Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,331,739 A→G intergenic (‑3922/+617) b2226 ← / ← b2229 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,331,7390AG99.6% 750.6 / NA 229intergenic (‑3922/+617)b2226/b2229hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (94/134);  minor base T (0/1);  total (94/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT  >  NC_000913/2331649‑2331827
                                                                                          |                                                                                        
ggTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGat                                                                                        <  2:6345316/93‑1 (MQ=255)
 gTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGata                                                                                       <  2:2166442/93‑1 (MQ=255)
    tCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaa                                                                                    <  1:378596/93‑1 (MQ=255)
     cGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATaaa                                                                                   <  1:2170001/93‑1 (MQ=255)
       gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATg                                                                                 <  1:4722741/93‑1 (MQ=255)
       gAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATg                                                                                 >  2:1169331/1‑93 (MQ=255)
          acaaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTa                                                                              <  1:4873266/93‑1 (MQ=255)
            aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAcc                                                                            <  1:5044787/93‑1 (MQ=255)
            aaacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAcc                                                                            <  1:3110770/93‑1 (MQ=255)
             aacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAccc                                                                           <  1:2050381/93‑1 (MQ=255)
             aacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAccc                                                                           <  1:4101846/93‑1 (MQ=255)
             aacaCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAccc                                                                           <  1:2374538/93‑1 (MQ=255)
              acacCCCCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCg                                                                          <  2:4235590/93‑1 (MQ=255)
                cccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  2:6100502/92‑1 (MQ=255)
                cccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  1:5068494/92‑1 (MQ=255)
                accaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        >  2:3766864/1‑93 (MQ=255)
                accaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  1:3572211/93‑1 (MQ=255)
                accaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGc                                                                        <  2:5466120/93‑1 (MQ=255)
                 cccccGGCGCCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCt                                                                       <  1:6416493/90‑1 (MQ=255)
                 ccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCt                                                                       <  2:5120720/93‑1 (MQ=255)
                 ccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCt                                                                       >  2:3816783/1‑93 (MQ=255)
                 ccaccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCt                                                                       >  2:1763207/1‑93 (MQ=255)
                  caccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTg                                                                      <  2:1635405/93‑1 (MQ=255)
                   cccGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgc                                                                     <  1:1700572/92‑1 (MQ=255)
                   accGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgc                                                                     >  1:2819732/1‑93 (MQ=255)
                     cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcgg                                                                   <  2:5655777/93‑1 (MQ=255)
                     cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcgg                                                                   <  2:4910308/93‑1 (MQ=255)
                     cgtcgtCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcgg                                                                   <  2:4031695/93‑1 (MQ=255)
                      gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggc                                                                  >  1:3026551/1‑93 (MQ=255)
                      gtcgtcGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggc                                                                  >  1:1500344/1‑93 (MQ=255)
                       tcgtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTgcggcg                                                                 >  2:81773/1‑93 (MQ=255)
                        cgtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATTAATGTTACCCGGCTgcggcgg                                                                >  1:55129/1‑92 (MQ=255)
                         gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgag                                                               >  2:359055/1‑93 (MQ=255)
                         gtcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCAGGCTGCGGCgag                                                               >  1:3508443/1‑93 (MQ=255)
                          tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              <  2:2997485/93‑1 (MQ=255)
                          tcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              <  1:2586588/93‑1 (MQ=255)
                          gcgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCgaga                                                              <  1:2281617/92‑1 (MQ=255)
                           cgCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAt                                                             <  1:4186255/93‑1 (MQ=255)
                             cccGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGa                                                           >  2:6015768/1‑93 (MQ=255)
                             cccGATACACACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGa                                                           <  2:3932536/93‑1 (MQ=255)
                              ccGATACCCACCAACCATCGCCTCGACGAGGGACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGaa                                                          >  1:4832863/1‑93 (MQ=255)
                               cGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGaaa                                                         <  1:5469174/93‑1 (MQ=255)
                                gATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                        <  2:5541426/93‑1 (MQ=255)
                                gATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                        <  1:6059766/93‑1 (MQ=255)
                                gATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAt                                                        >  1:6467601/1‑93 (MQ=255)
                                 aTACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAtt                                                       <  1:4652431/93‑1 (MQ=255)
                                 aTACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAtt                                                       >  1:5357306/1‑93 (MQ=255)
                                 aTACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAtt                                                       <  2:5155353/93‑1 (MQ=255)
                                  tACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGCGGGGAGGAAAtgc                                                      >  2:2314765/1‑91 (MQ=255)
                                   aCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGc                                                     <  1:6646447/93‑1 (MQ=255)
                                    cccACCAGCCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCt                                                    <  1:2704903/93‑1 (MQ=255)
                                      caccaACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTgg                                                  >  1:2311782/1‑93 (MQ=255)
                                        ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                <  2:4667128/93‑1 (MQ=255)
                                        ccaaccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAt                                                <  1:981779/93‑1 (MQ=255)
                                            ccaTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATgcgc                                            >  2:1847573/1‑93 (MQ=255)
                                             caTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGcc                                           >  2:2041224/1‑93 (MQ=255)
                                              aTCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCt                                          <  2:1623588/93‑1 (MQ=255)
                                                 gCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                       <  2:257950/93‑1 (MQ=255)
                                                 gCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTcc                                       <  2:1202943/93‑1 (MQ=255)
                                                  ccTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCa                                      <  2:2389082/93‑1 (MQ=255)
                                                   cTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAg                                     <  2:2687522/93‑1 (MQ=255)
                                                   cTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAg                                     <  1:3962163/93‑1 (MQ=255)
                                                     cgacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtt                                   <  1:768841/93‑1 (MQ=255)
                                                     cgacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtt                                   <  2:6726335/93‑1 (MQ=255)
                                                     cgacgaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGtt                                   <  2:3804779/93‑1 (MQ=255)
                                                         gaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAc                               >  1:6116035/1‑93 (MQ=255)
                                                         gaGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAACCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAc                               >  2:640616/1‑93 (MQ=255)
                                                           ggTACAGTCCCGGCTCTTGTTGGCCGCGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCCGTTTTAccc                             >  1:792701/1‑93 (MQ=255)
                                                           ggTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAccc                             <  2:3355476/93‑1 (MQ=255)
                                                           ggTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAccc                             >  2:5852069/1‑93 (MQ=255)
                                                             tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                           <  1:3234918/93‑1 (MQ=255)
                                                             tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                           <  1:3289743/93‑1 (MQ=255)
                                                             tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                           <  1:6309530/93‑1 (MQ=255)
                                                             tACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                           <  2:497914/93‑1 (MQ=255)
                                                             gaCAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCt                           <  1:5204164/92‑1 (MQ=255)
                                                              aCAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCtt                          >  1:1196016/1‑93 (MQ=255)
                                                              aCAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCtt                          >  2:4161049/1‑93 (MQ=255)
                                                               cAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtg                         <  2:2766294/93‑1 (MQ=255)
                                                                   cccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgctgc                     <  2:4722599/93‑1 (MQ=255)
                                                                   cccGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTtgctgc                     <  2:4824380/93‑1 (MQ=255)
                                                                       gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCt                 <  1:5054153/93‑1 (MQ=255)
                                                                       gCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCt                 >  1:1102054/1‑93 (MQ=255)
                                                                        ctctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTc                <  2:3443442/93‑1 (MQ=255)
                                                                        ctctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTc                >  2:3497559/1‑93 (MQ=255)
                                                                         tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg               >  1:5863006/1‑93 (MQ=255)
                                                                         tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg               >  1:1932223/1‑93 (MQ=255)
                                                                         tctTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCg               >  2:5593721/1‑93 (MQ=255)
                                                                           ttgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGaa             <  2:1333765/93‑1 (MQ=255)
                                                                           ttgttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCaa             >  2:4559617/1‑93 (MQ=255)
                                                                             gttgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGagag           >  1:5222003/1‑90 (MQ=255)
                                                                               tgGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGt         <  1:4001825/93‑1 (MQ=255)
                                                                                ggCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACgggt        >  1:6202524/1‑91 (MQ=255)
                                                                                 gctgagagagaTATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCGCCCAGTTTTCCCCCTTGCTGCGGCTCGAACGGttt       <  1:1452546/90‑1 (MQ=255)
                                                                                   cGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAACGGGTTAg     >  2:4075413/1‑93 (MQ=255)
                                                                                    gagaGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGc    >  1:6576637/1‑93 (MQ=255)
                                                                                    gagaGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGGTAGc    >  1:447854/1‑93 (MQ=255)
                                                                                     agagGGATATAAATGTTACCCGTCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGcc   <  1:4516814/93‑1 (MQ=255)
                                                                                     agagGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACTGTTTATcc   >  1:177239/1‑93 (MQ=255)
                                                                                     agagGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGcc   <  2:6221376/93‑1 (MQ=255)
                                                                                      gagGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGGACGGGTTTGCCt  >  1:416317/1‑93 (MQ=255)
                                                                                          |                                                                                        
GGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT  >  NC_000913/2331649‑2331827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCC  >  NC_000913/2331646‑2331830
                                                                                             |                                                                                           
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                             >  SRR4302536.2658765/1‑94 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                             >  SRR4302536.3108673/1‑94 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                             <  SRR4302536.1694579/94‑1 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                             <  SRR4302536.3281379/94‑1 (MQ=60)
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGG                                                                                             >  SRR4302536.5210414/1‑94 (MQ=60)
  CGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGAT                                                                                           >  SRR4302536.5377174/1‑94 (MQ=60)
    GTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATAT                                                                                         <  SRR4302536.617268/94‑1 (MQ=60)
     TATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATA                                                                                        <  SRR4302536.6086649/94‑1 (MQ=60)
       TCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAA                                                                                      <  SRR4302536.388569/94‑1 (MQ=60)
        CGGAAACAAACACCACCGTCGTCGCCCGATCCCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                     <  SRR4302536.3149196/94‑1 (MQ=60)
        CGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                     <  SRR4302536.637392/94‑1 (MQ=60)
        CGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                     >  SRR4302536.5142609/1‑94 (MQ=60)
        CGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAAT                                                                                     <  SRR4302536.2235336/94‑1 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                   >  SRR4302536.3283694/1‑94 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                   <  SRR4302536.3633624/94‑1 (MQ=60)
          GAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGT                                                                                   <  SRR4302536.4864841/94‑1 (MQ=60)
           AAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTT                                                                                  >  SRR4302536.4719063/1‑94 (MQ=60)
            AACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTA                                                                                 <  SRR4302536.6531241/94‑1 (MQ=60)
             ACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTAC                                                                                <  SRR4302536.5019870/94‑1 (MQ=60)
               AAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCC                                                                              >  SRR4302536.4946379/1‑94 (MQ=60)
               AAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCC                                                                              <  SRR4302536.3201602/94‑1 (MQ=60)
               AAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCC                                                                              <  SRR4302536.5195971/94‑1 (MQ=60)
               AAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCC                                                                              >  SRR4302536.4651680/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             <  SRR4302536.2112129/94‑1 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             >  SRR4302536.6758686/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             <  SRR4302536.6647826/94‑1 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             <  SRR4302536.4223537/94‑1 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             >  SRR4302536.2373520/1‑94 (MQ=60)
                AACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCG                                                                             <  SRR4302536.2446472/94‑1 (MQ=60)
                  CACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGC                                                                           >  SRR4302536.812177/1‑94 (MQ=60)
                   CCCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                          <  SRR4302536.5220263/94‑1 (MQ=60)
                   ACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCT                                                                          <  SRR4302536.3679011/94‑1 (MQ=60)
                    cccccggcgcCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                         <  SRR4302536.6608232/84‑1 (MQ=60)
                    CCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                         <  SRR4302536.3344070/94‑1 (MQ=60)
                    CCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                         <  SRR4302536.2321632/94‑1 (MQ=60)
                    CCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTG                                                                         <  SRR4302536.227369/94‑1 (MQ=60)
                     CACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGC                                                                        <  SRR4302536.5344662/94‑1 (MQ=60)
                     CACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGC                                                                        >  SRR4302536.2903245/1‑94 (MQ=60)
                      CCCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCG                                                                       <  SRR4302536.1749784/94‑1 (MQ=60)
                        CGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGC                                                                     >  SRR4302536.1544603/1‑94 (MQ=60)
                        CGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGC                                                                     >  SRR4302536.3115483/1‑94 (MQ=60)
                          TCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATTAATGTTACCCGGCTGCGGCGG                                                                   >  SRR4302536.56660/1‑94 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAG                                                                  <  SRR4302536.3422664/94‑1 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAG                                                                  >  SRR4302536.3772295/1‑94 (MQ=60)
                           CGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCAGGCTGCGGCGAG                                                                  >  SRR4302536.3613173/1‑94 (MQ=60)
                             TCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAT                                                                <  SRR4302536.5214265/94‑1 (MQ=60)
                             TCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAT                                                                <  SRR4302536.2664292/94‑1 (MQ=60)
                             GCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGAT                                                                <  SRR4302536.2350793/94‑1 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                               <  SRR4302536.5016407/94‑1 (MQ=60)
                              CGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATG                                                               <  SRR4302536.4310649/94‑1 (MQ=60)
                                CCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAA                                                             <  SRR4302536.3361686/94‑1 (MQ=60)
                                CCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGGTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAA                                                             >  SRR4302536.6265963/1‑94 (MQ=60)
                                CCCGATACCCACCAACCATCGCCTCGACGAGGGACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAA                                                             >  SRR4302536.4978299/1‑94 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                           >  SRR4302536.1856220/1‑94 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                           <  SRR4302536.3932815/94‑1 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                           <  SRR4302536.241250/94‑1 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                           >  SRR4302536.6660643/1‑94 (MQ=60)
                                  CGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAAT                                                           <  SRR4302536.5632575/94‑1 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                          <  SRR4302536.6240536/94‑1 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                          <  SRR4302536.178809/94‑1 (MQ=60)
                                   GATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATT                                                          >  SRR4302536.5517166/1‑94 (MQ=60)
                                    ATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTG                                                         <  SRR4302536.4792309/94‑1 (MQ=60)
                                      ACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCT                                                       <  SRR4302536.6844051/94‑1 (MQ=60)
                                       CCCACCAGCCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTG                                                      <  SRR4302536.2785575/94‑1 (MQ=60)
                                        CCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGG                                                     >  SRR4302536.2381941/1‑94 (MQ=60)
                                        CCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGG                                                     >  SRR4302536.6772575/1‑94 (MQ=60)
                                         CACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGA                                                    <  SRR4302536.4218633/94‑1 (MQ=60)
                                          CCCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGAT                                                   <  SRR4302536.421392/94‑1 (MQ=60)
                                           cgagcgagcgcgtgGACGAGGTCCAGTCCCGGCTCTTGTTGGGCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                  <  SRR4302536.5242144/80‑1 (MQ=60)
                                           CCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATG                                                  <  SRR4302536.1010759/94‑1 (MQ=60)
                                            CAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGC                                                 >  SRR4302536.6420966/1‑94 (MQ=60)
                                             AACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCG                                                <  SRR4302536.3059710/94‑1 (MQ=60)
                                               CCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGCTGCGCC                                              >  SRR4302536.6494237/1‑94 (MQ=60)
                                                 ATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTT                                            <  SRR4302536.1537142/94‑1 (MQ=60)
                                                 ATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTT                                            >  SRR4302536.273438/1‑94 (MQ=60)
                                                  TCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTC                                           >  SRR4302536.5234716/1‑94 (MQ=60)
                                                      CTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGT                                       <  SRR4302536.4079804/94‑1 (MQ=60)
                                                       TCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTT                                      <  SRR4302536.5587992/94‑1 (MQ=60)
                                                        CGTCGAGGTACAGTCCCGGCTCGTGTAGGCCGATAGGGATATAAATGTTACGCGGCTGCGGCGAGATGAAATTGCTGGAGGCGCCTTCCAGTTT                                     <  SRR4302536.1672880/94‑1 (MQ=60)
                                                        CGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTT                                     <  SRR4302536.791379/94‑1 (MQ=60)
                                                          ACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTA                                   >  SRR4302536.4580339/1‑94 (MQ=60)
                                                           CGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAC                                  <  SRR4302536.3679145/94‑1 (MQ=60)
                                                           CGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTAC                                  >  SRR4302536.6298328/1‑94 (MQ=60)
                                                             AGGTACAGTCCCGGCTCTTGTTGGCCGCGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCCGTTTTACCC                                >  SRR4302536.815936/1‑94 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                             >  SRR4302536.1231970/1‑94 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                             <  SRR4302536.6497483/94‑1 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                             <  SRR4302536.3329746/94‑1 (MQ=60)
                                                                TACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                             <  SRR4302536.3386561/94‑1 (MQ=60)
                                                                GACAGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTT                             <  SRR4302536.5359297/94‑1 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                          <  SRR4302536.130613/94‑1 (MQ=60)
                                                                   AGTCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCT                          >  SRR4302536.4864695/1‑94 (MQ=60)
                                                                     TCCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGC                        <  SRR4302536.1964575/94‑1 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                       >  SRR4302536.1539480/1‑94 (MQ=60)
                                                                      CCCGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCG                       <  SRR4302536.5049188/94‑1 (MQ=60)
                                                                        CGGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGC                     >  SRR4302536.1150636/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                    >  SRR4302536.1135136/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                    >  SRR4302536.786352/1‑94 (MQ=60)
                                                                         GGCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCT                    <  SRR4302536.2099532/94‑1 (MQ=60)
                                                                          GCTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTC                   <  SRR4302536.5205569/94‑1 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                  >  SRR4302536.1990329/1‑94 (MQ=60)
                                                                           CTCTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCG                  >  SRR4302536.6037671/1‑94 (MQ=60)
                                                                            TATTGTTGGCCGAGAGGGATATAAATGTCACCCGCCTGCGCCGAGAAGAAATTGCTGGATGCCACTCCCAGTTTTACCCCTTGCTGCGGCTCGA                 <  SRR4302536.2881582/94‑1 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGGA                >  SRR4302536.1664618/1‑94 (MQ=60)
                                                                             CTTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAA                <  SRR4302536.2488662/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC               <  SRR4302536.5205165/94‑1 (MQ=60)
                                                                              TTGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAC               <  SRR4302536.4846323/94‑1 (MQ=60)
                                                                               TGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAgag              >  SRR4302536.5377599/1‑91 (MQ=60)
                                                                               TGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACG              <  SRR4302536.885229/94‑1 (MQ=60)
                                                                               TGTTGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACG              >  SRR4302536.4170535/1‑94 (MQ=60)
                                                                                  TGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTT           <  SRR4302536.4120567/94‑1 (MQ=60)
                                                                                  TGGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGT           >  SRR4302536.6387272/1‑94 (MQ=60)
                                                                                   GGCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTT          <  SRR4302536.542691/94‑1 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA         <  SRR4302536.5782511/94‑1 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA         <  SRR4302536.2569557/94‑1 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTA         <  SRR4302536.5600687/94‑1 (MQ=60)
                                                                                    GCCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTAGCTGCGGCTCGAACGGTTTA         <  SRR4302536.6670526/94‑1 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAG        >  SRR4302536.2157924/1‑94 (MQ=60)
                                                                                     CCGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCCAACGGgttaa        >  SRR4302536.4523670/1‑89 (MQ=60)
                                                                                      CGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGC       >  SRR4302536.6772367/1‑94 (MQ=60)
                                                                                      CGAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGGTAGC       >  SRR4302536.459625/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACTGTTTATCC      >  SRR4302536.181898/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCC      >  SRR4302536.5654450/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCC      <  SRR4302536.407132/94‑1 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGCC      >  SRR4302536.4293739/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGGTTAGCC      >  SRR4302536.712188/1‑94 (MQ=60)
                                                                                       GAGAGGGATATAAATGTTACCCGGCTG‑GGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT     >  SRR4302536.296133/1‑94 (MQ=60)
                                                                                       GAGAG‑‑ATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTG    <  SRR4302536.790606/94‑1 (MQ=60)
                                                                                        AGAGGGATATAAATGTTACCCGTCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT     <  SRR4302536.4652295/94‑1 (MQ=60)
                                                                                        AGAGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGGACGGGTTTGCCT     >  SRR4302536.427295/1‑94 (MQ=60)
                                                                                        AGAGGGATATAAATGTTACACGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAAagggtttgact     >  SRR4302536.3733620/1‑83 (MQ=60)
                                                                                        AGAGGGATATAAACGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCT     <  SRR4302536.2879508/94‑1 (MQ=60)
                                                                                          AGGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGGACGGGTTAGTCTGC   >  SRR4302536.2990454/1‑94 (MQ=60)
                                                                                           GGGATATAAATGTTACCCGGCTGCGGCGGGATGAAATTGCTGGGTGCGGCTTCCAGTTTTACCCCTTGCTGCGGgtcggctggcttcgcctcgc  >  SRR4302536.5352807/1‑74 (MQ=60)
                                                                                           GGGATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTTGCCTGCC  >  SRR4302536.5897099/1‑94 (MQ=60)
                                                                                             |                                                                                           
CACGGTATCGGAAACAAACACCACCGTCGTCGCCCGATACCCACCAACCATCGCCTCGACGAGGTACAGTCCCGGCTCTTGTTGGCCGAGAGGAATATAAATGTTACCCGGCTGCGGCGAGATGAAATTGCTGGATGCGCCTTCCAGTTTTACCCCTTGCTGCGGCTCGAACGGTTTAGCCTGCC  >  NC_000913/2331646‑2331830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑