Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,074,245 T→C M484T (ATG→ACG)  b2001 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,2450TC100.0% 751.4 / NA 233M484T (ATG→ACG) b2001hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (100/133);  total (100/133)

Only 100 of 243 total aligned reads displayed.

GAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAC  >  NC_000913/2074156‑2074334
                                                                                         |                                                                                         
gAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCa                                                                                        <  2:2912156/93‑1 (MQ=255)
gAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGAGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCa                                                                                        >  2:3946671/1‑93 (MQ=255)
 aaaTATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCaa                                                                                       >  1:1057636/1‑93 (MQ=255)
  aaTATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAAc                                                                                      >  2:6544519/1‑93 (MQ=255)
  aaTATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAAc                                                                                      >  1:3929525/1‑93 (MQ=255)
   atatTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACt                                                                                     <  2:3770924/93‑1 (MQ=255)
   atatTGTACAACTGCAGCACGAAGCGTCGGTGCCGCATTCGACAGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACt                                                                                     <  1:4476279/93‑1 (MQ=255)
    tatTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTg                                                                                    >  2:2408941/1‑93 (MQ=255)
    tatTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTg                                                                                    >  1:4489910/1‑93 (MQ=255)
    tatTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGACCCACGCAACTg                                                                                    >  1:1714385/1‑93 (MQ=255)
     atTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGCAATAATGACCCCACGAAACTgg                                                                                   >  2:547037/1‑93 (MQ=255)
     atTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTgg                                                                                   >  1:30601/1‑93 (MQ=255)
      ttGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGa                                                                                  >  1:5088570/1‑93 (MQ=255)
        gTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAg                                                                                <  1:5151018/93‑1 (MQ=255)
         ttCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGt                                                                               >  1:5663168/1‑93 (MQ=255)
            aaCTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTccc                                                                            <  2:3658443/93‑1 (MQ=255)
             aCTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTcccc                                                                           <  1:6380666/93‑1 (MQ=255)
               tGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCg                                                                         <  1:4961631/93‑1 (MQ=255)
               tGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCg                                                                         >  1:4294663/1‑93 (MQ=255)
                gCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCgg                                                                        >  1:527362/1‑92 (MQ=255)
                 cAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTg                                                                       <  2:4379717/93‑1 (MQ=255)
                    cccGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATc                                                                    <  1:2206003/93‑1 (MQ=255)
                       gTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGt                                                                 >  2:2383308/1‑93 (MQ=255)
                       gTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGt                                                                 >  1:864032/1‑93 (MQ=255)
                         aGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGTCCCACGCAACTGGAAGTTCCCCGTGGTCAGTAt                                                               >  1:773689/1‑93 (MQ=255)
                         aGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTAt                                                               <  2:6629443/93‑1 (MQ=255)
                          gCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATg                                                              >  1:2224227/1‑93 (MQ=255)
                          gCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATg                                                              >  2:4242146/1‑93 (MQ=255)
                            gTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGcc                                                            >  1:3439424/1‑93 (MQ=255)
                             tCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCCGTATGCCg                                                           >  2:6849/1‑93 (MQ=255)
                             tCGGGGCGGCATTCGACGGACAAAACGGACAGTATCGGTACATGGGAAATAATGGCCCCACGCCACTGGAAGTCCCCCGAGATCAGTATGACg                                                           >  1:4327224/1‑93 (MQ=255)
                               ggTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGgc                                                         <  1:4220851/93‑1 (MQ=255)
                               ggTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGgc                                                         <  2:5868005/93‑1 (MQ=255)
                                gTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGgcg                                                        >  1:4001583/1‑93 (MQ=255)
                                 tGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGgcgc                                                       <  1:6711486/93‑1 (MQ=255)
                                  gCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCt                                                      >  1:3212860/1‑93 (MQ=255)
                                   cGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTg                                                     <  2:100101/93‑1 (MQ=255)
                                    ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGt                                                    >  2:2650864/1‑93 (MQ=255)
                                    ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGt                                                    >  2:2309633/1‑93 (MQ=255)
                                    ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGt                                                    >  2:2092264/1‑93 (MQ=255)
                                    ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGt                                                    <  1:4224274/93‑1 (MQ=255)
                                     gCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGtt                                                   >  2:3745946/1‑93 (MQ=255)
                                     gCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGtt                                                   <  1:3010900/93‑1 (MQ=255)
                                       aTTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGa                                                 <  2:3598847/93‑1 (MQ=255)
                                       aTTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGa                                                 <  2:6016389/93‑1 (MQ=255)
                                        ttCGACGGACAAAACGGACAGTATCGCTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGaa                                                >  2:5401971/1‑93 (MQ=255)
                                         tCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGaaa                                               >  1:2292790/1‑93 (MQ=255)
                                         tCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGaaa                                               >  2:6405327/1‑93 (MQ=255)
                                          cGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAAc                                              <  2:6505723/93‑1 (MQ=255)
                                          cGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAAc                                              >  1:4537428/1‑93 (MQ=255)
                                            aCGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCa                                            <  2:1395524/93‑1 (MQ=255)
                                             cGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCAt                                           <  1:3512194/93‑1 (MQ=255)
                                             cGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCAt                                           <  1:16515/93‑1 (MQ=255)
                                               gACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGa                                         >  1:6129666/1‑93 (MQ=255)
                                                 cAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAgg                                       <  2:3400644/93‑1 (MQ=255)
                                                   aaaCGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGaa                                     <  2:2981613/93‑1 (MQ=255)
                                                   aaaCGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGaa                                     >  2:1675265/1‑93 (MQ=255)
                                                   aaaCGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACAATGAGGaa                                     >  2:1222733/1‑93 (MQ=255)
                                                     aCGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATa                                   <  2:3969944/93‑1 (MQ=255)
                                                      cGGACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATCCCGCGGCTGTTGAAACAATGAGGAATaa                                  >  1:4262740/1‑93 (MQ=255)
                                                      cGGACAGTATCGTTACATGGGAAATAATGGCCCAACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATaa                                  <  2:4658653/93‑1 (MQ=255)
                                                       ggACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAg                                 <  2:1371124/93‑1 (MQ=255)
                                                       ggACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAg                                 <  1:5742905/93‑1 (MQ=255)
                                                       ggACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAg                                 <  1:5049175/93‑1 (MQ=255)
                                                       ggACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAg                                 <  2:4792827/93‑1 (MQ=255)
                                                        gACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGa                                >  2:1519812/1‑93 (MQ=255)
                                                        gACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGa                                <  1:2776028/93‑1 (MQ=255)
                                                        gACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGa                                >  1:3298333/1‑93 (MQ=255)
                                                        gACAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGCCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGa                                <  1:585603/93‑1 (MQ=255)
                                                         aCAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGAt                               <  1:2081590/93‑1 (MQ=255)
                                                         aCAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGAt                               <  1:3750434/93‑1 (MQ=255)
                                                          cAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATc                              <  1:4778329/93‑1 (MQ=255)
                                                          cAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATc                              >  2:5674957/1‑93 (MQ=255)
                                                          cAGTATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATc                              >  1:2543626/1‑93 (MQ=255)
                                                             tATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCcgc                           <  1:1026619/93‑1 (MQ=255)
                                                             tATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCcgc                           >  2:1890963/1‑93 (MQ=255)
                                                             tATCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCcgc                           >  2:4101926/1‑93 (MQ=255)
                                                               tCGTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGa                         <  2:1559946/93‑1 (MQ=255)
                                                                cGTTACATGGGAAATAATGTCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGaa                        <  2:2624934/93‑1 (MQ=255)
                                                                 gTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAg                       <  2:6204824/93‑1 (MQ=255)
                                                                 gTTACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAg                       >  1:1097269/1‑93 (MQ=255)
                                                                   tACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGt                     <  1:5188535/93‑1 (MQ=255)
                                                                   tACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGt                     <  1:5523530/93‑1 (MQ=255)
                                                                   tACATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGt                     >  2:7146/1‑93 (MQ=255)
                                                                    aCATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGACGAATAAGATCCGCGAAGGTa                    >  1:6076100/1‑93 (MQ=255)
                                                                     cATGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTaa                   <  1:1480834/93‑1 (MQ=255)
                                                                      aTGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTaaa                  <  2:528189/93‑1 (MQ=255)
                                                                       tGGGAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAg                 <  2:4520484/93‑1 (MQ=255)
                                                                          gAAATAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAAACATCAGGAATAAGATCCGCGAAGGTAAAGTaa              >  2:3362111/1‑92 (MQ=255)
                                                                           aaaTAATGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTaga             <  2:912107/93‑1 (MQ=255)
                                                                            aataatGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTagag            <  1:2620952/93‑1 (MQ=255)
                                                                               aatGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAgaggag         <  2:1817532/93‑1 (MQ=255)
                                                                                atGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGgaga        >  2:1771321/1‑93 (MQ=255)
                                                                                atGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGgaga        <  2:1262851/93‑1 (MQ=255)
                                                                                atGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGgaga        >  1:6048742/1‑93 (MQ=255)
                                                                                 tGGCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCGGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGgagag       >  2:3084175/1‑93 (MQ=255)
                                                                                   gCCCCACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGAt     <  2:5108025/93‑1 (MQ=255)
                                                                                    gcccACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATg    <  1:220668/92‑1 (MQ=255)
                                                                                     cccACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGa   <  1:2876407/93‑1 (MQ=255)
                                                                                      ccACGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAc  <  1:6326467/93‑1 (MQ=255)
                                                                                         |                                                                                         
GAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAC  >  NC_000913/2074156‑2074334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAT‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCC  >  NC_000913/2074152‑2074337
                                                                                             |                                                                                             
AGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC                                                                                               >  SRR4302536.2906726/1‑94 (MQ=60)
AGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC                                                                                               >  SRR4302536.1824649/1‑94 (MQ=60)
 GACGAAATATGGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑G                                                                                             <  SRR4302536.1548456/94‑1 (MQ=60)
   CGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCA                                                                                           >  SRR4302536.5941688/1‑94 (MQ=60)
    GAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAA                                                                                          >  SRR4302536.1089259/1‑94 (MQ=60)
     AAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAAC                                                                                         >  SRR4302536.4046279/1‑94 (MQ=60)
      AATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACT                                                                                        >  SRR4302536.4074573/1‑94 (MQ=60)
       ATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTG                                                                                       >  SRR4302536.4624723/1‑94 (MQ=60)
       ATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGACCCAC‑GCAACTG                                                                                       >  SRR4302536.1763956/1‑94 (MQ=60)
       ATATTGTACAACTGCAGCACGAAGCGTCGGTGCCGCATTCGACAGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTG                                                                                       <  SRR4302536.4610754/94‑1 (MQ=60)
        TATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGG                                                                                      >  SRR4302536.31443/1‑94 (MQ=60)
         ATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGA                                                                                     >  SRR4302536.5240778/1‑94 (MQ=60)
         ATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGA                                                                                     >  SRR4302536.5213927/1‑94 (MQ=60)
            GTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGT                                                                                  >  SRR4302536.5831481/1‑94 (MQ=60)
            GTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGT                                                                                  <  SRR4302536.5304702/94‑1 (MQ=60)
               CAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCC                                                                               >  SRR4302536.3393583/1‑94 (MQ=60)
               CAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCC                                                                               >  SRR4302536.5188097/1‑94 (MQ=60)
                 ACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCC                                                                             <  SRR4302536.6570879/94‑1 (MQ=60)
                  CTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCG                                                                            <  SRR4302536.538523/94‑1 (MQ=60)
                  CTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCG                                                                            >  SRR4302536.4423010/1‑94 (MQ=60)
                   TGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGT                                                                           <  SRR4302536.5110688/94‑1 (MQ=60)
                   TGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGG                                                                           >  SRR4302536.540850/1‑94 (MQ=60)
                   TGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCACCCGT                                                                           >  SRR4302536.3456128/1‑94 (MQ=60)
                     CAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGA                                                                         >  SRR4302536.2695077/1‑94 (MQ=60)
                      AGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGAT                                                                        >  SRR4302536.4380919/1‑94 (MQ=60)
                       GCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATC                                                                       >  SRR4302536.5272620/1‑94 (MQ=60)
                        CCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCA                                                                      <  SRR4302536.2272609/94‑1 (MQ=60)
                        CCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCA                                                                      <  SRR4302536.304539/94‑1 (MQ=60)
                        CCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCA                                                                      <  SRR4302536.1121876/94‑1 (MQ=60)
                         CCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAG                                                                     >  SRR4302536.2676690/1‑94 (MQ=60)
                         CCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTAAATGGGCAATAATGGAGCCAC‑GCCACTGGAAGTAACCCGTGATCAA                                                                     >  SRR4302536.1988671/1‑94 (MQ=60)
                          CGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGT                                                                    >  SRR4302536.4259429/1‑94 (MQ=60)
                          CGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGT                                                                    >  SRR4302536.2546980/1‑94 (MQ=60)
                          CGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGT                                                                    >  SRR4302536.889437/1‑94 (MQ=60)
                           GTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTA                                                                   >  SRR4302536.218446/1‑94 (MQ=60)
                            TAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGTCCCAC‑GCAACTGGAAGTTCCCCGTGGTCAGTAT                                                                  >  SRR4302536.796365/1‑94 (MQ=60)
                            TAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTAT                                                                  >  SRR4302536.6031138/1‑94 (MQ=60)
                             AGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATG                                                                 >  SRR4302536.2291503/1‑94 (MQ=60)
                              GCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGC                                                                <  SRR4302536.5234980/94‑1 (MQ=60)
                               CGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCC                                                               >  SRR4302536.3541852/1‑94 (MQ=60)
                                GTCGGGGCGGCATTCGACGGACAAAACGGACAGTATCGGTACATGGGAAATAATGGCCCCAC‑GCCACTGGAAGTCCCCCGAGATCAGTATGACG                                                              >  SRR4302536.4456798/1‑94 (MQ=60)
                                 TCGGTGCGTCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGG                                                             <  SRR4302536.3448387/94‑1 (MQ=60)
                                 TCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGG                                                             <  SRR4302536.4439495/94‑1 (MQ=60)
                                  CGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGC                                                            <  SRR4302536.750202/94‑1 (MQ=60)
                                   GGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCG                                                           >  SRR4302536.4120314/1‑94 (MQ=60)
                                   GGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCG                                                           <  SRR4302536.4708013/94‑1 (MQ=60)
                                   GGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCG                                                           <  SRR4302536.4346423/94‑1 (MQ=60)
                                    GTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGC                                                          <  SRR4302536.4386654/94‑1 (MQ=60)
                                     TGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCT                                                         <  SRR4302536.4555781/94‑1 (MQ=60)
                                     TGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCT                                                         >  SRR4302536.3307099/1‑94 (MQ=60)
                                     TGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCT                                                         <  SRR4302536.6910743/94‑1 (MQ=60)
                                      GCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTG                                                        <  SRR4302536.385370/94‑1 (MQ=60)
                                        GGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTT                                                      <  SRR4302536.4349958/94‑1 (MQ=60)
                                        GGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTT                                                      <  SRR4302536.3106668/94‑1 (MQ=60)
                                         GCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTG                                                     <  SRR4302536.3099400/94‑1 (MQ=60)
                                          CATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATTGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGA                                                    >  SRR4302536.5810903/1‑94 (MQ=60)
                                          CATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGA                                                    >  SRR4302536.2338601/1‑94 (MQ=60)
                                           ATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAA                                                   >  SRR4302536.2241607/1‑94 (MQ=60)
                                           ATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAA                                                   >  SRR4302536.6356096/1‑94 (MQ=60)
                                            TTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAA                                                  >  SRR4302536.1644794/1‑94 (MQ=60)
                                            TTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAA                                                  >  SRR4302536.2362340/1‑94 (MQ=60)
                                             TCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAAC                                                 >  SRR4302536.4673482/1‑94 (MQ=60)
                                              CGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACC                                                <  SRR4302536.844486/94‑1 (MQ=60)
                                               GACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCA                                               >  SRR4302536.5397464/1‑94 (MQ=60)
                                                ACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCAT                                              <  SRR4302536.4207083/94‑1 (MQ=60)
                                                 CGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATG                                             <  SRR4302536.3617062/94‑1 (MQ=60)
                                                 CGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATG                                             <  SRR4302536.16978/94‑1 (MQ=60)
                                                  GGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGA                                            >  SRR4302536.6312348/1‑94 (MQ=60)
                                                    ACAAAACGGAGAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGG                                          <  SRR4302536.2906664/94‑1 (MQ=60)
                                                    ACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGG                                          <  SRR4302536.113122/94‑1 (MQ=60)
                                                    ACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGG                                          >  SRR4302536.459528/1‑94 (MQ=60)
                                                     CAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGA                                         >  SRR4302536.1739164/1‑94 (MQ=60)
                                                      AAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAA                                        <  SRR4302536.228983/94‑1 (MQ=60)
                                                        AACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATA                                      <  SRR4302536.3674715/94‑1 (MQ=60)
                                                        AACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATA                                      <  SRR4302536.6523138/94‑1 (MQ=60)
                                                         ACGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATCCCGCGGCTGTTGAAACAATGAGGAATAA                                     >  SRR4302536.4389789/1‑94 (MQ=60)
                                                          CGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAG                                    >  SRR4302536.6237506/1‑94 (MQ=60)
                                                          CGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAG                                    <  SRR4302536.6680952/94‑1 (MQ=60)
                                                          CGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAG                                    <  SRR4302536.4652116/94‑1 (MQ=60)
                                                          CGGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAG                                    >  SRR4302536.1830933/1‑94 (MQ=60)
                                                           GGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGA                                   <  SRR4302536.5913740/94‑1 (MQ=60)
                                                           GGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGA                                   >  SRR4302536.3395527/1‑94 (MQ=60)
                                                           GGACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGA                                   <  SRR4302536.5200475/94‑1 (MQ=60)
                                                            GACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGAT                                  <  SRR4302536.2858508/94‑1 (MQ=60)
                                                            GACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGCCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGAT                                  <  SRR4302536.600608/94‑1 (MQ=60)
                                                             ACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATC                                 <  SRR4302536.3862526/94‑1 (MQ=60)
                                                             ACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATC                                 <  SRR4302536.2144255/94‑1 (MQ=60)
                                                             ACAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATC                                 >  SRR4302536.2620246/1‑94 (MQ=60)
                                                              CAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCC                                <  SRR4302536.4922202/94‑1 (MQ=60)
                                                              CAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCC                                >  SRR4302536.1751059/1‑94 (MQ=60)
                                                              CAGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCC                                <  SRR4302536.2919881/94‑1 (MQ=60)
                                                               AGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCG                               >  SRR4302536.76624/1‑94 (MQ=60)
                                                               AGTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCG                               <  SRR4302536.6307723/94‑1 (MQ=60)
                                                                GTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGC                              >  SRR4302536.4668146/1‑94 (MQ=60)
                                                                GTATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGC                              <  SRR4302536.4456057/94‑1 (MQ=60)
                                                                 TATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCG                             >  SRR4302536.2763558/1‑94 (MQ=60)
                                                                 TATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCG                             <  SRR4302536.1057032/94‑1 (MQ=60)
                                                                  ATCGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGA                            >  SRR4302536.5503672/1‑94 (MQ=60)
                                                                    CGTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAG                          >  SRR4302536.1130179/1‑94 (MQ=60)
                                                                     GTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGG                         <  SRR4302536.5901638/94‑1 (MQ=60)
                                                                     GTTACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGG                         <  SRR4302536.6538621/94‑1 (MQ=60)
                                                                       TACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTA                       >  SRR4302536.912876/1‑94 (MQ=60)
                                                                       TACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTA                       <  SRR4302536.5688231/94‑1 (MQ=60)
                                                                       TACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTA                       <  SRR4302536.5343282/94‑1 (MQ=60)
                                                                       TACATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGACGAATAAGATCCGCGAAGGTA                       >  SRR4302536.6257347/1‑94 (MQ=60)
                                                                         CATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAA                     <  SRR4302536.1524593/94‑1 (MQ=60)
                                                                          ATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAG                    <  SRR4302536.5932189/94‑1 (MQ=60)
                                                                          ATGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAG                    <  SRR4302536.5094707/94‑1 (MQ=60)
                                                                           TGGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGT                   <  SRR4302536.2273070/94‑1 (MQ=60)
                                                                            GGGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTA                  <  SRR4302536.5067529/94‑1 (MQ=60)
                                                                             GGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAG                 <  SRR4302536.5766584/94‑1 (MQ=60)
                                                                             GGAAATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAG                 >  SRR4302536.4403532/1‑94 (MQ=60)
                                                                                AATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGG              <  SRR4302536.2699572/94‑1 (MQ=60)
                                                                                 ATAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGA             <  SRR4302536.3195807/94‑1 (MQ=60)
                                                                                  TAATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAG            <  SRR4302536.3213862/94‑1 (MQ=60)
                                                                                   AATGGCTCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGA           >  SRR4302536.4346978/1‑94 (MQ=60)
                                                                                   AATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGA           >  SRR4302536.1082188/1‑94 (MQ=60)
                                                                                   AATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGA           >  SRR4302536.1142768/1‑94 (MQ=60)
                                                                                   AATGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGA           >  SRR4302536.6229157/1‑94 (MQ=60)
                                                                                     TGGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGA         >  SRR4302536.5336155/1‑94 (MQ=60)
                                                                                      GGCCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAACCCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGAT        <  SRR4302536.3582125/94‑1 (MQ=60)
                                                                                        GCCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGA      <  SRR4302536.226523/94‑1 (MQ=60)
                                                                                         CCCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAC     <  SRR4302536.2961323/94‑1 (MQ=60)
                                                                                         CCCAC‑GCAACGGGAAGTCCCGCGTGCTCAGTAGGCCGGCGCTCTTGAAACCAAGAGGACGAAGATCCGCGAAGGTAAAGGAGAGGAGAGATGAC     <  SRR4302536.531669/94‑1 (MQ=42)
                                                                                          CCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACG    <  SRR4302536.6514895/94‑1 (MQ=60)
                                                                                          CCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACG    <  SRR4302536.3631410/94‑1 (MQ=60)
                                                                                          CCAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACG    <  SRR4302536.6260523/94‑1 (MQ=60)
                                                                                           CATCGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACG    <  SRR4302536.6514896/94‑1 (MQ=60)
                                                                                           CAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGC   >  SRR4302536.3452790/1‑94 (MQ=60)
                                                                                           CAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGC   >  SRR4302536.4520723/1‑94 (MQ=60)
                                                                                           CAC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGC   >  SRR4302536.1891062/1‑94 (MQ=60)
                                                                                            AC‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCC  <  SRR4302536.5766216/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGACGAAATATTGTTCAACTGCAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCAT‑GCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCC  >  NC_000913/2074152‑2074337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑