 (MQ=255)
                                                                        tCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCa                 <  1:5624170/93‑1 (MQ=255)
                                                                         cccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCat                >  2:4135365/1‑93 (MQ=255)
                                                                          ccTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCata               <  1:1437624/93‑1 (MQ=255)
                                                                           cTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCatat              <  2:5601702/93‑1 (MQ=255)
                                                                             tCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAtatta            <  1:4867697/93‑1 (MQ=255)
                                                                                cAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgc         >  2:2032107/1‑93 (MQ=255)
                                                                                  gCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcag       <  2:1004111/93‑1 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     >  1:1024095/1‑93 (MQ=255)
                                                                                    tGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATgcagca     <  2:3743977/93‑1 (MQ=255)
                                                                                     ggCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGTAGCAc    <  1:3747227/93‑1 (MQ=255)
                                                                                     ggCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAc    <  2:2811397/93‑1 (MQ=255)
                                                                                     ggCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAc    >  2:6171155/1‑93 (MQ=255)
                                                                                     ggCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAc    <  2:4832509/93‑1 (MQ=255)
                                                                                     ggCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAc    <  1:183340/93‑1 (MQ=255)
                                                                                       cgcgcgGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGc  <  2:2349818/93‑1 (MQ=255)
                                                                                       cgcgcgGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGc  <  2:6505620/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC  >  NC_000913/4270145‑4270324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328
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CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCG                                                                                               >  SRR4302536.2990594/1‑94 (MQ=60)
  ATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCG                                                                                             <  SRR4302536.6319018/94‑1 (MQ=60)
   TGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGG                                                                                            <  SRR4302536.5759695/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          <  SRR4302536.5318390/94‑1 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          >  SRR4302536.1780780/1‑94 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          >  SRR4302536.898381/1‑94 (MQ=60)
     CTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGAC                                                                                          >  SRR4302536.6080434/1‑94 (MQ=60)
      TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                                         <  SRR4302536.6684090/94‑1 (MQ=60)
      TCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACG                                                                                         <  SRR4302536.5611176/94‑1 (MQ=60)
        ATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       >  SRR4302536.6658826/1‑94 (MQ=60)
        ACGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCCCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGAT                                                                                       <  SRR4302536.4803392/94‑1 (MQ=60)
         TGTGGGAGAGAGGAGGGAGCGGCGTATTCTCGACATACGCGGGGCGCGCTTCCCGCAGCCGGCGCGTCCCTTCGCAGCTGGGGCGCGGACGATT                                                                                      <  SRR4302536.3114342/94‑1 (MQ=28)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACCCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      <  SRR4302536.6349305/94‑1 (MQ=60)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      <  SRR4302536.4749200/94‑1 (MQ=60)
         TGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATT                                                                                      <  SRR4302536.1763910/94‑1 (MQ=60)
          GTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTA                                                                                     >  SRR4302536.3866424/1‑94 (MQ=60)
           TCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAC                                                                                    <  SRR4302536.3910889/94‑1 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                                                   >  SRR4302536.5964001/1‑94 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                                                   <  SRR4302536.1992587/94‑1 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACT                                                                                   >  SRR4302536.1124468/1‑94 (MQ=60)
            CGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTAAT                                                                                   <  SRR4302536.3584839/94‑1 (MQ=60)
             GGTGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302536.1856251/94‑1 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302536.2549165/94‑1 (MQ=60)
             GGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTG                                                                                  <  SRR4302536.433391/94‑1 (MQ=60)
              GAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGA                                                                                 <  SRR4302536.1330816/94‑1 (MQ=60)
               AGATAGTAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302536.6642407/94‑1 (MQ=60)
               AGATAGCGGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302536.261000/94‑1 (MQ=60)
               AGATAGCAGGCAGCTGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302536.2248018/94‑1 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                >  SRR4302536.5750471/1‑94 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                >  SRR4302536.3483346/1‑94 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302536.2897033/94‑1 (MQ=60)
               AGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGAT                                                                                <  SRR4302536.5512225/94‑1 (MQ=60)
                GATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATA                                                                               <  SRR4302536.4089213/94‑1 (MQ=60)
                  TAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAA                                                                             >  SRR4302536.5727289/1‑94 (MQ=60)
                   AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            >  SRR4302536.3686130/1‑94 (MQ=60)
                   AGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAAC                                                                            <  SRR4302536.1662419/94‑1 (MQ=60)
                    GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           >  SRR4302536.19806/1‑94 (MQ=60)
                    GCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           <  SRR4302536.6081386/94‑1 (MQ=60)
                    GCAGGCAGCAGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACT                                                                           >  SRR4302536.6760272/1‑94 (MQ=60)
                       GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                                                        <  SRR4302536.5425410/94‑1 (MQ=60)
                       GGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGG                                                                        <  SRR4302536.4274979/94‑1 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     <  SRR4302536.4578746/94‑1 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     >  SRR4302536.2944930/1‑94 (MQ=60)
                          AGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTA                                                                     <  SRR4302536.2219448/94‑1 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                                                   <  SRR4302536.5975591/94‑1 (MQ=60)
                            CGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAAT                                                                   >  SRR4302536.4035559/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  >  SRR4302536.162845/1‑94 (MQ=60)
                             GGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATT                                                                  <  SRR4302536.5030003/94‑1 (MQ=60)
                               tggATTCTCGCCATACACGTGGCGCGCTTCCCGACGAGGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302536.748772/91‑1 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302536.5420584/1‑94 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302536.1072685/1‑94 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                >  SRR4302536.1700344/1‑94 (MQ=60)
                               CGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCT                                                                <  SRR4302536.4081249/94‑1 (MQ=60)
                                GTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTT                                                               <  SRR4302536.872904/94‑1 (MQ=60)
                                 TATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTC                                                              >  SRR4302536.778461/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302536.4727765/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302536.96403/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302536.1700402/94‑1 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             >  SRR4302536.6465022/1‑94 (MQ=60)
                                  ATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCG                                                             <  SRR4302536.585426/94‑1 (MQ=60)
                                   TTCTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGC                                                            <  SRR4302536.3302767/94‑1 (MQ=60)
                                     CTCGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGT                                                          >  SRR4302536.3657149/1‑94 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTGGCAGCTGGAGCGCGGACGTTTACTGATAAACTTGGCTAATcgctcgcggac                                                        >  SRR4302536.940795/1‑83 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        >  SRR4302536.4737035/1‑94 (MQ=60)
                                       CGACATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTAC                                                        >  SRR4302536.2120090/1‑94 (MQ=60)
                                         GCATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCG                                                      <  SRR4302536.2154804/94‑1 (MQ=60)
                                          CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                                                     >  SRR4302536.3087699/1‑94 (MQ=60)
                                          CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                                                     >  SRR4302536.2155731/1‑94 (MQ=60)
                                          CATACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGC                                                     >  SRR4302536.2785630/1‑94 (MQ=60)
                                            taacCGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGC                                                   <  SRR4302536.587839/90‑1 (MQ=60)
                                             ACACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCT                                                  >  SRR4302536.1961153/1‑94 (MQ=60)
                                              CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                                                 <  SRR4302536.1971285/94‑1 (MQ=60)
                                              CACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTG                                                 <  SRR4302536.1637475/94‑1 (MQ=60)
                                               ACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGG                                                <  SRR4302536.2081966/94‑1 (MQ=60)
                                               ACGTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGG                                                >  SRR4302536.1637630/1‑94 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTTTTCGCGTACCGCGCTGGAT                                              >  SRR4302536.3554284/1‑94 (MQ=60)
                                                 GTGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGAT                                              <  SRR4302536.1850708/94‑1 (MQ=60)
                                                  TGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATT                                             <  SRR4302536.5087176/94‑1 (MQ=60)
                                                  TGGCGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATT                                             <  SRR4302536.5373211/94‑1 (MQ=60)
                                                     CGCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCG                                          >  SRR4302536.1088668/1‑94 (MQ=60)
                                                      GCGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGT                                         >  SRR4302536.2979582/1‑94 (MQ=60)
                                                       CGCTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTC                                        <  SRR4302536.5415238/94‑1 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      <  SRR4302536.1664927/94‑1 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      <  SRR4302536.5080464/94‑1 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      >  SRR4302536.5648951/1‑94 (MQ=60)
                                                         CTTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTC                                      <  SRR4302536.6782839/94‑1 (MQ=60)
                                                          TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     <  SRR4302536.5518633/94‑1 (MQ=60)
                                                          TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     >  SRR4302536.966407/1‑94 (MQ=60)
                                                          TTCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCT                                     <  SRR4302536.2719062/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302536.1260098/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302536.1370954/94‑1 (MQ=60)
                                                           TCCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTT                                    <  SRR4302536.1691022/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302536.4120589/94‑1 (MQ=60)
                                                            CCCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTT                                   <  SRR4302536.6581517/94‑1 (MQ=60)
                                                             CCGAGGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAACCTTGGCTAATTCTTCGCGTACCGCGCTGGATT‑CGTCTCTTTAT                                 <  SRR4302536.3149665/94‑1 (MQ=60)
                                                             CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  <  SRR4302536.2708178/94‑1 (MQ=60)
                                                             CCGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTA                                  >  SRR4302536.5619991/1‑94 (MQ=60)
                                                              CGACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTAT                                 >  SRR4302536.1644092/1‑94 (MQ=60)
                                                               GACGCAGGCGCGTCCCTTCGCGGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATA                                >  SRR4302536.6264308/1‑94 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTCCCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302536.1596468/94‑1 (MQ=60)
                                                                ACGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAG                               <  SRR4302536.1249097/94‑1 (MQ=60)
                                                                 CGCAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGC                              >  SRR4302536.4057890/1‑94 (MQ=60)
                                                                   CAGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGG                            <  SRR4302536.6312164/94‑1 (MQ=60)
                                                                    AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTACTTCTTCGCGTACCGCGCTTGATTCCGTCTCTTTATAGCGGC                           >  SRR4302536.2242190/1‑94 (MQ=60)
                                                                    AGGCGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGC                           <  SRR4302536.435884/94‑1 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        >  SRR4302536.6385115/1‑94 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        <  SRR4302536.6515859/94‑1 (MQ=60)
                                                                       CGCGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCT                        <  SRR4302536.1734044/94‑1 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302536.6439105/1‑94 (MQ=60)
                                                                         CGTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCC                      >  SRR4302536.2465807/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302536.4884820/1‑94 (MQ=60)
                                                                          GTCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCA                     >  SRR4302536.6530024/1‑94 (MQ=60)
                                                                           TCCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCAT                    <  SRR4302536.5791231/94‑1 (MQ=60)
                                                                            CCCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATA                   <  SRR4302536.6436242/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302536.2600818/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302536.1480268/94‑1 (MQ=60)
                                                                             CCTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATAT                  <  SRR4302536.3325527/94‑1 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 <  SRR4302536.105158/94‑1 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 >  SRR4302536.6418298/1‑94 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 >  SRR4302536.3395530/1‑94 (MQ=60)
                                                                              CTTCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATT                 <  SRR4302536.3755003/94‑1 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               >  SRR4302536.6145156/1‑94 (MQ=60)
                                                                                TCGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTAT               <  SRR4302536.5014167/94‑1 (MQ=60)
                                                                                 CGCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATG              <  SRR4302536.6504025/94‑1 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             >  SRR4302536.1385438/1‑94 (MQ=60)
                                                                                  GCAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGC             >  SRR4302536.3295030/1‑94 (MQ=60)
                                                                                   CAGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCA            <  SRR4302536.2335616/94‑1 (MQ=60)
                                                                                    AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           >  SRR4302536.5384018/1‑94 (MQ=60)
                                                                                    AGCTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAG           <  SRR4302536.1465091/94‑1 (MQ=60)
                                                                                      CTGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCA         >  SRR4302536.1054416/1‑94 (MQ=60)
                                                                                       TGGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCAC        >  SRR4302536.562480/1‑94 (MQ=60)
                                                                                        GGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGTAGCACG       <  SRR4302536.3859239/94‑1 (MQ=60)
                                                                                        GGCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACG       <  SRR4302536.188190/94‑1 (MQ=60)
                                                                                         GCGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGC      <  SRR4302536.1605757/94‑1 (MQ=60)
                                                                                          CGCGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCC     <  SRR4302536.5121535/94‑1 (MQ=60)
                                                                                            CGCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTT   <  SRR4302536.2602730/94‑1 (MQ=60)
                                                                                             GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  <  SRR4302536.6734306/94‑1 (MQ=60)
                                                                                             GCGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  SRR4302536.5761947/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAATGCTCATGTCGGAGATAGCAGGCAGCGGCGTATTCTCGACATACACGTGGCGCGCTTCCCGACGCAGACGCGTCCCTTCGCAGCTGGCGCACGGACGATTACTGATAAACTTGGCTAATTCTTCGCGTACCGCGCTGGATTCCGTCTCTTTATAGCGGCGCTCCATATTATGCAGCACGCCTTC  >  NC_000913/4270142‑4270328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑