Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,092,192 A→G T235A (ACC→GCC)  b2022 → imidazoleglycerolphosphate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,092,1920AG100.0% 852.0 / NA 260T235A (ACC→GCC) b2022imidazoleglycerolphosphate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (129/131);  total (129/131)

Only 100 of 268 total aligned reads displayed.

TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092103‑2092281
                                                                                         |                                                                                         
tGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCg                                                                                        >  2:5018203/1‑93 (MQ=255)
 ggATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGCTGCCgg                                                                                       >  1:563494/1‑93 (MQ=255)
 ggATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCgg                                                                                       >  2:6249776/1‑93 (MQ=255)
   aTCAGATCGCTGCCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     <  2:816727/93‑1 (MQ=255)
   aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     <  1:1635635/93‑1 (MQ=255)
   aTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGcc                                                                                     >  1:5844537/1‑93 (MQ=255)
    tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCt                                                                                    >  2:482177/1‑93 (MQ=255)
    tCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCt                                                                                    <  2:1273414/93‑1 (MQ=255)
     cAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTg                                                                                   >  2:6234796/1‑93 (MQ=255)
      aGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTgg                                                                                  <  1:1845834/93‑1 (MQ=255)
      aGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTgg                                                                                  >  1:2195380/1‑93 (MQ=255)
      aGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTgg                                                                                  >  1:3653489/1‑93 (MQ=255)
       gATCGCTACCCACGGCGGGTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgc                                                                                 >  1:3556307/1‑93 (MQ=255)
         tCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTggggt                                                                               >  1:4150755/1‑90 (MQ=255)
         tCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGgggc                                                                               >  2:4741352/1‑93 (MQ=255)
          cGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCt                                                                              >  2:3079291/1‑93 (MQ=255)
          cGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCt                                                                              <  2:1890299/93‑1 (MQ=255)
           gCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTg                                                                             >  1:487675/1‑93 (MQ=255)
            cTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTgg                                                                            <  1:477823/93‑1 (MQ=255)
            cTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTgg                                                                            <  2:3937438/93‑1 (MQ=255)
             tACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTggg                                                                           >  2:2794129/1‑93 (MQ=255)
             tACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTggg                                                                           >  2:1433061/1‑93 (MQ=255)
                ccACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGa                                                                        <  1:3266725/93‑1 (MQ=255)
                 cACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGaa                                                                       <  2:4943295/93‑1 (MQ=255)
                 cACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGaa                                                                       >  2:5285239/1‑93 (MQ=255)
                  aCGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAg                                                                      >  1:6110195/1‑93 (MQ=255)
                  aCGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAg                                                                      >  2:2285129/1‑93 (MQ=255)
                   cggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgg                                                                     >  1:2995753/1‑92 (MQ=255)
                   cggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgc                                                                     <  2:3155262/93‑1 (MQ=255)
                   cggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgc                                                                     <  1:6588830/93‑1 (MQ=255)
                    ggcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTCGGCGAAgcg                                                                    >  1:4376408/1‑93 (MQ=255)
                     gcggTTTCCGCATGGAAATCAACGTCAAAGGCGCACTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgcgc                                                                   <  2:5821684/93‑1 (MQ=255)
                     gcggTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAgcgc                                                                   <  1:6421414/93‑1 (MQ=255)
                        gTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaa                                                                >  1:5531623/1‑93 (MQ=255)
                         tttCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaa                                                               <  2:5991621/93‑1 (MQ=255)
                           tCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaaaa                                                             <  2:576536/93‑1 (MQ=255)
                           tCCGCATGGAAATCAACGTCAAAGCCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTaaaaa                                                             <  1:830735/93‑1 (MQ=255)
                             cGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATc                                                           >  1:1018820/1‑93 (MQ=255)
                              gCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCg                                                          <  2:5938195/93‑1 (MQ=255)
                              gCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCg                                                          >  1:1530880/1‑93 (MQ=255)
                              gCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGAGAAGCGCTAAAAATCg                                                          >  1:2811937/1‑93 (MQ=255)
                                aTGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGTGCTGGGCGAAGCGCTAAAAATAGcc                                                        >  1:249882/1‑93 (MQ=255)
                                aTGGAAATCAACGTCAAAGGCGACCTCGATATCTTCGATCACCACACCGTCGAAGATGACGGCCTCGCGCTGGGCGAAGCGCTAAAAATCGcc                                                        >  2:2116397/1‑93 (MQ=255)
                                  ggAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCt                                                      <  1:1389222/93‑1 (MQ=255)
                                  ggAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCt                                                      <  2:1989166/93‑1 (MQ=255)
                                  ggAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATCGCCCt                                                      >  2:3389401/1‑93 (MQ=255)
                                    aaaTCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCg                                                    <  1:580313/93‑1 (MQ=255)
                                       tCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGgag                                                 <  2:2251118/93‑1 (MQ=255)
                                        cAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGgagc                                                >  2:1633702/1‑92 (MQ=255)
                                        cAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGgaga                                                >  2:4346731/1‑93 (MQ=255)
                                          aCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACa                                              >  2:666589/1‑93 (MQ=255)
                                          aCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACa                                              >  2:6721664/1‑93 (MQ=255)
                                          aCGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACa                                              >  2:848657/1‑93 (MQ=255)
                                           cGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaa                                             <  2:4125799/93‑1 (MQ=255)
                                           cGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACaa                                             <  1:2692647/93‑1 (MQ=255)
                                           cGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTCGGCGAAGCGCTAAAAATCGCCCTCGGAGACaa                                             >  2:3518600/1‑93 (MQ=255)
                                             tCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAc                                           >  2:2441380/1‑93 (MQ=255)
                                               aaaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgc                                         <  2:1604988/93‑1 (MQ=255)
                                               aaaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgc                                         >  2:5255583/1‑93 (MQ=255)
                                                aaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgcg                                        >  2:643606/1‑93 (MQ=255)
                                                aaGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAAcgcg                                        >  1:6629491/1‑93 (MQ=255)
                                                  ggCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGt                                      >  1:3849247/1‑93 (MQ=255)
                                                     gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   <  2:4264700/93‑1 (MQ=255)
                                                     gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   <  1:738497/93‑1 (MQ=255)
                                                     gACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAtt                                   <  2:5120204/93‑1 (MQ=255)
                                                      gccTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  <  1:5331592/92‑1 (MQ=255)
                                                      aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  <  2:2373418/93‑1 (MQ=255)
                                                      aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  >  1:243776/1‑93 (MQ=255)
                                                      aCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTAttt                                  >  1:235863/1‑93 (MQ=255)
                                                       ccTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTg                                 <  2:5527942/93‑1 (MQ=255)
                                                       ccTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTg                                 <  1:4712264/93‑1 (MQ=255)
                                                        ctctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGc                                <  2:3765977/93‑1 (MQ=255)
                                                        ctctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGc                                <  2:469023/93‑1 (MQ=255)
                                                         tctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGcc                               >  1:3197976/1‑93 (MQ=255)
                                                         tctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGcc                               <  1:1054178/93‑1 (MQ=255)
                                                         tctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGcc                               >  2:2920377/1‑93 (MQ=255)
                                                          ctATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCg                              <  2:2645930/93‑1 (MQ=255)
                                                             tatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCtt                           >  2:4486707/1‑93 (MQ=255)
                                                             tatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCtt                           >  2:3609520/1‑93 (MQ=255)
                                                             tatCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAAAAACGCGGTATTTGCCGCtt                           >  1:886073/1‑93 (MQ=255)
                                                              atCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCttt                          <  1:4542186/93‑1 (MQ=255)
                                                               tCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTg                         >  2:5641148/1‑93 (MQ=255)
                                                               tCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTg                         <  1:1803496/93‑1 (MQ=255)
                                                                 gacgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGt                       >  1:4426085/1‑93 (MQ=255)
                                                                 gacgaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGt                       <  2:6352907/93‑1 (MQ=255)
                                                                    gaTCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGtttt                    >  2:3956914/1‑93 (MQ=255)
                                                                     aTCACCACCCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtg                   >  1:6565345/1‑93 (MQ=255)
                                                                      tCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTtgt                  <  2:6377556/93‑1 (MQ=255)
                                                                        accacACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGc                <  2:3288120/93‑1 (MQ=255)
                                                                          cacaCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTa              >  1:3038327/1‑93 (MQ=255)
                                                                           acacCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGAATTTGCCGCTTTGGTTTTGTGCTAc             >  2:6526305/1‑93 (MQ=255)
                                                                             acCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCg           >  1:5051465/1‑93 (MQ=255)
                                                                             acCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCg           >  2:554872/1‑93 (MQ=255)
                                                                                gTCGAAGATGCCGGCCTGGCGCTGGTCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        <  1:5941303/93‑1 (MQ=255)
                                                                                gTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATg        <  2:4601045/93‑1 (MQ=255)
                                                                                     aGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGa   >  2:2969442/1‑93 (MQ=255)
                                                                                      gATGCCGGCCTGGCTCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTGGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:2076318/93‑1 (MQ=255)
                                                                                      gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  2:2584861/93‑1 (MQ=255)
                                                                                      gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  >  1:356178/1‑93 (MQ=255)
                                                                                      gATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGaa  <  1:4641/93‑1 (MQ=255)
                                                                                         |                                                                                         
TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA  >  NC_000913/2092103‑2092281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  >  NC_000913/2092099‑2092282
                                                                                             |                                                                                          
ATGCTGGTTCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                            >  SRR4302536.3333242/1‑94 (MQ=60)
ATGCTGGATCAGATCGCTGCCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                            <  SRR4302536.2559723/94‑1 (MQ=60)
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                            <  SRR4302536.5258340/94‑1 (MQ=60)
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATG                                                                                            <  SRR4302536.1753405/94‑1 (MQ=60)
  TCTGGATCAGATCGCTTCCCACGTCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCC                                                                                          <  SRR4302536.3568321/94‑1 (MQ=60)
    TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGCTGCCGG                                                                                        >  SRR4302536.577847/1‑94 (MQ=60)
    TGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGG                                                                                        >  SRR4302536.4344057/1‑94 (MQ=60)
      GATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCC                                                                                      >  SRR4302536.6018675/1‑94 (MQ=60)
       ATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCT                                                                                     <  SRR4302536.1683260/94‑1 (MQ=60)
        TCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATACCGACGATCACCACACCGTCGAAGATGCCGGCCTG                                                                                    <  SRR4302536.4877215/94‑1 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                   >  SRR4302536.3762711/1‑94 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                   >  SRR4302536.2261642/1‑94 (MQ=60)
         CAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGG                                                                                   >  SRR4302536.6917492/1‑94 (MQ=60)
          AGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGC                                                                                  <  SRR4302536.3215298/94‑1 (MQ=60)
          AGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGC                                                                                  <  SRR4302536.1901442/94‑1 (MQ=60)
          AGATCGCTACCCACGGCGGGTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGC                                                                                  >  SRR4302536.3662647/1‑94 (MQ=60)
           GATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAG                                                                                 >  SRR4302536.3116304/1‑94 (MQ=60)
            ATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGggt                                                                                >  SRR4302536.4273946/1‑91 (MQ=60)
            ATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGC                                                                                >  SRR4302536.33437/1‑94 (MQ=60)
             TCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCT                                                                               <  SRR4302536.161752/94‑1 (MQ=60)
             TCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCT                                                                               <  SRR4302536.4851253/94‑1 (MQ=60)
              CGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTG                                                                              >  SRR4302536.500324/1‑94 (MQ=60)
               GCTACCCACGGCGGTTTCCGCGTGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGG                                                                             <  SRR4302536.6134554/94‑1 (MQ=60)
               GCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGG                                                                             >  SRR4302536.1877868/1‑94 (MQ=60)
               GCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCCGGCGCTGG                                                                             <  SRR4302536.6803144/94‑1 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                            >  SRR4302536.4898144/1‑94 (MQ=60)
                CTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGG                                                                            <  SRR4302536.490266/94‑1 (MQ=60)
                 TCCCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGC                                                                           <  SRR4302536.587574/94‑1 (MQ=60)
                    CCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAA                                                                        <  SRR4302536.3362620/94‑1 (MQ=60)
                     CACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAG                                                                       >  SRR4302536.6292342/1‑94 (MQ=60)
                      ACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGG                                                                      >  SRR4302536.3083858/1‑94 (MQ=60)
                      ACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGC                                                                      <  SRR4302536.4757628/94‑1 (MQ=60)
                       CGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCG                                                                     <  SRR4302536.6784836/94‑1 (MQ=60)
                       CGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTCGGCGAAGCG                                                                     >  SRR4302536.4507093/1‑94 (MQ=60)
                         GCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCT                                                                   <  SRR4302536.6613332/94‑1 (MQ=60)
                           GGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGGGAAGAGCTAA                                                                 >  SRR4302536.4822150/1‑94 (MQ=60)
                           GGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAA                                                                 >  SRR4302536.5696526/1‑94 (MQ=60)
                           CGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAA                                                                 <  SRR4302536.881490/94‑1 (MQ=60)
                               TCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACCCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAAT                                                             <  SRR4302536.3307638/94‑1 (MQ=60)
                               TCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAAT                                                             <  SRR4302536.2518397/94‑1 (MQ=60)
                               TCCGCATGGAAATCAACGTCAAAGCCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAAT                                                             <  SRR4302536.855187/94‑1 (MQ=60)
                                CCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATC                                                            >  SRR4302536.1048963/1‑94 (MQ=60)
                                CCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGAGCTGGGCGAAGCGCTAAAAATC                                                            >  SRR4302536.3781635/1‑94 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCG                                                           >  SRR4302536.1575926/1‑94 (MQ=60)
                                 CGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGAGAAGCGCTAAAAATCG                                                           >  SRR4302536.2895277/1‑94 (MQ=60)
                                   CATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGTGCTGGGCGAAGCGCTAAAAATAGCC                                                         >  SRR4302536.256555/1‑94 (MQ=60)
                                   CATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCC                                                         >  SRR4302536.1706240/1‑94 (MQ=60)
                                   CATGGAAATAAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCC                                                         >  SRR4302536.6762239/1‑94 (MQ=60)
                                    ATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCC                                                        >  SRR4302536.1508960/1‑94 (MQ=60)
                                    ATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCC                                                        >  SRR4302536.5289746/1‑94 (MQ=60)
                                     TGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCT                                                       >  SRR4302536.3098206/1‑94 (MQ=60)
                                     GGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCT                                                       <  SRR4302536.3708431/94‑1 (MQ=60)
                                      GGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTC                                                      <  SRR4302536.504045/94‑1 (MQ=60)
                                      GGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTC                                                      <  SRR4302536.4950716/94‑1 (MQ=60)
                                      GGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTC                                                      <  SRR4302536.1430592/94‑1 (MQ=60)
                                      GGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGAAGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTC                                                      <  SRR4302536.2513325/94‑1 (MQ=60)
                                       GAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCG                                                     <  SRR4302536.1341700/94‑1 (MQ=60)
                                       GAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCG                                                     <  SRR4302536.2755265/94‑1 (MQ=60)
                                        AAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGG                                                    <  SRR4302536.595042/94‑1 (MQ=60)
                                         AATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGGGCTGGGCGAAGCGCTAAAAATCGCCCTCGGA                                                   >  SRR4302536.6508960/1‑94 (MQ=60)
                                         AATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGA                                                   <  SRR4302536.547099/94‑1 (MQ=60)
                                           TCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGA                                                 >  SRR4302536.6022421/1‑94 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGACGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                >  SRR4302536.6778458/1‑94 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                <  SRR4302536.4628776/94‑1 (MQ=60)
                                            CAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAC                                                >  SRR4302536.3014509/1‑94 (MQ=60)
                                               CGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAA                                             <  SRR4302536.2773036/94‑1 (MQ=60)
                                                 GCAAAGGCGACCTCTATATCGACGATCCCCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACG                                           <  SRR4302536.2203693/94‑1 (MQ=60)
                                                   AAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCG                                         >  SRR4302536.6826681/1‑94 (MQ=60)
                                                   AAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCG                                         <  SRR4302536.6237500/94‑1 (MQ=60)
                                                     AGGCGACCTCTATATCGACGATCACCACACCTTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGT                                       <  SRR4302536.5702112/94‑1 (MQ=60)
                                                     AGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGT                                       >  SRR4302536.3963841/1‑94 (MQ=60)
                                                      GGCGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTA                                      <  SRR4302536.2487243/94‑1 (MQ=60)
                                                        CGACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATT                                    >  SRR4302536.802341/1‑94 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                   <  SRR4302536.760143/94‑1 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                   >  SRR4302536.242131/1‑94 (MQ=60)
                                                         GACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTT                                   >  SRR4302536.250277/1‑94 (MQ=60)
                                                          GCCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTG                                  <  SRR4302536.5490884/94‑1 (MQ=60)
                                                          ACCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTG                                  >  SRR4302536.1711224/1‑94 (MQ=60)
                                                           CCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGC                                 <  SRR4302536.2271072/94‑1 (MQ=60)
                                                           CCTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGC                                 <  SRR4302536.4854003/94‑1 (MQ=60)
                                                            CTCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCC                                >  SRR4302536.3291726/1‑94 (MQ=60)
                                                             TCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCG                               <  SRR4302536.1085680/94‑1 (MQ=60)
                                                             TCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCG                               <  SRR4302536.2884363/94‑1 (MQ=60)
                                                             TCTATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCG                               <  SRR4302536.6040480/94‑1 (MQ=60)
                                                               TATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATAGCCCTCGGAGACAAACGCGGTATTTGCCGCT                             >  SRR4302536.5428053/1‑94 (MQ=60)
                                                                ATATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGAAAAACGCGGTATTTGCCGCTT                            >  SRR4302536.912146/1‑94 (MQ=60)
                                                                 TATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTT                           >  SRR4302536.2394272/1‑94 (MQ=60)
                                                                  ATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTG                          <  SRR4302536.4678379/94‑1 (MQ=60)
                                                                  ATCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTG                          >  SRR4302536.6017753/1‑94 (MQ=60)
                                                                   TCGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGG                         <  SRR4302536.1857936/94‑1 (MQ=60)
                                                                    CGACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGT                        >  SRR4302536.4558692/1‑94 (MQ=60)
                                                                     GACGATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTT                       <  SRR4302536.5069894/94‑1 (MQ=60)
                                                                        GATCACCACCCCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTG                    >  SRR4302536.6760762/1‑94 (MQ=60)
                                                                        GATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTG                    >  SRR4302536.3822250/1‑94 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                   <  SRR4302536.1898735/94‑1 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                   <  SRR4302536.6859280/94‑1 (MQ=60)
                                                                         ATCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGT                   >  SRR4302536.3663021/1‑94 (MQ=60)
                                                                          TCACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTG                  <  SRR4302536.5781832/94‑1 (MQ=60)
                                                                           CACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGG                 >  SRR4302536.406116/1‑94 (MQ=60)
                                                                           CACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGC                 >  SRR4302536.360189/1‑94 (MQ=60)
                                                                           CACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGC                 >  SRR4302536.2974379/1‑94 (MQ=60)
                                                                            ACCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCT                <  SRR4302536.6672208/94‑1 (MQ=60)
                                                                             CCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTA               >  SRR4302536.3127664/1‑94 (MQ=60)
                                                                             CCACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTA               <  SRR4302536.4543080/94‑1 (MQ=60)
                                                                              CACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAC              >  SRR4302536.1454272/1‑94 (MQ=60)
                                                                              CACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTAC              >  SRR4302536.4624865/1‑94 (MQ=60)
                                                                               ACACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACC             >  SRR4302536.1059936/1‑94 (MQ=60)
                                                                                CACCGTCGAAGATGCCGGCGTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCG            <  SRR4302536.4361120/94‑1 (MQ=60)
                                                                                CACCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCG            >  SRR4302536.5202821/1‑94 (MQ=60)
                                                                                CACCGACGAAGATGCCGTCCGGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGTAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTTCTACCG            <  SRR4302536.6880139/94‑1 (MQ=60)
                                                                                 ACCGTCGAAGATGCCGGCCTCGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGA           >  SRR4302536.6716973/1‑94 (MQ=60)
                                                                                  CCGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGAT          <  SRR4302536.2766089/94‑1 (MQ=60)
                                                                                   CGTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAATCGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATG         <  SRR4302536.4532263/94‑1 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGTCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG        <  SRR4302536.6118311/94‑1 (MQ=60)
                                                                                    GTCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGG        <  SRR4302536.1421703/94‑1 (MQ=60)
                                                                                     TCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGA       >  SRR4302536.3407769/1‑94 (MQ=60)
                                                                                     TCGAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGA       >  SRR4302536.2112762/1‑94 (MQ=60)
                                                                                       GAAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACG     >  SRR4302536.2186228/1‑94 (MQ=60)
                                                                                        AAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGA    >  SRR4302536.786965/1‑94 (MQ=60)
                                                                                        AAGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGA    >  SRR4302536.374920/1‑94 (MQ=60)
                                                                                         AGATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAA   >  SRR4302536.365612/1‑94 (MQ=60)
                                                                                          GATGCCGGCCTGGCTCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTGGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  <  SRR4302536.2138852/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  <  SRR4302536.5903524/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  <  SRR4302536.4754/94‑1 (MQ=60)
                                                                                          GATGCCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  >  SRR4302536.6182464/1‑94 (MQ=60)
                                                                                             |                                                                                          
ATGCTGGATCAGATCGCTACCCACGGCGGTTTCCGCATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGCGAAGCGCTAAAAATCGCCCTCGGAGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAAT  >  NC_000913/2092099‑2092282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑