Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,768,647 T→C A12A (GCT→GCC)  b1689 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,768,6470TC100.0% 709.6 / NA 214A12A (GCT→GCC) b1689hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (128/86);  total (128/86)

Only 100 of 215 total aligned reads displayed.

GATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTG  >  NC_000913/1768557‑1768736
                                                                                          |                                                                                         
gATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTa                                                                                         >  2:5694045/1‑93 (MQ=255)
gATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTa                                                                                         >  1:4988428/1‑93 (MQ=255)
  tACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATg                                                                                       >  1:2720929/1‑93 (MQ=255)
   aCTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGa                                                                                      >  1:2819627/1‑93 (MQ=255)
    cTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGaa                                                                                     >  1:4178322/1‑93 (MQ=255)
     tgtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAc                                                                                    <  2:1307192/93‑1 (MQ=255)
     tgtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAc                                                                                    <  2:4160086/93‑1 (MQ=255)
     tgtgACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAc                                                                                    >  1:1999361/1‑93 (MQ=255)
         aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                                >  1:2045489/1‑93 (MQ=255)
         aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                                <  2:3394713/93‑1 (MQ=255)
         aCTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCa                                                                                <  2:1603352/93‑1 (MQ=255)
           tctctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAgg                                                                              <  2:657577/93‑1 (MQ=255)
              ctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAc                                                                           <  1:5323071/93‑1 (MQ=255)
              ctTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCAc                                                                           <  1:5885059/93‑1 (MQ=255)
               ttCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACt                                                                          >  1:830042/1‑93 (MQ=255)
                tCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTc                                                                         >  1:4350297/1‑93 (MQ=255)
                tCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTc                                                                         <  2:2373502/93‑1 (MQ=255)
                 cAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTcc                                                                        >  2:5926922/1‑93 (MQ=255)
                 cAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTcc                                                                        <  1:3619973/93‑1 (MQ=255)
                    gCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGcc                                                                     <  2:6038235/93‑1 (MQ=255)
                     ccGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCa                                                                    >  1:6112272/1‑93 (MQ=255)
                       gTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCata                                                                  >  1:5445426/1‑93 (MQ=255)
                       gTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCata                                                                  <  2:6110108/93‑1 (MQ=255)
                        tCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCatat                                                                 >  1:3267453/1‑93 (MQ=255)
                          agcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttt                                                               >  1:4727318/1‑93 (MQ=255)
                          agcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttt                                                               >  2:1911578/1‑93 (MQ=255)
                          agcagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttt                                                               >  1:2370765/1‑93 (MQ=255)
                          agcagGCTGATAATGTTATTTATATTTTATTGTTTATTGCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACGCCGCCATAttt                                                               <  1:6419676/93‑1 (MQ=255)
                            cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttttt                                                             <  1:6017383/93‑1 (MQ=255)
                            cagGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAttttt                                                             <  1:954450/93‑1 (MQ=255)
                             agGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTg                                                            >  1:5651154/1‑93 (MQ=255)
                              ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                           >  1:1346181/1‑93 (MQ=255)
                              ggCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGc                                                           >  2:342529/1‑93 (MQ=255)
                               gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGcc                                                          <  1:4696051/93‑1 (MQ=255)
                               gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGcc                                                          <  2:1728165/93‑1 (MQ=255)
                               gCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGcc                                                          >  2:676680/1‑93 (MQ=255)
                                 tGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCAt                                                        >  1:3831306/1‑93 (MQ=255)
                                   aTAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGa                                                      <  1:1763135/93‑1 (MQ=255)
                                    tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc                                                     >  1:4055155/1‑93 (MQ=255)
                                    tAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAc                                                     <  1:5962604/93‑1 (MQ=255)
                                      aTGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTa                                                   >  1:2167314/1‑93 (MQ=255)
                                       tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                  >  2:6019079/1‑93 (MQ=255)
                                       tGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAt                                                  >  1:6288331/1‑93 (MQ=255)
                                        gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt                                                 <  1:151257/93‑1 (MQ=255)
                                        gTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAtt                                                 <  2:2151001/93‑1 (MQ=255)
                                         ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCCGGCACTCCGCCATATTTTTGCCATTACTATtg                                                >  2:1395974/1‑93 (MQ=255)
                                         ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg                                                >  2:2812459/1‑93 (MQ=255)
                                         ttatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtg                                                >  2:3686199/1‑93 (MQ=255)
                                          tatttatATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATtga                                               >  1:5193687/1‑93 (MQ=255)
                                              tataTTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGaa                                           >  2:1144459/1‑93 (MQ=255)
                                               atatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAt                                          >  2:6064802/1‑93 (MQ=255)
                                                tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg                                         >  1:4005743/1‑93 (MQ=255)
                                                tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg                                         <  2:1500368/93‑1 (MQ=255)
                                                tatTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATg                                         >  1:3496546/1‑93 (MQ=255)
                                                 atTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgc                                        >  2:2014828/1‑93 (MQ=255)
                                                   tttATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATgcgc                                      >  2:5820511/1‑93 (MQ=255)
                                                     tATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTTTGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGa                                    >  1:5020330/1‑93 (MQ=255)
                                                      aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                   >  1:1294973/1‑93 (MQ=255)
                                                      aTTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAc                                   >  1:3938257/1‑93 (MQ=255)
                                                         gTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg                                >  2:2253324/1‑93 (MQ=255)
                                                         gTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTg                                >  1:4902563/1‑93 (MQ=255)
                                                          tttATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTgg                               >  2:6361028/1‑93 (MQ=255)
                                                            tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt                             <  1:6672959/93‑1 (MQ=255)
                                                            tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt                             >  2:6473006/1‑93 (MQ=255)
                                                            tATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAt                             <  2:4504819/93‑1 (MQ=255)
                                                             aTTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGAtt                            >  2:557901/1‑93 (MQ=255)
                                                              tttCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTg                           >  2:3754687/1‑93 (MQ=255)
                                                                 taTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc                        <  1:1877545/92‑1 (MQ=255)
                                                                 cATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc                        >  1:1058175/1‑93 (MQ=255)
                                                                 cATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGccc                        <  2:2494831/93‑1 (MQ=255)
                                                                  aTGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCa                       >  2:260958/1‑93 (MQ=255)
                                                                   tGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAg                      <  1:162377/93‑1 (MQ=255)
                                                                    gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                     <  1:3903356/93‑1 (MQ=255)
                                                                    gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                     >  2:3873014/1‑93 (MQ=255)
                                                                    gCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGa                     <  2:3293861/93‑1 (MQ=255)
                                                                      aGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAcc                   >  1:6556786/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  2:1573646/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  1:867285/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  2:4849268/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  1:2182230/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  1:3048468/1‑93 (MQ=255)
                                                                       gCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCg                  >  1:5535144/1‑93 (MQ=255)
                                                                          aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg               >  2:4345616/1‑93 (MQ=255)
                                                                          aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg               >  2:4646201/1‑93 (MQ=255)
                                                                          aTTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTg               >  2:5288095/1‑93 (MQ=255)
                                                                           ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa              >  1:1388365/1‑93 (MQ=255)
                                                                           ttACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGa              >  2:6397346/1‑93 (MQ=255)
                                                                            tACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAc             <  1:459389/93‑1 (MQ=255)
                                                                              cacGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAg           >  1:6259071/1‑93 (MQ=255)
                                                                               acGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt          >  2:1460310/1‑93 (MQ=255)
                                                                               acGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGt          >  1:3371488/1‑93 (MQ=255)
                                                                                 gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa        >  2:764693/1‑93 (MQ=255)
                                                                                 gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa        >  1:5801597/1‑93 (MQ=255)
                                                                                 gATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGa        >  2:4230289/1‑93 (MQ=255)
                                                                                     aaCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc    <  1:380754/93‑1 (MQ=255)
                                                                                     aaCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGc    >  2:4538692/1‑93 (MQ=255)
                                                                                      aCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt   >  1:5733321/1‑93 (MQ=255)
                                                                                      aCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt   >  1:2586361/1‑93 (MQ=255)
                                                                                      aCGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCt   >  2:5187950/1‑93 (MQ=255)
                                                                                       cGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTg  <  1:1585234/93‑1 (MQ=255)
                                                                                          |                                                                                         
GATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTG  >  NC_000913/1768557‑1768736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  NC_000913/1768554‑1768740
                                                                                             |                                                                                             
CAAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCC                                                                                               <  SRR4302536.285727/94‑1 (MQ=60)
  AGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTA                                                                                             >  SRR4302536.5559244/1‑94 (MQ=60)
  AGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTA                                                                                             >  SRR4302536.1722271/1‑94 (MQ=60)
  AGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTA                                                                                             >  SRR4302536.6893340/1‑94 (MQ=60)
  AGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTA                                                                                             >  SRR4302536.5138179/1‑94 (MQ=60)
    ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           <  SRR4302536.1318763/94‑1 (MQ=60)
    ATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATG                                                                                           >  SRR4302536.2802086/1‑94 (MQ=60)
     TACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGA                                                                                          <  SRR4302536.4855478/94‑1 (MQ=60)
     TACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGA                                                                                          >  SRR4302536.2903135/1‑94 (MQ=60)
      ACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAA                                                                                         >  SRR4302536.4302431/1‑94 (MQ=60)
       CTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAAC                                                                                        >  SRR4302536.2059524/1‑94 (MQ=60)
         GTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTC                                                                                      >  SRR4302536.155432/1‑94 (MQ=60)
          TGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCC                                                                                     >  SRR4302536.5058072/1‑94 (MQ=60)
           GACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCA                                                                                    >  SRR4302536.2107073/1‑94 (MQ=60)
           GACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCA                                                                                    <  SRR4302536.3395049/94‑1 (MQ=60)
            ACTCTCTTCAAGCCGTCAGCAGTCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAG                                                                                   <  SRR4302536.6072770/94‑1 (MQ=60)
              TCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGC                                                                                 >  SRR4302536.2855658/1‑94 (MQ=60)
                 CTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACT                                                                              >  SRR4302536.854473/1‑94 (MQ=60)
                 CTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACT                                                                              <  SRR4302536.6060344/94‑1 (MQ=60)
                 CTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACT                                                                              <  SRR4302536.5482088/94‑1 (MQ=60)
                  TTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTC                                                                             >  SRR4302536.4480460/1‑94 (MQ=60)
                    CAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCG                                                                           <  SRR4302536.3728203/94‑1 (MQ=60)
                    CAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCG                                                                           <  SRR4302536.5267100/94‑1 (MQ=60)
                     AAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGC                                                                          >  SRR4302536.603022/1‑94 (MQ=60)
                     AAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGC                                                                          >  SRR4302536.6275002/1‑94 (MQ=60)
                       GCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCA                                                                        >  SRR4302536.6294455/1‑94 (MQ=60)
                       GCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCA                                                                        <  SRR4302536.6303462/94‑1 (MQ=60)
                       GCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCA                                                                        <  SRR4302536.3303370/94‑1 (MQ=60)
                       GCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCA                                                                        <  SRR4302536.1378133/94‑1 (MQ=60)
                        CCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCAT                                                                       <  SRR4302536.1678517/94‑1 (MQ=60)
                         CGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATA                                                                      >  SRR4302536.5608314/1‑94 (MQ=60)
                         CGTCAGCAGGCTGATAATATTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATA                                                                      >  SRR4302536.665823/1‑94 (MQ=60)
                          GTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATAT                                                                     >  SRR4302536.3363371/1‑94 (MQ=60)
                            CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   >  SRR4302536.2442612/1‑94 (MQ=60)
                            CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   >  SRR4302536.2552610/1‑94 (MQ=60)
                            CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   >  SRR4302536.5053857/1‑94 (MQ=60)
                            CAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTT                                                                   >  SRR4302536.4869555/1‑94 (MQ=60)
                             AGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTGCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACGCCGCCATATTTT                                                                  <  SRR4302536.6611532/94‑1 (MQ=60)
                              GCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTT                                                                 <  SRR4302536.2648340/94‑1 (MQ=60)
                               CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTG                                                                >  SRR4302536.5819079/1‑94 (MQ=60)
                               CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTG                                                                <  SRR4302536.982665/94‑1 (MQ=60)
                               CAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTG                                                                <  SRR4302536.6196788/94‑1 (MQ=60)
                                AGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGC                                                               >  SRR4302536.1386307/1‑94 (MQ=60)
                                 GGCTGATAATGTTAATTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCC                                                              >  SRR4302536.5787865/1‑94 (MQ=60)
                                  GCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCA                                                             <  SRR4302536.4837264/94‑1 (MQ=60)
                                   CTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCAT                                                            >  SRR4302536.3945413/1‑94 (MQ=60)
                                    TGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATG                                                           <  SRR4302536.4139349/94‑1 (MQ=60)
                                      ATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAC                                                         >  SRR4302536.4175366/1‑94 (MQ=60)
                                      ATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAC                                                         <  SRR4302536.1816302/94‑1 (MQ=60)
                                      ATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGAC                                                         >  SRR4302536.4175365/1‑94 (MQ=60)
                                       TAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACT                                                        <  SRR4302536.6140313/94‑1 (MQ=60)
                                       TAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACT                                                        >  SRR4302536.6819621/1‑94 (MQ=60)
                                        AATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTA                                                       >  SRR4302536.2232537/1‑94 (MQ=60)
                                        AATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTA                                                       >  SRR4302536.5153990/1‑94 (MQ=60)
                                         ATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTAT                                                      >  SRR4302536.6475722/1‑94 (MQ=60)
                                          TGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATT                                                     >  SRR4302536.1414900/1‑94 (MQ=60)
                                           GTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTG                                                    <  SRR4302536.6182355/94‑1 (MQ=60)
                                           GTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTG                                                    >  SRR4302536.2296826/1‑94 (MQ=60)
                                           GTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTG                                                    <  SRR4302536.1577721/94‑1 (MQ=60)
                                           GTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTG                                                    <  SRR4302536.155179/94‑1 (MQ=60)
                                            TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302536.5348550/1‑94 (MQ=60)
                                            TTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGA                                                   >  SRR4302536.365695/1‑94 (MQ=60)
                                             TATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGAT                                                  <  SRR4302536.5696168/94‑1 (MQ=60)
                                              ATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATG                                                 <  SRR4302536.1432319/94‑1 (MQ=60)
                                              ATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATG                                                 >  SRR4302536.5924766/1‑94 (MQ=60)
                                               TTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGA                                                >  SRR4302536.6509139/1‑94 (MQ=60)
                                                TTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAA                                               <  SRR4302536.291567/94‑1 (MQ=60)
                                                 TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              <  SRR4302536.408718/94‑1 (MQ=60)
                                                 TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              >  SRR4302536.3596814/1‑94 (MQ=60)
                                                 TATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAAT                                              <  SRR4302536.477793/94‑1 (MQ=60)
                                                  ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             <  SRR4302536.97322/94‑1 (MQ=60)
                                                  ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             >  SRR4302536.4124610/1‑94 (MQ=60)
                                                  ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             >  SRR4302536.3540558/1‑94 (MQ=60)
                                                  ATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATG                                             >  SRR4302536.3600880/1‑94 (MQ=60)
                                                   TATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGC                                            <  SRR4302536.4030996/94‑1 (MQ=60)
                                                      TTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCG                                         <  SRR4302536.3933830/94‑1 (MQ=60)
                                                       TTATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTTTGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGA                                        >  SRR4302536.5170814/1‑94 (MQ=60)
                                                        TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       >  SRR4302536.1980685/1‑94 (MQ=60)
                                                        TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       >  SRR4302536.1333685/1‑94 (MQ=60)
                                                        TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       >  SRR4302536.416435/1‑94 (MQ=60)
                                                        TATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGAC                                       >  SRR4302536.4055271/1‑94 (MQ=60)
                                                         ATTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACC                                      <  SRR4302536.2241448/94‑1 (MQ=60)
                                                          TTGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCT                                     <  SRR4302536.537428/94‑1 (MQ=60)
                                                           TGTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTG                                    >  SRR4302536.5049983/1‑94 (MQ=60)
                                                            GTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGG                                   <  SRR4302536.877810/94‑1 (MQ=60)
                                                            GTTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGG                                   >  SRR4302536.3837999/1‑94 (MQ=60)
                                                             TTTATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGA                                  >  SRR4302536.2224393/1‑94 (MQ=60)
                                                               TATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATT                                <  SRR4302536.6871223/94‑1 (MQ=60)
                                                                ATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTG                               >  SRR4302536.3963756/1‑94 (MQ=60)
                                                                ATTTCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTG                               <  SRR4302536.4959931/94‑1 (MQ=60)
                                                                   TCATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCC                            >  SRR4302536.1089818/1‑94 (MQ=60)
                                                                    TATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCA                           <  SRR4302536.1934041/94‑1 (MQ=60)
                                                                     ATGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAG                          >  SRR4302536.5789870/1‑94 (MQ=60)
                                                                      TGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCGCAGA                         >  SRR4302536.1862803/1‑94 (MQ=60)
                                                                      TGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGA                         <  SRR4302536.166591/94‑1 (MQ=60)
                                                                      TGCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGA                         >  SRR4302536.5889016/1‑94 (MQ=60)
                                                                       GCAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGAC                        <  SRR4302536.4019386/94‑1 (MQ=60)
                                                                        CAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACC                       >  SRR4302536.6752007/1‑94 (MQ=60)
                                                                        CAGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACC                       >  SRR4302536.458418/1‑94 (MQ=60)
                                                                         AGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCG                      >  SRR4302536.2248008/1‑94 (MQ=60)
                                                                         AGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCG                      >  SRR4302536.3138020/1‑94 (MQ=60)
                                                                         AGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCG                      >  SRR4302536.892802/1‑94 (MQ=60)
                                                                         AGCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCG                      >  SRR4302536.5700126/1‑94 (MQ=60)
                                                                          GCCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGG                     >  SRR4302536.2554689/1‑94 (MQ=60)
                                                                           CCATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGT                    <  SRR4302536.958007/94‑1 (MQ=60)
                                                                            CATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTG                   <  SRR4302536.6332111/94‑1 (MQ=60)
                                                                             ATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGA                  >  SRR4302536.2798718/1‑94 (MQ=60)
                                                                             ATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGA                  <  SRR4302536.2097121/94‑1 (MQ=60)
                                                                             ATTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGA                  >  SRR4302536.1429709/1‑94 (MQ=60)
                                                                              TTACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGAC                 <  SRR4302536.4370776/94‑1 (MQ=60)
                                                                               TACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACA                <  SRR4302536.471402/94‑1 (MQ=60)
                                                                                ACACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAG               >  SRR4302536.6445546/1‑94 (MQ=60)
                                                                                 CACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGT              >  SRR4302536.3471649/1‑94 (MQ=60)
                                                                                 CACGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGT              <  SRR4302536.6458259/94‑1 (MQ=60)
                                                                                   CGATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGA            >  SRR4302536.5974344/1‑94 (MQ=60)
                                                                                    GATGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCA                                                             >  SRR4302536.2215155/1‑44 (MQ=60)
                                                                                      TGAACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAG         <  SRR4302536.166322/94‑1 (MQ=60)
                                                                                        AACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT       >  SRR4302536.5903854/1‑94 (MQ=60)
                                                                                        AACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT       <  SRR4302536.390779/94‑1 (MQ=60)
                                                                                        AACGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCT       >  SRR4302536.2664063/1‑94 (MQ=60)
                                                                                          CGCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGG     <  SRR4302536.1631634/94‑1 (MQ=60)
                                                                                           GCCTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGC    <  SRR4302536.3362701/94‑1 (MQ=60)
                                                                                             CTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  SRR4302536.1068074/1‑94 (MQ=60)
                                                                                             |                                                                                             
CAAGATACTGTGACTCTCTTCAAGCCGTCAGCAGGCTGATAATGTTATTTATATTTTATTGTTTATTTCATGCAGCCATTACACGATGAACGCTTATGAACTCCAGGCACTCCGCCATATTTTTGCCATGACTATTGATGAATGCGCGACCTGGATTGCCCAGACCGGTGACAGTGAAAGCTGGCGT  >  NC_000913/1768554‑1768740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑