Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,983,890 T→C T88A (ACT→GCT)  araF ← L‑arabinose‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,983,8900TC100.0% 704.0 / NA 215T88A (ACT→GCT) araFL‑arabinose‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (115/100);  total (115/100)

Only 100 of 221 total aligned reads displayed.

GTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA  >  NC_000913/1983801‑1983979
                                                                                         |                                                                                         
gTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGcaca                                                                                        >  1:293753/1‑93 (MQ=255)
gTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGcaca                                                                                        >  2:1566944/1‑93 (MQ=255)
 ttAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACaa                                                                                       >  1:4576706/1‑93 (MQ=255)
 ttAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACaa                                                                                       >  2:2393177/1‑93 (MQ=255)
  tAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACaaa                                                                                      >  1:3756498/1‑93 (MQ=255)
   aaCAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAAt                                                                                     <  2:5391547/93‑1 (MQ=255)
    aCAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATa                                                                                    >  2:6042475/1‑93 (MQ=255)
    aCAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATa                                                                                    >  1:845222/1‑93 (MQ=255)
      aaaCTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAc                                                                                  >  1:1362879/1‑93 (MQ=255)
         cTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGaa                                                                               <  2:3776714/93‑1 (MQ=255)
         cTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGaa                                                                               >  2:6710556/1‑93 (MQ=255)
          tGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGGACAAATAACGaaa                                                                              >  1:4113474/1‑93 (MQ=255)
          tGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGaaa                                                                              >  2:3676802/1‑93 (MQ=255)
           ggTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAc                                                                             <  2:5345270/93‑1 (MQ=255)
            gTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAcc                                                                            >  2:3963755/1‑93 (MQ=255)
            gTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAAcc                                                                            <  1:302913/93‑1 (MQ=255)
             tcttCCCCGGCAATGACTTTCATATCGTCGCCCCGCGCTTTCGCGACGATGGCAGAGCTGAGTTTGGGGTCCGGAGCACAAATAACGAAACCt                                                                           <  1:3560407/90‑1 (MQ=255)
             tCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCt                                                                           <  2:5054299/93‑1 (MQ=255)
              cATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtt                                                                          >  2:4412586/1‑93 (MQ=255)
              cATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtt                                                                          >  1:5252995/1‑93 (MQ=255)
               aTCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCttt                                                                         >  2:6135195/1‑93 (MQ=255)
               aTCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCttt                                                                         <  1:6696634/93‑1 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        <  1:2977867/93‑1 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        >  1:2637468/1‑93 (MQ=255)
                tCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCtttt                                                                        <  1:3731320/93‑1 (MQ=255)
                 ccACGGCAATGTCTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTg                                                                       <  1:647099/93‑1 (MQ=255)
                 ccACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTg                                                                       <  1:3103089/93‑1 (MQ=255)
                  cACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGATACCTTTTgc                                                                      <  1:1648858/93‑1 (MQ=255)
                  cACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTgc                                                                      <  1:46669/93‑1 (MQ=255)
                   aCGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTgcg                                                                     >  1:5939439/1‑93 (MQ=255)
                     ggCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGcc                                                                   <  2:1284941/93‑1 (MQ=255)
                      gCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCa                                                                  <  1:6687847/93‑1 (MQ=255)
                        aaTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACt                                                                >  2:2316065/1‑93 (MQ=255)
                        aaTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACt                                                                >  2:6304626/1‑93 (MQ=255)
                         aTGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTg                                                               <  1:3056987/93‑1 (MQ=255)
                          tGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTgg                                                              >  1:3436871/1‑93 (MQ=255)
                           gACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGc                                                             >  1:1195244/1‑93 (MQ=255)
                           gACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGc                                                             <  1:2363269/93‑1 (MQ=255)
                            aCTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCa                                                            <  1:2595905/93‑1 (MQ=255)
                             cTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAg                                                           >  2:3352836/1‑93 (MQ=255)
                               ttCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGcc                                                         >  1:1432356/1‑93 (MQ=255)
                               ttCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGcc                                                         <  1:5059898/93‑1 (MQ=255)
                               ttCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGcc                                                         <  1:981768/93‑1 (MQ=255)
                                tCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCa                                                        <  2:6208994/93‑1 (MQ=255)
                                  atatCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAgg                                                      >  1:5964671/1‑93 (MQ=255)
                                   tatCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGc                                                     >  2:2931181/1‑93 (MQ=255)
                                    atCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCt                                                    <  1:3504796/93‑1 (MQ=255)
                                      cGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGt                                                  <  2:1602575/93‑1 (MQ=255)
                                      cGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGt                                                  <  1:5541958/93‑1 (MQ=255)
                                         aGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGa                                               <  2:5806026/93‑1 (MQ=255)
                                         aGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGa                                               >  1:2666127/1‑93 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              >  1:5133179/1‑93 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              >  2:1467478/1‑93 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              >  2:4729011/1‑93 (MQ=255)
                                          gCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAt                                              <  2:3160024/93‑1 (MQ=255)
                                           ccACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATc                                             <  1:2186254/93‑1 (MQ=255)
                                              cgcgCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATcgcg                                          >  2:1838604/1‑93 (MQ=255)
                                                cgcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGtt                                        <  2:4673197/93‑1 (MQ=255)
                                                 gcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTc                                       >  2:3539697/1‑93 (MQ=255)
                                                 gcTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTc                                       <  1:4052227/93‑1 (MQ=255)
                                                  cTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCa                                      >  2:1667398/1‑93 (MQ=255)
                                                   tttCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCaa                                     >  1:1716990/1‑93 (MQ=255)
                                                    ttCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAAt                                    <  1:1887231/93‑1 (MQ=255)
                                                    ttCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAAt                                    <  1:1473726/93‑1 (MQ=255)
                                                      cgcgACGATGGCAGAGCCGAGTTTGGGTTCCGGAGCACAAATAACGAAACCTTTTGCCCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGt                                  <  1:580804/93‑1 (MQ=255)
                                                      cgcgACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGt                                  <  1:2648871/93‑1 (MQ=255)
                                                      cgcgACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCACGCTGTCGATCGCGTTCAATGt                                  <  1:4238924/93‑1 (MQ=255)
                                                       gcgACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtt                                 <  2:4333100/93‑1 (MQ=255)
                                                         gacgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttt                               >  2:5742003/1‑93 (MQ=255)
                                                          acgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGttttt                              <  2:189340/93‑1 (MQ=255)
                                                           cgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttttt                             >  2:5603801/1‑93 (MQ=255)
                                                           cgaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGtttttt                             >  1:699493/1‑93 (MQ=255)
                                                            gaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTc                            <  1:2160874/93‑1 (MQ=255)
                                                            gaTGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTc                            <  1:3425972/93‑1 (MQ=255)
                                                               ggCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGcc                         <  2:219294/93‑1 (MQ=255)
                                                               ggCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGcc                         <  1:6377920/93‑1 (MQ=255)
                                                                gCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCa                        >  2:2571002/1‑93 (MQ=255)
                                                                  agagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATc                      >  1:65855/1‑93 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     >  1:5770837/1‑93 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     <  1:604797/93‑1 (MQ=255)
                                                                   gagCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATcc                     >  2:5007800/1‑93 (MQ=255)
                                                                    agCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcg                    <  2:5140472/93‑1 (MQ=255)
                                                                         aGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacg               >  2:3986753/1‑93 (MQ=255)
                                                                          gTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGCTCGCGATCAATGTTTTTTCGCCCTCcggcacgg              >  1:5607529/1‑93 (MQ=255)
                                                                          gTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacgg              >  2:94006/1‑93 (MQ=255)
                                                                           tttGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCcggcacggc             >  2:563949/1‑93 (MQ=255)
                                                                             tGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCaa           <  2:5287287/93‑1 (MQ=255)
                                                                             tGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCaa           <  2:6515023/93‑1 (MQ=255)
                                                                               gggTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATc         <  2:667046/93‑1 (MQ=255)
                                                                               gggTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATc         >  1:125395/1‑93 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCtt       >  2:2819405/1‑93 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCtt       >  2:1726463/1‑93 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCtt       >  1:6286775/1‑93 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCtt       >  1:622120/1‑93 (MQ=255)
                                                                                 gTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACCGCAATCtt       >  2:3433796/1‑93 (MQ=255)
                                                                                  gccGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTta      <  2:1357009/92‑1 (MQ=255)
                                                                                    cGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaat    <  2:506887/93‑1 (MQ=255)
                                                                                    cGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaat    <  2:233619/93‑1 (MQ=255)
                                                                                     ggAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaata   <  1:4721814/93‑1 (MQ=255)
                                                                                      gAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTtaataa  >  1:6171487/1‑93 (MQ=255)
                                                                                         |                                                                                         
GTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA  >  NC_000913/1983801‑1983979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAACC  >  NC_000913/1983798‑1983981
                                                                                            |                                                                                           
GGCGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCA                                                                                            >  SRR4302536.375426/1‑94 (MQ=60)
GGCGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCA                                                                                            <  SRR4302536.6553633/94‑1 (MQ=60)
 GCGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCAC                                                                                           <  SRR4302536.2883931/94‑1 (MQ=60)
  CGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACA                                                                                          >  SRR4302536.301561/1‑94 (MQ=60)
   GTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAA                                                                                         >  SRR4302536.4714079/1‑94 (MQ=60)
    TTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAA                                                                                        >  SRR4302536.3868720/1‑94 (MQ=60)
     TAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAAT                                                                                       >  SRR4302536.2392336/1‑94 (MQ=60)
      AACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATA                                                                                      >  SRR4302536.870071/1‑94 (MQ=60)
      AACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATA                                                                                      <  SRR4302536.6020337/94‑1 (MQ=60)
      AACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGATTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATA                                                                                      >  SRR4302536.1330099/1‑94 (MQ=60)
       ACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAA                                                                                     <  SRR4302536.1472482/94‑1 (MQ=60)
        CAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAC                                                                                    >  SRR4302536.5315019/1‑94 (MQ=60)
        CAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAC                                                                                    <  SRR4302536.4845531/94‑1 (MQ=60)
        CAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAC                                                                                    <  SRR4302536.4739201/94‑1 (MQ=60)
        CAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAC                                                                                    >  SRR4302536.4497607/1‑94 (MQ=60)
        CAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAAC                                                                                    >  SRR4302536.1403503/1‑94 (MQ=60)
         AAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACG                                                                                   >  SRR4302536.6267107/1‑94 (MQ=60)
         AAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACG                                                                                   >  SRR4302536.342152/1‑94 (MQ=60)
         AAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACG                                                                                   >  SRR4302536.5802276/1‑94 (MQ=60)
          AACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGA                                                                                  >  SRR4302536.911349/1‑94 (MQ=60)
            CTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGGACAAATAACGAAA                                                                                >  SRR4302536.4235496/1‑94 (MQ=60)
               GTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCT                                                                             >  SRR4302536.2749254/1‑94 (MQ=60)
               GTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCT                                                                             <  SRR4302536.310964/94‑1 (MQ=60)
                TCTTCCCCGGCAATGACTTTCATATCGTCGCCCCGCGCTTTCGCGACGATGGCAGAGCTGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTT                                                                            <  SRR4302536.3666854/94‑1 (MQ=60)
                TCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTT                                                                            >  SRR4302536.5409515/1‑94 (MQ=60)
                 CATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTT                                                                           <  SRR4302536.3984740/94‑1 (MQ=60)
                 CATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTT                                                                           <  SRR4302536.21263/94‑1 (MQ=60)
                  ATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTT                                                                          <  SRR4302536.6895526/94‑1 (MQ=60)
                  ATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTT                                                                          <  SRR4302536.4669003/94‑1 (MQ=60)
                  ATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTT                                                                          >  SRR4302536.2716544/1‑94 (MQ=60)
                   TCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTG                                                                         <  SRR4302536.3842850/94‑1 (MQ=60)
                   TCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTG                                                                         <  SRR4302536.4293534/94‑1 (MQ=60)
                   TCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTG                                                                         <  SRR4302536.3065523/94‑1 (MQ=60)
                    CCACGGCAATGTCTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGC                                                                        <  SRR4302536.666139/94‑1 (MQ=60)
                    CCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGC                                                                        <  SRR4302536.3193658/94‑1 (MQ=60)
                     CACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGATACCTTTTGCG                                                                       <  SRR4302536.1696794/94‑1 (MQ=60)
                     CACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCG                                                                       >  SRR4302536.6116383/1‑94 (MQ=60)
                     CACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCG                                                                       <  SRR4302536.1894993/94‑1 (MQ=60)
                     CACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCG                                                                       <  SRR4302536.47948/94‑1 (MQ=60)
                      CCGGCAATGACTTTCATATCGTATCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGC                                                                      <  SRR4302536.6225708/94‑1 (MQ=60)
                       CGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCC                                                                     <  SRR4302536.6607088/94‑1 (MQ=60)
                       CGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCC                                                                     >  SRR4302536.6053096/1‑94 (MQ=60)
                         GCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCAC                                                                   <  SRR4302536.6886475/94‑1 (MQ=60)
                          CAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACT                                                                  >  SRR4302536.3575530/1‑94 (MQ=60)
                          CAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACT                                                                  >  SRR4302536.27088/1‑94 (MQ=60)
                           AATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTG                                                                 >  SRR4302536.3679648/1‑94 (MQ=60)
                           AATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTG                                                                 >  SRR4302536.5740152/1‑94 (MQ=60)
                           AATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTG                                                                 >  SRR4302536.3121201/1‑94 (MQ=60)
                            ATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGG                                                                <  SRR4302536.1490855/94‑1 (MQ=60)
                            ATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGG                                                                >  SRR4302536.3539209/1‑94 (MQ=60)
                            ATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGG                                                                <  SRR4302536.3146734/94‑1 (MQ=60)
                            ATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGG                                                                >  SRR4302536.1247376/1‑94 (MQ=60)
                             TGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGC                                                               >  SRR4302536.1231179/1‑94 (MQ=60)
                              GACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCA                                                              <  SRR4302536.2434911/94‑1 (MQ=60)
                               ACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAG                                                             <  SRR4302536.2673848/94‑1 (MQ=60)
                                 TTTCATATCGTGGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTACGGAGCACAAATAACGAAACCTTTTGCGCCACTTGCAGCC                                                           >  SRR4302536.3182132/1‑94 (MQ=60)
                                 TTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCC                                                           >  SRR4302536.1474873/1‑94 (MQ=60)
                                  TTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCA                                                          <  SRR4302536.1010748/94‑1 (MQ=60)
                                  TTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCA                                                          <  SRR4302536.5211458/94‑1 (MQ=60)
                                    CATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGG                                                        >  SRR4302536.5952053/1‑94 (MQ=60)
                                    CATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGG                                                        >  SRR4302536.5431072/1‑94 (MQ=60)
                                    CATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGG                                                        >  SRR4302536.6142435/1‑94 (MQ=60)
                                    CATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGG                                                        >  SRR4302536.1545294/1‑94 (MQ=60)
                                      TATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCT                                                      <  SRR4302536.2270377/94‑1 (MQ=60)
                                      TATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCT                                                      <  SRR4302536.2244576/94‑1 (MQ=60)
                                      TATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCT                                                      <  SRR4302536.4200750/94‑1 (MQ=60)
                                       ATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTG                                                     <  SRR4302536.3609390/94‑1 (MQ=60)
                                         CGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTC                                                   <  SRR4302536.5707105/94‑1 (MQ=60)
                                           TAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGA                                                 >  SRR4302536.2745883/1‑94 (MQ=60)
                                            AGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAT                                                <  SRR4302536.6895342/94‑1 (MQ=60)
                                            AGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGAT                                                >  SRR4302536.5286475/1‑94 (MQ=60)
                                              CTACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCG                                              <  SRR4302536.6398344/94‑1 (MQ=60)
                                              CCACGCGCTTTCGCGACGATGGCAGAGCCGCGTTTGGGGTCCGGAGCACAAATAACGCAACCTTTTGCGCCACTGGCAGCCTGGCTGTCGATCG                                              >  SRR4302536.6231835/1‑94 (MQ=60)
                                              CCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCG                                              >  SRR4302536.4990544/1‑94 (MQ=60)
                                              CCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCG                                              <  SRR4302536.2252163/94‑1 (MQ=60)
                                                  GCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTT                                          <  SRR4302536.272478/94‑1 (MQ=60)
                                                   CGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTC                                         <  SRR4302536.3075789/94‑1 (MQ=60)
                                                    GCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCA                                        <  SRR4302536.4172360/94‑1 (MQ=60)
                                                    GCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCA                                        <  SRR4302536.6817878/94‑1 (MQ=60)
                                                    GCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCA                                        <  SRR4302536.6733774/94‑1 (MQ=60)
                                                     CTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAA                                       >  SRR4302536.3659300/1‑94 (MQ=60)
                                                     CTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAA                                       >  SRR4302536.1766618/1‑94 (MQ=60)
                                                       TTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATG                                     <  SRR4302536.1943993/94‑1 (MQ=60)
                                                       TTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATG                                     <  SRR4302536.1517296/94‑1 (MQ=60)
                                                         CGCGACGATGGCAGAGCCGAGTTTGGGTTCCGGAGCACAAATAACGAAACCTTTTGCCCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTT                                   <  SRR4302536.595553/94‑1 (MQ=60)
                                                         CGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTT                                   <  SRR4302536.245781/94‑1 (MQ=60)
                                                         CGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTT                                   <  SRR4302536.6911930/94‑1 (MQ=60)
                                                         CGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTT                                   <  SRR4302536.2728219/94‑1 (MQ=60)
                                                         CGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCACGCTGTCGATCGCGTTCAATGTT                                   <  SRR4302536.4365126/94‑1 (MQ=60)
                                                             ACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTT                               >  SRR4302536.719922/1‑94 (MQ=60)
                                                             ACGATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTT                               <  SRR4302536.2728081/94‑1 (MQ=60)
                                                               GATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCG                             <  SRR4302536.2225869/94‑1 (MQ=60)
                                                               GATGGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCG                             <  SRR4302536.3527938/94‑1 (MQ=60)
                                                                  GGCAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCA                          <  SRR4302536.6568006/94‑1 (MQ=60)
                                                                  GGCAGAGCCGAGTTTGGGGCCCGGAGCACAAATAACGAAACCTTTTGCGCCCCTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTGTTTAGCCA                          >  SRR4302536.810390/1‑94 (MQ=60)
                                                                    CAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATC                        >  SRR4302536.67652/1‑94 (MQ=60)
                                                                    CAGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATC                        >  SRR4302536.6374815/1‑94 (MQ=60)
                                                                     AGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCC                       >  SRR4302536.5942552/1‑94 (MQ=60)
                                                                     AGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCC                       <  SRR4302536.650985/94‑1 (MQ=60)
                                                                     AGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCC                       >  SRR4302536.2848049/1‑94 (MQ=60)
                                                                     AGAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCC                       >  SRR4302536.5358747/1‑94 (MQ=60)
                                                                      GAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCG                      >  SRR4302536.6806737/1‑94 (MQ=60)
                                                                      GAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCG                      <  SRR4302536.3708174/94‑1 (MQ=60)
                                                                      GAGCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCG                      <  SRR4302536.622740/94‑1 (MQ=60)
                                                                        GCCGAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGC                    >  SRR4302536.3030472/1‑94 (MQ=60)
                                                                           GAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACG                 >  SRR4302536.1653482/1‑94 (MQ=60)
                                                                           GAGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTAGCAGCCAGGCTGTCGATCCCGTTCAAGGTTTTTTTGCCATCCCGCACG                 >  SRR4302536.1727650/1‑94 (MQ=60)
                                                                            AGTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGCTCGCGATCAATGTTTTTTCGCCCTCCGGCACGG                >  SRR4302536.5774136/1‑94 (MQ=60)
                                                                             GTTTGGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGC               <  SRR4302536.3537221/94‑1 (MQ=60)
                                                                                TGGGGTCCGGAGCACAAAT‑ACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCCATGGTTTTTTGCCATCCGGCACGGCAATC           >  SRR4302536.6124910/1‑94 (MQ=60)
                                                                                 GGGGTCCGGAGCACAAATAACGAACCCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATC           <  SRR4302536.3851208/94‑1 (MQ=60)
                                                                                 GGGGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATC           >  SRR4302536.128704/1‑94 (MQ=60)
                                                                                   GGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTT         >  SRR4302536.640596/1‑94 (MQ=60)
                                                                                   GGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTT         <  SRR4302536.347368/94‑1 (MQ=60)
                                                                                   GGTCCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTT         >  SRR4302536.6474124/1‑94 (MQ=60)
                                                                                      CCGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAAT      >  SRR4302536.753223/1‑94 (MQ=60)
                                                                                       CGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATA     >  SRR4302536.6407104/1‑94 (MQ=60)
                                                                                       CGGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATA     >  SRR4302536.5240940/1‑94 (MQ=60)
                                                                                        GGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA    >  SRR4302536.6355305/1‑94 (MQ=60)
                                                                                        GGAGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAA    <  SRR4302536.4863884/94‑1 (MQ=60)
                                                                                          AGCACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAACC  >  SRR4302536.5454024/1‑94 (MQ=60)
                                                                                            |                                                                                           
GGCGTTAACAAACTGGTCATCCACGGCAATGACTTTCATATCGTAGCCACGCGCTTTCGCGACGATGGCAGAGCCGAGTTTGGGGTCCGGAGTACAAATAACGAAACCTTTTGCGCCACTGGCAGCCAGGCTGTCGATCGCGTTCAATGTTTTTTCGCCATCCGGCACGGCAATCTTAATAACC  >  NC_000913/1983798‑1983981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑