Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG99.8% 1613.8 / NA 410S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (213/196);  minor base C (1/0);  total (214/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009131,741,5750GA99.8% 1590.5 / ‑4.5 410S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (212/196);  minor base C (1/0);  total (214/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 422 total aligned reads displayed.

CAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTC  >  NC_000913/1741484‑1741664
                                                                                          ||                                                                                         
cAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAcc                                                                                       >  1:2448233/1‑96 (MQ=255)
cAGAAGTAAAGCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAcc                                                                                       >  1:1891216/1‑96 (MQ=255)
 aGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                      <  2:7400616/96‑1 (MQ=255)
  gAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                     >  2:1863416/1‑96 (MQ=255)
  gAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                     >  1:2653039/1‑96 (MQ=255)
  gAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                     >  1:4695283/1‑96 (MQ=255)
  gAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                     <  2:2600383/96‑1 (MQ=255)
   aaGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTg                                                                                    >  2:6838608/1‑96 (MQ=255)
     gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                                  <  2:1667612/96‑1 (MQ=255)
     gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                                  <  2:9501455/96‑1 (MQ=255)
     gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                                  >  2:7133121/1‑96 (MQ=255)
      tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                                 >  2:12373082/1‑96 (MQ=255)
      tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                                 <  2:2241048/96‑1 (MQ=255)
      tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                                 <  2:13112714/96‑1 (MQ=255)
        tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATgtc                                                                               <  1:11102028/96‑3 (MQ=255)
        tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggc                                                                               <  1:3435397/96‑1 (MQ=255)
         atCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcg                                                                              <  2:3653983/96‑1 (MQ=255)
         atCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcg                                                                              >  1:8404766/1‑96 (MQ=255)
           cAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                            <  2:5100687/96‑1 (MQ=255)
            aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggc                                                                           <  2:5273689/96‑1 (MQ=255)
            aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggc                                                                           <  2:10683740/96‑1 (MQ=255)
              tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAACTCGCCCAAACCGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                         <  2:1591303/96‑1 (MQ=255)
              tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                         >  1:8637295/1‑96 (MQ=255)
               gAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGggcggc                                                                        >  2:6150329/1‑96 (MQ=255)
                aaGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCt                                                                       >  1:6932120/1‑96 (MQ=255)
                 aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                      >  1:11748830/1‑96 (MQ=255)
                 aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCta                                                                      >  1:5550238/1‑96 (MQ=255)
                    gcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                   >  1:13595003/1‑96 (MQ=255)
                      gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                                 <  2:3053974/96‑1 (MQ=255)
                      gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                                 >  2:5027593/1‑96 (MQ=255)
                       tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGc                                                                >  1:12556846/1‑96 (MQ=255)
                         tattattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCa                                                              >  1:10680951/3‑96 (MQ=255)
                          gTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCag                                                             >  1:508599/1‑95 (MQ=255)
                           ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                            >  2:7796590/1‑96 (MQ=255)
                            tattaGCATTAGCGATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCg                                                           >  1:10962835/1‑96 (MQ=255)
                             attagcattagcAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGGTGGGTTTTGGCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                          <  1:11507024/96‑1 (MQ=255)
                               tagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATCCCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                        <  2:1181282/96‑1 (MQ=255)
                               tagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACa                                                        >  1:1202722/1‑96 (MQ=255)
                                 gcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                      <  2:5679177/96‑1 (MQ=255)
                                 gcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                      <  1:10008210/96‑1 (MQ=255)
                                 gcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATg                                                      <  1:6257031/96‑1 (MQ=255)
                                  cattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGGTATAGTGCCACCGACAggg                                                     >  2:4606323/1‑96 (MQ=255)
                                  cattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                     >  1:7883601/1‑96 (MQ=255)
                                  cattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                     >  2:11620475/1‑96 (MQ=255)
                                    ttagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcg                                                   <  1:1821799/96‑1 (MQ=255)
                                      agcaCTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                                 <  1:8899672/96‑1 (MQ=255)
                                      agcaATCCCGGTGATTCTCTCTCAAATCGCCCAAACTGCGATGGTTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                                 <  2:8997861/96‑1 (MQ=255)
                                      agcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggg                                                 <  1:13788497/96‑2 (MQ=255)
                                       gcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                                <  1:7067845/96‑1 (MQ=255)
                                         aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcggg                                              >  2:14250831/1‑95 (MQ=255)
                                         aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                              <  1:766700/96‑1 (MQ=255)
                                         aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAGggcggccgc                                              >  1:5369441/1‑93 (MQ=255)
                                         aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTACAGTGCCACCGACATGGCGGCGGt                                              <  1:14189266/96‑1 (MQ=255)
                                          aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTTCCAACGACATGGcggggggt                                             >  1:8205666/1‑94 (MQ=255)
                                          aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                             <  1:1959435/96‑1 (MQ=255)
                                           tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                            >  1:12439166/1‑96 (MQ=255)
                                            cccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                           <  1:3109362/96‑1 (MQ=255)
                                             ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCt                                          <  2:467970/96‑1 (MQ=255)
                                              cGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGACGGCCGCTa                                         >  1:4480341/1‑96 (MQ=255)
                                               ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGGTAt                                        >  1:9544756/1‑96 (MQ=255)
                                               ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                        <  1:6118280/96‑1 (MQ=255)
                                               ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCtct                                        >  2:10062977/1‑94 (MQ=255)
                                               ggTGATGCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                        <  1:4105782/96‑1 (MQ=255)
                                                gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGATCGCTATc                                       <  1:12978344/96‑1 (MQ=255)
                                                 tGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                      <  2:9250734/96‑1 (MQ=255)
                                                    ttCTCGCGCAAATCGCCCAAACTGCGATTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCTGCGGTCGCTATCGGTa                                   <  1:1607568/96‑1 (MQ=255)
                                                    ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTa                                   >  1:13300647/1‑96 (MQ=255)
                                                     tctcGCGCAAATCGCCCAAACTGCGATGTGTTTTGTCGATACCGTGATGGCGGGCGGCTATAATGCCACCGACATGGCGGTGGTTGCTATCgggtc                                  >  2:13902440/1‑93 (MQ=255)
                                                     tctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTAc                                  >  1:2880245/1‑96 (MQ=255)
                                                       tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGGTATAGTGCCACCGACATGGCGGCGGTCGGTGTCGGGACtt                                >  1:9309125/1‑96 (MQ=255)
                                                        cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                               >  2:8383360/1‑96 (MQ=255)
                                                         gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCt                              <  1:4807454/96‑1 (MQ=255)
                                                         gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTTTCGGGACTTCt                              >  2:13322595/1‑96 (MQ=255)
                                                           gcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                            >  1:7259968/1‑96 (MQ=255)
                                                            cAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                           >  2:12192176/1‑96 (MQ=255)
                                                            cAAATCGCCCAAACTGCGATGGGTTTTGGCGATACCGTGATGGCGGGCGGCGATAGGGCCACCGACATAGCGGCGGGGGCTATCGGTTCTTCTTTc                           >  1:5799343/1‑96 (MQ=255)
                                                              aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTTTCGGGTCTTCTATCTg                         >  1:6601605/1‑96 (MQ=255)
                                                              aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                         <  2:4427381/96‑1 (MQ=255)
                                                              aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                         <  1:6838608/96‑1 (MQ=255)
                                                                  gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGCTATAGTGCCACCGACATGGCGGGGGTTGCTATCGGTACTTCTTTCTGGCtt                     >  2:6488141/1‑96 (MQ=255)
                                                                  gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                     <  2:2034757/96‑1 (MQ=255)
                                                                  gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTTTCTGGcct                     >  1:4291670/1‑94 (MQ=255)
                                                                   cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTc                    <  2:6291766/96‑1 (MQ=255)
                                                                    ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCCCGATCGGTACTTCTATCTGGCTTcc                   >  2:14183952/1‑96 (MQ=255)
                                                                    ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTTACTGGCTTcc                   >  1:3667140/1‑96 (MQ=255)
                                                                     cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg                  >  2:9650769/1‑96 (MQ=255)
                                                                     cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg                  <  2:3825528/96‑1 (MQ=255)
                                                                      aaaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCgg                 <  1:9706398/96‑1 (MQ=255)
                                                                       aaCTGCGATGGGTTTTGTCCATACCGTGATGGCGGGCGGCTATAGTGCAACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGc                >  2:12765366/1‑96 (MQ=255)
                                                                        aCTGCGATGGGTTTTGTCGATACAGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCggag               <  1:10267149/96‑3 (MQ=255)
                                                                          tGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGGCATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGag             >  1:9121972/1‑95 (MQ=255)
                                                                           gCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc            >  2:10416824/1‑96 (MQ=255)
                                                                             gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCct          <  2:12556846/96‑1 (MQ=255)
                                                                              aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc         <  2:8528470/96‑1 (MQ=255)
                                                                               tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct        >  2:4000893/1‑96 (MQ=255)
                                                                                gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt       <  2:11029870/96‑1 (MQ=255)
                                                                                  gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg     <  2:55610/96‑1 (MQ=255)
                                                                                  gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg     <  1:11427232/96‑1 (MQ=255)
                                                                                   ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCAGCGGTCGCTATCGGGACTTCTATCTGGCTTCCGGCGATCCTCTTTgg    >  2:10313774/1‑96 (MQ=255)
                                                                                     ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTc  <  2:13758655/96‑1 (MQ=255)
                                                                                          ||                                                                                         
CAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTC  >  NC_000913/1741484‑1741664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 237 total aligned reads displayed.
ACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCT  >  NC_000913/1741479‑1741674
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NNNTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                                 <  SRR4302518.11990009/101‑1 (MQ=60)
NNNTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                                 <  SRR4302518.9172994/101‑1 (MQ=60)
NNNTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                                 <  SRR4302518.11577345/101‑1 (MQ=60)
  NNNCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                               <  SRR4302518.7506396/101‑1 (MQ=60)
  GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                               >  SRR4302518.4653235/1‑101 (MQ=60)
   TGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTG                                                                                              >  SRR4302518.2141998/1‑101 (MQ=60)
    GCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGA                                                                                             <  SRR4302518.2530691/101‑1 (MQ=60)
     CAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                                            >  SRR4302518.1906747/1‑101 (MQ=60)
     CAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACAGTGAT                                                                                            >  SRR4302518.4739038/1‑101 (MQ=60)
     CAGAAGTAAAGCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                                            >  SRR4302518.1976801/1‑101 (MQ=60)
      NNNAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           <  SRR4302518.9942797/101‑1 (MQ=60)
      NNNAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           <  SRR4302518.8711262/101‑1 (MQ=60)
      AGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           <  SRR4302518.2042185/101‑1 (MQ=60)
      AGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           >  SRR4302518.2041519/1‑101 (MQ=60)
      AGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           >  SRR4302518.6201831/1‑101 (MQ=60)
      AGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATG                                                                                           <  SRR4302518.485866/101‑1 (MQ=60)
       gcagtatgtcagtgactctCGTCTGTTATTAGCATTAGCAATTCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                                          <  SRR4302518.4380001/82‑1 (MQ=60)
       GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                                          <  SRR4302518.1505039/101‑1 (MQ=60)
       GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGG                                                                                          >  SRR4302518.2779834/1‑101 (MQ=60)
       GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGGTTTGTCGATACCGTGATGG                                                                                          >  SRR4302518.4942019/1‑101 (MQ=60)
        NNNTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGTC                                                                                         <  SRR4302518.11621388/101‑1 (MQ=60)
        NNNTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGAC                                                                                         <  SRR4302518.11991623/101‑1 (MQ=60)
        AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATNNN                                                                                         >  SRR4302518.8851518/1‑101 (MQ=60)
        AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC                                                                                         <  SRR4302518.3595378/101‑1 (MQ=60)
         NNNATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCG                                                                                        <  SRR4302518.14236477/101‑1 (MQ=60)
          NNNTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCTCCCAAACTGCGATGGGTTTTCTCGATACCGTGATCGCGG                                                                                       <  SRR4302518.10621559/101‑1 (MQ=60)
          NNNTATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCTG                                                                                       <  SRR4302518.11318675/101‑1 (MQ=60)
            NNNTCACTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                                     <  SRR4302518.9581495/101‑1 (MQ=60)
            ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                                     <  SRR4302518.5937148/101‑1 (MQ=60)
             TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCG                                                                                    >  SRR4302518.3572079/1‑101 (MQ=60)
             TATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGCCGGNNN                                                                                    >  SRR4302518.13483696/1‑101 (MQ=60)
              ATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGNNN                                                                                   >  SRR4302518.8816826/1‑101 (MQ=60)
               TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                                  <  SRR4302518.3515867/101‑1 (MQ=60)
               TCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCCGC                                                                                  >  SRR4302518.6528561/1‑101 (MQ=60)
               NNNGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGTCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGC                                                                                  <  SRR4302518.14283997/101‑1 (MQ=60)
                NNNTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                                 <  SRR4302518.11904353/101‑1 (MQ=60)
                CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAGATCGCCCAAACAGCGATGGGTTTTGTCTATACTGTGATGGCGGGCcnnn                                                                                 >  SRR4302518.12881614/1‑97 (MQ=60)
                CAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCT                                                                                 >  SRR4302518.5287763/1‑101 (MQ=60)
                CAGTGAAGCGCGTCGGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGCT                                                                                 >  SRR4302518.6538450/1‑101 (MQ=60)
                 NNNGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                                <  SRR4302518.9492622/101‑1 (MQ=60)
                 NNNGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                                <  SRR4302518.13069140/101‑1 (MQ=60)
                 CGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                                <  SRR4302518.5810642/101‑1 (MQ=60)
                 AGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTA                                                                                >  SRR4302518.4366808/1‑101 (MQ=60)
                  NNNAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTTT                                                                               <  SRR4302518.9795733/101‑1 (MQ=60)
                  GTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAT                                                                               <  SRR4302518.597585/101‑1 (MQ=60)
                  GTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAT                                                                               <  SRR4302518.479888/101‑1 (MQ=60)
                   nnnaacgcggCTGTTATTAGCATTAGCAATCCCGGGGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGTTACCGTGATGGCGGGCGGCTATA                                                                              <  SRR4302518.14873269/91‑1 (MQ=60)
                   TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCgnnn                                                                              >  SRR4302518.9057784/1‑97 (MQ=60)
                   TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTNNN                                                                              >  SRR4302518.8437858/1‑101 (MQ=60)
                    NNNGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                             <  SRR4302518.7445420/101‑1 (MQ=60)
                    GAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAG                                                                             >  SRR4302518.881876/1‑101 (MQ=60)
                     AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGT                                                                            <  SRR4302518.3055768/101‑1 (MQ=60)
                     AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGG                                                                            >  SRR4302518.7300113/1‑101 (MQ=60)
                      ATCGCGTCTATTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                           <  SRR4302518.4633188/101‑1 (MQ=60)
                      AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATTGTG                                                                           >  SRR4302518.5832164/1‑101 (MQ=60)
                      AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATANNN                                                                           >  SRR4302518.12304734/1‑101 (MQ=60)
                      AGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                                           >  SRR4302518.7248504/1‑101 (MQ=60)
                        CGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAGAGTNNN                                                                         >  SRR4302518.13483588/1‑101 (MQ=60)
                         NNNTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                                        <  SRR4302518.13094048/101‑1 (MQ=60)
                         GCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGNNN                                                                        >  SRR4302518.14218540/1‑101 (MQ=60)
                          CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCTGCTATAGTGGCAC                                                                       >  SRR4302518.7162438/1‑101 (MQ=60)
                           GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATgcttccacc                                                                      >  SRR4302518.5397443/1‑92 (MQ=60)
                           GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCNNN                                                                      >  SRR4302518.8924177/1‑101 (MQ=60)
                           GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCNNN                                                                      >  SRR4302518.13998257/1‑101 (MQ=60)
                           GTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                                      >  SRR4302518.4447581/1‑101 (MQ=60)
                           CTCTGTTATTAGCATTAGCCATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                                      <  SRR4302518.794971/101‑1 (MQ=60)
                            TCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACGGTGATGGCGGGCGGCTATAGTGCCANNN                                                                     >  SRR4302518.13145958/1‑101 (MQ=60)
                            TCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCG                                                                     <  SRR4302518.797468/101‑1 (MQ=60)
                             CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGA                                                                    <  SRR4302518.1418418/101‑1 (MQ=60)
                             CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGA                                                                    <  SRR4302518.7025140/101‑1 (MQ=60)
                              TATTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCNNN                                                                   >  SRR4302518.11182696/1‑101 (MQ=60)
                              NNNTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATCCCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                                   <  SRR4302518.12264440/101‑1 (MQ=60)
                               TTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACA                                                                  <  SRR4302518.4244519/101‑1 (MQ=60)
                               GTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAGCGACA                                                                  >  SRR4302518.528641/1‑101 (MQ=60)
                               GTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACA                                                                  >  SRR4302518.6662082/1‑101 (MQ=60)
                                TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGANNN                                                                 >  SRR4302518.13643132/1‑101 (MQ=60)
                                TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAT                                                                 >  SRR4302518.4416/1‑101 (MQ=60)
                                 TATTAGCATTAGCGATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACNNN                                                                >  SRR4302518.11476461/1‑101 (MQ=60)
                                 TATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                                <  SRR4302518.6576018/101‑1 (MQ=60)
                                 NNNTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                                <  SRR4302518.14903482/101‑1 (MQ=60)
                                  NNNAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATCG                                                               <  SRR4302518.12040049/101‑1 (MQ=60)
                                   TTAGCATTAGCAATCCCGGTGATTGTCGCGCAAATCGACCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTAgaaggtcaccggcgtgtg                                                              >  SRR4302518.125752/1‑83 (MQ=60)
                                   TTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTTATGTCGGGCGGCTATAGTGCCACCGACATGGG                                                              >  SRR4302518.4738842/1‑101 (MQ=60)
                                    ttaccTTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                             <  SRR4302518.668376/96‑1 (MQ=60)
                                    TAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGGCACCTACATGNNN                                                             >  SRR4302518.11241172/1‑101 (MQ=60)
                                    TAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                             <  SRR4302518.1903661/101‑1 (MQ=60)
                                    TAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                             >  SRR4302518.1251282/1‑101 (MQ=60)
                                    NNNCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                                             <  SRR4302518.10517878/101‑1 (MQ=60)
                                    NNNCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGAG                                                             <  SRR4302518.12395943/101‑1 (MQ=60)
                                     AGCATTAGCAATCGCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGNNN                                                            >  SRR4302518.14041770/1‑101 (MQ=60)
                                      NNNTTAGCACTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                           <  SRR4302518.9330809/101‑1 (MQ=60)
                                      NNNTTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGG                                                           <  SRR4302518.14419743/101‑1 (MQ=60)
                                      NNNTTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                           <  SRR4302518.7486708/101‑1 (MQ=60)
                                      NNNTTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                                           <  SRR4302518.7472478/101‑1 (MQ=60)
                                       TATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCGATATGGCCCCCGACATGGCGNNN                                                          >  SRR4302518.11810293/1‑101 (MQ=60)
                                       CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                                          <  SRR4302518.6176869/101‑1 (MQ=60)
                                        ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGNNN                                                         >  SRR4302518.10350946/1‑101 (MQ=60)
                                         TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGT                                                        <  SRR4302518.796656/101‑1 (MQ=60)
                                          TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                                       <  SRR4302518.2048952/101‑1 (MQ=60)
                                          TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGGGATGGCGGGCGGCTATAGTGCCACCGAGATGGCGGCGGgg                                                       >  SRR4302518.6261492/1‑99 (MQ=60)
                                          NNNCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                                       <  SRR4302518.10172349/101‑1 (MQ=60)
                                           AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGgcc                                                      >  SRR4302518.3760479/1‑98 (MQ=60)
                                           AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCG                                                      >  SRR4302518.1937481/1‑101 (MQ=60)
                                             NNNTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCT                                                    <  SRR4302518.10345171/101‑1 (MQ=60)
                                             CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGC‑GGCGGCGATAGTGCCACCGACATGGCtgcgggcgnnn                                                   >  SRR4302518.10719424/1‑90 (MQ=60)
                                              nnncccggggcTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTA                                                   <  SRR4302518.14764836/90‑1 (MQ=60)
                                              AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAGGGCGGCCGCCGCTA                                                   >  SRR4302518.5644273/1‑101 (MQ=60)
                                               ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTTCCAACGACATGGCGGggggtggnnn                                                  >  SRR4302518.8611167/1‑91 (MQ=60)
                                               ATCCCGGTGATGCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                                  <  SRR4302518.4304704/101‑1 (MQ=60)
                                                TCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCcnnn                                                 >  SRR4302518.13024806/1‑97 (MQ=60)
                                                NNNCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGATCGCTATC                                                 <  SRR4302518.13579794/101‑1 (MQ=60)
                                                 CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGgccgtatcg                                                >  SRR4302518.3223551/1‑92 (MQ=60)
                                                  NNNGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGG                                               <  SRR4302518.14710016/101‑1 (MQ=60)
                                                   CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGgcgccattcgt                                              >  SRR4302518.3251185/1‑90 (MQ=60)
                                                   CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCNNN                                              >  SRR4302518.10945545/1‑101 (MQ=60)
                                                   CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCNNN                                              >  SRR4302518.14508980/1‑101 (MQ=60)
                                                   CGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGACGGCCGCTATCGGG                                              >  SRR4302518.4712940/1‑101 (MQ=60)
                                                   CGGTGATTCTCGCGCAAATCGACCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGTCAACGGCAAGGCGGCGGCCGCTATCNNN                                              >  SRR4302518.8388168/1‑101 (MQ=60)
                                                    GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGGTATCGNNN                                             >  SRR4302518.10006834/1‑101 (MQ=60)
                                                    GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGNNN                                             >  SRR4302518.8944601/1‑101 (MQ=60)
                                                    CGTGATTCTCGCGCAAATCGCCCAAACTGCGATTGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCTGCGGTCGCTATCGGTA                                             <  SRR4302518.1677882/101‑1 (MQ=60)
                                                     GTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGGTATCGGNNN                                            >  SRR4302518.8699495/1‑101 (MQ=60)
                                                      TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTNNN                                           >  SRR4302518.10082092/1‑101 (MQ=60)
                                                      TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACT                                           >  SRR4302518.2698237/1‑101 (MQ=60)
                                                      NNNTTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACT                                           <  SRR4302518.8159829/101‑1 (MQ=60)
                                                       GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTT                                          <  SRR4302518.2835732/101‑1 (MQ=60)
                                                        ATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGATATGGCGGCGGTCGCTATCGGCACTTT                                         >  SRR4302518.5417340/1‑101 (MQ=60)
                                                         TTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                                        <  SRR4302518.5059116/101‑1 (MQ=60)
                                                         TTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                                        <  SRR4302518.5382136/101‑1 (MQ=60)
                                                         TTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTNNN                                        >  SRR4302518.13913268/1‑101 (MQ=60)
                                                          TGTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCAGCGGTCGCTttcggggtctnnn                                       >  SRR4302518.12227031/1‑88 (MQ=60)
                                                          TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCTACCGACATGGCGGGGGTCGCTATCGtgacttnnn                                       >  SRR4302518.12135370/1‑92 (MQ=60)
                                                          TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACCTCTA                                       >  SRR4302518.2751123/1‑101 (MQ=60)
                                                          TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTT                                       >  SRR4302518.3015806/1‑101 (MQ=60)
                                                           CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAT                                      >  SRR4302518.4988584/1‑101 (MQ=60)
                                                            NNNCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAGCGGTACTTCTATC                                     <  SRR4302518.11457457/101‑1 (MQ=60)
                                                              GCGCAGATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTG                                   <  SRR4302518.995860/101‑1 (MQ=60)
                                                              GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTG                                   <  SRR4302518.3638828/101‑1 (MQ=60)
                                                              GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTG                                   <  SRR4302518.7200413/101‑1 (MQ=60)
                                                               CGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTATCTGG                                  >  SRR4302518.5785743/1‑101 (MQ=60)
                                                                GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGGACTTCTATCTNNN                                 >  SRR4302518.7639152/1‑101 (MQ=60)
                                                                GCAAATCGCCCAAACTGCGATGGGCTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAACGACATGGCGGCGGTCGCTATCGGGACTTCGATCTNNN                                 >  SRR4302518.11680705/1‑101 (MQ=60)
                                                                CCAAATCGCCCAAACAGCGATGGGTTTTGTAGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGC                                 <  SRR4302518.2548682/101‑1 (MQ=60)
                                                                 NNNATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                                <  SRR4302518.8405663/101‑1 (MQ=60)
                                                                 CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTATCTGNNN                                >  SRR4302518.7920274/1‑101 (MQ=60)
                                                                 CAAATCGCCCAAACTGCGATGGGTTTTGGCGATACCGTGATGGCGGGCGGCGATAGGGCCACCGACATAGCGGCGGGGGCTATCGGTTCTTCTTTCTTGCT                                >  SRR4302518.6093119/1‑101 (MQ=47)
                                                                  AAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGGACTTCTATCTGGCTT                               >  SRR4302518.5421276/1‑101 (MQ=60)
                                                                   AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTTTCGGGTCTTCTATCTGGCCTC                              >  SRR4302518.6941549/1‑101 (MQ=60)
                                                                    NNNGCCCAAACTGCGATGGTTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCA                             <  SRR4302518.9233192/101‑1 (MQ=60)
                                                                    NNNGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCC                             <  SRR4302518.12371542/101‑1 (MQ=60)
                                                                    ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTNNN                             >  SRR4302518.9400375/1‑101 (MQ=60)
                                                                    ATCGCCCAAACTGCGATGGGGTTTGTCGGTACCGTGATGGCGGGCGGCCATAGTGCCACCGACATGGCGGCGGTCGCgatctggacttctctctcgcgtcc                             >  SRR4302518.2786721/1‑77 (MQ=60)
                                                                     ccaCCCAAACTGAGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                            <  SRR4302518.3878076/98‑1 (MQ=60)
                                                                     TCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTNNN                            >  SRR4302518.12901826/1‑101 (MQ=60)
                                                                     TCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCCATAGTGCCACCGACACGGCGGCGGCCCCTTTCGGTGCTTCTGTCTTGCTTCCG                            >  SRR4302518.2209746/1‑101 (MQ=56)
                                                                     NNNCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                            <  SRR4302518.10869584/101‑1 (MQ=60)
                                                                     NNNCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG                            <  SRR4302518.7402622/101‑1 (MQ=60)
                                                                      NNNCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                           <  SRR4302518.10173306/101‑1 (MQ=60)
                                                                      CGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGG                           <  SRR4302518.2048795/101‑1 (MQ=60)
                                                                       GCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGC                          <  SRR4302518.1770780/101‑1 (MQ=60)
                                                                        NNNAAACTGCGATGGGTTTTGTCGATACCGTGATAGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCG                         <  SRR4302518.14958163/101‑1 (MQ=60)
                                                                        NNNAAACTGCGATGGGTTTTGTCGATACAGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGAG                         <  SRR4302518.10751668/101‑1 (MQ=60)
                                                                        NNNAAACTGCGATGGATTTTGTCGATAATGTGATGGCGCGCGGCTATAGTGCCCCCGACCTGGCGGCGGTCGATATCGGTACTTCTATCTGGCTTCCGGCG                         <  SRR4302518.9933482/101‑1 (MQ=58)
                                                                        CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTCTAGTGCCACCGACATGGCGGCGGTCGCTATCCGGACTTCTATCTGGCTTCCGGCC                         >  SRR4302518.3477883/1‑101 (MQ=60)
                                                                        CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTCTCCGTGCTTCTATCTGGCTGCCGGCG                         >  SRR4302518.6913977/1‑101 (MQ=60)
                                                                         CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCTTACTGGCTTCCTGCGA                        >  SRR4302518.3839115/1‑101 (MQ=60)
                                                                         CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAAGGCGGAGGTCGCTATCGGTACTTCTATCTGGCTTCCGGNNN                        >  SRR4302518.13303593/1‑101 (MQ=60)
                                                                          CAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAT                       >  SRR4302518.1780853/1‑101 (MQ=60)
                                                                          AAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCNNN                       >  SRR4302518.14382757/1‑101 (MQ=60)
                                                                           AAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATC                      <  SRR4302518.643905/101‑1 (MQ=60)
                                                                            AGCTGCGATGGGTTTTGTCGAGACCGTGATGGCGGGCGGCTATAGACTCACCAACATGGCGGCGGTCGCTTTAGGTATTTCTATCTGGtttgcgggcannn                     >  SRR4302518.11604779/1‑88 (MQ=28)
                                                                             ACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCT                    <  SRR4302518.319248/101‑1 (MQ=60)
                                                                             ACTGCGATGGGTTTTGTCGATAACGTGATGGCGGGCGGCTATAATGCCACCGACATGGTGGCGGGCCCTATCGGCACTTCTATCTGGtttctgggtatnnn                    >  SRR4302518.12346736/1‑87 (MQ=52)
                                                                              CTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTC                   <  SRR4302518.5312278/101‑1 (MQ=60)
                                                                               TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGGCATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAgccnnn                  >  SRR4302518.9562272/1‑95 (MQ=60)
                                                                               TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCNNN                  >  SRR4302518.11588728/1‑101 (MQ=60)
                                                                               TGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCCGCGATCCTCT                  >  SRR4302518.7343755/1‑101 (MQ=60)
                                                                                 CGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTTCCGCGATCCTCTTT                >  SRR4302518.459424/1‑101 (MQ=60)
                                                                                 CGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT                <  SRR4302518.2587584/101‑1 (MQ=60)
                                                                                 CGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTATATCTGGCTTCCGGCGATCCccnnn                >  SRR4302518.9727776/1‑96 (MQ=60)
                                                                                  NNNGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG               <  SRR4302518.13780232/101‑1 (MQ=60)
                                                                                  NNNGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG               <  SRR4302518.11962434/101‑1 (MQ=60)
                                                                                  GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGGCATGGCGGCGGTCGCTATCGGGACTTCTATCTGGCTTCCGGCGATCCTCTTTG               >  SRR4302518.4663402/1‑101 (MQ=60)
                                                                                   ATGGGTTTTGTCGCTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTNNN              >  SRR4302518.14515556/1‑101 (MQ=60)
                                                                                    TGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTNNN             >  SRR4302518.12125021/1‑101 (MQ=60)
                                                                                    TGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT             >  SRR4302518.2020452/1‑101 (MQ=60)
                                                                                    NNNGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGT             <  SRR4302518.9894198/101‑1 (MQ=60)
                                                                                     NNNTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGATATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTC            <  SRR4302518.9648825/101‑1 (MQ=60)
                                                                                     GGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGGTCCTCTTTTGTC            >  SRR4302518.729425/1‑101 (MQ=60)
                                                                                       GTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTNNN          >  SRR4302518.13077395/1‑101 (MQ=60)
                                                                                       GTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCAC          <  SRR4302518.914295/101‑1 (MQ=60)
                                                                                       GTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTCCCGGCGATCCTCTTTGGTCAC          <  SRR4302518.3560776/101‑1 (MQ=60)
                                                                                        TTTTGTCGATACCGTGATGTCTGGCGGCTCTAGTGCCACCGACATGGCGGCGGTCtctttctgttcttctacttttgctccgggggttctctttggtccct         >  SRR4302518.4700467/1‑55 (MQ=28)
                                                                                        TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACG         <  SRR4302518.6394585/101‑1 (MQ=60)
                                                                                         TTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTTGTCACGG        >  SRR4302518.4996105/1‑101 (MQ=60)
                                                                                         TTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGG        <  SRR4302518.3407075/101‑1 (MQ=60)
                                                                                         TTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGGACTTCTATCTGGCTTCCGGCGATCCCCTTTGGTCACGG        >  SRR4302518.7272766/1‑101 (MQ=60)
                                                                                          TTGTCGATACCGTAATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGA       <  SRR4302518.1550250/101‑1 (MQ=60)
                                                                                           TGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGAC      <  SRR4302518.2147463/101‑1 (MQ=60)
                                                                                               NNNACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCT  <  SRR4302518.14145680/101‑1 (MQ=60)
                                                                                               |                                                                                                    
ACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCT  >  NC_000913/1741479‑1741674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑