Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,736,520 A→G Q218R (CAG→CGG)  purR → PurR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,736,5200AG99.5% 667.5 / NA 205Q218R (CAG→CGG) purRPurR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (100/104);  total (101/104)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGG  >  NC_000913/1736430‑1736607
                                                                                          |                                                                                       
tGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCggg                                                                                       <  1:2820690/93‑1 (MQ=255)
tGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCggg                                                                                       >  2:2843627/1‑93 (MQ=255)
tGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCggg                                                                                       >  1:6099521/1‑93 (MQ=255)
   aaCGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTg                                                                                    <  1:356012/93‑1 (MQ=255)
     cGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGAc                                                                                  <  2:410183/93‑1 (MQ=255)
     cGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGAc                                                                                  <  2:4350701/93‑1 (MQ=255)
       tAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTTGATTGTGCGGGGTGACtt                                                                                <  1:5831731/93‑1 (MQ=255)
       tAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGAGtt                                                                                >  2:4778449/1‑93 (MQ=255)
        aaCACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACttt                                                                               <  1:4517230/93‑1 (MQ=255)
         acacCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTg                                                                              >  2:6440069/1‑93 (MQ=255)
           acCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGaa                                                                            <  2:205842/93‑1 (MQ=255)
            ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGGGACTTTGAAc                                                                           >  1:3018909/1‑93 (MQ=255)
             cGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAcc                                                                          <  2:4111711/93‑1 (MQ=255)
                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATGAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                   <  2:1822767/93‑1 (MQ=255)
                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                   >  1:2242561/1‑93 (MQ=255)
                    ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATcc                                                                   <  1:5067042/93‑1 (MQ=255)
                     gccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCg                                                                  >  2:2474267/1‑93 (MQ=255)
                     gccgccTTGCAGGTATTATGAAGGCGATGGAAGAAGCGATGAGCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCg                                                                  <  2:2748930/93‑1 (MQ=255)
                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                 <  1:2835616/93‑1 (MQ=255)
                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                 >  1:187359/1‑93 (MQ=255)
                      ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCgg                                                                 <  1:6624625/93‑1 (MQ=255)
                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                            <  1:2611995/93‑1 (MQ=255)
                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                            <  1:5948363/93‑1 (MQ=255)
                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                            >  1:1620863/1‑93 (MQ=255)
                           ttGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATc                                                            >  1:6357229/1‑93 (MQ=255)
                            tGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATcg                                                           <  2:6112966/93‑1 (MQ=255)
                             gCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATcgc                                                          >  2:6042529/1‑93 (MQ=255)
                             gCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATcgc                                                          <  2:6609285/93‑1 (MQ=255)
                                 gTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCa                                                      <  1:3210534/93‑1 (MQ=255)
                                  ttttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAt                                                     >  1:1745226/1‑93 (MQ=255)
                                   tttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATg                                                    >  2:3572435/1‑93 (MQ=255)
                                   tttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATg                                                    >  2:6325656/1‑93 (MQ=255)
                                    ttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgc                                                   >  2:1058356/1‑93 (MQ=255)
                                    ttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgc                                                   >  1:4236268/1‑93 (MQ=255)
                                    ttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgc                                                   >  1:6255629/1‑93 (MQ=255)
                                     tATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgca                                                  <  2:2273121/93‑1 (MQ=255)
                                       tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcagc                                                <  1:68842/93‑1 (MQ=255)
                                       tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATgcagc                                                >  1:5421037/1‑93 (MQ=255)
                                        gAAGGCGTGGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGCCTTTGAACCTGAATCCGGTTATCGCGCCCTgcagca                                               >  1:3538648/1‑93 (MQ=255)
                                         aaGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaa                                              >  1:5772859/1‑93 (MQ=255)
                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                             >  1:6016567/1‑93 (MQ=255)
                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                             >  2:553444/1‑93 (MQ=255)
                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCaaa                                             >  2:423541/1‑93 (MQ=255)
                                          aGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCCGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGGCATGGAGCaaa                                             <  1:2069211/93‑1 (MQ=255)
                                           ggCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAt                                            >  1:1435875/1‑93 (MQ=255)
                                           ggCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAt                                            <  1:4871919/93‑1 (MQ=255)
                                             cGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATcc                                          <  1:5870506/93‑1 (MQ=255)
                                              gATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCt                                         <  1:4161469/93‑1 (MQ=255)
                                               aTGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTg                                        >  2:3224301/1‑93 (MQ=255)
                                                tGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGt                                       >  2:162784/1‑93 (MQ=255)
                                                tGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTAGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGt                                       <  2:1541152/93‑1 (MQ=255)
                                                 ggAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAGCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTc                                      <  2:3285481/93‑1 (MQ=255)
                                                  gaagaaGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCg                                     >  1:3255209/1‑93 (MQ=255)
                                                   aagaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGc                                    <  1:2543253/93‑1 (MQ=255)
                                                   aagaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGc                                    <  2:1101448/93‑1 (MQ=255)
                                                   aagaagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGc                                    <  1:3305980/93‑1 (MQ=255)
                                                      aagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGc                                 <  1:4323964/93‑1 (MQ=255)
                                                      aagCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGc                                 <  1:1059857/93‑1 (MQ=255)
                                                       agCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGcc                                <  2:1581756/93‑1 (MQ=255)
                                                       agCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGcc                                >  1:117815/1‑93 (MQ=255)
                                                       agCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGcc                                >  1:61424/1‑93 (MQ=255)
                                                       agCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGcc                                >  2:3861244/1‑93 (MQ=255)
                                                         cGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGc                              <  1:551898/93‑1 (MQ=255)
                                                            tgatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATc                           >  2:257777/1‑93 (MQ=255)
                                                             gatCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCg                          >  1:5689461/1‑93 (MQ=255)
                                                              atCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGc                         >  1:922913/1‑93 (MQ=255)
                                                               tCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                        >  2:775184/1‑93 (MQ=255)
                                                               tCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                        >  2:4914223/1‑93 (MQ=255)
                                                               tCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                        >  2:4676113/1‑93 (MQ=255)
                                                               tCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                        >  2:2133674/1‑93 (MQ=255)
                                                                cAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGccc                       >  2:4397843/1‑93 (MQ=255)
                                                                cAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGccc                       >  2:5778096/1‑93 (MQ=255)
                                                                 aaGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCt                      <  2:1223005/93‑1 (MQ=255)
                                                                  aGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTa                     >  1:1289387/1‑93 (MQ=255)
                                                                  aGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTa                     >  1:6720378/1‑93 (MQ=255)
                                                                  aGGTGCCGGAAAGCTGGATTGTGCGGGGCGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAGATCCTGTCGCAGCCGCATCGCCCTa                     >  2:6479165/1‑93 (MQ=255)
                                                                  aGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTa                     >  2:2998418/1‑93 (MQ=255)
                                                                   ggTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAc                    <  2:2628160/93‑1 (MQ=255)
                                                                   ggTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAc                    <  1:3754759/93‑1 (MQ=255)
                                                                      gCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGc                 <  2:2133114/93‑1 (MQ=255)
                                                                       ccGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                <  1:6042302/93‑1 (MQ=255)
                                                                          gAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtc             <  2:5938478/93‑1 (MQ=255)
                                                                           aaaGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtct            <  2:152890/93‑1 (MQ=255)
                                                                           aaaGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtct            <  1:2304683/93‑1 (MQ=255)
                                                                            aaGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtctt           <  1:1966564/93‑1 (MQ=255)
                                                                             aGCTGGATTGTGCGGTGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc          <  2:6696810/93‑1 (MQ=255)
                                                                             aGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttc          <  2:3475624/93‑1 (MQ=255)
                                                                              gCTGGATTGTGCGGGGTGACTTTGACCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTATCGCAGCCGCATCGCCCTACTGCCGtcttct         <  1:1671363/93‑1 (MQ=255)
                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct         >  1:3049439/1‑93 (MQ=255)
                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct         <  2:2819372/93‑1 (MQ=255)
                                                                              gCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtcttct         >  2:3713284/1‑93 (MQ=255)
                                                                               cTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtg        <  1:2330243/93‑1 (MQ=255)
                                                                               cTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtg        <  1:4060274/93‑1 (MQ=255)
                                                                                tGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtgt       >  2:1998044/1‑93 (MQ=255)
                                                                                tGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtgt       <  2:341841/93‑1 (MQ=255)
                                                                                  gATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtgg     >  2:172854/1‑93 (MQ=255)
                                                                                   aTTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggt    >  1:3915430/1‑93 (MQ=255)
                                                                                   aTTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggt    >  2:6215834/1‑93 (MQ=255)
                                                                                   aTTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggt    <  1:1933512/93‑1 (MQ=255)
                                                                                   aTTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggg    >  2:5273425/1‑92 (MQ=255)
                                                                                          |                                                                                       
TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGG  >  NC_000913/1736430‑1736607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGAT  >  NC_000913/1736427‑1736611
                                                                                             |                                                                                           
CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCG                                                                                             >  SRR4302536.2089227/1‑94 (MQ=60)
CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCG                                                                                             <  SRR4302536.3485042/94‑1 (MQ=60)
CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCG                                                                                             <  SRR4302536.4542730/94‑1 (MQ=60)
  CTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGG                                                                                           >  SRR4302536.6281385/1‑94 (MQ=60)
  CTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGG                                                                                           <  SRR4302536.5324024/94‑1 (MQ=60)
   TGGAACGTATCACCGGCGCAGCCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                          <  SRR4302536.409510/94‑1 (MQ=60)
   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                          >  SRR4302536.52674/1‑94 (MQ=60)
   TGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGG                                                                                          <  SRR4302536.2904231/94‑1 (MQ=60)
     GAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTG                                                                                        <  SRR4302536.2577538/94‑1 (MQ=60)
     GAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCAGGAAAGCTGGATTGTGCGGGGTG                                                                                        <  SRR4302536.5865146/94‑1 (MQ=60)
      AACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGA                                                                                       <  SRR4302536.365437/94‑1 (MQ=60)
        CGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACT                                                                                     <  SRR4302536.5053274/94‑1 (MQ=60)
         GTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTT                                                                                    >  SRR4302536.2911369/1‑94 (MQ=60)
         GTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTT                                                                                    <  SRR4302536.322012/94‑1 (MQ=60)
          TAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTTGATTGTGCGGGGTGACTTT                                                                                   <  SRR4302536.6005493/94‑1 (MQ=60)
           AACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTG                                                                                  <  SRR4302536.4652721/94‑1 (MQ=60)
           AACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTG                                                                                  <  SRR4302536.2126286/94‑1 (MQ=60)
            ACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGGGACTTTGA                                                                                 >  SRR4302536.1532051/1‑94 (MQ=60)
              ACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAC                                                                               <  SRR4302536.6747593/94‑1 (MQ=60)
              ACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAC                                                                               <  SRR4302536.6747594/94‑1 (MQ=60)
              ACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAC                                                                               >  SRR4302536.2356872/1‑94 (MQ=60)
              ACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAAC                                                                               <  SRR4302536.5655033/94‑1 (MQ=60)
              ACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGGGACTTTGAAC                                                                               >  SRR4302536.3107623/1‑94 (MQ=60)
                   CGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACATGAA                                                                          >  SRR4302536.6209510/1‑94 (MQ=60)
                     CAGCCCGCCTTGCGGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATC                                                                        <  SRR4302536.1801740/94‑1 (MQ=60)
                      AGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCC                                                                       >  SRR4302536.2310425/1‑94 (MQ=60)
                      AGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCC                                                                       >  SRR4302536.5447016/1‑94 (MQ=60)
                       GGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCG                                                                      <  SRR4302536.5218781/94‑1 (MQ=60)
                       GGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCG                                                                      <  SRR4302536.6328688/94‑1 (MQ=60)
                        GCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGG                                                                     >  SRR4302536.192300/1‑94 (MQ=60)
                         CCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGT                                                                    <  SRR4302536.2919537/94‑1 (MQ=60)
                         CCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGT                                                                    <  SRR4302536.6821669/94‑1 (MQ=60)
                            CCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTTTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                 <  SRR4302536.4404965/94‑1 (MQ=60)
                            ACTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTAT                                                                 >  SRR4302536.6907535/1‑94 (MQ=60)
                             CTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATC                                                                >  SRR4302536.6546620/1‑94 (MQ=60)
                             CTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATC                                                                >  SRR4302536.1668076/1‑94 (MQ=60)
                              TTGCCGGTTTTATGAAGGCGATGGCAGAAGCGATGATCAAGGGGCCGGAAAGCTGGATTGAGCGAGGTGACTTTGATCATGAATCCGGTTATgc                                                               >  SRR4302536.2285480/1‑92 (MQ=60)
                              TTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCG                                                               <  SRR4302536.2690371/94‑1 (MQ=60)
                              TTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCG                                                               <  SRR4302536.6125571/94‑1 (MQ=60)
                                  CGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCC                                                           >  SRR4302536.6114882/1‑94 (MQ=60)
                                    GTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAT                                                         >  SRR4302536.1795893/1‑94 (MQ=60)
                                    GTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAT                                                         <  SRR4302536.3304703/94‑1 (MQ=60)
                                     TTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATG                                                        >  SRR4302536.3665578/1‑94 (MQ=60)
                                     TTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATG                                                        <  SRR4302536.924816/94‑1 (MQ=60)
                                     TTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATG                                                        <  SRR4302536.1861412/94‑1 (MQ=60)
                                      TTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGC                                                       >  SRR4302536.4362366/1‑94 (MQ=60)
                                      TTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGC                                                       >  SRR4302536.6441994/1‑94 (MQ=60)
                                       TTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCA                                                      <  SRR4302536.1937284/94‑1 (MQ=60)
                                        TATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAG                                                     <  SRR4302536.6060058/94‑1 (MQ=60)
                                         ATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGC                                                    >  SRR4302536.5583084/1‑94 (MQ=60)
                                          TGAAGGCGTGGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGCCTTTGAACCTGAATCCGGTTATCGCGCCCTGCAGCA                                                   >  SRR4302536.3644441/1‑94 (MQ=60)
                                          TGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCA                                                   <  SRR4302536.70712/94‑1 (MQ=60)
                                          TGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCA                                                   >  SRR4302536.6321167/1‑94 (MQ=60)
                                           GAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAA                                                  >  SRR4302536.5944637/1‑94 (MQ=60)
                                            AAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAA                                                 >  SRR4302536.6195944/1‑94 (MQ=60)
                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAT                                                <  SRR4302536.3186977/94‑1 (MQ=60)
                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAT                                                <  SRR4302536.3385836/94‑1 (MQ=60)
                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAT                                                >  SRR4302536.1478476/1‑94 (MQ=60)
                                             AGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCCGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGGCATGGAGCAAAT                                                <  SRR4302536.2131542/94‑1 (MQ=60)
                                              GGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATC                                               >  SRR4302536.3404501/1‑94 (MQ=60)
                                              GGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATC                                               <  SRR4302536.5018497/94‑1 (MQ=60)
                                               GCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCC                                              <  SRR4302536.3138323/94‑1 (MQ=60)
                                                CGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCT                                             <  SRR4302536.6193791/94‑1 (MQ=60)
                                                CGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCT                                             <  SRR4302536.6045428/94‑1 (MQ=60)
                                                 GATGGAAGAAGCGATGATCAAGTTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                            <  SRR4302536.4301339/94‑1 (MQ=60)
                                                 GATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                            <  SRR4302536.6226368/94‑1 (MQ=60)
                                                 GATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                            <  SRR4302536.5788951/94‑1 (MQ=60)
                                                 GATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                            <  SRR4302536.4285062/94‑1 (MQ=60)
                                                 GATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTG                                            >  SRR4302536.1696579/1‑94 (MQ=60)
                                                   TGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTC                                          >  SRR4302536.3740130/1‑94 (MQ=60)
                                                    GGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCG                                         >  SRR4302536.3350642/1‑94 (MQ=60)
                                                      AAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCA                                       <  SRR4302536.2619862/94‑1 (MQ=60)
                                                      AAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCA                                       >  SRR4302536.331660/1‑94 (MQ=60)
                                                      AAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCA                                       <  SRR4302536.3403573/94‑1 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    >  SRR4302536.2923090/1‑94 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    <  SRR4302536.4453449/94‑1 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    >  SRR4302536.63113/1‑94 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    <  SRR4302536.1091553/94‑1 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    >  SRR4302536.120914/1‑94 (MQ=60)
                                                         AAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCC                                    >  SRR4302536.120913/1‑94 (MQ=60)
                                                          AGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCG                                   >  SRR4302536.2021045/1‑94 (MQ=60)
                                                            CGATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCA                                 <  SRR4302536.565964/94‑1 (MQ=60)
                                                             GATGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCAT                                <  SRR4302536.1760468/94‑1 (MQ=60)
                                                               TGATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCG                              >  SRR4302536.5858631/1‑94 (MQ=60)
                                                                GATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCATCCGCATCGC                             >  SRR4302536.1141133/1‑94 (MQ=60)
                                                                GATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGC                             >  SRR4302536.6921253/1‑94 (MQ=60)
                                                                GATCAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGC                             >  SRR4302536.950103/1‑94 (MQ=60)
                                                                   CAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCT                          >  SRR4302536.4927346/1‑94 (MQ=60)
                                                                   CAAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCT                          <  SRR4302536.2375890/94‑1 (MQ=60)
                                                                    AAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTA                         >  SRR4302536.2260715/1‑94 (MQ=60)
                                                                    AAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTA                         <  SRR4302536.4254892/94‑1 (MQ=60)
                                                                    AAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTA                         >  SRR4302536.6919925/1‑94 (MQ=60)
                                                                    AAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTA                         >  SRR4302536.1327954/1‑94 (MQ=60)
                                                                    AAGGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCCGTCGCAGCCGCATCGCCCTA                         >  SRR4302536.3393500/1‑94 (MQ=60)
                                                                      GGTGCCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACT                       <  SRR4302536.3866930/94‑1 (MQ=60)
                                                                          CCGGAAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCG                   <  SRR4302536.6222512/94‑1 (MQ=60)
                                                                              AAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTT               <  SRR4302536.2374617/94‑1 (MQ=60)
                                                                              AAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCACCCGCATCGCCCTACTGCCGTCTT               >  SRR4302536.1329727/1‑94 (MQ=60)
                                                                              AAAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGGTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTT               >  SRR4302536.5985338/1‑94 (MQ=60)
                                                                               AAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTC              >  SRR4302536.4016734/1‑94 (MQ=60)
                                                                               AAGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTC              <  SRR4302536.2025694/94‑1 (MQ=60)
                                                                                AGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCT             >  SRR4302536.3139005/1‑94 (MQ=60)
                                                                                AGCTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCT             <  SRR4302536.4566555/94‑1 (MQ=60)
                                                                                 GCTGGATTGTGCGGGGTGACTTTGACCCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTATCGCAGCCGCATCGCCCTACTGCCGTCTTCTG            <  SRR4302536.1719840/94‑1 (MQ=60)
                                                                                  CTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGT           <  SRR4302536.4180624/94‑1 (MQ=60)
                                                                                  CTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGT           <  SRR4302536.4180625/94‑1 (MQ=60)
                                                                                  CTGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGT           <  SRR4302536.2400950/94‑1 (MQ=60)
                                                                                   TGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTG          <  SRR4302536.601566/94‑1 (MQ=60)
                                                                                   TGGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTG          <  SRR4302536.255514/94‑1 (MQ=60)
                                                                                    GGATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGG         <  SRR4302536.2669675/94‑1 (MQ=60)
                                                                                     GATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGT        <  SRR4302536.2714258/94‑1 (MQ=60)
                                                                                     GATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGT        >  SRR4302536.4031827/1‑94 (MQ=60)
                                                                                      ATTGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTG       <  SRR4302536.1991657/94‑1 (MQ=60)
                                                                                        TGTGCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGC     <  SRR4302536.6472195/94‑1 (MQ=60)
                                                                                           GCGGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGAT  <  SRR4302536.9442/94‑1 (MQ=60)
                                                                                             |                                                                                           
CGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGAT  >  NC_000913/1736427‑1736611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑