Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,941,713	T→C	S92S (AGT→AGC)	yebI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,941,713	0	T	C	99.6%	709.5 / ‑4.7	223	S92S (AGT→AGC)	yebI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (92/130); total (92/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 228 total aligned reads displayed.

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

 ttACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGccc                                                                                       <  1:6474217/93‑1 (MQ=255)

 ttACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGccc                                                                                       <  1:3492393/93‑1 (MQ=255)

  tACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCt                                                                                      >  2:3156433/1‑93 (MQ=255)

   aCGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTg                                                                                     >  2:6632149/1‑93 (MQ=255)

    cGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGCAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCTCCCTGt                                                                                    <  2:2070626/93‑1 (MQ=255)

     gctgctgctGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTc                                                                                   <  2:6327155/93‑1 (MQ=255)

      ctgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCg                                                                                  <  1:6013202/93‑1 (MQ=255)

       tgctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGc                                                                                 <  1:5963622/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                >  1:1801268/1‑93 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                <  2:4682344/93‑1 (MQ=255)

        gctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCt                                                                                >  2:2257766/1‑93 (MQ=255)

         ctgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTg                                                                               <  2:4856039/93‑1 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              >  1:1702294/1‑93 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              <  1:4759255/93‑1 (MQ=255)

          tgctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTgg                                                                              >  1:6120634/1‑93 (MQ=255)

            ctgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGc                                                                            <  1:3080899/93‑1 (MQ=255)

             tgGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGcc                                                                           <  1:2790774/93‑1 (MQ=255)

             tcgcgggcggTCTGGTATGGCTGGACAACCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGcc                                                                           <  1:73044/91‑1 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGTCt                                                                          >  2:219804/1‑93 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCt                                                                          >  2:2630622/1‑93 (MQ=255)

              ggcgggcgGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCt                                                                          >  2:3880531/1‑93 (MQ=255)

                cgggcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtgg                                                                        <  2:5944954/93‑1 (MQ=255)

                   gcggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCtggtgg                                                                     <  1:5576086/93‑1 (MQ=255)

                    cggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTggtggt                                                                    <  2:6430975/93‑1 (MQ=255)

                     ggTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTc                                                                   >  2:3485685/1‑93 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCGACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               <  1:2675610/93‑1 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCTCTGGTCCTGGAGGTCGTGa                                                               <  1:6563744/93‑1 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               >  2:1414087/1‑93 (MQ=255)

                         tGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGa                                                               <  1:4488054/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAg                                                              <  1:3334994/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              <  1:5185101/93‑1 (MQ=255)

                          ggTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATGAGGGATTATGGCGCCCAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAg                                                              <  2:3216428/93‑1 (MQ=255)

                            tATGGATGGAGAAACGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            <  2:1814082/93‑1 (MQ=255)

                            gaTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTc                                                            <  2:4434115/92‑1 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGGCTGGTGGTCGTGAGTCt                                                           >  2:2356155/1‑93 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCt                                                           <  1:1294460/93‑1 (MQ=255)

                             aTGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCt                                                           >  1:2112429/1‑93 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGGTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGAt                                                        <  1:3481639/93‑1 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGAt                                                        <  1:474198/93‑1 (MQ=255)

                                gCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGAt                                                        <  1:1768121/93‑1 (MQ=255)

                                 cTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATg                                                       <  2:5771519/93‑1 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     >  1:5490566/1‑93 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     <  1:1667343/93‑1 (MQ=255)

                                   ggAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTc                                                     <  2:3086444/93‑1 (MQ=255)

                                     agaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTa                                                   >  1:2099471/1‑93 (MQ=255)

                                      gaAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTaa                                                  <  2:4956639/93‑1 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                >  1:1458937/1‑93 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                <  2:2891502/93‑1 (MQ=255)

                                        aGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAata                                                <  2:3774646/93‑1 (MQ=255)

                                         gCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAatat                                               >  2:35708/1‑93 (MQ=255)

                                             ccACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgt                                           >  1:5857687/1‑93 (MQ=255)

                                               acaGCTGGCGATCGACACGTAATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCgtgt                                         <  1:758576/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        <  1:5348026/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        >  1:6246812/1‑93 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        <  1:6515056/93‑1 (MQ=255)

                                                caGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGtt                                        >  1:3941900/1‑93 (MQ=255)

                                                 aGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttg                                       <  1:6401108/93‑1 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      >  1:6713435/1‑93 (MQ=255)

                                                  gCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttga                                      <  1:3544092/93‑1 (MQ=255)

                                                    tGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCCGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatt                                    >  1:2921307/1‑93 (MQ=255)

                                                     ggCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgattt                                   <  1:3996836/93‑1 (MQ=255)

                                                     ggCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgattt                                   <  2:2230357/93‑1 (MQ=255)

                                                      gCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttg                                  <  2:2808196/93‑1 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 <  2:4740799/93‑1 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 >  2:447337/1‑93 (MQ=255)

                                                       cGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttga                                 <  1:4055913/93‑1 (MQ=255)

                                                        gATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttgat                                <  2:5222877/93‑1 (MQ=255)

                                                        gATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGttgatttgat                                >  2:4006893/1‑93 (MQ=255)

                                                         aTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATg                               <  2:6199927/93‑1 (MQ=255)

                                                          tCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATgg                              >  2:1288168/1‑93 (MQ=255)

                                                           cGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGACTAATATTCGTGTTGATTTGCTGGc                             >  1:1084451/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  2:1020150/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  2:452655/1‑93 (MQ=255)

                                                            gACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCt                            >  1:4188455/1‑93 (MQ=255)

                                                             acacGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCtt                           <  2:836085/93‑1 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  1:5907344/1‑93 (MQ=255)

                                                               acGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAc                         >  2:1707255/1‑93 (MQ=255)

                                                                cGTTATTAGGGATTGTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAcc                        <  1:5006120/93‑1 (MQ=255)

                                                                 gTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTTATATTCGTGTTGATTTGATGGCTTACCt                       >  2:1166551/1‑93 (MQ=255)

                                                                  ttattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTg                      >  2:3954464/1‑93 (MQ=255)

                                                                  ttattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTg                      >  2:4338372/1‑93 (MQ=255)

                                                                   tattaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTCCCTGt                     >  2:5191521/1‑93 (MQ=255)

                                                                    attaGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGtt                    <  2:5494619/93‑1 (MQ=255)

                                                                    attaGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGtt                    >  1:5092573/1‑93 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                >  1:2148564/1‑93 (MQ=255)

                                                                        gggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGt                >  1:3183898/1‑93 (MQ=255)

                                                                         ggATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTg               >  1:4814627/1‑93 (MQ=255)

                                                                          gATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGa              <  1:4560096/93‑1 (MQ=255)

                                                                           aTTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAt             >  1:81307/1‑93 (MQ=255)

                                                                            ttATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAtt            <  1:1951156/93‑1 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          >  1:4915658/1‑93 (MQ=255)

                                                                              aTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTg          <  1:5727910/93‑1 (MQ=255)

                                                                               tGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGc         >  1:4368800/1‑93 (MQ=255)

                                                                                ggCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCt        >  1:2565889/1‑93 (MQ=255)

                                                                                 gcgcACAGCTCCCTGTCGCTGGGCCTGGTGTTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  1:2448664/93‑1 (MQ=255)

                                                                                 gcgcACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  2:3446310/93‑1 (MQ=255)

                                                                                 gcgcACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTg       <  2:731257/93‑1 (MQ=255)

                                                                                    cacaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCa    <  1:531453/93‑1 (MQ=255)

                                                                                     acaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAg   <  2:4612698/93‑1 (MQ=255)

                                                                                     acaGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAg   >  2:5584117/1‑93 (MQ=255)

GTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAGT  >  NC_000913/1941624‑1941802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGCCGTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAG  >  NC_000913/1941620‑1941801

TGCCGTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGC                                                                                          <  SRR4302536.370486/94‑1 (MQ=60)

   CGTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGGTATTAGGGATTATGGCGCACAGCGCC                                                                                       >  SRR4302536.3282583/1‑94 (MQ=60)

     TTACGCTGCTGCTGGGGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCT                                                                                     <  SRR4302536.1905199/94‑1 (MQ=60)

     TTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCT                                                                                     <  SRR4302536.3596579/94‑1 (MQ=60)

     TTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCT                                                                                     <  SRR4302536.6667429/94‑1 (MQ=60)

      TACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTG                                                                                    >  SRR4302536.1161530/1‑94 (MQ=60)

        CGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATGAGGGATTATGGCGCACAGCGCCCTGTC                                                                                  <  SRR4302536.3296698/94‑1 (MQ=60)

          CTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGC                                                                                <  SRR4302536.6192469/94‑1 (MQ=60)

           TGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCT                                                                               <  SRR4302536.6141361/94‑1 (MQ=60)

           TGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCT                                                                               >  SRR4302536.1855643/1‑94 (MQ=60)

           TGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCT                                                                               <  SRR4302536.620196/94‑1 (MQ=60)

            GCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTG                                                                              >  SRR4302536.2895365/1‑94 (MQ=60)

             CTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGG                                                                             >  SRR4302536.5857382/1‑94 (MQ=60)

             CTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGG                                                                             >  SRR4302536.1751557/1‑94 (MQ=60)

             CTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGG                                                                             >  SRR4302536.6303065/1‑94 (MQ=60)

              TGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGG                                                                            <  SRR4302536.223731/94‑1 (MQ=60)

              TGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGG                                                                            <  SRR4302536.4902560/94‑1 (MQ=60)

              TGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGACCACAGCTGGCTATCGACACGTTATTAGGGATTATGGCACACAGCGCCCGGTCGCTGGG                                                                            <  SRR4302536.6725835/94‑1 (MQ=60)

                CTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCC                                                                          <  SRR4302536.3171064/94‑1 (MQ=60)

                CTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCC                                                                          <  SRR4302536.5090381/94‑1 (MQ=60)

                 tggcggctgtTCTGGTATGGCTAGAAGAGCATCCACAGATGTCGATCGACACGATATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCT                                                                         <  SRR4302536.2255505/84‑1 (MQ=37)

                 TGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCT                                                                         <  SRR4302536.4775170/94‑1 (MQ=60)

                 TGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCT                                                                         <  SRR4302536.2873606/94‑1 (MQ=60)

                 TCGCGGGCGGTCTGGTATGGCTGGACAACCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCT                                                                         <  SRR4302536.75014/94‑1 (MQ=60)

                  GGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTG                                                                        >  SRR4302536.656351/1‑94 (MQ=60)

                    CGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGT                                                                      >  SRR4302536.2146634/1‑94 (MQ=60)

                     GGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTG                                                                     <  SRR4302536.5668961/94‑1 (MQ=60)

                       GCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGT                                                                   <  SRR4302536.5742063/94‑1 (MQ=60)

                        CGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGNACNNCGCCCTGTCGCTGGNCNNGGTGGTC                                                                  >  SRR4302536.6623751/1‑94 (MQ=60)

                         GGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCG                                                                 <  SRR4302536.6181521/94‑1 (MQ=60)

                          GTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGG                                                                >  SRR4302536.2368621/1‑94 (MQ=60)

                           TCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTG                                                               <  SRR4302536.140140/94‑1 (MQ=60)

                            CTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGA                                                              <  SRR4302536.4315839/94‑1 (MQ=60)

                             TGGTATGGCTGGAGAAGCGTCGACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAG                                                             <  SRR4302536.2755582/94‑1 (MQ=60)

                             TGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCTCTGGTCCTGGAGGTCGTGAG                                                             <  SRR4302536.6759133/94‑1 (MQ=60)

                             TGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAG                                                             <  SRR4302536.4622829/94‑1 (MQ=60)

                              GGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGT                                                            <  SRR4302536.3433753/94‑1 (MQ=60)

                              GGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGT                                                            <  SRR4302536.5339755/94‑1 (MQ=60)

                              GGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGT                                                            >  SRR4302536.880371/1‑94 (MQ=60)

                               GTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTC                                                           >  SRR4302536.6041332/1‑94 (MQ=60)

                                TATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCT                                                          >  SRR4302536.2175914/1‑94 (MQ=60)

                                 ATGTCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGTTTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTG                                                         <  SRR4302536.5534973/94‑1 (MQ=60)

                                 ATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACATGTTATTAGGTATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTG                                                         <  SRR4302536.3444321/94‑1 (MQ=60)

                                 ATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTG                                                         >  SRR4302536.4240466/1‑94 (MQ=60)

                                 ATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTG                                                         <  SRR4302536.5257234/94‑1 (MQ=60)

                                 ATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTG                                                         <  SRR4302536.1333161/94‑1 (MQ=60)

                                  TGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGA                                                        >  SRR4302536.6489877/1‑94 (MQ=60)

                                  TGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGA                                                        >  SRR4302536.4240988/1‑94 (MQ=60)

                                  TGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGA                                                        <  SRR4302536.6815889/94‑1 (MQ=60)

                                   GGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGAT                                                       <  SRR4302536.5167295/94‑1 (MQ=60)

                                   GGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGAT                                                       >  SRR4302536.667053/1‑94 (MQ=60)

                                   GGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGAT                                                       <  SRR4302536.4173498/94‑1 (MQ=60)

                                    GCTGGAGAAGCGTCCACAGCTGGCGGTCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATG                                                      <  SRR4302536.3585446/94‑1 (MQ=60)

                                    GCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATG                                                      <  SRR4302536.486546/94‑1 (MQ=60)

                                    GCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATG                                                      <  SRR4302536.1821632/94‑1 (MQ=60)

                                      ttaatgatgcggcgaccaccgagatctACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGGTGGTCCTGGTGGTCGTGAGGCTGATGGC                                                    <  SRR4302536.2914330/67‑1 (MQ=60)

                                      TGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTC                                                    >  SRR4302536.5654447/1‑94 (MQ=60)

                                       GGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCT                                                   <  SRR4302536.1715716/94‑1 (MQ=60)

                                        GAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTA                                                  >  SRR4302536.1843490/1‑94 (MQ=60)

                                        GAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTA                                                  >  SRR4302536.2162615/1‑94 (MQ=60)

                                         AGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAA                                                 <  SRR4302536.2693716/94‑1 (MQ=60)

                                           AAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATA                                               <  SRR4302536.173216/94‑1 (MQ=60)

                                           AAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATA                                               >  SRR4302536.1502120/1‑94 (MQ=60)

                                            AGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATAT                                              <  SRR4302536.2794917/94‑1 (MQ=60)

                                              CGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTC                                            >  SRR4302536.2096281/1‑94 (MQ=60)

                                               GTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCG                                           >  SRR4302536.3698637/1‑94 (MQ=60)

                                               GTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCG                                           >  SRR4302536.752690/1‑94 (MQ=60)

                                                TCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGT                                          >  SRR4302536.6032198/1‑94 (MQ=60)

                                                  CACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGT                                        <  SRR4302536.4669518/94‑1 (MQ=60)

                                                   ACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTT                                       >  SRR4302536.4058999/1‑94 (MQ=60)

                                                   ACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTT                                       >  SRR4302536.6432892/1‑94 (MQ=60)

                                                   ACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTT                                       >  SRR4302536.4908829/1‑94 (MQ=60)

                                                   ACAGCTGGCGATCGACACGTAATTAGGGATTATGGCGCACAGCGCCATGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTT                                       <  SRR4302536.780815/94‑1 (MQ=60)

                                                    CAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTG                                      <  SRR4302536.5507709/94‑1 (MQ=60)

                                                    CAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTG                                      <  SRR4302536.6709185/94‑1 (MQ=60)

                                                    CAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTG                                      <  SRR4302536.2585705/94‑1 (MQ=60)

                                                    CAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTG                                      >  SRR4302536.3665001/1‑94 (MQ=60)

                                                    CAGCTGGCGATAGACACGTGATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTG                                      <  SRR4302536.3060362/94‑1 (MQ=60)

                                                     AGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGT                                     >  SRR4302536.3646123/1‑94 (MQ=60)

                                                     AGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGA                                     <  SRR4302536.6592163/94‑1 (MQ=60)

                                                     AGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGA                                     >  SRR4302536.6112941/1‑94 (MQ=60)

                                                     AGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGA                                     >  SRR4302536.6912742/1‑94 (MQ=60)

                                                     AGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGA                                     <  SRR4302536.3562060/94‑1 (MQ=60)

                                                      GCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGAT                                    <  SRR4302536.3650055/94‑1 (MQ=60)

                                                       CTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCCGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATT                                   >  SRR4302536.3007522/1‑94 (MQ=60)

                                                        TGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCGCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTTATATTCGTGTTGATTT                                  >  SRR4302536.786914/1‑94 (MQ=60)

                                                        TGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTT                                  <  SRR4302536.1697066/94‑1 (MQ=60)

                                                        TGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTT                                  >  SRR4302536.4112511/1‑94 (MQ=60)

                                                        TGGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGATCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTT                                  <  SRR4302536.5100961/94‑1 (MQ=60)

                                                         GGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTG                                 <  SRR4302536.660200/94‑1 (MQ=60)

                                                         GGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTG                                 <  SRR4302536.5374806/94‑1 (MQ=60)

                                                         GGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTG                                 <  SRR4302536.4115443/94‑1 (MQ=60)

                                                         GGCGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGCCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTG                                 <  SRR4302536.6915976/94‑1 (MQ=60)

                                                           CGATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGAT                               <  SRR4302536.4176141/94‑1 (MQ=60)

                                                            GATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATG                              >  SRR4302536.827386/1‑94 (MQ=60)

                                                            GATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATG                              <  SRR4302536.5795024/94‑1 (MQ=60)

                                                            GATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATG                              <  SRR4302536.5929999/94‑1 (MQ=60)

                                                            GATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATG                              <  SRR4302536.2178821/94‑1 (MQ=60)

                                                            CATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGCCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATG                              <  SRR4302536.3196825/94‑1 (MQ=60)

                                                             ATCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGG                             >  SRR4302536.608062/1‑94 (MQ=60)

                                                              TCGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGACTAATATTCGTGTTGATTTGCTGGC                            >  SRR4302536.1116933/1‑94 (MQ=60)

                                                               CGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCT                           <  SRR4302536.634982/94‑1 (MQ=60)

                                                               CGACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCT                           >  SRR4302536.4312909/1‑94 (MQ=60)

                                                                GACACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTT                          <  SRR4302536.4761832/94‑1 (MQ=60)

                                                                  CACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAC                        <  SRR4302536.4894654/94‑1 (MQ=60)

                                                                  CACGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTAC                        >  SRR4302536.6083288/1‑94 (MQ=60)

                                                                    CGTTATTAGGGATTGTGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCT                      <  SRR4302536.5156301/94‑1 (MQ=60)

                                                                    CGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCT                      <  SRR4302536.6718256/94‑1 (MQ=60)

                                                                    CGTTATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCT                      <  SRR4302536.3004069/94‑1 (MQ=60)

                                                                       TATTAGGTATTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTT                   <  SRR4302536.283320/94‑1 (MQ=60)

                                                                       TATTAGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTT                   >  SRR4302536.5244868/1‑94 (MQ=60)

                                                                           AGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGT               >  SRR4302536.3277252/1‑94 (MQ=60)

                                                                           AGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGT               >  SRR4302536.2213070/1‑94 (MQ=60)

                                                                           AGGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGG               >  SRR4302536.1735856/1‑94 (MQ=60)

                                                                            GGGATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTG              >  SRR4302536.4959488/1‑94 (MQ=60)

                                                                              GATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAT            <  SRR4302536.4696906/94‑1 (MQ=60)

                                                                              GATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAT            >  SRR4302536.83491/1‑94 (MQ=60)

                                                                              GATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGGCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGAT            >  SRR4302536.4532700/1‑94 (MQ=60)

                                                                               ATTATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATT           >  SRR4302536.5568680/1‑94 (MQ=60)

                                                                                TTATGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTT          <  SRR4302536.2009837/94‑1 (MQ=60)

                                                                                 TATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTG         >  SRR4302536.5063399/1‑94 (MQ=60)

                                                                                 TATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTG         <  SRR4302536.5500675/94‑1 (MQ=60)

                                                                                 TATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTG         <  SRR4302536.5419029/94‑1 (MQ=60)

                                                                                  ATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGC        <  SRR4302536.5898266/94‑1 (MQ=60)

                                                                                  ATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGC        >  SRR4302536.3594488/1‑94 (MQ=60)

                                                                                  ATGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGC        >  SRR4302536.4499326/1‑94 (MQ=60)

                                                                                   TGGCGCACAGCGCCCTGTCGCTGGTCCTGGTGGTCGTGAGTCTGATGCCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCT       <  SRR4302536.2171187/94‑1 (MQ=60)

                                                                                   TGGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCT       >  SRR4302536.2643084/1‑94 (MQ=60)

                                                                                    GGCGCACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTG      <  SRR4302536.5056656/94‑1 (MQ=60)

                                                                                     GCGCACAGCTCCCTGTCGCTGGGCCTGGTGTTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGG     <  SRR4302536.2522642/94‑1 (MQ=60)

                                                                                      CGCACAGCGCCCTGTCGCTGGGCCTGGGGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGC    >  SRR4302536.1783462/1‑94 (MQ=60)

                                                                                        CACAGCGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAG  <  SRR4302536.545045/94‑1 (MQ=60)

TGCCGTTACGCTGCTGCTGGCGGGCGGTCTGGTATGGCTGGAGAAGCGTCCACAGCTGGCGATCGACACGTTATTAGGGATTATGGCGCACAGTGCCCTGTCGCTGGGCCTGGTGGTCGTGAGTCTGATGTCTAATATTCGTGTTGATTTGATGGCTTACCTGTTCGGTGATTTGCTGGCAG  >  NC_000913/1941620‑1941801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`