Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,281,741 T→C D76G (GAC→GGC)  yejK ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,281,7410TC99.6% 790.8 / ‑4.8 251D76G (GAC→GGC) yejKhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (129/121);  total (130/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01

Only 100 of 267 total aligned reads displayed.

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281651‑2281830
                                                                                          |                                                                                         
cAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                         <  1:1654097/93‑1 (MQ=255)
 aaaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCct                                                                                        >  1:1054548/1‑93 (MQ=255)
  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                       <  2:3676187/93‑1 (MQ=255)
  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                       <  1:4864069/93‑1 (MQ=255)
   aaCAGCACGAATCCGCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  1:5773041/93‑1 (MQ=255)
   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      >  2:109700/1‑93 (MQ=255)
     cAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTc                                                                                    <  2:2694661/93‑1 (MQ=255)
       gCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGc                                                                                  >  1:2135721/1‑93 (MQ=255)
         ccGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                <  2:33866/92‑1 (MQ=255)
          cGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCt                                                                               >  1:631347/1‑93 (MQ=255)
           caaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTg                                                                              <  2:2665860/92‑1 (MQ=255)
            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             <  1:4893089/93‑1 (MQ=255)
            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  1:173255/1‑93 (MQ=255)
            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAACCCTCTTCGCCCTGa                                                                             <  2:4318315/93‑1 (MQ=255)
              tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:1417093/1‑93 (MQ=255)
               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                          <  1:2511520/93‑1 (MQ=255)
               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                          <  1:2809632/93‑1 (MQ=255)
               ccACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGc                                                                          >  1:3545201/1‑93 (MQ=255)
                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                         >  1:5125799/1‑93 (MQ=255)
                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                         <  1:5337359/93‑1 (MQ=255)
                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                         <  2:4345700/93‑1 (MQ=255)
                  ccGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGt                                                                       <  1:2870533/93‑1 (MQ=255)
                    gTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTaa                                                                     <  2:411587/93‑1 (MQ=255)
                       aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcg                                                                  >  2:2385917/1‑93 (MQ=255)
                        gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 >  2:5242046/1‑93 (MQ=255)
                        gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 <  1:5052895/93‑1 (MQ=255)
                         cAAAAGGATTTTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                <  2:1326037/93‑1 (MQ=255)
                          aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCATGACGCTGTAAGCGCAg                                                               >  1:138130/1‑93 (MQ=255)
                           aaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGc                                                              >  1:712122/1‑93 (MQ=255)
                             aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  1:1138785/1‑93 (MQ=255)
                             aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  2:3216954/1‑93 (MQ=255)
                             aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  1:1338175/1‑93 (MQ=255)
                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:795263/1‑93 (MQ=255)
                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:6516060/1‑93 (MQ=255)
                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  2:5602870/93‑1 (MQ=255)
                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  1:5441632/93‑1 (MQ=255)
                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:1185150/1‑93 (MQ=255)
                              agATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  2:2009208/92‑1 (MQ=255)
                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  2:933117/93‑1 (MQ=255)
                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          >  2:6138631/1‑93 (MQ=255)
                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  1:3100278/93‑1 (MQ=255)
                                 tatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgc                                                        >  2:5443989/1‑93 (MQ=255)
                                  atTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcg                                                       <  2:6676414/93‑1 (MQ=255)
                                   tttCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcgc                                                      <  1:3664983/93‑1 (MQ=255)
                                       gcgaaTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGt                                                  <  1:6491265/90‑1 (MQ=255)
                                       gCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGt                                                  >  2:6307545/1‑93 (MQ=255)
                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                >  1:4315446/1‑93 (MQ=255)
                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                >  2:4629336/1‑93 (MQ=255)
                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                <  2:2244381/93‑1 (MQ=255)
                                           aTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAc                                              >  1:79762/1‑93 (MQ=255)
                                              cGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                           <  1:4249161/93‑1 (MQ=255)
                                               gTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctc                                          >  2:3439608/1‑93 (MQ=255)
                                                tCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctct                                         <  1:5959149/93‑1 (MQ=255)
                                                tCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctct                                         >  1:4124173/1‑93 (MQ=255)
                                                tCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctct                                         >  2:1380916/1‑93 (MQ=255)
                                                 cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        <  1:2061500/93‑1 (MQ=255)
                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAATCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       <  1:968489/93‑1 (MQ=255)
                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       <  2:719807/93‑1 (MQ=255)
                                                   cGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCg                                      >  1:2652468/1‑93 (MQ=255)
                                                     cAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCt                                    <  2:1996991/93‑1 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                  >  2:1831568/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                  >  2:5812947/1‑93 (MQ=255)
                                                       ggcggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTaa                                  <  2:3396801/93‑1 (MQ=255)
                                                         cggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAAc                                <  1:4728828/93‑1 (MQ=255)
                                                          ggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACa                               <  2:3620258/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              <  2:2160815/93‑1 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              >  2:1486196/1‑93 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              >  1:4510573/1‑93 (MQ=255)
                                                           gcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACaa                              >  2:3068561/1‑93 (MQ=255)
                                                            ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             >  1:3418626/1‑93 (MQ=255)
                                                            ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  1:3012154/93‑1 (MQ=255)
                                                            ccGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAg                             <  2:1671896/93‑1 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            >  2:4180064/1‑93 (MQ=255)
                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  1:4058091/93‑1 (MQ=255)
                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           <  2:4555316/93‑1 (MQ=255)
                                                                ttGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGt                         >  1:546402/1‑93 (MQ=255)
                                                                ttGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGt                         >  2:3532740/1‑93 (MQ=255)
                                                                ttGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGt                         >  2:5749103/1‑93 (MQ=255)
                                                                 tGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTa                        <  2:3988099/93‑1 (MQ=255)
                                                                  gCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTaa                       <  2:2347422/93‑1 (MQ=255)
                                                                     gcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCt                    <  1:147583/93‑1 (MQ=255)
                                                                     gcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCt                    >  1:5129391/1‑93 (MQ=255)
                                                                     gcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCt                    >  1:5149247/1‑93 (MQ=255)
                                                                     gcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCt                    <  1:1020929/93‑1 (MQ=255)
                                                                      ggcgGCTAAATGCCAGGAAGCCCTCTCCGCCCGGACGCTGTAAGCGCAGCGTCGGCGCCGTTTCACTCTCTTCGCAAAACAAGCCGTAAGCtt                   <  2:5963403/92‑1 (MQ=255)
                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                  >  2:636908/1‑93 (MQ=255)
                                                                        cgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                 <  2:1106230/93‑1 (MQ=255)
                                                                        cgGCTAAATGCCAGGAACCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTa                 <  1:6322538/93‑1 (MQ=255)
                                                                         ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                <  2:2537953/93‑1 (MQ=255)
                                                                          gCTAAATGCCAGGAGCCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt               <  2:5196400/93‑1 (MQ=255)
                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt               <  1:2329418/93‑1 (MQ=255)
                                                                           cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              <  1:1884516/93‑1 (MQ=255)
                                                                           cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              >  2:1635253/1‑93 (MQ=255)
                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             <  2:4894430/93‑1 (MQ=255)
                                                                             aaaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttttt            <  2:5696953/93‑1 (MQ=255)
                                                                              aaTGCCAGAAAGCCTTCTTCGCCCTGACGCTGTAAGCGCAGCGCCTGCGCAAGTTCACTCTCTTCGCTAAAAAAGCCGTAAGCTTTATTTTTg           <  1:277079/93‑1 (MQ=255)
                                                                                tGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgc         >  1:558601/1‑93 (MQ=255)
                                                                                 gCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcg        >  1:3360000/1‑93 (MQ=255)
                                                                                  caaGGAAGCCCTCTTCGCCCTGCCGCTGTAGGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcgc       <  2:3007657/91‑1 (MQ=255)
                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCCCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   <  2:3785262/93‑1 (MQ=255)
                                                                                          |                                                                                         
CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281651‑2281830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACC  >  NC_000913/2281648‑2281833
                                                                                             |                                                                                            
TGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGAAATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGC                                                                                              >  SRR4302536.1793129/1‑94 (MQ=60)
 GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCC                                                                                             <  SRR4302536.6901439/94‑1 (MQ=60)
 GGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCC                                                                                             >  SRR4302536.115752/1‑94 (MQ=60)
  GCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCC                                                                                            <  SRR4302536.6295572/94‑1 (MQ=60)
   CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                           >  SRR4302536.1086064/1‑94 (MQ=60)
   CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                           >  SRR4302536.3665378/1‑94 (MQ=60)
   CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                           <  SRR4302536.1756006/94‑1 (MQ=60)
   CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCT                                                                                           <  SRR4302536.1702154/94‑1 (MQ=60)
    AAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTC                                                                                          >  SRR4302536.79349/1‑94 (MQ=60)
     AAACAGCACGAATCCATCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                         <  SRR4302536.289205/94‑1 (MQ=60)
     AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                         <  SRR4302536.5593556/94‑1 (MQ=60)
     AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                         <  SRR4302536.5010444/94‑1 (MQ=60)
     AAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCT                                                                                         <  SRR4302536.144174/94‑1 (MQ=60)
      AACAGCACGAATCCGCCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                        <  SRR4302536.5944826/94‑1 (MQ=60)
      AACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTT                                                                                        <  SRR4302536.5223027/94‑1 (MQ=60)
       ACAGCACGAATCCACCGTCAGCAAAAGGATTTTTGGCTAATTGGTCACGCAGGCGCCCGGTTGCCGCGCGGCTAAAGGCCAGGAAGCCCTCTTC                                                                                       >  SRR4302536.4930546/1‑94 (MQ=60)
       ACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTC                                                                                       >  SRR4302536.6115992/1‑94 (MQ=60)
         AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                     >  SRR4302536.2199816/1‑94 (MQ=60)
         AGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGC                                                                                     >  SRR4302536.94468/1‑94 (MQ=60)
           CACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCC                                                                                   >  SRR4302536.3959234/1‑94 (MQ=60)
            ACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCT                                                                                  >  SRR4302536.650039/1‑94 (MQ=60)
            ACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCT                                                                                  <  SRR4302536.4567219/94‑1 (MQ=60)
             CGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCGCTTCGCCCTG                                                                                 <  SRR4302536.2146610/94‑1 (MQ=60)
              GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCGCTCTTCGCCCTGA                                                                                <  SRR4302536.1720894/94‑1 (MQ=60)
              GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGA                                                                                >  SRR4302536.177783/1‑94 (MQ=60)
              GAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGA                                                                                >  SRR4302536.5626879/1‑94 (MQ=60)
               AATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAC                                                                               <  SRR4302536.5040266/94‑1 (MQ=60)
                ATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACG                                                                              >  SRR4302536.1459225/1‑94 (MQ=60)
                 TCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGC                                                                             >  SRR4302536.3651195/1‑94 (MQ=60)
                  CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                            <  SRR4302536.2892926/94‑1 (MQ=60)
                  CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                            >  SRR4302536.5278939/1‑94 (MQ=60)
                  CCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCT                                                                            <  SRR4302536.2587253/94‑1 (MQ=60)
                   CACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTG                                                                           <  SRR4302536.5496817/94‑1 (MQ=60)
                   CACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGGTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTacggctg                                                                           >  SRR4302536.4452723/1‑87 (MQ=60)
                     CCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTA                                                                         <  SRR4302536.2955295/94‑1 (MQ=60)
                           GCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCA                                                                   <  SRR4302536.5204281/94‑1 (MQ=60)
                           GCAAAAGGATATGTCGCTAATCCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCA                                                                   <  SRR4302536.6610662/94‑1 (MQ=60)
                            CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAG                                                                  <  SRR4302536.2679954/94‑1 (MQ=60)
                            CAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCATGACGCTGTAAGCGCAG                                                                  >  SRR4302536.141739/1‑94 (MQ=60)
                             AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGC                                                                 <  SRR4302536.540511/94‑1 (MQ=60)
                             AAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGC                                                                 >  SRR4302536.732930/1‑94 (MQ=60)
                              AAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCG                                                                >  SRR4302536.1481689/1‑94 (MQ=60)
                               AAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGT                                                               >  SRR4302536.1173022/1‑94 (MQ=60)
                               AAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGT                                                               >  SRR4302536.1378090/1‑94 (MQ=60)
                                AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                              >  SRR4302536.5681476/1‑94 (MQ=60)
                                AGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTC                                                              >  SRR4302536.3820871/1‑94 (MQ=60)
                                 GGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCT                                                             <  SRR4302536.5604386/94‑1 (MQ=60)
                                 AGATATTTCACTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCT                                                             <  SRR4302536.3289111/94‑1 (MQ=60)
                                  GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                            >  SRR4302536.441150/1‑94 (MQ=60)
                                  GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                            <  SRR4302536.3190798/94‑1 (MQ=60)
                                  GATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTG                                                            <  SRR4302536.5287069/94‑1 (MQ=60)
                                   ATATTTCGCTAATTCGTCACGCAGGTGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                           >  SRR4302536.3736384/1‑94 (MQ=60)
                                   ATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                           >  SRR4302536.1279209/1‑94 (MQ=60)
                                   ATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGC                                                           >  SRR4302536.4205107/1‑94 (MQ=60)
                                      TTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCC                                                        <  SRR4302536.1172694/94‑1 (MQ=60)
                                      TTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCC                                                        <  SRR4302536.3774554/94‑1 (MQ=60)
                                       TTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCA                                                       >  SRR4302536.4289656/1‑94 (MQ=60)
                                       TTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCA                                                       <  SRR4302536.1792156/94‑1 (MQ=60)
                                        TCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAG                                                      <  SRR4302536.1862392/94‑1 (MQ=60)
                                        TCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCCTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAG                                                      <  SRR4302536.2012367/94‑1 (MQ=60)
                                         CGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGT                                                     >  SRR4302536.1489945/1‑94 (MQ=60)
                                          GCGAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTT                                                    <  SRR4302536.6684816/94‑1 (MQ=60)
                                           CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                   <  SRR4302536.6368553/94‑1 (MQ=60)
                                           CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                   <  SRR4302536.5647333/94‑1 (MQ=60)
                                           CTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTC                                                   >  SRR4302536.4444641/1‑94 (MQ=60)
                                            TAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGGCCTGCCGCTGTAAGTGCAGCGTCTGCGCCAGTTCA                                                  >  SRR4302536.3504056/1‑94 (MQ=60)
                                            TAATTCGTCACGCAGGAGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCA                                                  >  SRR4302536.1111703/1‑94 (MQ=60)
                                             AATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAC                                                 >  SRR4302536.81902/1‑94 (MQ=60)
                                             AATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAC                                                 >  SRR4302536.4767048/1‑94 (MQ=60)
                                              ATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACT                                                >  SRR4302536.2393087/1‑94 (MQ=60)
                                                TCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCT                                              >  SRR4302536.863499/1‑94 (MQ=60)
                                                TCGTCACGCAGGCGGCCGGTTACCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCT                                              <  SRR4302536.2145888/94‑1 (MQ=60)
                                                 CGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCCGTTCACTCTC                                             >  SRR4302536.5280204/1‑94 (MQ=60)
                                                 CGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTC                                             <  SRR4302536.4375729/94‑1 (MQ=60)
                                                 CGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTC                                             >  SRR4302536.1058341/1‑94 (MQ=60)
                                                  GTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCT                                            >  SRR4302536.4246442/1‑94 (MQ=60)
                                                   TCACGCAGGTGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGGTCGCTCTCTT                                           >  SRR4302536.6916102/1‑94 (MQ=60)
                                                   TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTT                                           <  SRR4302536.4709174/94‑1 (MQ=60)
                                                   TCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTT                                           <  SRR4302536.6136725/94‑1 (MQ=60)
                                                    CACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTC                                          <  SRR4302536.5138690/94‑1 (MQ=60)
                                                    CACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTC                                          <  SRR4302536.2123582/94‑1 (MQ=60)
                                                     ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAATCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                         <  SRR4302536.997080/94‑1 (MQ=60)
                                                     ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCTCTCTCTTCG                                         <  SRR4302536.5606836/94‑1 (MQ=60)
                                                     ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                         >  SRR4302536.2731898/1‑94 (MQ=60)
                                                     ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                         >  SRR4302536.2945706/1‑94 (MQ=60)
                                                     ACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCG                                         <  SRR4302536.4959443/94‑1 (MQ=60)
                                                       GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                       <  SRR4302536.1388159/94‑1 (MQ=60)
                                                       GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                       >  SRR4302536.2658859/1‑94 (MQ=60)
                                                       GCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCT                                       >  SRR4302536.3497774/1‑94 (MQ=60)
                                                        CAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTA                                      >  SRR4302536.283248/1‑94 (MQ=60)
                                                         aagaGGCCGTTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGAAGCAGTAAGCGCAGAGTCTGCGCCAGTTCACTCTCTTCGCTTA                                     >  SRR4302536.4019366/5‑94 (MQ=60)
                                                            CGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACA                                  <  SRR4302536.4871112/94‑1 (MQ=60)
                                                            CGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACA                                  <  SRR4302536.3314094/94‑1 (MQ=60)
                                                            CGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACA                                  <  SRR4302536.3522981/94‑1 (MQ=60)
                                                             GGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAA                                 >  SRR4302536.4645876/1‑94 (MQ=60)
                                                              GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                >  SRR4302536.3520370/1‑94 (MQ=60)
                                                              GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                <  SRR4302536.3496857/94‑1 (MQ=60)
                                                              GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                >  SRR4302536.4428467/1‑94 (MQ=60)
                                                              GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAG                                <  SRR4302536.6108356/94‑1 (MQ=60)
                                                               CCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGC                               <  SRR4302536.3100685/94‑1 (MQ=60)
                                                                CGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCC                              >  SRR4302536.723044/1‑94 (MQ=60)
                                                                CGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCC                              <  SRR4302536.2750943/94‑1 (MQ=60)
                                                                CGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCC                              <  SRR4302536.4178383/94‑1 (MQ=60)
                                                                CGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCC                              >  SRR4302536.2292459/1‑94 (MQ=60)
                                                                 GGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCG                             >  SRR4302536.5796202/1‑94 (MQ=60)
                                                                 CGTTGCCGCGCGGCTAAATGCCAGGAAGACCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGATCACTCTCTTCGCTAAACAAGCCG                             >  SRR4302536.3279232/1‑94 (MQ=60)
                                                                  GTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGT                            >  SRR4302536.560347/1‑94 (MQ=60)
                                                                   TTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACCCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTA                           <  SRR4302536.4279857/94‑1 (MQ=60)
                                                                    TGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAA                          <  SRR4302536.1763326/94‑1 (MQ=60)
                                                                    TGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAA                          >  SRR4302536.4935465/1‑94 (MQ=60)
                                                                     GCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAG                         <  SRR4302536.2553811/94‑1 (MQ=60)
                                                                     GCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCGGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAG                         <  SRR4302536.5294306/94‑1 (MQ=60)
                                                                       CGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCT                       >  SRR4302536.5302898/1‑94 (MQ=60)
                                                                       CGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCT                       >  SRR4302536.5282602/1‑94 (MQ=60)
                                                                        GCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTT                      <  SRR4302536.151427/94‑1 (MQ=60)
                                                                        GCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTT                      <  SRR4302536.1051153/94‑1 (MQ=60)
                                                                        CCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTT                      <  SRR4302536.5375302/94‑1 (MQ=60)
                                                                           CGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                   >  SRR4302536.5050728/1‑94 (MQ=60)
                                                                           CGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                   <  SRR4302536.5256565/94‑1 (MQ=60)
                                                                           CGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                   <  SRR4302536.6510856/94‑1 (MQ=60)
                                                                           CGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                   >  SRR4302536.3016042/1‑94 (MQ=60)
                                                                           CGGCTAAATGCCAGGAACCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAT                   <  SRR4302536.6510857/94‑1 (MQ=60)
                                                                            GGCTAAATGCCAGGAGGCCCTCTTCGCCCTGACGCTGTACGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATT                  <  SRR4302536.2961361/94‑1 (MQ=60)
                                                                            GGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATT                  <  SRR4302536.6538059/94‑1 (MQ=60)
                                                                             GCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTT                 <  SRR4302536.2400103/94‑1 (MQ=60)
                                                                             GCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTT                 <  SRR4302536.2863678/94‑1 (MQ=60)
                                                                             GCTAAATGCCAGAAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTT                 <  SRR4302536.1520286/94‑1 (MQ=60)
                                                                              CTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTT                <  SRR4302536.1941208/94‑1 (MQ=60)
                                                                               TAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAACCGCAGCGTCGGCGCCATTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTT               <  SRR4302536.4292959/94‑1 (MQ=60)
                                                                                AAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTG              <  SRR4302536.6463271/94‑1 (MQ=60)
                                                                                 AATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGG             <  SRR4302536.134100/94‑1 (MQ=60)
                                                                                 AATGCCAGAAAGCCTTCTTCGCCCTGACGCTGTAAGCGCAGCGCCTGCGCAAGTTCACTCTCTTCGCTAAAAAAGCCGTAAGCTTTATTTTTGG             <  SRR4302536.284445/94‑1 (MQ=60)
                                                                                 AATGCCAGAAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGG             <  SRR4302536.1453504/94‑1 (MQ=60)
                                                                                  ATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGC            >  SRR4302536.572830/1‑94 (MQ=60)
                                                                                   TGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCG           >  SRR4302536.3459734/1‑94 (MQ=60)
                                                                                        GGAACCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATA      <  SRR4302536.2397477/94‑1 (MQ=60)
                                                                                         GAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGAGCTATAG     >  SRR4302536.3115445/1‑94 (MQ=60)
                                                                                          AAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGA    <  SRR4302536.5026002/94‑1 (MQ=60)
                                                                                          AAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGA    >  SRR4302536.689124/1‑94 (MQ=60)
                                                                                           AGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGTCGCTATAGAC   >  SRR4302536.640731/1‑94 (MQ=60)
                                                                                            GCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACC  <  SRR4302536.758633/94‑1 (MQ=60)
                                                                                            GCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACC  >  SRR4302536.490056/1‑94 (MQ=60)
                                                                                             |                                                                                            
TGGCAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAGACC  >  NC_000913/2281648‑2281833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑