Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,330,191 A→G intergenic (‑2374/+2165) b2226 ← / ← b2229 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,330,1910AG99.6% 865.3 / ‑4.5 264intergenic (‑2374/+2165)b2226/b2229hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (139/124);  minor base T (0/1);  total (139/125)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 266 total aligned reads displayed.

ACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAA  >  NC_000913/2330101‑2330280
                                                                                          |                                                                                         
  cAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGt                                                                                       <  1:6064639/93‑1 (MQ=255)
   aGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGtt                                                                                      >  2:3021221/1‑93 (MQ=255)
     gCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTAc                                                                                    <  1:1008415/93‑1 (MQ=255)
       tGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACgg                                                                                  >  1:2174728/1‑93 (MQ=255)
        ggTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACggg                                                                                 <  1:1891912/93‑1 (MQ=255)
        ggTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACggg                                                                                 <  2:4962435/93‑1 (MQ=255)
            aTGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGtata                                                                             <  1:4344390/93‑1 (MQ=255)
            aTGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGtata                                                                             <  1:2230352/93‑1 (MQ=255)
             tGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGGTACGGGTATAc                                                                            >  2:571252/1‑93 (MQ=255)
              gggTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACa                                                                           <  1:4265481/93‑1 (MQ=255)
              gggTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGCTCTGAAAACTGTACTGGCCGTTACGGGTATAcg                                                                           >  2:2664038/1‑92 (MQ=255)
               ggTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAg                                                                          >  2:5418562/1‑93 (MQ=255)
               ggTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAg                                                                          >  2:2264503/1‑93 (MQ=255)
                 ttttGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGcc                                                                        >  2:4726025/1‑92 (MQ=255)
                 ttttGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCa                                                                        >  2:2463916/1‑93 (MQ=255)
                 ttttGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCa                                                                        >  1:903824/1‑93 (MQ=255)
                  tttGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCAc                                                                       >  2:3174520/1‑93 (MQ=255)
                  tttGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCAc                                                                       >  2:5038436/1‑93 (MQ=255)
                     gTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGa                                                                    >  1:5245850/1‑93 (MQ=255)
                      tCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGaa                                                                   >  2:3664247/1‑93 (MQ=255)
                       ccGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGaaa                                                                  <  1:1680744/93‑1 (MQ=255)
                        cGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGaaaa                                                                 <  2:4872149/93‑1 (MQ=255)
                         gTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGaaaaa                                                                <  1:735326/93‑1 (MQ=255)
                          ttttCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAg                                                               >  2:777969/1‑93 (MQ=255)
                           tttCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGt                                                              <  1:5044856/93‑1 (MQ=255)
                             taaCCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGa                                                            <  1:4698197/91‑1 (MQ=255)
                               aCCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATg                                                          >  2:42534/1‑93 (MQ=255)
                               aCCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATg                                                          >  1:177527/1‑93 (MQ=255)
                                cccGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGt                                                         >  1:5976754/1‑93 (MQ=255)
                                cccGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGt                                                         <  2:2549924/93‑1 (MQ=255)
                                cccGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGt                                                         <  1:3617901/93‑1 (MQ=255)
                                 ccGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGtt                                                        >  1:4809875/1‑93 (MQ=255)
                                  cGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTa                                                       <  1:2041345/93‑1 (MQ=255)
                                   ggATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAg                                                      <  1:4513983/93‑1 (MQ=255)
                                   ggATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAg                                                      <  2:6210243/93‑1 (MQ=255)
                                    gATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAgg                                                     <  2:6037532/93‑1 (MQ=255)
                                     atatCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgc                                                    <  1:534650/93‑1 (MQ=255)
                                     atatCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgc                                                    >  1:6159474/1‑93 (MQ=255)
                                      tatCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcg                                                   >  1:5524588/1‑93 (MQ=255)
                                       atCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                                                  <  2:4287081/93‑1 (MQ=255)
                                       atCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                                                  >  2:1016559/1‑93 (MQ=255)
                                       atCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGgcgc                                                  >  1:1854343/1‑93 (MQ=255)
                                        tCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCa                                                 >  2:1826708/1‑93 (MQ=255)
                                         ccAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCaa                                                >  1:6393699/1‑93 (MQ=255)
                                          cAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCaaa                                               <  1:3385560/93‑1 (MQ=255)
                                           aGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAGCTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAg                                              <  2:2830895/93‑1 (MQ=255)
                                            gCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAgg                                             >  2:2172328/1‑93 (MQ=255)
                                               ggggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAt                                          >  2:4166537/1‑93 (MQ=255)
                                               ggggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAt                                          <  1:3693751/93‑1 (MQ=255)
                                               ggggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAt                                          <  2:2711501/93‑1 (MQ=255)
                                                gggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAtt                                         <  1:3761837/93‑1 (MQ=255)
                                                gggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAtt                                         >  2:5209670/1‑93 (MQ=255)
                                                gggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAtt                                         <  2:1153692/93‑1 (MQ=255)
                                                gggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCCCAAAGGAAtt                                         <  1:585793/93‑1 (MQ=255)
                                                 ggAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTg                                        >  2:2515690/1‑93 (MQ=255)
                                                   atcGGCAGCTTTGATCCCGGCGTGCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCt                                      <  1:1073209/91‑1 (MQ=255)
                                                    aCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTc                                     <  2:1279708/93‑1 (MQ=255)
                                                    aCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTc                                     <  2:1235730/93‑1 (MQ=255)
                                                    aCGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTc                                     >  1:5657720/1‑93 (MQ=255)
                                                     cGGCATCTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCa                                    <  1:3517745/93‑1 (MQ=255)
                                                     cGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCa                                    >  1:2544734/1‑93 (MQ=255)
                                                      ggCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAc                                   <  2:4521268/93‑1 (MQ=255)
                                                      ggCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAc                                   <  2:6403107/93‑1 (MQ=255)
                                                       gCATCTTGGATCCCGGCGTTCTGAAAACTGTCCTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGGGCAAAGGAATTGCTCACt                                  <  2:617761/93‑1 (MQ=255)
                                                            tttGATCCCGGCTTGCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGTCGCAAAGGAATTGCTCACTGGAAc                             <  1:6229951/93‑1 (MQ=255)
                                                            tttGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAc                             <  2:5854343/93‑1 (MQ=255)
                                                            tttGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAc                             >  2:4948690/1‑93 (MQ=255)
                                                             ttGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAcc                            <  2:6731277/93‑1 (MQ=255)
                                                              tGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAccc                           >  2:5932716/1‑93 (MQ=255)
                                                               gATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCg                          <  1:773972/93‑1 (MQ=255)
                                                               gATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCg                          <  1:2992838/93‑1 (MQ=255)
                                                                aTCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCgg                         >  1:3934695/1‑93 (MQ=255)
                                                                 tCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCggg                        >  1:1558157/1‑93 (MQ=255)
                                                                 tCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCggg                        >  1:1222571/1‑93 (MQ=255)
                                                                 tCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCggg                        >  2:203289/1‑93 (MQ=255)
                                                                   ccGGCGTTCTGAAAACTGTACTGGCCGTTTCGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCt                      <  1:6596576/93‑1 (MQ=255)
                                                                   ccGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCt                      >  1:1975923/1‑93 (MQ=255)
                                                                    cGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCtt                     <  1:1522022/93‑1 (MQ=255)
                                                                      gCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCa                   <  2:5657490/93‑1 (MQ=255)
                                                                        gTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATa                 <  1:5734994/93‑1 (MQ=255)
                                                                         ttCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAc                >  1:5584490/1‑93 (MQ=255)
                                                                           cTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTg              >  2:52362/1‑93 (MQ=255)
                                                                            tGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTgg             <  2:4602671/93‑1 (MQ=255)
                                                                            tGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTgg             >  2:380169/1‑93 (MQ=255)
                                                                            tGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTgg             >  2:1993213/1‑93 (MQ=255)
                                                                            tGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTgg             >  1:1329824/1‑93 (MQ=255)
                                                                            tGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGGGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTgg             >  1:5073486/1‑93 (MQ=255)
                                                                             gAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTggg            >  1:2932853/1‑93 (MQ=255)
                                                                             gAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTggg            >  1:4106015/1‑93 (MQ=255)
                                                                              aaaaCTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGt           <  1:240934/93‑1 (MQ=255)
                                                                               aaaCTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTa          >  2:1422105/1‑93 (MQ=255)
                                                                                aaCTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTAt         <  2:2979729/93‑1 (MQ=255)
                                                                                aaCTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTAt         >  2:2627603/1‑93 (MQ=255)
                                                                                  cTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCg       <  2:5550082/93‑1 (MQ=255)
                                                                                   tGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGt      <  2:4125621/93‑1 (MQ=255)
                                                                                   tGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTCAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGt      >  2:3560460/1‑93 (MQ=255)
                                                                                     tACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGttt    <  1:4561766/93‑1 (MQ=255)
                                                                                      aCTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTa   >  1:3164899/1‑93 (MQ=255)
                                                                                      aCTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTa   >  2:4308201/1‑93 (MQ=255)
                                                                                       cTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTaa  <  2:6531100/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAA  >  NC_000913/2330101‑2330280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACGT  >  NC_000913/2330099‑2330284
                                                                                            |                                                                                             
TCACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGC                                                                                              <  SRR4302536.5534657/94‑1 (MQ=60)
 CACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCC                                                                                             <  SRR4302536.4137403/94‑1 (MQ=60)
 CACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCC                                                                                             <  SRR4302536.4596553/94‑1 (MQ=60)
  ACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCG                                                                                            >  SRR4302536.3290749/1‑94 (MQ=60)
   CCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGT                                                                                           >  SRR4302536.1932224/1‑94 (MQ=60)
   CCAGGCTGGGAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGT                                                                                           >  SRR4302536.5920124/1‑94 (MQ=60)
    CAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTT                                                                                          <  SRR4302536.6245565/94‑1 (MQ=60)
    CAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTT                                                                                          >  SRR4302536.2078284/1‑94 (MQ=60)
    CAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTT                                                                                          >  SRR4302536.3747698/1‑94 (MQ=60)
    CAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTT                                                                                          <  SRR4302536.433385/94‑1 (MQ=60)
     AGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTA                                                                                         >  SRR4302536.15968/1‑94 (MQ=60)
      GTCTGTTAATGCGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTAC                                                                                        <  SRR4302536.945296/94‑1 (MQ=60)
      GGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTAC                                                                                        <  SRR4302536.2362787/94‑1 (MQ=60)
       GCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACG                                                                                       <  SRR4302536.1038154/94‑1 (MQ=60)
       GCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACG                                                                                       <  SRR4302536.4660412/94‑1 (MQ=60)
        CTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGG                                                                                      >  SRR4302536.2240228/1‑94 (MQ=60)
          GGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGT                                                                                    <  SRR4302536.1948812/94‑1 (MQ=60)
          GGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGT                                                                                    <  SRR4302536.15745/94‑1 (MQ=60)
          GGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGT                                                                                    <  SRR4302536.6061774/94‑1 (MQ=60)
           GTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTA                                                                                   <  SRR4302536.228367/94‑1 (MQ=60)
           GTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTA                                                                                   <  SRR4302536.6273377/94‑1 (MQ=60)
           GTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACCGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTA                                                                                   >  SRR4302536.5482071/1‑94 (MQ=60)
            TAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTAT                                                                                  <  SRR4302536.2365209/94‑1 (MQ=60)
              ATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGATAACTGTACTGGCCGTTACGGGTATAC                                                                                >  SRR4302536.3109801/1‑94 (MQ=60)
              ATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATAC                                                                                <  SRR4302536.4474448/94‑1 (MQ=60)
              ATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATAC                                                                                <  SRR4302536.2297812/94‑1 (MQ=60)
               TGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACA                                                                               <  SRR4302536.4143937/94‑1 (MQ=60)
               TGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACA                                                                               >  SRR4302536.4873119/1‑94 (MQ=60)
               TGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACA                                                                               >  SRR4302536.4551669/1‑94 (MQ=60)
                GGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAG                                                                              <  SRR4302536.4392611/94‑1 (MQ=60)
                  GTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCA                                                                            >  SRR4302536.930456/1‑94 (MQ=60)
                    TTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACC                                                                          <  SRR4302536.2665879/94‑1 (MQ=60)
                      TGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGA                                                                        >  SRR4302536.5402174/1‑94 (MQ=60)
                      TGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGA                                                                        <  SRR4302536.963247/94‑1 (MQ=60)
                      TGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCACGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGA                                                                        <  SRR4302536.3622446/94‑1 (MQ=60)
                         CCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAA                                                                     >  SRR4302536.2117725/1‑94 (MQ=60)
                         CCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAA                                                                     <  SRR4302536.558336/94‑1 (MQ=60)
                         CCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAA                                                                     >  SRR4302536.5028288/1‑94 (MQ=60)
                         CCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAA                                                                     <  SRR4302536.1729456/94‑1 (MQ=60)
                          CGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAA                                                                    <  SRR4302536.4455740/94‑1 (MQ=60)
                           GTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAG                                                                   >  SRR4302536.1882196/1‑94 (MQ=60)
                           GTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAG                                                                   <  SRR4302536.756888/94‑1 (MQ=60)
                            TTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGT                                                                  <  SRR4302536.2247608/94‑1 (MQ=60)
                             TTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTG                                                                 <  SRR4302536.5196040/94‑1 (MQ=60)
                             TTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTG                                                                 <  SRR4302536.5328745/94‑1 (MQ=60)
                             TTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTG                                                                 <  SRR4302536.6779851/94‑1 (MQ=60)
                               TCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGAT                                                               <  SRR4302536.1874968/94‑1 (MQ=60)
                               TAACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGAT                                                               <  SRR4302536.4839463/94‑1 (MQ=60)
                                CACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATG                                                              >  SRR4302536.182195/1‑94 (MQ=60)
                                CACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATG                                                              <  SRR4302536.6481158/94‑1 (MQ=60)
                                 ACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGT                                                             >  SRR4302536.6154957/1‑94 (MQ=60)
                                  CCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTT                                                            >  SRR4302536.4084497/1‑94 (MQ=60)
                                  CCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTT                                                            >  SRR4302536.4954627/1‑94 (MQ=60)
                                  CCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTT                                                            <  SRR4302536.3726061/94‑1 (MQ=60)
                                    CGGATATCCAGCTGGGGAACGGCACCTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAG                                                          <  SRR4302536.3385854/94‑1 (MQ=60)
                                    CGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAG                                                          <  SRR4302536.2102816/94‑1 (MQ=60)
                                     GGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGG                                                         <  SRR4302536.4649372/94‑1 (MQ=60)
                                     GGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGG                                                         >  SRR4302536.795350/1‑94 (MQ=60)
                                     GGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGG                                                         <  SRR4302536.6345079/94‑1 (MQ=60)
                                      GATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGC                                                        >  SRR4302536.6342965/1‑94 (MQ=60)
                                       ATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCG                                                       <  SRR4302536.548307/94‑1 (MQ=60)
                                       ATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCG                                                       >  SRR4302536.5689319/1‑94 (MQ=60)
                                        TATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGC                                                      >  SRR4302536.1910219/1‑94 (MQ=60)
                                         ATCCAGCTGAGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGGGCA                                                     >  SRR4302536.6745440/1‑94 (MQ=60)
                                          TCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAA                                                    >  SRR4302536.6584482/1‑94 (MQ=60)
                                            CAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAG                                                  <  SRR4302536.3486186/94‑1 (MQ=60)
                                            CAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAG                                                  <  SRR4302536.4231205/94‑1 (MQ=60)
                                             AGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGG                                                 <  SRR4302536.2703271/94‑1 (MQ=60)
                                               CTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAA                                               >  SRR4302536.1203385/1‑94 (MQ=60)
                                                TGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAAT                                              <  SRR4302536.1461888/94‑1 (MQ=60)
                                                 GGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATT                                             <  SRR4302536.3804217/94‑1 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            <  SRR4302536.3874191/94‑1 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            <  SRR4302536.518832/94‑1 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            >  SRR4302536.5101760/1‑94 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            <  SRR4302536.3043289/94‑1 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            >  SRR4302536.5409477/1‑94 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            >  SRR4302536.1506638/1‑94 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTG                                            >  SRR4302536.3495191/1‑94 (MQ=60)
                                                  GGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCCCAAAGGAATTG                                            <  SRR4302536.600823/94‑1 (MQ=60)
                                                    GAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCT                                          >  SRR4302536.6149158/1‑94 (MQ=60)
                                                    GAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCT                                          <  SRR4302536.1610660/94‑1 (MQ=60)
                                                     ATCGGCAGCTTTGATCCCGGCGTGCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTC                                         <  SRR4302536.1105344/94‑1 (MQ=60)
                                                     AACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTC                                         >  SRR4302536.2035445/1‑94 (MQ=60)
                                                     AACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTC                                         >  SRR4302536.5825868/1‑94 (MQ=60)
                                                      ACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCA                                        >  SRR4302536.2621383/1‑94 (MQ=60)
                                                       CGGCATCTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAC                                       <  SRR4302536.3622817/94‑1 (MQ=60)
                                                       CGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCAC                                       >  SRR4302536.5717485/1‑94 (MQ=60)
                                                        GGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACT                                      <  SRR4302536.6300368/94‑1 (MQ=60)
                                                        GGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACT                                      >  SRR4302536.3813433/1‑94 (MQ=60)
                                                          CAACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGG                                    <  SRR4302536.1302306/94‑1 (MQ=60)
                                                           AACTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGA                                   >  SRR4302536.5792677/1‑94 (MQ=60)
                                                             CTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAC                                 >  SRR4302536.5379013/1‑94 (MQ=60)
                                                             CTTTGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAC                                 >  SRR4302536.1251182/1‑94 (MQ=60)
                                                              TTTGATCCCGGCTTGCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGTCGCAAAGGAATTGCTCACTGGAACC                                <  SRR4302536.6415564/94‑1 (MQ=60)
                                                                TGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCCCTGGAACCCG                              >  SRR4302536.5915117/1‑94 (MQ=60)
                                                                TGATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCG                              <  SRR4302536.1046650/94‑1 (MQ=60)
                                                                 GATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGG                             <  SRR4302536.3080893/94‑1 (MQ=60)
                                                                 GATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGG                             >  SRR4302536.4051590/1‑94 (MQ=60)
                                                                 GATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGG                             <  SRR4302536.796654/94‑1 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.6194346/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.1259312/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.4500935/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.874347/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.1603913/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.3793760/1‑94 (MQ=60)
                                                                  ATCCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGGATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGG                            >  SRR4302536.1808673/1‑94 (MQ=60)
                                                                    CCCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCT                          >  SRR4302536.2035339/1‑94 (MQ=60)
                                                                     CCGGCGTTCTGAAAACTGTACTGGCCGTTTCGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTT                         <  SRR4302536.6792795/94‑1 (MQ=60)
                                                                     CCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTT                         <  SRR4302536.1806013/94‑1 (MQ=60)
                                                                     CCGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTT                         <  SRR4302536.6803622/94‑1 (MQ=60)
                                                                      CGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTC                        <  SRR4302536.3643758/94‑1 (MQ=60)
                                                                      CGGCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTC                        <  SRR4302536.1566815/94‑1 (MQ=60)
                                                                        GCGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCAT                      >  SRR4302536.3984720/1‑94 (MQ=60)
                                                                         CGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAACGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAAaccgcgcttgcta                     >  SRR4302536.979162/1‑81 (MQ=60)
                                                                         CGTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAACGGAATTGCTCACTGGAACCCGCGCTTCATA                     >  SRR4302536.2011033/1‑94 (MQ=60)
                                                                          GTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAC                    <  SRR4302536.381984/94‑1 (MQ=60)
                                                                          GTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAC                    >  SRR4302536.5750640/1‑94 (MQ=60)
                                                                          GTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAC                    <  SRR4302536.5716186/94‑1 (MQ=60)
                                                                          GTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAC                    <  SRR4302536.5905581/94‑1 (MQ=60)
                                                                          GTTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATAC                    <  SRR4302536.4777409/94‑1 (MQ=60)
                                                                           TTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACT                   <  SRR4302536.4927660/94‑1 (MQ=60)
                                                                           TTCTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACT                   <  SRR4302536.5798708/94‑1 (MQ=60)
                                                                             CTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGG                 >  SRR4302536.1369525/1‑94 (MQ=60)
                                                                             CTGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGGGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGG                 >  SRR4302536.5225369/1‑94 (MQ=60)
                                                                              TGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGG                >  SRR4302536.4227830/1‑94 (MQ=60)
                                                                              TGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGG                >  SRR4302536.3019398/1‑94 (MQ=60)
                                                                              TGAAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGG                >  SRR4302536.6892345/1‑94 (MQ=60)
                                                                                AAAACTGTCCTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTA              <  SRR4302536.5841206/94‑1 (MQ=60)
                                                                                AAAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTA              <  SRR4302536.247353/94‑1 (MQ=60)
                                                                                 AAACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTAT             >  SRR4302536.4658570/1‑94 (MQ=60)
                                                                                  AACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATC            >  SRR4302536.6364593/1‑94 (MQ=60)
                                                                                   ACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCG           >  SRR4302536.3078416/1‑94 (MQ=60)
                                                                                   ACTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCG           <  SRR4302536.845599/94‑1 (MQ=60)
                                                                                    CTGTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGT          >  SRR4302536.2586952/1‑94 (MQ=60)
                                                                                      GTACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTT        >  SRR4302536.2034170/1‑94 (MQ=60)
                                                                                       TACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTA       >  SRR4302536.3257615/1‑94 (MQ=60)
                                                                                       TACTGGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTA       <  SRR4302536.4698633/94‑1 (MQ=60)
                                                                                           GGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACG   <  SRR4302536.3371041/94‑1 (MQ=60)
                                                                                           GGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACG   >  SRR4302536.1849752/1‑94 (MQ=60)
                                                                                           GGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACG   <  SRR4302536.2738130/94‑1 (MQ=60)
                                                                                           GGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACG   <  SRR4302536.6387894/94‑1 (MQ=60)
                                                                                           GGCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACG   <  SRR4302536.2581022/94‑1 (MQ=60)
                                                                                            GCCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACGT  >  SRR4302536.2738366/1‑94 (MQ=60)
                                                                                            |                                                                                             
TCACCAGGCTGGTAATGGGTTTTGTCCGTTTTCACCCGGATATCCAGCTGGGGAACGGCAACTTTGATCCCGGCGTTCTGAAAACTGTACTGACCGTTACGGGTATACAGCACCGAAAAAGTGATGTTAGGCGCAAAGGAATTGCTCACTGGAACCCGGGCTTCATACTGGGTATCGTTTAAACGT  >  NC_000913/2330099‑2330284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑