Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,741,574 2 bp→GA coding (94‑95/1374 nt) ydhE → putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,741,5740AG100.0% 601.5 / NA 184S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (101/83);  total (101/83)
*NC_0009131,741,5750GA99.5% 681.0 / NA 184S32D (AGT→GAT) ydhEputative transport protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (100/83);  minor base C (1/0);  total (101/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741488‑1741661
                                                                                      ||                                                                                      
aGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                   >  2:1541267/1‑93 (MQ=255)
aGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                   >  1:5814950/1‑93 (MQ=255)
aGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAAACCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCg                                                                                   >  1:2217991/1‑93 (MQ=255)
 gTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGGTTTGTCGATACCGt                                                                                  >  1:4548999/1‑93 (MQ=255)
   atatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGa                                                                                <  1:1451832/93‑1 (MQ=255)
   atatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGa                                                                                >  1:5353375/1‑93 (MQ=255)
    tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:2261885/93‑1 (MQ=255)
    tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:2703310/93‑1 (MQ=255)
    tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               <  2:4793651/93‑1 (MQ=255)
    tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAt                                                                               >  2:3461817/1‑93 (MQ=255)
         gTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgg                                                                          <  2:6074273/93‑1 (MQ=255)
          tGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcggg                                                                         <  1:3078678/93‑1 (MQ=255)
             aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggggg                                                                      >  2:3898013/1‑93 (MQ=255)
             aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                      >  1:5624419/1‑93 (MQ=255)
             aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggcgg                                                                      >  2:1426803/1‑93 (MQ=255)
             aGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGATTTGTCGATACCGTGATGgcgggcgg                                                                      >  2:1141536/1‑93 (MQ=255)
             aGCGCGTCTGTTATTAGCATTAGCAATCCCGGCGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGgcgggagg                                                                      >  1:6180751/1‑93 (MQ=255)
               cgcgTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCt                                                                    <  2:5767587/93‑1 (MQ=255)
                  gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:6702981/93‑1 (MQ=255)
                  gTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                 <  2:2494867/93‑1 (MQ=255)
                   tCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAg                                                                >  2:2934214/1‑93 (MQ=255)
                     tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  1:3812560/1‑93 (MQ=255)
                     tGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTg                                                              >  2:3035343/1‑93 (MQ=255)
                      gTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCCATAGTGc                                                             >  1:6168211/1‑93 (MQ=255)
                       ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            >  2:4535501/1‑93 (MQ=255)
                       ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                            >  2:6255717/1‑93 (MQ=255)
                       ttattaGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAACGTGATGGCGGGCGGCTATAGTGcc                                                            <  1:6126232/93‑1 (MQ=255)
                         attagcattagcAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAc                                                          <  1:5268752/93‑1 (MQ=255)
                          ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAGCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         >  2:4689802/1‑93 (MQ=255)
                          ttagcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAcc                                                         >  2:3913413/1‑93 (MQ=255)
                            agcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                       >  2:2825070/1‑93 (MQ=255)
                             gcattagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGGCACCGAc                                                      >  1:4655188/1‑93 (MQ=255)
                              cattagcaATCCCAGTGATTCTCGCGCAAATCGCCCAAAAAACGATGAGTTTTGTAGATACCGTGCTGGCGGGCGGCTATAGTGCCACCGACa                                                     <  1:6718731/93‑1 (MQ=255)
                               attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    <  2:5222110/93‑1 (MQ=255)
                               attagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAt                                                    >  2:1277127/1‑93 (MQ=255)
                                 tagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                  <  1:422850/93‑1 (MQ=255)
                                 tagcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                  >  1:2770334/1‑93 (MQ=255)
                                   gcaATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcg                                                <  2:4059844/93‑1 (MQ=255)
                                     aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggg                                              >  2:2018963/1‑92 (MQ=255)
                                     aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                              >  1:1149974/1‑93 (MQ=255)
                                     aaTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcggc                                              <  1:2805849/93‑1 (MQ=255)
                                      aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  1:5690420/1‑93 (MQ=255)
                                      aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  2:2404672/1‑93 (MQ=255)
                                      aTCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                             >  2:5226556/1‑93 (MQ=255)
                                       tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcgg                                            >  1:3714531/1‑93 (MQ=255)
                                       tCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGcggcgg                                            >  2:5326380/1‑93 (MQ=255)
                                         ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          >  2:1593308/1‑93 (MQ=255)
                                         ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  2:5094007/93‑1 (MQ=255)
                                         ccGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTc                                          <  2:4072389/93‑1 (MQ=255)
                                           ggTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGc                                        >  2:3704317/1‑93 (MQ=255)
                                            gTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATATTGCCACCGACATGGCGGCGGGCGCt                                       >  1:657757/1‑93 (MQ=255)
                                             tGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTa                                      <  2:3953605/93‑1 (MQ=255)
                                              gATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAt                                     <  2:6468228/93‑1 (MQ=255)
                                                ttCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                   >  2:4226137/1‑93 (MQ=255)
                                                 tctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCgg                                  <  2:2666571/93‑1 (MQ=255)
                                                  ctcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATAggg                                 >  1:4002695/1‑92 (MQ=255)
                                                   tcGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGCCATGGCGGCGGTCGCTATCGGTa                                >  1:5188460/1‑93 (MQ=255)
                                                    cgcgCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                               <  2:5913867/93‑1 (MQ=255)
                                                     gcgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACt                              <  1:2333298/93‑1 (MQ=255)
                                                      cgcAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACtt                             >  1:874607/1‑93 (MQ=255)
                                                       ccAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTc                            <  1:2349267/92‑1 (MQ=255)
                                                        cAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCt                           >  1:2621902/1‑93 (MQ=255)
                                                        cAAACCGCCCAAGCTGCGATGGGTTTTGTCGATGCCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCt                           <  2:383523/93‑1 (MQ=255)
                                                         aaaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTa                          >  1:4162831/1‑93 (MQ=255)
                                                          aaTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAt                         <  1:1737194/93‑1 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGCTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCCGCGGGCGCTATCGGTACTTCTATc                        >  1:540383/1‑93 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATGCCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  2:3927612/93‑1 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCCCCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  2:853433/93‑1 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTAtt                        >  1:759186/1‑92 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        >  2:5823255/1‑93 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                        <  1:4474073/93‑1 (MQ=255)
                                                           aTCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCGATCGGGACTTCTATc                        >  1:6408011/1‑93 (MQ=255)
                                                            tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       >  1:1284588/1‑93 (MQ=255)
                                                            tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       <  2:550940/93‑1 (MQ=255)
                                                            tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       >  1:473905/1‑93 (MQ=255)
                                                            tCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCt                       >  2:4521465/1‑93 (MQ=255)
                                                              gCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTgg                     >  2:1754814/1‑93 (MQ=255)
                                                               cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    <  2:3217611/93‑1 (MQ=255)
                                                               cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    >  2:2973875/1‑93 (MQ=255)
                                                               cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGc                    <  2:5260879/93‑1 (MQ=255)
                                                               cccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGCCGCTATCGGTACTTCTATCTGGc                    >  1:1670722/1‑93 (MQ=255)
                                                                ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCt                   <  1:4969870/93‑1 (MQ=255)
                                                                ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCt                   >  1:3333754/1‑93 (MQ=255)
                                                                ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCt                   >  2:2291542/1‑93 (MQ=255)
                                                                ccAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCt                   <  2:1336021/93‑1 (MQ=255)
                                                                 cAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                  <  2:2227395/93‑1 (MQ=255)
                                                                  aaaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTc                 <  2:5415952/93‑1 (MQ=255)
                                                                  aaaCTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTc                 <  2:503093/93‑1 (MQ=255)
                                                                       gCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCg            >  2:4579454/1‑93 (MQ=255)
                                                                         gATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAt          >  1:1877744/1‑93 (MQ=255)
                                                                          aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc         >  2:5881519/1‑93 (MQ=255)
                                                                          aTGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATc         <  1:1605618/93‑1 (MQ=255)
                                                                           tGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATcc        <  2:6397976/93‑1 (MQ=255)
                                                                            gggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTGCGGCGATCct       <  1:2083407/93‑1 (MQ=255)
                                                                             ggTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc      <  1:4601552/93‑1 (MQ=255)
                                                                              gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct     <  1:3629795/93‑1 (MQ=255)
                                                                              gTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCCCTATCGGTACTTCTATCTGGCTTCCGGCGATCctcc     >  1:4419849/1‑92 (MQ=255)
                                                                               ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  1:4279207/93‑1 (MQ=255)
                                                                               ttttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCtt    <  2:337235/93‑1 (MQ=255)
                                                                                 ttGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg  <  2:4757313/93‑1 (MQ=255)
                                                                                      ||                                                                                      
AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTG  >  NC_000913/1741488‑1741661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT  >  NC_000913/1741486‑1741660
                                                                                        |                                                                                      
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                   <  SRR4302536.3884082/94‑1 (MQ=60)
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACC                                                                                   <  SRR4302536.1660044/94‑1 (MQ=60)
 AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCG                                                                                  >  SRR4302536.5988171/1‑94 (MQ=60)
 AAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAAACCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCG                                                                                  >  SRR4302536.2285066/1‑94 (MQ=60)
  AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                 >  SRR4302536.3080364/1‑94 (MQ=60)
  AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGT                                                                                 >  SRR4302536.3885492/1‑94 (MQ=60)
  AGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGGTTTGTCGATACCGT                                                                                 >  SRR4302536.4685426/1‑94 (MQ=60)
    TATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGA                                                                               >  SRR4302536.5513157/1‑94 (MQ=60)
     ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                              <  SRR4302536.4789007/94‑1 (MQ=60)
     ATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGAT                                                                              <  SRR4302536.1494838/94‑1 (MQ=60)
            TGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGC                                                                       <  SRR4302536.3168804/94‑1 (MQ=60)
              AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                     >  SRR4302536.3784919/1‑94 (MQ=60)
              AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                     >  SRR4302536.5791492/1‑94 (MQ=60)
              AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGG                                                                     >  SRR4302536.2387429/1‑94 (MQ=60)
              AAGCGCGTCTGTTATTAGCATTAGCAATCCCGGCGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGAGG                                                                     >  SRR4302536.6364839/1‑94 (MQ=60)
                   CGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATA                                                                >  SRR4302536.861064/1‑94 (MQ=60)
                      CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                             <  SRR4302536.542247/94‑1 (MQ=60)
                      CTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTG                                                             >  SRR4302536.3926216/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGC                                                            >  SRR4302536.4076373/1‑94 (MQ=60)
                       TGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCCATAGTGC                                                            >  SRR4302536.6351937/1‑94 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCA                                                          <  SRR4302536.4298974/94‑1 (MQ=60)
                         TTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAACGTGATGGCGGGCGGCTATAGTGCCA                                                          <  SRR4302536.6308809/94‑1 (MQ=60)
                           ATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACC                                                        <  SRR4302536.5425781/94‑1 (MQ=60)
                              AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGGCACCGAC                                                     >  SRR4302536.4795154/1‑94 (MQ=60)
                              AGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGAC                                                     <  SRR4302536.4136465/94‑1 (MQ=60)
                               GCATAAGCAATCCCGGTGGTTCTCGCGCAAATCGCCCAAACTGCGATGGGATTTGTCGATACCGTGATGGCGGGCGGCTATTGTGGCAGCGACA                                                    >  SRR4302536.5953121/1‑94 (MQ=60)
                                CATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACAT                                                   <  SRR4302536.5854083/94‑1 (MQ=60)
                                CATTAGCAATCCCAGTGATTCTCGCGCAAATCGCCCAAAAAACGATGAGTTTTGTAGATACCGTGCTGGCGGGCGGCTATAGTGCCACCGACAT                                                   <  SRR4302536.6918207/94‑1 (MQ=53)
                                 ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                  >  SRR4302536.2119796/1‑94 (MQ=60)
                                 ATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATG                                                  >  SRR4302536.3314691/1‑94 (MQ=60)
                                  TTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGG                                                 >  SRR4302536.2852677/1‑94 (MQ=60)
                                   TAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGC                                                <  SRR4302536.433994/94‑1 (MQ=60)
                                    AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                               >  SRR4302536.6842366/1‑94 (MQ=60)
                                    AGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCG                                               <  SRR4302536.596551/94‑1 (MQ=60)
                                     GCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGG                                              <  SRR4302536.2808014/94‑1 (MQ=60)
                                      CAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGC                                             >  SRR4302536.1184580/1‑94 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                            >  SRR4302536.6330959/1‑94 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                            <  SRR4302536.6510684/94‑1 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                            >  SRR4302536.5859618/1‑94 (MQ=60)
                                       AATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCG                                            <  SRR4302536.2889056/94‑1 (MQ=60)
                                        ATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGG                                           >  SRR4302536.3825576/1‑94 (MQ=60)
                                          CCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTC                                         <  SRR4302536.5875110/94‑1 (MQ=60)
                                             GGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATATTGCCACCGACATGGCGGCGGGCGCT                                      >  SRR4302536.677041/1‑94 (MQ=60)
                                               TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                    <  SRR4302536.1688156/94‑1 (MQ=60)
                                               TGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTAT                                    >  SRR4302536.5311648/1‑94 (MQ=60)
                                                GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                   >  SRR4302536.5515100/1‑94 (MQ=60)
                                                GATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATC                                   <  SRR4302536.4486542/94‑1 (MQ=60)
                                                  TTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGG                                 <  SRR4302536.4382715/94‑1 (MQ=60)
                                                   TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATaggg                                >  SRR4302536.4121465/1‑90 (MQ=60)
                                                   TCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCCATCGGT                                >  SRR4302536.4669147/1‑94 (MQ=60)
                                                    CTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGCCATGGCGGCGGTCGCTATCGGTA                               >  SRR4302536.5343205/1‑94 (MQ=60)
                                                      CGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGGGGCTATAGTGGCACCGACATGGCGGCGGGCGCTATCGGGACT                             >  SRR4302536.2405791/1‑94 (MQ=60)
                                                       GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTT                            >  SRR4302536.900380/1‑94 (MQ=60)
                                                       GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTT                            <  SRR4302536.2404096/94‑1 (MQ=60)
                                                       GCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTT                            <  SRR4302536.2694122/94‑1 (MQ=60)
                                                         GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                          <  SRR4302536.174111/94‑1 (MQ=60)
                                                         GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                          >  SRR4302536.1905003/1‑94 (MQ=60)
                                                         GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGCACTTCT                          >  SRR4302536.5026787/1‑94 (MQ=60)
                                                         GCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGGCGCTATCGGTACTTCT                          >  SRR4302536.2700543/1‑94 (MQ=60)
                                                         CCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCT                          <  SRR4302536.2420484/94‑1 (MQ=60)
                                                          CAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTA                         >  SRR4302536.4286497/1‑94 (MQ=60)
                                                            AATCGCCCAAACTGCGATGGGTTTTGTCGCTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCCGCGGGCGCTATCGGTACTTCTATC                       >  SRR4302536.554175/1‑94 (MQ=60)
                                                            AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATT                       >  SRR4302536.781441/1‑94 (MQ=60)
                                                            AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATC                       <  SRR4302536.1787302/94‑1 (MQ=60)
                                                            AATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCGATCGGGACTTCTATC                       >  SRR4302536.6599400/1‑94 (MQ=60)
                                                             ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                      <  SRR4302536.4608475/94‑1 (MQ=60)
                                                             ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                      >  SRR4302536.1323032/1‑94 (MQ=60)
                                                             ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                      <  SRR4302536.1602215/94‑1 (MQ=60)
                                                             ATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT                      >  SRR4302536.486248/1‑94 (MQ=60)
                                                                GCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGC                   >  SRR4302536.6679169/1‑94 (MQ=60)
                                                                GCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGCCGCTATCGGTACTTCTATCTGGC                   >  SRR4302536.1719182/1‑94 (MQ=60)
                                                                 CCCAAACTGCGATGTGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCGCCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                  <  SRR4302536.4698670/94‑1 (MQ=60)
                                                                 CCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCT                  >  SRR4302536.3432453/1‑94 (MQ=60)
                                                                  CCAAACTGCGATGGTTTTTGTCGATACCGTGATGGCGGTCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                 <  SRR4302536.762902/94‑1 (MQ=60)
                                                                  CCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTT                 <  SRR4302536.5119159/94‑1 (MQ=60)
                                                                     AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCG              >  SRR4302536.5583560/1‑94 (MQ=60)
                                                                     AACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCA              <  SRR4302536.1068936/94‑1 (MQ=60)
                                                                       CTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGC            <  SRR4302536.2878062/94‑1 (MQ=60)
                                                                          CGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGAT         >  SRR4302536.1934245/1‑94 (MQ=60)
                                                                            ATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCC       <  SRR4302536.1652462/94‑1 (MQ=60)
                                                                            ATGGGTTTTGTCGATACCGTGATGGCGGGCGGATATAGTGCCACCGACATGGCGGCGGTCGCTATAGGTACTTCTATCTGGCTTCCGGCGATCC       >  SRR4302536.1481108/1‑94 (MQ=60)
                                                                              GGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTGCGGCGATCCTC     <  SRR4302536.2146112/94‑1 (MQ=60)
                                                                               GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCT    <  SRR4302536.4739742/94‑1 (MQ=60)
                                                                               GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCAGGCGATCCTCT    <  SRR4302536.4854763/94‑1 (MQ=60)
                                                                               GGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCCCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCC    >  SRR4302536.4552200/1‑94 (MQ=60)
                                                                                GTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTT   <  SRR4302536.3738357/94‑1 (MQ=60)
                                                                                 TTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT  <  SRR4302536.4406843/94‑1 (MQ=60)
                                                                                        |                                                                                      
GAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCAGTACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTT  >  NC_000913/1741486‑1741660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑