Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,123,880 T→C Q123R (CAG→CGG)  wcaH ← GDP‑mannose mannosyl hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,123,8800TC100.0% 670.0 / NA 205Q123R (CAG→CGG) wcaHGDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (102/103);  total (102/103)

Only 100 of 221 total aligned reads displayed.

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT  >  NC_000913/2123790‑2123970
                                                                                          |                                                                                          
ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgccgc                                                                                          >  2:1675237/1‑93 (MQ=255)
ttCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCAtgccgc                                                                                          >  1:2379799/1‑93 (MQ=255)
 tctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATgccgct                                                                                         <  1:612340/93‑1 (MQ=255)
 tctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATgccgct                                                                                         <  2:6526720/93‑1 (MQ=255)
  ctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTc                                                                                        <  1:3585441/93‑1 (MQ=255)
    cAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAt                                                                                      >  1:6419423/1‑93 (MQ=255)
     aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATc                                                                                     <  1:2893754/93‑1 (MQ=255)
     aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATc                                                                                     <  1:3730816/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                    >  2:2686235/1‑93 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                    >  1:3473047/1‑93 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                    <  1:5233982/93‑1 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                    >  1:966705/1‑93 (MQ=255)
      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                    <  2:2165352/93‑1 (MQ=255)
       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                   <  1:4138999/93‑1 (MQ=255)
       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                   <  1:2088875/93‑1 (MQ=255)
       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                   <  2:6140540/93‑1 (MQ=255)
        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgg                                                                                  >  1:1055407/1‑93 (MQ=255)
        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgg                                                                                  >  1:1808276/1‑93 (MQ=255)
        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgc                                                                                  >  2:467351/1‑92 (MQ=255)
         agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGc                                                                                 <  2:3045424/93‑1 (MQ=255)
         agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCAAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGc                                                                                 <  1:1777141/93‑1 (MQ=255)
          gaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCa                                                                                <  2:347133/93‑1 (MQ=255)
               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCTGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                           >  2:4853737/1‑93 (MQ=255)
               tAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                           >  2:795318/1‑93 (MQ=255)
                 ggCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAg                                                                         <  2:3617429/93‑1 (MQ=255)
                 ggCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAg                                                                         >  1:402150/1‑93 (MQ=255)
                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                        <  2:2045023/93‑1 (MQ=255)
                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                        <  2:4143069/93‑1 (MQ=255)
                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                    <  1:2563938/93‑1 (MQ=255)
                       gCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                   <  2:6370894/93‑1 (MQ=255)
                       gCTGTTAGCATAATCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGTGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttc                                                                   <  1:5433545/93‑1 (MQ=255)
                        cTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttct                                                                  <  1:4049334/93‑1 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTCACAGCTcttctt                                                                 >  1:1689065/1‑93 (MQ=255)
                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                 >  2:2964074/1‑93 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                                >  1:4616953/1‑93 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                                >  2:4065377/1‑93 (MQ=255)
                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                                >  1:990822/1‑93 (MQ=255)
                           ttAGCATGAACATTATCACTGGTGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                               <  1:3273184/93‑1 (MQ=255)
                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                             <  2:1051070/93‑1 (MQ=255)
                              gCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAt                                                            >  2:5762336/1‑93 (MQ=255)
                              gCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAt                                                            >  2:1469029/1‑93 (MQ=255)
                              gCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTACGAt                                                            >  2:552302/1‑93 (MQ=255)
                               cATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                           <  2:6675812/93‑1 (MQ=255)
                               cATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                           <  2:5358055/93‑1 (MQ=255)
                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                         >  1:6291811/1‑93 (MQ=255)
                                  gAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActc                                                        <  1:228737/93‑1 (MQ=255)
                                  gAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATActc                                                        <  2:5229948/93‑1 (MQ=255)
                                    aCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTg                                                      >  1:4753555/1‑93 (MQ=255)
                                     cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGa                                                     <  1:2251849/93‑1 (MQ=255)
                                     cATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGa                                                     >  2:1344503/1‑93 (MQ=255)
                                      aTTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGaa                                                    <  2:2279985/93‑1 (MQ=255)
                                       ttATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                                                   >  2:2863866/1‑93 (MQ=255)
                                        tATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                  <  1:3364253/93‑1 (MQ=255)
                                         aTCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCg                                                 >  2:1816012/1‑93 (MQ=255)
                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                                <  1:5651149/93‑1 (MQ=255)
                                           cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaa                                               <  2:4907192/93‑1 (MQ=255)
                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                              <  1:4472946/93‑1 (MQ=255)
                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                             >  1:786974/1‑93 (MQ=255)
                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                             >  2:6459170/1‑93 (MQ=255)
                                               ggCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAcc                                           >  1:5819724/1‑93 (MQ=255)
                                                gCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTACCGATACTCTGAAGCGAAAACCg                                          <  1:3124058/93‑1 (MQ=255)
                                                 cGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGa                                         <  1:6594996/93‑1 (MQ=255)
                                                 cGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGa                                         <  1:5689188/93‑1 (MQ=255)
                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                        >  1:2754846/1‑93 (MQ=255)
                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                       >  1:1959867/1‑93 (MQ=255)
                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                       >  1:4368823/1‑93 (MQ=255)
                                                   aacagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                       <  1:122335/91‑1 (MQ=255)
                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                      >  1:2112273/1‑93 (MQ=255)
                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                      >  1:3041765/1‑93 (MQ=255)
                                                    gcagcGCGCCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                      <  2:1172468/93‑1 (MQ=255)
                                                      agcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacc                                    >  1:2851241/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                   >  1:831511/1‑93 (MQ=255)
                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                   >  2:4569105/1‑93 (MQ=255)
                                                          cgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACAt                                >  1:1383415/1‑93 (MQ=255)
                                                           gTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                               <  2:3930967/93‑1 (MQ=255)
                                                           gTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATa                               <  1:3796809/93‑1 (MQ=255)
                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                              >  2:2461117/1‑93 (MQ=255)
                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                            >  2:4764783/1‑93 (MQ=255)
                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                           >  1:1552585/1‑93 (MQ=255)
                                                                 cGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagt                         <  2:4285739/93‑1 (MQ=255)
                                                                 cGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagt                         <  2:5457797/93‑1 (MQ=255)
                                                                  gTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtgagtg                        <  2:923889/93‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                       <  2:6056206/93‑1 (MQ=255)
                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                       >  2:5953464/1‑93 (MQ=255)
                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                     <  2:3214192/93‑1 (MQ=255)
                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                    <  1:4870387/93‑1 (MQ=255)
                                                                      gccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGa                    >  1:2984619/1‑93 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt             <  1:5075251/93‑1 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt             <  2:6179323/93‑1 (MQ=255)
                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt             <  1:5040815/93‑1 (MQ=255)
                                                                              tAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTg            >  1:6005704/1‑93 (MQ=255)
                                                                              tAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTg            >  2:2408973/1‑93 (MQ=255)
                                                                               aaTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGc           >  2:2637826/1‑93 (MQ=255)
                                                                               aaTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCAGTGc           >  1:654129/1‑93 (MQ=255)
                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGCAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc          >  2:1289169/1‑93 (MQ=255)
                                                                                aTCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGcc          >  2:3948515/1‑93 (MQ=255)
                                                                                   gTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCaga       <  2:1228000/93‑1 (MQ=255)
                                                                                    tCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCagag      <  1:232300/93‑1 (MQ=255)
                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga     <  2:5847971/93‑1 (MQ=255)
                                                                                       tgccgcTCATCCGGCAGT‑ACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGt  <  2:461334/93‑1 (MQ=255)
                                                                                          |                                                                                          
TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT  >  NC_000913/2123790‑2123970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123789‑2123973
                                                                                           |                                                                                             
CTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGC                                                                                             >  SRR4302536.2451893/1‑94 (MQ=60)
 TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGTGAATCGTCATGCCGCT                                                                                            >  SRR4302536.6371826/1‑94 (MQ=60)
  TCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTC                                                                                           <  SRR4302536.630620/94‑1 (MQ=60)
   CTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCA                                                                                          <  SRR4302536.3692657/94‑1 (MQ=60)
   CTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCA                                                                                          >  SRR4302536.3072513/1‑94 (MQ=60)
    TCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAT                                                                                         >  SRR4302536.6611271/1‑94 (MQ=60)
    TCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCCCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCAT                                                                                         <  SRR4302536.57486/94‑1 (MQ=60)
      AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302536.3842335/94‑1 (MQ=60)
      AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       >  SRR4302536.995249/1‑94 (MQ=60)
      AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       >  SRR4302536.3576571/1‑94 (MQ=60)
      AGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCC                                                                                       <  SRR4302536.2979089/94‑1 (MQ=60)
       GCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCG                                                                                      <  SRR4302536.5389934/94‑1 (MQ=60)
        CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302536.4261773/94‑1 (MQ=60)
        CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302536.1836833/94‑1 (MQ=60)
        CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     >  SRR4302536.1862837/1‑94 (MQ=60)
        CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     >  SRR4302536.1086956/1‑94 (MQ=60)
        CGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGG                                                                                     <  SRR4302536.2151744/94‑1 (MQ=60)
          AGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCA                                                                                   <  SRR4302536.2940212/94‑1 (MQ=60)
          AGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCA                                                                                   >  SRR4302536.4908604/1‑94 (MQ=60)
          AGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCAAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCA                                                                                   <  SRR4302536.1830896/94‑1 (MQ=60)
            AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGT                                                                                 >  SRR4302536.3239405/1‑94 (MQ=60)
            AAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGT                                                                                 >  SRR4302536.2385958/1‑94 (MQ=60)
             AAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTA                                                                                >  SRR4302536.4178179/1‑94 (MQ=60)
             AAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTA                                                                                <  SRR4302536.2429826/94‑1 (MQ=60)
              AATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAA                                                                               <  SRR4302536.1204176/94‑1 (MQ=60)
               ATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAC                                                                              <  SRR4302536.2608559/94‑1 (MQ=60)
                 AGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAG                                                                            >  SRR4302536.412779/1‑94 (MQ=60)
                  GGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGC                                                                           <  SRR4302536.5573022/94‑1 (MQ=60)
                   GCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCT                                                                          <  SRR4302536.2151375/94‑1 (MQ=60)
                   GCGCGGCTGTTAGCATGAACATTATAACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGCAAACGTCATGCCGCTCATCCGGCCGTAACAGCT                                                                          >  SRR4302536.1748376/1‑94 (MQ=60)
                       GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      <  SRR4302536.2340362/94‑1 (MQ=60)
                       GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      >  SRR4302536.2319745/1‑94 (MQ=60)
                       GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      <  SRR4302536.2641089/94‑1 (MQ=60)
                       GGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTC                                                                      >  SRR4302536.6588983/1‑94 (MQ=60)
                        GCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCT                                                                     >  SRR4302536.690653/1‑94 (MQ=60)
                        GCTGTTAGCATAATCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGTGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCT                                                                     <  SRR4302536.5596009/94‑1 (MQ=60)
                         CTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTCACAGCTCTTCTT                                                                    >  SRR4302536.1737986/1‑94 (MQ=60)
                         CTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTT                                                                    >  SRR4302536.4726728/1‑94 (MQ=60)
                         CTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTT                                                                    <  SRR4302536.4169367/94‑1 (MQ=60)
                          TGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTC                                                                   >  SRR4302536.4755637/1‑94 (MQ=60)
                          TGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTC                                                                   >  SRR4302536.1020043/1‑94 (MQ=60)
                           GGTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCggaacacgccttcgtccg                                                                  >  SRR4302536.5020855/1‑76 (MQ=60)
                            TTAGCATGAACATTATCACTGGTGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302536.3369334/94‑1 (MQ=60)
                            TTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCG                                                                 <  SRR4302536.6277365/94‑1 (MQ=60)
                             TAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGA                                                                >  SRR4302536.3883262/1‑94 (MQ=60)
                              AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               >  SRR4302536.5971140/1‑94 (MQ=60)
                              AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               >  SRR4302536.1619374/1‑94 (MQ=60)
                              AGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGAT                                                               >  SRR4302536.935369/1‑94 (MQ=60)
                                CATGCACATTATCCCTGGCGAGCAGCGTGTCCGCTGTCAGCCAGCGGTAATCCTAATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             <  SRR4302536.4076849/94‑1 (MQ=60)
                                CATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAC                                                             >  SRR4302536.2005814/1‑94 (MQ=60)
                                 aggacCATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            <  SRR4302536.2649163/89‑1 (MQ=60)
                                 ATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACT                                                            >  SRR4302536.6479280/1‑94 (MQ=60)
                                  TGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTC                                                           >  SRR4302536.3069213/1‑94 (MQ=60)
                                  TGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTC                                                           <  SRR4302536.1540538/94‑1 (MQ=60)
                                   GAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCT                                                          <  SRR4302536.234823/94‑1 (MQ=60)
                                    AACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTG                                                         >  SRR4302536.5359067/1‑94 (MQ=60)
                                    AACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTG                                                         <  SRR4302536.1297487/94‑1 (MQ=60)
                                    AACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTG                                                         >  SRR4302536.4896646/1‑94 (MQ=60)
                                      CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       >  SRR4302536.2216316/1‑94 (MQ=60)
                                      CATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAA                                                       <  SRR4302536.2320026/94‑1 (MQ=60)
                                       ATTATCACTGGTCAGCAGCGCGTCCGACGTCAGCCAGCGGGAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAG                                                      >  SRR4302536.1027651/1‑94 (MQ=60)
                                        TTAGCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGC                                                     <  SRR4302536.3585315/94‑1 (MQ=60)
                                         TATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCG                                                    <  SRR4302536.3464184/94‑1 (MQ=60)
                                         TATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCG                                                    <  SRR4302536.421677/94‑1 (MQ=60)
                                           TCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAA                                                  <  SRR4302536.5819073/94‑1 (MQ=60)
                                             ACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAA                                                >  SRR4302536.6030602/1‑94 (MQ=60)
                                             ACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAA                                                >  SRR4302536.810018/1‑94 (MQ=60)
                                             ACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAA                                                <  SRR4302536.4607307/94‑1 (MQ=60)
                                             ACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGGAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGACAA                                                >  SRR4302536.5220122/1‑94 (MQ=60)
                                               TGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACC                                              >  SRR4302536.5993100/1‑94 (MQ=60)
                                                 GCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTACCGATACTCTGAAGCGAAAACCGA                                            <  SRR4302536.3215345/94‑1 (MQ=60)
                                                  CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           >  SRR4302536.2836842/1‑94 (MQ=60)
                                                  CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           <  SRR4302536.5858349/94‑1 (MQ=60)
                                                  CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           <  SRR4302536.415389/94‑1 (MQ=60)
                                                  CGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAG                                           <  SRR4302536.6791178/94‑1 (MQ=60)
                                                   GAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGC                                          >  SRR4302536.4499349/1‑94 (MQ=60)
                                                   GAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGC                                          >  SRR4302536.2018789/1‑94 (MQ=60)
                                                   GAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGC                                          >  SRR4302536.5825771/1‑94 (MQ=60)
                                                    AGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCA                                         >  SRR4302536.2175749/1‑94 (MQ=60)
                                                    AGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCA                                         >  SRR4302536.3131182/1‑94 (MQ=60)
                                                    AACAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCA                                         <  SRR4302536.125565/94‑1 (MQ=60)
                                                     GCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCAC                                        >  SRR4302536.1788495/1‑94 (MQ=60)
                                                      CAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACC                                       >  SRR4302536.6194937/1‑94 (MQ=60)
                                                      CAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACC                                       >  SRR4302536.2935533/1‑94 (MQ=60)
                                                       AGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCA                                      >  SRR4302536.855979/1‑94 (MQ=60)
                                                          GCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACAT                                   >  SRR4302536.1424622/1‑94 (MQ=60)
                                                          CCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACAT                                   <  SRR4302536.5121711/94‑1 (MQ=60)
                                                           CGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATA                                  <  SRR4302536.3140015/94‑1 (MQ=60)
                                                            GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 >  SRR4302536.2167133/1‑94 (MQ=60)
                                                            GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 <  SRR4302536.3910051/94‑1 (MQ=60)
                                                            GTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAG                                 <  SRR4302536.2852284/94‑1 (MQ=60)
                                                               CGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGA                              >  SRR4302536.1598205/1‑94 (MQ=60)
                                                                GACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGCGCACCACATAGTGAG                             >  SRR4302536.853755/1‑94 (MQ=60)
                                                                GACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAG                             <  SRR4302536.3530731/94‑1 (MQ=60)
                                                                CACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAG                             <  SRR4302536.3428673/94‑1 (MQ=60)
                                                                  CGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTG                           <  SRR4302536.4781662/94‑1 (MQ=60)
                                                                  CGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTG                           <  SRR4302536.100604/94‑1 (MQ=60)
                                                                   GTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGG                          >  SRR4302536.3686441/1‑94 (MQ=60)
                                                                      AGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGA                       >  SRR4302536.3072461/1‑94 (MQ=60)
                                                                       GCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAA                      <  SRR4302536.5016919/94‑1 (MQ=60)
                                                                         CAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAT                    <  SRR4302536.4901209/94‑1 (MQ=60)
                                                                           GCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCC                  <  SRR4302536.314731/94‑1 (MQ=60)
                                                                            CGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCG                 >  SRR4302536.5740981/1‑94 (MQ=60)
                                                                             GGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGT                <  SRR4302536.799960/94‑1 (MQ=60)
                                                                              GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               <  SRR4302536.5227176/94‑1 (MQ=60)
                                                                              GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               >  SRR4302536.6689545/1‑94 (MQ=60)
                                                                              GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               >  SRR4302536.4296445/1‑94 (MQ=60)
                                                                              GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               >  SRR4302536.6184745/1‑94 (MQ=60)
                                                                              GTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTG               <  SRR4302536.5191894/94‑1 (MQ=60)
                                                                               TAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCAGTGC              >  SRR4302536.673307/1‑94 (MQ=60)
                                                                                AATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCC             <  SRR4302536.3784581/94‑1 (MQ=60)
                                                                                 ATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCA            <  SRR4302536.4499828/94‑1 (MQ=60)
                                                                                    GTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCAAAACCCGAGCACCACACAGTGAGTGGTGAAATCCGTGCCAGAG         <  SRR4302536.6786472/94‑1 (MQ=60)
                                                                                     TCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGA        <  SRR4302536.238482/94‑1 (MQ=60)
                                                                                       ATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG      <  SRR4302536.2698016/94‑1 (MQ=60)
                                                                                        TGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT     >  SRR4302536.2786780/1‑94 (MQ=60)
                                                                                        TGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGT     <  SRR4302536.2684542/94‑1 (MQ=60)
                                                                                          CCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTA   <  SRR4302536.6445497/94‑1 (MQ=60)
                                                                                           CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  SRR4302536.6365174/1‑94 (MQ=60)
                                                                                           CGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  SRR4302536.906519/1‑94 (MQ=60)
                                                                                           |                                                                                             
CTTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAGTTAT  >  NC_000913/2123789‑2123973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑