Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,539	T→C	V225A (GTT→GCT)	hisF →	imidazole glycerol phosphate synthase holoenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,539	0	T	C	100.0%	819.5 / NA	251	V225A (GTT→GCT)	hisF	imidazole glycerol phosphate synthase holoenzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/150); total (101/150)

Only 100 of 264 total aligned reads displayed.

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

aaGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTg                                                                                         >  1:2842159/1‑93 (MQ=255)

aaGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTg                                                                                         <  2:5807986/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  2:3346127/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:3736585/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:602753/93‑1 (MQ=255)

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        >  1:6164292/1‑93 (MQ=255)

  gTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAc                                                                                       <  1:5563304/93‑1 (MQ=255)

   tGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACg                                                                                      >  2:1802617/1‑93 (MQ=255)

   tGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACg                                                                                      >  2:1018846/1‑93 (MQ=255)

     cGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgc                                                                                    >  1:3069271/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  1:454825/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  2:612052/1‑93 (MQ=255)

       tGATGTTTGCCGCGTCCCTCTGATTGCCTTCTGTGGCGCGGGCTCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCTACGCTGACGgcgc                                                                                  <  1:6596564/93‑1 (MQ=255)

        gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTGGAAGCCTTCCGCGATGCCGACGCTGCCGgggcg                                                                                 >  2:5588706/1‑93 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcgc                                                                                <  1:5042406/93‑1 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcgc                                                                                >  1:6342553/1‑93 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgccctc                                                                                >  1:1269665/1‑89 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:3468600/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:2875810/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:4565826/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:508966/1‑93 (MQ=255)

             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            <  2:4931521/93‑1 (MQ=255)

              tGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCa                                                                           <  2:3611996/93‑1 (MQ=255)

               gCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAg                                                                          >  2:270640/1‑93 (MQ=255)

                ccACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGc                                                                         >  1:2926772/1‑93 (MQ=255)

                 cACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCCAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCt                                                                        >  1:6516890/1‑93 (MQ=255)

                  ccGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCtt                                                                       <  2:273294/92‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:1596990/93‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:4506513/93‑1 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTCCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  2:2508161/93‑1 (MQ=255)

                    gTCCCGCGGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTcc                                                                     <  1:2705531/93‑1 (MQ=255)

                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  2:2135683/1‑93 (MQ=255)

                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCgca                                                                  >  2:5832599/1‑91 (MQ=255)

                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  >  1:1646873/1‑93 (MQ=255)

                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:5106198/1‑93 (MQ=255)

                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:3857198/1‑93 (MQ=255)

                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAtt                                                                <  2:3933455/93‑1 (MQ=255)

                           tGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTcc                                                              <  1:2024944/93‑1 (MQ=255)

                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             >  1:3485510/1‑93 (MQ=255)

                             aTTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCcac                                                            <  2:6654918/93‑1 (MQ=255)

                               tGCCTCCGGTGGCGCGGGCACCATGGAACACTGCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaa                                                          <  1:690559/93‑1 (MQ=255)

                                gCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaa                                                         <  1:2661997/93‑1 (MQ=255)

                                    ccGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAcaaacaaa                                                     <  2:4996469/93‑1 (MQ=255)

                                     cGGTGGCGCGGGTACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:4501490/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  2:4656811/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    >  1:550192/1‑93 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:5305629/93‑1 (MQ=255)

                                      ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   >  2:2970828/1‑93 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  1:4212920/93‑1 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  2:810685/93‑1 (MQ=255)

                                              gggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAatat                                           <  1:1914909/93‑1 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTTCGTATTCCACAAACAAATAATCAATAtt                                          >  1:4694705/1‑93 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  1:6422313/93‑1 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  1:5242395/93‑1 (MQ=255)

                                                 cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTgg                                        <  1:3835375/93‑1 (MQ=255)

                                                 cACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCAACAAACAAATAATCAATATTgg                                        <  2:5667602/93‑1 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       >  1:1529151/1‑93 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       <  2:4291011/93‑1 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       >  1:464901/1‑93 (MQ=255)

                                                     aTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGaa                                    <  1:2189304/93‑1 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  2:1497935/93‑1 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   >  1:3381561/1‑93 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  2:2206633/93‑1 (MQ=255)

                                                       ggAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                  <  1:257893/93‑1 (MQ=255)

                                                       ggAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAtt                                  >  2:4380442/1‑93 (MQ=255)

                                                        gAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTa                                 <  2:5503568/93‑1 (MQ=255)

                                                          acacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaa                               <  2:6520006/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  1:4877062/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  2:2097363/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  2:226598/93‑1 (MQ=255)

                                                           cacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaaa                              <  1:2869796/93‑1 (MQ=255)

                                                            ccTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  1:4846346/92‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  2:1646873/93‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:814914/93‑1 (MQ=255)

                                                                ccTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTa                         <  2:6058672/93‑1 (MQ=255)

                                                                  tCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                       <  1:5204838/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  2:4015046/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  1:2361544/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      >  2:3256749/1‑93 (MQ=255)

                                                                    gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     <  2:899048/93‑1 (MQ=255)

                                                                     aaGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTgg                    >  1:3965208/1‑93 (MQ=255)

                                                                      aGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGc                   >  2:4183446/1‑93 (MQ=255)

                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  2:1375516/1‑93 (MQ=255)

                                                                       gCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCa                  >  1:2442968/1‑93 (MQ=255)

                                                                        ccTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCaa                 >  1:740316/1‑93 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               <  1:135722/93‑1 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  2:1055923/1‑93 (MQ=255)

                                                                            ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             >  2:5924305/1‑93 (MQ=255)

                                                                             cgcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAg            <  1:1006906/93‑1 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           >  1:4901713/1‑93 (MQ=255)

                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        <  2:820037/93‑1 (MQ=255)

                                                                                 aTGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCg        >  2:3083993/1‑93 (MQ=255)

                                                                                  tGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGt       <  1:1121348/93‑1 (MQ=255)

                                                                                  tGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGt       <  2:681931/93‑1 (MQ=255)

                                                                                   gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTg      >  2:3739736/1‑93 (MQ=255)

                                                                                   gCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGCTGAATTAAAAGCGTACCTGGCAACACAGCGCGTg      >  1:1855400/1‑93 (MQ=255)

                                                                                     cGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGga    <  2:6216455/93‑1 (MQ=255)

                                                                                      gACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   <  2:1220817/93‑1 (MQ=255)

                                                                                      gACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   >  2:2041579/1‑93 (MQ=255)

                                                                                      caCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgag   <  2:4881613/92‑1 (MQ=255)

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAG  >  NC_000913/2094446‑2094632

AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGC                                                                                               >  SRR4302536.6837166/1‑94 (MQ=60)

 AAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCT                                                                                              <  SRR4302536.1169431/94‑1 (MQ=60)

  AAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTG                                                                                             >  SRR4302536.2926246/1‑94 (MQ=60)

  AAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTG                                                                                             <  SRR4302536.4474199/94‑1 (MQ=60)

   AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGA                                                                                            <  SRR4302536.4424065/94‑1 (MQ=60)

   AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGA                                                                                            >  SRR4302536.5706744/1‑94 (MQ=60)

   AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGA                                                                                            >  SRR4302536.6347913/1‑94 (MQ=60)

    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                           <  SRR4302536.3277861/94‑1 (MQ=60)

    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                           <  SRR4302536.3848260/94‑1 (MQ=60)

    AGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                           <  SRR4302536.620437/94‑1 (MQ=60)

    AGGGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGAC                                                                                           <  SRR4302536.3603839/94‑1 (MQ=60)

     GTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACG                                                                                          >  SRR4302536.2689818/1‑94 (MQ=60)

     GTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACG                                                                                          >  SRR4302536.4725538/1‑94 (MQ=60)

     GTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACG                                                                                          <  SRR4302536.5728991/94‑1 (MQ=60)

       GCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGC                                                                                        >  SRR4302536.3159217/1‑94 (MQ=60)

        CGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCG                                                                                       >  SRR4302536.466750/1‑94 (MQ=60)

         GTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGC                                                                                      >  SRR4302536.2060317/1‑94 (MQ=60)

          TGATGTTTGCCGCGTCCCTCTGATTGCCTTCTGTGGCGCGGGCTCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCTACGCTGACGGCGCG                                                                                     <  SRR4302536.6792783/94‑1 (MQ=60)

           GAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCcctc                                                                                    >  SRR4302536.1307701/1‑90 (MQ=60)

           GAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGC                                                                                    >  SRR4302536.4245872/1‑94 (MQ=60)

           GAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGC                                                                                    >  SRR4302536.6531511/1‑94 (MQ=60)

            AAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCT                                                                                   >  SRR4302536.5339784/1‑94 (MQ=60)

            AAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCT                                                                                   <  SRR4302536.5193527/94‑1 (MQ=60)

             AGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTG                                                                                  >  SRR4302536.19952/1‑94 (MQ=60)

             AGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTG                                                                                  >  SRR4302536.3224360/1‑94 (MQ=60)

              GTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGG                                                                                 >  SRR4302536.586595/1‑94 (MQ=60)

               TTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGC                                                                                <  SRR4302536.6336228/94‑1 (MQ=60)

                TTGCCACGTCCCGCTGATTGCCTCCGGCGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCG                                                                               >  SRR4302536.4522339/1‑94 (MQ=60)

                  GCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGC                                                                             >  SRR4302536.3013144/1‑94 (MQ=60)

                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                            >  SRR4302536.6711058/1‑94 (MQ=60)

                   CCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCCAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCT                                                                            >  SRR4302536.6711057/1‑94 (MQ=60)

                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                           <  SRR4302536.865417/94‑1 (MQ=60)

                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                           >  SRR4302536.6538024/1‑94 (MQ=60)

                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                           >  SRR4302536.6824899/1‑94 (MQ=60)

                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                           <  SRR4302536.847841/94‑1 (MQ=60)

                    CACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTT                                                                           <  SRR4302536.1610272/94‑1 (MQ=60)

                     ACGCCCCGCTTATGGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTC                                                                          <  SRR4302536.1232824/94‑1 (MQ=60)

                      CGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCC                                                                         <  SRR4302536.2764570/94‑1 (MQ=60)

                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                        >  SRR4302536.1901049/1‑94 (MQ=60)

                       GTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                        >  SRR4302536.4775849/1‑94 (MQ=60)

                       GTCCCGCGGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCG                                                                        <  SRR4302536.2786258/94‑1 (MQ=60)

                         CCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTA                                                                      >  SRR4302536.1694760/1‑94 (MQ=60)

                         CCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTA                                                                      <  SRR4302536.4996191/94‑1 (MQ=60)

                          CCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAT                                                                     >  SRR4302536.3869622/1‑94 (MQ=60)

                          CCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAT                                                                     >  SRR4302536.285470/1‑94 (MQ=60)

                          CCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAT                                                                     <  SRR4302536.5404603/94‑1 (MQ=60)

                            GCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTC                                                                   >  SRR4302536.1346198/1‑94 (MQ=60)

                            GCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTC                                                                   <  SRR4302536.1948468/94‑1 (MQ=60)

                              TGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCA                                                                 <  SRR4302536.2085930/94‑1 (MQ=60)

                              TGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCA                                                                 >  SRR4302536.3589457/1‑94 (MQ=60)

                               GATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCAC                                                                <  SRR4302536.3994738/94‑1 (MQ=60)

                                  TGCCTCCGGTGGCGCGGGCACCATGGAACACTGCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAA                                                             <  SRR4302536.710757/94‑1 (MQ=60)

                                   GCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAAC                                                            <  SRR4302536.2741649/94‑1 (MQ=60)

                                    ccgcgGGTGGCGTGGGCACCATGGCACATTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACA                                                           <  SRR4302536.5894507/89‑1 (MQ=60)

                                    CCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACA                                                           <  SRR4302536.5225413/94‑1 (MQ=60)

                                     CTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAA                                                          <  SRR4302536.5377106/94‑1 (MQ=60)

                                     CTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAA                                                          <  SRR4302536.3156831/94‑1 (MQ=60)

                                      TCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAA                                                         >  SRR4302536.4970897/1‑94 (MQ=60)

                                       CCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAAT                                                        <  SRR4302536.772558/94‑1 (MQ=60)

                                       CCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAAT                                                        >  SRR4302536.564221/1‑94 (MQ=60)

                                        CGGTGGCGCGGGTACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                       <  SRR4302536.4636586/94‑1 (MQ=60)

                                        CGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                       <  SRR4302536.5051472/94‑1 (MQ=60)

                                        CGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                       <  SRR4302536.5463950/94‑1 (MQ=60)

                                        CGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATA                                                       <  SRR4302536.1643670/94‑1 (MQ=60)

                                         GGTGTCGCGAGCACCATGCAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAA                                                      <  SRR4302536.6684767/94‑1 (MQ=60)

                                           TGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATC                                                    <  SRR4302536.6904617/94‑1 (MQ=60)

                                             GCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAA                                                  <  SRR4302536.5790963/94‑1 (MQ=60)

                                              CGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAAT                                                 <  SRR4302536.5579753/94‑1 (MQ=60)

                                               GCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATA                                                <  SRR4302536.716051/94‑1 (MQ=60)

                                               CCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCTGTATTCCACAAACAAATAATCAATA                                                <  SRR4302536.6420300/94‑1 (MQ=60)

                                                CGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAT                                               <  SRR4302536.4338179/94‑1 (MQ=60)

                                                 GGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTTCGTATTCCACAAACAAATAATCAATATT                                              >  SRR4302536.4835880/1‑94 (MQ=60)

                                                 GGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATT                                              <  SRR4302536.1972502/94‑1 (MQ=60)

                                                  GGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTG                                             <  SRR4302536.6614258/94‑1 (MQ=60)

                                                  GGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTG                                             <  SRR4302536.5398590/94‑1 (MQ=60)

                                                  GGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTG                                             <  SRR4302536.6614259/94‑1 (MQ=60)

                                                    CACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGT                                           <  SRR4302536.3949585/94‑1 (MQ=60)

                                                    CACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGT                                           >  SRR4302536.477039/1‑94 (MQ=60)

                                                    CACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGT                                           >  SRR4302536.2099246/1‑94 (MQ=60)

                                                    CACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGT                                           >  SRR4302536.1574150/1‑94 (MQ=60)

                                                        ATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAT                                       <  SRR4302536.2255332/94‑1 (MQ=60)

                                                        ATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAT                                       >  SRR4302536.3482062/1‑94 (MQ=60)

                                                        ATGGAACACTCCCTCGAAGCCTGCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAT                                       <  SRR4302536.5604563/94‑1 (MQ=60)

                                                         TGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATT                                      <  SRR4302536.6673757/94‑1 (MQ=60)

                                                         TGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATT                                      <  SRR4302536.3620401/94‑1 (MQ=60)

                                                          GGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACACATAATCAATATTGGTGAATTA                                     <  SRR4302536.3898651/94‑1 (MQ=60)

                                                          GGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTA                                     <  SRR4302536.264780/94‑1 (MQ=60)

                                                             ACACTTCCTCGAAGCGTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAA                                  <  SRR4302536.5116200/94‑1 (MQ=60)

                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                 <  SRR4302536.5023768/94‑1 (MQ=60)

                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                 <  SRR4302536.3524187/94‑1 (MQ=60)

                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                 <  SRR4302536.2954542/94‑1 (MQ=60)

                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                 <  SRR4302536.194811/94‑1 (MQ=60)

                                                              CACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAG                                 <  SRR4302536.5018696/94‑1 (MQ=60)

                                                               CCTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGC                                <  SRR4302536.4813185/94‑1 (MQ=60)

                                                               CCTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGC                                <  SRR4302536.4992181/94‑1 (MQ=60)

                                                                CTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCG                               <  SRR4302536.2934650/94‑1 (MQ=60)

                                                                CTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCG                               <  SRR4302536.838829/94‑1 (MQ=60)

                                                                  tcataGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTA                             <  SRR4302536.6010188/89‑1 (MQ=60)

                                                                  TCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTA                             <  SRR4302536.2485292/94‑1 (MQ=60)

                                                                  TCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTA                             >  SRR4302536.2406458/1‑94 (MQ=60)

                                                                   CCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAC                            <  SRR4302536.2939334/94‑1 (MQ=60)

                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                          <  SRR4302536.2719284/94‑1 (MQ=60)

                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                          <  SRR4302536.829150/94‑1 (MQ=60)

                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                          <  SRR4302536.5359987/94‑1 (MQ=60)

                                                                     TCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCT                          <  SRR4302536.4839541/94‑1 (MQ=60)

                                                                      TGAAGCCTTCCGATATGCCGACGCTGACGGAGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                         <  SRR4302536.6353187/94‑1 (MQ=60)

                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                         <  SRR4302536.2471685/94‑1 (MQ=60)

                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                         <  SRR4302536.2433146/94‑1 (MQ=60)

                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                         <  SRR4302536.2644072/94‑1 (MQ=60)

                                                                      CGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTG                         <  SRR4302536.1104689/94‑1 (MQ=60)

                                                                       GAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGG                        >  SRR4302536.4161900/1‑94 (MQ=60)

                                                                       GAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGG                        >  SRR4302536.4082926/1‑94 (MQ=60)

                                                                        AAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGC                       >  SRR4302536.3022639/1‑94 (MQ=60)

                                                                         AGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCA                      >  SRR4302536.2516800/1‑94 (MQ=60)

                                                                          GCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAA                     >  SRR4302536.762037/1‑94 (MQ=60)

                                                                           CCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAC                    >  SRR4302536.5056198/1‑94 (MQ=60)

                                                                             TTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACAC                  <  SRR4302536.139275/94‑1 (MQ=60)

                                                                             TTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACAC                  >  SRR4302536.5038551/1‑94 (MQ=60)

                                                                                CGCGATGCCGACGCTGACGGTGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG               >  SRR4302536.6759974/1‑94 (MQ=60)

                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG               <  SRR4302536.1036606/94‑1 (MQ=60)

                                                                                CGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGG               >  SRR4302536.5049110/1‑94 (MQ=60)

                                                                                 GCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGG              <  SRR4302536.2330988/94‑1 (MQ=60)

                                                                                 CCGATGCCGACGCTGACGGCGCGCGGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGG              <  SRR4302536.1418251/94‑1 (MQ=60)

                                                                                  CGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGC             >  SRR4302536.126324/1‑94 (MQ=60)

                                                                                   GATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCG            <  SRR4302536.1461639/94‑1 (MQ=60)

                                                                                     TGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTG          <  SRR4302536.1155026/94‑1 (MQ=60)

                                                                                     TGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGCTGAATTAAAAGCGTACCTGGCAACACAGCGCGTG          >  SRR4302536.1911303/1‑94 (MQ=60)

                                                                                      GCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGG         <  SRR4302536.5404355/94‑1 (MQ=60)

                                                                                       CCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGA        >  SRR4302536.166081/1‑94 (MQ=60)

                                                                                        CGACGCTGACGGCGCGCTGGCAGCTTCCGTATTTCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAG       >  SRR4302536.2975640/1‑94 (MQ=60)

                                                                                        CGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAG       <  SRR4302536.4664641/94‑1 (MQ=60)

                                                                                        CGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAATGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAG       <  SRR4302536.2827844/94‑1 (MQ=60)

                                                                                           CGCTGAGGGCGCGCTGGCAGCTTCAGTATTCCACAAACAAATAATCAATATCGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATC    <  SRR4302536.2697723/94‑1 (MQ=60)

                                                                                            GCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCA   <  SRR4302536.2543614/94‑1 (MQ=60)

                                                                                             CTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAG  <  SRR4302536.3260545/94‑1 (MQ=60)

AAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAG  >  NC_000913/2094446‑2094632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`