Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC99.6% 800.9 / ‑5.7 244T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (122/121);  total (123/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 245 total aligned reads displayed.

ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096985‑2097163
                                                                                         |                                                                                         
aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        >  1:5754579/1‑93 (MQ=255)
 tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAc                                                                                       >  1:3657489/1‑93 (MQ=255)
  aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      >  2:3687475/1‑93 (MQ=255)
   gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     >  1:760509/1‑93 (MQ=255)
     tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAATTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  1:957778/93‑1 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  1:4775904/1‑93 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  2:6271663/1‑93 (MQ=255)
      aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  <  2:464401/93‑1 (MQ=255)
       tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:1015296/93‑1 (MQ=255)
       tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:4032542/93‑1 (MQ=255)
       tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  2:3964550/93‑1 (MQ=255)
        ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                <  1:5919004/93‑1 (MQ=255)
         cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               <  2:1429836/93‑1 (MQ=255)
         cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               <  1:457170/93‑1 (MQ=255)
         cAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCgg                                                                               <  2:4145814/93‑1 (MQ=255)
          aGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCggg                                                                              >  1:76550/1‑93 (MQ=255)
           gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  2:5683878/1‑93 (MQ=255)
           gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  2:4434997/1‑93 (MQ=255)
           gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             <  2:3190845/93‑1 (MQ=255)
            ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  2:3747015/1‑93 (MQ=255)
             tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGata                                                                           <  1:4674306/93‑1 (MQ=255)
             tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGata                                                                           <  1:6101435/93‑1 (MQ=255)
             tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGata                                                                           <  1:5386414/93‑1 (MQ=255)
               gTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtt                                                                         >  1:2403020/1‑93 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        <  1:2394822/93‑1 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        >  1:4841356/1‑93 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        >  1:1730856/1‑93 (MQ=255)
                ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        <  1:2123305/93‑1 (MQ=255)
                 tAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCTCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAtttt                                                                       <  2:1795663/93‑1 (MQ=255)
                  aaaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCAGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTg                                                                      >  1:5557738/1‑93 (MQ=255)
                   aaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGc                                                                     <  2:750638/93‑1 (MQ=255)
                   aaGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGc                                                                     >  2:5905663/1‑93 (MQ=255)
                    aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    >  1:1502833/1‑93 (MQ=255)
                    aGTACGCCACGCGCATCGCCAGGTAGATGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  1:6426174/93‑1 (MQ=255)
                     gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  1:2559401/93‑1 (MQ=255)
                     gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   >  2:2608116/1‑93 (MQ=255)
                        cGCCACGTGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAATCTGCATCGGGATATTTTGCTTAAt                                                                <  1:3550365/93‑1 (MQ=255)
                        cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                <  2:264357/93‑1 (MQ=255)
                        cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                >  1:4393094/1‑93 (MQ=255)
                        cGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAt                                                                <  1:80074/93‑1 (MQ=255)
                         cccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:1416627/92‑1 (MQ=255)
                           cACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcgc                                                             >  1:1321320/1‑93 (MQ=255)
                              gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          >  1:2704730/1‑93 (MQ=255)
                              gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          >  2:6514982/1‑93 (MQ=255)
                               cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         <  2:299931/93‑1 (MQ=255)
                                gcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCtt                                                        >  1:182650/1‑93 (MQ=255)
                                 cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       >  2:3169359/1‑93 (MQ=255)
                                 cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       <  1:6700280/93‑1 (MQ=255)
                                  aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      <  2:4379874/93‑1 (MQ=255)
                                  aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  2:897186/1‑93 (MQ=255)
                                  aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      >  1:4987638/1‑93 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     >  1:6368794/1‑93 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     >  2:6396148/1‑93 (MQ=255)
                                   tCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCt                                                     <  1:2133618/93‑1 (MQ=255)
                                      ccAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTa                                                  <  1:938676/93‑1 (MQ=255)
                                       cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 <  2:2474934/93‑1 (MQ=255)
                                        aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                <  1:1248524/93‑1 (MQ=255)
                                        aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  1:5414822/1‑93 (MQ=255)
                                        aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  2:234308/1‑93 (MQ=255)
                                            aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  2:2839931/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:157061/93‑1 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:1892561/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:1344850/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:1266998/1‑93 (MQ=255)
                                             ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           >  2:4877611/1‑93 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          >  1:198414/1‑93 (MQ=255)
                                              gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagca                                          <  2:5814106/93‑1 (MQ=255)
                                               tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  1:4182068/1‑93 (MQ=255)
                                               tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  2:1996017/1‑93 (MQ=255)
                                               tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  2:4773603/1‑93 (MQ=255)
                                                gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  1:3074470/1‑93 (MQ=255)
                                                 tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  1:4016875/1‑93 (MQ=255)
                                                 tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:1143486/1‑93 (MQ=255)
                                                 tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       <  1:6266269/93‑1 (MQ=255)
                                                      aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACg                                  >  1:1534007/1‑93 (MQ=255)
                                                      aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACg                                  <  2:1749234/93‑1 (MQ=255)
                                                      aaaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACg                                  >  2:1737328/1‑93 (MQ=255)
                                                         aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:3899191/93‑1 (MQ=255)
                                                         aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  1:5510929/93‑1 (MQ=255)
                                                         aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  1:6190385/93‑1 (MQ=255)
                                                          aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              >  1:3618153/1‑93 (MQ=255)
                                                          aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              <  2:4543839/93‑1 (MQ=255)
                                                          aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              <  2:1072370/93‑1 (MQ=255)
                                                             ttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcg                           >  2:3468490/1‑93 (MQ=255)
                                                                 tCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGtt                       >  1:4502208/1‑93 (MQ=255)
                                                                  cGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTc                      <  1:4897241/93‑1 (MQ=255)
                                                                  cGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTc                      <  1:2876906/93‑1 (MQ=255)
                                                                   gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     >  1:5115043/1‑93 (MQ=255)
                                                                   gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     <  2:5472678/93‑1 (MQ=255)
                                                                     ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:2748331/1‑93 (MQ=255)
                                                                       cTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAAc                 <  2:4662991/93‑1 (MQ=255)
                                                                        tGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACg                >  2:6327260/1‑93 (MQ=255)
                                                                         gCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGc               <  2:5373148/93‑1 (MQ=255)
                                                                           ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             >  1:5097619/1‑93 (MQ=255)
                                                                              aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          <  1:6615911/93‑1 (MQ=255)
                                                                                 ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       >  2:6191267/1‑93 (MQ=255)
                                                                                 ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       >  1:3792064/1‑93 (MQ=255)
                                                                                  gAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATg      >  1:3385694/1‑93 (MQ=255)
                                                                                      cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:6626134/93‑1 (MQ=255)
                                                                                      cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  >  1:1456367/1‑93 (MQ=255)
                                                                                         |                                                                                         
ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096985‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC  >  NC_000913/2096982‑2097166
                                                                                            |                                                                                            
TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCA                                                                                             >  SRR4302536.4752799/1‑94 (MQ=60)
  gctagccATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAGCGCTTCTGCTTCAGTGGAGTCAGCAAA                                                                                           <  SRR4302536.5685445/87‑1 (MQ=60)
  CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAA                                                                                           >  SRR4302536.5925746/1‑94 (MQ=60)
  CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAA                                                                                           >  SRR4302536.149732/1‑94 (MQ=60)
   ATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAAC                                                                                          >  SRR4302536.3766828/1‑94 (MQ=60)
    TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                         <  SRR4302536.246205/94‑1 (MQ=60)
    TAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACA                                                                                         >  SRR4302536.2649450/1‑94 (MQ=60)
     AGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                        <  SRR4302536.1493213/94‑1 (MQ=60)
     AGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                        >  SRR4302536.4998436/1‑94 (MQ=60)
     AGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAG                                                                                        >  SRR4302536.782806/1‑94 (MQ=60)
      GCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGC                                                                                       <  SRR4302536.1518573/94‑1 (MQ=60)
       CTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCA                                                                                      >  SRR4302536.51565/1‑94 (MQ=60)
        TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAATTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                     <  SRR4302536.986085/94‑1 (MQ=60)
        TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                     >  SRR4302536.4919700/1‑94 (MQ=60)
        TATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAT                                                                                     <  SRR4302536.1233039/94‑1 (MQ=60)
          TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                   <  SRR4302536.1045309/94‑1 (MQ=60)
          TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                   <  SRR4302536.5620169/94‑1 (MQ=60)
          TCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCG                                                                                   <  SRR4302536.4152144/94‑1 (MQ=60)
           CCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGG                                                                                  <  SRR4302536.6095311/94‑1 (MQ=60)
            CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                 >  SRR4302536.78604/1‑94 (MQ=60)
            CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                 <  SRR4302536.922519/94‑1 (MQ=60)
            CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                 <  SRR4302536.469148/94‑1 (MQ=60)
            CAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGG                                                                                 <  SRR4302536.2958759/94‑1 (MQ=60)
             AGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGA                                                                                >  SRR4302536.3721632/1‑94 (MQ=60)
              GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                               <  SRR4302536.4619944/94‑1 (MQ=60)
              GTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGAT                                                                               <  SRR4302536.2408856/94‑1 (MQ=60)
                TCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAT                                                                             <  SRR4302536.6283344/94‑1 (MQ=60)
                TCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAT                                                                             <  SRR4302536.5547179/94‑1 (MQ=60)
                TCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAT                                                                             <  SRR4302536.4814869/94‑1 (MQ=60)
                 CGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATT                                                                            >  SRR4302536.2475735/1‑94 (MQ=60)
                  GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                           >  SRR4302536.1780813/1‑94 (MQ=60)
                  GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                           <  SRR4302536.5519769/94‑1 (MQ=60)
                  GTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTT                                                                           >  SRR4302536.4987065/1‑94 (MQ=60)
                   TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                          <  SRR4302536.2187102/94‑1 (MQ=60)
                   TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                          <  SRR4302536.1977750/94‑1 (MQ=60)
                   TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                          <  SRR4302536.2467317/94‑1 (MQ=60)
                   TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                          <  SRR4302536.2043603/94‑1 (MQ=60)
                   TTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTT                                                                          <  SRR4302536.1371432/94‑1 (MQ=60)
                    TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                         <  SRR4302536.6586645/94‑1 (MQ=60)
                    TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                         <  SRR4302536.4671235/94‑1 (MQ=60)
                    TAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCAGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTG                                                                         >  SRR4302536.5723261/1‑94 (MQ=60)
                      AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                       >  SRR4302536.1547147/1‑94 (MQ=60)
                      AAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCT                                                                       <  SRR4302536.4724505/94‑1 (MQ=60)
                       AGTACGCCACGCGCATCGCCAGGTAGATGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTT                                                                      <  SRR4302536.6618245/94‑1 (MQ=60)
                        GTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTA                                                                     <  SRR4302536.2636429/94‑1 (MQ=60)
                          ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                   >  SRR4302536.1567621/1‑94 (MQ=60)
                          ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                   >  SRR4302536.6709909/1‑94 (MQ=60)
                          ACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAAT                                                                   >  SRR4302536.4524323/1‑94 (MQ=60)
                           CGCCACGTGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAATCTGCATCGGGATATTTTGCTTAATC                                                                  <  SRR4302536.3656518/94‑1 (MQ=60)
                           CGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATC                                                                  <  SRR4302536.82226/94‑1 (MQ=60)
                            GCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                 <  SRR4302536.2998040/94‑1 (MQ=60)
                            CCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCG                                                                 <  SRR4302536.1458735/94‑1 (MQ=60)
                             CCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGC                                                                >  SRR4302536.1360765/1‑94 (MQ=60)
                              CACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCG                                                               >  SRR4302536.4550696/1‑94 (MQ=60)
                                CGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCC                                                             >  SRR4302536.2785400/1‑94 (MQ=60)
                                 GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                            <  SRR4302536.2516831/94‑1 (MQ=60)
                                 GCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCT                                                            <  SRR4302536.6424373/94‑1 (MQ=60)
                                  CTCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTT                                                           <  SRR4302536.4333574/94‑1 (MQ=60)
                                  CGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTT                                                           >  SRR4302536.187481/1‑94 (MQ=60)
                                    CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                         >  SRR4302536.5137368/1‑94 (MQ=60)
                                    CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                         <  SRR4302536.6899291/94‑1 (MQ=60)
                                    CATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCC                                                         <  SRR4302536.6899292/94‑1 (MQ=60)
                                     ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                        >  SRR4302536.6558582/1‑94 (MQ=60)
                                     ATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCT                                                        <  SRR4302536.6469671/94‑1 (MQ=60)
                                      TCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTG                                                       <  SRR4302536.2197652/94‑1 (MQ=60)
                                        GCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTA                                                     <  SRR4302536.5029631/94‑1 (MQ=60)
                                         CCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAA                                                    <  SRR4302536.966353/94‑1 (MQ=60)
                                          CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                   <  SRR4302536.974251/94‑1 (MQ=60)
                                          CAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAC                                                   >  SRR4302536.5576649/1‑94 (MQ=60)
                                           AGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACA                                                  <  SRR4302536.1286007/94‑1 (MQ=60)
                                             GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                >  SRR4302536.6151248/1‑94 (MQ=60)
                                             GTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGA                                                >  SRR4302536.5155505/1‑94 (MQ=60)
                                              TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                               <  SRR4302536.2515802/94‑1 (MQ=60)
                                              TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                               <  SRR4302536.5773807/94‑1 (MQ=60)
                                              TAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAG                                               >  SRR4302536.3736514/1‑94 (MQ=60)
                                               AGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGC                                              >  SRR4302536.4025480/1‑94 (MQ=60)
                                                GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                             <  SRR4302536.4140443/94‑1 (MQ=60)
                                                GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                             >  SRR4302536.203631/1‑94 (MQ=60)
                                                GGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCA                                             <  SRR4302536.4878860/94‑1 (MQ=60)
                                                 GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                            <  SRR4302536.5049751/94‑1 (MQ=60)
                                                 GTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAG                                            >  SRR4302536.4306303/1‑94 (MQ=60)
                                                  TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                           >  SRR4302536.3164513/1‑94 (MQ=60)
                                                  TGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGC                                           >  SRR4302536.4301780/1‑94 (MQ=60)
                                                   GTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCG                                          >  SRR4302536.4136058/1‑94 (MQ=60)
                                                    TTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGA                                         >  SRR4302536.2955983/1‑94 (MQ=60)
                                                    TTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGA                                         <  SRR4302536.6452953/94‑1 (MQ=60)
                                                      TGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGTAA                                       <  SRR4302536.3573918/94‑1 (MQ=60)
                                                      TGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAA                                       >  SRR4302536.5253218/1‑94 (MQ=60)
                                                        CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                     <  SRR4302536.4212164/94‑1 (MQ=60)
                                                        CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                     <  SRR4302536.75797/94‑1 (MQ=60)
                                                        CAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACG                                     >  SRR4302536.1579135/1‑94 (MQ=60)
                                                         AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGTATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                    <  SRR4302536.2192503/94‑1 (MQ=60)
                                                         AAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGT                                    >  SRR4302536.3185832/1‑94 (MQ=60)
                                                          AAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTT                                   <  SRR4302536.1348505/94‑1 (MQ=60)
                                                           AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                  >  SRR4302536.5570067/1‑94 (MQ=60)
                                                           AAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                  <  SRR4302536.5346325/94‑1 (MQ=60)
                                                           AAAGTTGAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTC                                  <  SRR4302536.3703006/94‑1 (MQ=60)
                                                            AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                 <  SRR4302536.2150890/94‑1 (MQ=60)
                                                            AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                 <  SRR4302536.6374782/94‑1 (MQ=60)
                                                            AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                 <  SRR4302536.5675305/94‑1 (MQ=60)
                                                            AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                 >  SRR4302536.3726322/1‑94 (MQ=60)
                                                            AAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCT                                 <  SRR4302536.4109835/94‑1 (MQ=60)
                                                             AGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTG                                >  SRR4302536.3896775/1‑94 (MQ=60)
                                                                  AATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGT                           <  SRR4302536.6319962/94‑1 (MQ=60)
                                                                   ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                          <  SRR4302536.2319994/94‑1 (MQ=60)
                                                                   ATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTT                          >  SRR4302536.4637325/1‑94 (MQ=60)
                                                                    TCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTC                         >  SRR4302536.4794029/1‑94 (MQ=60)
                                                                     CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                        <  SRR4302536.5044526/94‑1 (MQ=60)
                                                                     CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                        <  SRR4302536.5628989/94‑1 (MQ=60)
                                                                     CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                        >  SRR4302536.5267904/1‑94 (MQ=60)
                                                                     CGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCT                        <  SRR4302536.2961831/94‑1 (MQ=60)
                                                                        TTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAA                     >  SRR4302536.5977350/1‑94 (MQ=60)
                                                                           TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                  >  SRR4302536.935262/1‑94 (MQ=60)
                                                                           TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                  >  SRR4302536.820190/1‑94 (MQ=60)
                                                                           TGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGC                  >  SRR4302536.1647897/1‑94 (MQ=60)
                                                                            GCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                 <  SRR4302536.573982/94‑1 (MQ=60)
                                                                            GCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCT                 <  SRR4302536.4974223/94‑1 (MQ=60)
                                                                             CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                <  SRR4302536.6291697/94‑1 (MQ=60)
                                                                             CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                >  SRR4302536.649674/1‑94 (MQ=60)
                                                                             CTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTC                >  SRR4302536.5250044/1‑94 (MQ=60)
                                                                               TCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAC              >  SRR4302536.3434094/1‑94 (MQ=60)
                                                                                 AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCTCGTTCTGAACcgtcaccc            >  SRR4302536.1625354/1‑86 (MQ=60)
                                                                                 AGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCG            <  SRR4302536.6812614/94‑1 (MQ=60)
                                                                                   TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT          >  SRR4302536.3905189/1‑94 (MQ=60)
                                                                                   TGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAT          >  SRR4302536.6899585/1‑94 (MQ=60)
                                                                                    GGAGTCGGCAAACAGTATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG         <  SRR4302536.6652239/94‑1 (MQ=60)
                                                                                    GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG         >  SRR4302536.1409844/1‑94 (MQ=60)
                                                                                    GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG         >  SRR4302536.3486326/1‑94 (MQ=60)
                                                                                    GGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATG         >  SRR4302536.783414/1‑94 (MQ=60)
                                                                                       GTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACA      >  SRR4302536.1018980/1‑94 (MQ=60)
                                                                                        TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA     >  SRR4302536.5722652/1‑94 (MQ=60)
                                                                                        TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA     >  SRR4302536.1499491/1‑94 (MQ=60)
                                                                                        TCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA     >  SRR4302536.1798279/1‑94 (MQ=60)
                                                                                         CGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAAT    >  SRR4302536.5409193/1‑94 (MQ=60)
                                                                                           GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC  >  SRR4302536.5414509/1‑94 (MQ=60)
                                                                                           GCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC  >  SRR4302536.2835366/1‑94 (MQ=60)
                                                                                            |                                                                                            
TGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATAC  >  NC_000913/2096982‑2097166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑