Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1475	266.7	13469680	98.2%	13227225	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,163,496	T→C	S96P (TCC→CCC)	b2080 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,163,496	0	T	C	99.6%	812.8 / ‑4.6	248	S96P (TCC→CCC)	b2080	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (153/94); minor base A (0/1); total (153/95)
Fisher's exact test for biased strand distribution p-value = 3.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

Only 100 of 262 total aligned reads displayed.

TTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA  >  NC_000913/2163406‑2163583

ttCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCcccc                                                                                       <  2:6165423/93‑1 (MQ=255)

  cTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGt                                                                                     >  1:5251558/1‑93 (MQ=255)

  cTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGt                                                                                     <  1:3133261/93‑1 (MQ=255)

   tATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGtt                                                                                    <  2:5221707/93‑1 (MQ=255)

    aTTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTa                                                                                   >  2:1158772/1‑93 (MQ=255)

     tttCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTaa                                                                                  >  2:495108/1‑93 (MQ=255)

          aTCTGAAAGCCGCTAATCACCAAATTATCGGCTCCAGGCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTTCCCCCGGTTAAGCCa                                                                             >  1:6563827/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            >  1:6259915/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            >  2:247005/1‑93 (MQ=255)

           tCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCaa                                                                            <  1:5036311/93‑1 (MQ=255)

            cTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAAt                                                                           <  1:3039624/93‑1 (MQ=255)

            cTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAAt                                                                           <  2:3809229/93‑1 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          <  2:4500456/93‑1 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:4696916/1‑93 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:453029/1‑93 (MQ=255)

             tGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATg                                                                          >  1:3888358/1‑93 (MQ=255)

              gAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATgg                                                                         >  2:3940763/1‑93 (MQ=255)

              gAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATgg                                                                         >  2:273797/1‑93 (MQ=255)

                aaGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGca                                                                       >  2:6335605/1‑93 (MQ=255)

                 aGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcac                                                                      <  1:3244067/93‑1 (MQ=255)

                 aGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcac                                                                      >  1:4277600/1‑93 (MQ=255)

                  gCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcacc                                                                     >  2:1278494/1‑92 (MQ=255)

                  gCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGcaca                                                                     >  2:6414770/1‑93 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  >  1:5358786/1‑93 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  <  1:6404321/93‑1 (MQ=255)

                     gCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGc                                                                  >  2:4574832/1‑93 (MQ=255)

                        aaTCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAg                                                               >  1:1484974/1‑93 (MQ=255)

                        aaTCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAg                                                               >  1:5597452/1‑93 (MQ=255)

                          tcatcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAc                                                             <  2:1325945/93‑1 (MQ=255)

                          tcatcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAc                                                             >  1:3678299/1‑93 (MQ=255)

                           catcaAATTATCGGCTCCAGCCAGATGTACGACACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACg                                                            >  1:3588443/1‑93 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           >  1:5004068/1‑93 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           <  2:3058845/93‑1 (MQ=255)

                            atcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACgg                                                           <  1:2814242/93‑1 (MQ=255)

                             tcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGt                                                          >  2:1764609/1‑93 (MQ=255)

                             tcaAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGt                                                          <  2:6570764/93‑1 (MQ=255)

                               aaaTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGa                                                        <  1:4831450/93‑1 (MQ=255)

                               aaaTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGa                                                        >  2:4613304/1‑93 (MQ=255)

                                 aTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGaaa                                                      >  1:4020940/1‑93 (MQ=255)

                                 aTTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGaaa                                                      >  1:3716471/1‑93 (MQ=255)

                                  ttATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAg                                                     >  2:872423/1‑93 (MQ=255)

                                   tATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGa                                                    >  2:6730933/1‑93 (MQ=255)

                                    aTCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGAc                                                   <  2:3675787/93‑1 (MQ=255)

                                    aTCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGAc                                                   >  1:1788964/1‑93 (MQ=255)

                                     tCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACa                                                  <  1:4506570/93‑1 (MQ=255)

                                       ggCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAAt                                                <  1:2939122/93‑1 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               >  1:6698879/1‑93 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               <  1:5986816/93‑1 (MQ=255)

                                        gCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATa                                               <  2:5320409/93‑1 (MQ=255)

                                          tCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACg                                             >  1:1534258/1‑93 (MQ=255)

                                           ccagccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGt                                            >  1:3914679/1‑93 (MQ=255)

                                            cagccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTa                                           <  2:5514011/93‑1 (MQ=255)

                                              gccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAAt                                         <  1:4714424/93‑1 (MQ=255)

                                              gccagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAAt                                         <  2:4842833/93‑1 (MQ=255)

                                                cagATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTa                                       >  2:5251926/1‑93 (MQ=255)

                                                cagATGTACGCCACCGCGCAATCTCGTGAAACCAGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTa                                       <  1:2131175/93‑1 (MQ=255)

                                                 agATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTAc                                      <  1:1050637/93‑1 (MQ=255)

                                                  gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACg                                     >  1:6183775/1‑93 (MQ=255)

                                                  gATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACg                                     <  1:23532/93‑1 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    <  1:570546/93‑1 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    >  2:2405845/1‑93 (MQ=255)

                                                   aTGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGa                                    >  1:3807746/1‑93 (MQ=255)

                                                    tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAt                                   <  1:4635269/93‑1 (MQ=255)

                                                    tGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAt                                   >  2:2068964/1‑93 (MQ=255)

                                                      tACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGc                                 <  1:1377284/93‑1 (MQ=255)

                                                      tACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGc                                 <  2:5833924/93‑1 (MQ=255)

                                                       aCGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGcc                                <  2:21109/93‑1 (MQ=255)

                                                        cGCCACCGCGCAATCTCGTGAAACGGCAATTCCCCCCGTTAAAGCAAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCg                               <  1:851239/93‑1 (MQ=255)

                                                        cGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCg                               <  2:1509527/93‑1 (MQ=255)

                                                         gCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCgg                              >  1:271762/1‑93 (MQ=255)

                                                         gCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCgg                              <  1:5221336/93‑1 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  2:2641439/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  2:1571341/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           >  1:1696966/1‑93 (MQ=255)

                                                            aCCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCg                           <  1:4719125/93‑1 (MQ=255)

                                                              cgcgCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTg                         <  2:2981835/93‑1 (MQ=255)

                                                               gcgcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTgg                        <  2:351980/93‑1 (MQ=255)

                                                               gcgcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTgg                        <  2:4334399/93‑1 (MQ=255)

                                                                 gcAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgt                      >  2:2366184/1‑93 (MQ=255)

                                                                  cAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgtt                     >  1:1034668/1‑93 (MQ=255)

                                                                    aTCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgt                   >  2:1007828/1‑93 (MQ=255)

                                                                    aTCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgt                   <  2:5057307/93‑1 (MQ=255)

                                                                     tctcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgtt                  >  2:3596174/1‑93 (MQ=255)

                                                                     tctcGTAAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGgttgtt                  <  1:3416867/93‑1 (MQ=255)

                                                                      ctcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGttgttg                 <  1:4352236/93‑1 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                >  2:5139684/1‑93 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                >  2:4047497/1‑93 (MQ=255)

                                                                       tcGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                <  2:37256/93‑1 (MQ=255)

                                                                       tcGTGAAACCGGAATTCCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTgg                <  2:454244/93‑1 (MQ=255)

                                                                            aaaCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTcc           <  1:906892/93‑1 (MQ=255)

                                                                            aaaCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTcc           <  2:6509830/93‑1 (MQ=255)

                                                                             acccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACTTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCg          <  2:2220396/91‑1 (MQ=255)

                                                                              aCCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCgg         >  2:5737031/1‑93 (MQ=255)

                                                                               ccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgc        >  1:4226128/1‑93 (MQ=255)

                                                                               ccGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgc        <  2:6594623/93‑1 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      >  2:1782990/1‑93 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      <  2:4218020/93‑1 (MQ=255)

                                                                                 ggAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGgcgc      >  1:2010498/1‑93 (MQ=255)

                                                                                    aTTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCttt   >  2:6714594/1‑93 (MQ=255)

                                                                                    aTTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCttt   >  2:4894050/1‑93 (MQ=255)

TTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA  >  NC_000913/2163406‑2163583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA  >  NC_000913/2163403‑2163588

AAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCC                                                                                              >  SRR4302536.3113817/1‑94 (MQ=60)

AAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCC                                                                                              >  SRR4302536.2852873/1‑94 (MQ=60)

AAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCC                                                                                              >  SRR4302536.5021783/1‑94 (MQ=60)

 AATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCC                                                                                             >  SRR4302536.4262067/1‑94 (MQ=60)

  ATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCC                                                                                            <  SRR4302536.3713001/94‑1 (MQ=60)

   TTCTATTTCAATCTGAAAGCCGCTCATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCG                                                                                           >  SRR4302536.1429095/1‑94 (MQ=60)

    TCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGT                                                                                          >  SRR4302536.974676/1‑94 (MQ=60)

    TCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGT                                                                                          >  SRR4302536.5408039/1‑94 (MQ=60)

     CTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTT                                                                                         >  SRR4302536.2426607/1‑94 (MQ=60)

     CTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTT                                                                                         <  SRR4302536.3224906/94‑1 (MQ=60)

     CTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTT                                                                                         >  SRR4302536.5502891/1‑94 (MQ=60)

     CTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTT                                                                                         >  SRR4302536.6779695/1‑94 (MQ=60)

      TATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTA                                                                                        >  SRR4302536.3132192/1‑94 (MQ=60)

      TATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTA                                                                                        >  SRR4302536.1114087/1‑94 (MQ=60)

       ATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAA                                                                                       <  SRR4302536.4376382/94‑1 (MQ=60)

       ATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCACCGTTAA                                                                                       <  SRR4302536.169053/94‑1 (MQ=60)

       ATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGCGAAACCGGAATTGCCCCCGTTAA                                                                                       >  SRR4302536.2218743/1‑94 (MQ=60)

        TTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAA                                                                                      <  SRR4302536.6280149/94‑1 (MQ=60)

         TTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAG                                                                                     <  SRR4302536.3963472/94‑1 (MQ=60)

         TTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAG                                                                                     <  SRR4302536.39843/94‑1 (MQ=60)

          TCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGC                                                                                    <  SRR4302536.4114801/94‑1 (MQ=60)

           CAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTACAGCC                                                                                   >  SRR4302536.3232737/1‑94 (MQ=60)

            AATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCA                                                                                  >  SRR4302536.5856348/1‑94 (MQ=60)

            AATCTGAAAGCCGCTAATCACCAAATTATCGGCTCCAGGCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTTCCCCCGGTTAAGCCA                                                                                  >  SRR4302536.6759217/1‑94 (MQ=60)

             ATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAA                                                                                 >  SRR4302536.537112/1‑94 (MQ=60)

             ATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAA                                                                                 >  SRR4302536.6446417/1‑94 (MQ=60)

              TCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAAT                                                                                <  SRR4302536.5187267/94‑1 (MQ=60)

               CTGAAAGCCTCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               <  SRR4302536.4857892/94‑1 (MQ=60)

               CTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               >  SRR4302536.4003987/1‑94 (MQ=60)

               CTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               <  SRR4302536.3128996/94‑1 (MQ=60)

               CTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               >  SRR4302536.4838149/1‑94 (MQ=60)

               CTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               >  SRR4302536.3782050/1‑94 (MQ=60)

               CTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATG                                                                               >  SRR4302536.464924/1‑94 (MQ=60)

                TGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGG                                                                              >  SRR4302536.2914788/1‑94 (MQ=60)

                TGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGG                                                                              >  SRR4302536.6280520/1‑94 (MQ=60)

                TGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGG                                                                              >  SRR4302536.5902751/1‑94 (MQ=60)

                TGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGCACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGG                                                                              <  SRR4302536.4088500/94‑1 (MQ=60)

                 GAAAGCCGCTAATCAGCAAATTAGCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCGCCCGTTAAAGCCAATGGC                                                                             <  SRR4302536.1979551/94‑1 (MQ=60)

                  AAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGACACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATAGCA                                                                            >  SRR4302536.6517643/1‑94 (MQ=60)

                   AAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCAC                                                                           >  SRR4302536.4405130/1‑94 (MQ=60)

                    AGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACA                                                                          <  SRR4302536.3339141/94‑1 (MQ=60)

                      CCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGCAACCGGAACTGCCCCCGTTAAAGCCAATGGCACAAG                                                                        >  SRR4302536.483288/1‑94 (MQ=60)

                      CCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAG                                                                        <  SRR4302536.5181974/94‑1 (MQ=60)

                      CCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAG                                                                        >  SRR4302536.3888153/1‑94 (MQ=60)

                       CGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGC                                                                       >  SRR4302536.5518677/1‑94 (MQ=60)

                        GCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCC                                                                      <  SRR4302536.6595495/94‑1 (MQ=60)

                         CTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCA                                                                     >  SRR4302536.5280380/1‑94 (MQ=60)

                         CTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCA                                                                     <  SRR4302536.5415065/94‑1 (MQ=60)

                         CTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCA                                                                     <  SRR4302536.385531/94‑1 (MQ=60)

                         CTAATCATCAAATTATCGGCTCCACCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCA                                                                     >  SRR4302536.792514/1‑94 (MQ=60)

                          TAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAG                                                                    <  SRR4302536.2630237/94‑1 (MQ=60)

                          TAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAG                                                                    >  SRR4302536.3115015/1‑94 (MQ=60)

                          TAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAG                                                                    >  SRR4302536.1528844/1‑94 (MQ=60)

                          TAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAG                                                                    >  SRR4302536.5763853/1‑94 (MQ=60)

                            ATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAC                                                                  >  SRR4302536.4505550/1‑94 (MQ=60)

                            ATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGAC                                                                  >  SRR4302536.3788257/1‑94 (MQ=60)

                             TCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACG                                                                 <  SRR4302536.1065888/94‑1 (MQ=60)

                             TCATCAAATTATCGGCTCCAGCCAGATGTACGACACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACG                                                                 >  SRR4302536.3695789/1‑94 (MQ=60)

                              CATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGG                                                                <  SRR4302536.5095399/94‑1 (MQ=60)

                              CATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGG                                                                >  SRR4302536.5154208/1‑94 (MQ=60)

                              CATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAAACGGAATTGCCCCCGTTAAAGGCAATGGCACAAACCCGACGG                                                                >  SRR4302536.1788188/1‑94 (MQ=60)

                               ATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGT                                                               >  SRR4302536.4990357/1‑94 (MQ=60)

                               ATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGT                                                               <  SRR4302536.2897633/94‑1 (MQ=60)

                               ATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGT                                                               >  SRR4302536.5673734/1‑94 (MQ=60)

                                 CAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGA                                                             >  SRR4302536.3825355/1‑94 (MQ=60)

                                  aaagcATCGGATCCAGCCGGATGTACGCCACCGCGCAATCGCGGGAAACCGGAATTCCCCCCGTTAAAGCCAATGGCACAGGCCAGACGGTGAA                                                            <  SRR4302536.4412407/89‑1 (MQ=60)

                                  AAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAA                                                            <  SRR4302536.4976834/94‑1 (MQ=60)

                                  AAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAA                                                            >  SRR4302536.780997/1‑94 (MQ=60)

                                   AATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAA                                                           >  SRR4302536.4140241/1‑94 (MQ=60)

                                   AATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAA                                                           >  SRR4302536.3827576/1‑94 (MQ=60)

                                    ATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAAACGGAATTGCCCCCGTTAAAGCCAATCGCACAAGCCAGACGGAGAAAG                                                          >  SRR4302536.1730117/1‑94 (MQ=60)

                                      TATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGAC                                                        >  SRR4302536.1843009/1‑94 (MQ=60)

                                        TCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAA                                                      <  SRR4302536.4641779/94‑1 (MQ=60)

                                         CGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAAT                                                     <  SRR4302536.1544230/94‑1 (MQ=60)

                                          GGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATA                                                    >  SRR4302536.6413200/1‑94 (MQ=60)

                                          GGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATA                                                    >  SRR4302536.6897850/1‑94 (MQ=60)

                                          GGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATA                                                    >  SRR4302536.1997855/1‑94 (MQ=60)

                                          GGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATA                                                    <  SRR4302536.3025815/94‑1 (MQ=60)

                                           GCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATAC                                                   <  SRR4302536.6165310/94‑1 (MQ=60)

                                            CTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACG                                                  <  SRR4302536.6833031/94‑1 (MQ=60)

                                            CTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACG                                                  <  SRR4302536.280725/94‑1 (MQ=60)

                                            CTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACG                                                  <  SRR4302536.4652367/94‑1 (MQ=60)

                                            CTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACG                                                  >  SRR4302536.1579389/1‑94 (MQ=60)

                                             TCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGT                                                 >  SRR4302536.4031049/1‑94 (MQ=60)

                                               CAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAA                                               >  SRR4302536.5334073/1‑94 (MQ=60)

                                                AGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAAT                                              >  SRR4302536.1660972/1‑94 (MQ=60)

                                                 GCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATT                                             <  SRR4302536.4387140/94‑1 (MQ=60)

                                                 GCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATT                                             <  SRR4302536.4856246/94‑1 (MQ=60)

                                                  CTAGATGTACGCCACCAAGCAATCTCGTGAAACCGGAATTTCATCCGTTAAAGCCACTGGCACAAGCCAGAaggccaactacacaacgacacta                                            >  SRR4302536.1997322/1‑71 (MQ=54)

                                                  CCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTA                                            <  SRR4302536.441877/94‑1 (MQ=60)

                                                   CAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTAC                                           >  SRR4302536.3722146/1‑94 (MQ=60)

                                                   CAGATGTACGCCACCGCGCAATCTCGTGAAACCAGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTAC                                           <  SRR4302536.2195157/94‑1 (MQ=60)

                                                    AGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACG                                          >  SRR4302536.1293973/1‑94 (MQ=60)

                                                    AGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACG                                          >  SRR4302536.6367947/1‑94 (MQ=60)

                                                    AGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACG                                          <  SRR4302536.1081990/94‑1 (MQ=60)

                                                     GATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGA                                         <  SRR4302536.24188/94‑1 (MQ=60)

                                                     GATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGA                                         >  SRR4302536.3921250/1‑94 (MQ=60)

                                                      ATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGAT                                        <  SRR4302536.585053/94‑1 (MQ=60)

                                                       TGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCTCCTGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATG                                       <  SRR4302536.90391/94‑1 (MQ=60)

                                                       TGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATG                                       <  SRR4302536.2333672/94‑1 (MQ=60)

                                                       TGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATG                                       <  SRR4302536.2955184/94‑1 (MQ=60)

                                                       TGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATG                                       <  SRR4302536.4774571/94‑1 (MQ=60)

                                                       TGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATG                                       >  SRR4302536.4761661/1‑94 (MQ=60)

                                                        GTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGC                                      >  SRR4302536.4015146/1‑94 (MQ=60)

                                                         TACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCC                                     >  SRR4302536.4930673/1‑94 (MQ=60)

                                                         TACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCC                                     <  SRR4302536.1418310/94‑1 (MQ=60)

                                                           CGCCACCGCGCAATCTCGTGAAACGGCAATTCCCCCCGTTAAAGCAAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGG                                   <  SRR4302536.876275/94‑1 (MQ=60)

                                                           CGCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGG                                   >  SRR4302536.278997/1‑94 (MQ=60)

                                                            GCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGG                                  <  SRR4302536.5376913/94‑1 (MQ=60)

                                                            CCCACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGG                                  <  SRR4302536.1543835/94‑1 (MQ=60)

                                                              CACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCG                                >  SRR4302536.1746086/1‑94 (MQ=60)

                                                              CACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCG                                >  SRR4302536.3603903/1‑94 (MQ=60)

                                                               ACCGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGT                               <  SRR4302536.4861110/94‑1 (MQ=60)

                                                                 CGCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGG                             >  SRR4302536.3632305/1‑94 (MQ=60)

                                                                  GCGCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGG                            <  SRR4302536.4781441/94‑1 (MQ=60)

                                                                    GCAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTT                          >  SRR4302536.1065342/1‑94 (MQ=60)

                                                                     CAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTG                         >  SRR4302536.4480806/1‑94 (MQ=60)

                                                                     CAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTG                         >  SRR4302536.4785470/1‑94 (MQ=60)

                                                                     CAATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTG                         >  SRR4302536.1832947/1‑94 (MQ=60)

                                                                      AATCTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGT                        >  SRR4302536.6718534/1‑94 (MQ=60)

                                                                        TCTCGTAAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTG                      <  SRR4302536.3518560/94‑1 (MQ=60)

                                                                         CTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGG                     <  SRR4302536.4482446/94‑1 (MQ=60)

                                                                         CTCGTGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGG                     <  SRR4302536.4654125/94‑1 (MQ=60)

                                                                             TGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACATTACGTAATTACGATGTCGGGCGTGGGgtcatgacgtc                 >  SRR4302536.5225538/1‑83 (MQ=60)

                                                                             TGAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTC                 >  SRR4302536.6010173/1‑94 (MQ=60)

                                                                              GAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCC                >  SRR4302536.4197793/1‑94 (MQ=60)

                                                                              GAAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCC                >  SRR4302536.3084044/1‑94 (MQ=60)

                                                                               AAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCG               >  SRR4302536.6481008/1‑94 (MQ=60)

                                                                               AAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCG               >  SRR4302536.3257374/1‑94 (MQ=60)

                                                                               AAACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCG               <  SRR4302536.933610/94‑1 (MQ=60)

                                                                                 ACCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGC             >  SRR4302536.4351880/1‑94 (MQ=60)

                                                                                  CCGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCG            >  SRR4302536.2131905/1‑94 (MQ=60)

                                                                                   CGGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGC           >  SRR4302536.2070999/1‑94 (MQ=60)

                                                                                    GGAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCT          >  SRR4302536.3339161/1‑94 (MQ=60)

                                                                                     GAATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTT         >  SRR4302536.4912081/1‑94 (MQ=60)

                                                                                       ATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA       >  SRR4302536.5561375/1‑94 (MQ=60)

                                                                                       ATTGCCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTA       <  SRR4302536.483456/94‑1 (MQ=60)

                                                                                           CCCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAG   >  SRR4302536.1708741/1‑94 (MQ=60)

                                                                                            CCCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA  <  SRR4302536.3622168/94‑1 (MQ=60)

AAATTCTATTTCAATCTGAAAGCCGCTAATCATCAAATTATCGGCTCCAGCCAGATGTACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGA  >  NC_000913/2163403‑2163588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`