Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1357 179.3 9500450 96.1% 9129932 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC99.1% 744.6 / ‑3.4 230T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (127/100);  total (128/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 239 total aligned reads displayed.

CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163
                                                                                          |                                                                                         
cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaa                                                                                         >  1:2315429/1‑93 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAc                                                                                       >  1:304160/1‑93 (MQ=255)
   aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      >  1:3496718/1‑93 (MQ=255)
     cTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGc                                                                                    <  2:3114309/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:3173410/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  2:2155420/1‑93 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:27918/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  2:1173472/1‑93 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  <  2:1677517/93‑1 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  2:48031/93‑1 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 >  1:1913820/1‑93 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 >  1:343132/1‑93 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  2:2908949/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  1:1524193/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             <  1:3766978/93‑1 (MQ=255)
               cGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGCCGGCAAACAGCATCGGGatat                                                                          <  1:1257231/93‑1 (MQ=255)
                gTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtt                                                                         >  1:2109809/1‑93 (MQ=255)
                gTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtt                                                                         >  2:130395/1‑93 (MQ=255)
                  tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       <  1:57436/93‑1 (MQ=255)
                  tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       <  2:596681/93‑1 (MQ=255)
                  tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       >  2:2782352/1‑93 (MQ=255)
                   aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTg                                                                      >  2:2529477/1‑93 (MQ=255)
                    aaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGc                                                                     >  2:3614520/1‑93 (MQ=255)
                     aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  1:2836435/93‑1 (MQ=255)
                      gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  1:276517/93‑1 (MQ=255)
                      gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  1:3008849/93‑1 (MQ=255)
                      gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  1:3707271/93‑1 (MQ=255)
                       tACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTa                                                                  <  2:4299104/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  2:4428979/93‑1 (MQ=255)
                         cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                <  2:2396735/93‑1 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               >  1:2630928/1‑93 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               >  1:4253765/1‑93 (MQ=255)
                           ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              <  1:2481035/93‑1 (MQ=255)
                            cACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgc                                                             >  2:964181/1‑93 (MQ=255)
                             aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  1:4117002/1‑93 (MQ=255)
                             aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  1:1992800/1‑93 (MQ=255)
                             aCGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgcg                                                            >  1:1615614/1‑93 (MQ=255)
                             aCGCGCATCGCCAGCGAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgac                                                            >  2:4018240/1‑91 (MQ=255)
                                 gcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCtt                                                        >  1:3826222/1‑93 (MQ=255)
                                 gcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCtt                                                        >  2:4132500/1‑93 (MQ=255)
                                  cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       >  2:2636442/1‑93 (MQ=255)
                                  cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTGTGCTTAATCGCGCCTTc                                                       <  2:3327282/93‑1 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  1:4376655/1‑93 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      <  1:306394/93‑1 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:879804/1‑93 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:3021968/1‑93 (MQ=255)
                                     cGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTg                                                    <  1:1883421/93‑1 (MQ=255)
                                     cGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTg                                                    >  2:2851464/1‑93 (MQ=255)
                                      gCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGt                                                   >  1:4240298/1‑93 (MQ=255)
                                       ccAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTa                                                  >  1:1052328/1‑93 (MQ=255)
                                        cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 >  1:3267593/1‑93 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               <  1:4705194/93‑1 (MQ=255)
                                            taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             <  2:1896237/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  1:1689878/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:529120/1‑93 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:4573193/1‑93 (MQ=255)
                                               gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:2324276/93‑1 (MQ=255)
                                               gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:2131546/93‑1 (MQ=255)
                                                 gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  2:2463337/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  1:4315646/1‑93 (MQ=255)
                                                     gCAAAAAGTTTAATGCCTTCAGCGTCAGTGGAGCCGGTAAACAGGATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  1:737601/93‑1 (MQ=255)
                                                      cAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAc                                   <  2:1810407/93‑1 (MQ=255)
                                                      cAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAc                                   <  2:2268982/93‑1 (MQ=255)
                                                       aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACg                                  >  2:3993136/1‑93 (MQ=255)
                                                       aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTATTCGCGCCTTCCTGTAACAGCGCAGCGAAACg                                  >  2:3825960/1‑93 (MQ=255)
                                                       aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACg                                  >  1:1358751/1‑93 (MQ=255)
                                                        aaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGt                                 >  1:3175189/1‑93 (MQ=255)
                                                         aaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGtt                                >  2:804715/1‑93 (MQ=255)
                                                         aaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGtt                                >  2:3699571/1‑93 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:333996/93‑1 (MQ=255)
                                                           aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              <  2:2059605/93‑1 (MQ=255)
                                                           aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              >  1:4066130/1‑93 (MQ=255)
                                                           aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              <  2:2695057/93‑1 (MQ=255)
                                                            gTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTg                             >  1:878589/1‑93 (MQ=255)
                                                            gTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTg                             <  2:1725888/93‑1 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            <  1:607332/93‑1 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            >  1:3554924/1‑93 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        >  2:4183531/1‑93 (MQ=255)
                                                                  tCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGtt                       >  2:1012744/1‑93 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     >  1:515459/1‑93 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     <  1:4346211/93‑1 (MQ=255)
                                                                    ccTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     <  2:1131601/92‑1 (MQ=255)
                                                                     gttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  1:2044901/2‑93 (MQ=255)
                                                                     cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  1:1146662/1‑93 (MQ=255)
                                                                     cTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                    >  1:4207228/1‑93 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:1588703/1‑93 (MQ=255)
                                                                        cTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAc                 <  2:138120/93‑1 (MQ=255)
                                                                          gCTTCAGTGGAGTCGGCAAATAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCTAAACGTTCTGCTCGTTCTGAACGc               <  2:3968491/93‑1 (MQ=255)
                                                                            ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCAAAACGTTCTGCGCGTTCTGAACGCTc             <  2:4350589/93‑1 (MQ=255)
                                                                               aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          <  2:4496117/93‑1 (MQ=255)
                                                                               aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          >  1:2350480/1‑93 (MQ=255)
                                                                                gTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCg         >  2:699794/1‑93 (MQ=255)
                                                                                gTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCg         >  1:1423828/1‑93 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGag       >  1:2894309/1‑92 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       <  1:3755910/93‑1 (MQ=255)
                                                                                    aGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGa     <  1:4586316/93‑1 (MQ=255)
                                                                                     gTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGAc    >  2:3808963/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  1:225610/1‑93 (MQ=255)
                                                                                       cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:1102485/93‑1 (MQ=255)
                                                                                       cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:2678998/93‑1 (MQ=255)
                                                                                          |                                                                                         
CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCCAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGC                                                                                               >  SRR4302544.1086375/1‑94 (MQ=60)
 TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                              >  SRR4302544.176109/1‑94 (MQ=60)
  GCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAA                                                                                             >  SRR4302544.2337344/1‑94 (MQ=60)
  GCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTGTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAA                                                                                             >  SRR4302544.2337345/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           <  SRR4302544.4653218/94‑1 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302544.307314/1‑94 (MQ=60)
    ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGCGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                           >  SRR4302544.3367228/1‑94 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          <  SRR4302544.595084/94‑1 (MQ=60)
     TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                          >  SRR4302544.3530060/1‑94 (MQ=60)
         TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                      >  SRR4302544.1210393/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302544.363054/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302544.1932313/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302544.1582530/1‑94 (MQ=60)
          ATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATC                                                                                     >  SRR4302544.346675/1‑94 (MQ=60)
           TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    <  SRR4302544.2195538/94‑1 (MQ=60)
           TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                    >  SRR4302544.1539318/1‑94 (MQ=60)
             CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                  <  SRR4302544.4387282/94‑1 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                <  SRR4302544.3804335/94‑1 (MQ=60)
               GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                                >  SRR4302544.1518602/1‑94 (MQ=60)
                TTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATA                                                                               >  SRR4302544.37974/1‑94 (MQ=60)
                 TCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAT                                                                              >  SRR4302544.311671/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             <  SRR4302544.53387/94‑1 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302544.2130099/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                             >  SRR4302544.2086021/1‑94 (MQ=60)
                  CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGCCGGCAAACAGCATCGGGATATT                                                                             <  SRR4302544.1269603/94‑1 (MQ=60)
                     TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                          <  SRR4302544.58011/94‑1 (MQ=60)
                      AAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGC                                                                         >  SRR4302544.871548/1‑94 (MQ=60)
                        AGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTT                                                                       <  SRR4302544.2863833/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302544.3744159/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302544.279413/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302544.3037691/94‑1 (MQ=60)
                         GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                      <  SRR4302544.4102472/94‑1 (MQ=60)
                          TACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAA                                                                     >  SRR4302544.4272172/1‑94 (MQ=60)
                          TACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAA                                                                     >  SRR4302544.3799073/1‑94 (MQ=60)
                          TACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAA                                                                     <  SRR4302544.2019934/94‑1 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302544.4297134/1‑94 (MQ=60)
                            CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                   >  SRR4302544.2656149/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                  >  SRR4302544.1932253/1‑94 (MQ=60)
                             GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGCGGAGTCGGTAAACAGCATCGGGAGATTTTGCTTAATtc                                                                  >  SRR4302544.2288644/1‑92 (MQ=60)
                              CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                 <  SRR4302544.2504787/94‑1 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302544.1631541/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302544.3662889/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302544.2012147/1‑94 (MQ=60)
                               CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                                >  SRR4302544.4157885/1‑94 (MQ=60)
                                 CGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCC                                                              >  SRR4302544.54584/1‑94 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             >  SRR4302544.3574063/1‑94 (MQ=60)
                                  GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                             <  SRR4302544.4300235/94‑1 (MQ=60)
                                   CGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTT                                                            >  SRR4302544.3864166/1‑94 (MQ=60)
                                    GCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTC                                                           <  SRR4302544.2382203/94‑1 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          >  SRR4302544.4421013/1‑94 (MQ=60)
                                     CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                          <  SRR4302544.2969572/94‑1 (MQ=60)
                                      ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                         <  SRR4302544.309568/94‑1 (MQ=60)
                                       TCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTG                                                        <  SRR4302544.1737262/94‑1 (MQ=60)
                                        CGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGT                                                       <  SRR4302544.1901627/94‑1 (MQ=60)
                                        CGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGT                                                       >  SRR4302544.4283514/1‑94 (MQ=60)
                                        CGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGT                                                       >  SRR4302544.4283516/1‑94 (MQ=60)
                                         GCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTA                                                      >  SRR4302544.1062762/1‑94 (MQ=60)
                                          CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     >  SRR4302544.3298776/1‑94 (MQ=60)
                                          CCAGGCAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                     >  SRR4302544.490489/1‑94 (MQ=60)
                                           CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                    <  SRR4302544.3465482/94‑1 (MQ=60)
                                            AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                   >  SRR4302544.1328957/1‑94 (MQ=60)
                                             GGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAG                                                  <  SRR4302544.4751689/94‑1 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                <  SRR4302544.423272/94‑1 (MQ=60)
                                               TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                                <  SRR4302544.4361507/94‑1 (MQ=60)
                                                AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                               >  SRR4302544.1622474/1‑94 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              >  SRR4302544.2844543/1‑94 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              <  SRR4302544.2049747/94‑1 (MQ=60)
                                                 GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                              <  SRR4302544.1706545/94‑1 (MQ=60)
                                                  GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                             <  SRR4302544.4107072/94‑1 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302544.4359611/1‑94 (MQ=60)
                                                    GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                           >  SRR4302544.3365644/1‑94 (MQ=60)
                                                        TCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       <  SRR4302544.1336283/94‑1 (MQ=60)
                                                        GCAAAAAGTTTAATGCCTTCAGCGTCAGTGGAGCCGGTAAACAGGATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       <  SRR4302544.744817/94‑1 (MQ=60)
                                                        GCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAC                                       >  SRR4302544.3085518/1‑94 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                      >  SRR4302544.1372239/1‑94 (MQ=60)
                                                         CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                      >  SRR4302544.1372240/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302544.4290658/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302544.3205404/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302544.3040542/1‑94 (MQ=60)
                                                          AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                     >  SRR4302544.1839994/1‑94 (MQ=60)
                                                           AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                    >  SRR4302544.2761889/1‑94 (MQ=60)
                                                            AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                   <  SRR4302544.2549959/94‑1 (MQ=60)
                                                             AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                  >  SRR4302544.4106617/1‑94 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 >  SRR4302544.887301/1‑94 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 <  SRR4302544.3268765/94‑1 (MQ=60)
                                                              AGTTTAATCGCTTCTGCTGCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                 <  SRR4302544.2394299/94‑1 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                >  SRR4302544.3588990/1‑94 (MQ=60)
                                                               GTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGC                                <  SRR4302544.3184536/94‑1 (MQ=60)
                                                                TTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCG                               <  SRR4302544.613293/94‑1 (MQ=60)
                                                                    ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                           >  SRR4302544.1151310/1‑94 (MQ=60)
                                                                      CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                         >  SRR4302544.520555/1‑94 (MQ=60)
                                                                       GGTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302544.2064612/1‑94 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        <  SRR4302544.4390392/94‑1 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302544.1158067/1‑94 (MQ=60)
                                                                       GCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTG                        >  SRR4302544.4249471/1‑94 (MQ=60)
                                                                        CTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGA                       <  SRR4302544.3769618/94‑1 (MQ=60)
                                                                           CTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTGGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACG                    <  SRR4302544.2916296/94‑1 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302544.3001663/1‑94 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302544.620501/1‑94 (MQ=60)
                                                                            TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                   >  SRR4302544.992369/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCA                >  SRR4302544.4440829/1‑94 (MQ=60)
                                                                               TTCAGTGGAGTCGG‑‑CACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACC              <  SRR4302544.4134591/94‑1 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               >  SRR4302544.4409177/1‑94 (MQ=60)
                                                                                TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC               >  SRR4302544.3813477/1‑94 (MQ=60)
                                                                                 CAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACC              >  SRR4302544.2372747/1‑94 (MQ=60)
                                                                                  AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG             >  SRR4302544.1437998/1‑94 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT           >  SRR4302544.793863/1‑94 (MQ=60)
                                                                                    TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAG           >  SRR4302544.2922185/1‑94 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302544.3793193/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302544.4634107/94‑1 (MQ=60)
                                                                                     GGAGTCGGCAAACAGCAGCGGGATATTTTGCTTAAGCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG          <  SRR4302544.2762695/94‑1 (MQ=60)
                                                                                      GAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGA         <  SRR4302544.1937690/94‑1 (MQ=60)
                                                                                      GAGTCGACAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGA         <  SRR4302544.1658878/94‑1 (MQ=60)
                                                                                       AGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGAC        <  SRR4302544.4631974/94‑1 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAgaacgtttc       >  SRR4302544.517115/1‑85 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       >  SRR4302544.227952/1‑94 (MQ=60)
                                                                                        GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA       <  SRR4302544.2202954/94‑1 (MQ=60)
                                                                                           GGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATA    >  SRR4302544.4210148/1‑94 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   <  SRR4302544.1603641/94‑1 (MQ=60)
                                                                                            GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCAGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC   <  SRR4302544.624626/94‑1 (MQ=60)
                                                                                             CAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  SRR4302544.3662395/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACG  >  NC_000913/2096981‑2097167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑