Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,097	(A)_7→6	intergenic (+14/+42)	purT → / ← eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,091	0	A	.	99.4%	780.7 / ‑3.2	170	intergenic (+8/+48)	purT/eda	phosphoribosylglycinamide formyltransferase 2/2‑keto‑3‑deoxygluconate 6‑phosphate aldolase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (77/92); minor base T (1/0); total (78/92)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

TGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG  >  NC_000913/1930001‑1930179

 ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaa                                                                                       <  1:5554795/93‑1 (MQ=255)

 ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaa                                                                                       >  1:1981540/1‑93 (MQ=255)

 ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaa                                                                                       >  1:6031253/1‑93 (MQ=255)

  gCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaa                                                                                      >  1:668248/1‑93 (MQ=255)

      tGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgc                                                                                  >  2:6524748/1‑90 (MQ=255)

      tGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgc                                                                                  <  2:2239028/93‑4 (MQ=255)

       ggCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCAGGCaaaaaacgct                                                                                 >  2:1049117/1‑89 (MQ=255)

       ggCTACTGCAGAGAGTGTTGTTAACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCaaaaaacgct                                                                                 >  2:4351374/1‑89 (MQ=255)

         cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                              >  2:1765384/1‑93 (MQ=255)

         cTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAc                                                                              <  1:1652309/93‑1 (MQ=255)

          tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACa                                                                             >  2:1183633/1‑93 (MQ=255)

           aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaa                                                                            >  1:1816239/1‑93 (MQ=255)

              gCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACaaaaa                                                                         <  2:2753321/93‑1 (MQ=255)

               cAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAt                                                                        <  1:2810533/93‑1 (MQ=255)

               cAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAt                                                                        <  1:3941702/93‑1 (MQ=255)

               cAGAGAGGGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAt                                                                        <  2:3305255/93‑1 (MQ=255)

               cAGAGAGAGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACACGGC‑AAAAAACGCTACAACAAt                                                                        >  2:3462439/1‑93 (MQ=255)

                agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATg                                                                       >  2:2049341/1‑93 (MQ=255)

                agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATg                                                                       <  2:3096753/93‑1 (MQ=255)

                agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATg                                                                       >  1:3748200/1‑93 (MQ=255)

                 gggagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                      <  2:6250493/91‑1 (MQ=255)

                 gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                      >  1:5934680/1‑93 (MQ=255)

                 gagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGc                                                                      <  2:898260/93‑1 (MQ=255)

                  agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     <  1:4277848/93‑1 (MQ=255)

                  agagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGcc                                                                     <  2:3204377/93‑1 (MQ=255)

                   gagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGccc                                                                    >  2:4853975/1‑93 (MQ=255)

                     gtgtTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGa                                                                  >  1:643646/1‑93 (MQ=255)

                      tgttgtTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAt                                                                 <  1:3860966/93‑1 (MQ=255)

                       gttgttGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATc                                                                <  1:2045388/93‑1 (MQ=255)

                       gttgttGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATc                                                                <  2:345349/93‑1 (MQ=255)

                        ttgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATcc                                                               <  1:6132145/93‑1 (MQ=255)

                         tgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCt                                                              <  2:730776/93‑1 (MQ=255)

                         tgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCt                                                              <  2:5681385/93‑1 (MQ=255)

                          gttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTc                                                             >  1:4362715/1‑93 (MQ=255)

                           ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCg                                                            <  2:3879195/93‑1 (MQ=255)

                           ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCg                                                            <  1:470173/93‑1 (MQ=255)

                            tgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGa                                                           <  1:2783964/93‑1 (MQ=255)

                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                          <  1:3554847/93‑1 (MQ=255)

                             gACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                          <  2:1358124/93‑1 (MQ=255)

                              aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                         >  2:4868836/1‑93 (MQ=255)

                              aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATc                                                         <  2:579493/93‑1 (MQ=255)

                               cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCg                                                        <  1:2197868/93‑1 (MQ=255)

                                gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                       <  1:5040890/93‑1 (MQ=255)

                                gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                       <  1:780445/93‑1 (MQ=255)

                                   aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCa                                                    >  2:5247114/1‑93 (MQ=255)

                                     tGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtt                                                  >  2:4145472/1‑93 (MQ=255)

                                        aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAttttt                                               >  2:209547/1‑92 (MQ=255)

                                        aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTg                                               >  2:3612083/1‑93 (MQ=255)

                                         cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa                                              <  1:5166081/93‑1 (MQ=255)

                                          gcgagAAGCCCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAc                                             <  1:2734297/93‑1 (MQ=255)

                                            gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                           >  2:6378344/1‑93 (MQ=255)

                                            gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtt                                           >  2:3779206/1‑93 (MQ=255)

                                             cgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACttt                                          <  1:4081388/93‑1 (MQ=255)

                                              gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                         <  1:1081729/93‑1 (MQ=255)

                                              gAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACACGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                         >  2:6030496/1‑93 (MQ=255)

                                               aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTa                                        >  2:325827/1‑93 (MQ=255)

                                                aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGGATTTTTACtttttc                                       >  2:3411048/1‑91 (MQ=255)

                                                aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                       <  1:2273596/93‑1 (MQ=255)

                                                aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                       >  2:214367/1‑93 (MQ=255)

                                                aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTAc                                       <  1:2118667/93‑1 (MQ=255)

                                                 gCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                      <  1:2149889/93‑1 (MQ=255)

                                                 gCACGCCGCCGGACAGGTAAAAGTACAGGGTAAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACa                                      <  2:1244597/93‑1 (MQ=255)

                                                  cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAg                                     >  2:2249929/1‑93 (MQ=255)

                                                   aCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCCCGATCGGGCGTTTTGACCTTTACAGc                                    >  1:4761673/1‑93 (MQ=255)

                                                    cgccgcCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCt                                   <  1:1076053/93‑1 (MQ=255)

                                                     gccgccGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTGTACAGCtt                                  <  1:6524748/93‑1 (MQ=255)

                                                      ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTa                                 <  1:3103044/93‑1 (MQ=255)

                                                       cgccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAg                                <  1:2919907/93‑1 (MQ=255)

                                                        gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGGCCGATCCTCGATCGGGCATTTTGAATTTTACAGCTTAgc                               >  1:2413944/1‑93 (MQ=255)

                                                        gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                               >  2:5510828/1‑93 (MQ=255)

                                                        gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgc                               <  2:5214427/93‑1 (MQ=255)

                                                        gccgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCGGGCCTTTTTTCTTTTttagcttagc                               >  1:4679074/1‑93 (MQ=255)

                                                          cgGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                             >  1:3355865/1‑93 (MQ=255)

                                                           ggACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                            >  1:433398/1‑93 (MQ=255)

                                                           ggACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                            <  2:4765072/93‑1 (MQ=255)

                                                            gACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCt                           <  1:4521734/93‑1 (MQ=255)

                                                              cAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACTCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTc                         <  1:1995723/93‑1 (MQ=255)

                                                              cAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCTGGCATTTTGACTTTTACAGCTTAGCGCCTTc                         >  1:2574760/1‑93 (MQ=255)

                                                               aGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCt                        <  2:1837191/93‑1 (MQ=255)

                                                                  tAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTATAGCTTATCGCCTTCTTCa                     >  1:1149983/1‑93 (MQ=255)

                                                                   aaaaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                    >  2:429840/1‑93 (MQ=255)

                                                                    aaaGTACAGGGTTAAACCCCGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCCGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGc                   >  2:6222082/1‑93 (MQ=255)

                                                                     aaatACAGGGTTAAA‑CCGGGAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGTCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCt                  <  2:4550793/90‑1 (MQ=255)

                                                                     aaGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCt                  <  1:4001479/93‑1 (MQ=255)

                                                                      aGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                 >  1:4919323/1‑93 (MQ=255)

                                                                      aGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                 >  2:2972317/1‑93 (MQ=255)

                                                                      aGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCtt                 >  1:630055/1‑93 (MQ=255)

                                                                       gTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                >  1:3284493/1‑93 (MQ=255)

                                                                       gTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                <  1:977393/93‑1 (MQ=255)

                                                                       gTACAGGA‑TAAACCCCGAAAAAAACCGCTAAAAAAATGCACGTTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                <  2:3575104/93‑1 (MQ=255)

                                                                       gTAAAGGGTAAAACCCGGC‑AAAAAACGCTAAAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTc                <  1:1284612/93‑1 (MQ=255)

                                                                           aGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc            <  1:3174604/93‑1 (MQ=255)

                                                                           aGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgc            >  2:6665496/1‑93 (MQ=255)

                                                                            gggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAcgcg           >  1:1511115/1‑93 (MQ=255)

                                                                             ggTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACgcgc          >  2:2263507/1‑93 (MQ=255)

                                                                              gTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGcc         >  1:5596875/1‑93 (MQ=255)

                                                                               ttAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTTTAGCTTAGCGCCTTCTACCGCTTCACGCGccc        >  2:2403500/1‑92 (MQ=255)

                                                                                    cccGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTa   <  2:4670543/93‑1 (MQ=255)

                                                                                     ccGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAg  <  2:29003/93‑1 (MQ=255)

                                                                                     ccGGC‑AAAAAACACTACAAAAATGCCAGATCCTCGATCTGGCATTTTGACTTTTACAGCTTCGCGCCTTCTACAGCTTCACGCGCCAGCTTAg  <  2:2620398/93‑1 (MQ=255)

TGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG  >  NC_000913/1930001‑1930179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  >  NC_000913/1930004‑1930189

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAC                                                                                              >  SRR4302536.4971033/1‑94 (MQ=60)

  CTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGC                                                                                           >  SRR4302536.3833617/1‑94 (MQ=60)

   TGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACACGGC‑AAAAAACGCT                                                                                          >  SRR4302536.2210100/1‑94 (MQ=60)

     GCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTAC                                                                                        >  SRR4302536.3948237/1‑94 (MQ=60)

      CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       >  SRR4302536.202537/1‑94 (MQ=60)

      CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACA                                                                                       <  SRR4302536.1700328/94‑1 (MQ=60)

      CTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGGTAAAAACGGCAAAAAAAaaaaaa                                                                                        >  SRR4302536.4848143/1‑88 (MQ=60)

       TACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAA                                                                                      >  SRR4302536.2243488/1‑94 (MQ=60)

       TACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAA                                                                                      >  SRR4302536.1871040/1‑94 (MQ=60)

          TGCAGACGGTGTTGTTGACGCCATTTAACGTGCGAAGCACTCCCCCGTACAGCGAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAA                                                                                   >  SRR4302536.814385/1‑94 (MQ=41)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302536.1152634/94‑1 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302536.2338851/94‑1 (MQ=60)

           GCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAAT                                                                                  <  SRR4302536.4422194/94‑1 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 >  SRR4302536.3860238/1‑94 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 <  SRR4302536.2893846/94‑1 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 >  SRR4302536.2044044/1‑94 (MQ=60)

            CAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATG                                                                                 <  SRR4302536.4058796/94‑1 (MQ=60)

             AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                <  SRR4302536.510286/94‑1 (MQ=60)

             AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                <  SRR4302536.5840907/94‑1 (MQ=60)

             AGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGC                                                                                >  SRR4302536.6111458/1‑94 (MQ=60)

              GAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCC                                                                               >  SRR4302536.1401528/1‑94 (MQ=60)

               AGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCC                                                                              <  SRR4302536.4405392/94‑1 (MQ=60)

               AGAGTGTGGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCC                                                                              <  SRR4302536.1190154/94‑1 (MQ=60)

                 ATTGTTTTTGACGCCATTGACCGCGCGAAGCACGCCGCCGGACA‑GTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGAT                                                                           <  SRR4302536.1868794/94‑1 (MQ=60)

                 AGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGA                                                                            >  SRR4302536.662614/1‑94 (MQ=60)

                 AGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGG‑TAAAAAACGCTACAAAAATGCCCGA                                                                            >  SRR4302536.5279648/1‑94 (MQ=60)

                   TGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATC                                                                          <  SRR4302536.3975848/94‑1 (MQ=60)

                    GTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCC                                                                         <  SRR4302536.2106969/94‑1 (MQ=60)

                     TTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCT                                                                        <  SRR4302536.6314897/94‑1 (MQ=60)

                     TTGTTGACGCCATGGAACTCGCGAGGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGggcct                                                                        <  SRR4302536.4852199/94‑6 (MQ=60)

                      TGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTC                                                                       >  SRR4302536.4493107/1‑94 (MQ=60)

                      TGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTC                                                                       >  SRR4302536.4581646/1‑94 (MQ=60)

                      TGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTC                                                                       <  SRR4302536.6252478/94‑1 (MQ=60)

                        TTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGA                                                                     <  SRR4302536.482448/94‑1 (MQ=60)

                         TGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGAT                                                                    <  SRR4302536.2866650/94‑1 (MQ=60)

                          GACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATC                                                                   <  SRR4302536.3661139/94‑1 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                 <  SRR4302536.2264218/94‑1 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                 <  SRR4302536.3507625/94‑1 (MQ=60)

                            CGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGG                                                                 >  SRR4302536.2081850/1‑94 (MQ=60)

                             GCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGG                                                                <  SRR4302536.5191971/94‑1 (MQ=60)

                             GCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGG                                                                <  SRR4302536.6182453/94‑1 (MQ=60)

                             GCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGG                                                                >  SRR4302536.867244/1‑94 (MQ=60)

                             GCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGG                                                                <  SRR4302536.803309/94‑1 (MQ=60)

                                 tcgagCGCGCGAACCACGCCGCCGGACAGGAAAAAGTACAGGGTAAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATT                                                            <  SRR4302536.2667956/89‑1 (MQ=60)

                                   GAACGTGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTT                                                          <  SRR4302536.6701969/94‑1 (MQ=60)

                                      CGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGAC                                                       <  SRR4302536.5320220/94‑1 (MQ=60)

                                       GCGAGAAGCCCGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACT                                                      <  SRR4302536.2815790/94‑1 (MQ=60)

                                        CGCGATTCACCCCGCCGTACAGGTAAAAGTGCAGGGTTAATCCAGGC‑AAAAAACGCTACAAAAATGCCCGATCATCGATCGGGCATTTGGACTT                                                     <  SRR4302536.1909152/94‑1 (MQ=46)

                                        CGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTT                                                     <  SRR4302536.1620629/94‑1 (MQ=60)

                                         GCGAAGCACCCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTT                                                    <  SRR4302536.4052358/94‑1 (MQ=60)

                                          CGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                   <  SRR4302536.4202384/94‑1 (MQ=60)

                                          CGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                   <  SRR4302536.2856393/94‑1 (MQ=60)

                                          CGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTT                                                   >  SRR4302536.6541566/1‑94 (MQ=60)

                                           GAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTA                                                  <  SRR4302536.1114118/94‑1 (MQ=60)

                                             AGCACGCGGCGGGACAGGTAAAAGTACGGGGTTAAGCCCGGC‑AAAAAACGCTACAAAAGTGCCCGATCCTCGATCGGGCATTTTGACTTTTACA                                                <  SRR4302536.2963551/94‑1 (MQ=60)

                                             AGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACA                                                <  SRR4302536.2342493/94‑1 (MQ=60)

                                             AGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACA                                                <  SRR4302536.2182323/94‑1 (MQ=60)

                                              GCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAG                                               <  SRR4302536.2214454/94‑1 (MQ=60)

                                               CACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCCCGATCGGGCGTTTTGACCTTTACAGC                                              >  SRR4302536.4905073/1‑94 (MQ=60)

                                                 CGCCGCGGGACAGGTAAAAGTACAGGGTTAAGCCCGGC‑AAAAAGCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTT                                            <  SRR4302536.787271/94‑1 (MQ=60)

                                                 CGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTT                                            <  SRR4302536.1108262/94‑1 (MQ=60)

                                                 CGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATCCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTT                                            <  SRR4302536.2689728/94‑1 (MQ=60)

                                                  GCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTGTACAGCTTA                                           <  SRR4302536.6719103/94‑1 (MQ=60)

                                                   CCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAG                                          <  SRR4302536.3193613/94‑1 (MQ=60)

                                                    CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGGCCGATCCTCGATCGGGCATTTTGAATTTTACAGCTTAGC                                         >  SRR4302536.2486971/1‑94 (MQ=60)

                                                    CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGC                                         <  SRR4302536.3006081/94‑1 (MQ=60)

                                                    CGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCCATCGGGCCTTTTTTCTTTTTTAGCTTAGC                                         >  SRR4302536.4819791/1‑94 (MQ=60)

                                                      CCGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGC                                       >  SRR4302536.3455467/1‑94 (MQ=60)

                                                       CGGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCC                                      >  SRR4302536.444798/1‑94 (MQ=60)

                                                        GGACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCT                                     >  SRR4302536.3445970/1‑94 (MQ=60)

                                                         GACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTT                                    <  SRR4302536.4657330/94‑1 (MQ=60)

                                                          ACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCTGGCATTTTGACTTTTACAGCTTAGCGCCTTC                                   >  SRR4302536.2652173/1‑94 (MQ=60)

                                                          ACAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTC                                   <  SRR4302536.2917853/94‑1 (MQ=60)

                                                           CAGGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACTCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCT                                  <  SRR4302536.2055772/94‑1 (MQ=60)

                                                           AAGGTAAAAGTACAGGGTAAACCCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCT                                  <  SRR4302536.4689150/94‑1 (MQ=60)

                                                             GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAC                                <  SRR4302536.4085654/94‑1 (MQ=60)

                                                             GGTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTAC                                >  SRR4302536.3309069/1‑94 (MQ=60)

                                                              GTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTATAGCTTATCGCCTTCTTCA                               >  SRR4302536.1184589/1‑94 (MQ=60)

                                                              GTAAAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACA                               <  SRR4302536.3101039/94‑1 (MQ=60)

                                                                 AAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTTCAACTTATCGCCTTCTACAGCT                            >  SRR4302536.2483433/1‑94 (MQ=60)

                                                                 AAAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCT                            >  SRR4302536.6814703/1‑94 (MQ=60)

                                                                  AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           >  SRR4302536.5067158/1‑94 (MQ=60)

                                                                  AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           >  SRR4302536.648716/1‑94 (MQ=60)

                                                                  AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           >  SRR4302536.4235807/1‑94 (MQ=60)

                                                                  AAGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTT                           <  SRR4302536.4120209/94‑1 (MQ=60)

                                                                   AGTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTC                          >  SRR4302536.3381098/1‑94 (MQ=60)

                                                                   AGTACAGGGTT‑AACCCCGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTC                          <  SRR4302536.2565114/94‑1 (MQ=60)

                                                                    GTACAGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCA                         <  SRR4302536.1006236/94‑1 (MQ=60)

                                                                    GTAAAGGGTAAAACCCGGC‑AAAAAACGCTAAAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCA                         <  SRR4302536.1323057/94‑1 (MQ=60)

                                                                     TACAGGGTAAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCAC                        <  SRR4302536.4828649/94‑1 (MQ=60)

                                                                        AGGGTTTAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                     >  SRR4302536.4471389/1‑94 (MQ=60)

                                                                        AGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                     <  SRR4302536.3267642/94‑1 (MQ=60)

                                                                        AGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                     >  SRR4302536.1555631/1‑94 (MQ=60)

                                                                        AGGGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCG                     >  SRR4302536.2886876/1‑94 (MQ=60)

                                                                          GGTTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCC                   >  SRR4302536.5763260/1‑94 (MQ=60)

                                                                            TTAAACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAG                 >  SRR4302536.5371358/1‑94 (MQ=60)

                                                                               AACCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTTCAGCTTAGCGCCCTCTACAGCTTtccgcccccgctt              >  SRR4302536.1318305/1‑81 (MQ=60)

                                                                                 CCCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG            <  SRR4302536.2147789/94‑1 (MQ=60)

                                                                                  cccagcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAG            <  SRR4302536.1129311/88‑1 (MQ=60)

                                                                                  CCGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGT           <  SRR4302536.6332932/94‑1 (MQ=60)

                                                                                   CGGC‑AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTA          <  SRR4302536.5403804/94‑1 (MQ=60)

                                                                                      gcAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAAT        <  SRR4302536.4209152/92‑1 (MQ=60)

                                                                                        AAAAAACGCTACAAAAATGCCCGATCCTCGGTCGGGCATTTTGTCTTTTACAGCTTTGCGCCTTCTACAGCTTCACtagctggcttagtaatgg      >  SRR4302536.6431331/1‑76 (MQ=60)

                                                                                        AAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGC      >  SRR4302536.275894/1‑94 (MQ=60)

                                                                                         AAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCG     >  SRR4302536.2036588/1‑94 (MQ=60)

                                                                                          AAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGG    <  SRR4302536.3699547/94‑1 (MQ=60)

                                                                                           AAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTTAATTTTTTAGCTTAGCGCCTTCTACAGCTTCACGCGCCCGCCTAGTAATGCGGC   >  SRR4302536.117773/1‑94 (MQ=60)

                                                                                           AAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGT   >  SRR4302536.6849652/1‑94 (MQ=60)

                                                                                           AAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGG   >  SRR4302536.5360411/1‑94 (MQ=60)

                                                                                            AACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTTCAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGGC  >  SRR4302536.5058085/1‑94 (MQ=60)

                                                                                            AACGCTACAAAAATGCCCGATCCTCGATCCGGCCTTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  >  SRR4302536.5512222/1‑94 (MQ=60)

CACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTC  >  NC_000913/1930004‑1930189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`