Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,393,106 Δ1 bp intergenic (‑3443/+857) tldD ← / ← cafA TldD/CafA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,393,1030T.99.5% 2628.6 / 4.6 553intergenic (‑3440/+860)tldD/cafATldD/CafA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base . (297/251);  minor base G (1/2);  total (299/254)
Fisher's exact test for biased strand distribution p-value = 5.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01

Only 100 of 629 total aligned reads displayed.

TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG  >  NC_000913/3393008‑3393194
                                                                                               |                                                                                           
tGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGt                                                                                             >  2:7491727/1‑96 (MQ=255)
   cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttc                                                                                          >  2:11973862/1‑95 (MQ=255)
   cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttc                                                                                          <  1:2161525/96‑2 (MQ=255)
   cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGttcc                                                                                          <  1:11063587/96‑3 (MQ=255)
   cAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGgttc                                                                                          >  2:14361338/1‑95 (MQ=255)
    aGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttca                                                                                         >  2:4094664/1‑94 (MQ=255)
     gCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcag                                                                                        >  2:3824334/1‑93 (MQ=255)
      cccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGtttcagc                                                                                       >  1:3035260/1‑92 (MQ=255)
       ccGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGGTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGcc                                                                                     >  2:12243695/1‑96 (MQ=255)
        cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCa                                                                                    <  2:9719952/96‑1 (MQ=255)
        cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCa                                                                                    >  2:9147404/1‑96 (MQ=255)
        cGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTATCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCa                                                                                    >  1:3710794/1‑96 (MQ=255)
           aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                                 >  1:8798292/1‑96 (MQ=255)
           aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                                 >  1:957266/1‑96 (MQ=255)
           aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                                 <  1:5609435/96‑1 (MQ=255)
           aTTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAc                                                                                 >  2:13231256/1‑96 (MQ=255)
            ttttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACg                                                                                >  2:6066588/1‑96 (MQ=255)
              ttCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTc                                                                              >  2:2664435/1‑96 (MQ=255)
               tCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCa                                                                             <  2:13891776/96‑1 (MQ=255)
                cACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAc                                                                            >  1:1125189/1‑96 (MQ=255)
                 aCGCGTAATATGCGCGGTCAGATTATCCACCGACCGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                           <  1:13734549/96‑1 (MQ=255)
                 aCGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                           <  1:272599/96‑1 (MQ=255)
                 aCGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAcc                                                                           <  1:12231104/96‑1 (MQ=255)
                    cgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgcc                                                                        <  2:12993706/96‑1 (MQ=255)
                    cgTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgcc                                                                        <  2:10954065/96‑1 (MQ=255)
                     gTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAccgccg                                                                       >  2:3073282/1‑96 (MQ=255)
                         tatGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTa                                                                   >  2:2093423/1‑96 (MQ=255)
                            gcggGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAt                                                                >  2:12585383/1‑96 (MQ=255)
                                 gTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCt                                                           <  2:11277365/96‑1 (MQ=255)
                                 gTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCt                                                           >  1:6074740/1‑96 (MQ=255)
                                   cAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCttt                                                         >  2:1784439/1‑96 (MQ=255)
                                     gATTATCCACCGGCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTGCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGt                                                       <  2:7808094/96‑1 (MQ=255)
                                       ttATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCg                                                     >  1:8046236/1‑96 (MQ=255)
                                         aTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAt                                                   >  1:4664241/1‑96 (MQ=255)
                                          tCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATc                                                  <  2:1238918/96‑1 (MQ=255)
                                            cACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGt                                                <  2:2947013/96‑1 (MQ=255)
                                             agcGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTc                                               >  1:11657565/3‑96 (MQ=255)
                                               cGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCAt                                             <  2:10162840/96‑1 (MQ=255)
                                                gaaaGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATc                                            >  1:2546063/4‑96 (MQ=255)
                                                 aCATCGTATACGTTTCCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                           <  1:4354189/96‑1 (MQ=255)
                                                 aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                           <  2:6861343/96‑1 (MQ=255)
                                                 aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                           <  2:5005041/96‑1 (MQ=255)
                                                 aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                           <  2:7702400/96‑1 (MQ=255)
                                                 aCAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATcc                                           >  1:10615283/1‑96 (MQ=255)
                                                  cAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCa                                          >  2:9539812/1‑96 (MQ=255)
                                                  cAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCa                                          >  2:3650472/1‑96 (MQ=255)
                                                   aGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAg                                         >  1:12754814/1‑96 (MQ=255)
                                                    gCGTATGCTTTTCCTCCTCGCTCAACCAGCTGGCTCCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGc                                        <  2:5272671/96‑1 (MQ=255)
                                                    gCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGc                                        >  2:1415821/1‑96 (MQ=255)
                                                     cGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGcc                                       <  1:7605572/96‑1 (MQ=255)
                                                      gTATGCGTTTGCTTCTCGCCCACGCAGCTGGCACCGCCCTG‑TTTCAACCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      <  1:12434895/96‑1 (MQ=255)
                                                      gTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACGGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      >  1:1603586/1‑96 (MQ=255)
                                                      gTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      >  2:11724168/1‑96 (MQ=255)
                                                      gTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      >  2:6698373/1‑96 (MQ=255)
                                                      gTATGCGTGTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      >  1:12971544/1‑96 (MQ=255)
                                                      atATGCGTTTGCTTCTCTCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCt                                      <  1:6507232/95‑1 (MQ=255)
                                                       tATGCGTGTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGccct                                     <  1:10712931/96‑3 (MQ=255)
                                                          gCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCaa                                  <  1:9183215/96‑1 (MQ=255)
                                                           cGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAg                                 >  2:8304195/1‑96 (MQ=255)
                                                           cGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTNCAAg                                 <  2:3363872/96‑1 (MQ=255)
                                                            gTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAgg                                >  2:4463438/1‑96 (MQ=255)
                                                             tttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAggg                               >  1:2473578/1‑96 (MQ=255)
                                                              ttGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGga                              <  1:5824419/96‑1 (MQ=255)
                                                               tGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgag                             <  2:5640865/96‑1 (MQ=255)
                                                                gCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGgaga                            >  2:3241120/1‑96 (MQ=255)
                                                                 catctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGaa                           >  1:9385648/3‑96 (MQ=255)
                                                                 cTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCCCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGaa                           <  2:10622340/96‑1 (MQ=255)
                                                                  ttCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAAc                          >  2:7026870/1‑96 (MQ=255)
                                                                   tctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                         >  1:4458569/1‑96 (MQ=255)
                                                                   gctcGCCCAACCAGCTGGCCCCGCCCTG‑TTTCAGCCAGGCGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACt                         <  1:13543477/95‑1 (MQ=255)
                                                                    ctcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtg                        >  1:7316023/1‑96 (MQ=255)
                                                                     tcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                       >  2:1378823/1‑96 (MQ=255)
                                                                     tcGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACtgt                       <  1:9925079/96‑1 (MQ=255)
                                                                      cGCCCAGCCAGCTGGCCCCGCCCTG‑TTTCAGCCAGCCGTCACCGCCGGTTACATCGCCTTTGTCGCTCGTCATCCAGCCTTCAAGGGAGAACtgtg                      <  1:7083993/96‑1 (MQ=255)
                                                                       gCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCTTCATCCAGCCTTCAAGGGAGAACTGTGc                     >  1:14154882/1‑96 (MQ=255)
                                                                        cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                    <  1:1846908/96‑1 (MQ=255)
                                                                        cccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGcc                    <  1:5288514/96‑1 (MQ=255)
                                                                         ccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCg                   <  2:13591588/96‑1 (MQ=255)
                                                                         ccAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCg                   >  1:8651949/1‑96 (MQ=255)
                                                                          cAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGt                  <  2:9869927/96‑1 (MQ=255)
                                                                           aaCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATGGCCTTTGTCGATCGTAATCCAGCCTTCAAGGGAGAACTGTGCCGtt                 >  1:4957027/1‑96 (MQ=255)
                                                                            aCCAGCTGGCACCG‑CCTGGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGttt                <  1:10317995/96‑1 (MQ=255)
                                                                             ccAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTc               >  1:13400904/1‑96 (MQ=255)
                                                                              cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATGGCCTTTGTCGATCGGCATCCAGCCTTCAAGGGAGCACTGTGCCGTTTca              >  1:4093738/1‑95 (MQ=255)
                                                                              cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc              <  2:2353009/96‑1 (MQ=255)
                                                                              cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc              <  2:9298298/96‑1 (MQ=255)
                                                                              cAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTcc              <  1:3834620/96‑1 (MQ=255)
                                                                               aGCTGGCACCGCCCTG‑TTTCAGCCAGACGCCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCa             <  1:3269778/96‑1 (MQ=255)
                                                                                gCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAg            <  2:6508157/96‑1 (MQ=255)
                                                                                 cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgc           <  2:8798292/96‑1 (MQ=255)
                                                                                 cTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgc           <  1:13118298/96‑1 (MQ=255)
                                                                                  tGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgcg          <  1:5094779/96‑1 (MQ=255)
                                                                                   ggCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTATGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAgcgc         >  1:12100824/1‑96 (MQ=255)
                                                                                      aCCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAat      <  2:14267967/96‑1 (MQ=255)
                                                                                      aCCGCCCTG‑TTTCAGCCAGACATCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAat      >  1:8561763/1‑96 (MQ=255)
                                                                                       ccGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata     <  1:7903662/96‑1 (MQ=255)
                                                                                       ccGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAata     <  1:10008659/96‑1 (MQ=255)
                                                                                        cGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCCGCGCAatat    >  1:6785492/1‑96 (MQ=255)
                                                                                         gCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATAtt   <  1:14309189/96‑1 (MQ=255)
                                                                                          cccTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTg  <  2:3542348/96‑1 (MQ=255)
                                                                                               |                                                                                           
TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG  >  NC_000913/3393008‑3393194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 358 total aligned reads displayed.
ACTGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCAT  >  NC_000913/3393006‑3393206
                                                                                                    |                                                                                                    
ACTGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTC                                                                                                      <  SRR4302518.2264421/101‑1 (MQ=60)
  TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTNNN                                                                                                    >  SRR4302518.14581183/1‑101 (MQ=60)
  TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTNNN                                                                                                    >  SRR4302518.8174912/1‑101 (MQ=60)
  TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                                   <  SRR4302518.2908316/101‑1 (MQ=60)
  TGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAG                                                                                                   >  SRR4302518.4944708/1‑101 (MQ=60)
   GCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTTGCACCGCCCTG‑TTTCAGC                                                                                                  >  SRR4302518.5264914/1‑101 (MQ=60)
     CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCA                                                                                                >  SRR4302518.4602537/1‑101 (MQ=60)
     CAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCA                                                                                                >  SRR4302518.5000181/1‑101 (MQ=60)
      NNNCCGGATTTTCACGCGTAACATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAG                                                                                               <  SRR4302518.13413387/101‑1 (MQ=60)
        CCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCANNN                                                                                             >  SRR4302518.7609270/1‑101 (MQ=60)
        CCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAG                                                                                             >  SRR4302518.3177538/1‑101 (MQ=60)
        CCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGAC                                                                                             <  SRR4302518.5893874/101‑1 (MQ=60)
        CCCGGATTTTCACGCCTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGTC                                                                                             <  SRR4302518.7161806/101‑1 (MQ=60)
         CCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACG                                                                                            <  SRR4302518.778685/101‑1 (MQ=60)
          CGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGANNN                                                                                           >  SRR4302518.10076681/1‑101 (MQ=60)
           nnntgTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTC                                                                                          <  SRR4302518.13555333/96‑1 (MQ=60)
            GATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGNNN                                                                                         >  SRR4302518.7999694/1‑101 (MQ=60)
            GATTATCACGCGTAAGATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACGGCCCTG‑TTTCAGCCAGACacca                                                                                         >  SRR4302518.5630367/1‑97 (MQ=60)
             ATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTNNN                                                                                        >  SRR4302518.9225349/1‑101 (MQ=60)
             ATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCAC                                                                                        >  SRR4302518.634774/1‑101 (MQ=60)
             ATTTTCACGCGTAATATGCGCGGGCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGGACCGCCCTG‑TTTCAGCGATACGTCAC                                                                                        >  SRR4302518.2121924/1‑101 (MQ=60)
              TTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                       <  SRR4302518.283633/101‑1 (MQ=60)
              NNNTCACGCGTAATATGCGCGGTCAGATTATCCACCGACCGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                       <  SRR4302518.14363345/101‑1 (MQ=60)
              NNNTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACC                                                                                       <  SRR4302518.12810424/101‑1 (MQ=60)
                  NNNGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCG                                                                                   <  SRR4302518.12920461/101‑1 (MQ=60)
                  NNNGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCC                                                                                   <  SRR4302518.11798462/101‑1 (MQ=60)
                    CTCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCNNN                                                                                 >  SRR4302518.9439761/1‑101 (MQ=60)
                    CGAGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGT                                                                                 <  SRR4302518.515078/101‑1 (MQ=60)
                     NNNTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCCCCGCCGGTT                                                                                <  SRR4302518.9960379/101‑1 (MQ=60)
                     GCGTAGTATGCGCGGTCAGATTATCCCCCGACAGCGTATGCTTTTGCTTCTCGCCCAACCAGCTGGCCCCGCCCTG‑TTTCAGCCAGACGACACCGCCGGTT                                                                                <  SRR4302518.6157741/101‑1 (MQ=60)
                     GCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTT                                                                                <  SRR4302518.664217/101‑1 (MQ=60)
                      CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTA                                                                               <  SRR4302518.5409698/101‑1 (MQ=60)
                      CGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGNNN                                                                               >  SRR4302518.11704348/1‑101 (MQ=60)
                       GTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTAC                                                                              <  SRR4302518.4196292/101‑1 (MQ=60)
                       GTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTAC                                                                              >  SRR4302518.4636829/1‑101 (MQ=60)
                        TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACA                                                                             >  SRR4302518.3861682/1‑101 (MQ=60)
                        TAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACA                                                                             <  SRR4302518.4908232/101‑1 (MQ=60)
                         AATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCTCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACAT                                                                            <  SRR4302518.4841470/101‑1 (MQ=60)
                         AATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTANNN                                                                            >  SRR4302518.7635538/1‑101 (MQ=60)
                         AATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTANNN                                                                            >  SRR4302518.10027285/1‑101 (MQ=60)
                          NNNTGCGCGGTCAGATTATCCTCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATC                                                                           <  SRR4302518.10815489/101‑1 (MQ=60)
                          NNNTGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATC                                                                           <  SRR4302518.9020047/101‑1 (MQ=60)
                          ATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATC                                                                           >  SRR4302518.2033015/1‑101 (MQ=60)
                          ATATGCGCGGGCAGATTATCCACCGACGGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTCCAGCCAGACGTGACCGCCGGTTACGTC                                                                           >  SRR4302518.1694948/1‑101 (MQ=60)
                          ATATGCGAGGTCTGAATATCCACCGACAGCGTATGCGTCTGCTTCTCGCCCAATCAGCTGGCACCGCCCTG‑TTTCAGACAGACGTCACCGCCGGTTACATC                                                                           <  SRR4302518.966733/101‑1 (MQ=60)
                           TATGCGAGGTCAGATTATCCACCGACAGCCTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGATACATCG                                                                          >  SRR4302518.5708219/1‑101 (MQ=60)
                           NNNGCGCGGTCAGATTATCCACCGACAGAGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCG                                                                          <  SRR4302518.13118423/101‑1 (MQ=60)
                            ATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGC                                                                         >  SRR4302518.3092828/1‑101 (MQ=60)
                              NNNCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCT                                                                       <  SRR4302518.8421793/101‑1 (MQ=60)
                              GCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGNNN                                                                       >  SRR4302518.9298615/1‑101 (MQ=60)
                               cgcactcatgttatcctccgtctGCGTATGCTTTTGCTTCTAGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCCCCGCCGGTTACATCGCCTT                                                                      <  SRR4302518.351025/78‑1 (MQ=60)
                               NNNGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTT                                                                      <  SRR4302518.14180100/101‑1 (MQ=60)
                               NNNGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTT                                                                      <  SRR4302518.11695077/101‑1 (MQ=60)
                                 CGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTG                                                                    >  SRR4302518.2721056/1‑101 (MQ=60)
                                 CGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTNNN                                                                    >  SRR4302518.12749020/1‑101 (MQ=60)
                                 CGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTNNN                                                                    >  SRR4302518.10425313/1‑101 (MQ=60)
                                 CGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTNNN                                                                    >  SRR4302518.9893853/1‑101 (MQ=60)
                                 CGG‑CAGATTATCCACCGCCAGCGTATGCGTTTGCTTCCCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTCCATCGCCTTTGT                                                                   <  SRR4302518.6472336/101‑1 (MQ=60)
                                  NNNCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGT                                                                   <  SRR4302518.13751377/101‑1 (MQ=60)
                                  GGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGC                                                                   >  SRR4302518.1753323/1‑101 (MQ=60)
                                   NNNAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTC                                                                  <  SRR4302518.9758184/101‑1 (MQ=60)
                                   GTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTC                                                                  <  SRR4302518.5474276/101‑1 (MQ=60)
                                   GTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTC                                                                  <  SRR4302518.8934/101‑1 (MQ=60)
                                   GTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTGGTC                                                                  >  SRR4302518.6383086/1‑101 (MQ=60)
                                   GGCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTNNN                                                                  >  SRR4302518.12217314/1‑101 (MQ=60)
                                    TCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGNNN                                                                 >  SRR4302518.9095062/1‑101 (MQ=60)
                                    NNNGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCT                                                                 <  SRR4302518.14436678/101‑1 (MQ=60)
                                     CAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTNNN                                                                >  SRR4302518.14150944/1‑101 (MQ=60)
                                     CAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTNNN                                                                >  SRR4302518.12844594/1‑101 (MQ=60)
                                     CAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGA                                                                >  SRR4302518.4491920/1‑101 (MQ=60)
                                      AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACTTCGCCTTTGTCNNN                                                               >  SRR4302518.13830077/1‑101 (MQ=60)
                                      AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAT                                                               <  SRR4302518.5246634/101‑1 (MQ=60)
                                      AGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGAT                                                               >  SRR4302518.1121213/1‑101 (MQ=60)
                                       GATTATCCACCGACAGCGTATGCTCTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TATCAGCCAGATGACACCGCCGGGTACATCGCCTTTGTCGNNN                                                              >  SRR4302518.12683063/1‑101 (MQ=60)
                                       GATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                              >  SRR4302518.6892912/1‑101 (MQ=60)
                                       GATCATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATC                                                              <  SRR4302518.6046232/101‑1 (MQ=60)
                                        ATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCG                                                             <  SRR4302518.4292949/101‑1 (MQ=60)
                                        ATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGANNN                                                             >  SRR4302518.9277830/1‑101 (MQ=60)
                                        ATTAACCCCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCG                                                             <  SRR4302518.798598/101‑1 (MQ=60)
                                         TTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATNNN                                                            >  SRR4302518.8446751/1‑101 (MQ=60)
                                          TAGCCCCCGACAGCGTATGTGTTTGCTCCTCGCCAACCCAGCTGGCGCCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACACCGCCTTTATCGATCGTC                                                           <  SRR4302518.2597796/101‑1 (MQ=57)
                                          NNNCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTC                                                           <  SRR4302518.8367776/101‑1 (MQ=60)
                                           TTCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCA                                                          <  SRR4302518.3902074/101‑1 (MQ=60)
                                            TCCACCGACAGCGGATGCGTTTGCTTCTCGCCCAACCAGCGGGGACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCTTTTGTCGATAGTCAT                                                         >  SRR4302518.3876722/1‑101 (MQ=60)
                                             NNNCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCACC                                                        <  SRR4302518.12407215/101‑1 (MQ=60)
                                             CCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCNNN                                                        >  SRR4302518.11723878/1‑101 (MQ=60)
                                              CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTGAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCC                                                       >  SRR4302518.1353911/1‑101 (MQ=60)
                                              CACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCANNN                                                       >  SRR4302518.11558610/1‑101 (MQ=60)
                                               CCCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCA                                                      <  SRR4302518.5435855/101‑1 (MQ=60)
                                                NNNACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                                     <  SRR4302518.7773330/101‑1 (MQ=60)
                                                NNNACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                                     <  SRR4302518.15080377/101‑1 (MQ=60)
                                                CGGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAG                                                     >  SRR4302518.3521174/1‑101 (MQ=60)
                                                CCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGTCGATCGTCATCCAT                                                     >  SRR4302518.5915054/1‑101 (MQ=60)
                                                CCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGTCGATCGCCATCCAG                                                     >  SRR4302518.7191405/1‑101 (MQ=60)
                                                 CGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGC                                                    <  SRR4302518.4006784/101‑1 (MQ=60)
                                                  NNNAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                                   <  SRR4302518.7994026/101‑1 (MQ=60)
                                                  GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                                   <  SRR4302518.6247544/101‑1 (MQ=60)
                                                  GACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                                   <  SRR4302518.513198/101‑1 (MQ=60)
                                                  GAAAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCC                                                   >  SRR4302518.2668432/1‑101 (MQ=60)
                                                   ACAGCATATGCGTTTGCTTCTCTCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCT                                                  <  SRR4302518.6840618/101‑1 (MQ=60)
                                                    NNNCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTT                                                 <  SRR4302518.11423997/101‑1 (MQ=60)
                                                    CAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTT                                                 >  SRR4302518.3071442/1‑101 (MQ=60)
                                                     NNNGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTC                                                <  SRR4302518.8901012/101‑1 (MQ=60)
                                                     AGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTC                                                <  SRR4302518.5062153/101‑1 (MQ=60)
                                                      NNNTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                               <  SRR4302518.14909682/101‑1 (MQ=60)
                                                      NNNTATGCATTTGGTTCTCTCCCAACCCGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCA                                               <  SRR4302518.11236091/101‑1 (MQ=60)
                                                       NNNATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAA                                              <  SRR4302518.9625796/101‑1 (MQ=60)
                                                       CGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGCTACATCGCCTTTGTCGATCGTCATCCAGCCTTNNN                                              >  SRR4302518.10019677/1‑101 (MQ=60)
                                                        NNNTGCGTTTGCTTCTCGCCCCACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCCCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCACG                                             <  SRR4302518.11099443/101‑1 (MQ=60)
                                                        NNNTGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAG                                             <  SRR4302518.14324705/101‑1 (MQ=60)
                                                        GTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACGGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAG                                             >  SRR4302518.1673668/1‑101 (MQ=60)
                                                        GTATGCGTGTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCNNN                                             >  SRR4302518.13572777/1‑101 (MQ=60)
                                                         TATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCGTTTGTCGATCGTCATCCAGCCTTCANNN                                            >  SRR4302518.10575021/1‑101 (MQ=60)
                                                         TATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGG                                            <  SRR4302518.2344879/101‑1 (MQ=60)
                                                          nnncttttgaTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACAGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGCG                                           <  SRR4302518.13630091/91‑1 (MQ=60)
                                                          ATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCgagcg                                           >  SRR4302518.2162167/1‑96 (MQ=60)
                                                          ATGCGTTTGCTTCTCGCCCAACCAGATGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCCTCATCCAGCCggcaannn                                           >  SRR4302518.12169744/1‑93 (MQ=60)
                                                           TGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGA                                          <  SRR4302518.6119473/101‑1 (MQ=60)
                                                             CGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGNNN                                        >  SRR4302518.8319493/1‑101 (MQ=60)
                                                              GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGANNN                                       >  SRR4302518.9073379/1‑101 (MQ=60)
                                                              GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAC                                       >  SRR4302518.3976513/1‑101 (MQ=60)
                                                              GTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAA                                       <  SRR4302518.2642158/101‑1 (MQ=60)
                                                              CTTTGCTTCTCGCCCACCCAGCTGCCACCGCCCTG‑TTTCAGCCAGTCGTCACCGCCGGTTCCATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAA                                       <  SRR4302518.6924364/101‑1 (MQ=60)
                                                                TTGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGANNN                                     >  SRR4302518.9195003/1‑101 (MQ=60)
                                                                 TGCTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTG                                    >  SRR4302518.5608926/1‑101 (MQ=60)
                                                                 TGCTTCTCGCCCAACCAGCTGGCACCGCCC‑GGTTTCAGCCAGGCGTCACCGCCGGTTAGATCGCCTTTGTCGATCGTCATCCAGCCTTCGAGGGAGAANNN                                    >  SRR4302518.9789080/1‑101 (MQ=60)
                                                                  NNNTCTCGCCCAACCAGCTGCCCCCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGT                                   <  SRR4302518.10398101/101‑1 (MQ=60)
                                                                   agttaaaatcagCCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTNNN                                  >  SRR4302518.9080785/13‑101 (MQ=60)
                                                                   NNNCTCGCCCAGCCAGCTGGCCCCGCCCTG‑TTTCAGCCAGCCGTCACCGCCGGTTACATCGCCTTTGTCGCTCGTCATCCAGCCTTCAAGGGAGAACTGTG                                  <  SRR4302518.7458005/101‑1 (MQ=60)
                                                                   CTTCTCGCCCAACCAGCTGGCCCCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTG                                  <  SRR4302518.4333983/101‑1 (MQ=60)
                                                                   CTTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTagatcggaagagcgtc                                  >  SRR4302518.1852505/1‑85 (MQ=60)
                                                                   CATCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTNNN                                  >  SRR4302518.9839503/1‑101 (MQ=60)
                                                                    TTCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGC                                 >  SRR4302518.519738/1‑101 (MQ=60)
                                                                    TGCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCGGGCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAATGGAGAACTGNNN                                 >  SRR4302518.12304699/1‑101 (MQ=60)
                                                                     TCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCG                                >  SRR4302518.4688201/1‑101 (MQ=60)
                                                                     TCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCC                                <  SRR4302518.5560070/101‑1 (MQ=60)
                                                                     TCTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCC                                <  SRR4302518.1929953/101‑1 (MQ=60)
                                                                     NNNCGCCCAACCAGCTGGCACCGCCC‑GGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCC                                <  SRR4302518.15096965/101‑1 (MQ=60)
                                                                      ttccCCCAACCAGCTGGCCCCGCCCT‑TTTTCAGCCAGACGGCCCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCG                               <  SRR4302518.1048053/97‑1 (MQ=60)
                                                                      CTCGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCG                               <  SRR4302518.325708/101‑1 (MQ=60)
                                                                      CACGCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGNNN                               >  SRR4302518.11176433/1‑101 (MQ=60)
                                                                         NNNCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTGCAAGGGAGAACTGTGCCGTTT                            <  SRR4302518.13473612/101‑1 (MQ=60)
                                                                         NNNCAACCAGCTGGCACCG‑CCTGGTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTT                            <  SRR4302518.10804039/101‑1 (MQ=60)
                                                                         GCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGNNN                            >  SRR4302518.10282239/1‑101 (MQ=60)
                                                                         GCCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGCGAGAACTGTGCCGTTC                            >  SRR4302518.2129367/1‑101 (MQ=60)
                                                                          CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCCCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTC                           <  SRR4302518.6874788/101‑1 (MQ=60)
                                                                          CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTC                           <  SRR4302518.4768675/101‑1 (MQ=60)
                                                                          CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACGGTGCCGTTTC                           >  SRR4302518.3416103/1‑101 (MQ=60)
                                                                          CCCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGGCATCCAGCCTGCAAGGGAGAACTGTGCCGTNNN                           >  SRR4302518.13107611/1‑101 (MQ=60)
                                                                           NNNACCAGCTGGCCCCGCCCTG‑TTTCAGCCAGACGTCTCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAGCTGTGCCGTTTCC                          <  SRR4302518.14089621/101‑1 (MQ=60)
                                                                           CCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGCACTGTGCCGTTNNN                          >  SRR4302518.13905202/1‑101 (MQ=60)
                                                                           CCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCC                          <  SRR4302518.4016039/101‑1 (MQ=60)
                                                                           CCAACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTNNN                          >  SRR4302518.13238832/1‑101 (MQ=60)
                                                                           CCAACCAGCTGGCACCGCCCTG‑GTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTNNN                          >  SRR4302518.11462308/1‑101 (MQ=60)
                                                                            NNNCCAGCTGTCACCGCCCTG‑TTTCAGCCAGACGTTCCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTTAAGGGAGAACTGTGCCGTTTCCA                         <  SRR4302518.10749381/101‑1 (MQ=60)
                                                                            NNNCCAGCTGGCACCGCCATG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCA                         <  SRR4302518.8261790/101‑1 (MQ=60)
                                                                             NNNCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                        <  SRR4302518.12976610/101‑1 (MQ=60)
                                                                             AACCAGCTGGCACTGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCNNN                        >  SRR4302518.14054118/1‑101 (MQ=60)
                                                                             AACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATGGCCTTTGTCGATCGTAATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAG                        >  SRR4302518.5214897/1‑101 (MQ=60)
                                                                             AACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCTATCGTCATCCAGCCTTCAAGGGGGAACTGAGCCGTTTCCAC                        >  SRR4302518.3204377/1‑101 (MQ=60)
                                                                              NNNAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGC                       <  SRR4302518.14812821/101‑1 (MQ=60)
                                                                              NNNAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGC                       <  SRR4302518.13724635/101‑1 (MQ=60)
                                                                              NNNAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAAC                       <  SRR4302518.11138187/101‑1 (MQ=60)
                                                                              ACCAGCTGGCACCGCCCTG‑TTTCAGCCAGGCGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCNNN                       >  SRR4302518.12642081/1‑101 (MQ=60)
                                                                              ACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCCATGGAGAACTGGGCCGTTTCCAGC                       >  SRR4302518.377672/1‑101 (MQ=60)
                                                                              ACCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGCCATCGAGCGTTCACGGGAGCACTGTGCCGTTTCCAGC                       >  SRR4302518.4258230/1‑101 (MQ=60)
                                                                               NNNGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGTG                      <  SRR4302518.11166369/101‑1 (MQ=60)
                                                                               CCAGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCANNN                      >  SRR4302518.14017091/1‑101 (MQ=60)
                                                                                 AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATTCAGCCTTGAAGGGAGGACTGTGCCGTTTCTAGCNNN                    >  SRR4302518.9065912/1‑101 (MQ=60)
                                                                                 AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCNNN                    >  SRR4302518.14187955/1‑101 (MQ=60)
                                                                                 AGCTGGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCA                    >  SRR4302518.2610176/1‑101 (MQ=60)
                                                                                   NNNGCACCGCCCTG‑TTTCAGCCAGACGTCGCCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAAT                  <  SRR4302518.14986590/101‑1 (MQ=60)
                                                                                     GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATAT                >  SRR4302518.260650/1‑101 (MQ=60)
                                                                                     GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGCTTCCAGCGCAANNN                >  SRR4302518.14779083/1‑101 (MQ=60)
                                                                                     GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGGCATCCAGCCTTCAAGAGAGAACATAGCCGATTCCAGCGCAATAT                >  SRR4302518.1320544/1‑101 (MQ=60)
                                                                                     GGCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTATGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAANNN                >  SRR4302518.12676166/1‑101 (MQ=60)
                                                                                     GGCACCGCCCTG‑GTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGGCGATCGTCATCCAGCATTCAAGGGAGAACTGTGCGGTTTCCAGCGCCATAT                >  SRR4302518.4675864/1‑101 (MQ=60)
                                                                                      GTACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATNNN               >  SRR4302518.7773288/1‑101 (MQ=60)
                                                                                      GCACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATT               <  SRR4302518.4271825/101‑1 (MQ=60)
                                                                                       CACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTG              >  SRR4302518.1482369/1‑101 (MQ=60)
                                                                                       CACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATANNN              >  SRR4302518.13689667/1‑101 (MQ=60)
                                                                                        ACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT             >  SRR4302518.2582568/1‑101 (MQ=60)
                                                                                        ACCGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGT             >  SRR4302518.7200885/1‑101 (MQ=60)
                                                                                        ACCGCCCTG‑TTTCAGCCAGACATCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATNNN             >  SRR4302518.8979166/1‑101 (MQ=60)
                                                                                          CGCCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCCGCGCAATATTGTCC           >  SRR4302518.7141813/1‑101 (MQ=60)
                                                                                            nnnctgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCT          <  SRR4302518.8383103/95‑1 (MQ=60)
                                                                                            nnnctgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCAT          <  SRR4302518.7918910/95‑1 (MQ=60)
                                                                                            CCCTG‑TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG         <  SRR4302518.2459346/101‑1 (MQ=60)
                                                                                             nnntgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG         <  SRR4302518.14134053/96‑1 (MQ=60)
                                                                                             nnntgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTG         <  SRR4302518.11271021/96‑1 (MQ=60)
                                                                                             CCTG‑TTTCAGCCAGACGTCACCGCCGGGTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGC        >  SRR4302518.3335404/1‑101 (MQ=60)
                                                                                              CTG‑TTTCAGCCAGACGTCACCGCCTGTTTCATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCTTGCA       <  SRR4302518.2405381/101‑1 (MQ=60)
                                                                                                tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTTCAGCGCAATATTGTCCTGNNN      >  SRR4302518.13372448/3‑101 (MQ=60)
                                                                                                tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCAT      >  SRR4302518.2337340/3‑101 (MQ=60)
                                                                                                tgTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCAC      >  SRR4302518.643686/3‑101 (MQ=60)
                                                                                                 GTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCNNN     >  SRR4302518.10849285/1‑101 (MQ=60)
                                                                                                 GTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATC     <  SRR4302518.122301/101‑1 (MQ=60)
                                                                                                  TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCC    <  SRR4302518.3243093/101‑1 (MQ=60)
                                                                                                  TTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCC    <  SRR4302518.2791657/101‑1 (MQ=60)
                                                                                                   TTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATNNN   >  SRR4302518.11700379/1‑101 (MQ=60)
                                                                                                    |                                                                                                    
ACTGCCAGCCCGGATTTTCACGCGTAATATGCGCGGTCAGATTATCCACCGACAGCGTATGCGTTTGCTTCTCGCCCAACCAGCTGGCACCGCCCTGTTTTCAGCCAGACGTCACCGCCGGTTACATCGCCTTTGTCGATCGTCATCCAGCCTTCAAGGGAGAACTGTGCCGTTTCCAGCGCAATATTGTCCTGCATCCAT  >  NC_000913/3393006‑3393206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑