Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,772,080 G→A A90T (GCC→ACC)  ydiB → putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,772,0800GA99.5% 719.8 / ‑5.3 199A90T (GCC→ACC) ydiBputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (92/106);  total (93/106)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 212 total aligned reads displayed.

AAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGT  >  NC_000913/1771990‑1772169
                                                                                          |                                                                                         
 aaaTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCa                                                                                        <  2:2664463/93‑1 (MQ=255)
 aaaTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCa                                                                                        >  2:2954892/1‑93 (MQ=255)
 aaaTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCa                                                                                        >  2:1508304/1‑93 (MQ=255)
  aaTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCAt                                                                                       <  2:3775884/93‑1 (MQ=255)
   aTGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCAta                                                                                      >  2:3520038/1‑92 (MQ=255)
       gcgGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAaca                                                                                  <  2:1831152/93‑1 (MQ=255)
       gcgGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAaca                                                                                  <  1:1384713/93‑1 (MQ=255)
       gcgGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAaca                                                                                  <  1:81286/93‑1 (MQ=255)
        cgGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAacac                                                                                 <  2:5541671/93‑1 (MQ=255)
          gAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCa                                                                               >  1:581242/1‑93 (MQ=255)
          gAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCa                                                                               >  2:5847965/1‑93 (MQ=255)
           aaCTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCAt                                                                              <  2:3386502/93‑1 (MQ=255)
             cTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCg                                                                            <  1:6695691/93‑1 (MQ=255)
              tGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGt                                                                           >  2:5136968/1‑93 (MQ=255)
               ggTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGtt                                                                          <  1:4442134/93‑1 (MQ=255)
                  gtATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAat                                                                       <  2:2895475/93‑1 (MQ=255)
                    aTCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatga                                                                     <  1:2381723/93‑1 (MQ=255)
                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgat                                                                    >  1:5968003/1‑93 (MQ=255)
                     tCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgat                                                                    >  2:4645058/1‑93 (MQ=255)
                      cGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACAGCAGCGGCCAAACTGGTGGGGAACATCAACACCATCGTTAatgatg                                                                   >  1:1736919/1‑93 (MQ=255)
                       gATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatga                                                                  <  2:1644595/93‑1 (MQ=255)
                       gATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatga                                                                  <  1:1557762/93‑1 (MQ=255)
                       gATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatga                                                                  <  1:6654282/93‑1 (MQ=255)
                       gATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatga                                                                  <  2:874060/93‑1 (MQ=255)
                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatgat                                                                 <  2:2620028/93‑1 (MQ=255)
                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatgat                                                                 >  1:2738772/1‑93 (MQ=255)
                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatgat                                                                 <  1:2398381/93‑1 (MQ=255)
                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatgat                                                                 <  1:517389/93‑1 (MQ=255)
                        aTGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAatgatgat                                                                 >  2:3618141/1‑93 (MQ=255)
                          gCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATgg                                                               <  2:5659578/93‑1 (MQ=255)
                          gCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATgg                                                               >  2:835522/1‑93 (MQ=255)
                           ccGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGc                                                              <  2:495202/93‑1 (MQ=255)
                             gAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTa                                                            >  1:367041/1‑93 (MQ=255)
                               acaaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATc                                                          <  2:2266528/93‑1 (MQ=255)
                               acaaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATc                                                          <  2:6264250/93‑1 (MQ=255)
                               acaaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATc                                                          >  2:1554158/1‑93 (MQ=255)
                                 aaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTg                                                        >  2:468800/1‑93 (MQ=255)
                                 aaacaaCTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTg                                                        >  1:4162470/1‑93 (MQ=255)
                                  aacaacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATGGTTAATGATGATGGCTATTTGc                                                       >  1:4060097/1‑93 (MQ=255)
                                  aacaacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGc                                                       >  2:1922204/1‑93 (MQ=255)
                                   acaacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCg                                                      >  1:3225579/1‑93 (MQ=255)
                                    caacTGGCGTGTTAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGt                                                     <  1:4243183/93‑1 (MQ=255)
                                    caacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGt                                                     <  2:3306370/93‑1 (MQ=255)
                                    caacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGt                                                     <  1:1050093/93‑1 (MQ=255)
                                     aacTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTg                                                    <  1:5590301/93‑1 (MQ=255)
                                      acTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTgg                                                   >  2:5963193/1‑93 (MQ=255)
                                      acTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTgg                                                   >  1:3071477/1‑93 (MQ=255)
                                      acTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTgg                                                   >  1:5456942/1‑93 (MQ=255)
                                       cTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGc                                                  >  1:351160/1‑93 (MQ=255)
                                            gtgtGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATaa                                             >  1:6170645/1‑93 (MQ=255)
                                            gtgtGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATaa                                             >  2:3421526/1‑93 (MQ=255)
                                            gtgtGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATaa                                             <  1:2236729/93‑1 (MQ=255)
                                             tgtgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAac                                            >  1:1268945/1‑93 (MQ=255)
                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAacac                                          <  1:5740356/93‑1 (MQ=255)
                                               tgAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAacac                                          <  1:4353998/93‑1 (MQ=255)
                                                gAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACAcc                                         <  2:6487818/93‑1 (MQ=255)
                                                 aaTATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCg                                        >  1:2921002/1‑93 (MQ=255)
                                                  atatGTTGATGAATTAGCACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGa                                       >  1:3377866/1‑93 (MQ=255)
                                                  atatGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGa                                       <  2:4277377/93‑1 (MQ=255)
                                                     tGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACgg                                    <  2:4350018/93‑1 (MQ=255)
                                                       ttGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCa                                  >  2:3454647/1‑93 (MQ=255)
                                                        tgatgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCAc                                 <  2:4530056/93‑1 (MQ=255)
                                                         gatgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACg                                >  1:2615100/1‑93 (MQ=255)
                                                          atgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACgg                               <  2:2488396/93‑1 (MQ=255)
                                                          atgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACgg                               <  1:4654770/93‑1 (MQ=255)
                                                          atgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACgg                               <  1:3167464/93‑1 (MQ=255)
                                                           tgaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACggg                              <  1:6053158/93‑1 (MQ=255)
                                                            gaATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCAACGGCACGGGc                             <  2:682155/93‑1 (MQ=255)
                                                             aaTTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGcc                            <  1:918376/93‑1 (MQ=255)
                                                               ttAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCat                          >  2:663656/1‑93 (MQ=255)
                                                                 aaCACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCatat                        >  2:3358186/1‑93 (MQ=255)
                                                                 aaCACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCatat                        >  2:4044184/1‑93 (MQ=255)
                                                                  acacCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATAtt                       <  1:5423973/93‑1 (MQ=255)
                                                                   cacCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                      >  2:2098475/1‑93 (MQ=255)
                                                                   cacCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTc                      >  1:2357093/1‑93 (MQ=255)
                                                                      cAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTcgcg                   >  2:700047/1‑93 (MQ=255)
                                                                       aGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCgcgc                  >  2:5280761/1‑93 (MQ=255)
                                                                        gCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGcc                 >  1:4413609/1‑93 (MQ=255)
                                                                        gCTGCAAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAGGGCTATCTGCGTGTCTATAACACCGACGGCACGGGCCATATTCGCGcc                 <  1:3459005/93‑1 (MQ=255)
                                                                        acTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGcc                 <  2:1415854/92‑1 (MQ=255)
                                                                          tGCCAAGCTGGTGGGGACCATCAACCCCATCGTTAATGATGATGACTATCTGCGTGGCAATAACACCGACGGCCCGGGCCATATTCGCGCCAt               <  1:1575175/93‑1 (MQ=255)
                                                                          tGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCAt               <  1:1094911/93‑1 (MQ=255)
                                                                          tGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCAt               >  2:2514270/1‑93 (MQ=255)
                                                                          tGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCAt               >  2:1808598/1‑93 (MQ=255)
                                                                           cccAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCAtt              <  2:237830/92‑1 (MQ=255)
                                                                             cAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTaa            >  2:5418430/1‑93 (MQ=255)
                                                                              aaaCTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTaaa           <  2:5342089/93‑1 (MQ=255)
                                                                              aaaCTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTaaa           <  2:1089654/93‑1 (MQ=255)
                                                                               aaCTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAag          >  2:6152086/1‑93 (MQ=255)
                                                                                aCTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAaga         <  1:1139657/93‑1 (MQ=255)
                                                                                 cTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagag        <  1:4906128/93‑1 (MQ=255)
                                                                                  tggtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACCCCGACGGCACGGGCCATATTCGCGCCATTAAagaga       <  1:2676731/93‑1 (MQ=255)
                                                                                  tggtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagaga       <  1:5632843/93‑1 (MQ=255)
                                                                                  tggtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagaga       <  2:6073214/93‑1 (MQ=255)
                                                                                  tggtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagaga       <  1:4775532/93‑1 (MQ=255)
                                                                                   ggtggGGACCATCAACACCATCGTTAATGATGATGGCTAACTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAagagag      <  1:6425181/93‑1 (MQ=255)
                                                                                    gtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGc     <  1:4567025/93‑1 (MQ=255)
                                                                                    gtggGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGc     <  1:3336400/93‑1 (MQ=255)
                                                                                      ggggaCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCgg   >  1:1865408/1‑93 (MQ=255)
                                                                                       gggaCCATCAACGCCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGt  >  2:4080149/1‑93 (MQ=255)
                                                                                          |                                                                                         
AAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGT  >  NC_000913/1771990‑1772169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTG  >  NC_000913/1771987‑1772173
                                                                                             |                                                                                             
CTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGA                                                                                               >  SRR4302536.500436/1‑94 (MQ=60)
CTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGA                                                                                               <  SRR4302536.5756773/94‑1 (MQ=60)
    AAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGTGACCAT                                                                                           <  SRR4302536.4347160/94‑1 (MQ=60)
     AATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATC                                                                                          <  SRR4302536.2560694/94‑1 (MQ=60)
          GCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACAC                                                                                     <  SRR4302536.1425958/94‑1 (MQ=60)
          GCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACAC                                                                                     <  SRR4302536.83468/94‑1 (MQ=60)
            GGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCA                                                                                   >  SRR4302536.596001/1‑94 (MQ=60)
            GGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCA                                                                                   >  SRR4302536.3064094/1‑94 (MQ=60)
             GAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCAT                                                                                  <  SRR4302536.1394647/94‑1 (MQ=60)
              AACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATC                                                                                 <  SRR4302536.4260705/94‑1 (MQ=60)
                CTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGT                                                                               <  SRR4302536.6894548/94‑1 (MQ=60)
                CTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGT                                                                               >  SRR4302536.997439/1‑94 (MQ=60)
                CTGGTGTATAGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGT                                                                               >  SRR4302536.6231676/1‑94 (MQ=60)
                 TGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTT                                                                              <  SRR4302536.3885267/94‑1 (MQ=60)
                  GGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTA                                                                             <  SRR4302536.1099992/94‑1 (MQ=60)
                  GGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTA                                                                             <  SRR4302536.4575515/94‑1 (MQ=60)
                  GGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTA                                                                             >  SRR4302536.940446/1‑94 (MQ=60)
                    TGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAAT                                                                           <  SRR4302536.1621397/94‑1 (MQ=60)
                      TATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGA                                                                         >  SRR4302536.6668773/1‑94 (MQ=60)
                       ATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGAT                                                                        >  SRR4302536.6145866/1‑94 (MQ=60)
                       ATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGAT                                                                        <  SRR4302536.2453873/94‑1 (MQ=60)
                       ATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGAT                                                                        >  SRR4302536.2435420/1‑94 (MQ=60)
                        TCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACAGCAGCGGCCAAACTGGTGGGGAACATCAACACCATCGTTAATGATG                                                                       >  SRR4302536.1787018/1‑94 (MQ=60)
                          GATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAT                                                                     <  SRR4302536.3276666/94‑1 (MQ=60)
                          GATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAT                                                                     <  SRR4302536.1603515/94‑1 (MQ=60)
                          GATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAT                                                                     <  SRR4302536.4736015/94‑1 (MQ=60)
                          GATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAT                                                                     >  SRR4302536.2820375/1‑94 (MQ=60)
                          GATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAT                                                                     <  SRR4302536.6852063/94‑1 (MQ=60)
                           ATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATG                                                                    <  SRR4302536.530657/94‑1 (MQ=60)
                           ATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATG                                                                    <  SRR4302536.2470979/94‑1 (MQ=60)
                           ATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATG                                                                    >  SRR4302536.4645301/1‑94 (MQ=60)
                              CCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCT                                                                 <  SRR4302536.5361897/94‑1 (MQ=60)
                               CGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTA                                                                <  SRR4302536.1619140/94‑1 (MQ=60)
                               CGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTA                                                                <  SRR4302536.3456468/94‑1 (MQ=60)
                               CGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTA                                                                >  SRR4302536.376742/1‑94 (MQ=60)
                                   CAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTG                                                            <  SRR4302536.6440073/94‑1 (MQ=60)
                                   CAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTG                                                            >  SRR4302536.4286120/1‑94 (MQ=60)
                                    AAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATGGTTAATGATGATGGCTATTTGC                                                           >  SRR4302536.4180442/1‑94 (MQ=60)
                                    AAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGC                                                           <  SRR4302536.2803563/94‑1 (MQ=60)
                                     AACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCG                                                          >  SRR4302536.3320144/1‑94 (MQ=60)
                                       CAACTGGCGTGTTAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTG                                                        <  SRR4302536.4369545/94‑1 (MQ=60)
                                       CAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTG                                                        <  SRR4302536.1081415/94‑1 (MQ=60)
                                        AACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGG                                                       >  SRR4302536.3161459/1‑94 (MQ=60)
                                        AACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGG                                                       >  SRR4302536.3611930/1‑94 (MQ=60)
                                        AACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGG                                                       >  SRR4302536.5620111/1‑94 (MQ=60)
                                        AACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGG                                                       <  SRR4302536.5756555/94‑1 (MQ=60)
                                         ACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGC                                                      >  SRR4302536.360466/1‑94 (MQ=60)
                                           TGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTA                                                    <  SRR4302536.5855243/94‑1 (MQ=60)
                                              CGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAA                                                 >  SRR4302536.6354435/1‑94 (MQ=60)
                                               GTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAAC                                                >  SRR4302536.1306960/1‑94 (MQ=60)
                                               GTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAAC                                                <  SRR4302536.2304394/94‑1 (MQ=60)
                                                TGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACA                                               >  SRR4302536.4039739/1‑94 (MQ=60)
                                                  TGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACC                                             <  SRR4302536.4484244/94‑1 (MQ=60)
                                                  TGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACC                                             <  SRR4302536.5911123/94‑1 (MQ=60)
                                                   GAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCG                                            >  SRR4302536.3007209/1‑94 (MQ=60)
                                                   GAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCG                                            <  SRR4302536.5671461/94‑1 (MQ=60)
                                                    AATATGTTGATGAATTAGCACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGA                                           >  SRR4302536.3478242/1‑94 (MQ=60)
                                                    AATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGA                                           >  SRR4302536.6246914/1‑94 (MQ=60)
                                                     ATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGAC                                          <  SRR4302536.1494459/94‑1 (MQ=60)
                                                     ATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGAC                                          >  SRR4302536.2348772/1‑94 (MQ=60)
                                                     ATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGAC                                          >  SRR4302536.5272305/1‑94 (MQ=60)
                                                     ATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGAC                                          <  SRR4302536.3492999/94‑1 (MQ=60)
                                                     ATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGAC                                          >  SRR4302536.724401/1‑94 (MQ=60)
                                                      TATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACG                                         <  SRR4302536.6000360/94‑1 (MQ=60)
                                                       ATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGG                                        <  SRR4302536.4946857/94‑1 (MQ=60)
                                                       ATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGG                                        >  SRR4302536.2747397/1‑94 (MQ=60)
                                                        TGTTGATGAATTAACTCCAGCTGCAAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGC                                       <  SRR4302536.6860056/94‑1 (MQ=60)
                                                           TGATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACG                                    >  SRR4302536.2693560/1‑94 (MQ=60)
                                                            GATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGG                                   >  SRR4302536.3428524/1‑94 (MQ=60)
                                                             ATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGG                                  <  SRR4302536.3260261/94‑1 (MQ=60)
                                                             ATGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGG                                  <  SRR4302536.4794716/94‑1 (MQ=60)
                                                              TGAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGC                                 <  SRR4302536.6233730/94‑1 (MQ=60)
                                                               GAATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCC                                <  SRR4302536.1792863/94‑1 (MQ=60)
                                                                AATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCA                               <  SRR4302536.945444/94‑1 (MQ=60)
                                                                 ATTAACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCAT                              >  SRR4302536.1254171/1‑94 (MQ=60)
                                                                 ATTAACACCAGCTGCCAAACGGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCAT                              <  SRR4302536.5932734/94‑1 (MQ=60)
                                                                     ACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTC                          >  SRR4302536.2297623/1‑94 (MQ=60)
                                                                     ACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTC                          <  SRR4302536.5586120/94‑1 (MQ=60)
                                                                     ACACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTC                          >  SRR4302536.2428535/1‑94 (MQ=60)
                                                                      CACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCG                         <  SRR4302536.3228356/94‑1 (MQ=60)
                                                                      CACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCG                         >  SRR4302536.4891847/1‑94 (MQ=60)
                                                                       ACCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGC                        >  SRR4302536.2022720/1‑94 (MQ=60)
                                                                        CCAGCTGCCAAACTGTTGGGGACCATCAGCACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCG                       <  SRR4302536.5796239/94‑1 (MQ=60)
                                                                        CCAGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCG                       >  SRR4302536.455289/1‑94 (MQ=60)
                                                                          AGCTGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCC                     >  SRR4302536.4545698/1‑94 (MQ=60)
                                                                           GCTGCAAAACTGGTGGGGACCATCAACACCATCGTTAATGATGAGGGCTATCTGCGTGTCTATAACACCGACGGCACGGGCCATATTCGCGCCA                    <  SRR4302536.3562056/94‑1 (MQ=60)
                                                                             TGCCAAGCTGGTGGGGACCATCAACCCCATCGTTAATGATGATGACTATCTGCGTGGCAATAACACCGACGGCCCGGGCCATATTCGCGCCATT                  <  SRR4302536.1621338/94‑1 (MQ=60)
                                                                             TGCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATT                  <  SRR4302536.1127759/94‑1 (MQ=60)
                                                                              GCCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTA                 <  SRR4302536.4476221/94‑1 (MQ=60)
                                                                               CCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAA                <  SRR4302536.5009401/94‑1 (MQ=60)
                                                                               CCAAACTGGTGGGGACCATCAACACCATCGTTAATGATGATCGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATGTTCACGCCATTAA                >  SRR4302536.1963130/1‑94 (MQ=60)
                                                                                 AACCTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAG              <  SRR4302536.6095352/94‑1 (MQ=60)
                                                                                 AAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAG              >  SRR4302536.1137261/1‑94 (MQ=60)
                                                                                 AAACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAG              >  SRR4302536.2987683/1‑94 (MQ=60)
                                                                                 AAACTGGCGGGTACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAACG              >  SRR4302536.746939/1‑94 (MQ=60)
                                                                                  AACTGGGTGGGCCCATCAACACCATCGTTAATGCTGAGGGCTATCTACGAGGCCATAACACCGACGGCAGGCGCCATATTCCTGCCATTAGAGA             >  SRR4302536.6102856/1‑94 (MQ=23)
                                                                                   ACTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAG            <  SRR4302536.1173919/94‑1 (MQ=60)
                                                                                    CTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGA           <  SRR4302536.5599383/94‑1 (MQ=60)
                                                                                    CTGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGA           <  SRR4302536.5053623/94‑1 (MQ=60)
                                                                                     TGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACCCCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAG          <  SRR4302536.2756732/94‑1 (MQ=60)
                                                                                     TGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAG          <  SRR4302536.4919323/94‑1 (MQ=60)
                                                                                     TGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAG          <  SRR4302536.5800170/94‑1 (MQ=60)
                                                                                     TGGTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAG          <  SRR4302536.3654487/94‑1 (MQ=60)
                                                                                      GGTGGGGACCATCAACACCATCGTTAATGATGATGGCTAACTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGC         <  SRR4302536.6617226/94‑1 (MQ=60)
                                                                                       GTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCG        <  SRR4302536.4704074/94‑1 (MQ=60)
                                                                                       GTGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCG        <  SRR4302536.3435207/94‑1 (MQ=60)
                                                                                        TGGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGG       >  SRR4302536.1921568/1‑94 (MQ=60)
                                                                                         GGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGT      <  SRR4302536.3139425/94‑1 (MQ=60)
                                                                                         GGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGT      >  SRR4302536.245302/1‑94 (MQ=60)
                                                                                         GGGGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGT      <  SRR4302536.4516846/94‑1 (MQ=60)
                                                                                           GGACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTT    <  SRR4302536.2225950/94‑1 (MQ=60)
                                                                                            GACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGCCGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTT   <  SRR4302536.310675/94‑1 (MQ=60)
                                                                                            GACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTT   <  SRR4302536.6468610/94‑1 (MQ=60)
                                                                                            GACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTT   >  SRR4302536.2571950/1‑94 (MQ=60)
                                                                                            GACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTT   >  SRR4302536.4150933/1‑94 (MQ=60)
                                                                                             ACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTG  <  SRR4302536.3765326/94‑1 (MQ=60)
                                                                                             ACCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTG  <  SRR4302536.16660/94‑1 (MQ=60)
                                                                                             |                                                                                             
CTCAAAATGCGCGGAACTGGTGTATCGATGCCGAACAAACAACTGGCGTGTGAATATGTTGATGAATTAACACCAGCTGCCAAACTGGTGGGGGCCATCAACACCATCGTTAATGATGATGGCTATCTGCGTGGCTATAACACCGACGGCACGGGCCATATTCGCGCCATTAAAGAGAGCGGTTTTG  >  NC_000913/1771987‑1772173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑