Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,063,343 T→C A148A (GCA→GCG)  cobU ← cobinamide kinase/cobinamide phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,063,3430TC99.5% 665.9 / NA 205A148A (GCA→GCG) cobUcobinamide kinase/cobinamide phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (114/90);  minor base A (0/1);  total (114/91)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATC  >  NC_000913/2063253‑2063430
                                                                                          |                                                                                       
tACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCgcgc                                                                                       >  1:6233129/1‑93 (MQ=255)
 aCTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGcc                                                                                      >  1:5534780/1‑93 (MQ=255)
 aCTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGcc                                                                                      >  2:2122581/1‑93 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                     >  1:1234352/1‑93 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                     >  1:5012522/1‑93 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                     >  1:6111267/1‑93 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                     <  1:2738388/93‑1 (MQ=255)
   tCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAg                                                                                    <  1:638058/93‑1 (MQ=255)
   tCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAg                                                                                    >  1:5681806/1‑93 (MQ=255)
    ccAATACCCGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                   <  1:5044948/93‑1 (MQ=255)
    ccAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                   >  2:3611757/1‑93 (MQ=255)
     cAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgac                                                                                  >  2:6156190/1‑93 (MQ=255)
      aaTACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                 >  1:4337477/1‑93 (MQ=255)
        tccccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                               <  1:4257917/91‑1 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCTACTGCTGAGTTACCCGCCCGGCAATAGCACGAAAATGTCGCGCCAgacgac                                                                               >  2:6722919/1‑93 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                               >  1:4823306/1‑93 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                              >  2:2370924/1‑93 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                              >  1:5399615/1‑93 (MQ=255)
           ccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActct                                                                            >  2:5764934/1‑93 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                           >  2:5833781/1‑93 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                           >  2:736665/1‑93 (MQ=255)
             gAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTcc                                                                          >  1:5182434/1‑93 (MQ=255)
             gAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTcc                                                                          >  1:3808315/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                         >  1:3570104/1‑93 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                      <  1:6159681/93‑1 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                      <  2:2995245/93‑1 (MQ=255)
                   accaGCCATACTTCATTTGCCGCAGCGGCGAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                    >  1:2239758/1‑93 (MQ=255)
                   accaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                    >  1:1328036/1‑93 (MQ=255)
                   accaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                    >  2:5254773/1‑93 (MQ=255)
                      aGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATc                                                                 <  2:3842103/93‑1 (MQ=255)
                      aGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATc                                                                 <  1:1902871/93‑1 (MQ=255)
                       gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                >  2:2160650/1‑93 (MQ=255)
                       gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                >  1:2727569/1‑93 (MQ=255)
                         cATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcccc                                                              >  2:4621837/1‑93 (MQ=255)
                         cATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcccc                                                              <  2:83962/93‑1 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                             <  2:6306279/93‑1 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                             <  2:3072982/93‑1 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                            >  1:1280024/1‑93 (MQ=255)
                            aCTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAtt                                                           >  1:5908225/1‑93 (MQ=255)
                            aCTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAtt                                                           >  1:2121725/1‑93 (MQ=255)
                              ttCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTcc                                                         >  1:1119465/1‑93 (MQ=255)
                              ttCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTcc                                                         >  2:5085098/1‑93 (MQ=255)
                              ttCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTcc                                                         >  2:4049241/1‑93 (MQ=255)
                               tCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTccc                                                        >  2:1527925/1‑93 (MQ=255)
                                 aTTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAc                                                      >  1:1356926/1‑93 (MQ=255)
                                 aTTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAc                                                      <  2:4323837/93‑1 (MQ=255)
                                   ttGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACtt                                                    <  2:707040/93‑1 (MQ=255)
                                     gCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                  >  1:4486001/1‑93 (MQ=255)
                                        ccAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTa                                               <  1:4556949/92‑1 (MQ=255)
                                         cAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAg                                              <  1:4962581/93‑1 (MQ=255)
                                         cAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAg                                              <  1:2713196/93‑1 (MQ=255)
                                         cAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAg                                              >  1:4122291/1‑93 (MQ=255)
                                           gCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTc                                            <  1:366992/93‑1 (MQ=255)
                                            cGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCa                                           <  2:4983822/93‑1 (MQ=255)
                                             ggCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAc                                          <  2:318578/93‑1 (MQ=255)
                                             ggCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAc                                          >  1:1638711/1‑93 (MQ=255)
                                             ggCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAc                                          <  2:5588518/93‑1 (MQ=255)
                                             cgCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAc                                          <  1:5507823/92‑1 (MQ=255)
                                                cAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTaa                                       <  2:3411270/93‑1 (MQ=255)
                                                cAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTaa                                       <  1:1372458/93‑1 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                     >  2:1398066/1‑93 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                     <  1:2032620/93‑1 (MQ=255)
                                                   ctgctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAc                                    <  1:99658/93‑1 (MQ=255)
                                                    tgctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACa                                   <  1:1473217/93‑1 (MQ=255)
                                                     gctgATTTACCCGCCCGGCAATTTCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACac                                  >  1:472328/1‑92 (MQ=255)
                                                     gctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACaa                                  >  1:4815927/1‑93 (MQ=255)
                                                       tgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                >  2:4142303/1‑93 (MQ=255)
                                                        gATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCt                               >  2:6090398/1‑93 (MQ=255)
                                                        gATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCt                               >  2:4577955/1‑93 (MQ=255)
                                                        gATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCt                               >  1:4925339/1‑93 (MQ=255)
                                                        gATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCt                               >  2:4168308/1‑93 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                              <  2:1950787/93‑1 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                              <  2:1764324/93‑1 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                              >  2:2447100/1‑93 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                              <  1:5368420/93‑1 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCAGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                              >  2:5850358/1‑93 (MQ=255)
                                                                 gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                      >  2:271552/1‑93 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCggggga                     >  1:4110406/1‑91 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                     >  2:4503601/1‑93 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                     <  2:4820054/93‑1 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                     >  1:3518517/1‑93 (MQ=255)
                                                                   ccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCaa                    <  1:6636680/93‑1 (MQ=255)
                                                                    cgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAc                   >  1:2599288/1‑93 (MQ=255)
                                                                     ggCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACg                  >  2:6062446/1‑93 (MQ=255)
                                                                      gCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCGCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGt                 <  1:3844901/93‑1 (MQ=255)
                                                                      gCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGt                 >  1:2100444/1‑93 (MQ=255)
                                                                       cAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGtt                >  2:491202/1‑93 (MQ=255)
                                                                        aaTATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTg               <  2:2876415/93‑1 (MQ=255)
                                                                        aaTATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTg               >  1:1776506/1‑93 (MQ=255)
                                                                         atatCACGAAAATGTCGCGCCAGACGGCTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTgg              <  1:2912509/93‑1 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc             <  2:5456556/93‑1 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc             <  2:5463284/93‑1 (MQ=255)
                                                                           atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggca            >  1:733630/1‑93 (MQ=255)
                                                                            tCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcca           >  2:6432729/1‑91 (MQ=255)
                                                                             cACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcagg          <  2:6648873/93‑1 (MQ=255)
                                                                                gAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGgcaggcag       <  2:402258/93‑1 (MQ=255)
                                                                                  aaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagca     <  1:3016917/93‑1 (MQ=255)
                                                                                   aaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCaa    >  2:112698/1‑93 (MQ=255)
                                                                                    aTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAAt   <  1:3958998/93‑1 (MQ=255)
                                                                                     tGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATc  <  2:3320190/93‑1 (MQ=255)
                                                                                          |                                                                                       
TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATC  >  NC_000913/2063253‑2063430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  >  NC_000913/2063251‑2063436
                                                                                            |                                                                                             
TTTACTCCAATACCGGAACCCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCG                                                                                              <  SRR4302536.5212204/94‑1 (MQ=60)
TTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCG                                                                                              >  SRR4302536.336106/1‑94 (MQ=60)
 TTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGC                                                                                             >  SRR4302536.6418834/1‑94 (MQ=60)
  TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCC                                                                                            >  SRR4302536.5699750/1‑94 (MQ=60)
   ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                           >  SRR4302536.1271414/1‑94 (MQ=60)
   ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                           >  SRR4302536.5162849/1‑94 (MQ=60)
   ACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCA                                                                                           >  SRR4302536.6293435/1‑94 (MQ=60)
    CTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAG                                                                                          <  SRR4302536.2819979/94‑1 (MQ=60)
    CTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAG                                                                                          >  SRR4302536.5850744/1‑94 (MQ=60)
     TCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCACTATCACGAAAATGTCGCGCCAGA                                                                                         >  SRR4302536.3666787/1‑94 (MQ=60)
     TCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGA                                                                                         <  SRR4302536.656901/94‑1 (MQ=60)
      CCAATACCCGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGAC                                                                                        <  SRR4302536.5196132/94‑1 (MQ=60)
       CAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                       >  SRR4302536.4467397/1‑94 (MQ=60)
       CAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACG                                                                                       >  SRR4302536.4365259/1‑94 (MQ=60)
         ATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAC                                                                                     >  SRR4302536.4968460/1‑94 (MQ=60)
          TCCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACT                                                                                    <  SRR4302536.4384772/94‑1 (MQ=60)
          TACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACT                                                                                    >  SRR4302536.5560902/1‑94 (MQ=60)
          TACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACT                                                                                    >  SRR4302536.4443289/1‑94 (MQ=60)
           ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   >  SRR4302536.2801994/1‑94 (MQ=60)
           ACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTC                                                                                   >  SRR4302536.4780245/1‑94 (MQ=60)
            CCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCT                                                                                  >  SRR4302536.5564648/1‑94 (MQ=60)
              CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                                >  SRR4302536.5337014/1‑94 (MQ=60)
              CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                                <  SRR4302536.1374428/94‑1 (MQ=60)
              CGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCC                                                                                >  SRR4302536.3921836/1‑94 (MQ=60)
               GAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCG                                                                               >  SRR4302536.3676841/1‑94 (MQ=60)
                 AACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGC                                                                             >  SRR4302536.6313729/1‑94 (MQ=60)
                 AACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGC                                                                             <  SRR4302536.2164869/94‑1 (MQ=60)
                 AACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGC                                                                             >  SRR4302536.131134/1‑94 (MQ=60)
                   CCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCAC                                                                           <  SRR4302536.6343177/94‑1 (MQ=60)
                    CACCAGCCATACTTCATTTGCCGCAGCGGCGAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACA                                                                          >  SRR4302536.2307525/1‑94 (MQ=60)
                    CACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACA                                                                          <  SRR4302536.36148/94‑1 (MQ=60)
                    CACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACA                                                                          >  SRR4302536.1367673/1‑94 (MQ=60)
                     ACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAA                                                                         >  SRR4302536.951973/1‑94 (MQ=60)
                        AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                      >  SRR4302536.2808885/1‑94 (MQ=60)
                        AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                      <  SRR4302536.1960163/94‑1 (MQ=60)
                        AGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATAACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCC                                                                      >  SRR4302536.5335476/1‑94 (MQ=60)
                          CCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCC                                                                    >  SRR4302536.6558987/1‑94 (MQ=60)
                            ATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAT                                                                  >  SRR4302536.1318335/1‑94 (MQ=60)
                             TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 >  SRR4302536.2185479/1‑94 (MQ=60)
                             TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 <  SRR4302536.2818894/94‑1 (MQ=60)
                             TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 <  SRR4302536.5717616/94‑1 (MQ=60)
                             TACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATT                                                                 >  SRR4302536.6084193/1‑94 (MQ=60)
                              ACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTC                                                                <  SRR4302536.2283779/94‑1 (MQ=60)
                               CTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCC                                                               >  SRR4302536.4557577/1‑94 (MQ=60)
                               CTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCC                                                               >  SRR4302536.1153077/1‑94 (MQ=60)
                                  CATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCAC                                                            >  SRR4302536.1397365/1‑94 (MQ=60)
                                    TTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTT                                                          >  SRR4302536.1334768/1‑94 (MQ=60)
                                    TTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTT                                                          <  SRR4302536.3924441/94‑1 (MQ=60)
                                      TGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCG                                                        >  SRR4302536.4620729/1‑94 (MQ=60)
                                       GCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGT                                                       >  SRR4302536.5696599/1‑94 (MQ=60)
                                       GCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGT                                                       >  SRR4302536.5986138/1‑94 (MQ=60)
                                         CGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTA                                                     <  SRR4302536.5098819/94‑1 (MQ=60)
                                          GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                    <  SRR4302536.1612743/94‑1 (MQ=60)
                                          GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                    <  SRR4302536.2079536/94‑1 (MQ=60)
                                          GCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                    >  SRR4302536.4244511/1‑94 (MQ=60)
                                          CCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAG                                                    <  SRR4302536.4693645/94‑1 (MQ=60)
                                           CAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGT                                                   <  SRR4302536.5111668/94‑1 (MQ=60)
                                           CAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGT                                                   <  SRR4302536.1474757/94‑1 (MQ=60)
                                           CAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGT                                                   <  SRR4302536.2794132/94‑1 (MQ=60)
                                            AGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTC                                                  >  SRR4302536.2025141/1‑94 (MQ=60)
                                            AGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCACATTCCCACTTCGTTAGTC                                                  >  SRR4302536.3413498/1‑94 (MQ=60)
                                             GCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCA                                                 <  SRR4302536.376692/94‑1 (MQ=60)
                                              CGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCAC                                                >  SRR4302536.1686416/1‑94 (MQ=60)
                                               CGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACT                                               <  SRR4302536.5672128/94‑1 (MQ=60)
                                                 CCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAA                                             <  SRR4302536.5531455/94‑1 (MQ=60)
                                                  CAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAT                                            <  SRR4302536.1413354/94‑1 (MQ=60)
                                                   AACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATA                                           >  SRR4302536.6585676/1‑94 (MQ=60)
                                                    ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                          <  SRR4302536.2093829/94‑1 (MQ=60)
                                                    ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                          <  SRR4302536.6103656/94‑1 (MQ=60)
                                                    ACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCACCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAC                                          <  SRR4302536.3046911/94‑1 (MQ=60)
                                                     CTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACA                                         >  SRR4302536.6389191/1‑94 (MQ=60)
                                                     CTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACA                                         <  SRR4302536.102318/94‑1 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATTTCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAC                                        >  SRR4302536.484647/1‑94 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        <  SRR4302536.1516778/94‑1 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        <  SRR4302536.1162949/94‑1 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        >  SRR4302536.4237605/1‑94 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        >  SRR4302536.4960861/1‑94 (MQ=60)
                                                      TGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAA                                        >  SRR4302536.4960860/1‑94 (MQ=60)
                                                         TGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     >  SRR4302536.5073336/1‑94 (MQ=60)
                                                         TGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     <  SRR4302536.4054568/94‑1 (MQ=60)
                                                         TGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     <  SRR4302536.374046/94‑1 (MQ=60)
                                                         TGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCT                                     <  SRR4302536.376869/94‑1 (MQ=60)
                                                          GATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTT                                    >  SRR4302536.4129726/1‑94 (MQ=60)
                                                          GATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTT                                    >  SRR4302536.2501821/1‑94 (MQ=60)
                                                          GATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTT                                    >  SRR4302536.2149319/1‑94 (MQ=60)
                                                           ATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTT                                   <  SRR4302536.5528539/94‑1 (MQ=60)
                                                               ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGG                               >  SRR4302536.1946080/1‑94 (MQ=60)
                                                               ACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGG                               >  SRR4302536.2739720/1‑94 (MQ=60)
                                                                   GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGGA                           >  SRR4302536.4232344/1‑94 (MQ=60)
                                                                   GCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCA                           >  SRR4302536.3623621/1‑94 (MQ=60)
                                                                    CCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAA                          >  SRR4302536.53943/1‑94 (MQ=60)
                                                                     CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                         >  SRR4302536.2677349/1‑94 (MQ=60)
                                                                     CCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAAC                         <  SRR4302536.6834049/94‑1 (MQ=60)
                                                                      CGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACG                        >  SRR4302536.4635119/1‑94 (MQ=60)
                                                                       GGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGT                       >  SRR4302536.2163610/1‑94 (MQ=60)
                                                                        GCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCGCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTC                      <  SRR4302536.3959375/94‑1 (MQ=60)
                                                                        GCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTT                      <  SRR4302536.5002719/94‑1 (MQ=60)
                                                                         CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTG                     >  SRR4302536.1830249/1‑94 (MQ=60)
                                                                         CAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGCTAGTCACTAATACAACCTCTGCGTGGCAACGTTG                     >  SRR4302536.5148391/1‑94 (MQ=60)
                                                                           ATATCACGAAAATGTCGCGCCAGACGGCTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGC                   <  SRR4302536.2998496/94‑1 (MQ=60)
                                                                            TATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCA                  <  SRR4302536.4568972/94‑1 (MQ=60)
                                                                            TATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCA                  >  SRR4302536.755145/1‑94 (MQ=60)
                                                                               CACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGC               >  SRR4302536.5168909/1‑94 (MQ=60)
                                                                                 CGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATAGAACCTTTGCGGGGCAACGTTGGCAGGCAG             >  SRR4302536.6795967/1‑94 (MQ=60)
                                                                                    AAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAA          <  SRR4302536.3105576/94‑1 (MQ=60)
                                                                                      ATGTCGCGCCAGACGCCTCTCCGACACAATCCCCATTCCCGCTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATC        <  SRR4302536.2887327/94‑1 (MQ=60)
                                                                                      ATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATC        <  SRR4302536.4076561/94‑1 (MQ=60)
                                                                                       TGTCGCGCCAGACGCCTCTCCGGCACAATCCCCATTCCCGCTTCGTTAGTCACTAAGACAGCCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302536.1727126/94‑1 (MQ=60)
                                                                                       TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302536.1413179/94‑1 (MQ=60)
                                                                                       TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302536.5458169/94‑1 (MQ=60)
                                                                                       TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302536.692590/94‑1 (MQ=60)
                                                                                       TGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCA       <  SRR4302536.4922667/94‑1 (MQ=60)
                                                                                         TCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACACCCTTTGCGGGGCACCGTTGGCAGGCAGCAATCAAC     <  SRR4302536.2272104/94‑1 (MQ=60)
                                                                                         TCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAAC     >  SRR4302536.636809/1‑94 (MQ=60)
                                                                                          CGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACG    <  SRR4302536.4068881/94‑1 (MQ=60)
                                                                                            CGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  <  SRR4302536.3054646/94‑1 (MQ=60)
                                                                                            |                                                                                             
TTTACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAACGAC  >  NC_000913/2063251‑2063436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑