Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,185,017 Δ1 bp coding (38/339 nt) b2107 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,0140T.100.0% 926.9 / NA 199coding (35/339 nt)b2107hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (115/84);  total (115/84)

Only 100 of 224 total aligned reads displayed.

TATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGT  >  NC_000913/2184922‑2185102
                                                                                            |                                                                                        
tatTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCt                                                                                          >  2:2276538/1‑93 (MQ=255)
tatTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCt                                                                                          >  2:5912602/1‑93 (MQ=255)
tatTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCt                                                                                          >  2:3429112/1‑93 (MQ=255)
 atTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtt                                                                                         <  2:2661042/93‑1 (MQ=255)
 atTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtt                                                                                         <  1:1680087/93‑1 (MQ=255)
   tGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtttg                                                                                       >  1:2468421/1‑92 (MQ=255)
     tATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCtttgct                                                                                     >  1:3011354/1‑90 (MQ=255)
       tGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGGTTATGACCATTTAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTgg                                                                                  >  1:2904905/1‑93 (MQ=255)
       tGGCAGGAAAGATATGCGACTGATATAACAAATCCCCAAAGGGGGGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGGGCGC‑TTTGCTgg                                                                                  >  1:6648293/1‑93 (MQ=255)
        ggCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGt                                                                                 >  2:1703484/1‑93 (MQ=255)
         gCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGtt                                                                                >  2:1086210/1‑93 (MQ=255)
            ggAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTAcc                                                                             >  1:672900/1‑93 (MQ=255)
                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         >  2:5028668/1‑93 (MQ=255)
                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         >  2:4512083/1‑93 (MQ=255)
                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         >  2:5192826/1‑93 (MQ=255)
                aGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTg                                                                         <  1:1475598/93‑1 (MQ=255)
                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  1:4213068/93‑1 (MQ=255)
                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  2:1560970/93‑1 (MQ=255)
                  atatGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgcc                                                                       <  1:3753559/93‑1 (MQ=255)
                    atGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgc                                                                     >  1:2945516/1‑93 (MQ=255)
                    atGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgc                                                                     >  2:2439143/1‑93 (MQ=255)
                     tGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgcc                                                                    >  2:4892397/1‑93 (MQ=255)
                     tGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgcc                                                                    >  2:1441717/1‑93 (MQ=255)
                     tGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgcc                                                                    >  1:3224340/1‑93 (MQ=255)
                     tGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTgccgcc                                                                    >  1:2814717/1‑93 (MQ=255)
                        gACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTgg                                                                 >  1:1521642/1‑93 (MQ=255)
                         aCTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTggg                                                                >  1:5580609/1‑93 (MQ=255)
                         aCTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTggg                                                                >  1:3996125/1‑93 (MQ=255)
                           tGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGcc                                                              >  2:6022997/1‑93 (MQ=255)
                            gATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCg                                                             >  1:5918483/1‑93 (MQ=255)
                               atTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAc                                                          >  1:1165913/1‑93 (MQ=255)
                                ttACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAcc                                                         >  1:1924098/1‑93 (MQ=255)
                                ttACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAcc                                                         <  1:46840/93‑1 (MQ=255)
                                ttACAGATCCCCAAAGTGGAGAGCTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAcc                                                         >  1:538571/1‑93 (MQ=255)
                                     gATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCa                                                    >  1:4998373/1‑93 (MQ=255)
                                     gATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCa                                                    >  1:5043335/1‑93 (MQ=255)
                                      aTCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGGGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAc                                                   >  1:6624146/1‑93 (MQ=255)
                                          ccAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACcgcg                                               >  1:836170/1‑93 (MQ=255)
                                          ccAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACcgcg                                               >  1:1000139/1‑93 (MQ=255)
                                            aaaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCggg                                             >  1:1208354/1‑93 (MQ=255)
                                            aaaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCggg                                             <  2:5829478/93‑1 (MQ=255)
                                            aaaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCggg                                             <  2:1054660/93‑1 (MQ=255)
                                             aaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGt                                            <  2:2743283/93‑1 (MQ=255)
                                             aaGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGt                                            >  2:305109/1‑93 (MQ=255)
                                              aGTGGAGAGTTTATGACCATTANAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGtt                                           <  2:6704046/93‑1 (MQ=255)
                                               gTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTc                                          >  2:3535915/1‑93 (MQ=255)
                                                 ggAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGa                                        >  2:4794690/1‑93 (MQ=255)
                                                   agagTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGAcc                                      >  1:5513499/1‑93 (MQ=255)
                                                    gagTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCa                                     >  1:5633632/1‑93 (MQ=255)
                                                      gTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAAt                                   <  1:1863265/93‑1 (MQ=255)
                                                       tttATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATa                                  <  2:6095071/93‑1 (MQ=255)
                                                        ttATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATAc                                 <  1:78774/93‑1 (MQ=255)
                                                          aTGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATAcgt                               <  1:5310046/93‑3 (MQ=255)
                                                          aTGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCt                               <  1:2860307/93‑1 (MQ=255)
                                                          aTGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCt                               <  1:976927/93‑1 (MQ=255)
                                                           tGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTc                              <  2:6184795/93‑1 (MQ=255)
                                                           tGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTc                              <  2:3507706/93‑1 (MQ=255)
                                                           tGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTc                              >  1:2195417/1‑93 (MQ=255)
                                                             aCCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCgg                            <  1:3913224/93‑1 (MQ=255)
                                                             aCCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCgg                            <  2:2530720/93‑1 (MQ=255)
                                                              ccATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCggg                           <  1:800359/93‑1 (MQ=255)
                                                                aTTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGaa                         >  2:4390593/1‑93 (MQ=255)
                                                                aTTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGaa                         >  2:4647786/1‑93 (MQ=255)
                                                                 ttAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAt                        >  2:2999984/1‑93 (MQ=255)
                                                                 ttAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAt                        >  2:5045269/1‑93 (MQ=255)
                                                                  tAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGTCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAtt                       <  1:5433538/93‑1 (MQ=255)
                                                                  tAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAtt                       <  2:4177048/93‑1 (MQ=255)
                                                                  tAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAtt                       <  1:3186013/93‑1 (MQ=255)
                                                                   aaaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAttt                      <  2:5130789/93‑1 (MQ=255)
                                                                    aaaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTc                     <  1:5292512/93‑1 (MQ=255)
                                                                     aaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCt                    >  1:4231618/1‑93 (MQ=255)
                                                                     aaaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCt                    <  2:5313788/93‑1 (MQ=255)
                                                                      aaTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTa                   <  2:2551965/93‑1 (MQ=255)
                                                                       aTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTaa                  >  1:4413905/1‑93 (MQ=255)
                                                                       aTAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTaa                  >  2:1373942/1‑93 (MQ=255)
                                                                        tAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAg                 <  1:3356790/93‑1 (MQ=255)
                                                                        tAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAg                 <  2:4404291/93‑1 (MQ=255)
                                                                         aaGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGt                <  2:172276/93‑1 (MQ=255)
                                                                           gATGTTGCTGGGTGCGC‑TTTGCTGGTTCGCAGTGCCGCCTGGGCCGCACCAGCCCCCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAt              <  1:5607420/93‑1 (MQ=255)
                                                                           gATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAt              >  1:2088672/1‑93 (MQ=255)
                                                                           gATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAt              >  1:2705338/1‑93 (MQ=255)
                                                                            aTGTTGCTGGGTGCGC‑TTTGCTGGTTCCCAGTCCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATg             <  2:636569/93‑1 (MQ=255)
                                                                            aTGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATg             >  1:430898/1‑93 (MQ=255)
                                                                             tGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGa            <  2:5408632/93‑1 (MQ=255)
                                                                             tGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGa            >  1:4530644/1‑93 (MQ=255)
                                                                             tGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGa            <  1:2133363/93‑1 (MQ=255)
                                                                              gTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAg           >  1:5212675/1‑93 (MQ=255)
                                                                               ttGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGt          >  1:4389943/1‑93 (MQ=255)
                                                                                tGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGtt         >  1:4111445/1‑93 (MQ=255)
                                                                                tGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGtt         <  2:1908975/93‑1 (MQ=255)
                                                                                  cTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTaa       <  1:6382393/93‑1 (MQ=255)
                                                                                  cTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTaa       >  1:3435066/1‑93 (MQ=255)
                                                                                   tGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAag      >  1:5868446/1‑93 (MQ=255)
                                                                                    gggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagc     <  1:6270001/93‑2 (MQ=255)
                                                                                    gggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagc     >  2:2162816/1‑92 (MQ=255)
                                                                                    gggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagc     >  2:294362/1‑92 (MQ=255)
                                                                                    gggTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACATCGGGAATTTCTAAGTATGAGTTAaga     >  1:2329343/1‑92 (MQ=255)
                                                                                       tGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACTAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagcagt  <  1:5081029/93‑1 (MQ=255)
                                                                                       tGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATCCCTCGGGAATTTCTAAGTATGAGTTAagcagt  <  2:3978982/93‑1 (MQ=255)
                                                                                       tGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAagcagt  <  2:76893/93‑1 (MQ=255)
                                                                                            |                                                                                        
TATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGT  >  NC_000913/2184922‑2185102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGTTTCATTGC  >  NC_000913/2184924‑2185110
                                                                                             |                                                                                             
TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTG                                                                                               >  SRR4302536.2542941/1‑94 (MQ=60)
TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTG                                                                                               >  SRR4302536.4295131/1‑94 (MQ=60)
  GTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCT                                                                                            >  SRR4302536.3099868/1‑94 (MQ=60)
    ATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGGTTATGACCATTTAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGG                                                                                          >  SRR4302536.2990640/1‑94 (MQ=60)
    ATGGCAGGAAAGATATGCGACTGATATAACAAATCCCCAAAGGGGGGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGGGCGC‑TTTGCTGG                                                                                          >  SRR4302536.6845946/1‑94 (MQ=60)
     TGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGT                                                                                         >  SRR4302536.5788200/1‑94 (MQ=60)
         AGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACC                                                                                     >  SRR4302536.5411289/1‑94 (MQ=60)
         AGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACC                                                                                     >  SRR4302536.692597/1‑94 (MQ=60)
             AAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTG                                                                                 >  SRR4302536.5563408/1‑94 (MQ=60)
              AGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGC                                                                                >  SRR4302536.6535736/1‑94 (MQ=60)
              AGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGC                                                                                <  SRR4302536.1519225/94‑1 (MQ=60)
                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              <  SRR4302536.4338332/94‑1 (MQ=60)
                ATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCG                                                                              <  SRR4302536.3865707/94‑1 (MQ=60)
                 TATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGC                                                                             >  SRR4302536.3032347/1‑94 (MQ=60)
                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            >  SRR4302536.3318871/1‑94 (MQ=60)
                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            >  SRR4302536.4380584/1‑94 (MQ=60)
                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            <  SRR4302536.1990422/94‑1 (MQ=60)
                  ATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCC                                                                            >  SRR4302536.2898118/1‑94 (MQ=60)
                   TGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCT                                                                           <  SRR4302536.844053/94‑1 (MQ=60)
                     CGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGG                                                                         >  SRR4302536.1566422/1‑94 (MQ=60)
                     CGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGG                                                                         <  SRR4302536.4154197/94‑1 (MQ=60)
                      GACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGG                                                                        >  SRR4302536.5746681/1‑94 (MQ=60)
                      GACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGG                                                                        <  SRR4302536.3001644/94‑1 (MQ=60)
                      GACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGG                                                                        >  SRR4302536.4114708/1‑94 (MQ=60)
                         TGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCG                                                                     >  SRR4302536.6094774/1‑94 (MQ=60)
                          GATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGC                                                                    >  SRR4302536.823171/1‑94 (MQ=60)
                          GATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGC                                                                    >  SRR4302536.5877515/1‑94 (MQ=60)
                           ATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCA                                                                   >  SRR4302536.2847572/1‑94 (MQ=60)
                            TATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCAC                                                                  >  SRR4302536.1201000/1‑94 (MQ=60)
                             ATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACC                                                                 >  SRR4302536.1981991/1‑94 (MQ=60)
                             ATTACAGATCCCCAAAGTGGAGAGCTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACC                                                                 >  SRR4302536.552314/1‑94 (MQ=60)
                              TTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCA                                                                <  SRR4302536.48127/94‑1 (MQ=60)
                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            >  SRR4302536.2293332/1‑94 (MQ=60)
                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            >  SRR4302536.5148372/1‑94 (MQ=60)
                                  AGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCA                                                            >  SRR4302536.5194476/1‑94 (MQ=60)
                                   GATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAC                                                           >  SRR4302536.698473/1‑94 (MQ=60)
                                   GATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGGGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAC                                                           >  SRR4302536.6821165/1‑94 (MQ=60)
                                   GAACCCCAAAGCGGAGGGGTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCAC                                                           >  SRR4302536.5467698/1‑94 (MQ=60)
                                    ATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACC                                                          >  SRR4302536.1566778/1‑94 (MQ=60)
                                     TCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCG                                                         >  SRR4302536.572351/1‑94 (MQ=60)
                                      CCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGC                                                        <  SRR4302536.1554804/94‑1 (MQ=60)
                                      CCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGC                                                        >  SRR4302536.5650158/1‑94 (MQ=60)
                                       CCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCG                                                       >  SRR4302536.1029652/1‑94 (MQ=60)
                                       CCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCG                                                       >  SRR4302536.860764/1‑94 (MQ=60)
                                        CCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGG                                                      <  SRR4302536.1720311/94‑1 (MQ=60)
                                         CAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGG                                                     >  SRR4302536.1244665/1‑94 (MQ=60)
                                         CAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGG                                                     >  SRR4302536.4049464/1‑94 (MQ=60)
                                          AAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGT                                                    >  SRR4302536.2590702/1‑94 (MQ=60)
                                          AAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGT                                                    <  SRR4302536.393824/94‑1 (MQ=60)
                                           AATTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTT                                                   >  SRR4302536.4110881/1‑94 (MQ=60)
                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  >  SRR4302536.1767375/1‑94 (MQ=60)
                                            AGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCGGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTC                                                  <  SRR4302536.316419/94‑1 (MQ=60)
                                                GAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACC                                              >  SRR4302536.5677934/1‑94 (MQ=60)
                                                GAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACC                                              <  SRR4302536.1092744/94‑1 (MQ=60)
                                                 AGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCA                                             >  SRR4302536.5800988/1‑94 (MQ=60)
                                                 AGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCA                                             <  SRR4302536.3029068/94‑1 (MQ=60)
                                                 AGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCA                                             >  SRR4302536.2779094/1‑94 (MQ=60)
                                                    GTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATA                                          <  SRR4302536.1919377/94‑1 (MQ=60)
                                                      TTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACC                                        <  SRR4302536.6384718/94‑1 (MQ=60)
                                                      TTATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACC                                        <  SRR4302536.80895/94‑1 (MQ=60)
                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACGTC                                      <  SRR4302536.5468535/94‑1 (MQ=60)
                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      <  SRR4302536.1005761/94‑1 (MQ=60)
                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      <  SRR4302536.2944843/94‑1 (MQ=60)
                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      >  SRR4302536.6659458/1‑94 (MQ=60)
                                                        ATGACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTC                                      >  SRR4302536.2261681/1‑94 (MQ=60)
                                                          GACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGG                                    <  SRR4302536.5912547/94‑1 (MQ=60)
                                                           ACCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGG                                   <  SRR4302536.4029547/94‑1 (MQ=60)
                                                            CCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGA                                  <  SRR4302536.823825/94‑1 (MQ=60)
                                                            CCATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGA                                  >  SRR4302536.4470521/1‑94 (MQ=60)
                                                              ATTAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAAT                                <  SRR4302536.4443140/94‑1 (MQ=60)
                                                                TAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGTCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTT                              <  SRR4302536.5596002/94‑1 (MQ=60)
                                                                TAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTT                              <  SRR4302536.5425822/94‑1 (MQ=60)
                                                                TAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTT                              <  SRR4302536.3279425/94‑1 (MQ=60)
                                                                TAAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTT                              >  SRR4302536.2193237/1‑94 (MQ=60)
                                                                 AAAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTC                             >  SRR4302536.5664932/1‑94 (MQ=60)
                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            <  SRR4302536.5450334/94‑1 (MQ=60)
                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            <  SRR4302536.3494852/94‑1 (MQ=60)
                                                                  AAAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCT                            >  SRR4302536.4357538/1‑94 (MQ=60)
                                                                   AAATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTA                           >  SRR4302536.5201571/1‑94 (MQ=60)
                                                                    AATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAA                          >  SRR4302536.4546004/1‑94 (MQ=60)
                                                                     ATATGATGTTGCTTGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCTCACCCGCCTCCGCGGGTTCAACCAATACCTCGGGAATTTCTAAG                         <  SRR4302536.5548149/94‑1 (MQ=60)
                                                                     ATAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAG                         >  SRR4302536.2951034/1‑94 (MQ=60)
                                                                      TAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGT                        <  SRR4302536.837671/94‑1 (MQ=60)
                                                                      TAAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGT                        <  SRR4302536.3456420/94‑1 (MQ=60)
                                                                       AAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTA                       >  SRR4302536.3759832/1‑94 (MQ=60)
                                                                       AAGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTA                       >  SRR4302536.4532230/1‑94 (MQ=60)
                                                                        AGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAT                      >  SRR4302536.3039319/1‑94 (MQ=60)
                                                                        AGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAT                      >  SRR4302536.2786057/1‑94 (MQ=60)
                                                                        AGATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTAT                      >  SRR4302536.2151535/1‑94 (MQ=60)
                                                                         GATGTTGCTGGGTGCGC‑TTTGCTGGTTCGCAGTGCCGCCTGGGCCGCACCAGCCCCCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATG                     <  SRR4302536.5774022/94‑1 (MQ=60)
                                                                         GATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATG                     >  SRR4302536.442236/1‑94 (MQ=60)
                                                                         GATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATG                     <  SRR4302536.1336486/94‑1 (MQ=60)
                                                                         GATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATG                     <  SRR4302536.985683/94‑1 (MQ=60)
                                                                          ATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACTAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGA                    >  SRR4302536.6841097/1‑94 (MQ=60)
                                                                          ATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGA                    >  SRR4302536.5189343/1‑94 (MQ=60)
                                                                          ATGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGA                    >  SRR4302536.4666452/1‑94 (MQ=60)
                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   >  SRR4302536.5368021/1‑94 (MQ=60)
                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAG                   <  SRR4302536.2197389/94‑1 (MQ=60)
                                                                           TGTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGCCCGCACCAGCCACCGCGGGTTCGcacaatccctagtgaatttctgagtatcag                   >  SRR4302536.2233726/1‑64 (MQ=60)
                                                                            GTTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGT                  >  SRR4302536.4521060/1‑94 (MQ=60)
                                                                             TTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTT                 >  SRR4302536.4233407/1‑94 (MQ=60)
                                                                             TTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTT                 <  SRR4302536.2538336/94‑1 (MQ=60)
                                                                             GTGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCGGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTT                 <  SRR4302536.4592671/94‑1 (MQ=60)
                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                >  SRR4302536.4696302/1‑94 (MQ=60)
                                                                              TGCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTA                <  SRR4302536.2914023/94‑1 (MQ=60)
                                                                               GCTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAA               >  SRR4302536.3537339/1‑94 (MQ=60)
                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              >  SRR4302536.6043308/1‑94 (MQ=60)
                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              <  SRR4302536.4357276/94‑1 (MQ=60)
                                                                                CTGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAG              <  SRR4302536.6572756/94‑1 (MQ=60)
                                                                                 TGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGC             >  SRR4302536.6053821/1‑94 (MQ=60)
                                                                                 TGGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACATCGGGAATTTCTAAGTATGAGTTAAGA             >  SRR4302536.2400027/1‑94 (MQ=60)
                                                                                  GGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCA            <  SRR4302536.6456806/94‑1 (MQ=60)
                                                                                  GGGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCA            <  SRR4302536.3448350/94‑1 (MQ=60)
                                                                                   GGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAG           <  SRR4302536.5119419/94‑1 (MQ=60)
                                                                                   GGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAG           <  SRR4302536.5128623/94‑1 (MQ=60)
                                                                                   GGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAG           <  SRR4302536.3420094/94‑1 (MQ=60)
                                                                                   GGTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAG           >  SRR4302536.5502613/1‑94 (MQ=60)
                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          >  SRR4302536.6615648/1‑94 (MQ=60)
                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGT          <  SRR4302536.5722106/94‑1 (MQ=60)
                                                                                    GTGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGCATGAGTTAAGCAGT          <  SRR4302536.5267327/94‑1 (MQ=60)
                                                                                     TGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGGCGCACCAAACACCGCGGGTTCGACCAATACATCGGGCATTTCTAAGTATGAGTTAAGcagct         >  SRR4302536.3770747/1‑89 (MQ=60)
                                                                                     TGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACTAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTT         <  SRR4302536.5233081/94‑1 (MQ=60)
                                                                                     TGCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTT         >  SRR4302536.5167585/1‑94 (MQ=60)
                                                                                      GCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTT        <  SRR4302536.6567379/94‑1 (MQ=60)
                                                                                      GCGC‑TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTT        >  SRR4302536.1409204/1‑94 (MQ=60)
                                                                                       CGC‑TTTGCAGGTTACCAGTGCCGCCTGGGCCGCACCAGTCACCGCGGGTTAGACCAATACCTCGTGAATTTCTAAGTATGAGTTAAGCAGTTTC       >  SRR4302536.6821585/1‑94 (MQ=60)
                                                                                         gcTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCA      >  SRR4302536.2378813/3‑94 (MQ=60)
                                                                                          CTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCAT     >  SRR4302536.6816815/1‑94 (MQ=60)
                                                                                          CTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCAT     >  SRR4302536.1906105/1‑94 (MQ=60)
                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302536.6026681/94‑1 (MQ=60)
                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302536.4695909/94‑1 (MQ=60)
                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGG‑TCGGACAATACCCCCGGGATTTCTAAGGATGAAGTAAGCAGTTTCATTG   >  SRR4302536.4257323/1‑94 (MQ=46)
                                                                                           TTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGATTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302536.209804/94‑1 (MQ=60)
                                                                                           GTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCGCCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATT    <  SRR4302536.4336222/94‑1 (MQ=60)
                                                                                            TTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTG   <  SRR4302536.4425541/94‑1 (MQ=60)
                                                                                            TTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTG   >  SRR4302536.6506117/1‑94 (MQ=60)
                                                                                            TTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTG   <  SRR4302536.5682115/94‑1 (MQ=60)
                                                                                            TTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTG   <  SRR4302536.6335161/94‑1 (MQ=60)
                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTGC  >  SRR4302536.1362274/1‑94 (MQ=60)
                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGCAGTTTCATTGC  <  SRR4302536.5902892/94‑1 (MQ=60)
                                                                                             TGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGCGAATTTCTACGTATGAGTTAAGCAGTATCATTGC  >  SRR4302536.5590653/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATGACCATTAAAAATAAGATGTTGCTGGGTGCGCTTTTGCTGGTTACCAGTGCCGCCTGGGCCGCACCAGCCACCGCGGGTTCGACCAATACCTCGGGAATTTCTAAGTATGAGTTAAGTAGTTTCATTGC  >  NC_000913/2184924‑2185110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑