Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1475 266.7 13469680 98.2% 13227225 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,280 T→C T109A (ACC→GCC)  b2335 ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2800TC99.6% 713.3 / ‑5.1 223T109A (ACC→GCC) b2335putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (98/124);  total (98/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 228 total aligned reads displayed.

GTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449191‑2449369
                                                                                         |                                                                                         
gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        >  1:1646937/1‑93 (MQ=255)
 ttATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGata                                                                                       >  2:2691423/1‑93 (MQ=255)
  tATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGatat                                                                                      <  1:1524531/93‑1 (MQ=255)
  tATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGatat                                                                                      >  2:4528964/1‑93 (MQ=255)
   aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     >  2:650664/1‑93 (MQ=255)
   aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     <  1:9169/93‑1 (MQ=255)
   aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     >  1:2400919/1‑93 (MQ=255)
    ttGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTg                                                                                    >  1:5789471/1‑93 (MQ=255)
     tGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTgg                                                                                   <  1:1177648/93‑1 (MQ=255)
     tGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTgg                                                                                   <  2:759758/93‑1 (MQ=255)
      gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGt                                                                                  >  1:3210326/1‑93 (MQ=255)
      gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGt                                                                                  <  2:3511904/93‑1 (MQ=255)
      gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGt                                                                                  >  2:5751901/1‑93 (MQ=255)
       atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  2:6260670/93‑1 (MQ=255)
       atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 >  1:4327862/1‑93 (MQ=255)
        tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAc                                                                                >  1:1627720/1‑93 (MQ=255)
          ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              <  2:1742693/93‑1 (MQ=255)
          ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              <  2:3702513/93‑1 (MQ=255)
            aaaTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAg                                                                            <  2:93197/93‑1 (MQ=255)
              aTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTa                                                                          <  2:3366202/93‑1 (MQ=255)
              aTGGCGCCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTCGTACTGAgga                                                                          >  1:5727564/1‑91 (MQ=255)
                ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAcc                                                                        >  2:5261795/1‑93 (MQ=255)
                ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAcc                                                                        <  1:5054343/93‑1 (MQ=255)
                ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAcc                                                                        >  1:4790737/1‑93 (MQ=255)
                  cGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGt                                                                      >  2:637603/1‑93 (MQ=255)
                   gTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGtt                                                                     >  2:2300774/1‑93 (MQ=255)
                    tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    >  2:1222541/1‑93 (MQ=255)
                     ccAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTc                                                                   <  2:4068459/93‑1 (MQ=255)
                     ccAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTc                                                                   <  1:1071462/93‑1 (MQ=255)
                     ccAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTc                                                                   >  1:3277659/1‑93 (MQ=255)
                       aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGc                                                                 >  2:5683623/1‑93 (MQ=255)
                        gCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGcc                                                                >  1:2187144/1‑93 (MQ=255)
                          tATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGt                                                              <  2:2077581/93‑1 (MQ=255)
                          tATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGt                                                              <  2:4179130/93‑1 (MQ=255)
                            ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                            >  1:1299857/1‑93 (MQ=255)
                            ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                            >  1:6462333/1‑93 (MQ=255)
                              ttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAt                                                          >  1:5683059/1‑93 (MQ=255)
                               tCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATc                                                         >  1:5076578/1‑93 (MQ=255)
                                cACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTACTCg                                                        >  1:2044200/1‑93 (MQ=255)
                                  ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                      <  2:1321051/93‑1 (MQ=255)
                                  ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                      >  2:2763891/1‑93 (MQ=255)
                                  ccGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGtt                                                      >  1:450275/1‑93 (MQ=255)
                                   cGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTg                                                     <  1:2646353/93‑1 (MQ=255)
                                    gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGttaa                                                    >  2:5455978/1‑91 (MQ=255)
                                    gACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGa                                                    >  1:2531526/1‑93 (MQ=255)
                                       ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                                 >  1:1969525/1‑93 (MQ=255)
                                         cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               <  2:3228772/93‑1 (MQ=255)
                                          aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                              >  1:4487864/1‑93 (MQ=255)
                                           aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                             <  1:4023082/93‑1 (MQ=255)
                                           aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                             >  1:759025/1‑93 (MQ=255)
                                           aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                             >  2:1114922/1‑93 (MQ=255)
                                            aCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGc                                            >  1:123221/1‑93 (MQ=255)
                                              aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                          <  1:1017910/93‑1 (MQ=255)
                                               aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                         <  1:3858060/93‑1 (MQ=255)
                                               aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGCACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                         >  1:2768942/1‑93 (MQ=255)
                                                tGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTCATCGTTGAGGTATTGCCCtt                                        >  1:1902079/1‑93 (MQ=255)
                                                tGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCtt                                        >  1:3610919/1‑93 (MQ=255)
                                                tGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCtt                                        >  2:1851388/1‑93 (MQ=255)
                                                 gAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTa                                       <  1:338379/93‑1 (MQ=255)
                                                  aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGTTATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  1:5857449/93‑1 (MQ=255)
                                                  aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  1:5891078/93‑1 (MQ=255)
                                                  aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  2:3095967/93‑1 (MQ=255)
                                                  aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  2:2850116/93‑1 (MQ=255)
                                                  aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  1:542541/93‑1 (MQ=255)
                                                    ttATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                    >  2:5329999/1‑93 (MQ=255)
                                                     tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   <  2:5248869/93‑1 (MQ=255)
                                                      aTCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTg                                  <  2:676848/93‑1 (MQ=255)
                                                       tCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGc                                 <  2:198815/93‑1 (MQ=255)
                                                         ggCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAt                               >  1:6236563/1‑93 (MQ=255)
                                                         ggCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAt                               <  1:4647772/93‑1 (MQ=255)
                                                           cgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATcc                             >  2:5813470/1‑93 (MQ=255)
                                                           cgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATcc                             <  2:6080184/93‑1 (MQ=255)
                                                             cTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGCTTCGCCGTTAATCGCTGAGGCCTTGCCCTTAAGTTGCATCCGc                           >  2:1594728/1‑93 (MQ=255)
                                                              tATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCa                          >  1:5178062/1‑93 (MQ=255)
                                                               aTTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCaa                         <  1:3773578/93‑1 (MQ=255)
                                                                ttttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAg                        >  2:1437965/1‑93 (MQ=255)
                                                                 tttCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  2:1452619/93‑1 (MQ=255)
                                                                 tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  1:3475223/93‑1 (MQ=255)
                                                                 tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  2:5535262/93‑1 (MQ=255)
                                                                 tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  2:244912/93‑1 (MQ=255)
                                                                    cTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGt                    <  1:4182306/93‑1 (MQ=255)
                                                                    cTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGt                    <  2:5166008/93‑1 (MQ=255)
                                                                     tATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt                   <  1:412891/93‑1 (MQ=255)
                                                                      aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  >  2:379432/1‑93 (MQ=255)
                                                                        ccGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGc                <  2:4270679/93‑1 (MQ=255)
                                                                         cGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCa               >  2:5098143/1‑93 (MQ=255)
                                                                           aaTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg             <  2:5852014/93‑1 (MQ=255)
                                                                            aTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGa            >  1:554311/1‑93 (MQ=255)
                                                                            aTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGa            <  2:2130074/93‑1 (MQ=255)
                                                                            aTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGa            <  2:4211104/93‑1 (MQ=255)
                                                                              gCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACt          <  1:6038690/93‑1 (MQ=255)
                                                                               cccAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTg         <  2:587933/93‑1 (MQ=255)
                                                                                 cAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCTTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGtt       <  1:769996/93‑1 (MQ=255)
                                                                                 cAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGtt       <  1:2855771/93‑1 (MQ=255)
                                                                                 cAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGtt       >  2:1555987/1‑93 (MQ=255)
                                                                                  aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      <  2:3657421/93‑1 (MQ=255)
                                                                                  aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      <  2:4733160/93‑1 (MQ=255)
                                                                                    cccGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTtgt    <  2:1076409/93‑1 (MQ=255)
                                                                                     ccGGCGATATTGGTACTGAGTACCGTTTCGCGGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTtgtg   <  1:6020276/93‑1 (MQ=255)
                                                                                      cGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGc  <  1:2901406/93‑1 (MQ=255)
                                                                                         |                                                                                         
GTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449191‑2449369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  >  NC_000913/2449190‑2449373
                                                                                          |                                                                                             
GGTTGTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGAT                                                                                            <  SRR4302536.1110467/94‑1 (MQ=60)
GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGAT                                                                                            >  SRR4302536.1694825/1‑94 (MQ=60)
  TTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAT                                                                                          >  SRR4302536.3633025/1‑94 (MQ=60)
   TATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATT                                                                                         >  SRR4302536.2473581/1‑94 (MQ=60)
   TATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATT                                                                                         <  SRR4302536.1569398/94‑1 (MQ=60)
    ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTG                                                                                        <  SRR4302536.9430/94‑1 (MQ=60)
    ATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTG                                                                                        >  SRR4302536.5961822/1‑94 (MQ=60)
      TGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGT                                                                                      <  SRR4302536.1213103/94‑1 (MQ=60)
      TGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGT                                                                                      >  SRR4302536.3304489/1‑94 (MQ=60)
       GATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTA                                                                                     >  SRR4302536.4457465/1‑94 (MQ=60)
        ATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAC                                                                                    >  SRR4302536.1675104/1‑94 (MQ=60)
         TATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACT                                                                                   <  SRR4302536.2578588/94‑1 (MQ=60)
          ATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTG                                                                                  >  SRR4302536.126828/1‑94 (MQ=60)
              AATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTA                                                                              >  SRR4302536.4635660/1‑94 (MQ=60)
              AATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTA                                                                              >  SRR4302536.3285034/1‑94 (MQ=60)
              AATGGCGCCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTCGTACTGAGGA                                                                              >  SRR4302536.5897906/1‑94 (MQ=60)
               ATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAC                                                                             <  SRR4302536.6199409/94‑1 (MQ=60)
               ATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAC                                                                             <  SRR4302536.457973/94‑1 (MQ=60)
                TGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACC                                                                            >  SRR4302536.4934951/1‑94 (MQ=60)
                TGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACC                                                                            <  SRR4302536.4402467/94‑1 (MQ=60)
                TGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACC                                                                            <  SRR4302536.2096541/94‑1 (MQ=60)
                 GGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCG                                                                           <  SRR4302536.5205763/94‑1 (MQ=60)
                 GGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCG                                                                           >  SRR4302536.1835746/1‑94 (MQ=60)
                    GTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTT                                                                        <  SRR4302536.5937318/94‑1 (MQ=60)
                     TCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTC                                                                       >  SRR4302536.3373998/1‑94 (MQ=60)
                     TCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTC                                                                       <  SRR4302536.315023/94‑1 (MQ=60)
                     TCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTC                                                                       >  SRR4302536.6824092/1‑94 (MQ=60)
                      CCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCG                                                                      <  SRR4302536.1103514/94‑1 (MQ=60)
                       CAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGC                                                                     >  SRR4302536.1423279/1‑94 (MQ=60)
                        AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCC                                                                    >  SRR4302536.2253091/1‑94 (MQ=60)
                        AGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCC                                                                    >  SRR4302536.5639938/1‑94 (MQ=60)
                           TATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTT                                                                 >  SRR4302536.904169/1‑94 (MQ=60)
                           TATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTT                                                                 <  SRR4302536.2120208/94‑1 (MQ=60)
                            ATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTA                                                                >  SRR4302536.6655243/1‑94 (MQ=60)
                            ATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTA                                                                >  SRR4302536.1338701/1‑94 (MQ=60)
                              TTTCACCGACGGCAAGCAATGAGTTATCGGCACTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCTGTTAAT                                                              <  SRR4302536.926184/94‑1 (MQ=60)
                              TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                              <  SRR4302536.3722141/94‑1 (MQ=60)
                              TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                              <  SRR4302536.2923371/94‑1 (MQ=60)
                              TTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAAT                                                              >  SRR4302536.5852047/1‑94 (MQ=60)
                               TTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATC                                                             >  SRR4302536.5228528/1‑94 (MQ=60)
                               TTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATC                                                             >  SRR4302536.6065465/1‑94 (MQ=60)
                                TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTACTCG                                                            >  SRR4302536.2105747/1‑94 (MQ=60)
                                TCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCG                                                            >  SRR4302536.1871225/1‑94 (MQ=60)
                                  ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          <  SRR4302536.1972573/94‑1 (MQ=60)
                                  ACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTT                                                          >  SRR4302536.462104/1‑94 (MQ=60)
                                    CGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGA                                                        <  SRR4302536.2725640/94‑1 (MQ=60)
                                    CGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGA                                                        >  SRR4302536.2607821/1‑94 (MQ=60)
                                     GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       <  SRR4302536.329554/94‑1 (MQ=60)
                                     GACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAG                                                       >  SRR4302536.970099/1‑94 (MQ=60)
                                      ACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGG                                                      <  SRR4302536.486426/94‑1 (MQ=60)
                                       CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                                     >  SRR4302536.5511484/1‑94 (MQ=60)
                                       CGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGT                                                     >  SRR4302536.2028746/1‑94 (MQ=60)
                                         GCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAT                                                   <  SRR4302536.4482225/94‑1 (MQ=60)
                                          CAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATT                                                  >  SRR4302536.4622635/1‑94 (MQ=60)
                                           AAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTG                                                 >  SRR4302536.781277/1‑94 (MQ=60)
                                            AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGC                                                <  SRR4302536.4142442/94‑1 (MQ=60)
                                            AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGC                                                <  SRR4302536.4068509/94‑1 (MQ=60)
                                            AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGC                                                >  SRR4302536.2176871/1‑94 (MQ=60)
                                            AACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGC                                                >  SRR4302536.126473/1‑94 (MQ=60)
                                             ACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCC                                               >  SRR4302536.5420416/1‑94 (MQ=60)
                                             ACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCC                                               <  SRR4302536.5926783/94‑1 (MQ=60)
                                              CAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCC                                              <  SRR4302536.1273840/94‑1 (MQ=60)
                                               AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                             <  SRR4302536.1048023/94‑1 (MQ=60)
                                               AATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGCACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCT                                             >  SRR4302536.2851265/1‑94 (MQ=60)
                                                ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTCATCGTTGAGGTATTGCCCTT                                            >  SRR4302536.1959344/1‑94 (MQ=60)
                                                ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                            >  SRR4302536.3718891/1‑94 (MQ=60)
                                                ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                            <  SRR4302536.3972881/94‑1 (MQ=60)
                                                ATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTT                                            <  SRR4302536.653386/94‑1 (MQ=60)
                                                 TGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTA                                           >  SRR4302536.6017988/1‑94 (MQ=60)
                                                  GAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAA                                          <  SRR4302536.347331/94‑1 (MQ=60)
                                                   AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGTTATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302536.6031948/94‑1 (MQ=60)
                                                   AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302536.556381/94‑1 (MQ=60)
                                                   AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302536.6066501/94‑1 (MQ=60)
                                                   AGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAG                                         <  SRR4302536.6841832/94‑1 (MQ=60)
                                                     TTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTT                                       >  SRR4302536.6222638/1‑94 (MQ=60)
                                                     TTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTT                                       <  SRR4302536.5211584/94‑1 (MQ=60)
                                                       ATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCATTAAATTGC                                     >  SRR4302536.2346639/1‑94 (MQ=60)
                                                        TCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCA                                    <  SRR4302536.1239767/94‑1 (MQ=60)
                                                         CGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAT                                   >  SRR4302536.6422362/1‑94 (MQ=60)
                                                         CGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCAT                                   <  SRR4302536.2670750/94‑1 (MQ=60)
                                                          GGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATC                                  <  SRR4302536.4787511/94‑1 (MQ=60)
                                                           GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 <  SRR4302536.4723130/94‑1 (MQ=60)
                                                           GCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCC                                 >  SRR4302536.4254644/1‑94 (MQ=60)
                                                              CTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCA                              >  SRR4302536.504455/1‑94 (MQ=60)
                                                              CTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCA                              >  SRR4302536.5332535/1‑94 (MQ=60)
                                                               TATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAA                             >  SRR4302536.3667909/1‑94 (MQ=60)
                                                               TATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAA                             <  SRR4302536.407226/94‑1 (MQ=60)
                                                                ATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAG                            <  SRR4302536.1086639/94‑1 (MQ=60)
                                                                ATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAG                            <  SRR4302536.3886213/94‑1 (MQ=60)
                                                                  TTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTC                          <  SRR4302536.3578814/94‑1 (MQ=60)
                                                                    TCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGT                        <  SRR4302536.1683876/94‑1 (MQ=60)
                                                                    TCTATCCGAAGGCCCAGCCCGGCGATATTGGTAGTGAGGACCGTTTCGCCGTTACACGTTGAGGGCTTGCCCTTAAGTTGCATCCGCAAGTact                        >  SRR4302536.5906726/1‑91 (MQ=59)
                                                                     CTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTT                       <  SRR4302536.4306547/94‑1 (MQ=60)
                                                                      TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      >  SRR4302536.2748870/1‑94 (MQ=60)
                                                                      TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      <  SRR4302536.423787/94‑1 (MQ=60)
                                                                      TATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGGTA                      >  SRR4302536.3920039/1‑94 (MQ=60)
                                                                      TACCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTA                      <  SRR4302536.3381853/94‑1 (MQ=60)
                                                                       ATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG                     >  SRR4302536.1586050/1‑94 (MQ=60)
                                                                       ATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAG                     >  SRR4302536.3534901/1‑94 (MQ=60)
                                                                        TCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGC                    <  SRR4302536.792382/94‑1 (MQ=60)
                                                                           GAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAG                 <  SRR4302536.5148481/94‑1 (MQ=60)
                                                                            AATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGA                >  SRR4302536.568434/1‑94 (MQ=60)
                                                                            AATGCCCAGCCCGGCGATATTGGTACTGAGAACCGTTTCGCCGCTAATCGTTGAGGTATTGCCCTTAAGATGCATCCGCAAGTCGTTAGCAAGA                >  SRR4302536.2819817/1‑94 (MQ=60)
                                                                             ATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAC               >  SRR4302536.2331731/1‑94 (MQ=60)
                                                                             ATGCCCAGCCCGGCGAGATTGGTACTGAGTACCGTTTCGTCGTTAATtgttttggtgttgcgctttagttgtattcttcagtcggtttctagat               >  SRR4302536.3513973/1‑47 (MQ=60)
                                                                               GCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTG             <  SRR4302536.6218788/94‑1 (MQ=60)
                                                                                 CCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT           <  SRR4302536.6204046/94‑1 (MQ=60)
                                                                                 CCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTT           <  SRR4302536.5857254/94‑1 (MQ=60)
                                                                                  CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCTTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          <  SRR4302536.792575/94‑1 (MQ=60)
                                                                                  CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          >  SRR4302536.4178007/1‑94 (MQ=60)
                                                                                  CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          <  SRR4302536.6124055/94‑1 (MQ=60)
                                                                                  CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          <  SRR4302536.5260724/94‑1 (MQ=60)
                                                                                  CAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTT          <  SRR4302536.2940211/94‑1 (MQ=60)
                                                                                    GCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGT        <  SRR4302536.5713504/94‑1 (MQ=60)
                                                                                     CCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTG       >  SRR4302536.1195152/1‑94 (MQ=60)
                                                                                      CCGGCGATATTGGTACTGAGTACCGTTTCGCGGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC      <  SRR4302536.6199767/94‑1 (MQ=60)
                                                                                       CGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCA     <  SRR4302536.2966255/94‑1 (MQ=60)
                                                                                       CGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCA     <  SRR4302536.2987021/94‑1 (MQ=60)
                                                                                        GGCGATATCGGTACTGAGTACCGTTTCGCGGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTAGTTAGCAAGACTGTTTGTGCAG    <  SRR4302536.2274314/94‑1 (MQ=60)
                                                                                         GCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGT   <  SRR4302536.1451637/94‑1 (MQ=60)
                                                                                         GCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGT   <  SRR4302536.6698246/94‑1 (MQ=60)
                                                                                         GCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGT   <  SRR4302536.5774231/94‑1 (MQ=60)
                                                                                          CGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  <  SRR4302536.3363189/94‑1 (MQ=60)
                                                                                          |                                                                                             
GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGCAGTT  >  NC_000913/2449190‑2449373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑