Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,074,403 T→C V25A (GTG→GCG)  yeeS → putative DNA repair protein, RADC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,4030TC98.0% 316.0 / ‑3.2 102V25A (GTG→GCG) yeeSputative DNA repair protein, RADC family
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (50/50);  total (52/50)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 108 total aligned reads displayed.

GTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTG  >  NC_000913/2074314‑2074491
                                                                                         |                                                                                        
gTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGc                                                                                       <  2:2345227/93‑1 (MQ=255)
  aaaGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCt                                                                                     <  2:4506073/93‑1 (MQ=255)
  aaaGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCt                                                                                     <  2:3592941/93‑1 (MQ=255)
   aaGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTAGACCGCCATCTTCATGAACCCGGCGCGGCCtt                                                                                    >  2:271737/1‑93 (MQ=255)
     gtagagtagaGATGACGCACGGCGAGCGCAGCCTCTTTCAACGGGCCCTGAAAACCCTGGCCCGCCATCTTCATGAGCCCGGCGCGGCCTTCa                                                                                  <  2:4173382/93‑1 (MQ=255)
     gTAGAGGAGAGATGACGCCCGACGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCa                                                                                  >  2:4659139/1‑93 (MQ=255)
      tAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAc                                                                                 >  2:1806273/1‑93 (MQ=255)
      tAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAc                                                                                 <  1:2621851/93‑1 (MQ=255)
       agagGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCAcc                                                                                <  2:1917177/93‑1 (MQ=255)
        gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAACACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                               >  2:839865/1‑93 (MQ=255)
        gaggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCt                                                                               >  2:3495929/1‑93 (MQ=255)
         aggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTc                                                                              >  1:3872565/1‑93 (MQ=255)
          ggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTcc                                                                             <  1:651216/93‑1 (MQ=255)
          ggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTcc                                                                             <  1:1757716/93‑1 (MQ=255)
          ggagAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTcc                                                                             >  2:3869440/1‑93 (MQ=255)
            agagaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAc                                                                           <  1:47085/93‑1 (MQ=255)
              agaTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCAccc                                                                         <  2:1643678/93‑1 (MQ=255)
                aTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCgg                                                                       >  1:404393/1‑92 (MQ=255)
                aTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGt                                                                       >  1:551088/1‑93 (MQ=255)
                aTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGt                                                                       <  1:3031299/93‑1 (MQ=255)
                     gCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTgcagc                                                                  >  1:3104316/1‑93 (MQ=255)
                     gCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagc                                                                  <  2:3260137/93‑1 (MQ=255)
                     gCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagc                                                                  >  2:1741259/1‑93 (MQ=255)
                      cccGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTgcagca                                                                 >  1:4676077/1‑93 (MQ=255)
                         ggCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACgg                                                              <  2:3024299/93‑1 (MQ=255)
                          gCGAGCGCAGCCTCATTCAACGGACCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACggg                                                             <  1:3437500/93‑1 (MQ=255)
                           cGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGa                                                            >  1:2456381/1‑93 (MQ=255)
                            gAGCGCAGCCTCATTCAACGGGCTCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGaa                                                           <  2:2580825/93‑1 (MQ=255)
                            gAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGaa                                                           <  1:2961267/93‑1 (MQ=255)
                             aGCGCAGCCTGATTCAACGGGCGCTGAAACCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCTCCTCCACCCGTGCAGCCCGGGAAt                                                          <  2:2860557/93‑1 (MQ=255)
                             aGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAt                                                          <  1:2345636/93‑1 (MQ=255)
                             aGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAt                                                          >  1:3976064/1‑93 (MQ=255)
                                gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                       >  1:1094767/1‑93 (MQ=255)
                                gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                       >  1:3519572/1‑93 (MQ=255)
                                gcAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                       <  1:2617622/93‑1 (MQ=255)
                                ccAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                                                       <  2:1695549/92‑1 (MQ=255)
                                 cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                      <  2:770808/93‑1 (MQ=255)
                                 cAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCt                                                      <  2:182715/93‑1 (MQ=255)
                                   gCCTCATTCAACGGGCCCTGAAACCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                    <  1:2495662/93‑1 (MQ=255)
                                   gCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGa                                                    >  1:2768458/1‑93 (MQ=255)
                                    ccTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAt                                                   <  1:1059648/93‑1 (MQ=255)
                                      tCATTCAACGGGCCCTGAAATCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                 <  2:677251/93‑1 (MQ=255)
                                      tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                 >  1:1467622/1‑93 (MQ=255)
                                      tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                 >  2:2560105/1‑93 (MQ=255)
                                      tCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                 >  1:928251/1‑93 (MQ=255)
                                      ccATCCAACGGGCCCTGAAAGCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                                                 <  2:1096175/92‑1 (MQ=255)
                                       cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                >  2:363273/1‑93 (MQ=255)
                                       cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                >  2:716447/1‑93 (MQ=255)
                                       cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                >  1:3764199/1‑93 (MQ=255)
                                       cATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                                                >  2:1430546/1‑93 (MQ=255)
                                         ttCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGa                                              >  2:3247554/1‑93 (MQ=255)
                                         ttCAACGGGCCCTGAAAACCCTGGAACGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCGCCCGTGCAGCACGGGAATGGCTGATTCTGa                                              <  1:3784662/93‑1 (MQ=255)
                                          tCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGaa                                             <  2:4449554/93‑1 (MQ=255)
                                             aCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGATGATTCTGAACAt                                          >  1:1766977/1‑93 (MQ=255)
                                              cGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATg                                         <  2:3571766/93‑1 (MQ=255)
                                              cGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATg                                         <  1:4222567/93‑1 (MQ=255)
                                              cGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATg                                         <  2:2610060/93‑1 (MQ=255)
                                              cGGGCCCTGAAAACCCAGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATg                                         >  2:1024146/1‑93 (MQ=255)
                                                ggCCCTGAAACCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                       <  2:4352816/93‑1 (MQ=255)
                                                ggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                       <  1:618790/93‑1 (MQ=255)
                                                ggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                       <  1:1437294/93‑1 (MQ=255)
                                                ggCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGc                                       >  2:2878832/1‑93 (MQ=255)
                                                 gCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCg                                      >  1:1554722/1‑93 (MQ=255)
                                                  cccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgg                                     <  2:1503094/93‑1 (MQ=255)
                                                   ccTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCggg                                    >  1:3288032/1‑93 (MQ=255)
                                                    cTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgggg                                   >  1:79790/1‑92 (MQ=255)
                                                    cTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGa                                   >  1:4711865/1‑93 (MQ=255)
                                                     tGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAc                                  <  1:4075441/93‑1 (MQ=255)
                                                      gAAAACCCTGGACCGCCAACTTCATGAGCCCGGCGCGGCATTCACCTCCCCCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACt                                 <  1:2438949/93‑1 (MQ=255)
                                                       aaaaCCCTGGACCGCCATCTTCATGATCCCGGCGCGGCCTTCACCTCCCCCCGTGCAGTACGAGAATGGCTGATTCTGAACATGGCGGGACTg                                <  2:3066328/93‑1 (MQ=255)
                                                        aaaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTgg                               <  1:447816/93‑1 (MQ=255)
                                                         aaCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGa                              >  1:1961580/1‑93 (MQ=255)
                                                           ccccGGCCCGTCATCTTCAGGAACCCGGCGCGGCCTTCCCCTCCACCCGTGCAGCACGGGCATGGCTGATTCTGAACATGGCGGGACTGGAGc                            <  1:3613008/93‑1 (MQ=255)
                                                             cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGGCCGGAGCgg                          >  1:3644816/1‑92 (MQ=255)
                                                             cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCgg                          >  1:3710328/1‑92 (MQ=255)
                                                             cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGt                          <  1:3864806/93‑1 (MQ=255)
                                                             cTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGt                          <  1:3355348/93‑1 (MQ=255)
                                                                   cGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaagaa                    <  2:2255593/93‑1 (MQ=255)
                                                                   cGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaagaa                    <  1:4008247/93‑1 (MQ=255)
                                                                   cGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTgaagaa                    >  2:258792/1‑93 (MQ=255)
                                                                    gCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAt                   >  1:611033/1‑93 (MQ=255)
                                                                    gCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAt                   >  2:3583869/1‑93 (MQ=255)
                                                                     ccATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAtt                  >  2:3916049/1‑93 (MQ=255)
                                                                      cATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTc                 >  1:3636045/1‑93 (MQ=255)
                                                                       aTCTTCATGAACCCGGCGCGGCCTTCACCTCCAGCCGTGCAGCACGGGAATGGCTGATTCTGAATATGGCTGGTCTGGAGCGGGAAGAATAcc                >  2:641110/1‑93 (MQ=255)
                                                                        tCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCg               <  2:3634141/93‑1 (MQ=255)
                                                                          ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg             <  1:2109200/93‑1 (MQ=255)
                                                                          ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg             <  2:3555448/93‑1 (MQ=255)
                                                                          ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg             >  2:3092593/1‑93 (MQ=255)
                                                                          ttCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggg             >  2:1817512/1‑93 (MQ=255)
                                                                           tCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGt            <  1:4442576/93‑1 (MQ=255)
                                                                            cATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCggggg           >  2:495027/1‑91 (MQ=255)
                                                                            cATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCCTGAAAAATTCCGGGTg           >  2:1321803/1‑93 (MQ=255)
                                                                             aTGATCCCGCCGCGGCTTTCACCTCAACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGc          <  2:637/93‑1 (MQ=37)
                                                                             aTGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGc          >  2:4410862/1‑93 (MQ=255)
                                                                              tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGCCTGGAGCGTGAAGAATTCCGGGTGCt         >  1:1657036/1‑93 (MQ=255)
                                                                              tGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCt         >  1:2736260/1‑93 (MQ=255)
                                                                                aaCCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGt       <  1:2774343/93‑1 (MQ=255)
                                                                                   ccGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATc    <  2:414755/93‑1 (MQ=255)
                                                                                     ggCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTg  <  1:591730/93‑1 (MQ=255)
                                                                                         |                                                                                        
GTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTG  >  NC_000913/2074314‑2074491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACA  >  NC_000913/2074310‑2074495
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GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGC                                                                                              >  SRR4302544.804685/1‑94 (MQ=60)
          TAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACC                                                                                    <  SRR4302544.2646996/94‑1 (MQ=60)
            GAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTC                                                                                  >  SRR4302544.3911024/1‑94 (MQ=60)
              GGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCA                                                                                <  SRR4302544.657663/94‑1 (MQ=60)
              GGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCA                                                                                <  SRR4302544.1774918/94‑1 (MQ=60)
                AGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACC                                                                              <  SRR4302544.47554/94‑1 (MQ=60)
                   GATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGT                                                                           >  SRR4302544.556484/1‑94 (MQ=60)
                   GATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGG                                                                           >  SRR4302544.408496/1‑94 (MQ=60)
                    ATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTG                                                                          <  SRR4302544.3060342/94‑1 (MQ=60)
                    ATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTC                                                                          >  SRR4302544.867640/1‑94 (MQ=60)
                        CGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGC                                                                      >  SRR4302544.3133863/1‑94 (MQ=60)
                         GCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCA                                                                     >  SRR4302544.4722292/1‑94 (MQ=60)
                              GCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGA                                                                >  SRR4302544.2479814/1‑94 (MQ=60)
                              GCGAGCGCAGCCTCATTCAACGGACCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGA                                                                <  SRR4302544.3470368/94‑1 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                              >  SRR4302544.4015629/1‑94 (MQ=60)
                                GAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAAT                                                              <  SRR4302544.2989692/94‑1 (MQ=60)
                                 AGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATG                                                             <  SRR4302544.2367859/94‑1 (MQ=60)
                                   CGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGC                                                           >  SRR4302544.3553183/1‑94 (MQ=60)
                                   CGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGC                                                           >  SRR4302544.1105661/1‑94 (MQ=60)
                                    GCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCT                                                          <  SRR4302544.2642745/94‑1 (MQ=60)
                                      AGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGA                                                        >  SRR4302544.2795092/1‑94 (MQ=60)
                                       GCCTCATTCAACGGGCCCTGAAACCCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGAT                                                       <  SRR4302544.2519573/94‑1 (MQ=60)
                                        CCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATT                                                      <  SRR4302544.1070172/94‑1 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                     >  SRR4302544.937395/1‑94 (MQ=60)
                                         CTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTC                                                     >  SRR4302544.1482171/1‑94 (MQ=60)
                                          TCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCT                                                    >  SRR4302544.3801545/1‑94 (MQ=60)
                                             TTCAACGGGCCCTGAAAACCCTGGAACGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCGCCCGTGCAGCACGGGAATGGCTGATTCTGAA                                                 <  SRR4302544.3822135/94‑1 (MQ=60)
                                                AACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGATGATTCTGAACAT                                              >  SRR4302544.1784272/1‑94 (MQ=60)
                                                  CGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGG                                            <  SRR4302544.4265423/94‑1 (MQ=60)
                                                    GGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCG                                          >  SRR4302544.1570190/1‑94 (MQ=60)
                                                    GGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCG                                          <  SRR4302544.624864/94‑1 (MQ=60)
                                                    GGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCG                                          <  SRR4302544.1451603/94‑1 (MQ=60)
                                                     GCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCACGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGG                                         <  SRR4302544.423628/94‑1 (MQ=60)
                                                      CCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGG                                        >  SRR4302544.3319360/1‑94 (MQ=60)
                                                       CCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGG                                       >  SRR4302544.80562/1‑94 (MQ=60)
                                                       CCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGA                                       >  SRR4302544.4758411/1‑94 (MQ=60)
                                                        CTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGAC                                      >  SRR4302544.2501646/1‑94 (MQ=60)
                                                         TGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACT                                     <  SRR4302544.4115995/94‑1 (MQ=60)
                                                          GAAAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTG                                    <  SRR4302544.3514873/94‑1 (MQ=60)
                                                          GAAAACCCTGGACCGCCAACTTCATGAGCCCGGCGCGGCATTCACCTCCCCCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTG                                    <  SRR4302544.2462198/94‑1 (MQ=60)
                                                            AAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGA                                  >  SRR4302544.1980619/1‑94 (MQ=60)
                                                            AAACCCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGA                                  <  SRR4302544.452272/94‑1 (MQ=60)
                                                               CCCCGGCCCGTCATCTTCAGGAACCCGGCGCGGCCTTCCCCTCCACCCGTGCAGCACGGGCATGGCTGATTCTGAACATGGCGGGACTGGAGCG                               <  SRR4302544.3648444/94‑1 (MQ=60)
                                                                CCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGgccggagcgg                              >  SRR4302544.3681182/1‑84 (MQ=60)
                                                                CCTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGG                              >  SRR4302544.3747236/1‑94 (MQ=60)
                                                                 CTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTG                             <  SRR4302544.3387366/94‑1 (MQ=60)
                                                                 CTGGACCGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTG                             <  SRR4302544.3903179/94‑1 (MQ=60)
                                                                       CGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAT                       <  SRR4302544.4048126/94‑1 (MQ=60)
                                                                       CGCCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAAT                       >  SRR4302544.617032/1‑94 (MQ=60)
                                                                         CCATCTTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTC                     >  SRR4302544.3672312/1‑94 (MQ=60)
                                                                              TTCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGT                <  SRR4302544.2129488/94‑1 (MQ=60)
                                                                               TCATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTG               <  SRR4302544.4487360/94‑1 (MQ=60)
                                                                                 ATGAAGCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGGCTGGGGCGTGAAGAATTCCGGGggcg             >  SRR4302544.3560884/1‑90 (MQ=60)
                                                                                 ATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGCCTGGAGCGTGAAGAATTCCGGGTGCT             >  SRR4302544.1673366/1‑94 (MQ=60)
                                                                                 ATGAACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCT             >  SRR4302544.2762636/1‑94 (MQ=60)
                                                                                    AACCCGGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTA          <  SRR4302544.2801026/94‑1 (MQ=60)
                                                                                         GGCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGA     <  SRR4302544.597540/94‑1 (MQ=60)
                                                                                          GCGCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAA    >  SRR4302544.2530704/1‑94 (MQ=60)
                                                                                            GCGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACA  <  SRR4302544.182523/94‑1 (MQ=60)
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GAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACA  >  NC_000913/2074310‑2074495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑